Standard name | |
---|---|
Human Ortholog | |
Description | Basic leucine zipper transcription factor of the ATF/CREB family; forms a complex with Tup1p and Cyc8p to both activate and repress transcription; cytosolic and nuclear protein involved in osmotic and oxidative stress responses |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
Bud | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 2 | 0 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 4 | 2 | 2 | 1 | 0 |
Bud Neck | 1 | 0 | 1 | 1 | 0 | 1 | 1 | 3 | 1 | 4 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 3 | 1 | 6 | 4 | 9 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 4 | 7 | 14 | 18 | 11 | 21 | 14 | 3 | 9 | 5 | 6 | 12 | 28 | 32 | 35 | 31 | 21 | 0 | 3 | 1 | 3 | 2 | 1 |
Endoplasmic Reticulum | 1 | 1 | 0 | 1 | 0 | 1 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 8 | 11 | 8 | 0 | 0 | 0 | 0 | 1 | 0 |
Endosome | 1 | 5 | 1 | 5 | 1 | 1 | 2 | 3 | 0 | 2 | 0 | 1 | 0 | 2 | 0 | 6 | 12 | 10 | 2 | 6 | 7 | 0 | 1 | 2 |
Golgi | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 1 |
Mitochondria | 26 | 5 | 3 | 11 | 4 | 36 | 33 | 38 | 38 | 52 | 91 | 83 | 0 | 0 | 1 | 28 | 12 | 15 | 3 | 2 | 2 | 0 | 2 | 4 |
Nucleus | 136 | 268 | 169 | 110 | 125 | 148 | 170 | 154 | 56 | 121 | 112 | 148 | 111 | 167 | 88 | 288 | 222 | 254 | 116 | 250 | 150 | 82 | 93 | 114 |
Nuclear Periphery | 0 | 0 | 0 | 3 | 1 | 1 | 3 | 3 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 6 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 6 | 11 | 2 | 5 | 1 | 8 | 8 | 7 | 12 | 19 | 21 | 22 | 0 | 3 | 1 | 11 | 16 | 10 | 24 | 45 | 19 | 9 | 18 | 25 |
Peroxisomes | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 7 | 8 | 11 | 8 | 8 | 5 | 16 | 11 | 0 | 2 | 3 | 7 | 4 | 5 | 1 | 7 | 8 | 10 | 7 | 8 | 6 | 3 | 6 | 7 |
Vac/Vac Membrane | 2 | 7 | 6 | 3 | 1 | 9 | 1 | 1 | 3 | 1 | 0 | 1 | 1 | 0 | 4 | 17 | 13 | 18 | 10 | 12 | 20 | 10 | 12 | 14 |
Unique Cell Count | 155 | 291 | 184 | 141 | 150 | 191 | 214 | 203 | 82 | 166 | 163 | 202 | 122 | 199 | 125 | 357 | 292 | 315 | 172 | 340 | 215 | 114 | 143 | 178 |
Labelled Cell Count | 184 | 311 | 200 | 163 | 159 | 222 | 264 | 240 | 114 | 220 | 239 | 281 | 129 | 205 | 128 | 408 | 333 | 348 | 172 | 340 | 215 | 114 | 143 | 178 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.6 | 5.7 | 6.1 | 6.1 | 6.5 | 5.1 | 5.1 | 5.3 | 4.3 | 4.9 | 4.3 | 4.2 | 6.2 | 6.2 | 6.3 | 7.3 | 8.1 | 7.3 | 5.2 | 6.0 | 6.1 |
