Standard name
Human Ortholog
Description Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; IGO1 has a paralog, IGO2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.98 0.9 0.61 0.68 0.52 0.47 – 0.4 0.41 0.27 0.35 0.97 0.97 0.97 0.81 0.93 0.85 0.91 0.86 0.95 0.93 0.87 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0 – 0.19 0.15 0.2 0.18 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0.05 0.11 0.07 0.11 0.16 – 0.13 0.17 0.24 0.18 0 0 0 0 0 0 0 0.08 0 0 0.07 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0.29 0.46 0.47 0.38 0.46 – 0.46 0.35 0.45 0.39 0 0.08 0.09 0.36 0.18 0.23 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 1 0 0 0 1 – 1 2 2 0 0 0 0 0 1 1 0 0 0 1 7 6
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 0 0 0 0 – 0 2 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 1 1 2 0 0 0 0 0 0
Cytoplasm 106 157 19 118 205 61 151 – 57 88 25 29 388 885 774 319 282 306 105 136 36 510 667 820
Endoplasmic Reticulum 0 1 0 0 0 0 0 – 0 2 0 0 2 0 1 10 12 7 1 1 0 2 2 5
Endosome 1 0 0 3 2 0 4 – 2 1 0 0 0 0 2 0 0 0 0 1 0 2 4 6
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 2 0 0 0 1 1 3
Mitochondria 1 1 0 1 0 11 14 – 28 32 19 15 0 2 0 4 1 4 1 0 0 0 0 4
Nucleus 0 2 1 22 20 13 52 – 19 36 22 15 5 7 7 4 10 5 2 12 0 20 55 33
Nuclear Periphery 1 0 0 0 0 1 0 – 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 2
Nucleolus 0 0 0 0 0 1 0 – 0 0 1 0 0 0 0 0 0 1 1 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 5
Vac/Vac Membrane 2 8 6 89 141 45 148 – 66 76 42 33 16 70 70 143 54 82 1 4 0 7 19 13
Unique Cell Count 110 161 21 192 300 118 321 144 216 93 84 399 914 798 392 304 359 116 159 39 551 768 909
Labelled Cell Count 111 170 27 233 368 132 370 174 240 112 93 411 964 854 481 361 410 116 159 39 551 768 909


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.2 9.8 9.4 9.2 9.6 5.8 8.1 – 7.0 7.1 6.9 6.8 10.2 9.4 11.4 15.0 14.9 15.1 8.7 8.5 10.1
Std Deviation (1e-4) 1.5 1.5 1.4 1.3 1.7 1.3 1.6 – 1.5 1.7 1.5 1.7 1.5 1.4 1.9 3.8 3.8 3.6 1.5 2.4 2.4
Intensity Change (Log2) – – – -0.03 0.02 -0.7 -0.21 – -0.42 -0.4 -0.45 -0.47 0.12 0.01 0.27 0.68 0.66 0.68 -0.11 -0.14 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.6 -2.1 -3.3 -3.9 – -4.4 -4.4 -5.4 -4.6 1.7 1.6 1.7 -1.1 0.4 -0.7
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 1.6 1.6 0.8 1.6 – 1.5 0.6 1.4 0.9 0 0 0 0.7 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.4679 5.3661 4.4629 4.313 3.6532 4.6422 5.9915 7.9703 6.8505 7.1159 5.9928 6.8644 7.5766 9.6211 8.1647 8.0623 7.2909 8.0687
Actin 0.0134 0.0003 0.0103 0.0011 0.0189 0.0016 0.0103 0.0001 0.0065 0.0002 0.0067 0.0023 0.0156 0.0002 0.0093 0.0055 0.0268 0.0008
Bud 0.0003 0.0002 0.0001 0.0004 0.0006 0.0001 0.0003 0.0001 0.0003 0.0002 0.0002 0.0003 0.0002 0.0001 0.0004 0.0002 0.0002 0.0035
