Standard name | |
---|---|
Human Ortholog | |
Description | Linker protein responsible for recruitment of myosin to the bud neck; interacts with the C-terminal extensions of septins Cdc11p and Shs1p and binds Myo1p to promote cytokinesis |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 4 | 14 | 2 | 2 | 6 | 2 | 4 | 1 | 6 | 8 | 1 | 2 | 8 | 5 | 10 | 2 | 4 | 3 | 3 | 5 | 7 | 0 | 1 | 0 |
Bud | 6 | 7 | 20 | 9 | 25 | 30 | 40 | 32 | 46 | 46 | 29 | 35 | 7 | 4 | 10 | 2 | 3 | 5 | 30 | 34 | 55 | 8 | 5 | 1 |
Bud Neck | 15 | 17 | 23 | 22 | 42 | 31 | 41 | 39 | 63 | 63 | 60 | 63 | 37 | 36 | 37 | 14 | 10 | 5 | 30 | 37 | 54 | 10 | 3 | 0 |
Bud Site | 5 | 5 | 11 | 9 | 9 | 8 | 5 | 7 | 22 | 21 | 12 | 15 | 10 | 2 | 11 | 3 | 5 | 1 | – | – | – | – | – | – |
Cell Periphery | 2 | 7 | 13 | 5 | 1 | 3 | 10 | 8 | 11 | 24 | 10 | 18 | 6 | 7 | 4 | 3 | 6 | 1 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 74 | 99 | 172 | 100 | 95 | 142 | 150 | 153 | 165 | 236 | 178 | 196 | 120 | 88 | 98 | 45 | 71 | 40 | 52 | 80 | 122 | 174 | 329 | 338 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 2 | 2 | 1 | 1 | 3 | 2 | 1 | 1 | 0 | 0 | 0 | 3 | 4 | 2 | 0 | 2 | 5 | 2 | 0 | 1 |
Endosome | 4 | 1 | 9 | 4 | 15 | 10 | 6 | 10 | 7 | 5 | 5 | 4 | 16 | 10 | 8 | 3 | 0 | 2 | 1 | 2 | 2 | 1 | 2 | 0 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 2 | 0 | 2 | 0 |
Mitochondria | 11 | 4 | 8 | 21 | 22 | 68 | 99 | 100 | 119 | 196 | 165 | 214 | 0 | 1 | 3 | 3 | 4 | 2 | 0 | 0 | 4 | 0 | 0 | 0 |
Nucleus | 1 | 0 | 1 | 2 | 4 | 6 | 8 | 11 | 13 | 16 | 9 | 4 | 7 | 4 | 11 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 2 | 0 | 0 | 2 | 4 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nucleolus | 7 | 4 | 3 | 6 | 16 | 13 | 22 | 21 | 31 | 36 | 20 | 30 | 9 | 5 | 19 | 6 | 5 | 5 | 1 | 1 | 0 | 0 | 0 | 0 |
Peroxisomes | 3 | 2 | 10 | 6 | 8 | 4 | 7 | 5 | 6 | 5 | 4 | 8 | 6 | 4 | 9 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2 | 0 | 24 | 12 | 21 | 24 | 18 | 33 | 48 | 41 | 20 | 44 | 23 | 38 | 43 | 3 | 2 | 2 | 5 | 3 | 13 | 1 | 3 | 0 |
Vac/Vac Membrane | 2 | 3 | 4 | 2 | 4 | 7 | 12 | 23 | 19 | 28 | 17 | 21 | 4 | 9 | 9 | 5 | 3 | 5 | 0 | 1 | 1 | 3 | 0 | 1 |
Unique Cell Count | 118 | 146 | 248 | 161 | 209 | 269 | 309 | 346 | 408 | 516 | 398 | 478 | 220 | 187 | 227 | 82 | 96 | 62 | 129 | 170 | 277 | 206 | 359 | 347 |
Labelled Cell Count | 137 | 163 | 301 | 201 | 272 | 350 | 425 | 446 | 559 | 727 | 533 | 659 | 253 | 214 | 274 | 97 | 120 | 74 | 129 | 170 | 277 | 206 | 359 | 347 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.5 | 10.6 | 8.4 | 7.6 | 7.4 | 6.4 | 6.0 | 5.9 | 5.9 | 5.5 | 5.1 | 5.1 | 10.3 | 10.5 | 9.7 | 14.5 | 14.4 | 14.0 | 10.0 | 10.3 | 10.4 |