Std Deviation (1e-4) | 0.9 | 1.0 | 1.2 | 1.9 | 2.1 | 1.3 | 1.5 | 1.4 | 1.1 | 1.7 | 1.3 | 0.9 | 1.0 | 1.2 | 1.7 | 1.7 | 2.1 | 1.9 | 0.7 | 1.4 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.0 | 0.1 | -0.24 | -0.25 | -0.19 | -0.49 | -0.31 | -0.48 | -0.53 | 0.03 | 0.04 | 0.06 | 0.27 | 0.42 | 0.27 | -0.22 | -0.03 | 0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.2 | 2.8 | 0.9 | 2.3 | 1.3 | 0 | 0.7 | -0.4 | -0.5 | 2.1 | 3.5 | 5.7 | 2.5 | 2.7 | 1.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.7 | 0 | 5.5 | 4.8 | 5.5 | 0 | 7.6 | 0 | 0 | 0 | 0 | 0 | 2.9 | 1.5 | 1.8 |
Nucleus | -3.5 | -2.4 | -3.8 | -3.5 | -4.2 | -4.9 | -4.7 | -5.5 | -4.8 | -0.3 | -2.4 | -4.9 | -3.4 | -4.4 | -3.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 4.1 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -0.1 | -0.3 | -1.6 | 0.6 | -0.2 | 0 | -2.4 | 0 | 0 | -1.1 | -1.7 | 0 | -2.5 | -1.8 | -1.5 |
Vacuole | 0 | 0 | 0.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.8 | 0.6 | 1.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.7603 | 0.8488 | 0.7653 | 0.3123 | 0.2613 | 0.5126 | 1.0215 | 0.8342 | 0.8991 | 1.1479 | 1.0464 | 0.672 | 1.833 | 1.1378 | 0.8389 | 1.8038 | 1.2887 | 1.2143 |
Actin | 0.0048 | 0.0001 | 0.0075 | 0.0026 | 0.0058 | 0.0014 | 0.0143 | 0.0008 | 0.0011 | 0.003 | 0.0178 | 0.0001 | 0.0347 | 0 | 0.015 | 0.0663 | 0.0122 | 0.0059 |
Bud | 0.0005 | 0.0011 | 0.0003 | 0.0002 | 0.0014 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0003 | 0.0026 | 0.0001 | 0.0009 | 0 | 0.0001 | 0.0006 | 0.002 | 0.0001 |
Bud Neck | 0.0034 | 0.0005 | 0.0007 | 0.0002 | 0.0012 | 0.002 | 0.0023 | 0.0001 | 0.0003 | 0.0017 | 0.001 | 0.0009 | 0.0045 | 0.0001 | 0.0005 | 0.0011 | 0.0047 | 0.0012 |
Bud Periphery | 0.0017 | 0.0002 | 0.0004 | 0.0004 | 0.0045 | 0.0002 | 0.0005 | 0.0002 | 0.0005 | 0.0004 | 0.0055 | 0.0002 | 0.0023 | 0 | 0.0002 | 0.0011 | 0.006 | 0.0002 |
Bud Site | 0.0028 | 0.0011 | 0.0015 | 0.0006 | 0.0035 | 0.0003 | 0.0058 | 0.0003 | 0.0016 | 0.0016 | 0.0067 | 0.0001 | 0.0119 | 0.0001 | 0.0004 | 0.0021 | 0.0042 | 0.0001 |
Cell Periphery | 0.0003 | 0 | 0.0002 | 0 | 0.0013 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0006 | 0 | 0 | 0.0002 | 0.0015 | 0 |
Cytoplasm | 0.0074 | 0.0009 | 0.0028 | 0.0017 | 0.0013 | 0.0023 | 0.0013 | 0.0004 | 0.0014 | 0.0079 | 0.0008 | 0.0003 | 0.0047 | 0.0004 | 0.0002 | 0.0004 | 0.0089 | 0.0025 |
