Standard name
Human Ortholog
Description Phosphatidylserine decarboxylase of the mitochondrial inner membrane; converts phosphatidylserine to phosphatidylethanolamine; regulates mitochondrial fusion and morphology by affecting lipid mixing in the mitochondrial membrane and by influencing the ratio of long to short forms of Mgm1p; partly exposed to the mitochondrial intermembrane space; autocatalytically processed
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.07 – 0 0 0.09 0 0 0.07
Bud 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 – 0.07 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – – –
Cell Periphery 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 – 0.14 – 0 0 0 0 0 0
Cytoplasm 0.38 0.46 0.32 0.33 0.18 0.09 0.09 0.11 0.11 0.09 0.14 0.1 0.73 0.82 0.91 – 0.37 – 0.07 0.13 0 0.07 0.08 0.07
Endoplasmic Reticulum 0.16 0.16 0.11 0.07 0 0 0 0 0 0 0 0 0.09 0.08 0 – 0.12 – 0.19 0.19 0.16 0.1 0.05 0
Endosome 0.16 0.09 0.16 0.11 0.27 0 0 0 0 0 0 0 0.06 0 0 – 0.09 – 0.09 0.07 0.07 0.05 0.05 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Mitochondria 0.18 0.27 0.3 0.48 0.52 0.83 0.86 0.84 0.87 0.94 0.89 0.93 0 0 0 – 0.14 – 0.43 0.37 0.53 0.56 0.59 0.55
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0 0 0 – 0.12 – 0 0 0 0 0 0
Vac/Vac Membrane 0.19 0.09 0.13 0.1 0.15 0.11 0.12 0.05 0.07 0 0 0 0.09 0 0.06 – 0.09 – 0.07 0.1 0 0.09 0.07 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 11 15 3 2 0 0 0 0 0 0 0 0 7 6 1 – 3 – 18 22 33 10 16 28
Bud 2 11 4 7 5 11 12 17 14 21 25 24 0 1 0 – 3 – 5 5 2 3 6 9
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 2 0 1 – 1 – 9 11 4 5 13 16
Bud Site 0 0 0 0 1 2 6 5 3 8 4 5 0 0 0 – 1 – – – – – – –
Cell Periphery 4 6 10 8 2 1 1 3 3 2 9 5 4 6 3 – 6 – 4 5 3 1 4 6
Cytoplasm 108 176 79 53 47 34 43 65 40 42 63 50 137 146 183 – 16 – 35 67 11 20 29 29
Endoplasmic Reticulum 45 60 27 11 10 11 5 12 4 3 2 7 16 15 0 – 5 – 91 97 62 28 18 11
Endosome 45 33 40 18 72 7 9 23 5 2 1 0 11 5 4 – 4 – 41 38 27 14 18 23
Golgi 3 0 1 0 0 0 0 0 1 0 1 0 0 0 0 – 1 – 15 7 11 2 2 3
Mitochondria 51 103 75 78 137 314 391 495 315 450 385 456 6 0 1 – 6 – 208 193 205 157 227 229
Nucleus 0 1 0 0 0 6 9 15 8 1 6 3 1 1 1 – 0 – 0 1 0 0 0 1
Nuclear Periphery 2 0 0 0 0 1 2 7 1 4 1 7 0 0 0 – 1 – 2 1 2 0 4 1
Nucleolus 0 0 0 1 1 0 3 6 2 5 2 3 0 0 0 – 0 – 0 1 0 0 1 1
Peroxisomes 0 0 7 5 13 0 4 8 2 1 5 2 1 0 2 – 0 – 2 2 4 4 2 6
SpindlePole 2 1 10 3 8 6 8 31 7 12 15 25 1 1 3 – 5 – 6 6 4 2 8 8
Vac/Vac Membrane 55 33 31 16 41 43 55 30 27 19 12 6 16 8 13 – 4 – 35 50 8 26 26 32
Unique Cell Count 288 382 247 161 265 378 454 590 363 481 435 490 187 179 202 43 485 525 389 281 387 420