Std Deviation (1e-4) | 1.3 | 1.7 | 1.2 | 1.8 | 2.5 | 1.7 | 1.9 | 1.6 | 1.9 | 1.2 | 1.3 | 1.2 | 1.8 | 2.0 | 2.1 | 3.2 | 2.8 | 3.5 | 1.4 | 1.6 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.15 | -0.17 | -0.39 | -0.47 | -0.5 | -0.5 | -0.6 | -0.71 | -0.71 | 0.3 | 0.32 | 0.21 | 0.79 | 0.78 | 0.73 | 0.26 | 0.3 | 0.31 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 0 | 0 | 0 |
Bud | -1.0 | 1.4 | 1.2 | 1.8 | 0.5 | 1.3 | 0.4 | -0.4 | -0.4 | -2.3 | -2.7 | -1.6 | -1.8 | -1.6 | 0 |
Bud Neck | 1.4 | 3.3 | 0.8 | 1.5 | 0.8 | 2.3 | 1.2 | 2.1 | 1.5 | 2.4 | 3.0 | 2.3 | 1.9 | 0.3 | -0.3 |
Bud Site | 0.5 | -0.1 | -0.9 | -2.0 | -1.7 | 0.5 | -0.2 | -0.9 | -0.9 | 0.1 | -2.0 | 0.2 | 0 | 0 | 0 |
Cell Periphery | -1.0 | -2.9 | -2.7 | -1.2 | -1.9 | -1.7 | -0.4 | -1.8 | -0.9 | -1.4 | -0.7 | -2.0 | 0 | 0.4 | 0 |
Cytoplasm | -1.5 | -5.2 | -3.9 | -4.9 | -6.1 | -7.2 | -6.1 | -6.1 | -7.2 | -3.3 | -4.7 | -5.8 | -2.4 | 0.8 | -0.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -0.6 | 1.7 | 0.1 | -1.2 | -0.5 | -1.5 | 0 | -2.0 | 0 | 1.7 | 0.9 | -0.1 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.8 | 3.1 | 7.1 | 8.6 | 8.0 | 8.2 | 10.2 | 10.7 | 11.5 | 0 | 0 | -1.4 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 2.4 | 2.4 | 2.4 | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 3.4 | 2.4 | 3.3 | 3.0 | 3.6 | 3.4 | 2.5 | 3.1 | 2.0 | 0 | 3.7 | 0 | 0 | 0 |
Peroxisomes | -0.2 | -0.1 | -1.8 | -1.2 | -2.0 | -2.1 | 0 | -2.6 | -1.9 | -0.8 | -1.1 | 0 | 0 | 0 | 0 |
SpindlePole | -0.8 | 0.1 | -0.3 | -1.7 | -0.1 | 0.8 | -0.8 | -2.3 | -0.2 | 0.3 | 3.1 | 2.9 | -1.7 | -2.4 | -1.6 |
Vacuole | 0 | 0 | 0.8 | 1.6 | 2.9 | 2.1 | 2.5 | 1.9 | 1.9 | 0 | 1.9 | 1.6 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.5544 | 5.1915 | 4.7282 | 4.3671 | 5.4885 | 4.4409 | 3.4296 | 4.9583 | 5.0393 | 3.5456 | 3.4765 | 4.4527 | 3.3585 | 4.8171 | 4.8267 | 4.3973 | 4.5016 | 4.3833 |
Actin | 0.0059 | 0.0029 | 0.0524 | 0.0292 | 0.0008 | 0.0013 | 0.0449 | 0.0145 | 0.0264 | 0.0259 | 0.0262 | 0.0125 | 0.0474 | 0.0048 | 0.0345 | 0.0014 | 0.0465 | 0.0052 |
Bud | 0.0065 | 0.03 | 0.0115 | 0.0003 | 0.0002 | 0.0058 | 0.0252 | 0.0303 | 0.0159 | 0.002 | 0.005 | 0.003 | 0.0274 | 0.0404 | 0.0105 | 0.0093 | 0.0035 | 0.018 |
Bud Neck | 0.0065 | 0.0426 | 0.4685 | 0.9369 | 0.6555 | 0.2347 | 0.0043 | 0.0245 | 0.4511 | 0.6143 | 0.5667 | 0.2853 | 0.0466 | 0.0217 | 0.5692 | 0.8293 | 0.6888 | 0.2441 |
