ORF | |
---|---|
Human Ortholog | |
Description | Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | – | – | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 1 | 1 | 2 |
Bud | – | – | – | 2 | 1 | 3 | – | 14 | 8 | 11 | 6 | 7 | 2 | 0 | 0 | – | – | – | 0 | 1 | 0 | 1 | 4 | 3 |
Bud Neck | – | – | – | 1 | 2 | 0 | – | 2 | 1 | 6 | 0 | 5 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Site | – | – | – | 0 | 0 | 0 | – | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | 2 | 1 | 0 | – | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 1 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | – | – | 65 | 135 | 92 | – | 252 | 80 | 198 | 73 | 146 | 71 | 131 | 169 | – | – | – | 10 | 13 | 16 | 5 | 59 | 101 |
Endoplasmic Reticulum | – | – | – | 1 | 1 | 2 | – | 1 | 2 | 1 | 0 | 1 | 1 | 0 | 0 | – | – | – | 1 | 0 | 1 | 0 | 1 | 2 |
Endosome | – | – | – | 0 | 2 | 1 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | – | – | – | 1 | 1 | 0 | 0 | 3 | 5 |
Golgi | – | – | – | 1 | 2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | – | – | – | 1 | 0 | 0 | 1 | 3 | 3 |
Mitochondria | – | – | – | 27 | 34 | 63 | – | 99 | 94 | 114 | 82 | 89 | 1 | 6 | 3 | – | – | – | 11 | 25 | 10 | 13 | 22 | 32 |
Nucleus | – | – | – | 0 | 0 | 0 | – | 3 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | – | – | – | 0 | 0 | 0 | – | 0 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | – | – | – | 0 | 0 | 0 | – | 1 | 0 | 4 | 0 | 0 | 1 | 0 | 0 | – | – | – | 0 | 0 | 0 | 1 | 0 | 0 |
Peroxisomes | – | – | – | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | – | – | 0 | 4 | 0 | – | 10 | 2 | 11 | 5 | 4 | 0 | 1 | 0 | – | – | – | 1 | 0 | 0 | 0 | 2 | 1 |
Vac/Vac Membrane | – | – | – | 1 | 2 | 7 | – | 4 | 0 | 2 | 0 | 0 | 0 | 2 | 3 | – | – | – | 1 | 2 | 0 | 3 | 8 | 20 |
Unique Cell Count | 81 | 174 | 135 | 325 | 158 | 280 | 124 | 206 | 75 | 137 | 176 | 29 | 48 | 36 | 30 | 114 | 181 | |||||||
Labelled Cell Count | 100 | 185 | 168 | 387 | 189 | 353 | 170 | 257 | 77 | 141 | 179 | 29 | 48 | 36 | 30 | 114 | 181 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | – | – | 3.7 | 4.3 | 3.4 | – | 3.3 | 2.9 | 3.3 | 2.8 | 3.3 | 5.0 | 4.6 | 4.9 | – | – | – | – | 5.8 | 5.2 |
Std Deviation (1e-4) | – | – | – | 1.9 | 2.0 | 2.0 | – | 1.7 | 1.9 | 2.1 | 1.5 | 2.9 | 1.9 | 1.3 | 1.8 | – | – | – | – | 3.0 | 1.8 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasmic Foci | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Eisosomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Lipid Particles | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
None | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Punctate Nuclear | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | – | – | – | – | – | – | – | – | – | – |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | – |
Protein Localization | – |
Gene Expression | – |
Translational Efficiency | – |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
114 | 274 | 1160 | 1146 | 97 | 309 | 1606 | 124 | 211 | 583 | 2766 | 1270 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 620.22 | 662.67 | 598.62 | 695.16 | 685.41 | 732.26 | 655.79 | 758.62 | 650.19 | 699.55 | 631.81 | 701.36 |
