ORF
Human Ortholog
Description Hydrophilin essential in desiccation-rehydration process; cell wall protein; contains a putative GPI-attachment site
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.17 0.39 0.51 0.55 0.53 0.69 0.75 0.79 0.81 0.84 0.85 0.83 0.69 0.91 0.95 0.19 0.19 0.22 0 0 0 0.07 0.06 0.06
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Endosome 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.06 0 0 0 0.09 0 0 0.05 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0.17 0.07 0.07 0.08 0.07 0.08 0 0 0 0 0 0 0 0 0 0.38 0.38 0.35 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.73 0.68 0.59 0.61 0.61 0.37 0.42 0.29 0.29 0.2 0.2 0.21 0.35 0.13 0.07 0.54 0.56 0.55 0.88 0.9 0.9 0.84 0.79 0.76
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1
Bud 0 1 0 0 0 6 2 4 3 3 3 5 0 0 0 1 1 0 0 4 2 1 7 11
Bud Neck 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 50 227 206 175 226 347 530 685 563 599 574 542 135 261 323 62 57 62 4 21 11 22 28 33
Endoplasmic Reticulum 2 1 0 0 1 1 0 1 1 1 0 0 3 0 0 20 9 13 1 2 1 1 3 4
Endosome 22 10 11 5 5 2 11 5 6 3 6 9 6 8 3 12 7 8 10 9 5 8 22 39
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 2 1 0 0 4 1
Mitochondria 24 34 2 4 5 44 8 17 33 23 28 23 1 0 0 9 5 14 7 10 5 5 9 12
Nucleus 48 43 30 27 31 42 16 26 24 21 14 18 2 2 0 120 113 97 4 3 3 1 3 5
Nuclear Periphery 3 2 0 0 0 0 1 3 2 6 3 4 0 0 0 3 0 3 1 1 1 1 5 5
Nucleolus 2 0 0 1 0 0 1 2 0 1 0 1 0 1 0 0 1 2 1 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 1 1 0 1 1 0 1 0 1 0 1 1 1 1 2 4 2 1 4 2 1 4 3
Vac/Vac Membrane 212 399 238 195 260 186 292 247 200 145 135 139 68 37 23 173 168 151 330 569 386 275 363 419
Unique Cell Count 289 586 402 319 425 505 702 866 696 716 679 651 197 287 341 318 298 277 374 635 430 327 459 549
Labelled Cell Count 363 718 491 407 529 629 862 992 832 804 764 743 216 311 350 403 367 353 374 635 430 327 459 549


