Standard name | |
---|---|
Human Ortholog | |
Description | Pheromone-regulated multispanning membrane protein; involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 2 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | 1 | 2 | 1 | 9 | 57 | 104 | 47 |
Bud | 0 | 0 | 0 | 4 | 4 | 0 | – | 18 | 13 | 16 | 22 | 20 | 0 | 0 | 0 | – | – | 2 | 1 | 0 | 2 | 47 | 51 | 21 |
Bud Neck | 2 | 0 | 2 | 0 | 1 | 0 | – | 4 | 3 | 1 | 1 | 2 | 5 | 5 | 8 | – | – | 3 | 1 | 0 | 5 | 31 | 14 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 0 | 3 | 1 | 5 | 0 | 0 | 0 | – | – | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 3 | 0 | 1 | – | 0 | 1 | 1 | 0 | 3 | 11 | 2 | 1 | – | – | 0 | 1 | 1 | 3 | 0 | 0 | 0 |
Cytoplasm | 217 | 60 | 355 | 248 | 385 | 346 | – | 513 | 291 | 364 | 227 | 302 | 242 | 406 | 478 | – | – | 91 | 169 | 84 | 225 | 6 | 2 | 1 |
Endoplasmic Reticulum | 1 | 2 | 1 | 0 | 1 | 0 | – | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 1 | – | – | 7 | 2 | 2 | 7 | 88 | 153 | 141 |
Endosome | 1 | 0 | 1 | 2 | 25 | 5 | – | 10 | 3 | 1 | 2 | 4 | 0 | 0 | 1 | – | – | 1 | 2 | 6 | 11 | 7 | 79 | 108 |
Golgi | 0 | 0 | 0 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 2 | 2 | 79 | 107 |
Mitochondria | 1 | 11 | 10 | 20 | 20 | 112 | – | 222 | 203 | 309 | 296 | 365 | 0 | 1 | 0 | – | – | 9 | 19 | 29 | 94 | 10 | 15 | 11 |
Nucleus | 1 | 0 | 1 | 0 | 0 | 1 | – | 8 | 3 | 2 | 0 | 3 | 0 | 1 | 0 | – | – | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 1 | 0 | – | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 2 | 6 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | – | 5 | 1 | 1 | 2 | 1 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 1 | 3 | 11 | 4 | – | 3 | 0 | 3 | 2 | 4 | 0 | 0 | 0 | – | – | 2 | 0 | 0 | 1 | 0 | 3 | 0 |
SpindlePole | 0 | 1 | 1 | 4 | 8 | 8 | – | 26 | 17 | 22 | 15 | 41 | 0 | 0 | 0 | – | – | 2 | 1 | 2 | 5 | 6 | 3 | 0 |
Vac/Vac Membrane | 3 | 0 | 5 | 1 | 7 | 19 | – | 17 | 4 | 8 | 4 | 9 | 0 | 0 | 0 | – | – | 0 | 26 | 21 | 10 | 8 | 56 | 205 |
Unique Cell Count | 224 | 70 | 366 | 268 | 435 | 431 | 723 | 430 | 580 | 448 | 602 | 243 | 408 | 480 | 100 | 233 | 167 | 391 | 270 | 569 | 662 | |||
Labelled Cell Count | 226 | 75 | 378 | 286 | 466 | 498 | 831 | 539 | 731 | 574 | 760 | 259 | 416 | 490 | 122 | 233 | 167 | 391 | 270 | 569 | 662 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.8 | 3.4 | 3.6 | 3.6 | 3.8 | 3.1 | – | 3.2 | 3.0 | 2.5 | 2.8 | 2.7 | 4.5 | 4.8 | 4.9 | 3.7 | 3.9 | 6.0 | 11.5 | 16.8 | 20.2 |