Cytoplasmic Foci | 0.0279 | 0.0001 | 0.0142 | 0.0073 | 0.0122 | 0.0059 | 0.0161 | 0.0003 | 0.0015 | 0.0154 | 0.0063 | 0.0001 | 0.0087 | 0 | 0.0092 | 0.0016 | 0.0134 | 0.0005 |
Eisosomes | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0004 | 0 | 0 | 0.0006 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.002 | 0 | 0.0034 | 0.0002 | 0.0007 | 0.0004 | 0.0026 | 0.0001 | 0.0011 | 0.0036 | 0.0013 | 0.0001 | 0.0049 | 0 | 0.0001 | 0.0007 | 0.0046 | 0.0038 |
Endosome | 0.0303 | 0 | 0.01 | 0.0092 | 0.0797 | 0.0119 | 0.0168 | 0.001 | 0.0037 | 0.0271 | 0.0574 | 0.0003 | 0.0119 | 0 | 0.0017 | 0.0056 | 0.0339 | 0.0067 |
Golgi | 0.0099 | 0 | 0.0064 | 0.0142 | 0.0178 | 0.0042 | 0.0066 | 0.0006 | 0.0009 | 0.0038 | 0.0373 | 0 | 0.0037 | 0 | 0.0023 | 0.0086 | 0.0192 | 0.0011 |
Lipid Particles | 0.0354 | 0 | 0.0048 | 0.0035 | 0.0504 | 0.0115 | 0.0168 | 0.0007 | 0.0093 | 0.0199 | 0.0091 | 0 | 0.0036 | 0 | 0.0081 | 0.0059 | 0.0488 | 0.0016 |
Mitochondria | 0.005 | 0.0001 | 0.0173 | 0.027 | 0.0257 | 0.0046 | 0.0038 | 0.0081 | 0.0032 | 0.0022 | 0.0167 | 0.0003 | 0.0061 | 0.0002 | 0.0045 | 0.0772 | 0.0526 | 0.0011 |
None | 0.0013 | 0.0002 | 0.0027 | 0.0027 | 0.0005 | 0.0013 | 0.0018 | 0.0008 | 0.0005 | 0.0495 | 0.0008 | 0.0002 | 0.004 | 0.0011 | 0.0003 | 0.0005 | 0.0167 | 0.0015 |
Nuclear Periphery | 0.0066 | 0.006 | 0.0066 | 0.0094 | 0.0065 | 0.0036 | 0.0107 | 0.0042 | 0.0071 | 0.0457 | 0.0047 | 0.0026 | 0.0222 | 0.001 | 0.001 | 0.003 | 0.0241 | 0.0203 |
Nucleolus | 0.0404 | 0.0327 | 0.0257 | 0.0213 | 0.0816 | 0.09 | 0.0199 | 0.0232 | 0.0139 | 0.0188 | 0.027 | 0.0496 | 0.0397 | 0.038 | 0.0198 | 0.0192 | 0.07 | 0.0738 |
Nucleus | 0.7806 | 0.9513 | 0.8625 | 0.8866 | 0.5978 | 0.8319 | 0.8427 | 0.9503 | 0.9481 | 0.738 | 0.7863 | 0.9243 | 0.808 | 0.9493 | 0.9237 | 0.7915 | 0.6107 | 0.8603 |
Peroxisomes | 0.0192 | 0 | 0.0074 | 0.009 | 0.0067 | 0.0014 | 0.0221 | 0.0011 | 0.0023 | 0.0066 | 0.005 | 0 | 0.0027 | 0 | 0.0058 | 0.0022 | 0.0211 | 0.0002 |
Punctate Nuclear | 0.0119 | 0.0056 | 0.0207 | 0.0025 | 0.0521 | 0.0252 | 0.0126 | 0.0074 | 0.0017 | 0.0491 | 0.0059 | 0.0197 | 0.0209 | 0.0095 | 0.0069 | 0.0097 | 0.0141 | 0.0179 |
Vacuole | 0.0064 | 0.0001 | 0.0032 | 0.0006 | 0.0368 | 0.0009 | 0.0015 | 0.0002 | 0.001 | 0.0034 | 0.0038 | 0.0009 | 0.0019 | 0.0001 | 0.0002 | 0.0008 | 0.0202 | 0.0004 |
