Standard name
Human Ortholog
Description Elongin A; F-box protein that forms a heterodimer with Elc1p and is required for ubiquitin-dependent degradation of the RNA Polymerase II subunit Rpo21p; subunit of the Elongin-Cullin-Socs (ECS) ligase complex
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.51 – – 0.5 0.71 0.35 0.36 0.39 0.15 0.26 0.18 0.16 0.74 0.71 0.67 0.54 0.48 0.45 0.44 0.42 0.4 0.71 0.69 0.62
Endoplasmic Reticulum 0 – – 0 0 0.06 0 0 0 0 0 0 0 0 0 0.06 0.08 0 0 0 0 0 0 0
Endosome 0 – – 0 0 0 0 0 0 0 0 0 0 0.05 0.1 0.06 0 0.11 0 0 0 0 0 0
Golgi 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.62 – – 0.53 0.28 0.59 0.66 0.69 0.84 0.72 0.87 0.88 0 0 0 0 0.1 0.07 0.12 0 0.16 0 0 0
Nucleus 0.05 – – 0 0.07 0.12 0 0.08 0.09 0 0.1 0.05 0.11 0.11 0.09 0.15 0.11 0.17 0.09 0.19 0.11 0.08 0.05 0.05
Nuclear Periphery 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0.06
Vac/Vac Membrane 0.15 – – 0.12 0 0.12 0.09 0.11 0.1 0.09 0.16 0.11 0.14 0.19 0.22 0.31 0.38 0.42 0.28 0.25 0.17 0.09 0.1 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 4
Bud 0 – – 0 1 0 1 0 1 1 0 0 0 0 0 0 0 0 1 0 0 3 7 18
Bud Neck 0 – – 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 6 10
Bud Site 0 – – 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 2
Cytoplasm 65 – – 17 61 6 31 34 13 14 14 14 220 340 359 36 60 38 66 32 17 120 342 370
Endoplasmic Reticulum 0 – – 0 0 1 1 1 2 1 1 0 0 1 0 4 10 3 0 0 0 0 0 0
Endosome 0 – – 0 0 0 0 0 0 0 0 0 10 23 53 4 5 9 1 0 0 1 5 4
Golgi 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 2
Mitochondria 79 – – 18 24 10 57 60 74 39 67 75 1 1 7 3 12 6 17 1 7 5 19 19
Nucleus 6 – – 1 6 2 2 7 8 2 8 4 32 54 46 10 14 14 14 14 4 14 26 30
Nuclear Periphery 1 – – 0 0 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 – – 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – – 0 1 0 1 0 1 0 0 0 3 1 0 0 1 1 1 4 1 3 18 35
Vac/Vac Membrane 19 – – 4 3 2 8 10 9 5 12 9 42 93 116 21 47 35 42 19 7 15 51 76
Unique Cell Count 128 34 86 17 86 87 88 54 77 85 297 478 534 67 125 84 152 78 44 170 499 600
Labelled Cell Count 170 40 96 21 103 112 109 62 102 105 313 514 581 78 149 107 152 78 44 170 499 600


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 – – 3.6 3.7 3.3 3.3 3.3 3.2 3.2 3.0 3.1 5.4 5.4 5.4 5.4 5.5 5.7 5.4 5.3 5.7
Std Deviation (1e-4) 0.4 – – 1.8 0.5 0.6 0.7 0.5 0.5 1.1 0.6 0.6 1.5 1.4 1.5 0.9 1.5 1.7 1.7 1.4 2.0
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.8099 -0.6579 -1.0003 -1.3118 -1.1602 -0.8958 -1.6875 -1.3819 -1.3743 -1.5172 -1.7753 -1.4004 -2.3251 -1.9207 -1.919 -1.9818 -1.2138 -1.9054
Actin 0.0302 0.0006 0.0047 0.0004 0.002 0.0004 0.0298 0.0009 0.0043 0.0103 0.0002 0.0062 0.0083 0.0001 0.0168 0.0285 0.0566 0.0034
Bud 0.0021 0.0002 0.003 0.0208 0.0008 0.0007 0.0004 0.0011 0.0009 0.0016 0 0.0004 0.0006 0 0.0007 0.0016 0.0026 0.0003
