Standard name
Human Ortholog
Description Histone chaperone; involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition; interacts physically and functionally with Chromatin Assembly Factor-1 (CAF-1)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.05 0.09 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.08 0 0 0 0 0 0 0 0 0.1 0.14 0.15 0.06 0.1 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.2 0.07 0.05 0.07 0.09 0.27 0.35 0.22 0.29 0.37 0.47 0.44 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0.94 0.95 0.96 0.9 0.89 0.82 0.81 0.77 0.77 0.76 0.73 0.74 0.89 0.84 0.79 0.85 0.86 0.86 0.96 0.93 0.94 0.89 0.77 0.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0.09 0.12 0.13 0.18 0.15 0.2 0.21 0.18 0 0 0 0 0 0 0 0 0 0 0.05 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0.06 0.13 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 3 8 5 2 4 2 11 1 0 0 0 0 0 0 0 3 1 1 0
Bud Neck 0 0 0 0 0 1 0 1 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 1 1 9 10 23 22 34 45 38 1 1 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 5 7 19 10 14 19 15 11 12 6 11 34 47 66 8 21 12 0 0 0 1 1 0
Endoplasmic Reticulum 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 3 2 0 0 0 0 0 0
Endosome 0 0 0 0 0 1 0 1 0 0 0 0 1 0 1 2 1 1 0 5 1 1 2 4
Golgi 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 0 0 1
Mitochondria 37 31 22 16 26 146 255 145 153 252 242 306 2 1 2 0 7 7 1 6 0 0 2 4
Nucleus 173 442 437 213 255 441 589 509 411 519 374 518 314 284 341 114 177 115 175 447 426 190 220 221
Nuclear Periphery 0 0 4 1 5 5 15 9 11 15 9 5 4 1 4 1 2 0 0 0 0 0 0 2
Nucleolus 3 9 10 9 26 67 98 118 83 136 106 126 2 3 3 2 4 2 0 3 3 1 13 15
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 3 1 2 2 2 2 15 1 0 0 1 0 0 1 0 3
Vac/Vac Membrane 1 3 2 1 0 2 9 3 6 7 3 7 3 7 7 10 2 2 1 11 12 13 37 60
Unique Cell Count 184 465 455 237 285 541 728 657 536 683 512 696 353 337 430 134 207 133 182 479 454 213 285 319
Labelled Cell Count 216 490 484 261 323 689 1004 829 702 981 789 1026 364 346 443 141 217 141 182 479 454 213 285 319


