ORF
Human Ortholog
Description Integral membrane protein; required for sporulation and plasma membrane sphingolipid content; similar to SUR7; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; GFP-fusion protein is more abundant at MCCs (membrane compartment occupied by Can1) in the presence of glycerol and oleate; YNL194C has a paralog, FMP45, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0.07 0.07 0.12 0 0 0 – – – 0.08 0 0 0 0 0
Bud – 0 0 0 0 0 0 0.05 0 0.06 0.06 0.06 0 0 0 – – – 0.14 0 0 0.05 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0.07 0.07 0.11 0.2 0 0 0 – – – 0.19 0 0 0.07 0 0.05
Cytoplasm – 0.96 0.99 0.99 0.95 0.92 0.81 0.75 0.74 0.57 0.56 0.55 0.97 0.97 0.94 – – – 0.18 0.79 0.74 0.61 0.68 0.67
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria – 0 0 0 0.07 0.19 0.29 0.32 0.35 0.42 0.46 0.47 0 0 0 – – – 0.08 0.06 0.1 0.13 0.13 0.08
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.15 0 0 0 0.05 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 1 1 0 0 0 3 13 9 15 15 32 0 7 9 – – – 4 5 7 6 3 8
Bud – 1 0 0 6 6 12 23 11 13 12 15 0 3 2 – – – 6 9 5 15 4 11
Bud Neck – 1 2 4 7 15 5 5 4 3 3 4 1 1 5 – – – 1 3 1 5 5 4
Bud Site – 1 1 0 0 1 1 2 2 1 0 2 0 0 0 – – – – – – – – –
Cell Periphery – 5 0 3 3 2 9 18 26 15 22 52 7 13 15 – – – 9 4 7 22 8 18
Cytoplasm – 329 274 291 307 361 352 381 260 125 117 141 303 504 437 – – – 9 284 202 195 213 239
Endoplasmic Reticulum – 1 1 0 0 0 1 0 0 1 4 1 0 0 3 – – – 1 2 0 4 4 4
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 2 1 – – – 1 3 2 1 4 3
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 – – – 1 2 1 0 2 1
Mitochondria – 11 4 8 24 73 125 162 121 91 95 121 1 0 2 – – – 4 23 26 42 41 27
Nucleus – 0 0 1 0 1 5 1 0 1 1 1 3 2 0 – – – 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 2 6 2 4 3 0 0 0 – – – 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 – – – 0 0 0 2 0 0
Peroxisomes – 0 0 0 0 0 2 7 4 3 1 5 1 1 5 – – – 0 0 0 0 0 0
SpindlePole – 2 2 1 4 4 12 11 8 9 9 10 0 4 4 – – – 0 5 4 1 3 6
Vac/Vac Membrane – 1 0 1 0 0 0 6 3 2 1 4 0 0 2 – – – 7 5 2 14 14 27
Unique Cell Count 342 278 293 322 393 433 509 349 218 208 256 313 521 464 51 360 273 320 314 358
Labelled Cell Count 353 285 309 351 463 527 631 454 281 284 392 316 537 486 51 360 273 320 314 358


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 3.9 3.0 3.1 3.1 2.8 2.7 2.9 3.0 3.0 3.2 3.8 5.2 5.0 5.2 4.7 4.7 4.8 5.7 5.4 6.0
Std Deviation (1e-4) – 1.0 1.0 1.8 1.4 0.9 1.5 1.3 1.6 1.7 2.2 2.9 3.5 2.3 1.6 1.9 1.5 1.6 3.4 1.8 5.4
Intensity Change (Log2) – – – 0.01 0.01 -0.11 -0.15 -0.09 -0.0 -0.01 0.09 0.31 0.76 0.73 0.77 0.64 0.63 0.65 0.9 0.84 0.97