Bud Periphery | 0.0033 | 0.0213 | 0.0125 | 0.0005 | 0.0001 | 0.0034 | 0.0174 | 0.0139 | 0.0177 | 0.0023 | 0.004 | 0.0019 | 0.0212 | 0.0273 | 0.0127 | 0.0133 | 0.0024 | 0.0207 |
Bud Site | 0.2116 | 0.4423 | 0.3236 | 0.0034 | 0.0025 | 0.01 | 0.1258 | 0.4393 | 0.3448 | 0.0986 | 0.0152 | 0.0108 | 0.3077 | 0.3902 | 0.2756 | 0.061 | 0.0283 | 0.0065 |
Cell Periphery | 0.0011 | 0.0052 | 0.0028 | 0.0005 | 0.0002 | 0.0004 | 0.0027 | 0.0019 | 0.0034 | 0.0015 | 0.0006 | 0.0003 | 0.0031 | 0.0025 | 0.0029 | 0.0016 | 0.0005 | 0.0009 |
Cytoplasm | 0.5691 | 0.3481 | 0.0327 | 0.0002 | 0.2815 | 0.6605 | 0.3633 | 0.3749 | 0.0631 | 0.0006 | 0.0746 | 0.5674 | 0.2867 | 0.4263 | 0.0142 | 0.0008 | 0.0833 | 0.5543 |
Cytoplasmic Foci | 0.0398 | 0.0193 | 0.0283 | 0.0028 | 0.0067 | 0.038 | 0.0816 | 0.0462 | 0.012 | 0.0081 | 0.1311 | 0.0339 | 0.0965 | 0.0267 | 0.0299 | 0.0691 | 0.0936 | 0.0487 |
Eisosomes | 0.0002 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0005 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0 | 0.0008 | 0 | 0.0003 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0322 | 0.0018 | 0.0049 | 0.0001 | 0.0048 | 0.0232 | 0.0176 | 0.003 | 0.0012 | 0.0007 | 0.0056 | 0.0108 | 0.0096 | 0.0021 | 0.0007 | 0 | 0.0001 | 0.0067 |
Endosome | 0.0221 | 0.0033 | 0.002 | 0.0018 | 0.001 | 0.0082 | 0.116 | 0.0055 | 0.0323 | 0.0264 | 0.0416 | 0.02 | 0.0243 | 0.0058 | 0.0056 | 0.0005 | 0.0021 | 0.0269 |
Golgi | 0.0043 | 0.0007 | 0.0051 | 0.0041 | 0.0004 | 0.0019 | 0.0271 | 0.0046 | 0.0048 | 0.0376 | 0.063 | 0.0141 | 0.0079 | 0.0008 | 0.005 | 0.0013 | 0.0046 | 0.0075 |
Lipid Particles | 0.0105 | 0.0005 | 0.0032 | 0.0101 | 0.0004 | 0.0002 | 0.0248 | 0.001 | 0.0009 | 0.0158 | 0.0049 | 0.0016 | 0.0099 | 0.0005 | 0.0176 | 0.0022 | 0.0051 | 0.0114 |
Mitochondria | 0.0047 | 0.0011 | 0.0353 | 0.0011 | 0.0001 | 0.0016 | 0.0613 | 0.0035 | 0.0014 | 0.1521 | 0.0261 | 0.0031 | 0.013 | 0.0006 | 0.0036 | 0.0002 | 0.0027 | 0.0047 |
None | 0.0417 | 0.0015 | 0.0009 | 0.0001 | 0.0423 | 0.0056 | 0.0125 | 0.0053 | 0.0034 | 0.0002 | 0.0004 | 0.0077 | 0.0205 | 0.0046 | 0.0008 | 0.0001 | 0.0003 | 0.0019 |
Nuclear Periphery | 0.0109 | 0.0019 | 0.0005 | 0.0002 | 0.0006 | 0.0004 | 0.0177 | 0.0005 | 0.0005 | 0.0006 | 0.0002 | 0.0024 | 0.0122 | 0.0013 | 0.0007 | 0 | 0.0001 | 0.0148 |
Nucleolus | 0.0037 | 0.0067 | 0.0012 | 0.001 | 0.0011 | 0.0001 | 0.0014 | 0.0084 | 0.0033 | 0.0026 | 0.0015 | 0.0003 | 0.0133 | 0.0037 | 0.0046 | 0.0014 | 0.0018 | 0.0032 |
