Standard name | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 1 | 2 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 3 | – | 3 | 4 | 2 | 4 | 14 | 12 |
Bud | 3 | 4 | 1 | 7 | 3 | 4 | 5 | – | 14 | 11 | 16 | 10 | 7 | 7 | 3 | 1 | 1 | – | 0 | 0 | 0 | 0 | 3 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | – | 3 | 0 | 0 | 1 | 5 | 7 |
Bud Site | 2 | 0 | 0 | 0 | 0 | 2 | 1 | – | 3 | 3 | 4 | 2 | 1 | 1 | 0 | 1 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 3 | 6 | 1 | 6 | 3 | 0 | 2 | – | 3 | 0 | 3 | 2 | 8 | 3 | 5 | 2 | 0 | – | 1 | 0 | 0 | 0 | 1 | 1 |
Cytoplasm | 5 | 25 | 6 | 19 | 27 | 33 | 34 | – | 28 | 30 | 32 | 34 | 16 | 46 | 59 | 0 | 4 | – | 0 | 4 | 2 | 0 | 3 | 2 |
Endoplasmic Reticulum | 1 | 7 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 3 | 2 | 3 | 0 | 1 | – | 0 | 0 | 1 | 1 | 2 | 3 |
Endosome | 15 | 4 | 8 | 12 | 47 | 3 | 36 | – | 11 | 10 | 20 | 15 | 43 | 44 | 86 | 4 | 1 | – | 31 | 20 | 6 | 25 | 42 | 30 |
Golgi | 2 | 3 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 3 | – | 7 | 6 | 2 | 9 | 19 | 15 |
Mitochondria | 196 | 141 | 15 | 52 | 47 | 88 | 103 | – | 158 | 158 | 168 | 179 | 142 | 99 | 64 | 2 | 3 | – | 180 | 142 | 67 | 152 | 250 | 179 |
Nucleus | 1 | 1 | 0 | 0 | 0 | 4 | 9 | – | 6 | 1 | 8 | 6 | 0 | 2 | 0 | 1 | 1 | – | 0 | 1 | 0 | 1 | 0 | 1 |
Nuclear Periphery | 2 | 0 | 0 | 1 | 0 | 0 | 1 | – | 1 | 1 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | – | 6 | 2 | 0 | 3 | 4 | 1 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 3 | 1 | – | 0 | 4 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 4 | 2 |
Peroxisomes | 4 | 4 | 3 | 17 | 25 | 1 | 13 | – | 8 | 5 | 7 | 4 | 35 | 35 | 68 | 0 | 0 | – | 15 | 1 | 3 | 21 | 37 | 16 |
SpindlePole | 12 | 2 | 5 | 21 | 23 | 11 | 38 | – | 19 | 30 | 10 | 23 | 12 | 20 | 10 | 6 | 5 | – | 6 | 2 | 3 | 2 | 8 | 5 |
Vac/Vac Membrane | 22 | 19 | 2 | 9 | 20 | 7 | 25 | – | 26 | 17 | 22 | 14 | 27 | 15 | 29 | 2 | 4 | – | 3 | 13 | 2 | 4 | 9 | 16 |
Unique Cell Count | 228 | 176 | 40 | 109 | 145 | 126 | 198 | 203 | 214 | 221 | 231 | 217 | 222 | 265 | 17 | 19 | 265 | 206 | 96 | 233 | 412 | 299 | ||
Labelled Cell Count | 270 | 217 | 44 | 146 | 195 | 156 | 269 | 277 | 271 | 294 | 298 | 296 | 276 | 329 | 23 | 26 | 265 | 206 | 96 | 233 | 412 | 299 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 4.7 | 6.6 | 5.6 | 6.2 | 4.3 | 5.3 | – | 4.7 | 5.0 | 4.7 | 4.7 | 5.2 | 5.1 | 5.9 | 6.2 | 6.9 | – | 4.9 | 5.1 | 5.6 |
