Standard name | |
---|---|
Human Ortholog | |
Description | Putative oxidoreductase; required for cell viability |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 4 | 38 | 35 | 36 | 43 | 49 | 29 | 22 | 20 | 21 | 5 | 5 | 17 | 8 | 18 | 13 | 14 | 5 | 4 | 18 | 5 | 12 | 8 |
Bud | 1 | 1 | 10 | 9 | 17 | 16 | 37 | 21 | 28 | 25 | 20 | 28 | 0 | 0 | 0 | 2 | 2 | 2 | 0 | 0 | 0 | 1 | 2 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 3 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 4 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 3 | 22 | 32 | 20 | 22 | 19 | 21 | 9 | 14 | 14 | 21 | 9 | 40 | 34 | 17 | 23 | 9 | 0 | 0 | 6 | 3 | 5 | 6 |
Cytoplasm | 12 | 18 | 50 | 40 | 65 | 49 | 75 | 64 | 58 | 82 | 56 | 68 | 46 | 89 | 102 | 84 | 65 | 37 | 3 | 6 | 3 | 20 | 27 | 34 |
Endoplasmic Reticulum | 28 | 16 | 80 | 53 | 37 | 100 | 104 | 42 | 67 | 29 | 35 | 16 | 78 | 111 | 151 | 6 | 3 | 2 | 65 | 51 | 155 | 79 | 135 | 135 |
Endosome | 0 | 0 | 8 | 5 | 26 | 18 | 21 | 52 | 7 | 21 | 8 | 18 | 1 | 0 | 0 | 1 | 0 | 5 | 1 | 0 | 0 | 2 | 2 | 0 |
Golgi | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 5 | 1 | 1 | 3 | 1 | 1 | 1 |
Mitochondria | 6 | 2 | 6 | 25 | 29 | 59 | 104 | 69 | 75 | 115 | 122 | 109 | 4 | 1 | 1 | 5 | 1 | 2 | 1 | 1 | 6 | 11 | 13 | 8 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 3 | 0 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 6 | 11 | 12 | 8 | 10 | 8 | 3 | 4 | 5 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 2 | 1 | 4 | 8 | 3 | 2 | 1 | 2 | 2 | 2 | 2 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 0 | 6 | 1 | 2 | 4 | 7 | 7 | 10 | 13 | 10 | 14 | 0 | 27 | 13 | 18 | 5 | 14 | 5 | 3 | 2 | 7 | 16 | 16 |
Unique Cell Count | 45 | 39 | 168 | 147 | 177 | 241 | 316 | 251 | 222 | 255 | 230 | 229 | 137 | 237 | 275 | 140 | 100 | 79 | 86 | 75 | 206 | 140 | 228 | 223 |
Labelled Cell Count | 53 | 44 | 226 | 213 | 248 | 321 | 431 | 327 | 285 | 329 | 298 | 287 | 149 | 287 | 314 | 155 | 114 | 94 | 86 | 75 | 206 | 140 | 228 | 223 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.2 | 7.9 | 7.4 | 7.2 | 7.5 | 6.8 | 6.4 | 6.4 | 5.9 | 5.7 | 5.2 | 5.3 | 8.3 | 8.7 | 8.4 | 13.1 | 13.1 | 12.9 | 8.7 | 9.2 | 9.6 |
Std Deviation (1e-4) | 0.8 | 1.2 | 1.1 | 1.8 | 2.0 | 1.2 | 1.3 | 1.6 | 1.3 | 1.2 | 1.0 | 1.0 | 1.4 | 1.1 | 1.3 | 2.7 | 3.0 | 2.5 | 1.3 | 1.6 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.03 | -0.13 | -0.21 | -0.21 | -0.31 | -0.37 | -0.49 | -0.47 | 0.18 | 0.24 | 0.18 | 0.83 | 0.83 | 0.81 | 0.24 | 0.32 | 0.38 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.2 | -0.5 | -1.2 | -1.9 | -3.0 | -3.4 | -4.3 | -3.7 | -6.5 | -4.7 | -4.5 | -6.6 | -2.2 | -1.9 | -0.9 |