Labelled Cell Count 328 439 287 202 337 436 548 718 432 570 531 593 202 189 212 56 485 525 389 281 387 420


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 5.7 5.1 6.0 5.1 4.1 3.7 3.8 3.6 3.2 3.3 3.1 6.3 5.8 6.0 – 6.1 – 6.4 6.3 6.0
Std Deviation (1e-4) 0.8 0.9 1.3 6.6 1.4 1.2 1.1 1.2 0.9 0.9 0.9 0.9 1.0 1.1 1.3 – 1.6 – 1.3 1.6 1.4
Intensity Change (Log2) – – – 0.22 -0.0 -0.34 -0.48 -0.44 -0.52 -0.69 -0.64 -0.73 0.29 0.18 0.22 – 0.25 – 0.31 0.29 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Cell Periphery 0.4 -2.5 0 0 0 0 0 0 0 -1.1 -0.4 -1.6 – 0 –
Cytoplasm 0.2 -3.7 -7.3 -7.5 -7.3 -6.4 -8.0 -5.4 -7.3 8.5 10.1 12.5 – 0.7 –
Endoplasmic Reticulum -1.4 -3.1 0 0 0 0 0 0 0 -0.8 -0.9 -4.8 – 0 –
Endosome -1.4 3.0 0 0 0 0 0 0 0 -3.3 -4.4 -5.0 – -1.2 –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Mitochondria 3.7 4.9 0 0 0 0 0 0 0 -7.2 -8.1 -8.4 – -2.2 –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Peroxisomes 0 1.2 0 0 0 0 0 0 0 0 0 0 – 0 –
SpindlePole -1.2 -0.6 0 0 0 0 0 0 0 0 0 -1.6 – 0 –
Vacuole -0.8 1.0 -0.4 -0.2 -3.8 -2.1 -4.3 -5.1 -6.6 -1.3 -2.9 -2.2 – -0.6 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.9394 3.276 3.1754 2.5945 2.8552 3.0365 5.2063 6.1603 5.6254 5.2469 5.7572 5.9506 5.1371 5.9428 5.7749 5.6467 5.0116 5.4972
Actin 0.0268 0.0133 0.0248 0.0033 0.0084 0.0095 0.0145 0.0106 0.0185 0.0036 0.0168 0.0047 0.023 0.0101 0.012 0.0095 0.0057 0.0095
Bud 0.0024 0.0065 0.0038 0.0011 0.0026 0.0015 0.0046 0.0033 0.004 0.0029 0.0014 0.0013 0.0021 0.007 0.003 0.0023 0.0033 0.0017
Bud Neck 0.0063 0.0029 0.006 0.0103 0.001 0.0022 0.0021 0.0011 0.0071 0.0025 0.0008 0.0097 0.0014 0.0043 0.0035 0.0431 0.0011 0.0028
Bud Periphery 0.0069 0.0119 0.0081 0.0037 0.0089 0.0038 0.009 0.0053 0.0083 0.009 0.0019 0.0028 0.0044 0.0108 0.0056 0.0045 0.0164 0.0058
Bud Site 0.0077 0.0463 0.012 0.0016 0.0026 0.0015 0.0141 0.0252 0.0041 0.0049 0.0016 0.0028 0.0067 0.0564 0.0098 0.003 0.0026 0.0014
Cell Periphery 0.0028 0.0055 0.0059 0.002 0.0047 0.0015 0.0017 0.003 0.0022 0.0088 0.0003 0.0011 0.0021 0.0033 0.0045 0.0034 0.0019 0.0033
Cytoplasm 0.0399 0.0629 0.0545 0.0235 0.0319 0.0289 0.0324 0.0637 0.0408 0.0665 0.0258 0.0443 0.0359 0.0623 0.0256 0.0369 0.0142 0.0296
Cytoplasmic Foci 0.0576 0.0687 0.0462 0.0466 0.0589 0.0377 0.06 0.0893 0.0604 0.0286 0.0938 0.051 0.0612 0.065 0.0509 0.056 0.062 0.0458
Eisosomes 0.0005 0.0009 0.001 0.0012 0.0004 0.0004 0.0005 0.001 0.0006 0.0009 0.0002 0.0002 0.0012 0.0005 0.0011 0.0027 0.0003 0.0004