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 7.3 6.5 6.5 6.3 5.0 4.9 5.1 4.9 4.6 4.2 4.1 8.5 8.0 7.6 9.4 8.4 8.8 8.3 8.9 9.1
Std Deviation (1e-4) 1.0 1.6 1.2 2.2 1.9 1.5 1.4 1.5 1.4 1.3 1.2 1.1 3.1 1.9 1.9 2.1 2.0 2.3 1.6 1.9 1.7
Intensity Change (Log2) – – – 0.0 -0.05 -0.37 -0.42 -0.37 -0.41 -0.51 -0.63 -0.66 0.38 0.29 0.21 0.53 0.36 0.43 0.34 0.44 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 4.0 4.4 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4.3 1.7 1.1 1.2 0.9 0.6 0.3 -0.5 0.1 5.2 6.8 7.5 0 5.1 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.0 2.3 9.3 0 7.9 9.8 0 0 0 -3.5 -3.8 -4.0 -2.6 -0.8 0.2
Nucleus -3.2 -3.5 -7.1 -7.5 -8.5 -8.6 -9.0 -9.7 -9.5 -3.9 -5.7 -7.6 -4.5 -4.8 -4.1
Nuclear Periphery 0 0 0 0 0.7 1.5 0 0 0 0 0 0 0 0 0
Nucleolus 1.2 4.3 6.0 0 8.1 7.1 0 0 0 -1.9 -1.4 -1.9 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 4.0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.515 7.7706 7.6386 6.8685 6.3819 6.8804 5.2228 6.3088 5.7746 5.2373 5.5571 5.5028 6.3504 6.7061 6.5603 6.1105 5.6976 5.907
Actin 0.0007 0 0 0 0.0006 0 0.02 0 0.0034 0.0013 0.0019 0.0021 0.0076 0 0.0001 0 0 0.0003
Bud 0 0 0 0 0 0 0.0008 0 0.0011 0.0001 0.0016 0.0001 0.0001 0 0 0 0 0
Bud Neck 0.0001 0 0 0 0.0001 0.0003 0.0009 0 0.0003 0.0004 0.0009 0.0006 0.0005 0 0.0001 0 0.0003 0.0003
Bud Periphery 0.0001 0 0 0 0 0 0.0019 0 0.0022 0.0004 0.0051 0.0002 0.0004 0 0.0001 0 0 0.0001
Bud Site 0.0001 0 0 0 0 0 0.002 0 0.001 0.0003 0.0029 0.0002 0.0005 0 0.0001 0 0.0001 0
Cell Periphery 0 0 0 0 0 0 0.0008 0 0.0003 0.0001 0.0024 0.0001 0.0002 0 0.0001 0 0 0
Cytoplasm 0.0019 0.0003 0 0 0 0 0.0076 0 0.0007 0.0035 0.003 0.0001 0.0002 0 0.0002 0 0.0004 0
Cytoplasmic Foci 0.009 0 0 0 0.0005 0 0.007 0 0.0043 0.0262 0.0039 0.0029 0.0006 0 0.0003 0 0.0001 0
Eisosomes 0 0 0 0 0 0 0.0001 0 0 0 0.0001 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0014 0 0 0 0 0 0.0028 0 0.0008 0.0025 0.003 0.0001 0.0002 0 0.0001 0 0.0001 0.0002
Endosome 0.0065 0.0002 0 0 0.0001 0 0.0184 0 0.0026 0.0226 0.0346 0.002 0.0005 0 0.0002 0 0.0001 0.0005
Golgi 0.0007 0 0 0 0.0003 0 0.0069 0 0.0029 0.0082 0.0047 0.0033 0.0004 0 0 0 0 0
Lipid Particles 0.0069 0 0 0 0.0031 0 0.0095 0 0.0019 0.0332 0.0102 0.0027 0.0017 0 0.0008 0 0.0001 0.0001
Mitochondria 0.0005 0.0002 0.0002 0 0.0111 0.0001 0.0134 0.0001 0.0056 0.0041 0.0492 0.0033 0.0015 0.0001 0.0004 0 0.0001 0.0003
None 0.0191 0 0 0 0 0 0.0042 0 0.0003 0.0007 0.0005 0.0001 0.0002 0 0.0001 0 0.0002 0.0001
Nuclear Periphery 0.0231 0.0049 0.0053 0.0003 0.0022 0.0002 0.0177 0.0006 0.0106 0.0166 0.0078 0.0005 0.0018 0.001 0.0019 0.0003 0.0009 0.0036
Nucleolus 0.0118 0.0113 0.007 0.005 0.0408 0.0389 0.0232 0.0068 0.0095 0.0103 0.0492 0.0418 0.0204 0.0084 0.0161 0.0026 0.0535 0.0259
Nucleus 0.8888 0.9726 0.9872 0.9944 0.903 0.9601 0.823 0.9923 0.9431 0.8534 0.7722 0.9364 0.9594 0.9903 0.9778 0.9971 0.9273 0.9678
Peroxisomes 0.0004 0 0 0 0.037 0 0.0112 0 0.0008 0.0063 0.0024 0.002 0.0004 0 0 0 0 0
Punctate Nuclear 0.0277 0.0001 0.0001 0.0003 0.0009 0.0001 0.0161 0.0001 0.0065 0.0062 0.0009 0.0004 0.0017 0 0.0006 0 0.0166 0.0002
Vacuole 0.0006 0.0056 0 0 0 0.0001 0.0098 0 0.001 0.0022 0.0353 0.0007 0.001 0 0.0005 0 0.0001 0.0003
Vacuole Periphery 0.0006 0.0047 0.0001 0 0.0001 0 0.003 0 0.001 0.0015 0.0082 0.0005 0.0006 0 0.0005 0 0 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 49.2015 20.189 28.3571 28.1161 49.1511 34.6638 25.8808 21.4042 26.3268 37.1786
Translational Efficiency 0.8857 0.9662 0.6587 0.9365 0.7356 1.1944 0.6987 0.8075 0.761 0.835

