Standard name
Human Ortholog
Description Essential serine kinase involved in the processing of 20S pre-rRNA; involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.06 0.06 0.05 0.08 0.08 0.06 0 0.05 0 0.05 0.05 0.05 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.98 0.98 0.95 0.92 0.71 0.69 0.71 0.58 0.49 0.63 0.97 0.99 0.99 0.97 0.97 0.96 0.97 0.93 0.89 0.96 0.96 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0.05 0.06 0.1 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.07 0.14 0.35 0.32 0.36 0.5 0.58 0.41 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 1 2 0 1 2 0 1 1 0 0 0 0 0 0 0 0 0 2 0
Bud 2 0 0 0 1 1 7 0 3 7 5 4 0 0 0 0 0 1 0 0 1 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 6 5 6 5 8 8 17 13 13 24 16 15 8 21 18 11 7 6 0 0 1 0 0 0
Cytoplasm 312 172 167 127 233 195 205 142 178 177 103 158 207 451 481 217 150 128 305 169 150 266 566 500
Endoplasmic Reticulum 3 0 3 1 3 4 12 9 12 17 21 8 5 7 7 5 7 6 5 3 6 2 4 7
Endosome 0 0 0 0 1 0 0 0 0 0 0 1 1 0 0 0 0 0 0 2 0 0 1 4
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 1 0 0 2
Mitochondria 2 0 0 3 18 29 100 66 90 154 122 101 0 0 0 3 2 3 1 1 1 1 4 2
Nucleus 1 0 1 1 2 2 2 1 0 2 1 1 1 1 2 1 1 1 0 0 0 1 2 0
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 1 5 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Vac/Vac Membrane 0 0 0 0 0 0 11 8 7 11 1 3 0 0 1 1 0 1 1 2 2 2 4 3
Unique Cell Count 317 174 170 129 246 211 287 207 249 307 209 249 213 456 488 224 155 133 317 182 168 279 593 529
Labelled Cell Count 327 178 177 137 266 240 356 240 304 394 270 297 223 480 510 239 168 146 317 182 168 279 593 529


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.4 15.3 12.5 10.6 9.3 8.0 7.8 7.8 7.3 7.1 6.6 7.1 13.4 13.2 12.6 20.0 19.7 20.4 14.0 14.2 13.8
Std Deviation (1e-4) 1.6 2.1 1.7 1.5 1.3 1.5 1.4 1.4 1.4 1.3 1.1 1.4 2.2 2.0 1.8 5.3 4.9 5.2 1.8 2.2 2.3
Intensity Change (Log2) – – – -0.23 -0.43 -0.64 -0.68 -0.67 -0.76 -0.8 -0.91 -0.82 0.11 0.08 0.02 0.68 0.66 0.71 0.17 0.19 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 -0.2 0.1 1.1 1.2 0.8 1.8 1.7 1.1 0.1 0.6 0.1 0.7 0.5 0.4
Cytoplasm 0.1 -1.8 -2.6 -7.1 -7.4 -7.1 -9.5 -10.5 -8.4 -0.7 0.7 0.3 -0.9 -0.8 -1.1
Endoplasmic Reticulum 0 0 0 1.4 1.4 1.7 2.0 3.3 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 3.6 5.0 8.7 8.1 8.8 11.2 12.1 9.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin – – – – – – 0.0757 0.0094 0.0368 0.0594 0.0042 0.0029 0.0027 0.0015 0.009 0.0015 0.0027 0.0069
Bud – – – – – – 0.0012 0.0004 0.0055 0.0005 0.0006 0.003 0.0006 0.0001 0.0025 0.0039 0.0003 0.0014
