Standard name
Human Ortholog
Description NADHX epimerase; catalyzes isomerization of (R)- and (S)-NADHX; homologous to AIBP in mammals and the N- terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.06 0 0 0.05 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0.05 0 0 0 0 0 0 0 0 0.05 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.97 0.97 0.99 0.95 0.91 0.87 0.82 0.68 0.65 0.59 0.7 0.99 0.99 0.99 – 0.97 0.95 0.77 0.84 0.73 0.85 0.86 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.07 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.06 0 0 0 0 0.14 0.13 0.37 0.32 0.45 0.28 0 0 0 – 0.06 0 0.13 0.06 0.12 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.09 0.08 0.11 0.12 0.11 0 0 0 – 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 2 0 0 0 0 – 0 0 1 1 4 1 2 4
Bud 0 2 0 0 1 1 1 3 8 9 9 8 0 0 0 – 0 0 0 0 1 3 8 8
Bud Neck 1 1 15 1 8 14 3 5 1 1 2 1 0 2 2 – 0 1 0 1 1 1 0 2
Bud Site 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 1 2 6 9 6 3 1 0 0 0 3 9 5 0 – 1 0 0 0 3 1 2 3
Cytoplasm 149 139 226 110 204 266 276 305 216 239 167 222 195 346 372 – 113 99 133 120 159 207 289 258
Endoplasmic Reticulum 3 0 3 0 4 3 0 0 0 1 0 2 1 2 1 – 8 6 0 0 2 1 2 1
Endosome 0 0 1 0 0 0 1 1 4 3 0 1 0 0 0 – 1 0 1 0 3 1 2 1
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 – 1 1 0 0 0 2 0 2
Mitochondria 10 8 0 1 0 11 44 47 118 119 128 88 0 0 0 – 7 2 22 7 26 6 8 12
Nucleus 0 1 1 0 2 5 3 7 7 9 3 11 0 0 0 – 0 0 0 0 0 3 0 3
Nuclear Periphery 0 0 0 0 0 0 0 2 1 2 1 1 0 0 0 – 0 0 0 0 0 0 1 2
Nucleolus 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0
SpindlePole 0 0 1 0 1 1 2 2 2 0 1 1 0 1 1 – 1 0 1 2 5 3 4 6
Vac/Vac Membrane 1 0 0 0 0 3 13 33 25 40 34 35 0 0 0 – 2 2 5 1 1 2 5 4
Unique Cell Count 159 143 233 111 214 292 319 371 316 367 285 319 196 351 374 117 104 172 143 218 244 338 320
Labelled Cell Count 164 153 250 118 229 310 346 406 384 424 347 373 205 357 376 134 111 172 143 218 244 338 320


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 3.1 4.5 4.2 5.1 4.2 4.5 4.9 4.5 4.8 4.7 5.3 4.2 4.7 4.7 4.5 5.8 5.8 5.7 6.1 6.0
Std Deviation (1e-4) 0.4 0.9 1.1 0.7 1.8 1.2 1.4 1.3 1.3 1.3 1.1 1.5 0.5 1.0 2.2 1.0 1.7 1.5 2.4 3.9 4.1
Intensity Change (Log2) – – – -0.12 0.16 -0.1 0.0 0.1 -0.03 0.09 0.05 0.21 -0.1 0.05 0.05 -0.03 0.34 0.36 0.33 0.42 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.0713 -0.7067 -0.7535 -1.11 -1.3199 -0.6442 2.6635 2.5594 2.8735 2.3103 2.5326 2.8444 -0.5471 0.1801 -0.2662 -0.0544 0.2223 -0.1563
Actin 0.0008 0.0008 0.0008 0.0016 0.0002 0.0006 0.0409 0.0049 0.0146 0.0025 0.0324 0.0009 0.0134 0.0001 0.0008 0.0003 0.0388 0.0038
Bud 0.001 0.0019 0.0041 0.0028 0.0001 0.0002 0.0037 0.007 0.0003 0.0007 0.0009 0.0007 0.0007 0.0003 0.0004 0.0001 0.0009 0.0015
Bud Neck 0.0001 0.0003 0.0002 0.0051 0.0002 0.0009 0.0024 0.0002 0.0003 0.0052 0.0006 0.0008 0.0005 0.0001 0.0002 0.0001 0.0014 0.0033
