Standard name
Human Ortholog
Description Linker protein responsible for recruitment of myosin to the bud neck; interacts with the C-terminal extensions of septins Cdc11p and Shs1p and binds Myo1p to promote cytokinesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Bud 0.05 0.05 0.08 0.06 0.12 0.11 0.13 0.09 0.11 0.09 0.07 0.07 0 0 0 0 0 0.08 0.24 0.2 0.2 0 0 0
Bud Neck 0.13 0.12 0.09 0.14 0.2 0.12 0.13 0.11 0.15 0.12 0.15 0.13 0.17 0.19 0.16 0.17 0.1 0.08 0.23 0.22 0.2 0.05 0 0
Bud Site 0 0 0 0.06 0 0 0 0 0.05 0 0 0 0.05 0 0.05 0 0.05 0 – – – – – –
Cell Periphery 0 0.05 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Cytoplasm 0.63 0.68 0.69 0.62 0.45 0.53 0.49 0.44 0.4 0.46 0.45 0.41 0.55 0.47 0.43 0.55 0.74 0.65 0.4 0.47 0.44 0.85 0.92 0.98
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.07 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0 0 0.13 0.11 0.25 0.32 0.29 0.29 0.38 0.41 0.45 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 0 0 0.08 0.05 0.07 0.06 0.08 0.07 0.05 0.06 0 0 0.08 0.07 0.05 0.08 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.1 0.07 0.1 0.09 0.06 0.1 0.12 0.08 0.05 0.09 0.1 0.2 0.19 0 0 0 0 0 0.05 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.07 0.05 0.05 0 0 0 0.05 0 0.06 0 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 14 2 2 6 2 4 1 6 8 1 2 8 5 10 2 4 3 3 5 7 0 1 0
Bud 6 7 20 9 25 30 40 32 46 46 29 35 7 4 10 2 3 5 30 34 55 8 5 1
Bud Neck 15 17 23 22 42 31 41 39 63 63 60 63 37 36 37 14 10 5 30 37 54 10 3 0
Bud Site 5 5 11 9 9 8 5 7 22 21 12 15 10 2 11 3 5 1 – – – – – –
Cell Periphery 2 7 13 5 1 3 10 8 11 24 10 18 6 7 4 3 6 1 0 0 1 0 0 0
Cytoplasm 74 99 172 100 95 142 150 153 165 236 178 196 120 88 98 45 71 40 52 80 122 174 329 338
Endoplasmic Reticulum 1 0 0 0 2 2 1 1 3 2 1 1 0 0 0 3 4 2 0 2 5 2 0 1
Endosome 4 1 9 4 15 10 6 10 7 5 5 4 16 10 8 3 0 2 1 2 2 1 2 0
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 2 0 0 0 2 0 2 0
Mitochondria 11 4 8 21 22 68 99 100 119 196 165 214 0 1 3 3 4 2 0 0 4 0 0 0
Nucleus 1 0 1 2 4 6 8 11 13 16 9 4 7 4 11 1 0 0 0 0 0 0 1 1
Nuclear Periphery 0 0 0 1 2 0 2 2 0 0 2 4 0 1 2 0 0 0 0 0 0 1 0 0
Nucleolus 7 4 3 6 16 13 22 21 31 36 20 30 9 5 19 6 5 5 1 1 0 0 0 0
Peroxisomes 3 2 10 6 8 4 7 5 6 5 4 8 6 4 9 2 1 1 0 0 0 0 0 0
SpindlePole 2 0 24 12 21 24 18 33 48 41 20 44 23 38 43 3 2 2 5 3 13 1 3 0
Vac/Vac Membrane 2 3 4 2 4 7 12 23 19 28 17 21 4 9 9 5 3 5 0 1 1 3 0 1
Unique Cell Count 118 146 248 161 209 269 309 346 408 516 398 478 220 187 227 82 96 62 129 170 277 206 359 347
Labelled Cell Count 137 163 301 201 272 350 425 446 559 727 533 659 253 214 274 97 120 74 129 170 277 206 359 347