Standard Deviation | 66.79 | 71.96 | 81.17 | 92.74 | 59.83 | 72.88 | 89.31 | 92.12 | 71.50 | 80.35 | 90.50 | 94.57 |
Intensity Change Log 2 | — | 0.095511 | -0.051140 | 0.164565 | — | 0.095389 | -0.063733 | 0.146410 | — | 0.095447 | -0.057737 | 0.155063 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000290 | 0.001187 | 0.000951 | 0.005790 | 0.000827 | 0.000401 | 0.001476 | 0.005948 | 0.000537 | 0.000771 | 0.001256 | 0.005805 |
Bud Neck | 0.022331 | 0.019049 | 0.038270 | 0.036411 | 0.019843 | 0.034504 | 0.033072 | 0.048311 | 0.021187 | 0.027241 | 0.035252 | 0.037573 |
Bud Site | 0.006404 | 0.023318 | 0.046613 | 0.057583 | 0.010006 | 0.018791 | 0.076623 | 0.068038 | 0.008060 | 0.020919 | 0.064038 | 0.058604 |
Cell Periphery | 0.000376 | 0.000160 | 0.000250 | 0.000365 | 0.000738 | 0.000346 | 0.000326 | 0.000542 | 0.000543 | 0.000259 | 0.000294 | 0.000382 |
Cytoplasm | 0.019296 | 0.041831 | 0.235181* | 0.219521* | 0.093585 | 0.050318 | 0.215143* | 0.168247* | 0.053448 | 0.046329 | 0.223547* | 0.214514* |
Cytoplasmic Foci | 0.247960* | 0.311402* | 0.347151* | 0.267758* | 0.351005* | 0.294365* | 0.256034* | 0.307724* | 0.295332* | 0.302372* | 0.294247* | 0.271660* |
Eisosomes | 0.000297 | 0.000108 | 0.000185 | 0.000430 | 0.000482 | 0.000305 | 0.000239 | 0.000537 | 0.000382 | 0.000212 | 0.000217 | 0.000440 |
Endoplasmic Reticulum | 0.000346 | 0.000497 | 0.001452 | 0.002151 | 0.001690 | 0.000334 | 0.001707 | 0.001086 | 0.000964 | 0.000411 | 0.001600 | 0.002047 |
Endosome | 0.049058 | 0.107620 | 0.098470 | 0.075531 | 0.059410 | 0.122658 | 0.114722 | 0.069639 | 0.053817 | 0.115590 | 0.107906 | 0.074956 |
Golgi | 0.206025* | 0.181423* | 0.031539 | 0.043403 | 0.157647* | 0.139403* | 0.067893 | 0.032111 | 0.183785* | 0.159152* | 0.052647 | 0.042301 |
Lipid Particles | 0.024721 | 0.012044 | 0.015386 | 0.010990 | 0.042714 | 0.016930 | 0.008244 | 0.009632 | 0.032993 | 0.014634 | 0.011239 | 0.010857 |
Mitochondria | 0.356362* | 0.195951* | 0.024002 | 0.043920 | 0.163458* | 0.201769* | 0.076595 | 0.027530 | 0.267681* | 0.199035* | 0.054538 | 0.042320 |
Mitotic Spindle | 0.002039 | 0.006720 | 0.001300 | 0.010351 | 0.000379 | 0.005068 | 0.008862 | 0.004631 | 0.001276 | 0.005844 | 0.005691 | 0.009792 |
None | 0.000921 | 0.000710 | 0.005885 | 0.000333 | 0.000269 | 0.001782 | 0.001244 | 0.000153 | 0.000621 | 0.001278 | 0.003191 | 0.000315 |
Nuclear Periphery | 0.000092 | 0.000065 | 0.000358 | 0.000442 | 0.000315 | 0.000315 | 0.000226 | 0.000121 | 0.000195 | 0.000197 | 0.000281 | 0.000411 |
Nuclear Periphery Foci | 0.000136 | 0.000185 | 0.001561 | 0.000538 | 0.000153 | 0.000192 | 0.000464 | 0.000277 | 0.000144 | 0.000189 | 0.000924 | 0.000512 |
Nucleolus | 0.000663 | 0.001636 | 0.005772 | 0.000458 | 0.000661 | 0.003585 | 0.001199 | 0.000285 | 0.000662 | 0.002669 | 0.003117 | 0.000441 |
Nucleus | 0.002372 | 0.002418 | 0.026143 | 0.015605 | 0.007157 | 0.008461 | 0.009555 | 0.010344 | 0.004571 | 0.005621 | 0.016512 | 0.015092 |
Peroxisomes | 0.049014 | 0.056204 | 0.036437 | 0.068124 | 0.056807 | 0.071430 | 0.025066 | 0.155802* | 0.052596 | 0.064274 | 0.029835 | 0.076684 |
