ORF
Human Ortholog
Description Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
Bud 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0.05 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 0.78 0.68 0.78 0.51 0.71 0.59 0.71 0.95 0.96 0.96 0.37 0.29 0.47 0.17 0.52 0.56
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.33 0.2 0.47 0.3 0.59 0.41 0.66 0.43 0 0 0 0.39 0.54 0.28 0.46 0.2 0.18
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.05 0 0 0 0 0 0 0 0 0 0.05 0 0.11 0.07 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2
Bud 2 1 3 14 8 11 6 7 2 0 0 0 1 0 1 4 3
Bud Neck 1 2 0 2 1 6 0 5 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 1 0 1 0 2 0 0 0
Cell Periphery 2 1 0 0 0 2 2 0 0 0 1 0 0 0 0 0 0
Cytoplasm 65 135 92 252 80 198 73 146 71 131 169 10 13 16 5 59 101
Endoplasmic Reticulum 1 1 2 1 2 1 0 1 1 0 0 1 0 1 0 1 2
Endosome 0 2 1 0 0 0 0 1 0 0 3 1 1 0 0 3 5
Golgi 1 2 0 0 0 0 0 0 1 1 0 1 0 0 1 3 3
Mitochondria 27 34 63 99 94 114 82 89 1 6 3 11 25 10 13 22 32
Nucleus 0 0 0 3 0 0 2 2 0 0 0 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 2 3 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 4 0 0 1 0 0 0 0 0 1 0 0
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 4 0 10 2 11 5 4 0 1 0 1 0 0 0 2 1
Vac/Vac Membrane 1 2 7 4 0 2 0 0 0 2 3 1 2 0 3 8 20
Unique Cell Count 81 174 135 325 158 280 124 206 75 137 176 29 48 36 30 114 181
Labelled Cell Count 100 185 168 387 189 353 170 257 77 141 179 29 48 36 30 114 181


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 4.3 3.4 3.3 2.9 3.3 2.8 3.3 5.0 4.6 4.9 5.8 5.2
Std Deviation (1e-4) 1.9 2.0 2.0 1.7 1.9 2.1 1.5 2.9 1.9 1.3 1.8 3.0 1.8
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin
Bud
Bud Neck
Bud Periphery
Bud Site
Cell Periphery
Cytoplasm
Cytoplasmic Foci
Eisosomes
Endoplasmic Reticulum
Endosome
Golgi
Lipid Particles
Mitochondria
None
Nuclear Periphery
Nucleolus
Nucleus
Peroxisomes
Punctate Nuclear
Vacuole
Vacuole Periphery

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
114 274 1160 1146 97 309 1606 124 211 583 2766 1270

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 620.22 662.67 598.62 695.16 685.41 732.26 655.79 758.62 650.19 699.55 631.81 701.36
Standard Deviation 66.79 71.96 81.17 92.74 59.83 72.88 89.31 92.12 71.50 80.35 90.50 94.57
Intensity Change Log 2 0.095511 -0.051140 0.164565 0.095389 -0.063733 0.146410 0.095447 -0.057737 0.155063

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000290 0.001187 0.000951 0.005790 0.000827 0.000401 0.001476 0.005948 0.000537 0.000771 0.001256 0.005805
Bud Neck 0.022331 0.019049 0.038270 0.036411 0.019843 0.034504 0.033072 0.048311 0.021187 0.027241 0.035252 0.037573
Bud Site 0.006404 0.023318 0.046613 0.057583 0.010006 0.018791 0.076623 0.068038 0.008060 0.020919 0.064038 0.058604
Cell Periphery 0.000376 0.000160 0.000250 0.000365 0.000738 0.000346 0.000326 0.000542 0.000543 0.000259 0.000294 0.000382
Cytoplasm 0.019296 0.041831 0.235181* 0.219521* 0.093585 0.050318 0.215143* 0.168247* 0.053448 0.046329 0.223547* 0.214514*
Cytoplasmic Foci 0.247960* 0.311402* 0.347151* 0.267758* 0.351005* 0.294365* 0.256034* 0.307724* 0.295332* 0.302372* 0.294247* 0.271660*
Eisosomes 0.000297 0.000108 0.000185 0.000430 0.000482 0.000305 0.000239 0.000537 0.000382 0.000212 0.000217 0.000440
Endoplasmic Reticulum 0.000346 0.000497 0.001452 0.002151 0.001690 0.000334 0.001707 0.001086 0.000964 0.000411 0.001600 0.002047
Endosome 0.049058 0.107620 0.098470 0.075531 0.059410 0.122658 0.114722 0.069639 0.053817 0.115590 0.107906 0.074956
Golgi 0.206025* 0.181423* 0.031539 0.043403 0.157647* 0.139403* 0.067893 0.032111 0.183785* 0.159152* 0.052647 0.042301
Lipid Particles 0.024721 0.012044 0.015386 0.010990 0.042714 0.016930 0.008244 0.009632 0.032993 0.014634 0.011239 0.010857
Mitochondria 0.356362* 0.195951* 0.024002 0.043920 0.163458* 0.201769* 0.076595 0.027530 0.267681* 0.199035* 0.054538 0.042320
Mitotic Spindle 0.002039 0.006720 0.001300 0.010351 0.000379 0.005068 0.008862 0.004631 0.001276 0.005844 0.005691 0.009792
None 0.000921 0.000710 0.005885 0.000333 0.000269 0.001782 0.001244 0.000153 0.000621 0.001278 0.003191 0.000315
Nuclear Periphery 0.000092 0.000065 0.000358 0.000442 0.000315 0.000315 0.000226 0.000121 0.000195 0.000197 0.000281 0.000411
Nuclear Periphery Foci 0.000136 0.000185 0.001561 0.000538 0.000153 0.000192 0.000464 0.000277 0.000144 0.000189 0.000924 0.000512
Nucleolus 0.000663 0.001636 0.005772 0.000458 0.000661 0.003585 0.001199 0.000285 0.000662 0.002669 0.003117 0.000441
Nucleus 0.002372 0.002418 0.026143 0.015605 0.007157 0.008461 0.009555 0.010344 0.004571 0.005621 0.016512 0.015092
Peroxisomes 0.049014 0.056204 0.036437 0.068124 0.056807 0.071430 0.025066 0.155802* 0.052596 0.064274 0.029835 0.076684
Vacuole 0.003107 0.021397 0.074495 0.132306 0.019741 0.019235 0.084029 0.084745 0.010754 0.020251 0.080031 0.127662
Vacuole Periphery 0.008190 0.016073 0.008597 0.007992 0.013112 0.009808 0.017280 0.004298 0.010453 0.012752 0.013639 0.007631