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4991 -0.1176 0.2674 -0.7069 0.2817 0.0625 1.2018 2.9323 2.0741 2.6855 1.8363 1.8366 -1.8353 0.0869 -0.8227 -0.3651 -1.4648 -0.7649
Actin 0.0358 0.0125 0.0338 0.0042 0.042 0.0054 0.0397 0.0118 0.0192 0.0593 0.0162 0.0023 0.0101 0.0096 0.0011 0.0015 0.0011 0.0067
Bud 0.0026 0.0045 0.0086 0.0276 0.003 0.0077 0.0049 0.0152 0.0071 0.0039 0.0052 0.0114 0.006 0.0029 0.0021 0.0093 0.0006 0.0044
Bud Neck 0.002 0.0007 0.0013 0.0002 0.0008 0.0033 0.0042 0.002 0.001 0.0176 0.0001 0.0015 0.0003 0.0011 0.0011 0.0198 0.0146 0.001
Bud Periphery 0.0108 0.0195 0.0259 0.006 0.0077 0.033 0.0308 0.0442 0.0132 0.0551 0.0337 0.0402 0.0195 0.0111 0.0148 0.0504 0.009 0.023
Bud Site 0.0093 0.0221 0.0216 0.002 0.0025 0.0013 0.0153 0.0803 0.0278 0.0142 0.0009 0.0063 0.0112 0.0614 0.0149 0.0104 0.0155 0.0022
Cell Periphery 0.0049 0.0121 0.0182 0.0003 0.0002 0.0149 0.0084 0.0592 0.0372 0.0423 0.0322 0.0373 0.0131 0.0394 0.0372 0.0695 0.0114 0.0422
Cytoplasm 0.0438 0.0412 0.0296 0.0241 0.0132 0.0442 0.0342 0.0449 0.0444 0.0377 0.0122 0.0659 0.035 0.0775 0.0619 0.1032 0.0648 0.0715
Cytoplasmic Foci 0.0293 0.0089 0.0285 0.0088 0.0141 0.0105 0.0265 0.0039 0.0216 0.0149 0.0323 0.0082 0.0148 0.0021 0.0051 0.0043 0.0064 0.0025
Eisosomes 0.0067 0.0028 0.0013 0.0002 0.0002 0.0009 0.0014 0.0024 0.0006 0.0012 0.0005 0.0004 0.0032 0.0048 0.0007 0.0008 0.001 0.0019
Endoplasmic Reticulum 0.0064 0.0011 0.0044 0.0006 0.0047 0.0022 0.0044 0.0008 0.0021 0.0022 0.0007 0.0007 0.001 0.0007 0.0008 0.0009 0.0009 0.0028
Endosome 0.024 0.0037 0.0188 0.0038 0.0382 0.0064 0.0183 0.0005 0.0176 0.0036 0.0077 0.0032 0.0023 0.0001 0.0007 0.0005 0.0017 0.0004
Golgi 0.0101 0.0024 0.0072 0.0065 0.0369 0.0029 0.0045 0.0003 0.0083 0.0054 0.0051 0.0005 0.0051 0.0001 0.0002 0.0002 0.0001 0.0001
Lipid Particles 0.0278 0.0037 0.0068 0.0046 0.0063 0.0011 0.0075 0.0004 0.0082 0.0026 0.0117 0.0005 0.0023 0.0002 0.0005 0.0003 0.0011 0.0002
Mitochondria 0.0212 0.004 0.0158 0.0097 0.0104 0.0088 0.0083 0.0015 0.005 0.006 0.0036 0.0006 0.0019 0.0002 0.0008 0.0008 0.0005 0.0007
None 0.6932 0.853 0.7442 0.8858 0.7854 0.8478 0.7597 0.7186 0.7601 0.7114 0.7936 0.8079 0.8679 0.7861 0.8486 0.7245 0.8673 0.8352
Nuclear Periphery 0.0198 0.0003 0.0025 0.0004 0.004 0.0007 0.008 0.0003 0.0012 0.0005 0.0002 0.0006 0.0003 0.0002 0.0003 0.0003 0.0009 0.0003
Nucleolus 0.0028 0.0002 0.0023 0.0009 0.0002 0.001 0.0004 0.0011 0.0006 0.0009 0.0001 0.0002 0.0003 0.0002 0.0013 0.0005 0.0002 0.0002
Nucleus 0.0179 0.0006 0.0067 0.0035 0.0007 0.0019 0.0017 0.002 0.0008 0.001 0.0002 0.0006 0.0003 0.0004 0.0009 0.0006 0.0005 0.0007
Peroxisomes 0.0093 0.0051 0.0108 0.0093 0.004 0.0033 0.0143 0.0013 0.0107 0.0162 0.0424 0.0015 0.0023 0.0001 0.0004 0.0003 0.0004 0.0001
Punctate Nuclear 0.0067 0.0005 0.0078 0.0005 0.0011 0.0009 0.0018 0.0013 0.0013 0.0019 0.0009 0.0006 0.0024 0.0011 0.0057 0.0005 0.0008 0.0021
Vacuole 0.012 0.0008 0.003 0.0009 0.0131 0.0011 0.0036 0.0073 0.0114 0.0019 0.0003 0.0089 0.0005 0.0006 0.0007 0.0013 0.001 0.0017
Vacuole Periphery 0.0037 0.0001 0.0009 0.0003 0.0114 0.0005 0.0021 0.0006 0.0006 0.0002 0.0001 0.0007 0.0001 0 0.0001 0.0001 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2267 1701 1455 1217 2225 1864 1676 1480 4492 3565 3131 2697