Vacuole Periphery | 0.0021 | 0.0001 | 0.0015 | 0.0006 | 0.0123 | 0.0006 | 0.0012 | 0.0002 | 0.0005 | 0.0017 | 0.0036 | 0.0003 | 0.0016 | 0 | 0.0001 | 0.0011 | 0.0107 | 0.0008 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 17.3116 | 12.6849 | 10.5423 | 17.5672 | 14.7182 | 16.4511 | 14.8778 | 17.8323 | 15.1825 | 19.1619 |
Translational Efficiency | 1.4884 | 1.4191 | 1.3711 | 0.9113 | 1.2324 | 1.6429 | 0.9763 | 0.9969 | 1.1647 | 1.1419 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
76 | 1266 | 308 | 1753 | 867 | 1971 | 1186 | 66 | 943 | 3237 | 1494 | 1819 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 593.29 | 705.70 | 959.61 | 936.94 | 754.86 | 735.10 | 928.30 | 942.41 | 741.84 | 723.60 | 934.75 | 937.14 |
Standard Deviation | 70.70 | 110.96 | 137.29 | 129.73 | 83.04 | 106.91 | 124.55 | 163.46 | 93.15 | 109.45 | 127.91 | 131.11 |
Intensity Change Log 2 | — | 0.250318 | 0.693711 | 0.659219 | — | -0.038269 | 0.298382 | 0.320146 | — | 0.095889 | 0.485809 | 0.479253 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000072 | 0.001013 | 0.002102 | 0.001285 | 0.000340 | 0.000343 | 0.000991 | 0.001243 | 0.000319 | 0.000605 | 0.001220 | 0.001283 |
Bud Neck | 0.000963 | 0.009319 | 0.015698 | 0.024571 | 0.002654 | 0.011830 | 0.014679 | 0.005455 | 0.002517 | 0.010848 | 0.014889 | 0.023878 |
Bud Site | 0.000185 | 0.001957 | 0.002591 | 0.008963 | 0.000706 | 0.001488 | 0.001899 | 0.001989 | 0.000664 | 0.001672 | 0.002042 | 0.008710 |
Cell Periphery | 0.000172 | 0.000143 | 0.000109 | 0.000494 | 0.000054 | 0.000072 | 0.000101 | 0.000114 | 0.000064 | 0.000100 | 0.000103 | 0.000480 |
Cytoplasm | 0.099777 | 0.025429 | 0.002428 | 0.021217 | 0.012132 | 0.014831 | 0.006866 | 0.037290 | 0.019196 | 0.018976 | 0.005951 | 0.021800 |
Cytoplasmic Foci | 0.023405 | 0.004245 | 0.000090 | 0.001239 | 0.004230 | 0.002196 | 0.000153 | 0.000061 | 0.005775 | 0.002997 | 0.000140 | 0.001196 |
Eisosomes | 0.000064 | 0.000040 | 0.000053 | 0.000033 | 0.000035 | 0.000024 | 0.000034 | 0.000040 | 0.000037 | 0.000030 | 0.000037 | 0.000033 |
Endoplasmic Reticulum | 0.000590 | 0.003955 | 0.008891 | 0.005410 | 0.002223 | 0.002520 | 0.005586 | 0.005921 | 0.002091 | 0.003081 | 0.006267 | 0.005429 |
Endosome | 0.000707 | 0.001522 | 0.000394 | 0.002150 | 0.000898 | 0.001152 | 0.000480 | 0.000301 | 0.000882 | 0.001297 | 0.000462 | 0.002083 |