Ambiguous, Bud, Bud Neck, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.5 10.6 8.4 7.6 7.4 6.4 6.0 5.9 5.9 5.5 5.1 5.1 10.3 10.5 9.7 14.5 14.4 14.0 10.0 10.3 10.4
Std Deviation (1e-4) 1.3 1.7 1.2 1.8 2.5 1.7 1.9 1.6 1.9 1.2 1.3 1.2 1.8 2.0 2.1 3.2 2.8 3.5 1.4 1.6 1.4
Intensity Change (Log2) – – – -0.15 -0.17 -0.39 -0.47 -0.5 -0.5 -0.6 -0.71 -0.71 0.3 0.32 0.21 0.79 0.78 0.73 0.26 0.3 0.31

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP700051015WT3HU80HU120HU160051015WT3rpd3Δ_1rpd3Δ_2rpd3Δ_3051015WT1AF100AF140AF180051015
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 2.5 0 0 0
Bud -1.0 1.4 1.2 1.8 0.5 1.3 0.4 -0.4 -0.4 -2.3 -2.7 -1.6 -1.8 -1.6 0
Bud Neck 1.4 3.3 0.8 1.5 0.8 2.3 1.2 2.1 1.5 2.4 3.0 2.3 1.9 0.3 -0.3
Bud Site 0.5 -0.1 -0.9 -2.0 -1.7 0.5 -0.2 -0.9 -0.9 0.1 -2.0 0.2 0 0 0
Cell Periphery -1.0 -2.9 -2.7 -1.2 -1.9 -1.7 -0.4 -1.8 -0.9 -1.4 -0.7 -2.0 0 0.4 0
Cytoplasm -1.5 -5.2 -3.9 -4.9 -6.1 -7.2 -6.1 -6.1 -7.2 -3.3 -4.7 -5.8 -2.4 0.8 -0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -0.6 1.7 0.1 -1.2 -0.5 -1.5 0 -2.0 0 1.7 0.9 -0.1 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.8 3.1 7.1 8.6 8.0 8.2 10.2 10.7 11.5 0 0 -1.4 0 0 0
Nucleus 0 0 0 0 2.4 2.4 2.4 0 0 0 0 3.1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 3.4 2.4 3.3 3.0 3.6 3.4 2.5 3.1 2.0 0 3.7 0 0 0
Peroxisomes -0.2 -0.1 -1.8 -1.2 -2.0 -2.1 0 -2.6 -1.9 -0.8 -1.1 0 0 0 0
SpindlePole -0.8 0.1 -0.3 -1.7 -0.1 0.8 -0.8 -2.3 -0.2 0.3 3.1 2.9 -1.7 -2.4 -1.6
Vacuole 0 0 0.8 1.6 2.9 2.1 2.5 1.9 1.9 0 1.9 1.6 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.5544 5.1915 4.7282 4.3671 5.4885 4.4409 3.4296 4.9583 5.0393 3.5456 3.4765 4.4527 3.3585 4.8171 4.8267 4.3973 4.5016 4.3833
Actin 0.0059 0.0029 0.0524 0.0292 0.0008 0.0013 0.0449 0.0145 0.0264 0.0259 0.0262 0.0125 0.0474 0.0048 0.0345 0.0014 0.0465 0.0052
Bud 0.0065 0.03 0.0115 0.0003 0.0002 0.0058 0.0252 0.0303 0.0159 0.002 0.005 0.003 0.0274 0.0404 0.0105 0.0093 0.0035 0.018
Bud Neck 0.0065 0.0426 0.4685 0.9369 0.6555 0.2347 0.0043 0.0245 0.4511 0.6143 0.5667 0.2853 0.0466 0.0217 0.5692 0.8293 0.6888 0.2441
Bud Periphery 0.0033 0.0213 0.0125 0.0005 0.0001 0.0034 0.0174 0.0139 0.0177 0.0023 0.004 0.0019 0.0212 0.0273 0.0127 0.0133 0.0024 0.0207
Bud Site 0.2116 0.4423 0.3236 0.0034 0.0025 0.01 0.1258 0.4393 0.3448 0.0986 0.0152 0.0108 0.3077 0.3902 0.2756 0.061 0.0283 0.0065
Cell Periphery 0.0011 0.0052 0.0028 0.0005 0.0002 0.0004 0.0027 0.0019 0.0034 0.0015 0.0006 0.0003 0.0031 0.0025 0.0029 0.0016 0.0005 0.0009
Cytoplasm 0.5691 0.3481 0.0327 0.0002 0.2815 0.6605 0.3633 0.3749 0.0631 0.0006 0.0746 0.5674 0.2867 0.4263 0.0142 0.0008 0.0833 0.5543
