Standard name | |
---|---|
Human Ortholog | |
Description | Sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway; involved in maintenance of cell wall integrity and recovery from heat shock; required for the arrest of secretion response; WSC2 has a paralog, WSC3, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 105 | 122 | 113 | 56 | 39 | 78 | 86 | 80 | – | 41 | 59 | 50 | 16 | 15 | 21 | 71 | 95 | 100 | 35 | 81 | 43 | 26 | 24 | 17 |
Bud | 25 | 46 | 27 | 25 | 19 | 46 | 49 | 56 | – | 40 | 71 | 46 | 10 | 6 | 6 | 18 | 21 | 14 | 3 | 13 | 4 | 13 | 13 | 10 |
Bud Neck | 0 | 1 | 11 | 4 | 2 | 5 | 3 | 3 | – | 0 | 3 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 21 | 85 | 33 | 18 | 12 | 12 |
Bud Site | 12 | 14 | 12 | 16 | 4 | 7 | 9 | 8 | – | 6 | 2 | 10 | 0 | 0 | 1 | 1 | 1 | 3 | – | – | – | – | – | – |
Cell Periphery | 90 | 155 | 112 | 30 | 31 | 45 | 71 | 34 | – | 30 | 55 | 69 | 39 | 46 | 90 | 183 | 153 | 151 | 190 | 216 | 243 | 65 | 118 | 151 |
Cytoplasm | 51 | 147 | 102 | 62 | 84 | 160 | 186 | 212 | – | 205 | 236 | 267 | 24 | 22 | 51 | 104 | 119 | 122 | 3 | 22 | 2 | 0 | 0 | 3 |
Endoplasmic Reticulum | 7 | 13 | 5 | 3 | 4 | 18 | 15 | 10 | – | 9 | 9 | 10 | 1 | 1 | 4 | 29 | 16 | 13 | 28 | 28 | 21 | 7 | 4 | 8 |
Endosome | 9 | 0 | 2 | 3 | 4 | 1 | 0 | 3 | – | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 1 | 3 | 4 | 0 | 0 | 0 | 0 |
Golgi | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 0 |
Mitochondria | 7 | 9 | 17 | 14 | 15 | 45 | 61 | 56 | – | 54 | 93 | 79 | 0 | 0 | 1 | 42 | 19 | 20 | 7 | 12 | 5 | 2 | 3 | 3 |
Nucleus | 2 | 1 | 0 | 1 | 1 | 0 | 2 | 1 | – | 1 | 0 | 1 | 0 | 0 | 1 | 3 | 2 | 8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 7 | 0 | 8 | 7 | 21 | 5 | 6 | 5 | – | 3 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 18 | 3 | 30 | 14 | 18 | 1 | 8 | 7 | – | 4 | 4 | 7 | 5 | 3 | 2 | 10 | 15 | 29 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 0 | 3 | 0 | 3 | 0 | 0 | 1 | – | 0 | 3 | 1 | 0 | 0 | 0 | 3 | 3 | 2 | 12 | 24 | 7 | 1 | 2 | 1 |
Unique Cell Count | 219 | 369 | 304 | 166 | 179 | 276 | 360 | 350 | 301 | 388 | 389 | 62 | 70 | 135 | 327 | 334 | 351 | 312 | 495 | 373 | 138 | 184 | 215 | |
Labelled Cell Count | 339 | 512 | 443 | 235 | 245 | 411 | 496 | 476 | 393 | 537 | 544 | 95 | 93 | 177 | 468 | 448 | 463 | 312 | 495 | 373 | 138 | 184 | 215 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.7 | 6.6 | 5.8 | 5.1 | 5.8 | 4.7 | 4.7 | 5.0 | – | 4.4 | 4.3 | 4.5 | 7.9 | 8.1 | 8.6 | 7.6 | 8.4 | 9.1 | 9.3 | 10.4 | 11.7 |