Bud Periphery 0.0014 0.0011 0.0008 0.0043 0.0001 0.0002 0.0148 0.013 0.0006 0.0011 0.0014 0.0021 0.0008 0.0002 0.0003 0.0001 0.0015 0.0032
Bud Site 0.0048 0.0033 0.003 0.0126 0.0001 0.0001 0.0119 0.0159 0.0013 0.0022 0.001 0.0004 0.0017 0.0011 0.001 0.0001 0.0023 0.0003
Cell Periphery 0.0002 0.0002 0.0001 0.0004 0.0001 0 0.0006 0.0005 0.0003 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.2143 0.2463 0.2095 0.1434 0.145 0.2767 0.0877 0.2214 0.1948 0.114 0.1529 0.2035 0.2082 0.3742 0.3005 0.2524 0.3404 0.3418
Cytoplasmic Foci 0.0203 0.0105 0.012 0.0092 0.0129 0.0181 0.0279 0.0062 0.0078 0.023 0.0124 0.0072 0.0121 0.0083 0.0072 0.0337 0.0417 0.0064
Eisosomes 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0.0005 0.0001 0.0005 0.0001 0.0001 0.0001 0.0003 0 0.0001 0.0001 0.0009 0
Endoplasmic Reticulum 0.0017 0.0026 0.0028 0.0014 0.001 0.0021 0.0039 0.0011 0.0013 0.0006 0.0003 0.0006 0.0025 0.001 0.002 0.0013 0.0026 0.0009
Endosome 0.0048 0.0038 0.0052 0.0017 0.0021 0.0038 0.0194 0.0014 0.0036 0.0128 0.001 0.0013 0.0064 0.001 0.0027 0.0098 0.0226 0.001
Golgi 0.0006 0.0003 0.0003 0.0002 0.0001 0.0002 0.0102 0.0003 0.0016 0.0073 0.0017 0.0002 0.002 0.0001 0.0003 0.0007 0.0068 0.0002
Lipid Particles 0.0026 0.0008 0.0004 0.0004 0.0003 0.0013 0.01 0.0003 0.0008 0.0025 0.0006 0.0001 0.0081 0.0001 0.0004 0.0033 0.0116 0.0001
Mitochondria 0.0022 0.0004 0.0005 0.0006 0.0003 0.0005 0.0068 0.0006 0.0108 0.0028 0.0004 0.007 0.0012 0.0001 0.0003 0.0004 0.0076 0.0004
None 0.7384 0.7147 0.7502 0.8096 0.8314 0.688 0.7157 0.7233 0.7555 0.8118 0.7909 0.7688 0.7301 0.6088 0.6777 0.6905 0.4865 0.6337
Nuclear Periphery 0.0009 0.0035 0.001 0.0012 0.0006 0.0007 0.0032 0.0007 0.0015 0.0002 0.0001 0.0006 0.0026 0.0002 0.0008 0.0016 0.0036 0.0003
Nucleolus 0.0004 0.0004 0.0003 0.0003 0.0002 0.0002 0.0099 0.0002 0.0002 0.0001 0 0.0002 0.0004 0.0002 0.0009 0.0004 0.0007 0.0001
Nucleus 0.0017 0.0039 0.0022 0.002 0.0011 0.0016 0.0048 0.0008 0.0008 0.0003 0.0003 0.0024 0.0008 0.0008 0.0014 0.001 0.0019 0.001
Peroxisomes 0.0005 0.0003 0.0004 0.0004 0.0002 0.0005 0.0149 0.0004 0.0008 0.0107 0.0017 0.001 0.0049 0 0.0004 0.0015 0.0133 0.0002
Punctate Nuclear 0.0017 0.0033 0.0045 0.0014 0.0029 0.0033 0.0037 0.0007 0.0014 0.0003 0.0005 0.0016 0.0021 0.0028 0.0012 0.0015 0.0125 0.0013
Vacuole 0.0012 0.0012 0.0013 0.0008 0.0009 0.0007 0.0053 0.0008 0.0009 0.0012 0.0004 0.0005 0.0008 0.0006 0.001 0.0007 0.0016 0.0004
Vacuole Periphery 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0.0019 0.0001 0.0004 0.0003 0.0001 0.0001 0.0003 0.0001 0.0002 0.0002 0.0009 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.9126 14.6426 5.9266 17.1239 12.5492 34.2298 15.1416 22.7101 32.7183 17.9708
Translational Efficiency 1.4192 0.6785 2.1942 1.1623 1.2284 0.6995 1.0609 1.1854 0.9656 1.3687