Bud Neck 0.0024 0.0001 0.0004 0.0002 0.0006 0.002 0.0055 0.0008 0.0009 0.0011 0.0004 0.0026 0.0017 0.0002 0.0014 0.003 0.002 0.0015
Bud Periphery 0.0013 0.0002 0.0009 0.0117 0.0002 0.0002 0.0004 0.0026 0.0011 0.0025 0 0.0012 0.0004 0 0.0007 0.0041 0.0081 0.0005
Bud Site 0.0025 0.0008 0.0051 0.0009 0.0003 0.0001 0.0027 0.0054 0.0018 0.0031 0.0001 0.0002 0.001 0.0007 0.006 0.0026 0.0058 0.0014
Cell Periphery 0.0003 0 0.0001 0.0004 0 0 0.0001 0.0002 0.0001 0.0002 0 0 0.0001 0 0.0003 0.0002 0.0004 0.0001
Cytoplasm 0.1143 0.1525 0.1396 0.1104 0.16 0.2199 0.2294 0.2535 0.265 0.2531 0.3898 0.2915 0.2437 0.3475 0.2494 0.3209 0.1711 0.3259
Cytoplasmic Foci 0.0247 0.0048 0.0265 0.0135 0.0152 0.0299 0.0174 0.0086 0.0118 0.041 0.0052 0.0117 0.0224 0.0054 0.0178 0.0137 0.0444 0.0122
Eisosomes 0.0005 0.0001 0.0002 0.0001 0.0001 0 0.0004 0.0001 0.0002 0.0001 0 0.0001 0.0001 0 0.0003 0.0001 0.0008 0.0001
Endoplasmic Reticulum 0.0064 0.0028 0.0023 0.0038 0.0016 0.0032 0.0041 0.0034 0.0053 0.0077 0.0038 0.0035 0.0062 0.0044 0.004 0.0116 0.0042 0.0051
Endosome 0.0219 0.0024 0.0055 0.0125 0.0032 0.006 0.0114 0.0027 0.0066 0.0088 0.0055 0.0056 0.0149 0.0044 0.0071 0.0079 0.0248 0.0078
Golgi 0.0062 0.0002 0.0006 0.0003 0.0003 0.0002 0.0043 0.0002 0.0011 0.0037 0.0001 0.0006 0.0026 0.0001 0.0025 0.004 0.0114 0.0019
Lipid Particles 0.0104 0.0004 0.0048 0.0015 0.0029 0.001 0.0051 0.0005 0.0012 0.0326 0.0002 0.0009 0.0064 0.0003 0.0047 0.0045 0.0268 0.0026
Mitochondria 0.004 0.0006 0.0009 0.0021 0.0005 0.0009 0.0013 0.0016 0.0061 0.011 0.0006 0.0007 0.0105 0.0003 0.0021 0.0026 0.0765 0.0037
None 0.6354 0.7213 0.6595 0.5798 0.6888 0.4668 0.5671 0.5438 0.5138 0.3831 0.417 0.3741 0.4864 0.4957 0.5016 0.4136 0.283 0.387
Nuclear Periphery 0.0202 0.0062 0.0138 0.0134 0.0087 0.0096 0.0142 0.0089 0.0181 0.0271 0.0076 0.0128 0.0245 0.0111 0.0191 0.0182 0.0506 0.01
Nucleolus 0.0056 0.0009 0.0023 0.0024 0.0033 0.0039 0.0019 0.0018 0.0013 0.0011 0.0005 0.0019 0.0033 0.0008 0.0012 0.0014 0.0022 0.0016
Nucleus 0.0687 0.0956 0.0988 0.1793 0.0555 0.1766 0.0767 0.1409 0.1351 0.163 0.1399 0.2519 0.1308 0.1141 0.1329 0.118 0.1682 0.2076
Peroxisomes 0.0096 0.001 0.0026 0.0006 0.0012 0.0004 0.0032 0.0008 0.0011 0.013 0.0001 0.0002 0.004 0.0001 0.0067 0.0215 0.0123 0.0008
Punctate Nuclear 0.025 0.0078 0.0271 0.0433 0.0541 0.0767 0.022 0.0204 0.0211 0.0297 0.027 0.0314 0.0275 0.0126 0.0225 0.0174 0.0424 0.0242
Vacuole 0.0071 0.001 0.0009 0.0016 0.0007 0.0011 0.0018 0.0016 0.0023 0.0046 0.0016 0.002 0.0034 0.002 0.0017 0.0034 0.0028 0.0018
Vacuole Periphery 0.0013 0.0002 0.0004 0.0008 0.0002 0.0003 0.0006 0.0004 0.0007 0.0016 0.0004 0.0005 0.0011 0.0003 0.0006 0.001 0.0031 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.7432 1.7305 7.7046 7.087 9.4119 11.6084 10.9356 4.1332 7.7054 9.1081
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