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 648.63 667.37 741.20 789.44 663.11 681.30 767.21 827.38 655.80 674.65 755.12 810.26
Standard Deviation 69.63 86.82 107.59 134.70 78.71 85.29 90.66 126.39 74.62 86.31 99.74 131.56
Intensity Change Log 2 — 0.041091 0.192467 0.283434 — 0.039042 0.210373 0.319302 — 0.040056 0.201547 0.301677

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000428 0.001165 0.006954 0.007812 0.002269 0.005758 0.004470 0.009329 0.001340 0.003567 0.005625 0.008645
Bud Neck 0.028848 0.050865 0.019248 0.007029 0.027407 0.059505 0.007986 0.011445 0.028134 0.055382 0.013220 0.009453
Bud Site 0.007451 0.014464 0.040360 0.041065 0.026898 0.028915 0.025114 0.056227 0.017083 0.022020 0.032199 0.049385
Cell Periphery 0.000572 0.001446 0.005737 0.000339 0.004548 0.002087 0.003289 0.006123 0.002542 0.001781 0.004426 0.003513
Cytoplasm 0.435156* 0.378673* 0.341337* 0.440856* 0.430943* 0.346574* 0.370335* 0.445222* 0.433069* 0.361890* 0.356859* 0.443252*
Cytoplasmic Foci 0.233634* 0.235671* 0.028716 0.023653 0.202442* 0.275834* 0.009890 0.017890 0.218184* 0.256671* 0.018639 0.020491
Eisosomes 0.000822 0.001100 0.000655 0.000097 0.001366 0.001569 0.000809 0.001875 0.001091 0.001345 0.000737 0.001073
Endoplasmic Reticulum 0.000911 0.000648 0.001996 0.002007 0.001849 0.000666 0.002348 0.001354 0.001375 0.000658 0.002184 0.001649
Endosome 0.013693 0.016217 0.001637 0.010358 0.011939 0.028068 0.001260 0.001925 0.012824 0.022413 0.001435 0.005731
Golgi 0.004312 0.004081 0.000500 0.007478 0.003086 0.008723 0.000105 0.000393 0.003705 0.006508 0.000289 0.003590
Lipid Particles 0.015716 0.009209 0.006100 0.001320 0.014378 0.013535 0.000817 0.001012 0.015053 0.011471 0.003272 0.001151
Mitochondria 0.009164 0.016828 0.001439 0.006793 0.009475 0.025805 0.000614 0.001398 0.009318 0.021522 0.000997 0.003832
Mitotic Spindle 0.003872 0.003185 0.001248 0.077229 0.002092 0.005929 0.005700 0.012967 0.002990 0.004620 0.003631 0.041965
None 0.003194 0.001858 0.014099 0.007480 0.003322 0.001794 0.004766 0.005405 0.003257 0.001825 0.009103 0.006341
Nuclear Periphery 0.000362 0.000387 0.000805 0.001924 0.000564 0.000326 0.001863 0.001197 0.000462 0.000355 0.001371 0.001525
Nuclear Periphery Foci 0.000155 0.000136 0.000515 0.001453 0.000166 0.000216 0.000304 0.000465 0.000161 0.000178 0.000402 0.000911
Nucleolus 0.001382 0.001418 0.000788 0.000780 0.001519 0.001576 0.000459 0.000458 0.001450 0.001501 0.000612 0.000603
Nucleus 0.179683* 0.167798* 0.503643* 0.239435* 0.192405* 0.101619 0.528249* 0.345741* 0.185985* 0.133196* 0.516815* 0.297772*
Peroxisomes 0.009981 0.017102 0.002909 0.002871 0.008356 0.024873 0.000581 0.001587 0.009176 0.021165 0.001663 0.002166
Vacuole 0.049626 0.076531 0.021173 0.112943 0.053618 0.064204 0.030800 0.076986 0.051603 0.070086 0.026326 0.093211
Vacuole Periphery 0.001038 0.001216 0.000142 0.007077 0.001359 0.002423 0.000241 0.001000 0.001197 0.001847 0.000195 0.003742