Bud Neck 0.0019 0.0003 0.0006 0.0021 0.0052 0.0015 0.0005 0.0002 0.0008 0.001 0.0005 0.0017 0.0006 0.0003 0.0014 0.0009 0.0012 0.0028
Bud Periphery 0.0004 0 0 0.0001 0.0005 0.0001 0.0003 0 0.0002 0 0.0002 0.0004 0.0001 0 0.0003 0.0001 0.0001 0.0011
Bud Site 0.0007 0.0004 0.0002 0.0007 0.0012 0.0001 0.0005 0.0001 0.0005 0.0001 0.0005 0.0007 0.0004 0.0002 0.0092 0.0003 0.0003 0.0007
Cell Periphery 0.0001 0 0 0.0001 0.0001 0 0 0 0 0 0 0.0001 0 0 0.0001 0 0.0001 0
Cytoplasm 0.6502 0.8382 0.8437 0.8965 0.7823 0.7351 0.7265 0.8741 0.8233 0.8942 0.8712 0.7419 0.6935 0.9105 0.8746 0.9394 0.7854 0.7453
Cytoplasmic Foci 0.0077 0.0012 0.0027 0.0016 0.0099 0.0024 0.0101 0.0005 0.0039 0.0008 0.0251 0.0032 0.0153 0.0005 0.0091 0.0115 0.0073 0.0023
Eisosomes 0.0001 0 0 0 0.0001 0 0.0001 0 0.0001 0 0 0.0001 0.0001 0 0 0 0.0005 0
Endoplasmic Reticulum 0.0039 0.0015 0.0035 0.0015 0.0092 0.0027 0.0028 0.0009 0.0022 0.0007 0.0012 0.0021 0.0038 0.0009 0.0013 0.0042 0.0038 0.0018
Endosome 0.0163 0.0009 0.0038 0.001 0.0141 0.0044 0.0124 0.0003 0.0038 0.0004 0.0054 0.0166 0.0107 0.0002 0.004 0.0088 0.0157 0.0017
Golgi 0.0025 0 0.0003 0.0001 0.0009 0.0005 0.0094 0 0.0007 0 0.0047 0.0081 0.0023 0 0.0016 0.0007 0.0058 0.0001
Lipid Particles 0.0024 0 0.0002 0 0.0007 0.0013 0.0102 0 0.0004 0 0.0166 0.0121 0.0057 0 0.0018 0.0004 0.0192 0
Mitochondria 0.0019 0.0002 0.0004 0.0002 0.0008 0.0004 0.0066 0.0001 0.0006 0.0002 0.0007 0.0097 0.0006 0.0001 0.0011 0.0004 0.0027 0.0003
None 0.0067 0.0025 0.0077 0.0019 0.0139 0.0022 0.0029 0.0018 0.0097 0.0009 0.0027 0.0014 0.0148 0.0012 0.0025 0.0058 0.0118 0.0012
Nuclear Periphery 0.0216 0.0069 0.0103 0.0026 0.0312 0.0134 0.0114 0.004 0.0068 0.0036 0.0023 0.0077 0.0096 0.0029 0.0031 0.0023 0.0166 0.0062
Nucleolus 0.0004 0.0001 0.0001 0.0001 0.0007 0.0003 0.0001 0 0.0002 0 0.0001 0.0064 0.0002 0 0.0001 0 0.0017 0.0001
Nucleus 0.2559 0.1436 0.111 0.0882 0.086 0.2272 0.1867 0.116 0.1346 0.0959 0.0185 0.1755 0.215 0.082 0.0724 0.0165 0.0595 0.2282
Peroxisomes 0.006 0 0.0002 0.0001 0.0004 0.0003 0.0048 0 0.0004 0 0.0312 0.0009 0.0039 0 0.0025 0.0006 0.0307 0.0002
Punctate Nuclear 0.0043 0.0025 0.0033 0.001 0.0206 0.0049 0.0027 0.0012 0.0017 0.0012 0.0115 0.0029 0.0067 0.0006 0.0047 0.0015 0.0084 0.0031
Vacuole 0.0025 0.0007 0.0011 0.0006 0.002 0.0012 0.0011 0.0003 0.003 0.0003 0.0004 0.0042 0.0007 0.0002 0.0003 0.0007 0.0008 0.0005
Vacuole Periphery 0.001 0.0001 0.0003 0.0001 0.0008 0.0004 0.0004 0.0001 0.0005 0.0001 0.0001 0.0017 0.0002 0 0.0001 0.0002 0.0012 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.9096 13.6186 23.0985 41.8423 31.0389 21.7514 40.3257 59.0811 42.2749 48.3647
Translational Efficiency 1.3052 3.2752 1.5866 0.8176 1.5302 2.9371 1.0938 0.9574 1.2565 1.3516

