Standard name
Human Ortholog
Description Putative checkpoint factor in transcription; binds to U2 snRNA and Prp11p; regulates toggling of the U2 snRNA stem II region between different structures; contains two RNA recognition motifs (RRMs)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.08 0.09 0.08 0.13 0.08 0.07 0 0.06 0.05 0.11 0.17 0.15 0.14 0.14 0.13 0 0 0 0.05 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.15 0 0.05 0.16 0.18 0.55 0.13 0.53 0.31 0.46 0.42 0 0 0 0.06 0 0 0 0 0 0 0 0
Nucleus 0.9 0.92 0.93 0.88 0.85 0.8 0.68 0.84 0.77 0.79 0.79 0.77 0.87 0.79 0.81 0.75 0.75 0.8 0.87 0.88 0.85 0.65 0.74 0.58
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.11 0.11 0.08 0.06 0 0.07 0.1 0.1 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 0 2 0
Bud 0 0 0 1 3 1 10 1 7 5 6 6 0 0 0 0 1 2 4 0 5 1 1 3
Bud Neck 0 0 1 0 0 0 2 2 0 3 0 1 0 0 0 0 0 0 0 1 0 1 0 0
Bud Site 0 0 0 0 2 2 17 3 17 7 11 9 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 12 2 19 24 32 24 48 40 31 26 19 21 8 26 27 45 29 30 4 1 3 7 7 8
Endoplasmic Reticulum 0 0 3 0 0 0 0 0 0 1 0 0 0 0 0 15 10 8 0 0 1 2 1 1
Endosome 1 0 3 1 0 3 0 2 0 1 0 1 0 1 2 9 3 2 3 1 2 3 3 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 3 1 1 0
Mitochondria 12 24 4 14 57 57 206 63 245 183 158 175 2 1 0 18 4 3 2 4 3 1 2 8
Nucleus 266 152 386 261 297 254 255 406 361 466 271 324 61 123 145 238 159 181 271 153 373 86 184 131
Nuclear Periphery 0 0 0 2 0 4 1 3 0 6 1 6 0 0 0 5 0 1 0 0 1 0 2 0
Nucleolus 1 1 0 0 0 2 3 1 2 7 11 7 0 1 0 0 0 0 0 0 0 5 7 15
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 2 1 2 4 6 2 10 3 6 0 1 0 1 0 0 3 1 6 3 3 4
Vac/Vac Membrane 11 6 7 8 10 16 2 8 2 12 5 14 2 4 6 35 23 17 18 7 31 12 26 45
Unique Cell Count 294 165 417 297 351 316 375 486 466 588 343 421 70 155 178 319 211 226 314 174 442 132 250 226
Labelled Cell Count 304 185 424 313 403 365 548 536 667 727 485 570 73 157 181 366 230 247 314 174 442 132 250 226


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 4.5 6.3 5.9 5.1 5.1 3.4 5.3 4.2 4.6 4.2 4.5 6.2 6.3 5.8 5.9 7.3 7.1 7.0 7.2 7.3
Std Deviation (1e-4) 0.9 0.8 1.5 1.6 2.0 1.7 0.9 1.4 1.3 0.9 1.0 1.1 1.4 1.6 1.4 1.4 2.1 1.8 2.4 2.1 2.3
Intensity Change (Log2) – – – -0.08 -0.3 -0.29 -0.86 -0.24 -0.59 -0.46 -0.57 -0.48 -0.02 0.01 -0.12 -0.09 0.23 0.18 0.16 0.2 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 5.4 5.1 3.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.7134 2.4901 2.1476 1.8155 1.7516 2.0702 4.3879 5.3573 4.7011 4.4922 4.2854 4.855 2.2213 2.614 2.4334 2.8474 2.1644 2.6838
Actin 0.0215 0 0.0055 0.0006 0.001 0.0001 0.0044 0 0.0148 0.0001 0.0036 0.0001 0.0138 0.0009 0.0005 0 0.0049 0.0018
Bud 0.0002 0 0.0001 0 0.0002 0 0.0005 0 0.0003 0 0 0 0.0003 0.0001 0.0001 0 0.0002 0.0001
