Standard name
Human Ortholog
Description NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0.05 0 0.05 0 0.05 0 0.05 0 0 0.07 0.12 0.05 0 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.07 0 0 0 0 0 0 0.21 0.14 0.14 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.93 0.95 0.96 0.93 0.94 0.95 0.92 0.92 0.84 0.87 0.88 0.89 0.96 0.92 0.86 0.91 0.95 0.92 0.93 0.95 0.93 0.9 0.85 0.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0.12 0.1 0.12 0.12 0 0 0 0 0 0 0 0 0 0 0 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Bud 1 1 0 0 0 0 0 0 1 2 0 1 0 0 0 0 0 0 2 4 0 1 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 0 0 0 2 6 3 3 2 0 0 0 0 1 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 5 5 9 10 7 12 14 14 7 9 3 3 12 37 13 9 11 0 0 0 0 2 1
Endoplasmic Reticulum 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 3 3 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 1 2 0
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 0 0 0
Mitochondria 4 26 0 0 2 5 6 11 62 41 27 26 1 1 2 5 3 2 0 4 2 0 0 3
Nucleus 137 338 176 137 184 199 234 289 252 259 173 217 108 156 263 244 357 207 150 347 181 89 181 131
Nuclear Periphery 0 2 0 0 3 1 1 1 1 3 0 0 0 0 1 2 1 2 0 0 0 0 0 1
Nucleolus 6 5 1 2 5 6 5 11 37 30 23 30 5 0 1 5 8 7 0 0 1 0 3 9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 2 0 0 1 1 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 4 2 1 0 1 3 6 4 3 0 3 0 0 2 9 3 2 3 5 4 4 18 35
Unique Cell Count 147 355 184 147 196 209 253 313 300 298 196 244 113 169 305 267 375 226 162 368 196 99 213 189
Labelled Cell Count 154 381 184 151 207 219 261 335 378 348 236 284 117 169 307 282 385 236 162 368 196 99 213 189