Bud | 0.1 | 1.3 | 0.3 | 2.0 | 0.9 | 2.2 | 1.4 | 1.0 | 2.1 | 0 | 0 | 0 | -2.0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 2.0 | -0.5 | -1.3 | -2.7 | -1.6 | -3.3 | -2.7 | -2.4 | -1.2 | -1.9 | 1.0 | -0.2 | -0.3 | 2.1 | -0.4 |
Cytoplasm | -0.5 | 1.4 | -2.2 | -1.4 | -1.0 | -0.8 | 0.5 | -1.2 | 0 | 0.7 | 1.6 | 1.6 | 5.3 | 5.6 | 2.6 |
Endoplasmic Reticulum | -2.1 | -5.2 | -1.2 | -3.2 | -6.8 | -3.5 | -8.3 | -7.0 | -9.3 | 1.6 | -0.2 | 1.5 | -8.4 | -7.6 | -7.0 |
Endosome | -0.6 | 3.1 | 1.1 | 0.8 | 4.6 | -0.8 | 1.4 | -0.6 | 1.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.0 | 3.9 | 5.7 | 7.3 | 6.3 | 7.3 | 9.2 | 10.4 | 9.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1.5 | 1.3 | -0.1 | -0.2 | -0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | -0.5 | 0.5 | 0.7 | 0.4 | 1.1 | 0 | 2.8 | 0.6 | 3.0 | 0 | 3.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.7529 | 4.0374 | 3.0047 | 2.8927 | 2.5451 | 3.1518 | 3.399 | 4.4304 | 3.8409 | 3.9088 | 3.6707 | 3.9343 | 3.3693 | 4.4511 | 3.8129 | 3.4118 | 3.1691 | 3.943 |
Actin | 0.0243 | 0.0081 | 0.0311 | 0.1054 | 0.0452 | 0.0086 | 0.0438 | 0.0138 | 0.0438 | 0.0304 | 0.0047 | 0.0167 | 0.0135 | 0.0033 | 0.0199 | 0.0118 | 0.0112 | 0.0064 |
Bud | 0.0006 | 0.0012 | 0.0009 | 0.0005 | 0.0013 | 0.0005 | 0.0012 | 0.0004 | 0.0007 | 0.0007 | 0.0001 | 0.0002 | 0.002 | 0.0003 | 0.0012 | 0.0001 | 0.0026 | 0.0003 |
Bud Neck | 0.0017 | 0.0026 | 0.0054 | 0.0019 | 0.0173 | 0.0085 | 0.0109 | 0.0028 | 0.0042 | 0.0216 | 0.0016 | 0.0139 | 0.0017 | 0.0036 | 0.0085 | 0.0022 | 0.0052 | 0.0088 |
Bud Periphery | 0.0017 | 0.0031 | 0.0034 | 0.0022 | 0.0042 | 0.0018 | 0.0023 | 0.0007 | 0.0034 | 0.0062 | 0.0003 | 0.0006 | 0.0014 | 0.0006 | 0.0013 | 0.0004 | 0.0016 | 0.0005 |
Bud Site | 0.0056 | 0.0686 | 0.0136 | 0.0023 | 0.0045 | 0.0007 | 0.0101 | 0.0284 | 0.0044 | 0.0025 | 0.0003 | 0.0003 | 0.003 | 0.0271 | 0.0257 | 0.0012 | 0.0016 | 0.0004 |
Cell Periphery | 0.0161 | 0.0251 | 0.0373 | 0.0082 | 0.0119 | 0.0093 | 0.0051 | 0.0246 | 0.0213 | 0.009 | 0.009 | 0.0027 | 0.0074 | 0.0209 | 0.0202 | 0.0058 | 0.0048 | 0.0043 |