Bud Neck 0.0003 0.0005 0.0001 0.0001 0.0554 0.0022 0.0082 0.0001 0.0006 0.0004 0.0003 0.0015 0.0051 0.0001 0.0004 0.0001 0.0022 0.0013
Bud Periphery 0.0004 0 0.0001 0 0.0002 0 0.0019 0 0.0007 0 0 0 0.0009 0.0001 0.0003 0 0.0006 0.0004
Bud Site 0.0013 0.0008 0.0004 0 0.0021 0.0001 0.0009 0.0001 0.0008 0.0001 0.0001 0.0001 0.0031 0.0003 0.0003 0 0.0005 0.0001
Cell Periphery 0.0001 0.0001 0 0 0.0001 0 0.0002 0 0.0002 0 0 0 0.0002 0 0.0001 0 0.0001 0
Cytoplasm 0.0041 0.0048 0.0074 0.0136 0.0036 0.0028 0.0065 0.0037 0.009 0.0056 0.0058 0.015 0.0089 0.0091 0.0047 0.0019 0.0134 0.0016
Cytoplasmic Foci 0.0185 0.0001 0.0064 0.0002 0.0055 0.0001 0.0134 0 0.0006 0.0001 0.0025 0.0009 0.007 0.0017 0.0003 0 0.0061 0.0001
Eisosomes 0.0007 0 0.0001 0.0001 0 0 0.0001 0 0.0003 0.0001 0.0011 0 0.0002 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0017 0.0005 0.0015 0.0012 0.0005 0.0001 0.0021 0.0003 0.0032 0.0001 0.0004 0.0003 0.0014 0.0006 0.0008 0.0001 0.0032 0.0006
Endosome 0.0178 0.0003 0.0045 0.002 0.0046 0.0002 0.007 0.0002 0.0054 0.0001 0.0007 0.0005 0.0105 0.0018 0.0015 0.0001 0.0279 0.001
Golgi 0.0041 0 0.0023 0 0.0018 0 0.0011 0 0.0051 0 0.0001 0 0.0012 0.0004 0.0002 0 0.004 0.0005
Lipid Particles 0.0131 0.0001 0.0034 0.0002 0.0014 0 0.0058 0 0.0013 0 0.0013 0.0001 0.0028 0.0018 0.0015 0 0.0148 0.0002
Mitochondria 0.0348 0.0002 0.0102 0.0011 0.0002 0.0001 0.0029 0.0001 0.0043 0.0001 0.0004 0.0002 0.0114 0.0007 0.0028 0.0001 0.0095 0.0018
None 0.0029 0.0204 0.0181 0.0661 0.0005 0.0103 0.0044 0.0018 0.062 0.0763 0.1332 0.0721 0.0382 0.0367 0.0396 0.0005 0.0096 0.0028
Nuclear Periphery 0.0116 0.0032 0.0168 0.0364 0.004 0.0021 0.0231 0.0028 0.0145 0.0056 0.0086 0.0056 0.0098 0.0185 0.0096 0.0043 0.0387 0.0057
Nucleolus 0.005 0.0061 0.0034 0.0028 0.0121 0.0145 0.0081 0.0043 0.0059 0.0042 0.0072 0.0111 0.0062 0.012 0.005 0.0024 0.0161 0.0136
Nucleus 0.8325 0.96 0.9045 0.8656 0.8791 0.9554 0.8683 0.9837 0.8592 0.901 0.8274 0.8654 0.8494 0.8889 0.9209 0.9876 0.807 0.9551
Peroxisomes 0.015 0 0.0052 0.0001 0.0005 0 0.0011 0 0.0002 0 0.0007 0 0.0113 0.0051 0.0001 0 0.0058 0
Punctate Nuclear 0.0118 0.0014 0.0092 0.0071 0.0263 0.0115 0.0351 0.0022 0.0098 0.0058 0.0059 0.0265 0.0164 0.0199 0.0095 0.0027 0.0276 0.0123
Vacuole 0.0017 0.0014 0.0006 0.0015 0.0009 0.0002 0.003 0.0006 0.0011 0.0003 0.0005 0.0003 0.0013 0.0007 0.0012 0.0001 0.0046 0.0004
Vacuole Periphery 0.0009 0.0001 0.0003 0.001 0.0001 0 0.0018 0 0.0006 0.0001 0.0002 0.0001 0.0007 0.0005 0.0006 0 0.0033 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.7161 9.197 5.9715 10.745 11.6717 11.5678 9.8802 10.1989 12.6951 9.7087
Translational Efficiency 1.3188 1.5497 2.1037 1.4282 1.2039 0.9427 1.2491 1.3851 1.1587 1.2023