Std Deviation (1e-4) | 1.2 | 1.2 | 1.3 | 1.3 | 2.1 | 1.3 | 1.0 | 0.9 | – | 1.0 | 1.0 | 0.8 | 1.4 | 1.5 | 1.9 | 1.2 | 1.5 | 1.6 | 1.7 | 1.9 | 2.2 |
Intensity Change (Log2) | – | – | – | -0.19 | -0.0 | -0.31 | -0.3 | -0.2 | – | -0.41 | -0.42 | -0.35 | 0.45 | 0.48 | 0.57 | 0.4 | 0.54 | 0.65 | 0.68 | 0.84 | 1.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.7 | -3.5 | 0 | 0 | -4.0 | – | 0 | 0 | 0 | -1.7 | -2.5 | -4.5 | -4.3 | -2.3 | -2.4 |
Bud | 2.0 | 0.6 | 0 | 0 | 2.7 | – | 0 | 0 | 0 | 1.7 | -0.1 | -1.6 | -1.6 | -1.2 | -2.6 |
Bud Neck | -0.7 | 0 | 0 | 0 | -2.4 | – | 0 | 0 | 0 | 0 | 0 | 0 | -3.0 | -2.7 | -3.6 |
Bud Site | 2.5 | -1.0 | 0 | 0 | -1.2 | – | 0 | 0 | 0 | 0 | 0 | 0 | -3.2 | -3.3 | -2.6 |
Cell Periphery | -4.2 | -4.5 | 0 | 0 | -8.3 | – | 0 | 0 | 0 | 3.8 | 4.4 | 5.8 | 4.8 | 2.3 | 1.6 |
Cytoplasm | 0.8 | 2.9 | 5.9 | 4.7 | 6.9 | – | 8.5 | 7.1 | 9.2 | 0.8 | -0.3 | 0.9 | -0.5 | 0.6 | 0.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 1.0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 4.0 | 2.2 | 1.6 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.2 | 1.2 | 0 | 0 | 4.2 | – | 0 | 0 | 0 | 0 | 0 | -2.4 | 3.1 | 0.1 | 0.1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0.9 | 4.1 | 0 | 0 | -1.1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -0.5 | 0.1 | 0 | 0 | -4.3 | – | 0 | 0 | 0 | -0.4 | -1.5 | -3.1 | -3.5 | -2.6 | -0.7 |
Vacuole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.4425 | 5.1796 | 5.2677 | 4.8286 | 4.6272 | 4.7219 | 5.8874 | 6.4472 | 6.5189 | 6.5125 | 6.5872 | 6.568 | 5.9103 | 5.783 | 5.8827 | 6.2217 | 6.19 | 6.315 |
Actin | 0.0107 | 0.0157 | 0.0182 | 0.0035 | 0.0044 | 0.0041 | 0.0125 | 0.0098 | 0.0087 | 0.0033 | 0.0035 | 0.0045 | 0.0135 | 0.0235 | 0.0235 | 0.0041 | 0.0282 | 0.0068 |
Bud | 0.0072 | 0.0089 | 0.0199 | 0.0094 | 0.005 | 0.0034 | 0.0031 | 0.0034 | 0.0101 | 0.0037 | 0.0012 | 0.0025 | 0.0018 | 0.0027 | 0.0141 | 0.0039 | 0.0018 | 0.002 |
Bud Neck | 0.0074 | 0.0078 | 0.0274 | 0.0315 | 0.01 | 0.077 | 0.0083 | 0.0105 | 0.0504 | 0.0751 | 0.058 | 0.0903 | 0.0041 | 0.0125 | 0.0299 | 0.0496 | 0.0158 | 0.0792 |
Bud Periphery | 0.0666 | 0.0406 | 0.1619 | 0.1673 | 0.1013 | 0.051 | 0.042 | 0.0194 | 0.1129 | 0.1051 | 0.0298 | 0.0339 | 0.025 | 0.0185 | 0.1283 | 0.0877 | 0.0445 | 0.0354 |