Nucleus | 0.0043 | 0.0169 | 0.0007 | 0.0003 | 0.0005 | 0.0005 | 0.0045 | 0.0118 | 0.0027 | 0.0012 | 0.0005 | 0.0011 | 0.0159 | 0.0075 | 0.0012 | 0.0008 | 0.0005 | 0.0041 |
Peroxisomes | 0.0025 | 0.0005 | 0.0113 | 0.0061 | 0.0002 | 0.0009 | 0.0094 | 0.0025 | 0.0021 | 0.0045 | 0.0262 | 0.02 | 0.0165 | 0.001 | 0.0059 | 0.0045 | 0.0321 | 0.007 |
Punctate Nuclear | 0.0115 | 0.0018 | 0.0008 | 0.0009 | 0.0011 | 0.0004 | 0.0052 | 0.003 | 0.001 | 0.0009 | 0.0048 | 0.0014 | 0.0077 | 0.0038 | 0.0025 | 0.0011 | 0.0027 | 0.0053 |
Vacuole | 0.0062 | 0.047 | 0.0013 | 0.0002 | 0.0002 | 0.0024 | 0.0274 | 0.0051 | 0.011 | 0.0022 | 0.0011 | 0.0016 | 0.0092 | 0.0268 | 0.0018 | 0.002 | 0.0007 | 0.0068 |
Vacuole Periphery | 0.0014 | 0.0047 | 0.0003 | 0.0001 | 0 | 0.0004 | 0.0096 | 0.0004 | 0.001 | 0.0014 | 0.0004 | 0.0006 | 0.0025 | 0.0015 | 0.0003 | 0.0001 | 0.0001 | 0.0013 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 40.5483 | 30.0233 | 14.6713 | 14.314 | 31.3311 | 41.7539 | 27.58 | 18.4897 | 17.6075 | 35.842 |
Translational Efficiency | 3.2628 | 3.2285 | 1.9552 | 2.775 | 3.2365 | 2.5399 | 2.0314 | 2.227 | 2.0059 | 2.0304 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
81 | 503 | 650 | 200 | 1923 | 2384 | 1501 | 2511 | 2004 | 2887 | 2151 | 2711 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 892.95 | 976.63 | 1150.52 | 1198.55 | 904.41 | 972.84 | 1199.61 | 1329.85 | 903.95 | 973.50 | 1184.78 | 1320.16 |
Standard Deviation | 148.65 | 199.40 | 190.95 | 165.21 | 120.81 | 129.90 | 187.54 | 226.56 | 122.08 | 144.44 | 189.92 | 225.24 |
Intensity Change Log 2 | — | 0.129233 | 0.365635 | 0.424639 | — | 0.105226 | 0.407517 | 0.556215 | — | 0.117203 | 0.386861 | 0.492345 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000489 | 0.000534 | 0.000579 | 0.000487 | 0.000355 | 0.000399 | 0.000477 | 0.000344 | 0.000360 | 0.000423 | 0.000508 | 0.000354 |
Bud Neck | 0.135388 | 0.109792 | 0.136334 | 0.088393 | 0.158524 | 0.122995 | 0.117715 | 0.092855 | 0.157589 | 0.120695 | 0.123342 | 0.092526 |
Bud Site | 0.478702* | 0.422594* | 0.464824* | 0.417932* | 0.434053* | 0.442318* | 0.561900* | 0.514765* | 0.435857* | 0.438882* | 0.532565* | 0.507622* |
Cell Periphery | 0.001612 | 0.001834 | 0.000881 | 0.000563 | 0.002113 | 0.001218 | 0.000940 | 0.001164 | 0.002093 | 0.001325 | 0.000922 | 0.001120 |
Cytoplasm | 0.180269* | 0.287658* | 0.229180* | 0.355445* | 0.285948* | 0.316507* | 0.227837* | 0.330742* | 0.281677* | 0.311481* | 0.228242* | 0.332565* |
Cytoplasmic Foci | 0.101593 | 0.092696 | 0.061734 | 0.084375 | 0.071427 | 0.067458 | 0.032800 | 0.024075 | 0.072647 | 0.071856 | 0.041543 | 0.028523 |
