Standard name | |
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Human Ortholog | |
Description | Putative sterol transfer protein; has a probable role in retrograde transport of sterols from the plasma membrane to the ER; co-localizes to puncta in the cortical ER with Ysp2p; contains GRAM, StART-like (VASt) and two PH-like domains; one of 6 StART-like domain-containing proteins in yeast that may be involved in sterol transfer between intracellular membranes; conserved across eukaryotes; previously identified as a transcription cofactor that interacts with DNA-bound Snf1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | – | – | – | 0 | – | 0 | – | – | – | 1 | 0 | 3 | 1 | 4 | 3 | 3 | 31 | 2 | 5 | 17 | 26 | 55 |
Bud | 0 | 0 | – | – | – | 1 | – | 3 | – | – | – | 5 | 1 | 5 | 10 | 6 | 2 | 1 | 1 | 1 | 0 | 1 | 3 | 3 |
Bud Neck | 4 | 0 | – | – | – | 0 | – | 0 | – | – | – | 1 | 1 | 0 | 0 | 4 | 2 | 0 | 10 | 1 | 0 | 8 | 6 | 15 |
Bud Site | 0 | 0 | – | – | – | 0 | – | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 7 | 2 | – | – | – | 2 | – | 4 | – | – | – | 2 | 5 | 1 | 7 | 9 | 10 | 10 | 22 | 9 | 2 | 16 | 24 | 42 |
Cytoplasm | 168 | 45 | – | – | – | 31 | – | 69 | – | – | – | 47 | 134 | 233 | 216 | 139 | 79 | 90 | 74 | 43 | 14 | 50 | 88 | 75 |
Endoplasmic Reticulum | 1 | 2 | – | – | – | 1 | – | 2 | – | – | – | 0 | 0 | 0 | 0 | 13 | 1 | 7 | 9 | 0 | 0 | 1 | 1 | 4 |
Endosome | 0 | 0 | – | – | – | 0 | – | 1 | – | – | – | 0 | 0 | 0 | 0 | 3 | 5 | 1 | 5 | 2 | 0 | 4 | 2 | 4 |
Golgi | 1 | 0 | – | – | – | 0 | – | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 1 |
Mitochondria | 1 | 0 | – | – | – | 7 | – | 29 | – | – | – | 46 | 2 | 2 | 6 | 8 | 1 | 6 | 47 | 9 | 13 | 14 | 21 | 38 |
Nucleus | 4 | 0 | – | – | – | 0 | – | 0 | – | – | – | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 13 | 0 | – | – | – | 0 | – | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | – | – | – | 0 | – | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | – | – | – | 0 | – | 0 | – | – | – | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 1 | 0 | – | – | – | 0 | – | 1 | – | – | – | 3 | 0 | 0 | 0 | 6 | 5 | 7 | 1 | 0 | 0 | 1 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | – | – | – | 0 | – | 0 | – | – | – | 0 | 1 | 0 | 0 | 2 | 6 | 0 | 6 | 1 | 0 | 2 | 4 | 7 |
Unique Cell Count | 189 | 47 | 37 | 92 | 79 | 138 | 241 | 221 | 165 | 100 | 107 | 218 | 81 | 39 | 123 | 186 | 257 | |||||||
Labelled Cell Count | 201 | 50 | 42 | 109 | 106 | 144 | 245 | 240 | 195 | 119 | 127 | 218 | 81 | 39 | 123 | 186 | 257 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 3.9 | – | – | – | 3.2 | – | 3.1 | – | – | – | 2.8 | 3.9 | 4.2 | 3.7 | 5.8 | 6.9 | 6.0 | 4.1 | 4.2 | 4.6 |
Std Deviation (1e-4) | 0.7 | 1.3 | – | – | – | 1.0 | – | 1.2 | – | – | – | 1.5 | 1.1 | 1.1 | 1.2 | 2.1 | 4.6 | 2.0 | 1.3 | 1.6 | 1.5 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.6489 | 2.0495 | 1.9582 | 1.5822 | 1.1377 | 1.9796 | 0.8998 | 1.1158 | 1.3226 | 1.1211 | 0.64 | 1.4084 | 0.8708 | 1.3901 | 1.4039 | 1.0057 | 0.8307 | 1.7512 |
Actin | 0.0015 | 0.0024 | 0.0146 | 0.0009 | 0.0002 | 0.0022 | 0.0593 | 0.0079 | 0.0086 | 0.0009 | 0.0067 | 0.001 | 0.1362 | 0.0073 | 0.0017 | 0.0034 | 0.0002 | 0.007 |
Bud | 0.0028 | 0.0006 | 0.0009 | 0.0003 | 0 | 0.0001 | 0.0003 | 0.0019 | 0.0005 | 0.0003 | 0.0002 | 0 | 0.0029 | 0.0063 | 0.0008 | 0.0001 | 0 | 0.0005 |
Bud Neck | 0.0055 | 0.0016 | 0.0179 | 0.0035 | 0.0003 | 0.0027 | 0.0104 | 0.0021 | 0.0106 | 0.0048 | 0.0386 | 0.0024 | 0.0013 | 0.001 | 0.0041 | 0.0163 | 0.0001 | 0.0231 |