Std Deviation (1e-4) | 0.7 | 1.0 | 2.6 | 2.2 | 2.1 | 2.5 | 2.2 | – | 2.4 | 2.5 | 2.0 | 2.5 | 1.1 | 1.5 | 2.0 | 1.3 | 1.4 | – | 1.2 | 1.4 | 2.5 |
Intensity Change (Log2) | – | – | – | -0.23 | -0.08 | -0.6 | -0.32 | – | -0.47 | -0.38 | -0.49 | -0.47 | -0.35 | -0.36 | -0.17 | -0.09 | 0.06 | – | -0.43 | -0.37 | -0.24 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.7883 | -0.6285 | -0.8615 | -0.9639 | -0.3961 | -0.6709 | 0.9655 | 1.0263 | 1.1267 | 0.7641 | 1.153 | 0.8421 | 3.8145 | 3.7675 | 3.5819 | 3.7568 | 3.8775 | 3.6817 |
Actin | 0.0424 | 0.0128 | 0.015 | 0.0063 | 0.0404 | 0.015 | 0.053 | 0.0178 | 0.0283 | 0.0435 | 0.0412 | 0.0095 | 0.0665 | 0.0126 | 0.0419 | 0.0653 | 0.0586 | 0.0302 |
Bud | 0.0013 | 0.0065 | 0.0039 | 0.0008 | 0.0018 | 0.0007 | 0.0011 | 0.002 | 0.0014 | 0.0025 | 0.0017 | 0.0006 | 0.0011 | 0.0012 | 0.0016 | 0.003 | 0.0016 | 0.0008 |
Bud Neck | 0.0023 | 0.0038 | 0.0017 | 0.0011 | 0.001 | 0.0043 | 0.0049 | 0.0012 | 0.0013 | 0.0015 | 0.0007 | 0.0034 | 0.0021 | 0.0013 | 0.0032 | 0.001 | 0.0007 | 0.0043 |
Bud Periphery | 0.0022 | 0.0104 | 0.0035 | 0.0018 | 0.0027 | 0.0017 | 0.0021 | 0.004 | 0.0026 | 0.0061 | 0.0034 | 0.0012 | 0.0024 | 0.0024 | 0.0055 | 0.0144 | 0.0063 | 0.002 |
Bud Site | 0.0101 | 0.0267 | 0.0154 | 0.0011 | 0.0045 | 0.0009 | 0.0118 | 0.026 | 0.0062 | 0.0017 | 0.0015 | 0.0013 | 0.0073 | 0.0071 | 0.0099 | 0.0032 | 0.0024 | 0.0018 |
Cell Periphery | 0.001 | 0.0023 | 0.0009 | 0.0008 | 0.0003 | 0.0008 | 0.0024 | 0.0038 | 0.0008 | 0.0009 | 0.0006 | 0.0009 | 0.0013 | 0.0011 | 0.0032 | 0.001 | 0.0011 | 0.0012 |
Cytoplasm | 0.0049 | 0.0446 | 0.0079 | 0.0102 | 0.0093 | 0.0136 | 0.026 | 0.0231 | 0.0204 | 0.0363 | 0.023 | 0.008 | 0.0142 | 0.0124 | 0.0161 | 0.0064 | 0.0071 | 0.0189 |
Cytoplasmic Foci | 0.0885 | 0.0929 | 0.0656 | 0.0566 | 0.0798 | 0.0576 | 0.0801 | 0.0893 | 0.0654 | 0.0588 | 0.0499 | 0.0652 | 0.0725 | 0.112 | 0.0608 | 0.0468 | 0.0687 | 0.0555 |
Eisosomes | 0.0024 | 0.0013 | 0.0009 | 0.0003 | 0.0003 | 0.0009 | 0.002 | 0.0006 | 0.001 | 0.0007 | 0.0007 | 0.0005 | 0.0024 | 0.0011 | 0.0018 | 0.0009 | 0.0011 | 0.0006 |
Endoplasmic Reticulum | 0.0096 | 0.0071 | 0.0011 | 0.0027 | 0.0015 | 0.0028 | 0.0055 | 0.0018 | 0.0063 | 0.0031 | 0.0049 | 0.0022 | 0.0062 | 0.0046 | 0.0026 | 0.0004 | 0.0019 | 0.0011 |
