Standard name
Human Ortholog
Description Putative sterol transfer protein; has a probable role in retrograde transport of sterols from the plasma membrane to the ER; co-localizes to puncta in the cortical ER with Ysp2p; contains GRAM, StART-like (VASt) and two PH-like domains; one of 6 StART-like domain-containing proteins in yeast that may be involved in sterol transfer between intracellular membranes; conserved across eukaryotes; previously identified as a transcription cofactor that interacts with DNA-bound Snf1p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0.14 0 0.15 0.14 0.14 0.22
Bud 0 0 0 0 0.06 0 0 0.05 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.07 0 0.06
Bud Site 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.05 0 0 0 0 0 0.05 0.1 0.09 0.1 0.12 0.05 0.14 0.13 0.17
Cytoplasm 0.89 0.96 0.84 0.75 0.59 0.97 0.97 0.98 0.84 0.79 0.84 0.34 0.53 0.36 0.41 0.47 0.29
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0.08 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.19 0.32 0.58 0 0 0 0.05 0 0.06 0.22 0.12 0.36 0.12 0.12 0.15
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 1 0 3 1 4 3 3 31 2 5 17 26 55
Bud 0 0 1 3 5 1 5 10 6 2 1 1 1 0 1 3 3
Bud Neck 4 0 0 0 1 1 0 0 4 2 0 10 1 0 8 6 15
Bud Site 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 7 2 2 4 2 5 1 7 9 10 10 22 9 2 16 24 42
Cytoplasm 168 45 31 69 47 134 233 216 139 79 90 74 43 14 50 88 75
Endoplasmic Reticulum 1 2 1 2 0 0 0 0 13 1 7 9 0 0 1 1 4
Endosome 0 0 0 1 0 0 0 0 3 5 1 5 2 0 4 2 4
Golgi 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 1
Mitochondria 1 0 7 29 46 2 2 6 8 1 6 47 9 13 14 21 38
Nucleus 4 0 0 0 1 0 0 0 1 2 0 0 0 0 0 0 0
Nuclear Periphery 13 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 0 0 2 2 0 0 0 0 0 1
SpindlePole 1 0 0 1 3 0 0 0 6 5 7 1 0 0 1 0 0
Vac/Vac Membrane 0 1 0 0 0 1 0 0 2 6 0 6 1 0 2 4 7
Unique Cell Count 189 47 37 92 79 138 241 221 165 100 107 218 81 39 123 186 257
Labelled Cell Count 201 50 42 109 106 144 245 240 195 119 127 218 81 39 123 186 257