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.51 -4.02 -2.11 -1.57 1.56 -2.60 -0.76 -2.08 0.27 -1.39 -3.14 -3.73 -3.07 -0.74 0.23
Bud Neck -7.60 4.37 14.88 17.53 5.80 -10.14 12.32 10.98 16.89 -1.34 -12.63 11.06 17.70 23.96 3.69
Bud Site -3.97 -7.95 -5.36 -2.59 3.49 -0.57 2.58 -1.62 -1.22 -3.82 -2.49 -4.01 -4.43 -2.55 -0.37
Cell Periphery -1.21 -4.06 1.24 1.94 4.54 1.94 1.82 0.88 -0.94 -0.85 1.08 -1.23 0.86 -0.11 2.06
Cytoplasm 7.02 11.57 7.36 1.37 -2.66 10.39 11.95 5.10 -4.04 -5.66 12.41 16.67 8.76 -1.96 -5.97
Cytoplasmic Foci -0.41 42.32 46.93 41.42 5.40 -10.74 44.84 42.77 47.80 -5.72 -7.99 61.70 63.39 63.03 1.37
Eisosomes -0.49 0.84 3.32 2.09 5.03 -0.38 1.87 0.71 1.02 -0.94 -0.66 2.01 1.72 2.00 -0.05
Endoplasmic Reticulum 1.46 -1.92 -7.39 -10.43 -0.83 3.88 -2.08 -0.67 -10.45 2.00 4.07 -2.95 -4.80 -14.70 0.56
Endosome -1.93 15.80 8.35 8.52 -5.02 -10.86 15.61 14.89 19.84 -2.16 -9.58 22.26 16.05 20.64 -5.40
Golgi 0.49 8.77 1.37 1.06 -2.90 -7.58 13.05 11.61 11.95 -1.56 -5.63 13.83 4.78 8.60 -3.05
Lipid Particles 6.12 7.08 16.29 12.62 4.10 0.67 18.49 18.14 14.09 -3.52 4.49 15.57 24.12 18.63 3.81
Mitochondria -4.29 8.34 4.70 7.63 -2.21 -7.47 8.13 7.60 13.08 -3.23 -8.58 11.70 8.95 15.02 -2.78
Mitotic Spindle 0.51 2.25 -8.96 -9.14 -9.66 -2.81 -2.16 -3.94 -1.53 -2.00 -1.68 -0.39 -9.61 -8.76 -9.41
None 2.05 -6.20 -2.76 -4.10 4.62 2.67 -1.72 -1.28 -4.16 -0.05 3.32 -6.39 -2.79 -5.73 4.27
Nuclear Periphery -0.92 -12.81 -13.49 -13.30 -10.42 3.86 -4.01 -12.59 -17.00 0.71 3.01 -5.26 -18.31 -19.82 -3.37
Nuclear Periphery Foci 1.22 -2.35 -5.37 -5.47 -2.14 -1.00 -7.37 -5.99 -3.40 -2.14 -0.74 -3.74 -7.27 -6.50 -2.64
Nucleolus -0.30 2.23 2.50 3.42 0.61 0.02 3.54 3.51 6.81 0.03 -0.20 4.24 4.39 7.08 0.56
Nucleus 2.05 -36.22 -14.70 -15.33 17.83 17.35 -44.84 -22.63 -34.14 16.82 13.51 -57.46 -26.79 -34.91 24.17
Peroxisomes -5.26 7.03 9.56 13.33 1.93 -9.36 11.21 9.74 14.56 -1.68 -10.59 12.49 13.64 19.49 0.91
Vacuole -8.71 10.78 -20.72 -16.21 -23.97 -3.49 1.13 -15.13 -12.81 -15.15 -8.61 6.47 -25.38 -20.34 -27.68
Vacuole Periphery -1.65 12.70 -2.22 -2.03 -3.28 -4.64 9.33 2.80 5.72 -1.46 -5.01 14.30 -1.07 0.46 -3.58
ORF
Human Ortholog
Description Integral membrane protein; required for sporulation and plasma membrane sphingolipid content; similar to SUR7; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; GFP-fusion protein is more abundant at MCCs (membrane compartment occupied by Can1) in the presence of glycerol and oleate; YNL194C has a paralog, FMP45, that arose from the whole genome duplication
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

YNL194C

YNL194C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ynl194c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available