Standard name
Human Ortholog
Description Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.08 0 0.06 0 – 0 0 0 0 0.06 0.11 0.17 0.06 0.1 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.1 0 0 0 0.36 0.09 – 0.36 0.26 0.39 0.42 0 0 0 0 0.05 0 0 0 0 0 0 0
Nucleus 0.95 0.9 0.97 0.92 0.93 0.81 0.9 – 0.85 0.85 0.82 0.8 0.92 0.88 0.8 0.81 0.73 0.77 0.89 0.91 0.84 0.81 0.71 0.6
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0.08 0 0.06 0.1
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0 0 0 – 0 0 0 0 0 0 0 0.17 0.16 0.13 0 0 0 0.11 0.17 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 0 2 0 – 1 3 8 1 0 0 0 0 0 0 1 1 0 1 1 5
Bud Neck 0 0 0 0 0 0 0 – 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 4 2 – 4 5 10 12 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 7 2 1 14 10 14 11 – 10 14 15 13 12 52 79 15 14 11 1 1 0 0 2 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 1 0 1 6 6 5 0 0 0 0 2 0
Endosome 0 0 0 0 2 3 2 – 0 0 0 1 1 0 2 3 1 3 0 0 0 0 4 6
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 2
Mitochondria 1 11 2 1 2 80 29 – 103 89 162 175 0 1 2 6 7 4 1 0 1 0 2 5
Nucleus 209 98 78 153 254 180 292 – 243 289 340 337 200 410 370 194 107 90 199 101 88 158 297 246
Nuclear Periphery 0 0 0 0 5 1 3 – 4 2 1 4 0 9 11 0 0 1 1 0 0 0 1 7
Nucleolus 1 3 1 1 0 1 1 – 2 3 11 4 1 5 9 6 4 2 10 1 8 6 27 39
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 3 0 0 1 0 0 0 0 1 0
SpindlePole 0 0 0 1 0 1 5 – 0 6 1 2 0 1 2 1 0 2 0 1 0 0 3 6
Vac/Vac Membrane 5 5 0 1 7 7 5 – 6 8 7 11 2 5 9 40 23 15 6 4 3 21 69 74
Unique Cell Count 220 109 80 167 272 223 323 285 342 416 421 218 468 462 239 147 117 225 112 105 195 419 407
Labelled Cell Count 223 119 82 171 280 293 350 373 419 557 560 218 483 488 271 162 135 225 112 105 195 419 407


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 5.6 6.5 6.7 6.7 4.1 5.0 – 4.5 4.6 4.6 4.5 7.7 7.2 7.3 7.7 7.8 7.8 6.9 6.9 7.0
Std Deviation (1e-4) 1.2 1.0 1.6 1.5 1.5 1.0 0.9 – 1.0 0.9 1.2 1.0 1.6 1.9 2.0 1.8 1.9 2.1 1.4 1.5 1.8
Intensity Change (Log2) – – – 0.03 0.05 -0.67 -0.38 – -0.54 -0.52 -0.52 -0.52 0.24 0.14 0.15 0.23 0.26 0.26 0.08 0.09 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 – 0 0 0 0 0 2.8 3.7 0 2.4 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 1.9 – 0 0 0 0 0 0 0 0 0 0
Nucleus -1.8 -1.4 -3.6 -2.1 – -3.0 -3.1 -3.5 -3.8 -1.8 -2.6 -3.8 -3.6 -4.6 -4.0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 3.9 3.7 3.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0669 1.2614 1.4038 1.8295 0.4763 0.9473 2.0977 3.0643 2.6901 2.5968 2.2078 2.6463 4.0499 5.3001 4.8562 4.7188 4.0378 4.7654
Actin 0.0453 0 0.0188 0.0824 0 0.0015 0.0017 0 0 0 0 0 0.001 0 0.0033 0 0 0.0016