Cell Periphery, Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 4.8 5.8 5.8 6.4 4.4 5.4 5.4 4.8 5.1 4.9 4.9 5.4 5.1 5.3 7.3 7.6 6.7 4.6 5.3 5.3
Std Deviation (1e-4) 1.4 0.9 1.3 1.5 1.6 1.2 1.4 1.4 1.1 1.4 1.1 1.0 1.2 1.6 1.7 2.2 2.0 2.0 1.5 2.0 1.7
Intensity Change (Log2) – – – -0.01 0.14 -0.41 -0.1 -0.11 -0.28 -0.19 -0.26 -0.26 -0.12 -0.19 -0.14 0.32 0.38 0.19 -0.33 -0.14 -0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – -1.3 -0.7 -1.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.2361 1.129 0.7219 0.0971 0.2632 0.6534 1.3086 2.9282 2.4186 2.2159 1.6405 2.1719 3.4629 5.8183 4.5166 4.2492 4.3315 4.4405
Actin 0.0003 0 0.0001 0 0 0 0.0001 0.0001 0 0.0005 0 0 0.0121 0 0.0001 0 0 0
Bud 0.001 0.001 0.0011 0.0003 0.0003 0.0004 0.0004 0.0007 0.0006 0.0006 0.0009 0.0006 0.0011 0.0001 0.0004 0.0001 0.0003 0.0004
Bud Neck 0.0013 0.0011 0.0013 0.0009 0.0014 0.0013 0.0018 0.0082 0.0011 0.002 0.0027 0.0038 0.0005 0.0007 0.0012 0.0003 0.0017 0.0016
Bud Periphery 0.0009 0.002 0.0013 0.0012 0.0007 0.0009 0.0015 0.0014 0.0009 0.0009 0.0036 0.0019 0.0061 0.0005 0.0019 0.0002 0.0009 0.0013
Bud Site 0.0019 0.002 0.0023 0.0006 0.0064 0.0006 0.0017 0.0059 0.0017 0.0012 0.0013 0.0015 0.0014 0.0006 0.0017 0.0002 0.001 0.0013
Cell Periphery 0.0011 0.0021 0.0013 0.0006 0.0013 0.0015 0.0017 0.0025 0.0013 0.001 0.0009 0.0023 0.0029 0.0057 0.003 0.001 0.0073 0.0037
Cytoplasm 0.0251 0.0164 0.0169 0.0162 0.0109 0.0173 0.0141 0.0155 0.0202 0.0091 0.0115 0.0154 0.0237 0.0019 0.003 0.0136 0.0013 0.0133
Cytoplasmic Foci 0.0422 0.0108 0.0364 0.0255 0.0147 0.0294 0.0158 0.0209 0.0224 0.0221 0.0156 0.0159 0.0211 0.0013 0.0091 0.0194 0.0025 0.0065
Eisosomes 0 0 0 0 0 0 0 0 0 0.0002 0 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0049 0.0047 0.0037 0.0011 0.0007 0.003 0.0039 0.0036 0.0031 0.0021 0.0025 0.0038 0.0094 0.0016 0.0018 0.0016 0.0012 0.003
Endosome 0.2888 0.1971 0.2814 0.3726 0.2823 0.2507 0.2792 0.1901 0.2845 0.2324 0.303 0.2328 0.1884 0.1042 0.2001 0.2606 0.171 0.1495
Golgi 0.0046 0.0032 0.0074 0.0044 0.002 0.0057 0.0053 0.0081 0.0096 0.0055 0.0048 0.0044 0.0067 0.001 0.0076 0.0042 0.0024 0.0036
Lipid Particles 0.0182 0.0059 0.0146 0.0065 0.0048 0.0088 0.0119 0.0105 0.0109 0.0052 0.0052 0.0111 0.0127 0.0019 0.0288 0.0246 0.0053 0.0233
Mitochondria 0.0148 0.0071 0.0258 0.0053 0.0006 0.0076 0.0121 0.0059 0.0168 0.0328 0.007 0.0073 0.0281 0.0005 0.1123 0.016 0.0007 0.0205
None 0.0146 0.0195 0.0267 0.0987 0.0013 0.0211 0.0206 0.0039 0.0221 0.0238 0.0052 0.014 0.0379 0.0001 0.002 0.012 0.0001 0.002
Nuclear Periphery 0.0083 0.0108 0.0097 0.0019 0.0014 0.0151 0.0066 0.0058 0.0047 0.0024 0.0046 0.0063 0.0165 0.0031 0.0023 0.002 0.0016 0.0142
Nucleolus 0.0123 0.0035 0.0056 0.0027 0.0064 0.0033 0.0063 0.0068 0.0074 0.0036 0.0087 0.0143 0.002 0.0018 0.0059 0.0011 0.0056 0.0083
Nucleus 0.0064 0.0057 0.0059 0.0027 0.0033 0.0079 0.008 0.0153 0.0089 0.0052 0.0225 0.0175 0.0049 0.0024 0.0028 0.0013 0.0031 0.018
Peroxisomes 0.0024 0.0008 0.0027 0.0011 0.0009 0.0012 0.0013 0.007 0.0014 0.0012 0.0008 0.0014 0.0027 0.0001 0.0023 0.0011 0.0004 0.0008
Punctate Nuclear 0.0024 0.0004 0.0027 0.0005 0.0004 0.0009 0.0021 0.0009 0.0029 0.0007 0.0048 0.0009 0.0019 0 0.0002 0.0004 0 0.0002
Vacuole 0.4393 0.5826 0.4456 0.3546 0.5826 0.4908 0.4796 0.5821 0.4738 0.5419 0.511 0.5469 0.4882 0.7458 0.482 0.4954 0.7036 0.5489
Vacuole Periphery 0.1092 0.1233 0.1074 0.1024 0.0776 0.1322 0.1264 0.1048 0.1058 0.1055 0.0834 0.0979 0.1315 0.1266 0.1315 0.1447 0.09 0.1797