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.4 6.6 8.5 8.1 8.5 7.0 6.6 6.9 5.7 6.0 5.8 5.9 7.1 7.9 8.0 10.0 12.4 12.9 8.5 9.0 8.6
Std Deviation (1e-4) 2.6 1.2 1.4 1.6 2.0 1.7 1.6 1.6 1.5 1.2 1.5 1.4 1.8 1.8 2.1 2.4 3.1 4.1 1.5 1.9 1.8
Intensity Change (Log2) – – – -0.08 -0.0 -0.29 -0.37 -0.31 -0.59 -0.52 -0.56 -0.52 -0.27 -0.1 -0.1 0.23 0.53 0.6 -0.0 0.07 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.5 1.2 0.4 1.1 1.0 1.1 0 1.0 0 0 1.9 3.6 1.1 0 1.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 6.6 5.3 5.2 4.6 0 0 0 0 0 0
Nucleus -1.0 -0.8 -0.2 -1.4 -1.5 -3.9 -3.1 -2.6 -2.5 0 -1.3 -3.3 -1.8 -0.2 -1.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 4.7 4.1 4.5 4.6 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.1233 7.5513 6.4417 6.2142 6.0536 6.8366 10.1834 9.1268 7.4032 6.611 5.927 7.4579 3.9644 4.7771 4.1202 4.3674 4.2654 4.0335
Actin 0.0049 0 0.0201 0.0301 0.0002 0.0001 0.002 0 0.0137 0.0438 0.0371 0.002 0.0489 0 0.0136 0.0019 0.0007 0.0039
Bud 0.0003 0 0.0001 0.0008 0.0002 0.0001 0.0003 0 0.0005 0 0.0005 0.0003 0.0003 0 0.0003 0.0001 0 0.0001
Bud Neck 0.0022 0 0.0003 0.008 0.0002 0.0007 0.001 0 0.0002 0.0002 0.0005 0.0007 0.0027 0 0.0004 0.0002 0.0001 0.0003
Bud Periphery 0.0004 0 0.0003 0.0015 0.0002 0.0002 0.0006 0 0.0005 0 0.0015 0.0004 0.0004 0 0.0005 0.0001 0 0.0003
Bud Site 0.0029 0 0.0008 0.0056 0.0015 0.0001 0.0036 0 0.0048 0.0002 0.0012 0.0006 0.0018 0 0.0018 0.0002 0 0.0005
Cell Periphery 0.0002 0 0.0001 0.001 0.0001 0 0.0033 0 0.0001 0 0.0012 0 0.0002 0 0.0002 0 0 0.0001
Cytoplasm 0.0022 0 0.0018 0.0012 0.0008 0.0001 0.0265 0 0.0026 0.0001 0.0184 0.0004 0.0024 0 0.0009 0.0003 0 0.0001
Cytoplasmic Foci 0.0111 0 0.0061 0.0148 0.01 0 0.0115 0 0.0051 0.0039 0.0276 0.0008 0.0196 0 0.0055 0.0027 0.0009 0.001
Eisosomes 0.0001 0 0.0005 0.0003 0 0 0.0001 0 0.0001 0.0001 0.0003 0 0.0004 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0014 0 0.0009 0.0018 0.0003 0 0.0084 0 0.0037 0.0001 0.0094 0.0005 0.0028 0 0.0028 0.0004 0 0.0001
Endosome 0.0072 0 0.0043 0.0221 0.0787 0.0001 0.0227 0 0.0047 0.0005 0.0342 0.0033 0.0123 0 0.0043 0.0034 0.0004 0.004
Golgi 0.0026 0 0.0016 0.008 0.0118 0 0.0021 0 0.0019 0.0019 0.0088 0.0009 0.0048 0 0.0033 0.0044 0.0016 0.0016
Lipid Particles 0.0085 0 0.0057 0.0322 0.0059 0 0.0209 0 0.0013 0.0039 0.0156 0.0012 0.0098 0 0.0048 0.0142 0.0313 0.0039
Mitochondria 0.0032 0.0002 0.0007 0.0062 0.0034 0.0014 0.0044 0.0001 0.0016 0.0002 0.0136 0.0027 0.0012 0.0001 0.0029 0.0059 0.0019 0.0019
None 0.0037 0 0.0111 0.001 0.0001 0 0.0076 0 0.0016 0.0002 0.022 0.0001 0.0035 0 0.0024 0.0002 0 0.0002
Nuclear Periphery 0.0098 0.0011 0.0021 0.0061 0.0018 0.0006 0.0352 0.0003 0.0098 0.0002 0.0339 0.0026 0.0106 0.0005 0.0064 0.0095 0.002 0.0005
Nucleolus 0.0079 0.0038 0.0055 0.0039 0.0185 0.024 0.0174 0.0025 0.0034 0.0135 0.0356 0.0181 0.0086 0.0049 0.0051 0.0077 0.0226 0.0121
Nucleus 0.9032 0.9947 0.9305 0.8321 0.8546 0.972 0.808 0.997 0.9353 0.9195 0.7091 0.9627 0.8348 0.9944 0.937 0.9342 0.9247 0.9646
Peroxisomes 0.0117 0 0.0044 0.0023 0.0019 0 0.0013 0 0.0007 0.011 0.0133 0.0009 0.0065 0 0.0018 0.0055 0.013 0.0026
Punctate Nuclear 0.0148 0.0001 0.0025 0.003 0.0008 0.0001 0.0102 0 0.0076 0.0005 0.0032 0.0006 0.026 0 0.0033 0.0082 0.0005 0.001
Vacuole 0.001 0 0.0002 0.0148 0.0069 0.0002 0.0093 0 0.0003 0 0.0083 0.0007 0.0015 0 0.0009 0.0003 0 0.0009
Vacuole Periphery 0.0007 0.0001 0.0002 0.0032 0.002 0.0002 0.0036 0 0.0003 0 0.0047 0.0005 0.0008 0 0.0014 0.0004 0.0001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.2888 29.7774 47.8749 25.6868 34.1907 20.8074 31.9896 22.4375 33.1478 28.704
Translational Efficiency 1.6687 1.384 0.7554 1.7496 1.318 1.8081 1.1299 1.5255 1.1971 1.4671