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1382 356 2463 1479 1563 1727 2008 1649 2945 2083 4471 3128

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 593.68 721.58 699.63 797.96 677.62 761.40 763.34 773.27 638.23 754.59 728.24 784.94
Standard Deviation 63.87 97.69 92.16 118.72 85.54 87.02 90.56 134.44 86.91 90.19 96.78 127.85
Intensity Change Log 2 — 0.281474 0.236907 0.426631 — 0.168178 0.171849 0.190496 — 0.222195 0.202596 0.305590

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000169 0.000810 0.000421 0.002130 0.000037 0.000310 0.000054 0.000189 0.000099 0.000395 0.000256 0.001107
Bud Neck 0.030227 0.041558 0.012875 0.006623 0.003352 0.047602 0.004634 0.004863 0.015963 0.046569 0.009174 0.005695
Bud Site 0.003418 0.021425 0.030131 0.035568 0.002064 0.010162 0.007536 0.017231 0.002699 0.012087 0.019983 0.025901
Cell Periphery 0.000409 0.000167 0.000393 0.000191 0.000108 0.000146 0.001267 0.000479 0.000249 0.000149 0.000785 0.000343
Cytoplasm 0.385080* 0.336479* 0.506616* 0.490712* 0.642843* 0.416915* 0.445434* 0.561414* 0.521882* 0.403168* 0.479138* 0.527984*
Cytoplasmic Foci 0.341744* 0.283737* 0.026475 0.027376 0.128673 0.270516* 0.021726 0.033084 0.228661* 0.272776* 0.024342 0.030385
Eisosomes 0.000762 0.000178 0.000101 0.000053 0.000059 0.000230 0.000832 0.000105 0.000389 0.000221 0.000429 0.000080
Endoplasmic Reticulum 0.001067 0.001282 0.001067 0.001535 0.000442 0.000337 0.000515 0.000866 0.000735 0.000498 0.000819 0.001182
Endosome 0.012198 0.027266 0.003941 0.010894 0.003750 0.015088 0.001747 0.003871 0.007715 0.017170 0.002956 0.007191
Golgi 0.008072 0.008761 0.000798 0.006339 0.000492 0.003522 0.000125 0.002052 0.004049 0.004417 0.000496 0.004079
Lipid Particles 0.027036 0.008231 0.001072 0.001532 0.002784 0.006302 0.001312 0.001430 0.014165 0.006631 0.001180 0.001478
Mitochondria 0.022764 0.015681 0.000656 0.003769 0.001034 0.010361 0.000738 0.000564 0.011231 0.011270 0.000693 0.002079
Mitotic Spindle 0.000093 0.003922 0.005210 0.059107 0.000533 0.002978 0.003892 0.012132 0.000327 0.003139 0.004618 0.034343
None 0.004493 0.003059 0.004459 0.007351 0.006962 0.002456 0.007638 0.013488 0.005803 0.002559 0.005887 0.010586
Nuclear Periphery 0.000337 0.000444 0.001095 0.001591 0.000276 0.000306 0.000827 0.000801 0.000304 0.000330 0.000975 0.001174
Nuclear Periphery Foci 0.000213 0.000618 0.000426 0.001957 0.000342 0.000332 0.000231 0.001145 0.000282 0.000380 0.000338 0.001529
Nucleolus 0.001224 0.002298 0.000604 0.000719 0.000600 0.001427 0.000523 0.000818 0.000893 0.001576 0.000568 0.000771
Nucleus 0.121389 0.122366 0.342838* 0.211542* 0.185513* 0.138298* 0.465291* 0.264380* 0.155421* 0.135575* 0.397834* 0.239397*
Peroxisomes 0.016257 0.033475 0.000776 0.002458 0.000920 0.018145 0.000623 0.000389 0.008117 0.020765 0.000707 0.001367