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1914 610 2334 805 890 1843 124 885 2804 2453 2458 1690

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 738.10 955.63 1047.58 1036.45 801.62 882.03 1071.84 1016.11 758.26 900.33 1048.80 1025.80
Standard Deviation 107.18 156.42 147.62 196.68 117.69 138.09 128.13 157.74 114.51 146.37 146.80 177.65
Intensity Change Log 2 — 0.372636 0.505172 0.489762 — 0.137909 0.419099 0.342066 — 0.255202 0.461001 0.414756

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000288 0.000498 0.000438 0.000886 0.000230 0.000422 0.000784 0.001349 0.000269 0.000441 0.000455 0.001128
Bud Neck 0.004006 0.007289 0.016787 0.014073 0.003150 0.009013 0.003786 0.028614 0.003734 0.008584 0.016131 0.021688
Bud Site 0.000827 0.000813 0.000834 0.001911 0.000332 0.001703 0.000708 0.011003 0.000670 0.001481 0.000828 0.006672
Cell Periphery 0.000185 0.000181 0.000096 0.000055 0.000281 0.000258 0.000068 0.000271 0.000215 0.000239 0.000094 0.000168
Cytoplasm 0.002854 0.000440 0.000226 0.002837 0.000464 0.000523 0.000651 0.013186 0.002095 0.000502 0.000247 0.008256
Cytoplasmic Foci 0.001179 0.000243 0.000004 0.000117 0.000046 0.000648 0.000655 0.005926 0.000819 0.000547 0.000037 0.003159
Eisosomes 0.000025 0.000039 0.000053 0.000026 0.000025 0.000038 0.000050 0.000037 0.000025 0.000038 0.000053 0.000032
Endoplasmic Reticulum 0.006546 0.006520 0.003100 0.003776 0.005085 0.007187 0.004760 0.006239 0.006082 0.007022 0.003184 0.005066
Endosome 0.000676 0.000389 0.000076 0.000282 0.000151 0.001165 0.000706 0.007421 0.000509 0.000972 0.000108 0.004020
Golgi 0.000585 0.000252 0.000022 0.000267 0.000027 0.000415 0.000119 0.003619 0.000408 0.000375 0.000027 0.002022
Lipid Particles 0.000169 0.000062 0.000010 0.000059 0.000064 0.000779 0.000724 0.001959 0.000136 0.000601 0.000046 0.001054
Mitochondria 0.001448 0.003155 0.001233 0.003304 0.000397 0.002788 0.002516 0.007312 0.001114 0.002879 0.001298 0.005403
Mitotic Spindle 0.000662 0.003212 0.000267 0.009073 0.000351 0.002748 0.001028 0.047569 0.000564 0.002864 0.000306 0.029232
None 0.005752 0.001726 0.000616 0.002416 0.002016 0.001657 0.002811 0.007014 0.004566 0.001674 0.000727 0.004824
Nuclear Periphery 0.000616 0.000316 0.000282 0.000789 0.000424 0.000547 0.000177 0.003150 0.000555 0.000490 0.000277 0.002025
Nuclear Periphery Foci 0.000564 0.000408 0.000026 0.000384 0.000289 0.000629 0.000243 0.001944 0.000477 0.000574 0.000037 0.001201
Nucleolus 0.018689 0.010392 0.004939 0.006435 0.008912 0.009981 0.009549 0.006639 0.015586 0.010083 0.005172 0.006542
Nucleus 0.952904* 0.962778* 0.970013* 0.950861* 0.977187* 0.956630* 0.967250* 0.827217* 0.960611* 0.958159* 0.969874* 0.886112*
Peroxisomes 0.000291 0.000444 0.000093 0.000214 0.000164 0.000684 0.002034 0.001113 0.000251 0.000624 0.000190 0.000685
Vacuole 0.001200 0.000620 0.000673 0.001616 0.000309 0.001611 0.001120 0.014365 0.000917 0.001365 0.000696 0.008292
Vacuole Periphery 0.000535 0.000222 0.000212 0.000618 0.000097 0.000575 0.000262 0.004055 0.000396 0.000487 0.000215 0.002418