Eisosomes | 0.000308 | 0.000221 | 0.000369 | 0.000576 | 0.000196 | 0.000156 | 0.000229 | 0.000188 | 0.000201 | 0.000167 | 0.000272 | 0.000217 |
Endoplasmic Reticulum | 0.001917 | 0.000659 | 0.000408 | 0.000066 | 0.001594 | 0.000277 | 0.000161 | 0.000081 | 0.001607 | 0.000343 | 0.000236 | 0.000080 |
Endosome | 0.014781 | 0.010060 | 0.004729 | 0.000910 | 0.006858 | 0.006515 | 0.002110 | 0.001484 | 0.007178 | 0.007133 | 0.002902 | 0.001442 |
Golgi | 0.008273 | 0.004607 | 0.001240 | 0.000309 | 0.004391 | 0.005841 | 0.000781 | 0.001919 | 0.004548 | 0.005626 | 0.000920 | 0.001800 |
Lipid Particles | 0.003652 | 0.000808 | 0.000516 | 0.000276 | 0.000666 | 0.000327 | 0.000176 | 0.000269 | 0.000786 | 0.000411 | 0.000279 | 0.000270 |
Mitochondria | 0.007720 | 0.001485 | 0.000331 | 0.000133 | 0.001504 | 0.001616 | 0.000529 | 0.002070 | 0.001755 | 0.001593 | 0.000469 | 0.001927 |
Mitotic Spindle | 0.006881 | 0.005738 | 0.002776 | 0.003191 | 0.003225 | 0.006824 | 0.007917 | 0.010340 | 0.003373 | 0.006635 | 0.006364 | 0.009812 |
None | 0.000547 | 0.003689 | 0.000496 | 0.000454 | 0.001704 | 0.000629 | 0.000412 | 0.000605 | 0.001657 | 0.001162 | 0.000437 | 0.000593 |
Nuclear Periphery | 0.000189 | 0.000103 | 0.000074 | 0.000021 | 0.000051 | 0.000032 | 0.000112 | 0.000040 | 0.000057 | 0.000044 | 0.000100 | 0.000038 |
Nuclear Periphery Foci | 0.004255 | 0.001263 | 0.001721 | 0.001419 | 0.001401 | 0.000651 | 0.000875 | 0.000259 | 0.001517 | 0.000758 | 0.001130 | 0.000345 |
Nucleolus | 0.000960 | 0.004184 | 0.000419 | 0.000069 | 0.000620 | 0.000545 | 0.000416 | 0.001481 | 0.000634 | 0.001179 | 0.000417 | 0.001377 |
Nucleus | 0.008367 | 0.009838 | 0.008499 | 0.003125 | 0.003978 | 0.002151 | 0.004050 | 0.003024 | 0.004155 | 0.003490 | 0.005395 | 0.003031 |
Peroxisomes | 0.002730 | 0.012371 | 0.006979 | 0.003460 | 0.003928 | 0.004163 | 0.005522 | 0.004270 | 0.003880 | 0.005593 | 0.005962 | 0.004210 |
Vacuole | 0.031977 | 0.028632 | 0.077531 | 0.038714 | 0.016235 | 0.017870 | 0.034668 | 0.009339 | 0.016872 | 0.019745 | 0.047621 | 0.011506 |
Vacuole Periphery | 0.009390 | 0.001233 | 0.000382 | 0.000081 | 0.001227 | 0.001507 | 0.000372 | 0.000686 | 0.001557 | 0.001460 | 0.000375 | 0.000641 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.26 | -0.41 | -0.14 | 0.17 | 0.38 | -0.59 | -2.05 | -0.32 | 0.43 | 2.32 | -0.87 | -2.30 | -0.41 | 0.70 | 2.57 |
Bud Neck | 1.13 | 0.52 | 2.53 | 2.79 | 4.12 | 5.57 | 8.48 | 12.13 | 7.19 | 3.40 | 6.14 | 7.87 | 12.50 | 7.49 | 5.21 |