Bud Periphery | 0.0061 | 0.0017 | 0.0048 | 0.001 | 0.0002 | 0.0003 | 0.0009 | 0.0035 | 0.0017 | 0.0003 | 0.0012 | 0.0001 | 0.0071 | 0.0314 | 0.0019 | 0.0004 | 0 | 0.0022 |
Bud Site | 0.0419 | 0.0354 | 0.0155 | 0.0021 | 0.0001 | 0.0001 | 0.0068 | 0.0377 | 0.0397 | 0.0026 | 0.0022 | 0.0001 | 0.1063 | 0.0667 | 0.0095 | 0.0005 | 0 | 0.0004 |
Cell Periphery | 0.0141 | 0.0128 | 0.0194 | 0.0096 | 0.0027 | 0.0014 | 0.0043 | 0.0142 | 0.0096 | 0.003 | 0.0091 | 0.0005 | 0.0027 | 0.0025 | 0.0038 | 0.0047 | 0.0001 | 0.0019 |
Cytoplasm | 0.4795 | 0.4997 | 0.5406 | 0.6041 | 0.4284 | 0.6051 | 0.1446 | 0.3903 | 0.4141 | 0.3909 | 0.3603 | 0.5256 | 0.1795 | 0.2677 | 0.179 | 0.3131 | 0.2766 | 0.3482 |
Cytoplasmic Foci | 0.0199 | 0.0093 | 0.005 | 0.0056 | 0.0013 | 0.0058 | 0.0208 | 0.011 | 0.0123 | 0.0245 | 0.0393 | 0.0127 | 0.0471 | 0.0084 | 0.0053 | 0.0218 | 0.0024 | 0.0038 |
Eisosomes | 0.0017 | 0.0005 | 0.0006 | 0.0002 | 0.0002 | 0.0002 | 0.0008 | 0.0008 | 0.0005 | 0.0003 | 0.0006 | 0 | 0.0005 | 0.0008 | 0.0006 | 0.0014 | 0 | 0.0003 |
Endoplasmic Reticulum | 0.0034 | 0.005 | 0.0031 | 0.0035 | 0.0013 | 0.0019 | 0.0094 | 0.0018 | 0.0024 | 0.0051 | 0.0007 | 0.001 | 0.013 | 0.002 | 0.0023 | 0.0026 | 0.0006 | 0.0012 |
Endosome | 0.0029 | 0.0009 | 0.0004 | 0.0011 | 0.0001 | 0.0009 | 0.0147 | 0.0052 | 0.0076 | 0.018 | 0.0003 | 0.0111 | 0.0238 | 0.0005 | 0.0228 | 0.0007 | 0.0004 | 0.0016 |
Golgi | 0.0013 | 0.0005 | 0.0005 | 0.0004 | 0 | 0.0002 | 0.0138 | 0.0005 | 0.0144 | 0.0025 | 0.0005 | 0.002 | 0.015 | 0.0003 | 0.0006 | 0.0005 | 0 | 0.0002 |
Lipid Particles | 0.0054 | 0.001 | 0.0009 | 0.0012 | 0.0002 | 0.0007 | 0.0345 | 0.0004 | 0.0014 | 0.0005 | 0.0002 | 0.0054 | 0.1236 | 0.0004 | 0.0017 | 0.0008 | 0 | 0.0001 |
Mitochondria | 0.0017 | 0.0002 | 0.0003 | 0.0002 | 0 | 0.0001 | 0.0024 | 0.0002 | 0.0037 | 0.0003 | 0.0002 | 0.0013 | 0.0028 | 0.0008 | 0.0004 | 0.0002 | 0.0001 | 0.0002 |
None | 0.4042 | 0.4253 | 0.3731 | 0.3637 | 0.5642 | 0.3771 | 0.6385 | 0.5181 | 0.4596 | 0.5417 | 0.5377 | 0.4121 | 0.2917 | 0.6011 | 0.7348 | 0.632 | 0.7189 | 0.6079 |
Nuclear Periphery | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0164 | 0.0002 | 0.0004 | 0.0003 | 0 | 0.0126 | 0.0012 | 0.0002 | 0.0007 | 0.0002 | 0.0001 | 0.0001 |
Nucleolus | 0.0009 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0014 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0003 | 0.0008 | 0.0003 | 0.0003 | 0.0001 | 0 | 0.0001 |
Nucleus | 0.0012 | 0.0006 | 0.0005 | 0.0004 | 0.0002 | 0.0003 | 0.0086 | 0.0005 | 0.0013 | 0.0006 | 0.0002 | 0.0042 | 0.0006 | 0.0004 | 0.0006 | 0.0003 | 0.0001 | 0.0003 |
Peroxisomes | 0.003 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0082 | 0.0003 | 0.0069 | 0.0003 | 0.001 | 0.0026 | 0.0387 | 0.0009 | 0.0003 | 0.0002 | 0 | 0.0001 |
Punctate Nuclear | 0.0009 | 0.0004 | 0.0004 | 0.0002 | 0 | 0.0002 | 0.0017 | 0.0002 | 0.0005 | 0.0003 | 0.0004 | 0.0041 | 0.004 | 0.0005 | 0.0006 | 0.0003 | 0.0001 | 0.0001 |
Vacuole | 0.0018 | 0.0016 | 0.0011 | 0.0015 | 0.0006 | 0.0004 | 0.0016 | 0.003 | 0.0035 | 0.0023 | 0.0005 | 0.0004 | 0.0009 | 0.0005 | 0.0272 | 0.0005 | 0.0003 | 0.0005 |
Vacuole Periphery | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0003 | 0.0004 | 0 | 0.001 | 0 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 24.6696 | 11.4944 | 12.8931 | 18.7919 | 16.8649 | 15.8396 | 21.6898 | 21.6167 | 18.1873 | 24.4824 |
Translational Efficiency | 0.2215 | 0.432 | 0.3582 | 0.2663 | 0.2956 | 0.3537 | 0.284 | 0.2117 | 0.3218 | 0.2658 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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