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 3.9 3.2 3.1 2.8 3.9 4.2 3.7 5.8 6.9 6.0 4.1 4.2 4.6
Std Deviation (1e-4) 0.7 1.3 1.0 1.2 1.5 1.1 1.1 1.2 2.1 4.6 2.0 1.3 1.6 1.5
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6489 2.0495 1.9582 1.5822 1.1377 1.9796 0.8998 1.1158 1.3226 1.1211 0.64 1.4084 0.8708 1.3901 1.4039 1.0057 0.8307 1.7512
Actin 0.0015 0.0024 0.0146 0.0009 0.0002 0.0022 0.0593 0.0079 0.0086 0.0009 0.0067 0.001 0.1362 0.0073 0.0017 0.0034 0.0002 0.007
Bud 0.0028 0.0006 0.0009 0.0003 0 0.0001 0.0003 0.0019 0.0005 0.0003 0.0002 0 0.0029 0.0063 0.0008 0.0001 0 0.0005
Bud Neck 0.0055 0.0016 0.0179 0.0035 0.0003 0.0027 0.0104 0.0021 0.0106 0.0048 0.0386 0.0024 0.0013 0.001 0.0041 0.0163 0.0001 0.0231
Bud Periphery 0.0061 0.0017 0.0048 0.001 0.0002 0.0003 0.0009 0.0035 0.0017 0.0003 0.0012 0.0001 0.0071 0.0314 0.0019 0.0004 0 0.0022
Bud Site 0.0419 0.0354 0.0155 0.0021 0.0001 0.0001 0.0068 0.0377 0.0397 0.0026 0.0022 0.0001 0.1063 0.0667 0.0095 0.0005 0 0.0004
Cell Periphery 0.0141 0.0128 0.0194 0.0096 0.0027 0.0014 0.0043 0.0142 0.0096 0.003 0.0091 0.0005 0.0027 0.0025 0.0038 0.0047 0.0001 0.0019
Cytoplasm 0.4795 0.4997 0.5406 0.6041 0.4284 0.6051 0.1446 0.3903 0.4141 0.3909 0.3603 0.5256 0.1795 0.2677 0.179 0.3131 0.2766 0.3482
Cytoplasmic Foci 0.0199 0.0093 0.005 0.0056 0.0013 0.0058 0.0208 0.011 0.0123 0.0245 0.0393 0.0127 0.0471 0.0084 0.0053 0.0218 0.0024 0.0038
Eisosomes 0.0017 0.0005 0.0006 0.0002 0.0002 0.0002 0.0008 0.0008 0.0005 0.0003 0.0006 0 0.0005 0.0008 0.0006 0.0014 0 0.0003
Endoplasmic Reticulum 0.0034 0.005 0.0031 0.0035 0.0013 0.0019 0.0094 0.0018 0.0024 0.0051 0.0007 0.001 0.013 0.002 0.0023 0.0026 0.0006 0.0012
Endosome 0.0029 0.0009 0.0004 0.0011 0.0001 0.0009 0.0147 0.0052 0.0076 0.018 0.0003 0.0111 0.0238 0.0005 0.0228 0.0007 0.0004 0.0016
Golgi 0.0013 0.0005 0.0005 0.0004 0 0.0002 0.0138 0.0005 0.0144 0.0025 0.0005 0.002 0.015 0.0003 0.0006 0.0005 0 0.0002
Lipid Particles 0.0054 0.001 0.0009 0.0012 0.0002 0.0007 0.0345 0.0004 0.0014 0.0005 0.0002 0.0054 0.1236 0.0004 0.0017 0.0008 0 0.0001
Mitochondria 0.0017 0.0002 0.0003 0.0002 0 0.0001 0.0024 0.0002 0.0037 0.0003 0.0002 0.0013 0.0028 0.0008 0.0004 0.0002 0.0001 0.0002
None 0.4042 0.4253 0.3731 0.3637 0.5642 0.3771 0.6385 0.5181 0.4596 0.5417 0.5377 0.4121 0.2917 0.6011 0.7348 0.632 0.7189 0.6079
Nuclear Periphery 0.0002 0.0003 0.0002 0.0002 0.0001 0.0001 0.0164 0.0002 0.0004 0.0003 0 0.0126 0.0012 0.0002 0.0007 0.0002 0.0001 0.0001
Nucleolus 0.0009 0.0002 0.0001 0.0001 0.0001 0.0001 0.0014 0.0001 0.0005 0.0001 0.0001 0.0003 0.0008 0.0003 0.0003 0.0001 0 0.0001
Nucleus 0.0012 0.0006 0.0005 0.0004 0.0002 0.0003 0.0086 0.0005 0.0013 0.0006 0.0002 0.0042 0.0006 0.0004 0.0006 0.0003 0.0001 0.0003
Peroxisomes 0.003 0.0001 0.0002 0.0001 0 0.0001 0.0082 0.0003 0.0069 0.0003 0.001 0.0026 0.0387 0.0009 0.0003 0.0002 0 0.0001
Punctate Nuclear 0.0009 0.0004 0.0004 0.0002 0 0.0002 0.0017 0.0002 0.0005 0.0003 0.0004 0.0041 0.004 0.0005 0.0006 0.0003 0.0001 0.0001
Vacuole 0.0018 0.0016 0.0011 0.0015 0.0006 0.0004 0.0016 0.003 0.0035 0.0023 0.0005 0.0004 0.0009 0.0005 0.0272 0.0005 0.0003 0.0005
Vacuole Periphery 0.0001 0 0 0.0001 0 0 0.0005 0.0001 0.0002 0.0002 0 0.0003 0.0004 0 0.001 0 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.6696 11.4944 12.8931 18.7919 16.8649 15.8396 21.6898 21.6167 18.1873 24.4824
Translational Efficiency 0.2215 0.432 0.3582 0.2663 0.2956 0.3537 0.284 0.2117 0.3218 0.2658

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Putative sterol transfer protein; has a probable role in retrograde transport of sterols from the plasma membrane to the ER; co-localizes to puncta in the cortical ER with Ysp2p; contains GRAM, StART-like (VASt) and two PH-like domains; one of 6 StART-like domain-containing proteins in yeast that may be involved in sterol transfer between intracellular membranes; conserved across eukaryotes; previously identified as a transcription cofactor that interacts with DNA-bound Snf1p
Localization
Cell Percentages cytoplasm (16%), ER (5%), mixed (41%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Sip3

Sip3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sip3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available