Bud Site | 0.0329 | 0.2329 | 0.0979 | 0.0174 | 0.0084 | 0.0114 | 0.0428 | 0.3332 | 0.1149 | 0.0135 | 0.0189 | 0.0099 | 0.0267 | 0.2981 | 0.0996 | 0.0105 | 0.0028 | 0.0069 |
Cell Periphery | 0.547 | 0.2831 | 0.4272 | 0.4315 | 0.581 | 0.5226 | 0.6774 | 0.4391 | 0.5538 | 0.6458 | 0.731 | 0.6666 | 0.7287 | 0.387 | 0.5625 | 0.6865 | 0.6872 | 0.6627 |
Cytoplasm | 0.2208 | 0.2863 | 0.163 | 0.2151 | 0.185 | 0.2068 | 0.1682 | 0.1517 | 0.1204 | 0.1202 | 0.137 | 0.1671 | 0.1543 | 0.2243 | 0.1188 | 0.1375 | 0.1942 | 0.1846 |
Cytoplasmic Foci | 0.0028 | 0.0044 | 0.0031 | 0.0047 | 0.0012 | 0.0026 | 0.0008 | 0.0006 | 0.0009 | 0.0003 | 0.0005 | 0.0004 | 0.0013 | 0.0005 | 0.0005 | 0.0003 | 0.0005 | 0.0003 |
Eisosomes | 0.0049 | 0.0039 | 0.0043 | 0.0007 | 0.0017 | 0.0011 | 0.0095 | 0.0038 | 0.0021 | 0.0022 | 0.0013 | 0.0014 | 0.0036 | 0.0064 | 0.0039 | 0.0005 | 0.0051 | 0.0011 |
Endoplasmic Reticulum | 0.0127 | 0.01 | 0.0061 | 0.0116 | 0.0072 | 0.0056 | 0.0068 | 0.0068 | 0.004 | 0.0164 | 0.005 | 0.0045 | 0.0075 | 0.0043 | 0.0067 | 0.0048 | 0.0034 | 0.0024 |
Endosome | 0.0124 | 0.0129 | 0.009 | 0.0131 | 0.0036 | 0.0067 | 0.0003 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0001 |
Golgi | 0.0012 | 0.0019 | 0.0009 | 0.0011 | 0.0004 | 0.0005 | 0.0006 | 0.0003 | 0.0006 | 0.0004 | 0.0003 | 0.0002 | 0.001 | 0.0005 | 0.0005 | 0.0002 | 0.0006 | 0.0002 |
Lipid Particles | 0.003 | 0.003 | 0.0008 | 0.0011 | 0.0013 | 0.0023 | 0.0023 | 0.0012 | 0.0013 | 0.0029 | 0.0008 | 0.0012 | 0.0015 | 0.0009 | 0.0007 | 0.0004 | 0.0007 | 0.0005 |
Mitochondria | 0.0008 | 0.0052 | 0.0008 | 0.0071 | 0.0003 | 0.0004 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0.0038 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 |
None | 0.0265 | 0.0261 | 0.0155 | 0.0394 | 0.0265 | 0.0187 | 0.0156 | 0.0123 | 0.0082 | 0.0006 | 0.0064 | 0.0092 | 0.0185 | 0.0139 | 0.0014 | 0.0008 | 0.0111 | 0.0104 |
Nuclear Periphery | 0.0008 | 0.0014 | 0.0007 | 0.001 | 0.0005 | 0.0005 | 0.0005 | 0.0005 | 0.0004 | 0.0007 | 0.0002 | 0.0002 | 0.0005 | 0.0005 | 0.0004 | 0.0003 | 0.0002 | 0.0001 |
Nucleolus | 0.0005 | 0.0013 | 0.0006 | 0.0008 | 0.0007 | 0.0008 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Nucleus | 0.0008 | 0.0032 | 0.0016 | 0.0026 | 0.0022 | 0.0021 | 0.0004 | 0.0007 | 0.0007 | 0.0005 | 0.0005 | 0.0006 | 0.0002 | 0.0005 | 0.0006 | 0.0005 | 0.0002 | 0.0004 |