727 210 1051 254 1618 225 261 414 2345 435 1312 668

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 661.16 768.40 748.92 832.14 666.30 719.29 879.81 855.70 664.71 743.00 774.96 846.74
Standard Deviation 72.64 93.96 95.71 93.33 72.11 84.33 104.86 111.68 72.31 92.43 110.70 105.70
Intensity Change Log 2 — 0.216858 0.179812 0.331827 — 0.110402 0.401020 0.360933 — 0.164406 0.295079 0.346510

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000119 0.000637 0.000415 0.000496 0.000057 0.000506 0.000473 0.004020 0.000076 0.000569 0.000427 0.002680
Bud Neck 0.006178 0.020527 0.007727 0.005868 0.004344 0.014014 0.008099 0.008177 0.004912 0.017158 0.007801 0.007299
Bud Site 0.001050 0.004383 0.010681 0.003288 0.001994 0.010386 0.002061 0.026382 0.001701 0.007488 0.008966 0.017601
Cell Periphery 0.000138 0.000180 0.000245 0.000149 0.000143 0.000197 0.000125 0.000518 0.000141 0.000189 0.000221 0.000378
Cytoplasm 0.373512* 0.265837* 0.117413* 0.139818* 0.417218* 0.232268* 0.070818 0.234172* 0.403668* 0.248474* 0.108144* 0.198295*
Cytoplasmic Foci 0.160900 0.191636* 0.002676 0.008522 0.098035 0.150082 0.002019 0.005976 0.117525 0.170143 0.002545 0.006944
Eisosomes 0.000107 0.000137 0.000034 0.000059 0.000078 0.000104 0.000060 0.000060 0.000087 0.000120 0.000039 0.000060
Endoplasmic Reticulum 0.000740 0.002736 0.005054 0.011715 0.000672 0.001207 0.008187 0.005178 0.000693 0.001945 0.005678 0.007664
Endosome 0.004297 0.010711 0.001315 0.006386 0.002466 0.009747 0.001591 0.009162 0.003033 0.010212 0.001370 0.008106
Golgi 0.000926 0.001881 0.000131 0.000667 0.000477 0.003838 0.000058 0.003952 0.000616 0.002893 0.000116 0.002703
Lipid Particles 0.009188 0.009702 0.001013 0.001904 0.005403 0.006772 0.001429 0.001260 0.006576 0.008187 0.001096 0.001504
Mitochondria 0.004354 0.006510 0.001767 0.000857 0.002637 0.004809 0.000600 0.004419 0.003169 0.005630 0.001535 0.003065
Mitotic Spindle 0.001368 0.002832 0.002613 0.002606 0.003555 0.013863 0.000438 0.005990 0.002877 0.008538 0.002180 0.004704
None 0.004470 0.007836 0.006494 0.007471 0.005653 0.005094 0.006902 0.012701 0.005286 0.006418 0.006575 0.010712
Nuclear Periphery 0.000874 0.000937 0.001974 0.002947 0.000839 0.000970 0.002073 0.002054 0.000850 0.000954 0.001994 0.002394
Nuclear Periphery Foci 0.000450 0.000946 0.000493 0.002129 0.000347 0.000721 0.000744 0.000909 0.000379 0.000829 0.000543 0.001373
Nucleolus 0.001784 0.002663 0.001602 0.000968 0.001387 0.003178 0.001562 0.000682 0.001510 0.002929 0.001594 0.000791
Nucleus 0.405615* 0.404062* 0.817996* 0.742530* 0.436831* 0.502025* 0.860952* 0.594773* 0.427153* 0.454733* 0.826541* 0.650956*
Peroxisomes 0.007516 0.022294 0.000374 0.001545 0.002422 0.008140 0.000214 0.000620 0.004001 0.014973 0.000342 0.000972