Cytoplasmic Foci 0.0398 0.0193 0.0283 0.0028 0.0067 0.038 0.0816 0.0462 0.012 0.0081 0.1311 0.0339 0.0965 0.0267 0.0299 0.0691 0.0936 0.0487
Eisosomes 0.0002 0 0.0002 0.0001 0 0 0.0005 0.0001 0.0001 0.0004 0.0001 0 0.0008 0 0.0003 0 0.0001 0
Endoplasmic Reticulum 0.0322 0.0018 0.0049 0.0001 0.0048 0.0232 0.0176 0.003 0.0012 0.0007 0.0056 0.0108 0.0096 0.0021 0.0007 0 0.0001 0.0067
Endosome 0.0221 0.0033 0.002 0.0018 0.001 0.0082 0.116 0.0055 0.0323 0.0264 0.0416 0.02 0.0243 0.0058 0.0056 0.0005 0.0021 0.0269
Golgi 0.0043 0.0007 0.0051 0.0041 0.0004 0.0019 0.0271 0.0046 0.0048 0.0376 0.063 0.0141 0.0079 0.0008 0.005 0.0013 0.0046 0.0075
Lipid Particles 0.0105 0.0005 0.0032 0.0101 0.0004 0.0002 0.0248 0.001 0.0009 0.0158 0.0049 0.0016 0.0099 0.0005 0.0176 0.0022 0.0051 0.0114
Mitochondria 0.0047 0.0011 0.0353 0.0011 0.0001 0.0016 0.0613 0.0035 0.0014 0.1521 0.0261 0.0031 0.013 0.0006 0.0036 0.0002 0.0027 0.0047
None 0.0417 0.0015 0.0009 0.0001 0.0423 0.0056 0.0125 0.0053 0.0034 0.0002 0.0004 0.0077 0.0205 0.0046 0.0008 0.0001 0.0003 0.0019
Nuclear Periphery 0.0109 0.0019 0.0005 0.0002 0.0006 0.0004 0.0177 0.0005 0.0005 0.0006 0.0002 0.0024 0.0122 0.0013 0.0007 0 0.0001 0.0148
Nucleolus 0.0037 0.0067 0.0012 0.001 0.0011 0.0001 0.0014 0.0084 0.0033 0.0026 0.0015 0.0003 0.0133 0.0037 0.0046 0.0014 0.0018 0.0032
Nucleus 0.0043 0.0169 0.0007 0.0003 0.0005 0.0005 0.0045 0.0118 0.0027 0.0012 0.0005 0.0011 0.0159 0.0075 0.0012 0.0008 0.0005 0.0041
Peroxisomes 0.0025 0.0005 0.0113 0.0061 0.0002 0.0009 0.0094 0.0025 0.0021 0.0045 0.0262 0.02 0.0165 0.001 0.0059 0.0045 0.0321 0.007
Punctate Nuclear 0.0115 0.0018 0.0008 0.0009 0.0011 0.0004 0.0052 0.003 0.001 0.0009 0.0048 0.0014 0.0077 0.0038 0.0025 0.0011 0.0027 0.0053
Vacuole 0.0062 0.047 0.0013 0.0002 0.0002 0.0024 0.0274 0.0051 0.011 0.0022 0.0011 0.0016 0.0092 0.0268 0.0018 0.002 0.0007 0.0068
Vacuole Periphery 0.0014 0.0047 0.0003 0.0001 0 0.0004 0.0096 0.0004 0.001 0.0014 0.0004 0.0006 0.0025 0.0015 0.0003 0.0001 0.0001 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 40.5483 30.0233 14.6713 14.314 31.3311 41.7539 27.58 18.4897 17.6075 35.842
Translational Efficiency 3.2628 3.2285 1.9552 2.775 3.2365 2.5399 2.0314 2.227 2.0059 2.0304

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
81 503 650 200 1923 2384 1501 2511 2004 2887 2151 2711

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 892.95 976.63 1150.52 1198.55 904.41 972.84 1199.61 1329.85 903.95 973.50 1184.78 1320.16
Standard Deviation 148.65 199.40 190.95 165.21 120.81 129.90 187.54 226.56 122.08 144.44 189.92 225.24