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.69 -3.52 -6.14 -4.39 -5.30 1.15 -1.25 -2.76 -3.06 -2.37 -0.77 -2.49 -6.23 -5.79 -5.25
Bud Neck 0.33 -1.66 -1.47 -3.25 0.58 -2.18 -2.48 -2.20 -0.95 -1.15 -0.96 -2.49 -2.68 -2.43 -0.65
Bud Site -3.67 -11.39 -12.82 -5.93 -2.21 -2.29 -14.24 -4.04 -3.41 0.90 -4.43 -18.79 -12.88 -8.60 1.49
Cell Periphery 2.80 1.69 0.22 -3.56 -2.05 1.31 1.40 0.68 -0.91 -1.08 1.96 1.76 1.15 -1.99 -1.62
Cytoplasm -3.11 -26.73 -25.16 -18.78 1.42 2.59 -7.36 -3.62 -6.84 1.99 0.73 -18.81 -16.04 -21.56 0.83
Cytoplasmic Foci -2.89 -5.12 -0.97 3.25 8.80 2.26 4.28 1.49 -0.58 -2.66 -0.43 0.11 1.60 3.07 3.21
Eisosomes 3.77 2.16 -2.20 -10.72 -7.33 2.04 3.77 -0.25 -1.70 -2.54 3.33 4.03 -1.02 -5.22 -7.32
Endoplasmic Reticulum -1.02 -4.31 -2.60 -2.36 -0.94 2.00 -0.03 0.75 -1.16 1.07 1.72 -1.74 -1.52 -2.59 -0.64
Endosome -4.74 -5.05 -2.63 3.75 5.14 -5.82 -7.78 -0.63 4.82 5.74 -7.38 -8.68 -3.10 6.20 9.00
Golgi 1.01 7.82 7.30 12.35 -3.48 0.78 4.01 5.41 8.98 4.44 1.45 8.24 8.90 15.65 3.60
Lipid Particles 1.30 1.03 1.52 0.34 2.46 2.39 3.35 3.06 1.49 -0.23 2.54 3.18 3.23 1.45 0.36
Mitochondria 4.59 10.65 10.00 9.22 -4.87 -1.28 3.45 5.15 9.74 4.84 2.90 9.92 10.48 13.70 3.29
Mitotic Spindle -2.11 1.36 -4.06 -1.23 -4.57 -2.53 -5.87 -1.81 0.22 1.70 -3.18 -4.95 -4.67 -1.71 -2.02
None 0.63 -4.28 3.02 1.85 4.85 -2.83 -4.61 1.19 3.05 5.35 -2.11 -5.06 2.60 3.24 5.78
Nuclear Periphery 0.45 -3.04 -1.49 -1.65 -0.33 0.01 0.53 1.17 0.85 1.72 -0.02 -0.98 -0.97 -0.90 -0.60
Nuclear Periphery Foci -0.69 -5.84 -2.08 -1.91 3.72 -0.81 -2.59 -1.55 -1.04 1.51 -1.02 -6.21 -2.20 -1.94 2.38
Nucleolus -1.18 -6.47 0.81 1.47 7.05 -2.74 -1.45 1.77 3.13 2.95 -2.91 -6.19 1.30 3.30 7.32
Nucleus -0.09 -9.57 -9.59 -10.05 3.93 -0.55 -1.33 -1.43 -0.84 -0.61 -0.79 -8.45 -7.83 -6.36 0.73
Peroxisomes -0.69 1.46 -1.94 -1.29 -6.59 -1.28 3.44 -4.22 -3.68 -6.33 -1.51 3.63 -2.96 -1.58 -10.29
Vacuole -4.77 -19.99 -24.08 -17.42 -9.59 0.08 -9.49 -5.18 -5.47 -0.98 -2.48 -18.59 -20.92 -19.58 -9.23
Vacuole Periphery -2.62 -0.16 0.20 3.00 0.53 1.03 -1.31 2.78 3.41 8.31 -1.07 -1.78 1.61 3.24 5.51
ORF
Human Ortholog
Description Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
Localization
Cell Percentages mitochondrion (67%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

YNL198C

YNL198C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ynl198c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available