Standard name
Human Ortholog
Description Nitrogen catabolite repression transcriptional regulator; inhibits GLN3 transcription in good nitrogen source; role in sequestering Gln3p and Gat1p to the cytoplasm; has glutathione peroxidase activity and can mutate to acquire GST activity; self-assembly under limited nitrogen conditions creates [URE3] prion and releases catabolite repression

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.05 0.05 0 0.06 0 0.06 0 0.05 0.05 0.07 0 0 0.06 0.1 0.07 0.1 0 0 0 0 0 0
Cytoplasm 0.98 0.96 0.97 0.94 0.86 0.84 0.72 0.72 0.74 0.63 0.64 0.69 0.97 0.99 0.96 0.84 0.86 0.87 0.94 0.93 0.91 0.85 0.89 0.89
Endoplasmic Reticulum 0 0 0 0 0.07 0.05 0 0.07 0.05 0 0.06 0.06 0 0 0 0 0 0 0 0 0 0.06 0.05 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.11 0.27 0.17 0.24 0.36 0.34 0.2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.07 0 0.06 0 0.05 0 0 0 0.13 0.11 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 3 0 5 6 6 4 4 11 0 0 1 1 0 4 0 0 2 0 0 1
Bud 0 0 0 0 0 0 0 5 1 3 6 4 0 0 0 0 0 1 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 10 11 10 14 11 10 20 7 8 15 20 3 7 15 43 29 49 0 0 1 2 0 1
Cytoplasm 104 329 237 180 279 162 179 224 146 111 203 193 222 223 255 364 354 442 120 328 230 155 285 291
Endoplasmic Reticulum 0 9 5 7 24 9 11 21 10 5 20 18 4 3 7 13 13 17 2 8 9 10 16 11
Endosome 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0
Golgi 0 0 0 0 2 1 0 1 0 0 0 0 0 2 1 2 2 1 0 1 0 1 1 1
Mitochondria 2 3 0 5 10 21 66 53 47 64 107 56 0 0 0 4 9 18 0 2 2 4 1 5
Nucleus 0 0 2 1 2 0 4 0 3 3 1 1 0 1 1 2 5 5 0 0 0 2 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 2 2 0 1 0 0 0 0 0 1 0 1 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 2 2 4 8 7 11 22 2 10 13 13 4 0 0 56 47 35 0 5 5 3 8 6
Unique Cell Count 106 343 244 191 323 194 249 312 197 176 319 281 229 226 265 434 414 508 128 354 254 182 320 326
Labelled Cell Count 108 353 257 208 342 211 286 353 224 210 369 317 233 236 280 485 459 573 128 354 254 182 320 326


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.5 14.2 11.7 10.6 10.3 8.9 7.7 8.7 7.4 7.4 7.3 8.0 11.2 12.2 12.1 16.1 15.7 15.9 12.1 12.4 13.7
Std Deviation (1e-4) 1.5 2.3 1.8 1.7 1.7 2.1 1.7 1.7 1.4 1.6 1.5 2.0 1.9 1.8 2.0 4.8 5.0 4.9 2.1 2.2 2.5
Intensity Change (Log2) -0.14 -0.18 -0.38 -0.59 -0.43 -0.66 -0.66 -0.68 -0.54 -0.06 0.07 0.06 0.47 0.43 0.45 0.05 0.09 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 3.1 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.4 -0.1 0.6 -0.3 1.0 -0.5 0 0.1 1.3 -2.1 -0.8 0.6 2.5 1.3 2.4
Cytoplasm -1.5 -4.4 -5.0 -7.7 -7.9 -7.1 -9.1 -9.5 -8.4 -0.1 1.2 -0.6 -5.2 -4.8 -4.4
Endoplasmic Reticulum 1.0 2.9 1.5 1.5 2.6 1.7 0 2.4 2.4 0 0 0.4 0.7 0.8 1.0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.3 8.6 6.8 8.1 10.2 10.1 7.4 0 0 0 0 0 3.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.5 3.6 0 3.0 2.4 2.6 0 0 0 5.4 5.0 3.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin
Bud
Bud Neck
Bud Periphery
Bud Site
Cell Periphery
Cytoplasm
Cytoplasmic Foci
Eisosomes
Endoplasmic Reticulum
Endosome
Golgi
Lipid Particles
Mitochondria
None
Nuclear Periphery
Nucleolus
Nucleus
Peroxisomes
Punctate Nuclear
Vacuole
Vacuole Periphery