Intensity Change Log 2 — 0.129233 0.365635 0.424639 — 0.105226 0.407517 0.556215 — 0.117203 0.386861 0.492345

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000489 0.000534 0.000579 0.000487 0.000355 0.000399 0.000477 0.000344 0.000360 0.000423 0.000508 0.000354
Bud Neck 0.135388 0.109792 0.136334 0.088393 0.158524 0.122995 0.117715 0.092855 0.157589 0.120695 0.123342 0.092526
Bud Site 0.478702 0.422594 0.464824 0.417932 0.434053 0.442318 0.561900 0.514765 0.435857 0.438882 0.532565 0.507622
Cell Periphery 0.001612 0.001834 0.000881 0.000563 0.002113 0.001218 0.000940 0.001164 0.002093 0.001325 0.000922 0.001120
Cytoplasm 0.180269 0.287658 0.229180 0.355445 0.285948 0.316507 0.227837 0.330742 0.281677 0.311481 0.228242 0.332565
Cytoplasmic Foci 0.101593 0.092696 0.061734 0.084375 0.071427 0.067458 0.032800 0.024075 0.072647 0.071856 0.041543 0.028523
Eisosomes 0.000308 0.000221 0.000369 0.000576 0.000196 0.000156 0.000229 0.000188 0.000201 0.000167 0.000272 0.000217
Endoplasmic Reticulum 0.001917 0.000659 0.000408 0.000066 0.001594 0.000277 0.000161 0.000081 0.001607 0.000343 0.000236 0.000080
Endosome 0.014781 0.010060 0.004729 0.000910 0.006858 0.006515 0.002110 0.001484 0.007178 0.007133 0.002902 0.001442
Golgi 0.008273 0.004607 0.001240 0.000309 0.004391 0.005841 0.000781 0.001919 0.004548 0.005626 0.000920 0.001800
Lipid Particles 0.003652 0.000808 0.000516 0.000276 0.000666 0.000327 0.000176 0.000269 0.000786 0.000411 0.000279 0.000270
Mitochondria 0.007720 0.001485 0.000331 0.000133 0.001504 0.001616 0.000529 0.002070 0.001755 0.001593 0.000469 0.001927
Mitotic Spindle 0.006881 0.005738 0.002776 0.003191 0.003225 0.006824 0.007917 0.010340 0.003373 0.006635 0.006364 0.009812
None 0.000547 0.003689 0.000496 0.000454 0.001704 0.000629 0.000412 0.000605 0.001657 0.001162 0.000437 0.000593
Nuclear Periphery 0.000189 0.000103 0.000074 0.000021 0.000051 0.000032 0.000112 0.000040 0.000057 0.000044 0.000100 0.000038
Nuclear Periphery Foci 0.004255 0.001263 0.001721 0.001419 0.001401 0.000651 0.000875 0.000259 0.001517 0.000758 0.001130 0.000345
Nucleolus 0.000960 0.004184 0.000419 0.000069 0.000620 0.000545 0.000416 0.001481 0.000634 0.001179 0.000417 0.001377
Nucleus 0.008367 0.009838 0.008499 0.003125 0.003978 0.002151 0.004050 0.003024 0.004155 0.003490 0.005395 0.003031
Peroxisomes 0.002730 0.012371 0.006979 0.003460 0.003928 0.004163 0.005522 0.004270 0.003880 0.005593 0.005962 0.004210
Vacuole 0.031977 0.028632 0.077531 0.038714 0.016235 0.017870 0.034668 0.009339 0.016872 0.019745 0.047621 0.011506
Vacuole Periphery 0.009390 0.001233 0.000382 0.000081 0.001227 0.001507 0.000372 0.000686 0.001557 0.001460 0.000375 0.000641