Cytoplasm | 0.2485 | 0.2962 | 0.3033 | 0.1458 | 0.2154 | 0.2555 | 0.1889 | 0.3569 | 0.3065 | 0.2825 | 0.2416 | 0.3542 | 0.1891 | 0.2306 | 0.2365 | 0.1665 | 0.2149 | 0.2514 |
Cytoplasmic Foci | 0.026 | 0.0157 | 0.0219 | 0.0244 | 0.019 | 0.0198 | 0.0387 | 0.0133 | 0.0161 | 0.0208 | 0.012 | 0.0145 | 0.0208 | 0.0162 | 0.0227 | 0.0178 | 0.0248 | 0.0148 |
Eisosomes | 0.0015 | 0.0013 | 0.0018 | 0.0022 | 0.0007 | 0.0005 | 0.0008 | 0.0004 | 0.0005 | 0.0001 | 0.0003 | 0.0002 | 0.0006 | 0.0004 | 0.0007 | 0.0003 | 0.0002 | 0.0002 |
Endoplasmic Reticulum | 0.5305 | 0.4367 | 0.4109 | 0.6037 | 0.4074 | 0.5558 | 0.4744 | 0.4689 | 0.486 | 0.5061 | 0.5435 | 0.508 | 0.6219 | 0.5704 | 0.5562 | 0.7043 | 0.5625 | 0.6318 |
Endosome | 0.0297 | 0.019 | 0.0397 | 0.0295 | 0.0676 | 0.0495 | 0.0577 | 0.0225 | 0.025 | 0.036 | 0.0304 | 0.027 | 0.033 | 0.0345 | 0.0223 | 0.0166 | 0.0331 | 0.0227 |
Golgi | 0.0174 | 0.0175 | 0.0224 | 0.0242 | 0.0278 | 0.0165 | 0.0223 | 0.011 | 0.0223 | 0.0167 | 0.0186 | 0.015 | 0.0118 | 0.0098 | 0.0136 | 0.0306 | 0.0156 | 0.0087 |
Lipid Particles | 0.0437 | 0.0289 | 0.0353 | 0.0251 | 0.017 | 0.0233 | 0.0465 | 0.0339 | 0.0271 | 0.0128 | 0.067 | 0.0143 | 0.0358 | 0.0551 | 0.0365 | 0.0255 | 0.0566 | 0.0219 |
Mitochondria | 0.0076 | 0.0085 | 0.0137 | 0.0029 | 0.0538 | 0.0061 | 0.0288 | 0.0016 | 0.0062 | 0.0018 | 0.0514 | 0.0061 | 0.003 | 0.0011 | 0.0079 | 0.0012 | 0.0175 | 0.0013 |
None | 0.0248 | 0.0508 | 0.03 | 0.0041 | 0.0144 | 0.0266 | 0.0057 | 0.004 | 0.0054 | 0.0031 | 0.0049 | 0.006 | 0.0282 | 0.0061 | 0.0059 | 0.002 | 0.0077 | 0.0137 |
Nuclear Periphery | 0.0062 | 0.0031 | 0.0065 | 0.0054 | 0.0417 | 0.0048 | 0.0195 | 0.0047 | 0.0075 | 0.0089 | 0.0047 | 0.0087 | 0.0102 | 0.0054 | 0.0059 | 0.0053 | 0.0055 | 0.0042 |
Nucleolus | 0.0002 | 0.0007 | 0.0007 | 0.0001 | 0.0014 | 0.0001 | 0.0011 | 0.0002 | 0.0002 | 0.0015 | 0.0001 | 0.0002 | 0.0008 | 0.0003 | 0.0008 | 0 | 0.0025 | 0.0001 |
Nucleus | 0.0012 | 0.0013 | 0.0024 | 0.0009 | 0.0068 | 0.0013 | 0.0042 | 0.0013 | 0.0049 | 0.0078 | 0.0012 | 0.0018 | 0.0038 | 0.0013 | 0.0027 | 0.0008 | 0.0082 | 0.0012 |
Peroxisomes | 0.0033 | 0.0004 | 0.0022 | 0.0007 | 0.0034 | 0.0007 | 0.0191 | 0.0003 | 0.0058 | 0.0054 | 0.0016 | 0.0008 | 0.001 | 0.0003 | 0.0033 | 0.0008 | 0.006 | 0.0007 |
Punctate Nuclear | 0.0022 | 0.0006 | 0.0043 | 0.0005 | 0.0237 | 0.0006 | 0.0041 | 0.0006 | 0.0004 | 0.0095 | 0.0013 | 0.0028 | 0.0042 | 0.0011 | 0.0024 | 0.0007 | 0.0126 | 0.0013 |