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 346.2716 421.8162 484.3854 624.5262 388.4752 306.6304 627.5843 714.718 548.7602 435.8193
Translational Efficiency 0.7348 0.8954 1.2088 0.6215 0.7418 0.7991 0.7921 1.0068 1.073 0.801

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2172 1362 1957 1272 1388 2447 2477 1413 3560 3809 4434 2685

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 752.60 831.38 820.90 798.00 664.17 806.07 815.84 850.38 718.12 815.12 818.07 825.57
Standard Deviation 89.58 110.25 98.99 106.68 85.48 102.33 95.83 123.51 98.01 105.93 97.27 118.75
Intensity Change Log 2 — 0.143625 0.125323 0.084505 — 0.279353 0.296734 0.356555 — 0.208845 0.208220 0.218443

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000014 0.000078 0.000127 0.000331 0.000048 0.000063 0.000076 0.000682 0.000027 0.000068 0.000099 0.000516
Bud Neck 0.013435 0.014836 0.030330 0.031041 0.024478 0.018489 0.038897 0.022206 0.017740 0.017183 0.035116 0.026392
Bud Site 0.005756 0.017815 0.010203 0.023069 0.008654 0.015601 0.009987 0.030103 0.006886 0.016393 0.010082 0.026770
Cell Periphery 0.000342 0.000212 0.000054 0.000047 0.000189 0.000543 0.000094 0.000081 0.000282 0.000425 0.000077 0.000065
Cytoplasm 0.035502 0.112460* 0.412891* 0.174048* 0.141614* 0.039045 0.147140* 0.165050* 0.076874 0.065296 0.264432* 0.169313*
Cytoplasmic Foci 0.015060 0.038558 0.015780 0.067285 0.048790 0.014450 0.029771 0.055677 0.028211 0.023071 0.023596 0.061176
Eisosomes 0.000016 0.000022 0.000018 0.000023 0.000046 0.000019 0.000034 0.000033 0.000028 0.000020 0.000027 0.000028
Endoplasmic Reticulum 0.001830 0.001804 0.002385 0.001170 0.001133 0.004143 0.002694 0.001076 0.001558 0.003307 0.002558 0.001120
Endosome 0.050510 0.063272 0.019204 0.151896 0.073469 0.053632 0.120067 0.175277 0.059461 0.057079 0.075550 0.164200
Golgi 0.000858 0.002651 0.000240 0.003045 0.001150 0.001864 0.000746 0.004155 0.000972 0.002145 0.000523 0.003629
Lipid Particles 0.001445 0.001294 0.001367 0.003231 0.002980 0.001258 0.003095 0.002349 0.002044 0.001271 0.002332 0.002767
Mitochondria 0.002666 0.003939 0.002041 0.002264 0.000994 0.003352 0.001338 0.002752 0.002014 0.003562 0.001648 0.002521
Mitotic Spindle 0.000787 0.003438 0.024405 0.014604 0.000785 0.002914 0.004026 0.027689 0.000786 0.003102 0.013020 0.021490
None 0.000133 0.001222 0.004592 0.000608 0.000844 0.001007 0.002232 0.002928 0.000410 0.001084 0.003274 0.001829
Nuclear Periphery 0.000275 0.000268 0.005128 0.001290 0.000278 0.000231 0.002710 0.001400 0.000276 0.000244 0.003777 0.001348
Nuclear Periphery Foci 0.000079 0.000264 0.001477 0.001440 0.000168 0.000116 0.001553 0.001807 0.000114 0.000169 0.001520 0.001633
Nucleolus 0.000450 0.000750 0.004345 0.003079 0.000985 0.000958 0.010325 0.002780 0.000659 0.000883 0.007686 0.002922
Nucleus 0.007373 0.015829 0.341400* 0.028026 0.025624 0.013853 0.123655 0.042251 0.014489 0.014560 0.219760* 0.035512
Peroxisomes 0.000615 0.001813 0.001423 0.009634 0.001896 0.000911 0.002907 0.008602 0.001115 0.001234 0.002252 0.009091
Vacuole 0.848261* 0.701745* 0.121410 0.475734* 0.658318* 0.804427* 0.492669* 0.446587* 0.774204* 0.767710* 0.328809* 0.460395*
Vacuole Periphery 0.014593 0.017729 0.001179 0.008136 0.007556 0.023124 0.005984 0.006513 0.011850 0.021195 0.003863 0.007282