Golgi | 0.000227 | 0.001044 | 0.000194 | 0.001051 | 0.000544 | 0.000673 | 0.000140 | 0.000149 | 0.000518 | 0.000818 | 0.000151 | 0.001018 |
Lipid Particles | 0.005336 | 0.002121 | 0.000322 | 0.000237 | 0.001530 | 0.001125 | 0.000218 | 0.000233 | 0.001837 | 0.001514 | 0.000240 | 0.000237 |
Mitochondria | 0.002365 | 0.004308 | 0.001803 | 0.003444 | 0.001717 | 0.005839 | 0.001495 | 0.000969 | 0.001769 | 0.005240 | 0.001559 | 0.003354 |
Mitotic Spindle | 0.000087 | 0.009054 | 0.002536 | 0.029371 | 0.002525 | 0.012659 | 0.002779 | 0.004630 | 0.002329 | 0.011249 | 0.002729 | 0.028474 |
None | 0.058796 | 0.021305 | 0.003823 | 0.011904 | 0.007264 | 0.007376 | 0.005300 | 0.018499 | 0.011417 | 0.012824 | 0.004995 | 0.012144 |
Nuclear Periphery | 0.001708 | 0.001304 | 0.000564 | 0.002022 | 0.000912 | 0.003045 | 0.000786 | 0.000657 | 0.000976 | 0.002364 | 0.000740 | 0.001973 |
Nuclear Periphery Foci | 0.001037 | 0.002518 | 0.000394 | 0.000595 | 0.001186 | 0.000979 | 0.000450 | 0.000372 | 0.001174 | 0.001581 | 0.000438 | 0.000587 |
Nucleolus | 0.064526 | 0.067262 | 0.025850 | 0.012240 | 0.047134 | 0.058331 | 0.018028 | 0.019430 | 0.048536 | 0.061824 | 0.019640 | 0.012501 |
Nucleus | 0.736824* | 0.839713* | 0.927555* | 0.868432* | 0.912181* | 0.872019* | 0.936945* | 0.899548* | 0.898048* | 0.859384* | 0.935009* | 0.869561* |
Peroxisomes | 0.001775 | 0.001528 | 0.000715 | 0.000234 | 0.000823 | 0.000830 | 0.000290 | 0.000411 | 0.000900 | 0.001103 | 0.000378 | 0.000241 |
Vacuole | 0.001224 | 0.001442 | 0.003630 | 0.004604 | 0.000708 | 0.001869 | 0.002555 | 0.002525 | 0.000750 | 0.001702 | 0.002777 | 0.004528 |
Vacuole Periphery | 0.000159 | 0.000778 | 0.000258 | 0.000504 | 0.000205 | 0.000800 | 0.000226 | 0.000160 | 0.000201 | 0.000792 | 0.000233 | 0.000491 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -9.93 | -14.91 | -15.77 | -3.01 | 5.83 | -0.07 | -13.80 | -5.85 | -5.86 | -3.60 | -5.01 | -17.72 | -12.71 | -9.51 | 0.68 |
Bud Neck | -12.29 | -12.93 | -24.68 | -15.41 | -6.15 | -12.53 | -17.94 | -5.19 | -2.04 | 0.63 | -15.41 | -21.05 | -23.53 | -15.89 | -8.49 |
Bud Site | -9.12 | -10.75 | -8.81 | -5.92 | -3.65 | -2.41 | -5.85 | -4.86 | -4.29 | -3.54 | -3.91 | -7.49 | -8.07 | -6.80 | -5.42 |
Cell Periphery | 0.88 | 1.63 | -0.69 | -1.11 | -1.44 | -1.00 | -3.78 | -4.71 | -4.21 | -3.24 | -3.07 | -3.48 | -2.15 | -1.74 | -1.72 |