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.3114 16.6711 10.9962 28.2501 18.8062 16.5678 28.796 30.3381 30.8477 34.5916
Translational Efficiency 2.3933 2.7257 4.373 1.327 2.7338 4.2783 1.8102 1.672 1.8441 1.8029

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1218 2100 1765 236 2136 2257 1845 1076 3354 4357 3610 1312

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 858.63 1276.05 1332.65 1478.01 1086.70 1204.37 1311.59 1482.03 1003.88 1238.92 1321.89 1481.31
Standard Deviation 146.00 194.78 178.31 192.01 160.45 186.84 174.33 236.24 190.17 194.04 176.60 228.92
Intensity Change Log 2 0.571576 0.634189 0.783548 0.148325 0.271363 0.447621 0.350570 0.442838 0.605602

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000332 0.000064 0.000087 0.000302 0.000147 0.000093 0.000118 0.000975 0.000214 0.000079 0.000103 0.000854
Bud Neck 0.001827 0.000213 0.000568 0.002089 0.000550 0.000210 0.000564 0.000992 0.001013 0.000211 0.000566 0.001189
Bud Site 0.049590 0.009461 0.006968 0.020736 0.013660 0.007688 0.006293 0.019324 0.026708 0.008542 0.006623 0.019578
Cell Periphery 0.000383 0.000118 0.000068 0.000307 0.000233 0.000081 0.000097 0.000680 0.000287 0.000099 0.000083 0.000613
Cytoplasm 0.562436* 0.878239* 0.864113* 0.684853* 0.767192* 0.876621* 0.859025* 0.904502* 0.692835* 0.877401* 0.861512* 0.864992*
Cytoplasmic Foci 0.259285* 0.066494 0.032100 0.062961 0.148315 0.082387 0.041876 0.026450 0.188614* 0.074727 0.037096 0.033018
Eisosomes 0.000027 0.000001 0.000002 0.000007 0.000008 0.000002 0.000003 0.000005 0.000015 0.000002 0.000002 0.000006
Endoplasmic Reticulum 0.007324 0.001468 0.000984 0.002017 0.007688 0.002078 0.001749 0.000284 0.007556 0.001784 0.001375 0.000595
Endosome 0.014681 0.002138 0.003502 0.010864 0.006405 0.002255 0.003336 0.002264 0.009411 0.002199 0.003417 0.003811
Golgi 0.021486 0.010271 0.002535 0.002684 0.014497 0.009574 0.002260 0.006378 0.017035 0.009910 0.002395 0.005714
Lipid Particles 0.001140 0.000368 0.000687 0.003791 0.001163 0.000434 0.000877 0.004474 0.001154 0.000402 0.000784 0.004352
Mitochondria 0.010654 0.000721 0.000230 0.000016 0.001001 0.000261 0.000041 0.000217 0.004507 0.000483 0.000133 0.000180
Mitotic Spindle 0.001413 0.001561 0.001765 0.000323 0.001168 0.000970 0.000977 0.002181 0.001257 0.001255 0.001363 0.001847
None 0.001156 0.000821 0.000342 0.000105 0.000525 0.001210 0.000761 0.000506 0.000754 0.001023 0.000556 0.000434
Nuclear Periphery 0.000055 0.000022 0.000040 0.000074 0.000033 0.000011 0.000050 0.000024 0.000041 0.000016 0.000045 0.000033
Nuclear Periphery Foci 0.000398 0.000460 0.000874 0.002044 0.000335 0.000316 0.001421 0.001725 0.000358 0.000386 0.001154 0.001783
Nucleolus 0.000313 0.000030 0.000123 0.000092 0.000051 0.000031 0.000072 0.000072 0.000146 0.000031 0.000097 0.000075
Nucleus 0.004399 0.002340 0.007893 0.006636 0.002725 0.002731 0.009960 0.004068 0.003333 0.002542 0.008949 0.004529
Peroxisomes 0.000815 0.000118 0.000284 0.000798 0.000296 0.000145 0.000169 0.000638 0.000484 0.000132 0.000225 0.000667
Vacuole 0.026382 0.011097 0.071466 0.194679 0.019758 0.007672 0.068367 0.022775 0.022163 0.009323 0.069882 0.053696
Vacuole Periphery 0.035905 0.013993 0.005368 0.004622 0.014252 0.005230 0.001985 0.001467 0.022115 0.009453 0.003639 0.002035