Peroxisomes | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0012 | 0 | 0 | 0 | 0.0008 | 0 | 0.0003 | 0 | 0 | 0 |
Punctate Nuclear | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 |
Vacuole | 0.0392 | 0.0489 | 0.0402 | 0.0379 | 0.0585 | 0.08 | 0.0082 | 0.0061 | 0.0076 | 0.0084 | 0.0049 | 0.0068 | 0.0063 | 0.0053 | 0.0073 | 0.0116 | 0.0035 | 0.0066 |
Vacuole Periphery | 0.0015 | 0.0015 | 0.0007 | 0.0029 | 0.0006 | 0.0018 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 93.045 | 71.1078 | 28.0768 | 26.2907 | 62.9204 | 51.7846 | 60.8486 | 44.7415 | 28.6996 | 77.6347 |
Translational Efficiency | 0.5141 | 0.4177 | 0.2882 | 0.3556 | 0.5917 | 0.8027 | 0.3783 | 0.3608 | 0.4015 | 0.39 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
205 | 976 | 2673 | 481 | 300 | 1499 | 1031 | 1523 | 505 | 2475 | 3704 | 2004 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 933.37 | 868.35 | 955.30 | 1102.08 | 768.57 | 823.16 | 919.96 | 962.03 | 835.47 | 840.98 | 945.46 | 995.64 |
Standard Deviation | 135.54 | 111.15 | 108.03 | 153.03 | 81.03 | 107.02 | 136.45 | 137.24 | 133.82 | 110.89 | 117.71 | 153.34 |
Intensity Change Log 2 | — | -0.104172 | 0.033505 | 0.239708 | — | 0.098996 | 0.259394 | 0.323905 | — | -0.008868 | 0.139910 | 0.278340 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.033234 | 0.026133 | 0.035363 | 0.029310 | 0.009555 | 0.018783 | 0.045064 | 0.036218 | 0.019168 | 0.021681 | 0.038063 | 0.034560 |
Bud Neck | 0.065272 | 0.108182 | 0.169569 | 0.038602 | 0.172729 | 0.150211 | 0.098778 | 0.064634 | 0.129108 | 0.133637 | 0.149865 | 0.058385 |
Bud Site | 0.217371* | 0.085022 | 0.192734* | 0.156991* | 0.061400 | 0.105336* | 0.139710* | 0.166081* | 0.124715* | 0.097325 | 0.177975* | 0.163899* |
Cell Periphery | 0.145165* | 0.287881* | 0.170854* | 0.559432* | 0.189355* | 0.158730* | 0.319347* | 0.354552* | 0.171416* | 0.209660* | 0.212186* | 0.403727* |
Cytoplasm | 0.215614* | 0.369861* | 0.285075* | 0.131514* | 0.411217* | 0.418490* | 0.190929* | 0.274713* | 0.331814* | 0.399314* | 0.258869* | 0.240342* |
Cytoplasmic Foci | 0.026951 | 0.013996 | 0.010290 | 0.000930 | 0.021040 | 0.023275 | 0.010219 | 0.011457 | 0.023439 | 0.019616 | 0.010271 | 0.008930 |
Eisosomes | 0.015944 | 0.047720 | 0.029160 | 0.022491 | 0.055192 | 0.039755 | 0.048127 | 0.021948 | 0.039260 | 0.042896 | 0.034440 | 0.022079 |
Endoplasmic Reticulum | 0.006720 | 0.005550 | 0.014461 | 0.006626 | 0.002452 | 0.004209 | 0.024522 | 0.003986 | 0.004185 | 0.004738 | 0.017262 | 0.004619 |