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
267 331 1501 1459 1369 2274 466 865 1636 2605 1967 2324

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 995.14 1079.89 1184.06 1174.81 784.26 1039.78 1144.70 1239.02 818.68 1044.88 1174.74 1198.71
Standard Deviation 116.86 160.61 221.73 221.14 144.19 138.69 229.57 224.66 160.31 142.29 224.24 224.61
Intensity Change Log 2 — 0.117913 0.250771 0.239456 — 0.406874 0.545566 0.659796 — 0.252449 0.388171 0.439933

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000438 0.000557 0.000236 0.000939 0.000455 0.000307 0.000623 0.000884 0.000453 0.000339 0.000328 0.000918
Bud Neck 0.001202 0.012263 0.007609 0.021671 0.003043 0.006512 0.005878 0.020954 0.002743 0.007242 0.007199 0.021405
Bud Site 0.001166 0.001133 0.000288 0.003314 0.001108 0.001002 0.002378 0.002552 0.001117 0.001019 0.000783 0.003030
Cell Periphery 0.000028 0.000136 0.000192 0.000180 0.000324 0.000308 0.000087 0.000135 0.000276 0.000287 0.000167 0.000163
Cytoplasm 0.000415 0.000624 0.000115 0.002923 0.004455 0.000451 0.000986 0.002360 0.003796 0.000473 0.000321 0.002713
Cytoplasmic Foci 0.000096 0.000046 0.000002 0.000461 0.000314 0.000223 0.000223 0.000883 0.000278 0.000201 0.000054 0.000618
Eisosomes 0.000018 0.000034 0.000083 0.000027 0.000027 0.000058 0.000050 0.000039 0.000026 0.000055 0.000075 0.000032
Endoplasmic Reticulum 0.004443 0.006538 0.002307 0.002712 0.006649 0.008809 0.003485 0.002830 0.006289 0.008520 0.002586 0.002756
Endosome 0.000084 0.000316 0.000024 0.002175 0.000281 0.000500 0.000096 0.002435 0.000249 0.000477 0.000041 0.002272
Golgi 0.000022 0.000109 0.000008 0.000617 0.000083 0.000256 0.000040 0.002243 0.000073 0.000237 0.000015 0.001222
Lipid Particles 0.000138 0.000066 0.000001 0.000344 0.000067 0.000073 0.000064 0.000099 0.000078 0.000072 0.000016 0.000253
Mitochondria 0.001206 0.001132 0.000404 0.003431 0.000779 0.001764 0.000862 0.003629 0.000849 0.001683 0.000512 0.003504
Mitotic Spindle 0.001131 0.004075 0.000036 0.009669 0.000549 0.002779 0.000788 0.008034 0.000644 0.002944 0.000214 0.009060
None 0.000537 0.001160 0.000422 0.002066 0.009041 0.001117 0.004076 0.002426 0.007653 0.001122 0.001288 0.002200
Nuclear Periphery 0.000193 0.000352 0.000091 0.001808 0.000303 0.000668 0.000145 0.001052 0.000285 0.000628 0.000104 0.001527
Nuclear Periphery Foci 0.000080 0.000127 0.000009 0.000904 0.000493 0.000269 0.000128 0.000647 0.000426 0.000251 0.000037 0.000808
Nucleolus 0.005872 0.009947 0.001921 0.006017 0.009544 0.006745 0.007046 0.008465 0.008944 0.007152 0.003135 0.006928
Nucleus 0.982103* 0.959154* 0.985665* 0.934623* 0.961347* 0.965425* 0.971939* 0.933241* 0.964735* 0.964628* 0.982413* 0.934108*
Peroxisomes 0.000274 0.000418 0.000034 0.000314 0.000216 0.000177 0.000343 0.000285 0.000225 0.000207 0.000108 0.000303
Vacuole 0.000374 0.001337 0.000494 0.004075 0.000748 0.001841 0.000705 0.004674 0.000687 0.001777 0.000544 0.004298
Vacuole Periphery 0.000180 0.000478 0.000060 0.001731 0.000174 0.000716 0.000059 0.002133 0.000175 0.000686 0.000059 0.001881