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.38 -4.71 -7.31 -5.02 -5.56 -4.35 -5.42 -13.37 -10.89 -4.59 -4.43 -7.07 -14.35 -11.54 -11.33
Bud Neck -3.48 -16.12 -9.79 -5.98 0.84 -5.93 -0.99 -10.33 -7.99 -9.20 -6.82 -16.61 -13.30 -9.49 -4.02
Bud Site 0.15 -0.30 -1.44 -1.51 -1.38 -3.64 -1.46 -5.96 -4.85 -5.53 -2.59 -1.33 -5.84 -4.67 -5.58
Cell Periphery 0.09 3.73 5.57 2.69 2.94 0.12 1.13 0.45 1.02 -2.71 -0.32 1.98 1.33 2.47 -1.70
Cytoplasm 4.24 4.57 0.36 -1.87 -2.04 -0.29 -0.46 -6.64 -6.62 -6.43 4.03 4.60 -4.83 -6.64 -6.84
Cytoplasmic Foci 2.62 3.37 3.12 0.94 -2.11 -2.61 -1.32 -3.74 -2.92 -2.72 1.28 3.31 -1.39 -2.45 -3.82
Eisosomes -4.00 -10.67 -1.76 3.20 9.83 -3.13 -3.72 -4.33 -1.59 1.35 -5.74 -10.67 -5.73 1.19 6.88
Endoplasmic Reticulum 0.04 9.21 6.30 3.35 -4.07 -4.24 0.27 -3.89 0.08 -4.18 -2.28 10.02 1.23 3.57 -10.00
Endosome 1.68 4.03 2.65 0.60 -2.45 -3.74 -0.89 -4.82 -3.40 -3.48 -1.65 3.84 -3.79 -2.73 -4.99
Golgi 1.16 2.06 1.29 0.24 -1.73 -3.28 -1.29 -2.50 -1.97 -2.38 0.24 2.04 -1.54 -1.80 -2.70
Lipid Particles 2.97 4.44 2.31 -0.77 -2.96 -1.63 -1.08 -2.47 -0.62 -0.69 -1.36 2.64 -2.12 -0.13 -2.57
Mitochondria -0.97 0.35 -1.58 0.00 -1.84 -3.92 -1.18 -4.54 -2.08 -1.45 -2.59 -0.70 -4.05 -1.49 -3.88
Mitotic Spindle -1.49 0.07 -2.79 -1.45 -2.80 -2.44 -0.48 -6.84 -6.32 -6.67 -2.78 -0.16 -7.31 -6.32 -7.29
None 3.99 5.61 3.60 -0.76 -3.98 0.56 -0.49 -2.60 -3.13 -1.55 4.15 5.81 0.69 -3.22 -4.81
Nuclear Periphery 1.32 1.45 -1.30 -2.55 -2.71 -0.40 0.92 -2.54 -2.50 -2.97 0.39 1.46 -2.76 -3.03 -3.46
Nuclear Periphery Foci 0.56 3.20 -0.13 -0.49 -1.62 -0.90 0.38 -2.74 -1.69 -3.35 -0.28 3.34 -1.75 -1.29 -4.00
Nucleolus 3.73 10.31 7.75 1.70 -1.60 -0.60 -0.40 1.26 2.08 1.33 4.64 10.14 7.90 2.80 -2.30
Nucleus -1.92 -5.00 0.81 2.26 4.20 5.36 1.23 13.82 11.28 9.56 0.58 -3.07 11.21 10.45 12.99
Peroxisomes -0.51 2.88 1.48 1.44 -4.14 -3.17 -1.18 -3.25 -0.03 0.83 -2.35 1.11 -1.62 1.06 -2.45
Vacuole 2.09 1.23 -1.36 -3.01 -2.35 -3.43 -2.32 -7.54 -6.81 -7.04 -1.19 0.49 -7.34 -6.87 -7.42
Vacuole Periphery 2.24 2.03 0.04 -1.10 -1.05 -3.46 -0.83 -3.17 -2.41 -2.84 -0.64 1.45 -2.64 -2.39 -3.11
Standard name
Human Ortholog
Description Histone chaperone; involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition; interacts physically and functionally with Chromatin Assembly Factor-1 (CAF-1)
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Rtt106

Rtt106


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rtt106-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available