Vacuole | 0.0065 | 0.0098 | 0.0125 | 0.009 | 0.0122 | 0.0083 | 0.011 | 0.0092 | 0.0068 | 0.0157 | 0.0043 | 0.005 | 0.0058 | 0.0111 | 0.0052 | 0.0059 | 0.0047 | 0.0049 |
Vacuole Periphery | 0.0007 | 0.0006 | 0.0009 | 0.001 | 0.0031 | 0.0011 | 0.0038 | 0.0005 | 0.0015 | 0.0007 | 0.0011 | 0.0009 | 0.0009 | 0.0007 | 0.0006 | 0.0004 | 0.0006 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 37.4112 | 24.1759 | 21.5275 | 25.0258 | 35.6484 | 44.1478 | 23.8331 | 24.4723 | 24.831 | 33.5488 |
Translational Efficiency | 1.6825 | 1.3846 | 1.3854 | 1.462 | 1.3811 | 1.4149 | 1.5579 | 1.2782 | 1.3686 | 1.2667 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
135 | 144 | 2216 | 1515 | 1814 | 1965 | 134 | 1530 | 1949 | 2109 | 2350 | 3045 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 910.31 | 1039.27 | 1072.11 | 1196.56 | 847.33 | 942.48 | 1269.17 | 1196.96 | 851.69 | 949.09 | 1083.35 | 1196.76 |
Standard Deviation | 107.21 | 140.80 | 140.36 | 161.62 | 116.25 | 114.25 | 168.64 | 175.21 | 116.75 | 118.79 | 149.29 | 168.59 |
Intensity Change Log 2 | — | 0.191141 | 0.236023 | 0.394463 | — | 0.153538 | 0.582889 | 0.498379 | — | 0.173135 | 0.413658 | 0.445494 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.015061 | 0.015045 | 0.012962 | 0.119240* | 0.006487 | 0.004600 | 0.029093 | 0.051904 | 0.007081 | 0.005313 | 0.013882 | 0.085406 |
Bud Neck | 0.004875 | 0.008228 | 0.008291 | 0.024433 | 0.003248 | 0.006301 | 0.008458 | 0.021703 | 0.003361 | 0.006433 | 0.008301 | 0.023062 |
Bud Site | 0.009765 | 0.017506 | 0.019795 | 0.144156* | 0.009691 | 0.012058 | 0.040907 | 0.096584 | 0.009696 | 0.012430 | 0.020999 | 0.120253* |
Cell Periphery | 0.019481 | 0.020252 | 0.009508 | 0.059360 | 0.002379 | 0.010126 | 0.027892 | 0.059369 | 0.003564 | 0.010818 | 0.010556 | 0.059364 |
Cytoplasm | 0.135703* | 0.103780* | 0.167696* | 0.101209* | 0.172128* | 0.200017* | 0.040417 | 0.137820* | 0.169605* | 0.193446* | 0.160439* | 0.119604* |
Cytoplasmic Foci | 0.047385 | 0.051245 | 0.020077 | 0.028557 | 0.076300 | 0.043268 | 0.015549 | 0.024415 | 0.074297 | 0.043813 | 0.019819 | 0.026476 |
Eisosomes | 0.006771 | 0.006939 | 0.003955 | 0.009617 | 0.002732 | 0.005658 | 0.003940 | 0.010023 | 0.003012 | 0.005745 | 0.003954 | 0.009821 |
Endoplasmic Reticulum | 0.470372* | 0.548934* | 0.607484* | 0.304653* | 0.453622* | 0.508881* | 0.708903* | 0.385702* | 0.454782* | 0.511616* | 0.613267* | 0.345378* |