Bud Neck – – – – – – 0.0015 0.0003 0.0007 0.001 0.0007 0.0007 0.006 0.0002 0.0004 0.0007 0.0004 0.0009
Bud Periphery – – – – – – 0.0011 0.0002 0.0033 0.0003 0.0008 0.0014 0.0006 0 0.0007 0.0017 0.0005 0.0004
Bud Site – – – – – – 0.0035 0.0051 0.004 0.0046 0.0007 0.0006 0.0017 0.0002 0.0031 0.0018 0.0005 0.0001
Cell Periphery – – – – – – 0.0008 0.0003 0.0002 0.0001 0.0001 0.0002 0.0003 0.0001 0.0001 0.0007 0.0001 0.0001
Cytoplasm – – – – – – 0.6818 0.949 0.8774 0.813 0.6124 0.8971 0.7885 0.9768 0.9135 0.881 0.777 0.9199
Cytoplasmic Foci – – – – – – 0.0374 0.0051 0.0137 0.0272 0.0518 0.0101 0.033 0.0027 0.0098 0.0137 0.0189 0.0145
Eisosomes – – – – – – 0.0005 0 0.0002 0.0001 0.0001 0 0 0.0002 0.0001 0 0.0001 0
Endoplasmic Reticulum – – – – – – 0.0508 0.0049 0.0061 0.005 0.005 0.0137 0.0239 0.0091 0.0052 0.0076 0.0043 0.0109
Endosome – – – – – – 0.0539 0.0052 0.0203 0.0401 0.1057 0.0146 0.0694 0.0032 0.0185 0.0482 0.0814 0.0229
Golgi – – – – – – 0.0162 0.0012 0.0108 0.0187 0.0503 0.0027 0.0122 0.0005 0.008 0.0113 0.0197 0.0083
Lipid Particles – – – – – – 0.0104 0.0004 0.0044 0.0022 0.023 0.001 0.0099 0.0001 0.0038 0.0006 0.011 0.003
Mitochondria – – – – – – 0.0053 0.0002 0.0039 0.0036 0.0504 0.0233 0.0171 0.0001 0.0062 0.0071 0.0444 0.0046
None – – – – – – 0.0127 0.0006 0.0007 0.0005 0.0004 0.0035 0.0024 0.001 0.0011 0.0014 0.0071 0.0009
Nuclear Periphery – – – – – – 0.017 0.0005 0.0007 0.0008 0.0105 0.0017 0.0063 0.0011 0.0009 0.0015 0.0078 0.0005
Nucleolus – – – – – – 0.0009 0.0001 0.0001 0 0.0002 0.0002 0.0004 0 0.0001 0.0002 0.0012 0.0001
Nucleus – – – – – – 0.0057 0.0152 0.0008 0.0009 0.0027 0.0176 0.0027 0.001 0.0011 0.0015 0.0055 0.0008
Peroxisomes – – – – – – 0.0055 0.0003 0.0078 0.0163 0.0248 0.0005 0.0092 0 0.008 0.0003 0.0016 0.0003
Punctate Nuclear – – – – – – 0.0102 0.0001 0.0003 0.0003 0.0004 0.0019 0.0011 0.0001 0.0003 0.0002 0.0058 0.0001
Vacuole – – – – – – 0.0062 0.0014 0.0018 0.0042 0.029 0.0022 0.0094 0.0018 0.0064 0.0124 0.0063 0.0023
Vacuole Periphery – – – – – – 0.0019 0.0002 0.0005 0.0012 0.0261 0.0012 0.0023 0.0002 0.0011 0.0026 0.0032 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 51.3713 29.3289 36.0812 33.5479 42.5378 30.2017 26.3378 18.698 26.6687 38.6034
Translational Efficiency 1.6016 2.1086 1.4742 1.2647 1.2988 2.0038 2.0163 2.0961 1.5723 1.507

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1996 289 1402 1706 1867 2159 1790 159 3863 2448 3192 1865

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1104.71 1095.45 1418.97 1647.77 1111.02 1190.30 1527.42 1677.51 1107.76 1179.10 1479.79 1650.31