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.80 1.04 -2.78 -3.44 -6.55 0.96 -1.41 -4.05 -9.38 -5.06 0.81 0.88 -4.23 -8.25 -8.74
Bud Neck -4.93 -14.45 -13.67 -3.09 -9.76 -5.40 -5.75 -11.18 -9.05 -9.14 -7.30 -11.68 -16.78 -12.00 -13.05
Bud Site -0.14 0.85 -2.05 -3.68 -6.58 0.21 -0.88 -3.89 -4.23 -0.44 0.16 0.59 -4.70 -5.26 -5.08
Cell Periphery -1.59 -4.73 -3.76 -0.25 0.74 0.28 3.85 3.45 4.02 -1.07 -0.14 2.11 2.62 3.30 0.64
Cytoplasm -0.67 1.07 -3.99 -3.79 -4.51 3.87 3.10 1.61 -2.41 -1.56 3.82 4.00 0.38 -4.69 -4.93
Cytoplasmic Foci 0.69 1.44 -1.95 -2.69 -3.17 0.89 0.45 -0.58 -1.36 -0.97 0.88 1.62 -0.31 -1.58 -2.75
Eisosomes -4.83 -7.83 -5.88 1.51 6.46 -5.93 -5.40 -3.52 3.35 2.57 -6.53 -7.38 -2.88 5.34 6.52
Endoplasmic Reticulum -3.08 4.26 2.70 6.21 -2.21 -4.17 7.65 7.89 11.75 0.93 -4.91 11.36 9.10 13.67 -2.04
Endosome -1.84 1.81 -3.07 -2.35 -3.19 -1.55 3.37 -2.19 -1.86 -2.45 -1.91 5.21 -3.52 -2.87 -4.05
Golgi -1.69 2.67 -3.51 -2.64 -3.61 -1.36 1.24 -1.85 -1.63 -1.90 -1.49 2.17 -2.58 -2.00 -2.78
Lipid Particles 0.72 1.61 -0.72 -1.14 -1.55 -0.08 0.24 -1.79 -1.59 -2.24 0.27 2.99 -1.22 -1.29 -1.82
Mitochondria -0.02 1.06 -2.28 -3.48 -5.23 -1.90 -0.73 -5.01 -2.62 -5.00 -1.81 1.60 -5.80 -3.01 -7.21
Mitotic Spindle -1.14 1.00 -4.45 -2.29 -5.49 -2.47 -0.64 -3.67 -2.69 -3.43 -2.68 1.25 -6.28 -4.40 -6.55
None -1.28 0.90 -6.67 -2.30 -7.16 5.20 2.81 4.34 -2.73 1.24 5.10 4.97 4.16 -4.02 -3.18
Nuclear Periphery -1.20 0.77 -3.43 -2.75 -3.78 -2.53 2.61 -3.39 -1.70 -3.98 -2.75 3.86 -4.26 -2.75 -4.92
Nuclear Periphery Foci -0.95 1.88 -2.86 -2.40 -3.20 0.90 1.15 -0.37 -1.34 -1.55 0.81 2.00 -0.93 -2.15 -3.76
Nucleolus -2.18 4.21 -1.18 1.41 -5.17 2.63 2.02 -1.66 -3.25 -2.93 1.85 7.03 -0.28 -1.95 -6.43
Nucleus 3.70 -0.78 8.86 3.64 12.22 -1.23 -2.15 5.76 6.75 6.96 0.08 -6.42 8.58 9.09 14.74
Peroxisomes -1.07 3.58 -0.28 0.83 -3.27 0.45 -1.57 -1.80 -3.30 -0.27 0.20 1.74 -1.20 -1.79 -3.94
Vacuole -2.83 -0.73 -4.67 -2.76 -4.56 -3.40 0.03 -3.49 -2.62 -3.48 -3.96 1.21 -5.51 -3.71 -5.74
Vacuole Periphery -1.26 1.02 -2.94 -1.93 -3.41 -3.06 2.72 -2.25 -1.40 -2.43 -3.25 2.92 -3.74 -2.07 -4.10
Standard name
Human Ortholog
Description NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Vps75

Vps75


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vps75-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available