Standard name
Human Ortholog
Description Nitrogen catabolite repression transcriptional regulator; inhibits GLN3 transcription in good nitrogen source; role in sequestering Gln3p and Gat1p to the cytoplasm; has glutathione peroxidase activity and can mutate to acquire GST activity; self-assembly under limited nitrogen conditions creates [URE3] prion and releases catabolite repression
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0.05 0 0.06 0 0.06 0 0.05 0.05 0.07 0 0 0.06 0.1 0.07 0.1 0 0 0 0 0 0
Cytoplasm 0.98 0.96 0.97 0.94 0.86 0.84 0.72 0.72 0.74 0.63 0.64 0.69 0.97 0.99 0.96 0.84 0.86 0.87 0.94 0.93 0.91 0.85 0.89 0.89
Endoplasmic Reticulum 0 0 0 0 0.07 0.05 0 0.07 0.05 0 0.06 0.06 0 0 0 0 0 0 0 0 0 0.06 0.05 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.11 0.27 0.17 0.24 0.36 0.34 0.2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.07 0 0.06 0 0.05 0 0 0 0.13 0.11 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 3 0 5 6 6 4 4 11 0 0 1 1 0 4 0 0 2 0 0 1
Bud 0 0 0 0 0 0 0 5 1 3 6 4 0 0 0 0 0 1 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 10 11 10 14 11 10 20 7 8 15 20 3 7 15 43 29 49 0 0 1 2 0 1
Cytoplasm 104 329 237 180 279 162 179 224 146 111 203 193 222 223 255 364 354 442 120 328 230 155 285 291
Endoplasmic Reticulum 0 9 5 7 24 9 11 21 10 5 20 18 4 3 7 13 13 17 2 8 9 10 16 11
Endosome 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0
Golgi 0 0 0 0 2 1 0 1 0 0 0 0 0 2 1 2 2 1 0 1 0 1 1 1
Mitochondria 2 3 0 5 10 21 66 53 47 64 107 56 0 0 0 4 9 18 0 2 2 4 1 5
Nucleus 0 0 2 1 2 0 4 0 3 3 1 1 0 1 1 2 5 5 0 0 0 2 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 2 2 0 1 0 0 0 0 0 1 0 1 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 2 2 4 8 7 11 22 2 10 13 13 4 0 0 56 47 35 0 5 5 3 8 6
Unique Cell Count 106 343 244 191 323 194 249 312 197 176 319 281 229 226 265 434 414 508 128 354 254 182 320 326
Labelled Cell Count 108 353 257 208 342 211 286 353 224 210 369 317 233 236 280 485 459 573 128 354 254 182 320 326


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.5 14.2 11.7 10.6 10.3 8.9 7.7 8.7 7.4 7.4 7.3 8.0 11.2 12.2 12.1 16.1 15.7 15.9 12.1 12.4 13.7
Std Deviation (1e-4) 1.5 2.3 1.8 1.7 1.7 2.1 1.7 1.7 1.4 1.6 1.5 2.0 1.9 1.8 2.0 4.8 5.0 4.9 2.1 2.2 2.5
Intensity Change (Log2) – – – -0.14 -0.18 -0.38 -0.59 -0.43 -0.66 -0.66 -0.68 -0.54 -0.06 0.07 0.06 0.47 0.43 0.45 0.05 0.09 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 3.1 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.4 -0.1 0.6 -0.3 1.0 -0.5 0 0.1 1.3 -2.1 -0.8 0.6 2.5 1.3 2.4
Cytoplasm -1.5 -4.4 -5.0 -7.7 -7.9 -7.1 -9.1 -9.5 -8.4 -0.1 1.2 -0.6 -5.2 -4.8 -4.4
Endoplasmic Reticulum 1.0 2.9 1.5 1.5 2.6 1.7 0 2.4 2.4 0 0 0.4 0.7 0.8 1.0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.3 8.6 6.8 8.1 10.2 10.1 7.4 0 0 0 0 0 3.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.5 3.6 0 3.0 2.4 2.6 0 0 0 5.4 5.0 3.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin – – – – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – – – – –
Bud Periphery – – – – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – – – – –
Cytoplasmic Foci – – – – – – – – – – – – – – – – – –
Eisosomes – – – – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – – – – –
Lipid Particles – – – – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – – – – –
None – – – – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – – – – –
Punctate Nuclear – – – – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – – – – –
Vacuole Periphery – – – – – – – – – – – – – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.3114 16.6711 10.9962 28.2501 18.8062 16.5678 28.796 30.3381 30.8477 34.5916
Translational Efficiency 2.3933 2.7257 4.373 1.327 2.7338 4.2783 1.8102 1.672 1.8441 1.8029