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 5.61 4.99 0.90 -5.98 -5.08 3.24 2.11 -2.60 -2.77 -2.68 6.81 5.53 -2.41 -2.95 -2.85
Bud Neck 9.01 6.52 -1.13 -8.41 -6.53 7.22 -0.53 -4.81 -8.06 -4.56 10.98 5.21 -2.47 -10.62 -6.63
Bud Site 18.37* 19.35* 7.75 -3.10 -3.82 7.98 9.10 -3.46 -7.21 -7.89 18.35 19.83 4.10 -7.40 -8.56
Cell Periphery 8.93 10.88 1.39 -2.80 -3.63 9.23 7.80 -6.26 -8.59 -8.33 12.37 13.46 -5.36 -8.75 -9.02
Cytoplasm -35.75* -33.47 -5.96 10.15 9.29 -17.49 -13.12 -19.10 -3.88 -6.44 -34.41 -29.78 -23.03 2.61 -0.21
Cytoplasmic Foci 30.75* 38.28 25.00 1.08 -4.78 14.37 26.41 30.26 17.15 6.38 30.26 43.88 42.30 16.36 2.20
Eisosomes 9.42 9.27 7.23 -7.94 -7.29 15.27 11.72 2.41 -4.17 -2.88 12.81 12.03 7.15 -5.65 -4.59
Endoplasmic Reticulum 7.39 8.19 4.31 -0.33 -0.80 5.74 6.22 8.33 4.25 3.83 8.58 9.40 10.64 3.96 2.86
Endosome 15.38 12.90 3.03 -5.91 -4.76 10.70 7.46 7.79 0.06 2.39 18.56 14.28 10.31 -3.28 -0.41
Golgi 11.94 22.19 21.11 12.37 0.20 8.68 22.68 7.06 3.01 -3.43 14.03 31.65 11.66 4.70 -3.33
Lipid Particles 6.38 3.54 -2.39 -3.16 -2.84 9.34 2.93 -3.94 -4.78 -4.25 11.37 4.93 -4.41 -5.44 -4.91
Mitochondria 5.30 5.59 5.71 3.34 2.36 2.89 3.77 2.98 0.66 -2.10 5.69 6.23 6.16 2.55 -0.48
Mitotic Spindle -0.24 -0.52 3.62 3.62 2.11 0.53 0.34 -1.05 -1.28 -1.17 0.07 -0.27 -0.76 -0.79 -0.55
None 1.13 2.90 3.71 6.63 6.16 -4.08 -2.01 0.29 4.38 2.44 -1.84 1.70 2.68 5.66 1.82
Nuclear Periphery 3.22 1.14 -0.80 -2.16 -1.36 3.33 -1.80 0.44 -0.97 1.49 4.35 -0.67 0.43 -1.41 0.79
Nuclear Periphery Foci -0.36 -1.50 -4.17 -4.17 -2.36 0.43 -9.38 -2.78 -2.81 -0.72 -0.40 -4.81 -3.37 -3.34 -1.47
Nucleolus 2.91 1.89 2.25 -6.55 1.35 7.90 -5.23 -2.47 -4.40 -0.27 3.24 1.29 1.86 -5.71 1.51
Nucleus 2.11 -3.53 -2.35 -4.70 -0.04 -0.06 -17.33 -6.80 -6.83 12.16 2.14 -12.91 -3.76 -8.50 10.35
Peroxisomes 5.47 3.87 0.61 -6.88 -4.27 10.31 7.50 -3.83 -5.54 -5.24 7.32 4.68 -1.78 -7.06 -5.38
Vacuole 6.65 -12.64 -11.01 -12.07 -8.02 9.66 -13.94 -3.06 -9.84 11.36 11.01 -19.61 -9.26 -13.00 3.18
Vacuole Periphery 8.55 13.39 12.97 6.13 1.05 8.13 12.06 12.52 6.54 1.42 10.26 17.20 18.68 10.40 4.20
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Componentsactinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Nitrogen catabolite repression transcriptional regulator; inhibits GLN3 transcription in good nitrogen source; role in sequestering Gln3p and Gat1p to the cytoplasm; has glutathione peroxidase activity and can mutate to acquire GST activity; self-assembly under limited nitrogen conditions creates [URE3] prion and releases catabolite repression
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Ure2

Ure2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ure2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available