Bud 0.0003 0 0.0003 0.0033 0 0.0001 0 0 0 0 0 0 0.0003 0 0 0.0001 0 0.0001
Bud Neck 0.0052 0.0008 0.0025 0.003 0.0005 0.0015 0.0004 0.0001 0.0001 0.0002 0.0001 0.0009 0.0006 0.0004 0.0003 0.0153 0.001 0.0019
Bud Periphery 0.0005 0.0002 0.0007 0.0075 0.0001 0.0001 0.0001 0 0.0001 0 0 0.0001 0.0007 0 0.0001 0.0001 0.0001 0.0002
Bud Site 0.001 0.0002 0.0016 0.0046 0.0001 0.0002 0.0002 0.0001 0.0001 0 0 0.0001 0.001 0.0003 0.0003 0.0009 0.0001 0.0004
Cell Periphery 0.0002 0.0005 0.0002 0.0006 0.0001 0 0 0 0.0001 0 0 0 0.0006 0 0 0.0001 0.0001 0.0001
Cytoplasm 0.0039 0.0002 0.001 0.0031 0.0001 0.0008 0.0013 0.0003 0.0001 0.0001 0.0002 0.0001 0.0011 0.0003 0.0004 0.0003 0.0002 0.0003
Cytoplasmic Foci 0.0039 0 0.0027 0.0047 0.0001 0.0007 0.0072 0 0 0 0 0 0.0015 0.0003 0.0006 0.0016 0.0005 0.0003
Eisosomes 0.0005 0 0.0001 0.0008 0 0 0 0 0 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0025 0.0001 0.0009 0.0055 0 0.0006 0.0007 0 0 0 0 0 0.0009 0 0.0002 0 0 0.0001
Endosome 0.0078 0.0003 0.0071 0.0282 0.0001 0.004 0.0047 0 0.0001 0 0.0001 0 0.0029 0 0.0011 0.0011 0.0001 0.0012
Golgi 0.0039 0 0.0026 0.0144 0 0.0004 0.0008 0 0 0 0 0 0.0006 0 0.0003 0.0003 0 0.0007
Lipid Particles 0.0063 0.0001 0.0047 0.0111 0 0.0007 0.0021 0 0 0 0 0 0.0039 0 0.0005 0.0008 0.0001 0.0009
Mitochondria 0.0021 0.0002 0.0026 0.0114 0.0001 0.0004 0.0005 0.0001 0.0005 0.0002 0.0001 0.0001 0.0025 0.0002 0.0005 0.0003 0.0002 0.0007
None 0.0032 0 0.0013 0.006 0 0.0008 0.0028 0 0 0 0.0001 0 0.0018 0.0001 0.0006 0.0001 0.0001 0.0001
Nuclear Periphery 0.0123 0.0042 0.0052 0.0238 0.0009 0.0198 0.0058 0.0029 0.001 0.001 0.0111 0.0005 0.0035 0.0014 0.0013 0.0011 0.0017 0.0028
Nucleolus 0.0374 0.0476 0.0269 0.0256 0.051 0.0589 0.0295 0.0447 0.0448 0.0396 0.0218 0.0577 0.0254 0.065 0.07 0.1425 0.0263 0.0827
Nucleus 0.8481 0.9384 0.901 0.7432 0.9093 0.8985 0.93 0.9477 0.9501 0.9579 0.9636 0.9352 0.9225 0.9272 0.917 0.7839 0.9167 0.8958
Peroxisomes 0.0058 0 0.0018 0.0028 0 0.0001 0.0006 0 0 0 0 0 0.0017 0 0.0004 0.0001 0 0.0003
Punctate Nuclear 0.0076 0.0008 0.0155 0.0075 0.0363 0.0099 0.0111 0.0035 0.0023 0.0006 0.0025 0.0052 0.0237 0.0046 0.0029 0.0507 0.0525 0.0093
Vacuole 0.0016 0.006 0.0014 0.0062 0.0009 0.0005 0.0003 0.0002 0.0005 0.0001 0.0001 0.0001 0.0027 0.0002 0.0002 0.0007 0.0003 0.0004
Vacuole Periphery 0.0009 0.0003 0.0011 0.0045 0.0001 0.0005 0.0002 0.0001 0.0002 0.0001 0.0001 0 0.0008 0.0001 0.0001 0.0001 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.8271 11.7426 17.427 17.3648 25.5466 5.6265 13.145 12.5925 15.2378 21.4497
Translational Efficiency 1.6827 1.8667 1.3668 0.9824 0.8892 4.0143 1.4342 1.1917 1.0005 1.2016