Endoplasmic Reticulum 0.0197 0.0226 0.0297 0.0066 0.0245 0.0207 0.0221 0.0256 0.021 0.0048 0.0475 0.016 0.014 0.0238 0.01 0.0054 0.0045 0.0184
Endosome 0.2148 0.1594 0.1736 0.1408 0.2104 0.1309 0.2076 0.1962 0.1555 0.0696 0.1039 0.1555 0.1725 0.1622 0.1309 0.0697 0.1539 0.1546
Golgi 0.1028 0.0985 0.1019 0.0955 0.0942 0.0899 0.1088 0.1105 0.1216 0.0428 0.1504 0.1094 0.1381 0.1301 0.1251 0.0791 0.1412 0.1361
Lipid Particles 0.0298 0.007 0.0119 0.0077 0.0126 0.01 0.0205 0.0131 0.0102 0.0018 0.0029 0.0056 0.0149 0.0033 0.0062 0.0011 0.0031 0.0064
Mitochondria 0.4038 0.4393 0.4819 0.593 0.4457 0.6068 0.4534 0.3975 0.498 0.7147 0.464 0.5562 0.4798 0.4194 0.581 0.6519 0.5666 0.5615
None 0.009 0.0146 0.0055 0.0014 0.0045 0.0111 0.0068 0.0201 0.0085 0.012 0.0284 0.0084 0.007 0.0084 0.0032 0.0014 0.0009 0.0012
Nuclear Periphery 0.0091 0.0021 0.002 0.0005 0.0043 0.0051 0.0037 0.0035 0.0028 0.0009 0.0027 0.0021 0.0015 0.002 0.0013 0.0004 0.0012 0.0012
Nucleolus 0.0007 0.001 0.0003 0.0003 0.0004 0.0022 0.0008 0.0004 0.0003 0.0003 0.0001 0.0003 0.0003 0.0028 0.0002 0.0003 0.0001 0.0002
Nucleus 0.0027 0.0018 0.001 0.0004 0.0016 0.0052 0.0013 0.0016 0.0008 0.0006 0.0003 0.0008 0.0008 0.0021 0.0006 0.0004 0.0005 0.0005
Peroxisomes 0.019 0.0102 0.0084 0.0126 0.0568 0.0152 0.0137 0.0075 0.0134 0.013 0.0481 0.0103 0.0161 0.0086 0.0145 0.0222 0.0084 0.0083
Punctate Nuclear 0.001 0.001 0.0004 0.0003 0.0019 0.0023 0.0025 0.0004 0.0003 0.0001 0.0003 0.0002 0.0006 0.0004 0.0003 0.0001 0.0002 0.0002
Vacuole 0.0264 0.0167 0.0124 0.015 0.0161 0.0071 0.0129 0.0148 0.0148 0.0062 0.0028 0.0095 0.0105 0.0127 0.0063 0.0035 0.0065 0.0059
Vacuole Periphery 0.0103 0.0067 0.0088 0.0325 0.0076 0.0066 0.0071 0.0062 0.0067 0.0056 0.0058 0.0078 0.0061 0.0044 0.0044 0.0029 0.0055 0.0054

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 30.0995 28.2197 24.8361 36.0447 42.3568 34.4852 28.6988 33.5891 37.9887 41.5672
Translational Efficiency 1.0921 0.8434 0.776 0.7476 0.6428 1.0387 0.8034 0.6877 0.6934 0.6934

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Phosphatidylserine decarboxylase of the mitochondrial inner membrane; converts phosphatidylserine to phosphatidylethanolamine; regulates mitochondrial fusion and morphology by affecting lipid mixing in the mitochondrial membrane and by influencing the ratio of long to short forms of Mgm1p; partly exposed to the mitochondrial intermembrane space; autocatalytically processed
Localization
Cell Percentages mitochondrion (42%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Psd1

Psd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Psd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available