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.01 -12.42 -3.10 -2.42 -1.55 -0.59 -1.45 -2.37 -2.27 -2.18 -2.44 -6.71 -3.45 -3.06 -2.64
Bud Neck -0.86 -9.54 -8.27 -7.23 1.64 2.74 -6.42 0.88 -2.17 7.99 0.38 -12.44 -6.10 -6.41 6.64
Bud Site -6.67 -2.77 -8.31 -1.23 -6.07 -4.70 -0.87 -6.51 -3.47 -6.03 -8.47 -3.01 -10.27 -3.65 -8.39
Cell Periphery 2.79 7.50 7.69 6.10 2.32 -4.89 6.18 7.26 6.66 2.26 -2.71 8.58 9.18 7.83 3.07
Cytoplasm -13.51 -50.80 -22.99 -9.78 17.68 16.74 -2.30 -5.34 -21.95 -3.77 3.15 -35.42 -21.42 -24.20 8.74
Cytoplasmic Foci -9.36 -0.50 -15.82 -6.52 -16.00 13.58 6.73 -0.94 -15.05 -8.02 3.32 2.74 -13.25 -16.38 -16.59
Eisosomes -2.83 -4.60 -2.51 -0.48 -0.30 7.29 2.91 3.01 -9.30 0.37 4.31 -1.02 -0.67 -5.40 0.20
Endoplasmic Reticulum 0.19 -7.02 3.64 3.49 10.41 -9.89 -12.01 0.05 9.86 11.74 -8.20 -11.16 3.76 10.28 15.35
Endosome -3.24 14.06 -11.96 -9.09 -18.71 5.31 -7.59 -8.83 -12.49 -3.29 0.95 -2.99 -15.22 -15.76 -12.72
Golgi -3.09 3.37 -2.59 0.46 -4.07 -1.36 1.05 -2.57 -1.70 -4.08 -3.39 2.31 -3.86 -1.03 -5.68
Lipid Particles 0.49 -0.79 -5.40 -7.60 -6.70 3.14 -0.29 0.83 -5.96 2.53 2.74 -1.57 -3.02 -9.62 -2.60
Mitochondria -1.07 0.89 0.84 1.84 -0.08 -4.99 -2.12 -2.36 1.91 -1.60 -2.57 0.79 -0.23 2.78 -1.61
Mitotic Spindle -2.17 -9.99 -7.27 -5.44 2.39 -2.83 -5.20 -8.25 -7.38 -6.33 -3.54 -11.16 -10.94 -9.31 -3.06
None -1.46 -20.41 -6.33 0.68 16.30 -0.50 -2.92 -1.75 -1.53 -0.18 -2.09 -10.40 -2.58 -0.90 3.04
Nuclear Periphery 0.03 -21.29 -7.28 -9.26 16.98 1.09 -11.39 -5.47 -5.65 4.01 0.41 -22.23 -8.10 -8.95 13.08
Nuclear Periphery Foci -4.04 -7.61 -3.94 -3.50 0.63 2.02 -6.87 -4.82 -4.94 -0.37 -2.20 -10.19 -6.24 -6.05 0.15
Nucleolus -1.82 -9.81 -7.69 -7.26 3.84 0.24 -13.22 -6.16 -6.23 8.33 -1.92 -16.45 -9.74 -9.25 9.15
Nucleus -5.39 -56.38 -13.53 -8.40 48.93 5.67 -24.61 -7.57 -12.37 16.75 -0.06 -54.69 -15.33 -15.09 43.72
Peroxisomes -5.37 -2.48 -7.83 -6.46 -6.72 4.24 -3.01 -6.11 -7.44 -4.83 -0.90 -5.11 -10.21 -10.00 -8.13
Vacuole 15.56 104.39* 38.72* 18.85 -34.24 -15.18 17.46 15.81 33.62* 0.73 0.96 67.12* 39.94 39.12 -18.56
Vacuole Periphery -2.27 20.53 8.24 8.26 -7.00 -15.36 4.26 3.54 15.69 0.73 -10.99 17.72 9.50 17.53 -3.82
ORF
Human Ortholog
Description Hydrophilin essential in desiccation-rehydration process; cell wall protein; contains a putative GPI-attachment site
Localization
Cell Percentages vacuole (96%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YNL190W

YNL190W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ynl190w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available