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1698 534 862 1695 1662 2469 2551 1445 3360 3003 3413 3140

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 799.83 833.87 999.33 1071.09 796.54 899.10 1051.51 1105.72 798.20 887.50 1038.33 1087.03
Standard Deviation 132.37 153.23 134.42 177.79 109.41 126.40 145.26 167.71 121.56 133.91 144.39 174.08
Intensity Change Log 2 — 0.060129 0.321268 0.421314 — 0.174735 0.400644 0.473167 — 0.118452 0.361420 0.447420

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000229 0.000539 0.000626 0.000672 0.000223 0.000208 0.000404 0.001351 0.000226 0.000267 0.000460 0.000985
Bud Neck 0.002817 0.007604 0.025411 0.021925 0.002891 0.009899 0.023931 0.040777 0.002854 0.009491 0.024305 0.030601
Bud Site 0.000773 0.001338 0.001792 0.001957 0.000425 0.002138 0.000703 0.006674 0.000601 0.001995 0.000979 0.004128
Cell Periphery 0.000143 0.000153 0.000093 0.000105 0.000108 0.000174 0.000060 0.000149 0.000126 0.000170 0.000068 0.000125
Cytoplasm 0.007777 0.006831 0.001147 0.004054 0.003099 0.003314 0.000428 0.011558 0.005463 0.003940 0.000609 0.007508
Cytoplasmic Foci 0.001502 0.001677 0.000033 0.001021 0.000332 0.000351 0.000006 0.003343 0.000923 0.000587 0.000013 0.002090
Eisosomes 0.000016 0.000023 0.000032 0.000023 0.000019 0.000020 0.000033 0.000033 0.000018 0.000020 0.000033 0.000027
Endoplasmic Reticulum 0.005919 0.008089 0.005036 0.004998 0.004873 0.006627 0.003506 0.006738 0.005402 0.006887 0.003892 0.005799
Endosome 0.000697 0.001159 0.000232 0.000885 0.000304 0.001010 0.000121 0.007583 0.000503 0.001037 0.000149 0.003967
Golgi 0.000393 0.000515 0.000062 0.001155 0.000341 0.000211 0.000034 0.004668 0.000367 0.000265 0.000041 0.002772
Lipid Particles 0.000466 0.000869 0.000078 0.000070 0.000350 0.000179 0.000017 0.000301 0.000409 0.000302 0.000033 0.000176
Mitochondria 0.000762 0.002101 0.001145 0.002916 0.000982 0.002756 0.000729 0.006155 0.000871 0.002639 0.000834 0.004407
Mitotic Spindle 0.000956 0.003572 0.000705 0.005067 0.000541 0.002471 0.000346 0.021454 0.000751 0.002666 0.000437 0.012608
None 0.010713 0.009045 0.001907 0.004361 0.003489 0.003864 0.000913 0.004196 0.007139 0.004785 0.001164 0.004285
Nuclear Periphery 0.000703 0.001108 0.000448 0.001282 0.000345 0.001144 0.000296 0.005246 0.000526 0.001138 0.000334 0.003106
Nuclear Periphery Foci 0.000487 0.002391 0.000151 0.000455 0.000317 0.000184 0.000042 0.002607 0.000403 0.000576 0.000070 0.001445
Nucleolus 0.020666 0.028005 0.018559 0.008762 0.020671 0.013277 0.019496 0.011116 0.020668 0.015896 0.019259 0.009845
Nucleus 0.943549* 0.921265* 0.941236* 0.934678* 0.959557* 0.948672* 0.948319* 0.848061* 0.951467* 0.943798* 0.946530* 0.894818*