Bud Site | 1.42 | 1.21 | 2.47 | 1.93 | 2.33 | -0.67 | -6.16 | -4.98 | -4.51 | 1.82 | -0.07 | -4.29 | -3.97 | -4.24 | 0.54 |
Cell Periphery | -0.29 | 1.83 | 2.60 | 2.45 | 2.15 | 4.56 | 5.92 | 4.39 | 0.51 | -1.39 | 3.76 | 6.39 | 4.73 | 1.38 | -1.42 |
Cytoplasm | -3.52 | -2.48 | -5.74 | -3.58 | -5.03 | -3.03 | 1.21 | -6.82 | -3.96 | -7.79 | -3.20 | 1.42 | -7.75 | -5.01 | -9.58 |
Cytoplasmic Foci | 0.44 | 2.33 | 0.40 | -0.02 | -2.98 | 0.96 | 10.42 | 13.40 | 13.28 | 3.29 | 0.12 | 8.40 | 12.30 | 13.42 | 4.51 |
Eisosomes | 0.85 | -0.27 | -1.10 | -1.85 | -1.06 | 1.90 | -0.42 | 0.54 | -0.62 | 0.82 | 1.54 | -1.84 | 0.04 | -0.96 | 1.30 |
Endoplasmic Reticulum | 1.15 | 1.39 | 1.69 | 3.79 | 4.21 | 3.09 | 3.36 | 3.57 | 3.54 | 2.48 | 3.07 | 3.34 | 3.73 | 4.94 | 4.57 |
Endosome | 1.40 | 3.23 | 4.44 | 5.98 | 6.17 | 0.47 | 8.63 | 10.52 | 9.13 | 2.17 | 0.07 | 7.88 | 11.33 | 10.72 | 4.99 |
Golgi | 1.29 | 2.58 | 2.92 | 4.49 | 2.38 | -1.92 | 11.89 | 4.58 | 4.85 | -2.28 | -1.58 | 11.08 | 5.29 | 5.34 | -1.86 |
Lipid Particles | 1.27 | 1.37 | 1.46 | 2.28 | 2.17 | 3.71 | 5.04 | 3.61 | 0.03 | -0.87 | 2.94 | 3.66 | 3.57 | 0.96 | 0.27 |
Mitochondria | 1.81 | 2.16 | 2.22 | 2.86 | 1.63 | -0.17 | 1.97 | -0.81 | -0.66 | -4.59 | 0.27 | 2.60 | -0.14 | -0.52 | -4.74 |
Mitotic Spindle | 0.04 | 0.64 | 0.78 | 1.44 | 0.49 | -2.79 | -4.40 | -5.39 | -2.41 | -0.41 | -2.86 | -3.91 | -5.13 | -2.28 | -1.40 |
None | -1.59 | 0.17 | 0.38 | 1.67 | 0.25 | 3.50 | 4.56 | 3.86 | -0.06 | -2.00 | 1.15 | 4.46 | 3.91 | 1.46 | -1.59 |
Nuclear Periphery | 0.82 | 1.03 | 1.63 | 2.03 | 4.92 | 1.26 | -1.39 | 0.19 | -2.15 | 1.48 | 0.73 | -1.49 | 0.66 | -0.20 | 1.83 |
Nuclear Periphery Foci | 1.02 | 0.79 | 0.85 | -1.52 | 0.55 | 2.17 | 1.09 | 3.16 | 4.47 | 4.15 | 2.16 | 0.70 | 3.13 | 4.42 | 6.29 |
Nucleolus | -1.89 | 0.66 | 1.10 | 2.71 | 2.89 | 0.34 | 1.35 | -2.78 | -2.77 | -3.36 | -1.76 | 1.59 | -2.58 | -0.57 | -3.32 |
Nucleus | -0.27 | -0.19 | 0.71 | 2.10 | 2.95 | 3.62 | -3.13 | 0.16 | -4.64 | 3.64 | 1.00 | -3.91 | 0.38 | -0.83 | 4.84 |
Peroxisomes | -4.07 | -2.55 | -0.36 | 3.94 | 2.35 | -0.30 | -1.46 | -0.43 | -0.12 | 1.29 | -2.15 | -2.06 | -0.39 | 2.15 | 2.06 |
Vacuole | 0.12 | -5.36 | -2.12 | -3.21 | 3.58 | -1.37 | -10.00 | 2.40 | 3.66 | 10.88 | -2.36 | -13.97 | 0.28 | 2.57 | 14.02 |
Vacuole Periphery | 2.48 | 2.74 | 2.83 | 5.74 | 4.88 | -0.89 | 6.03 | 2.92 | 2.57 | -2.17 | 0.34 | 6.27 | 4.13 | 3.00 | -2.08 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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