Vacuole 0.015966 0.042435 0.018691 0.059436 0.014759 0.030734 0.031415 0.074315 0.015133 0.036383 0.021222 0.068657
Vacuole Periphery 0.000448 0.001120 0.001292 0.000638 0.000686 0.001346 0.000182 0.004680 0.000612 0.001237 0.001071 0.003143

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.92 -6.65 -8.09 -0.60 -3.75 -1.99 -8.24 -2.70 -1.88 -1.42 -2.82 -11.60 -3.39 -1.93 -2.15
Bud Neck -2.63 -1.47 -2.24 2.03 -0.75 -2.76 -1.49 -5.25 0.81 -1.54 -3.87 -3.15 -5.82 2.05 -1.84
Bud Site -2.93 -4.75 -6.05 -0.08 2.78 -1.82 -1.45 -3.46 -0.26 -3.11 -2.38 -4.45 -4.13 -0.50 -0.15
Cell Periphery -2.13 -3.30 -1.50 0.94 2.38 -1.33 -0.43 -1.36 -0.41 -1.22 -2.09 -3.28 -1.55 -0.27 0.33
Cytoplasm 5.98 24.22 19.49 8.19 0.43 11.67 40.53 23.60 5.09 -7.25 13.09 43.20 30.87 9.03 -3.17
Cytoplasmic Foci -1.85 23.09 22.29 12.90 -1.87 -4.00 28.80 27.99 11.82 -2.63 -5.16 36.08 34.94 17.45 -2.70
Eisosomes -2.45 10.33 2.20 3.92 -6.99 -2.84 -1.16 -1.23 1.97 0.30 -4.39 9.52 0.17 4.32 -8.03
Endoplasmic Reticulum -2.68 -14.80 -11.19 -8.13 -6.74 -2.99 -11.52 -11.78 -11.04 1.16 -3.32 -18.59 -16.17 -12.78 -6.36
Endosome -3.48 4.42 0.37 3.20 -1.84 -3.78 1.03 -1.53 2.54 -1.86 -5.45 5.18 -1.12 4.02 -2.95
Golgi -1.07 3.40 2.19 1.96 -0.93 -1.30 5.03 -0.81 1.03 -1.39 -1.61 5.43 -0.29 1.44 -1.48
Lipid Particles -0.24 9.10 7.70 4.74 -3.90 -1.49 7.47 8.88 5.39 0.67 -1.69 13.45 11.60 6.98 -3.59
Mitochondria -0.81 1.75 2.26 2.76 2.27 -1.48 2.73 0.55 1.88 -2.38 -1.80 2.29 1.91 3.20 -0.37
Mitotic Spindle -1.19 -1.46 -1.74 -1.06 -1.29 -1.60 -0.10 -2.77 0.29 -2.19 -1.71 -0.15 -3.18 -0.32 -3.14
None -0.78 -0.93 -1.27 0.43 -0.06 0.38 1.16 -1.43 -1.30 -2.34 -0.42 -0.11 -1.70 -0.33 -1.18
Nuclear Periphery -0.85 -9.07 -10.91 -10.34 -6.48 -1.01 -9.96 -11.49 -10.43 -3.67 -1.35 -12.31 -15.53 -14.43 -8.24
Nuclear Periphery Foci -2.58 -1.17 -4.44 -2.81 -4.16 -1.87 -4.24 -5.20 -3.30 -0.88 -3.31 -4.34 -6.87 -4.23 -5.09
Nucleolus -0.79 1.11 1.98 3.10 0.92 -1.78 0.88 2.47 2.21 0.89 -2.17 0.73 3.08 3.18 1.39
Nucleus 0.10 -32.99 -16.09 -11.85 6.01 -2.97 -26.75 -12.51 -5.37 9.55 -1.72 -45.56 -19.31 -11.49 11.88
Peroxisomes -2.91 5.66 5.23 4.47 -0.96 -2.28 4.51 4.22 3.19 -1.30 -4.00 7.03 6.59 5.44 -0.98
Vacuole -5.32 -4.20 -8.65 -5.40 -7.68 -4.37 -6.63 -12.07 -9.92 -5.07 -6.93 -8.11 -14.85 -10.89 -11.71
Vacuole Periphery -2.44 -1.01 0.70 2.59 1.17 -1.05 1.78 -0.64 0.03 -1.22 -1.62 -0.52 -0.53 0.41 -0.08
Standard name
Human Ortholog
Description Elongin A; F-box protein that forms a heterodimer with Elc1p and is required for ubiquitin-dependent degradation of the RNA Polymerase II subunit Rpo21p; subunit of the Elongin-Cullin-Socs (ECS) ligase complex
Localization
Cell Percentages nucleus (16%), cytoplasm (12%), mixed (50%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ela1

Ela1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ela1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available