Standard Deviation 156.33 276.42 197.43 252.78 156.30 163.80 217.49 218.36 156.35 183.36 215.74 250.17
Intensity Change Log 2 — -0.012144 0.361176 0.576847 — 0.099440 0.459212 0.594437 — 0.044886 0.411166 0.585694

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000139 0.000467 0.000284 0.000700 0.000200 0.000198 0.000370 0.000474 0.000168 0.000230 0.000332 0.000680
Bud Neck 0.000422 0.001202 0.001017 0.001397 0.000435 0.000452 0.000675 0.003085 0.000428 0.000541 0.000826 0.001541
Bud Site 0.014291 0.035585 0.011189 0.019066 0.016004 0.015670 0.010248 0.021611 0.015119 0.018021 0.010661 0.019283
Cell Periphery 0.000183 0.000387 0.000260 0.005479 0.000222 0.000141 0.000297 0.001210 0.000202 0.000170 0.000281 0.005115
Cytoplasm 0.833305* 0.799903* 0.865433* 0.863644* 0.784383* 0.803083* 0.925312* 0.722354* 0.809661* 0.802708* 0.899011* 0.851598*
Cytoplasmic Foci 0.103565 0.092256 0.035830 0.011085 0.131301 0.108251 0.019660 0.049273 0.116970 0.106363 0.026762 0.014341
Eisosomes 0.000006 0.000008 0.000004 0.000006 0.000007 0.000006 0.000003 0.000005 0.000007 0.000006 0.000003 0.000006
Endoplasmic Reticulum 0.002741 0.001447 0.001000 0.000812 0.007218 0.003457 0.000291 0.000303 0.004905 0.003220 0.000602 0.000769
Endosome 0.004110 0.012440 0.003758 0.001886 0.005625 0.007772 0.001286 0.009037 0.004842 0.008323 0.002372 0.002496
Golgi 0.011137 0.007162 0.002398 0.007836 0.011712 0.011513 0.002670 0.004424 0.011415 0.011000 0.002550 0.007545
Lipid Particles 0.000719 0.001416 0.002933 0.010091 0.000866 0.000741 0.002581 0.007156 0.000790 0.000821 0.002736 0.009841
Mitochondria 0.000513 0.000104 0.000030 0.000315 0.000681 0.001159 0.000175 0.000006 0.000594 0.001034 0.000112 0.000289
Mitotic Spindle 0.000229 0.004397 0.001352 0.001447 0.001499 0.001490 0.000553 0.001024 0.000843 0.001833 0.000904 0.001411
None 0.000642 0.000476 0.000788 0.000197 0.000469 0.000254 0.000576 0.000100 0.000558 0.000280 0.000669 0.000188
Nuclear Periphery 0.000015 0.000040 0.000064 0.000069 0.000030 0.000016 0.000014 0.000038 0.000022 0.000019 0.000036 0.000066
Nuclear Periphery Foci 0.000324 0.001635 0.002513 0.001738 0.000366 0.000351 0.001520 0.003487 0.000345 0.000502 0.001956 0.001887
Nucleolus 0.000068 0.000119 0.000067 0.000089 0.000048 0.000043 0.000055 0.000137 0.000058 0.000052 0.000060 0.000093
Nucleus 0.002691 0.003882 0.009392 0.003655 0.002258 0.002507 0.006602 0.004310 0.002481 0.002670 0.007828 0.003711
Peroxisomes 0.000287 0.000855 0.000189 0.000382 0.000273 0.000389 0.000206 0.000881 0.000280 0.000444 0.000199 0.000425
Vacuole 0.016216 0.032042 0.060086 0.066029 0.021866 0.032654 0.026315 0.169337 0.018947 0.032581 0.041148 0.074837