Endosome | 0.013092 | 0.003187 | 0.001559 | 0.000234 | 0.004926 | 0.004875 | 0.001314 | 0.003244 | 0.008241 | 0.004209 | 0.001491 | 0.002522 |
Golgi | 0.011786 | 0.007202 | 0.001295 | 0.000244 | 0.008457 | 0.008584 | 0.002398 | 0.003080 | 0.009808 | 0.008039 | 0.001602 | 0.002399 |
Lipid Particles | 0.001729 | 0.004066 | 0.003031 | 0.001309 | 0.011628 | 0.004762 | 0.002964 | 0.002291 | 0.007610 | 0.004487 | 0.003012 | 0.002055 |
Mitochondria | 0.015721 | 0.002622 | 0.001371 | 0.000238 | 0.007846 | 0.009539 | 0.000418 | 0.001077 | 0.011043 | 0.006811 | 0.001106 | 0.000875 |
Mitotic Spindle | 0.004602 | 0.000691 | 0.000216 | 0.000203 | 0.000139 | 0.000316 | 0.000234 | 0.001150 | 0.001951 | 0.000464 | 0.000221 | 0.000922 |
None | 0.000054 | 0.000110 | 0.000439 | 0.000032 | 0.000176 | 0.000758 | 0.000445 | 0.000514 | 0.000126 | 0.000503 | 0.000441 | 0.000398 |
Nuclear Periphery | 0.000367 | 0.000084 | 0.000106 | 0.000096 | 0.000120 | 0.000081 | 0.000178 | 0.000060 | 0.000220 | 0.000082 | 0.000126 | 0.000068 |
Nuclear Periphery Foci | 0.000930 | 0.000880 | 0.000606 | 0.000568 | 0.000308 | 0.000236 | 0.000966 | 0.000723 | 0.000561 | 0.000490 | 0.000706 | 0.000686 |
Nucleolus | 0.000045 | 0.000109 | 0.000080 | 0.000012 | 0.000205 | 0.000448 | 0.000069 | 0.000066 | 0.000140 | 0.000314 | 0.000077 | 0.000053 |
Nucleus | 0.015930 | 0.007346 | 0.034280 | 0.004446 | 0.013212 | 0.013330 | 0.068040 | 0.008941 | 0.014315 | 0.010970 | 0.043677 | 0.007862 |
Peroxisomes | 0.001305 | 0.005840 | 0.002175 | 0.000394 | 0.004642 | 0.006694 | 0.001578 | 0.001614 | 0.003288 | 0.006357 | 0.002009 | 0.001322 |
Vacuole | 0.199264 | 0.022564 | 0.047144 | 0.046234 | 0.024962 | 0.030083 | 0.044601 | 0.043387 | 0.095718 | 0.027118 | 0.046436 | 0.044070 |
Vacuole Periphery | 0.008904 | 0.000954 | 0.000189 | 0.000093 | 0.000438 | 0.001504 | 0.000100 | 0.000267 | 0.003875 | 0.001287 | 0.000165 | 0.000225 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.00 | -1.07 | 0.17 | -1.04 | 1.69 | -3.74 | -9.71 | -9.76 | -7.29 | 2.04 | -1.48 | -7.90 | -6.30 | -6.53 | 1.38 |
Bud Neck | -4.59 | -10.83 | 2.15 | 10.89 | 23.42* | 1.68 | 5.91 | 8.52 | 14.91 | 5.29 | -0.75 | -2.33 | 7.69 | 17.23 | 21.88 |
Bud Site | 6.03 | 0.42 | 2.06 | -6.58 | 3.24 | -5.38 | -8.04 | -11.46 | -8.24 | -3.85 | 2.12 | -5.95 | -4.39 | -11.07 | 1.99 |