Peroxisomes 0.000341 0.000887 0.000244 0.000131 0.000414 0.000518 0.000110 0.000614 0.000377 0.000584 0.000144 0.000353
Vacuole 0.000860 0.002260 0.000917 0.003934 0.000541 0.002317 0.000381 0.012740 0.000702 0.002307 0.000517 0.007986
Vacuole Periphery 0.000230 0.000568 0.000144 0.001547 0.000180 0.000667 0.000123 0.004634 0.000205 0.000650 0.000128 0.002967

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.44 -8.44 -9.44 -2.94 -0.89 0.44 -7.03 -7.70 -7.83 -6.56 -1.78 -11.97 -10.74 -10.07 -7.44
Bud Neck -4.06 -12.95 -18.96 -11.14 0.51 -7.53 -25.76 -22.69 -18.52 -11.99 -8.49 -28.66 -28.75 -20.57 -8.92
Bud Site -1.45 -2.22 -3.47 -1.68 -0.37 -4.04 -2.42 -8.73 -5.80 -8.26 -3.76 -2.30 -9.29 -4.74 -8.12
Cell Periphery -0.26 2.14 2.36 1.92 -0.19 -2.17 2.71 -0.99 1.72 -4.88 -1.74 4.00 1.25 2.65 -3.88
Cytoplasm 0.24 5.22 2.11 1.35 -4.48 -0.38 3.92 -5.15 -5.12 -7.68 1.52 6.71 -1.99 -3.72 -9.16
Cytoplasmic Foci -0.61 3.23 1.37 1.59 -1.83 0.09 3.18 -2.72 -2.78 -3.54 1.06 3.89 -0.51 -1.50 -3.86
Eisosomes -4.26 -10.19 -6.35 0.48 6.16 -0.18 -7.21 -7.88 -8.64 -1.88 -2.17 -11.85 -9.79 -7.25 2.56
Endoplasmic Reticulum -3.17 1.26 1.71 4.50 0.33 -4.01 4.68 -6.20 -1.80 -12.25 -4.01 5.76 -2.67 1.89 -9.68
Endosome -1.39 2.54 -0.05 1.41 -3.18 -2.91 3.68 -4.61 -3.64 -4.85 -2.38 3.76 -4.43 -2.84 -5.49
Golgi -0.42 1.48 -0.89 -0.64 -1.66 0.95 2.15 -3.21 -3.56 -3.86 0.78 2.52 -2.92 -3.36 -3.99
Lipid Particles -0.76 2.74 2.90 1.36 0.55 0.86 1.66 -0.19 -4.00 -9.64 0.53 3.13 1.57 0.78 -9.19
Mitochondria -1.18 -2.83 -4.61 -0.64 -3.35 -2.27 0.44 -4.94 -2.47 -6.32 -2.93 -0.14 -6.53 -1.80 -7.49
Mitotic Spindle -1.32 -0.25 -3.17 -0.65 -3.19 -2.50 0.71 -7.99 -7.22 -8.07 -2.55 0.56 -8.53 -6.86 -8.77
None 0.67 5.71 4.08 2.50 -2.68 -0.48 5.10 -1.24 -0.60 -6.10 2.28 7.36 3.25 0.81 -6.37
Nuclear Periphery -1.80 1.04 -4.64 -1.75 -5.14 -5.42 0.48 -6.33 -5.12 -6.37 -4.67 2.48 -7.50 -5.44 -7.95
Nuclear Periphery Foci -1.15 0.75 -0.22 1.12 -0.82 1.49 3.65 -4.27 -4.65 -5.04 -0.54 3.47 -3.87 -1.91 -5.30
Nucleolus -2.45 0.14 6.18 5.90 4.41 4.67 0.30 0.88 -3.46 0.66 3.80 0.33 4.82 1.19 4.37
Nucleus 3.24 1.66 3.49 -1.02 1.83 3.02 4.99 19.37 17.46 17.87 2.45 3.36 16.98 14.17 15.90
Peroxisomes -2.00 0.75 2.01 2.98 3.12 -0.32 3.15 -0.37 0.10 -3.17 -0.89 3.17 1.07 1.39 -2.58
Vacuole -1.77 -1.19 -4.48 -1.48 -4.19 -4.60 1.53 -9.72 -8.47 -9.82 -4.54 1.21 -10.58 -8.15 -10.75
Vacuole Periphery -1.88 1.99 -2.96 -1.80 -3.20 -4.10 1.71 -4.22 -3.26 -4.33 -4.37 2.87 -5.15 -3.58 -5.41
Standard name
Human Ortholog
Description Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Pop3

Pop3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pop3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available