Std Deviation (1e-4) | 0.7 | 0.7 | 1.1 | 1.5 | 1.9 | 1.5 | – | 1.0 | 1.4 | 0.7 | 1.0 | 0.6 | 0.7 | 1.0 | 1.0 | 1.3 | 1.2 | 9.8 | 4.1 | 5.1 | 5.1 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.08 | -0.19 | – | -0.19 | -0.28 | -0.51 | -0.38 | -0.4 | 0.32 | 0.41 | 0.45 | 0.03 | 0.13 | 0.73 | 1.68 | 2.23 | 2.49 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.1587 | -0.5354 | -0.3441 | -0.4182 | -0.6969 | -0.3003 | -0.2413 | 7.3958 | 0.567 | -0.5793 | -0.5852 | -0.3139 | -0.6795 | -0.252 | -0.4073 | -0.9411 | -0.4312 | -0.6661 |
Actin | 0.0552 | 0.0002 | 0.0176 | 0.0165 | 0.0305 | 0.0026 | 0.0147 | 0.0055 | 0.015 | 0.0001 | 0.0647 | 0.0007 | 0.0456 | 0.0002 | 0.0364 | 0.0016 | 0.0267 | 0.0075 |
Bud | 0.0089 | 0.0012 | 0.0011 | 0.0028 | 0.0335 | 0.0021 | 0.0003 | 0.0011 | 0.002 | 0 | 0.0036 | 0 | 0.0004 | 0.0012 | 0.0023 | 0.0013 | 0.0006 | 0 |
Bud Neck | 0.0078 | 0.0002 | 0.0052 | 0.0027 | 0.0005 | 0.0016 | 0.001 | 0.0024 | 0.0027 | 0 | 0.0011 | 0.0001 | 0.002 | 0.0001 | 0.0011 | 0.0001 | 0.0015 | 0.0003 |
Bud Periphery | 0.0168 | 0.0008 | 0.0028 | 0.0042 | 0.0097 | 0.0054 | 0.0008 | 0.0019 | 0.0035 | 0 | 0.0164 | 0 | 0.0012 | 0.0015 | 0.0049 | 0.0067 | 0.001 | 0.0001 |
Bud Site | 0.0069 | 0.016 | 0.0107 | 0.0107 | 0.0038 | 0.0002 | 0.0031 | 0.0447 | 0.0125 | 0 | 0.0021 | 0 | 0.0027 | 0.008 | 0.0041 | 0.0001 | 0.009 | 0 |
Cell Periphery | 0.0013 | 0.0004 | 0.0002 | 0.0004 | 0.0004 | 0.0002 | 0.0008 | 0.0103 | 0.0019 | 0 | 0.0006 | 0 | 0.0003 | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0 |
Cytoplasm | 0.0231 | 0.0289 | 0.0304 | 0.0408 | 0.0114 | 0.0426 | 0.0528 | 0.071 | 0.0501 | 0.0301 | 0.077 | 0.0519 | 0.0588 | 0.0617 | 0.0427 | 0.0598 | 0.0684 | 0.1082 |
Cytoplasmic Foci | 0.029 | 0.0251 | 0.014 | 0.0143 | 0.0399 | 0.0162 | 0.0226 | 0.0065 | 0.0113 | 0.0047 | 0.0149 | 0.0049 | 0.025 | 0.0068 | 0.0161 | 0.0029 | 0.0153 | 0.0113 |
Eisosomes | 0.001 | 0.0003 | 0.0005 | 0.0003 | 0.0004 | 0.0001 | 0.0053 | 0.0007 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0004 | 0.0003 | 0.0003 | 0.0001 |
Endoplasmic Reticulum | 0.0055 | 0.001 | 0.0021 | 0.0015 | 0.0043 | 0.0005 | 0.0032 | 0.0023 | 0.0009 | 0.0004 | 0.0019 | 0.0005 | 0.0029 | 0.0013 | 0.004 | 0.001 | 0.0019 | 0.0004 |