Vacuole Periphery 0.008397 0.004176 0.001412 0.004078 0.014537 0.009853 0.000590 0.001746 0.011364 0.009183 0.000951 0.003879

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.46 -6.90 -6.20 -1.93 -4.22 0.08 -4.68 -3.26 -3.25 -0.58 -2.68 -7.33 -6.16 -5.32 -3.70
Bud Neck -3.69 -9.90 -9.46 -1.28 -2.48 -0.47 -6.06 -7.59 -7.55 -6.71 -2.85 -11.37 -11.36 -10.03 -6.26
Bud Site -6.22 2.05 -3.82 4.53 -5.05 0.29 5.40 -2.46 -2.58 -4.72 -2.98 5.21 -3.82 -1.24 -7.05
Cell Periphery -3.79 -3.00 -10.69 -10.22 -10.53 6.15 -3.18 -4.10 -4.51 -3.72 2.67 -4.52 -10.79 -10.87 -10.61
Cytoplasm 2.47 -2.58 -3.82 -4.25 -1.03 -2.57 -22.50 3.88 4.76 10.34 1.22 -17.84 -5.95 -6.32 8.09
Cytoplasmic Foci 1.50 18.10 27.55 11.35 14.80 4.82 29.76 12.16 9.01 -5.26 2.81 34.33 39.26 29.98 10.39
Eisosomes -1.57 4.05 0.20 1.70 -3.75 2.18 7.48 1.82 0.02 -4.00 1.15 8.12 1.18 0.15 -4.91
Endoplasmic Reticulum 1.77 3.50 3.64 1.11 0.60 3.93 8.31 8.33 6.69 0.18 2.65 8.97 8.07 5.31 -0.83
Endosome -3.72 0.55 6.79 4.72 5.97 -3.77 12.43 -1.69 -0.55 -3.99 -6.53 9.59 7.34 10.60 -0.18
Golgi 3.98 18.33 2.83 -0.31 -4.35 0.23 13.70 5.52 4.86 -0.90 0.55 21.14 3.66 2.84 -4.26
Lipid Particles -2.61 -10.56 -11.71 -10.32 -8.45 1.81 -5.40 -6.03 -6.14 -4.22 -0.56 -9.69 -12.31 -12.26 -9.14
Mitochondria 4.41 5.34 1.29 -1.44 -1.99 -1.41 2.70 4.59 3.77 1.26 -1.56 4.45 2.10 2.55 -1.21
Mitotic Spindle -1.48 -4.46 -2.15 0.91 0.06 0.01 1.62 0.48 0.46 -0.92 -1.57 -0.99 -1.25 0.11 -0.86
None 0.99 -0.32 4.16 1.96 1.90 3.10 -0.57 5.66 3.67 3.34 3.97 -0.48 5.71 2.31 3.02
Nuclear Periphery -1.62 -5.26 -1.54 -0.83 -0.03 1.93 1.75 -1.78 -3.27 -3.16 0.70 -3.29 -1.43 -1.53 -0.87
Nuclear Periphery Foci -2.96 -11.29 -8.12 -0.26 4.65 0.15 -11.21 -5.52 -5.51 -3.27 -1.33 -15.01 -11.44 -9.85 1.25
Nucleolus -1.41 -0.19 -1.55 0.84 -2.25 0.97 -1.47 -5.17 -5.61 -4.87 0.83 -0.55 -3.76 -5.13 -4.41
Nucleus -1.50 -15.10 -2.51 -0.10 10.08 -1.71 -16.11 -4.05 -3.60 3.96 -0.97 -21.21 -3.58 -3.13 9.53
Peroxisomes -3.47 4.16 -1.21 2.60 -2.24 -4.38 2.50 -4.95 -4.00 -5.45 -5.78 4.52 -1.99 0.23 -2.96
Vacuole -3.80 -14.49 -18.10 -7.51 -0.26 -5.41 -4.46 -9.06 -8.47 -8.59 -8.50 -14.65 -19.05 -13.97 -9.17
Vacuole Periphery 3.98 11.10 5.82 0.19 -5.54 3.57 12.59 11.32 10.83 -3.41 2.47 16.61 10.17 7.17 -6.85
Standard name
Human Ortholog
Description Essential serine kinase involved in the processing of 20S pre-rRNA; involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Rio2

Rio2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rio2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available