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization –
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1218 2100 1765 236 2136 2257 1845 1076 3354 4357 3610 1312

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 858.63 1276.05 1332.65 1478.01 1086.70 1204.37 1311.59 1482.03 1003.88 1238.92 1321.89 1481.31
Standard Deviation 146.00 194.78 178.31 192.01 160.45 186.84 174.33 236.24 190.17 194.04 176.60 228.92
Intensity Change Log 2 — 0.571576 0.634189 0.783548 — 0.148325 0.271363 0.447621 — 0.350570 0.442838 0.605602

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000332 0.000064 0.000087 0.000302 0.000147 0.000093 0.000118 0.000975 0.000214 0.000079 0.000103 0.000854
Bud Neck 0.001827 0.000213 0.000568 0.002089 0.000550 0.000210 0.000564 0.000992 0.001013 0.000211 0.000566 0.001189
Bud Site 0.049590 0.009461 0.006968 0.020736 0.013660 0.007688 0.006293 0.019324 0.026708 0.008542 0.006623 0.019578
Cell Periphery 0.000383 0.000118 0.000068 0.000307 0.000233 0.000081 0.000097 0.000680 0.000287 0.000099 0.000083 0.000613
Cytoplasm 0.562436* 0.878239* 0.864113* 0.684853* 0.767192* 0.876621* 0.859025* 0.904502* 0.692835* 0.877401* 0.861512* 0.864992*
Cytoplasmic Foci 0.259285* 0.066494 0.032100 0.062961 0.148315 0.082387 0.041876 0.026450 0.188614* 0.074727 0.037096 0.033018
Eisosomes 0.000027 0.000001 0.000002 0.000007 0.000008 0.000002 0.000003 0.000005 0.000015 0.000002 0.000002 0.000006
Endoplasmic Reticulum 0.007324 0.001468 0.000984 0.002017 0.007688 0.002078 0.001749 0.000284 0.007556 0.001784 0.001375 0.000595
Endosome 0.014681 0.002138 0.003502 0.010864 0.006405 0.002255 0.003336 0.002264 0.009411 0.002199 0.003417 0.003811
Golgi 0.021486 0.010271 0.002535 0.002684 0.014497 0.009574 0.002260 0.006378 0.017035 0.009910 0.002395 0.005714
Lipid Particles 0.001140 0.000368 0.000687 0.003791 0.001163 0.000434 0.000877 0.004474 0.001154 0.000402 0.000784 0.004352
Mitochondria 0.010654 0.000721 0.000230 0.000016 0.001001 0.000261 0.000041 0.000217 0.004507 0.000483 0.000133 0.000180
Mitotic Spindle 0.001413 0.001561 0.001765 0.000323 0.001168 0.000970 0.000977 0.002181 0.001257 0.001255 0.001363 0.001847
None 0.001156 0.000821 0.000342 0.000105 0.000525 0.001210 0.000761 0.000506 0.000754 0.001023 0.000556 0.000434
Nuclear Periphery 0.000055 0.000022 0.000040 0.000074 0.000033 0.000011 0.000050 0.000024 0.000041 0.000016 0.000045 0.000033
Nuclear Periphery Foci 0.000398 0.000460 0.000874 0.002044 0.000335 0.000316 0.001421 0.001725 0.000358 0.000386 0.001154 0.001783
Nucleolus 0.000313 0.000030 0.000123 0.000092 0.000051 0.000031 0.000072 0.000072 0.000146 0.000031 0.000097 0.000075
Nucleus 0.004399 0.002340 0.007893 0.006636 0.002725 0.002731 0.009960 0.004068 0.003333 0.002542 0.008949 0.004529
Peroxisomes 0.000815 0.000118 0.000284 0.000798 0.000296 0.000145 0.000169 0.000638 0.000484 0.000132 0.000225 0.000667
Vacuole 0.026382 0.011097 0.071466 0.194679 0.019758 0.007672 0.068367 0.022775 0.022163 0.009323 0.069882 0.053696
Vacuole Periphery 0.035905 0.013993 0.005368 0.004622 0.014252 0.005230 0.001985 0.001467 0.022115 0.009453 0.003639 0.002035