Endosome | 0.0199 | 0.0013 | 0.0045 | 0.0066 | 0.0081 | 0.0048 | 0.0156 | 0.0039 | 0.0169 | 0.0005 | 0.0095 | 0.0009 | 0.0086 | 0.0128 | 0.0056 | 0.0008 | 0.0204 | 0.0027 |
Golgi | 0.0119 | 0.0002 | 0.0026 | 0.0039 | 0.0103 | 0.0008 | 0.0021 | 0.0003 | 0.0013 | 0 | 0.0072 | 0.0001 | 0.0044 | 0.0003 | 0.0033 | 0.0002 | 0.0081 | 0.0001 |
Lipid Particles | 0.0239 | 0.0007 | 0.0047 | 0.0066 | 0.0228 | 0.0023 | 0.0054 | 0.0024 | 0.0035 | 0.0005 | 0.0155 | 0.0002 | 0.0115 | 0.0005 | 0.0079 | 0.0003 | 0.0119 | 0.0002 |
Mitochondria | 0.0112 | 0.0003 | 0.0039 | 0.0069 | 0.0051 | 0.0019 | 0.0073 | 0.0011 | 0.0011 | 0.0001 | 0.0173 | 0.0001 | 0.0018 | 0.0004 | 0.0026 | 0.0014 | 0.0044 | 0.0002 |
None | 0.7306 | 0.9166 | 0.8865 | 0.7897 | 0.7861 | 0.9012 | 0.842 | 0.8265 | 0.8632 | 0.9626 | 0.7406 | 0.924 | 0.7953 | 0.8949 | 0.8498 | 0.9193 | 0.791 | 0.8631 |
Nuclear Periphery | 0.0072 | 0.0003 | 0.0014 | 0.0038 | 0.0055 | 0.0012 | 0.0051 | 0.0009 | 0.0003 | 0.0002 | 0.001 | 0.0001 | 0.0046 | 0.0004 | 0.0029 | 0.0009 | 0.0073 | 0.0002 |
Nucleolus | 0.0015 | 0.0004 | 0.0009 | 0.0018 | 0.0004 | 0.0009 | 0.0008 | 0.0034 | 0.0004 | 0 | 0.001 | 0 | 0.0016 | 0.0003 | 0.0006 | 0.0002 | 0.0015 | 0 |
Nucleus | 0.0022 | 0.0006 | 0.0009 | 0.0115 | 0.0009 | 0.0029 | 0.002 | 0.0056 | 0.0015 | 0.0001 | 0.0015 | 0.0001 | 0.0045 | 0.0007 | 0.001 | 0.0005 | 0.0027 | 0.0003 |
Peroxisomes | 0.0276 | 0.0014 | 0.0046 | 0.0437 | 0.0188 | 0.0022 | 0.0077 | 0.0004 | 0.0061 | 0.0001 | 0.0086 | 0.0001 | 0.021 | 0.0002 | 0.008 | 0.0005 | 0.0134 | 0.0003 |
Punctate Nuclear | 0.0056 | 0.0028 | 0.0046 | 0.0276 | 0.0058 | 0.008 | 0.0038 | 0.0019 | 0.0008 | 0.0002 | 0.001 | 0.0001 | 0.0039 | 0.0004 | 0.0035 | 0.0004 | 0.0124 | 0.0004 |
Vacuole | 0.0019 | 0.0012 | 0.0007 | 0.0027 | 0.0012 | 0.002 | 0.0026 | 0.0069 | 0.0043 | 0.0003 | 0.0121 | 0.0146 | 0.0026 | 0.0075 | 0.002 | 0.0012 | 0.0014 | 0.004 |
Vacuole Periphery | 0.0011 | 0.0001 | 0.0002 | 0.0008 | 0.0009 | 0.0002 | 0.0013 | 0.0003 | 0.0003 | 0 | 0.002 | 0.0014 | 0.0006 | 0.0005 | 0.0006 | 0.0004 | 0.0006 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.9452 | 10.9266 | 10.6879 | 11.2808 | 11.5256 | 13.3268 | 12.4289 | 11.1849 | 9.1115 | 9.8362 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|