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
579 525 2069 93 1609 1524 527 1176 2188 2049 2596 1269

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 872.77 1072.81 1120.66 1480.70 924.93 982.45 1229.30 1130.13 911.13 1005.60 1142.71 1155.82
Standard Deviation 124.61 158.36 163.29 260.54 129.00 132.25 183.86 220.18 129.91 144.88 173.27 241.35
Intensity Change Log 2 — 0.297721 0.360675 0.762606 — 0.087040 0.410421 0.289073 — 0.193169 0.386484 0.538356

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000327 0.002070 0.000199 0.000671 0.000234 0.000309 0.000493 0.000327 0.000259 0.000760 0.000259 0.000352
Bud Neck 0.001473 0.001896 0.003099 0.002967 0.001293 0.004182 0.001946 0.002461 0.001340 0.003596 0.002865 0.002498
Bud Site 0.004152 0.003945 0.003155 0.005085 0.002286 0.006809 0.003940 0.008951 0.002780 0.006075 0.003314 0.008667
Cell Periphery 0.000200 0.000106 0.000072 0.000217 0.000144 0.000080 0.000146 0.000222 0.000159 0.000087 0.000087 0.000221
Cytoplasm 0.588439* 0.659868* 0.416681* 0.305950* 0.568225* 0.557074* 0.450356* 0.629122* 0.573574* 0.583412* 0.423517* 0.605438*
Cytoplasmic Foci 0.069248 0.030519 0.002337 0.008278 0.026685 0.028261 0.007243 0.016020 0.037948 0.028840 0.003333 0.015453
Eisosomes 0.000040 0.000017 0.000007 0.000017 0.000018 0.000023 0.000027 0.000026 0.000024 0.000021 0.000011 0.000026
Endoplasmic Reticulum 0.003980 0.002408 0.001293 0.001329 0.002774 0.001606 0.003867 0.001255 0.003093 0.001811 0.001815 0.001260
Endosome 0.003490 0.001863 0.000365 0.005803 0.001926 0.002331 0.002068 0.005865 0.002340 0.002211 0.000711 0.005860
Golgi 0.000356 0.000701 0.000055 0.006140 0.000679 0.000800 0.000150 0.003687 0.000594 0.000775 0.000074 0.003867
Lipid Particles 0.001662 0.000760 0.000297 0.001219 0.001008 0.000846 0.001165 0.000799 0.001181 0.000824 0.000473 0.000830
Mitochondria 0.000716 0.000250 0.000642 0.000358 0.001394 0.001389 0.000421 0.001222 0.001215 0.001097 0.000597 0.001159
Mitotic Spindle 0.000995 0.006288 0.004169 0.066339 0.003796 0.006369 0.002419 0.029161 0.003055 0.006348 0.003813 0.031886
None 0.003613 0.006503 0.009252 0.010473 0.005332 0.007665 0.006710 0.009093 0.004877 0.007367 0.008736 0.009194
Nuclear Periphery 0.000430 0.000395 0.001094 0.001979 0.000616 0.000563 0.001131 0.000645 0.000567 0.000520 0.001101 0.000743
Nuclear Periphery Foci 0.003734 0.003320 0.000698 0.007201 0.002966 0.000820 0.004097 0.001893 0.003169 0.001460 0.001388 0.002282
Nucleolus 0.000550 0.000340 0.000145 0.000214 0.000534 0.000692 0.000230 0.000515 0.000538 0.000602 0.000163 0.000493
Nucleus 0.272174* 0.243945* 0.537910* 0.314700* 0.357781* 0.335173* 0.400211* 0.187497* 0.335127* 0.311799* 0.509956* 0.196819*