Endosome | 0.018351 | 0.025388 | 0.009685 | 0.020954 | 0.015377 | 0.018747 | 0.009569 | 0.018926 | 0.015583 | 0.019200 | 0.009679 | 0.019935 |
Golgi | 0.097134 | 0.056430 | 0.009876 | 0.018917 | 0.081554 | 0.049981 | 0.006436 | 0.017899 | 0.082634 | 0.050422 | 0.009680 | 0.018405 |
Lipid Particles | 0.113195* | 0.074078 | 0.052068 | 0.100388* | 0.115472* | 0.072494 | 0.032877 | 0.100153* | 0.115314* | 0.072602 | 0.050973 | 0.100269* |
Mitochondria | 0.003358 | 0.003727 | 0.001767 | 0.004301 | 0.013324 | 0.011242 | 0.000073 | 0.003688 | 0.012634 | 0.010729 | 0.001671 | 0.003993 |
Mitotic Spindle | 0.000105 | 0.000666 | 0.000204 | 0.003077 | 0.000513 | 0.001056 | 0.000180 | 0.003702 | 0.000485 | 0.001029 | 0.000203 | 0.003391 |
None | 0.000165 | 0.000773 | 0.003309 | 0.000252 | 0.005651 | 0.002091 | 0.000072 | 0.001677 | 0.005271 | 0.002001 | 0.003124 | 0.000968 |
Nuclear Periphery | 0.000725 | 0.000205 | 0.002251 | 0.000187 | 0.000937 | 0.000387 | 0.000326 | 0.000295 | 0.000923 | 0.000375 | 0.002141 | 0.000241 |
Nuclear Periphery Foci | 0.004048 | 0.003934 | 0.012365 | 0.004386 | 0.007563 | 0.002481 | 0.015413 | 0.007016 | 0.007320 | 0.002580 | 0.012539 | 0.005708 |
Nucleolus | 0.000039 | 0.000604 | 0.000126 | 0.000064 | 0.000215 | 0.000226 | 0.000017 | 0.000096 | 0.000203 | 0.000252 | 0.000120 | 0.000080 |
Nucleus | 0.003409 | 0.002294 | 0.028089 | 0.004078 | 0.011115 | 0.006782 | 0.004884 | 0.007622 | 0.010581 | 0.006475 | 0.026765 | 0.005859 |
Peroxisomes | 0.030246 | 0.028892 | 0.004213 | 0.016545 | 0.012922 | 0.022111 | 0.001210 | 0.012626 | 0.014122 | 0.022574 | 0.004042 | 0.014576 |
Vacuole | 0.017812 | 0.029793 | 0.025933 | 0.033344 | 0.006875 | 0.017717 | 0.053636 | 0.036955 | 0.007632 | 0.018541 | 0.027513 | 0.035158 |
Vacuole Periphery | 0.002000 | 0.001287 | 0.000344 | 0.002283 | 0.001896 | 0.003776 | 0.000150 | 0.001825 | 0.001904 | 0.003606 | 0.000333 | 0.002053 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.00 | 0.34 | -13.05 | -14.57 | -20.17* | 2.16 | -3.14 | -14.94 | -15.78 | -2.95 | 1.81 | -4.87 | -25.11 | -26.06* | -22.09* |
Bud Neck | -1.16 | -1.86 | -8.73 | -5.87 | -9.70 | -3.82 | -2.86 | -12.83 | -10.01 | -5.60 | -3.99 | -6.04 | -18.10 | -13.78 | -11.89 |
Bud Site | -1.29 | -2.48 | -21.65 | -18.42 | -23.75* | -1.45 | -3.53 | -19.88 | -18.99 | -5.37 | -1.74 | -6.08 | -32.17 | -30.53 | -27.01* |