Cell Periphery | -8.37 | -2.28 | -18.69 | -14.62 | -23.65 | 2.14 | -7.22 | -10.37 | -18.17 | -3.82 | -3.82 | -3.77 | -18.09 | -20.01 | -21.45 |
Cytoplasm | -8.65 | -4.23 | 4.64 | 21.65 | 16.96 | -0.45 | 12.87 | 8.08 | 15.27 | -8.68 | -5.19 | 5.56 | 6.83 | 21.10 | 2.94 |
Cytoplasmic Foci | 3.15 | 4.04 | 6.18 | 9.18 | 11.99 | -0.57 | 3.16 | 2.84 | 5.74 | -0.73 | 1.63 | 5.14 | 5.51 | 7.28 | 1.22 |
Eisosomes | -9.84 | -5.80 | -2.37 | 7.34 | 2.57 | 2.83 | 1.62 | 6.27 | 8.23 | 8.57 | -1.17 | 1.41 | 4.77 | 11.40 | 7.72 |
Endoplasmic Reticulum | 0.41 | -3.78 | -0.11 | -0.68 | 4.67 | -2.15 | -10.65 | -2.15 | 0.27 | 10.02 | -0.69 | -10.71 | -0.60 | 0.14 | 12.53 |
Endosome | 3.12 | 3.69 | 4.13 | 8.26 | 11.82 | 0.07 | 4.28 | 1.77 | 2.66 | -3.86 | 2.84 | 4.97 | 4.04 | 3.73 | -2.87 |
Golgi | 0.95 | 2.59 | 2.88 | 12.01 | 9.57 | -0.06 | 4.83 | 3.85 | 5.02 | -1.12 | 0.77 | 4.73 | 4.06 | 7.39 | -1.64 |
Lipid Particles | -2.82 | -2.65 | 1.29 | 3.86 | 5.69 | 1.95 | 2.44 | 2.61 | 4.04 | 1.11 | 1.51 | 2.21 | 2.61 | 4.86 | 2.46 |
Mitochondria | 2.04 | 2.27 | 2.47 | 4.00 | 10.31 | -0.48 | 2.29 | 2.09 | 6.92 | -2.80 | 1.18 | 3.11 | 3.18 | 7.54 | 1.21 |
Mitotic Spindle | 1.30 | 1.50 | 1.51 | 1.08 | 0.29 | -1.59 | -1.42 | -1.51 | -1.26 | -1.18 | 1.21 | 1.42 | 0.75 | -0.84 | -1.32 |
None | -2.01 | -4.23 | 2.17 | 4.74 | 4.68 | -1.55 | -1.97 | -1.05 | 0.39 | -0.22 | -1.64 | -3.36 | -1.12 | 0.18 | 0.04 |
Nuclear Periphery | 1.15 | 1.06 | 1.10 | -0.76 | 1.26 | 1.71 | -3.96 | 2.87 | 2.21 | 9.06 | 1.37 | 0.78 | 1.52 | 1.63 | 10.82 |
Nuclear Periphery Foci | 0.37 | 2.35 | 2.41 | 0.65 | 0.39 | 1.62 | -9.14 | -1.23 | -1.43 | 0.43 | 0.64 | -0.86 | -0.39 | -0.72 | -0.14 |
Nucleolus | -0.89 | -2.80 | 3.48 | 1.43 | 23.07* | -0.47 | 0.83 | 0.92 | 1.05 | 0.48 | -0.58 | 0.76 | 0.96 | 1.18 | 1.24 |
Nucleus | 2.71 | -4.03 | 3.32 | 2.57 | 18.75 | -0.04 | -11.71 | 2.86 | 3.93 | 12.58 | 2.29 | -12.68 | 3.88 | 3.69 | 19.73 |
Peroxisomes | -6.53 | -2.29 | 2.40 | 8.95 | 9.22 | -1.85 | 3.45 | 3.43 | 7.93 | -0.32 | -4.32 | 2.30 | 3.51 | 10.90 | 3.38 |
Vacuole | 10.65 | 9.40 | 8.79 | -4.90 | -0.83 | -1.84 | -7.40 | -5.90 | -5.06 | 2.12 | 8.81 | 6.48 | 6.64 | -7.54 | 0.95 |
Vacuole Periphery | 2.28 | 2.58 | 2.61 | 1.37 | 3.19 | -2.10 | 3.09 | 1.47 | 2.48 | -3.56 | 1.72 | 2.69 | 2.64 | 2.72 | -1.50 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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