Peroxisomes 0.000439 0.000975 0.000051 0.000086 0.000507 0.001199 0.000123 0.000395 0.000489 0.001141 0.000066 0.000373
Vacuole 0.043438 0.033676 0.018412 0.199827 0.021320 0.043353 0.113061 0.097762 0.027173 0.040874 0.037626 0.105242
Vacuole Periphery 0.000543 0.000153 0.000067 0.061147 0.000481 0.000457 0.000195 0.003084 0.000498 0.000379 0.000093 0.007339

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.12 1.05 -4.82 0.47 -5.15 -0.70 -8.36 -4.77 -1.23 5.81 -1.22 -4.76 -5.83 0.67 -2.06
Bud Neck -1.30 -6.31 -6.22 -5.21 -2.85 -5.32 -6.48 -6.91 1.65 -0.40 -5.47 -7.51 -8.33 0.22 1.24
Bud Site -0.41 0.42 -2.47 -2.31 -2.58 -3.88 -4.65 -6.17 -0.51 -1.59 -3.84 -2.61 -6.09 -1.27 -3.58
Cell Periphery 5.15 7.53 -4.33 -6.92 -7.76 6.61 -2.82 -0.44 -2.20 0.61 8.35 5.96 -0.47 -2.54 -1.91
Cytoplasm -3.92 17.28 12.60 14.84 5.16 2.43 16.58 7.78 5.29 -9.52 0.38 26.83 10.54 9.92 -10.24
Cytoplasmic Foci 7.12 15.65 13.41 7.52 -4.92 -0.88 12.29 9.52 10.46 -4.15 3.75 20.77 15.41 12.54 -9.20
Eisosomes 6.17 9.48 -0.15 -5.94 -8.91 -3.35 -6.52 -5.29 -1.92 2.49 1.08 6.71 -2.37 -3.57 -9.37
Endoplasmic Reticulum 2.30 4.22 2.97 0.62 -1.83 3.26 -3.61 5.91 0.44 7.70 4.00 3.69 7.13 1.25 5.56
Endosome 2.81 6.45 2.73 0.35 -3.11 -1.00 -0.04 -0.72 -0.12 -0.74 0.51 7.03 0.10 -0.19 -3.09
Golgi -1.08 4.83 -1.09 -0.45 -1.64 -0.15 2.11 -1.09 -0.95 -2.63 -0.45 2.82 -1.41 -1.08 -3.00
Lipid Particles 3.55 5.81 -3.56 -6.23 -7.61 0.80 -2.83 -0.05 -1.34 4.46 2.16 3.53 0.16 -3.03 -6.99
Mitochondria 1.08 -0.32 0.08 -3.94 1.55 0.09 1.12 0.91 1.52 -0.78 0.28 0.80 0.87 1.04 0.33
Mitotic Spindle -1.92 -4.97 -1.63 -1.11 -1.11 -1.33 -0.23 -5.79 -4.86 -5.90 -2.04 -2.37 -6.35 -5.09 -5.62
None -2.49 -6.94 -1.25 0.38 1.96 -1.78 -1.18 -4.28 -1.04 -2.60 -2.51 -6.31 -5.15 -1.32 -0.20
Nuclear Periphery -0.19 -15.77 -6.45 -6.46 -2.69 -0.08 -9.30 -5.70 -9.42 5.71 -0.13 -12.78 -8.55 -12.18 4.73
Nuclear Periphery Foci 0.05 6.69 0.06 0.01 -4.41 6.19 -2.48 3.43 -5.60 5.85 5.21 5.53 4.34 -1.76 -1.93
Nucleolus 1.09 1.82 1.44 0.33 -2.11 -0.51 1.20 0.74 2.60 -1.52 -0.16 1.81 1.03 2.28 -2.14
Nucleus 1.61 -23.95 -2.26 -3.13 8.61 2.13 -5.87 8.56 6.23 11.89 2.51 -25.15 6.36 3.94 30.10
Peroxisomes -1.26 5.79 4.25 2.08 -5.73 -1.34 1.36 1.19 2.42 -0.61 -1.63 2.16 1.54 2.91 -2.10
Vacuole 0.98 2.69 -10.43 -10.63 -10.96 -10.27 -15.38 -23.58 -16.93 -1.03 -8.10 -11.36 -24.22 -19.67 -17.94
Vacuole Periphery 1.91 2.29 -1.30 -1.38 -1.40 0.21 1.49 -1.02 -1.21 -1.82 0.75 2.43 -1.61 -1.91 -2.41
Standard name
Human Ortholog
Description Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; IGO1 has a paralog, IGO2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (91%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Igo1

Igo1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Igo1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available