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.26 -0.41 -0.14 0.17 0.38 -0.59 -2.05 -0.32 0.43 2.32 -0.87 -2.30 -0.41 0.70 2.57
Bud Neck 1.13 0.52 2.53 2.79 4.12 5.57 8.48 12.13 7.19 3.40 6.14 7.87 12.50 7.49 5.21
Bud Site 1.42 1.21 2.47 1.93 2.33 -0.67 -6.16 -4.98 -4.51 1.82 -0.07 -4.29 -3.97 -4.24 0.54
Cell Periphery -0.29 1.83 2.60 2.45 2.15 4.56 5.92 4.39 0.51 -1.39 3.76 6.39 4.73 1.38 -1.42
Cytoplasm -3.52 -2.48 -5.74 -3.58 -5.03 -3.03 1.21 -6.82 -3.96 -7.79 -3.20 1.42 -7.75 -5.01 -9.58
Cytoplasmic Foci 0.44 2.33 0.40 -0.02 -2.98 0.96 10.42 13.40 13.28 3.29 0.12 8.40 12.30 13.42 4.51
Eisosomes 0.85 -0.27 -1.10 -1.85 -1.06 1.90 -0.42 0.54 -0.62 0.82 1.54 -1.84 0.04 -0.96 1.30
Endoplasmic Reticulum 1.15 1.39 1.69 3.79 4.21 3.09 3.36 3.57 3.54 2.48 3.07 3.34 3.73 4.94 4.57
Endosome 1.40 3.23 4.44 5.98 6.17 0.47 8.63 10.52 9.13 2.17 0.07 7.88 11.33 10.72 4.99
Golgi 1.29 2.58 2.92 4.49 2.38 -1.92 11.89 4.58 4.85 -2.28 -1.58 11.08 5.29 5.34 -1.86
Lipid Particles 1.27 1.37 1.46 2.28 2.17 3.71 5.04 3.61 0.03 -0.87 2.94 3.66 3.57 0.96 0.27
Mitochondria 1.81 2.16 2.22 2.86 1.63 -0.17 1.97 -0.81 -0.66 -4.59 0.27 2.60 -0.14 -0.52 -4.74
Mitotic Spindle 0.04 0.64 0.78 1.44 0.49 -2.79 -4.40 -5.39 -2.41 -0.41 -2.86 -3.91 -5.13 -2.28 -1.40
None -1.59 0.17 0.38 1.67 0.25 3.50 4.56 3.86 -0.06 -2.00 1.15 4.46 3.91 1.46 -1.59
Nuclear Periphery 0.82 1.03 1.63 2.03 4.92 1.26 -1.39 0.19 -2.15 1.48 0.73 -1.49 0.66 -0.20 1.83
Nuclear Periphery Foci 1.02 0.79 0.85 -1.52 0.55 2.17 1.09 3.16 4.47 4.15 2.16 0.70 3.13 4.42 6.29
Nucleolus -1.89 0.66 1.10 2.71 2.89 0.34 1.35 -2.78 -2.77 -3.36 -1.76 1.59 -2.58 -0.57 -3.32
Nucleus -0.27 -0.19 0.71 2.10 2.95 3.62 -3.13 0.16 -4.64 3.64 1.00 -3.91 0.38 -0.83 4.84
Peroxisomes -4.07 -2.55 -0.36 3.94 2.35 -0.30 -1.46 -0.43 -0.12 1.29 -2.15 -2.06 -0.39 2.15 2.06
Vacuole 0.12 -5.36 -2.12 -3.21 3.58 -1.37 -10.00 2.40 3.66 10.88 -2.36 -13.97 0.28 2.57 14.02
Vacuole Periphery 2.48 2.74 2.83 5.74 4.88 -0.89 6.03 2.92 2.57 -2.17 0.34 6.27 4.13 3.00 -2.08
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Linker protein responsible for recruitment of myosin to the bud neck; interacts with the C-terminal extensions of septins Cdc11p and Shs1p and binds Myo1p to promote cytokinesis
Localization
Cell Percentages cytoplasm (39%), bud neck (30%), mixed (21%)
Cell Cycle Regulation Yes
cytoplasm - T/G1 (3.7e-16)
bud neck - S/G2 (2.2e-09)
bud neck - MA (2.2e-11)
Subcompartmental Group N/A

Bni5

Bni5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bni5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available