Cytoplasm | 3.44 | 4.59 | 3.91 | 3.79 | -10.30 | -1.10 | 3.60 | -1.25 | -1.05 | -1.89 | 0.11 | 5.72 | 1.13 | 1.70 | -8.43 |
Cytoplasmic Foci | 2.89 | 3.38 | 3.33 | 4.40 | -1.63 | 1.94 | 4.30 | 4.23 | 5.07 | -1.27 | 2.74 | 5.38 | 4.99 | 5.59 | -1.63 |
Eisosomes | 2.41 | 0.62 | 2.48 | 0.17 | 4.78 | 4.89 | -1.55 | -3.89 | -5.05 | -3.55 | 3.41 | -2.13 | -0.79 | -5.71 | 1.71 |
Endoplasmic Reticulum | -11.46 | -11.47 | -23.45 | -8.08 | 3.33 | -0.78 | -14.02 | -5.39 | -5.28 | -2.49 | -3.90 | -16.42 | -16.02 | -14.33 | 0.76 |
Endosome | -1.33 | 0.82 | -0.80 | 0.61 | -1.78 | -0.41 | 1.27 | 0.09 | 0.68 | -2.13 | -0.84 | 1.30 | -0.51 | 0.27 | -2.06 |
Golgi | -2.25 | 0.17 | -1.04 | 1.47 | -1.16 | -0.36 | 1.67 | 0.71 | 1.43 | -3.57 | -1.03 | 1.62 | 0.20 | 1.34 | -1.56 |
Lipid Particles | 3.29 | 4.92 | 5.02 | 2.96 | 1.66 | 1.65 | 5.90 | 4.00 | 1.94 | -2.29 | 1.79 | 7.14 | 7.04 | 4.32 | -0.56 |
Mitochondria | -2.10 | 0.95 | -1.81 | 1.02 | -6.58 | -4.55 | -0.23 | -0.68 | 4.79 | -0.97 | -4.90 | -0.21 | -3.55 | 2.95 | -9.24 |
Mitotic Spindle | -6.04 | -2.44 | -11.46 | -6.73 | -8.66 | -4.81 | -1.86 | -2.54 | -2.02 | -2.38 | -5.59 | -2.00 | -10.12 | -6.99 | -9.28 |
None | 3.10 | 4.66 | 4.18 | 7.48 | -7.29 | 0.03 | 3.78 | -1.10 | -1.11 | -1.70 | -0.75 | 5.87 | 2.20 | 4.39 | -6.49 |
Nuclear Periphery | 0.45 | 0.88 | -1.58 | -8.11 | -10.01 | -3.85 | -1.44 | -2.32 | 0.62 | -1.98 | -3.80 | -0.75 | -8.88 | -2.68 | -10.14 |
Nuclear Periphery Foci | -2.87 | 1.64 | 1.20 | 4.46 | -0.99 | 1.97 | 3.40 | 2.13 | 0.59 | -0.82 | -0.61 | 3.66 | 3.05 | 4.28 | -0.87 |
Nucleolus | 0.14 | 2.92 | 4.37 | 16.09 | 3.72 | -2.11 | 9.46 | 7.01 | 9.46 | 0.69 | -2.83 | 9.30 | 11.59 | 21.86 | 3.59 |
Nucleus | -3.50 | -5.94 | -4.24 | -2.78 | 6.57 | 5.19 | -3.16 | 3.03 | 1.80 | 3.73 | 5.25 | -4.77 | 4.83 | 0.06 | 12.06 |
Peroxisomes | 0.98 | 1.80 | 2.38 | 7.03 | 7.32 | -0.31 | 5.24 | 1.38 | 1.45 | -2.71 | -0.89 | 4.81 | 5.94 | 7.63 | 4.72 |
Vacuole | -0.15 | -3.63 | -6.40 | -8.81 | -0.97 | -3.14 | -5.39 | -2.99 | -2.84 | -2.42 | -3.18 | -6.73 | -10.26 | -9.02 | -3.79 |
Vacuole Periphery | -2.31 | -3.85 | -3.07 | 1.68 | -0.28 | -3.32 | 0.12 | -1.29 | 2.82 | -1.68 | -3.78 | -0.33 | -1.80 | 3.09 | -1.94 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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