Vacuole 0.021335 0.083951 0.059780 0.123825 0.018828 0.053389 0.034290 0.078901 0.020005 0.058612 0.048332 0.100142
Vacuole Periphery 0.001711 0.004292 0.000265 0.004731 0.000388 0.001179 0.000764 0.001799 0.001009 0.001711 0.000489 0.003185

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.95 -3.36 -2.95 -0.59 -2.20 -7.86 -3.52 -5.01 2.96 -3.80 -7.18 -4.11 -3.47 -1.25 -2.42
Bud Neck -2.17 8.49 12.22 7.77 5.95 -17.02 -2.50 -1.58 16.25 0.32 -12.31 6.58 10.40 18.00 5.25
Bud Site -6.62 -10.84 -7.55 0.36 1.79 -9.02 -3.92 -6.28 -1.87 -3.12 -11.00 -11.44 -9.80 -2.92 0.17
Cell Periphery 6.12 1.73 7.34 2.18 6.55 -3.83 -7.80 -5.49 -4.62 5.43 5.35 -6.82 0.18 -3.74 6.64
Cytoplasm 3.28 -9.56 -2.28 -4.59 6.58 25.79 25.85 13.97 -10.59 -10.07 15.35 11.26 7.96 -8.02 -2.52
Cytoplasmic Foci 4.54 52.10 52.77 22.49 2.62 -21.34 27.43 24.18 42.49 -5.26 -6.98 50.91 49.77 49.13 -2.30
Eisosomes 8.28 9.69 10.26 7.74 2.74 -14.68 -4.90 -3.55 9.68 4.64 4.91 0.47 9.45 13.38 4.85
Endoplasmic Reticulum -0.87 -1.97 -5.60 -3.05 -5.97 1.53 -4.74 -7.69 -10.03 -4.01 2.17 -4.23 -8.40 -13.15 -6.39
Endosome -4.53 9.07 5.98 6.67 -2.42 -11.17 4.08 0.68 11.94 -3.95 -8.86 9.50 5.55 12.64 -3.80
Golgi -0.12 12.26 5.90 2.54 -2.10 -8.51 5.11 -1.79 3.34 -2.55 -0.48 12.08 4.08 3.71 -3.07
Lipid Particles 9.68 17.51 17.19 5.52 -3.18 -8.61 7.23 6.88 13.14 -0.98 9.12 17.61 17.20 13.87 -3.07
Mitochondria 1.99 10.79 10.08 3.87 -3.78 -7.84 0.90 2.00 8.47 0.91 0.13 10.58 10.05 8.81 -2.44
Mitotic Spindle -3.45 -5.03 -9.32 -7.93 -7.63 -2.89 -3.63 -5.25 -3.66 -2.56 -4.05 -6.05 -10.64 -8.75 -7.79
None 1.20 0.62 -0.53 -1.68 -1.27 4.12 0.14 -2.01 -5.55 -2.37 4.31 0.77 -1.99 -5.96 -2.94
Nuclear Periphery -1.89 -18.30 -12.73 -11.69 -6.35 -0.52 -10.54 -12.27 -13.08 0.52 -0.66 -19.58 -16.42 -15.85 -5.19
Nuclear Periphery Foci -4.19 -4.42 -4.89 -2.95 -4.02 0.08 1.02 -3.03 -2.77 -3.30 -0.95 -2.37 -5.54 -4.28 -5.08
Nucleolus -2.59 2.60 3.00 4.79 0.59 -3.96 1.63 -0.52 3.28 -1.35 -3.24 2.79 2.33 5.05 -0.46
Nucleus -0.35 -35.76 -20.46 -13.44 13.03 8.02 -36.88 -12.38 -19.59 22.59 4.19 -49.15 -22.57 -24.58 24.21
Peroxisomes -3.39 11.40 10.84 6.80 -1.85 -14.47 1.38 4.45 15.02 0.72 -8.76 11.20 10.99 15.64 -0.76
Vacuole -9.37 -19.18 -30.96 -13.61 -22.32 -16.43 -12.31 -23.64 -14.59 -14.94 -18.48 -22.82 -38.05 -24.85 -25.81
Vacuole Periphery -1.39 6.79 -0.19 1.27 -2.93 -4.34 -0.75 -2.57 -0.31 -1.30 -1.93 3.11 -1.61 0.28 -3.19
Standard name
Human Ortholog
Description NADHX epimerase; catalyzes isomerization of (R)- and (S)-NADHX; homologous to AIBP in mammals and the N- terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
Localization
Cell Percentages cytoplasm (35%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Nnr1

Nnr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nnr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available