Cell Periphery | -0.09 | 1.67 | -5.85 | -5.82 | -16.15 | -7.97 | -5.20 | -20.59 | -16.78 | -5.59 | -6.73 | -8.64 | -26.64 | -21.32 | -22.67 |
Cytoplasm | 1.67 | -2.20 | 2.27 | 0.13 | 11.54 | -4.57 | 15.67 | 5.14 | 9.49 | -11.48 | -4.07 | 1.48 | 9.47 | 13.99 | 7.99 |
Cytoplasmic Foci | -0.36 | 3.96 | 2.70 | 2.47 | -3.86 | 10.07 | 11.37 | 16.65 | 7.79 | -1.66 | 9.66 | 19.27 | 16.93 | 7.95 | -3.95 |
Eisosomes | -0.08 | 1.96 | -1.79 | -1.77 | -8.81 | -5.46 | -1.27 | -9.84 | -5.26 | -5.49 | -5.29 | -2.23 | -12.89 | -6.48 | -10.95 |
Endoplasmic Reticulum | -1.79 | -4.32 | 5.42 | 7.58 | 26.76 | -5.04 | -7.93 | 6.11 | 10.97 | 10.22 | -5.34 | -14.70 | 11.48 | 17.65 | 28.22 |
Endosome | -1.47 | 3.04 | -0.92 | 1.04 | -8.29 | -2.82 | 3.38 | -2.40 | -0.10 | -4.59 | -3.12 | 6.78 | -3.92 | -0.61 | -9.79 |
Golgi | 2.92 | 7.96 | 7.09 | 4.27 | -5.54 | 9.96 | 26.36 | 21.09 | 13.52 | -5.79 | 10.37 | 28.27 | 23.35 | 15.37 | -7.17 |
Lipid Particles | 2.41 | 4.57 | 0.94 | -2.50 | -9.72 | 9.48 | 10.32 | 2.78 | -5.37 | -8.01 | 9.78 | 15.08 | 3.28 | -6.92 | -12.77 |
Mitochondria | -0.21 | 2.50 | -0.96 | -0.27 | -3.45 | 1.51 | 13.78 | 9.14 | 7.05 | -7.94 | 1.48 | 12.11 | 8.77 | 6.69 | -5.21 |
Mitotic Spindle | -1.13 | -2.75 | -3.79 | -2.68 | -3.67 | -1.36 | 2.12 | -3.70 | -2.80 | -4.09 | -1.46 | 2.12 | -4.94 | -3.46 | -5.49 |
None | -1.12 | -5.34 | -0.83 | 0.97 | 5.24 | 3.94 | 6.66 | 4.06 | 0.69 | -3.03 | 3.88 | 2.28 | 5.25 | 2.51 | 3.55 |
Nuclear Periphery | 2.45 | -3.50 | 2.55 | 0.14 | 5.30 | 3.36 | 3.57 | 3.92 | 2.39 | 0.54 | 3.58 | -3.11 | 4.45 | 4.01 | 5.18 |
Nuclear Periphery Foci | 0.05 | -6.97 | -0.62 | -0.67 | 7.23 | 6.39 | -2.98 | 0.26 | -5.94 | 3.08 | 6.36 | -4.82 | 1.53 | -6.39 | 6.76 |
Nucleolus | -1.01 | -8.97 | -2.20 | 0.96 | 3.59 | -0.17 | 11.34 | 5.46 | 1.73 | -6.43 | -0.64 | 4.57 | 6.40 | 2.15 | 2.98 |
Nucleus | 1.38 | -14.63 | -1.48 | -3.03 | 14.54 | 5.11 | 3.71 | 3.28 | -2.06 | -1.93 | 5.18 | -9.94 | 5.74 | 0.59 | 13.31 |
Peroxisomes | 0.17 | 4.59 | 2.40 | 2.12 | -9.55 | -5.88 | 14.57 | 0.41 | 5.85 | -11.54 | -5.41 | 11.41 | -0.21 | 5.34 | -12.34 |
Vacuole | -1.63 | -1.99 | -3.68 | -0.80 | -3.36 | -8.00 | -4.87 | -15.10 | -9.08 | 1.74 | -7.99 | -12.92 | -18.53 | -9.87 | -4.26 |
Vacuole Periphery | 1.40 | 3.59 | -0.45 | -2.10 | -4.61 | -3.07 | 11.19 | 0.23 | 2.81 | -4.38 | -2.98 | 10.52 | -0.42 | 2.53 | -6.04 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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