Vacuole | 0.003107 | 0.021397 | 0.074495 | 0.132306 | 0.019741 | 0.019235 | 0.084029 | 0.084745 | 0.010754 | 0.020251 | 0.080031 | 0.127662 |
Vacuole Periphery | 0.008190 | 0.016073 | 0.008597 | 0.007992 | 0.013112 | 0.009808 | 0.017280 | 0.004298 | 0.010453 | 0.012752 | 0.013639 | 0.007631 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.69 | -3.52 | -6.14 | -4.39 | -5.30 | 1.15 | -1.25 | -2.76 | -3.06 | -2.37 | -0.77 | -2.49 | -6.23 | -5.79 | -5.25 |
Bud Neck | 0.33 | -1.66 | -1.47 | -3.25 | 0.58 | -2.18 | -2.48 | -2.20 | -0.95 | -1.15 | -0.96 | -2.49 | -2.68 | -2.43 | -0.65 |
Bud Site | -3.67 | -11.39 | -12.82 | -5.93 | -2.21 | -2.29 | -14.24 | -4.04 | -3.41 | 0.90 | -4.43 | -18.79 | -12.88 | -8.60 | 1.49 |
Cell Periphery | 2.80 | 1.69 | 0.22 | -3.56 | -2.05 | 1.31 | 1.40 | 0.68 | -0.91 | -1.08 | 1.96 | 1.76 | 1.15 | -1.99 | -1.62 |
Cytoplasm | -3.11 | -26.73 | -25.16 | -18.78 | 1.42 | 2.59 | -7.36 | -3.62 | -6.84 | 1.99 | 0.73 | -18.81 | -16.04 | -21.56 | 0.83 |
Cytoplasmic Foci | -2.89 | -5.12 | -0.97 | 3.25 | 8.80 | 2.26 | 4.28 | 1.49 | -0.58 | -2.66 | -0.43 | 0.11 | 1.60 | 3.07 | 3.21 |
Eisosomes | 3.77 | 2.16 | -2.20 | -10.72 | -7.33 | 2.04 | 3.77 | -0.25 | -1.70 | -2.54 | 3.33 | 4.03 | -1.02 | -5.22 | -7.32 |
Endoplasmic Reticulum | -1.02 | -4.31 | -2.60 | -2.36 | -0.94 | 2.00 | -0.03 | 0.75 | -1.16 | 1.07 | 1.72 | -1.74 | -1.52 | -2.59 | -0.64 |
Endosome | -4.74 | -5.05 | -2.63 | 3.75 | 5.14 | -5.82 | -7.78 | -0.63 | 4.82 | 5.74 | -7.38 | -8.68 | -3.10 | 6.20 | 9.00 |
Golgi | 1.01 | 7.82 | 7.30 | 12.35 | -3.48 | 0.78 | 4.01 | 5.41 | 8.98 | 4.44 | 1.45 | 8.24 | 8.90 | 15.65 | 3.60 |
Lipid Particles | 1.30 | 1.03 | 1.52 | 0.34 | 2.46 | 2.39 | 3.35 | 3.06 | 1.49 | -0.23 | 2.54 | 3.18 | 3.23 | 1.45 | 0.36 |
Mitochondria | 4.59 | 10.65 | 10.00 | 9.22 | -4.87 | -1.28 | 3.45 | 5.15 | 9.74 | 4.84 | 2.90 | 9.92 | 10.48 | 13.70 | 3.29 |
Mitotic Spindle | -2.11 | 1.36 | -4.06 | -1.23 | -4.57 | -2.53 | -5.87 | -1.81 | 0.22 | 1.70 | -3.18 | -4.95 | -4.67 | -1.71 | -2.02 |
None | 0.63 | -4.28 | 3.02 | 1.85 | 4.85 | -2.83 | -4.61 | 1.19 | 3.05 | 5.35 | -2.11 | -5.06 | 2.60 | 3.24 | 5.78 |
Nuclear Periphery | 0.45 | -3.04 | -1.49 | -1.65 | -0.33 | 0.01 | 0.53 | 1.17 | 0.85 | 1.72 | -0.02 | -0.98 | -0.97 | -0.90 | -0.60 |
Nuclear Periphery Foci | -0.69 | -5.84 | -2.08 | -1.91 | 3.72 | -0.81 | -2.59 | -1.55 | -1.04 | 1.51 | -1.02 | -6.21 | -2.20 | -1.94 | 2.38 |
Nucleolus | -1.18 | -6.47 | 0.81 | 1.47 | 7.05 | -2.74 | -1.45 | 1.77 | 3.13 | 2.95 | -2.91 | -6.19 | 1.30 | 3.30 | 7.32 |
Nucleus | -0.09 | -9.57 | -9.59 | -10.05 | 3.93 | -0.55 | -1.33 | -1.43 | -0.84 | -0.61 | -0.79 | -8.45 | -7.83 | -6.36 | 0.73 |
Peroxisomes | -0.69 | 1.46 | -1.94 | -1.29 | -6.59 | -1.28 | 3.44 | -4.22 | -3.68 | -6.33 | -1.51 | 3.63 | -2.96 | -1.58 | -10.29 |
Vacuole | -4.77 | -19.99 | -24.08 | -17.42 | -9.59 | 0.08 | -9.49 | -5.18 | -5.47 | -0.98 | -2.48 | -18.59 | -20.92 | -19.58 | -9.23 |
Vacuole Periphery | -2.62 | -0.16 | 0.20 | 3.00 | 0.53 | 1.03 | -1.31 | 2.78 | 3.41 | 8.31 | -1.07 | -1.78 | 1.61 | 3.24 | 5.51 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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