Endosome | 0.1143 | 0.1034 | 0.0964 | 0.1055 | 0.1812 | 0.0866 | 0.1862 | 0.1484 | 0.114 | 0.1281 | 0.1731 | 0.1299 | 0.1433 | 0.1416 | 0.0822 | 0.0408 | 0.1399 | 0.0754 |
Golgi | 0.0926 | 0.0833 | 0.0539 | 0.0702 | 0.1206 | 0.0708 | 0.1118 | 0.1155 | 0.0844 | 0.078 | 0.0748 | 0.1301 | 0.0947 | 0.0992 | 0.0769 | 0.0499 | 0.0734 | 0.076 |
Lipid Particles | 0.0631 | 0.038 | 0.0279 | 0.0359 | 0.0708 | 0.0344 | 0.0711 | 0.0119 | 0.0187 | 0.007 | 0.0461 | 0.0421 | 0.0361 | 0.0121 | 0.0143 | 0.0146 | 0.0226 | 0.0146 |
Mitochondria | 0.4123 | 0.4294 | 0.6058 | 0.6527 | 0.4009 | 0.6298 | 0.3376 | 0.4614 | 0.5439 | 0.4848 | 0.4917 | 0.5066 | 0.4385 | 0.4777 | 0.5425 | 0.6796 | 0.4492 | 0.5973 |
None | 0.0409 | 0.0611 | 0.0283 | 0.0068 | 0.0014 | 0.0153 | 0.0139 | 0.0311 | 0.0269 | 0.0462 | 0.0028 | 0.0083 | 0.0272 | 0.0281 | 0.0493 | 0.0159 | 0.0721 | 0.0295 |
Nuclear Periphery | 0.0132 | 0.0023 | 0.0021 | 0.0051 | 0.0197 | 0.0008 | 0.0092 | 0.0007 | 0.0046 | 0.0006 | 0.0066 | 0.0015 | 0.0036 | 0.0021 | 0.0013 | 0.0002 | 0.0019 | 0.0002 |
Nucleolus | 0.0027 | 0.0013 | 0.0022 | 0.0011 | 0.0009 | 0.0009 | 0.0012 | 0.0014 | 0.0005 | 0.0003 | 0.0003 | 0.0004 | 0.001 | 0.0004 | 0.0007 | 0.0004 | 0.0007 | 0.0003 |
Nucleus | 0.0017 | 0.0015 | 0.001 | 0.0017 | 0.0016 | 0.0008 | 0.0035 | 0.0018 | 0.0009 | 0.0005 | 0.0017 | 0.0007 | 0.0014 | 0.0005 | 0.0007 | 0.0003 | 0.0007 | 0.0003 |
Peroxisomes | 0.0737 | 0.0568 | 0.0565 | 0.0254 | 0.047 | 0.0515 | 0.0588 | 0.0427 | 0.0601 | 0.0899 | 0.0623 | 0.079 | 0.0609 | 0.0731 | 0.0723 | 0.0507 | 0.0822 | 0.0819 |
Punctate Nuclear | 0.0074 | 0.0007 | 0.0017 | 0.0046 | 0.0027 | 0.0019 | 0.0049 | 0.0011 | 0.0019 | 0.0004 | 0.0009 | 0.0008 | 0.0078 | 0.0003 | 0.001 | 0.0005 | 0.0011 | 0.0003 |
Vacuole | 0.0072 | 0.009 | 0.0052 | 0.0048 | 0.0056 | 0.0047 | 0.0085 | 0.0098 | 0.0059 | 0.0051 | 0.0073 | 0.0041 | 0.0057 | 0.0049 | 0.0092 | 0.0032 | 0.0038 | 0.0046 |
Vacuole Periphery | 0.0063 | 0.0048 | 0.0032 | 0.0046 | 0.0058 | 0.0042 | 0.0046 | 0.0046 | 0.0046 | 0.004 | 0.0049 | 0.0037 | 0.0038 | 0.004 | 0.0029 | 0.0015 | 0.003 | 0.0034 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.2642 | 18.3512 | 12.4826 | 14.0628 | 19.962 | 10.2585 | 22.0339 | 17.2195 | 17.9769 | 17.7352 |
Translational Efficiency | 1.2458 | 0.5648 | 0.7433 | 0.9336 | 0.394 | 1.1926 | 0.4547 | 0.8535 | 0.8415 | 0.5802 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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