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 5.61 4.99 0.90 -5.98 -5.08 3.24 2.11 -2.60 -2.77 -2.68 6.81 5.53 -2.41 -2.95 -2.85
Bud Neck 9.01 6.52 -1.13 -8.41 -6.53 7.22 -0.53 -4.81 -8.06 -4.56 10.98 5.21 -2.47 -10.62 -6.63
Bud Site 18.37* 19.35* 7.75 -3.10 -3.82 7.98 9.10 -3.46 -7.21 -7.89 18.35 19.83 4.10 -7.40 -8.56
Cell Periphery 8.93 10.88 1.39 -2.80 -3.63 9.23 7.80 -6.26 -8.59 -8.33 12.37 13.46 -5.36 -8.75 -9.02
Cytoplasm -35.75* -33.47 -5.96 10.15 9.29 -17.49 -13.12 -19.10 -3.88 -6.44 -34.41 -29.78 -23.03 2.61 -0.21
Cytoplasmic Foci 30.75* 38.28 25.00 1.08 -4.78 14.37 26.41 30.26 17.15 6.38 30.26 43.88 42.30 16.36 2.20
Eisosomes 9.42 9.27 7.23 -7.94 -7.29 15.27 11.72 2.41 -4.17 -2.88 12.81 12.03 7.15 -5.65 -4.59
Endoplasmic Reticulum 7.39 8.19 4.31 -0.33 -0.80 5.74 6.22 8.33 4.25 3.83 8.58 9.40 10.64 3.96 2.86
Endosome 15.38 12.90 3.03 -5.91 -4.76 10.70 7.46 7.79 0.06 2.39 18.56 14.28 10.31 -3.28 -0.41
Golgi 11.94 22.19 21.11 12.37 0.20 8.68 22.68 7.06 3.01 -3.43 14.03 31.65 11.66 4.70 -3.33
Lipid Particles 6.38 3.54 -2.39 -3.16 -2.84 9.34 2.93 -3.94 -4.78 -4.25 11.37 4.93 -4.41 -5.44 -4.91
Mitochondria 5.30 5.59 5.71 3.34 2.36 2.89 3.77 2.98 0.66 -2.10 5.69 6.23 6.16 2.55 -0.48
Mitotic Spindle -0.24 -0.52 3.62 3.62 2.11 0.53 0.34 -1.05 -1.28 -1.17 0.07 -0.27 -0.76 -0.79 -0.55
None 1.13 2.90 3.71 6.63 6.16 -4.08 -2.01 0.29 4.38 2.44 -1.84 1.70 2.68 5.66 1.82
Nuclear Periphery 3.22 1.14 -0.80 -2.16 -1.36 3.33 -1.80 0.44 -0.97 1.49 4.35 -0.67 0.43 -1.41 0.79
Nuclear Periphery Foci -0.36 -1.50 -4.17 -4.17 -2.36 0.43 -9.38 -2.78 -2.81 -0.72 -0.40 -4.81 -3.37 -3.34 -1.47
Nucleolus 2.91 1.89 2.25 -6.55 1.35 7.90 -5.23 -2.47 -4.40 -0.27 3.24 1.29 1.86 -5.71 1.51
Nucleus 2.11 -3.53 -2.35 -4.70 -0.04 -0.06 -17.33 -6.80 -6.83 12.16 2.14 -12.91 -3.76 -8.50 10.35
Peroxisomes 5.47 3.87 0.61 -6.88 -4.27 10.31 7.50 -3.83 -5.54 -5.24 7.32 4.68 -1.78 -7.06 -5.38
Vacuole 6.65 -12.64 -11.01 -12.07 -8.02 9.66 -13.94 -3.06 -9.84 11.36 11.01 -19.61 -9.26 -13.00 3.18
Vacuole Periphery 8.55 13.39 12.97 6.13 1.05 8.13 12.06 12.52 6.54 1.42 10.26 17.20 18.68 10.40 4.20
Standard name
Human Ortholog
Description Nitrogen catabolite repression transcriptional regulator; inhibits GLN3 transcription in good nitrogen source; role in sequestering Gln3p and Gat1p to the cytoplasm; has glutathione peroxidase activity and can mutate to acquire GST activity; self-assembly under limited nitrogen conditions creates [URE3] prion and releases catabolite repression
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Ure2

Ure2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ure2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available