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
286 759 203 2125 2417 2423 2036 1643 2703 3182 2239 3768

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 715.61 789.94 923.98 903.18 716.88 886.64 931.59 944.72 716.75 863.57 930.90 921.29
Standard Deviation 84.26 121.50 123.36 133.48 93.97 109.64 127.33 130.47 93.00 119.89 127.00 133.77
Intensity Change Log 2 — 0.142570 0.368688 0.335840 — 0.306617 0.377964 0.398155 — 0.226996 0.373337 0.367362

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000088 0.000417 0.000610 0.000881 0.000059 0.000115 0.000267 0.000742 0.000062 0.000187 0.000298 0.000820
Bud Neck 0.001357 0.005687 0.003753 0.021329 0.002361 0.004558 0.014121 0.021309 0.002255 0.004827 0.013181 0.021320
Bud Site 0.000305 0.003076 0.000799 0.006171 0.000776 0.001503 0.001367 0.009279 0.000726 0.001878 0.001316 0.007526
Cell Periphery 0.000103 0.000154 0.000080 0.000171 0.000123 0.000122 0.000092 0.000137 0.000121 0.000129 0.000091 0.000156
Cytoplasm 0.037876 0.032763 0.003671 0.029687 0.020785 0.013318 0.007249 0.024718 0.022593 0.017956 0.006924 0.027520
Cytoplasmic Foci 0.005922 0.003295 0.000966 0.003005 0.000991 0.002167 0.000021 0.001992 0.001513 0.002436 0.000106 0.002563
Eisosomes 0.000023 0.000020 0.000038 0.000023 0.000009 0.000012 0.000016 0.000020 0.000011 0.000014 0.000018 0.000022
Endoplasmic Reticulum 0.002254 0.006986 0.005233 0.005871 0.001684 0.003796 0.003759 0.007162 0.001744 0.004557 0.003893 0.006434
Endosome 0.000290 0.001113 0.000331 0.003909 0.000292 0.001157 0.000149 0.002688 0.000292 0.001146 0.000166 0.003377
Golgi 0.000040 0.000603 0.000068 0.004762 0.000182 0.000632 0.000024 0.000779 0.000167 0.000625 0.000028 0.003025
Lipid Particles 0.000878 0.000885 0.000552 0.000309 0.000366 0.001527 0.000029 0.000262 0.000420 0.001374 0.000076 0.000288
Mitochondria 0.000360 0.003096 0.000702 0.007258 0.001157 0.003971 0.000821 0.004045 0.001073 0.003762 0.000810 0.005857
Mitotic Spindle 0.000014 0.001566 0.000359 0.034426 0.000472 0.005030 0.001519 0.010619 0.000424 0.004203 0.001414 0.024045
None 0.010705 0.007744 0.004854 0.008768 0.005142 0.004387 0.002083 0.005541 0.005731 0.005188 0.002334 0.007361
Nuclear Periphery 0.000458 0.001310 0.000406 0.004032 0.000755 0.001771 0.000684 0.002059 0.000723 0.001661 0.000659 0.003172
Nuclear Periphery Foci 0.000137 0.000467 0.000179 0.001856 0.000097 0.000611 0.000037 0.001151 0.000102 0.000577 0.000049 0.001548
Nucleolus 0.003117 0.004387 0.006492 0.001628 0.002947 0.003288 0.000950 0.001269 0.002965 0.003550 0.001453 0.001471
Nucleus 0.934112* 0.921573* 0.967079* 0.844002* 0.960878* 0.947827* 0.965624* 0.890587* 0.958046* 0.941565* 0.965756* 0.864315*
Peroxisomes 0.000531 0.000784 0.000332 0.000358 0.000216 0.000871 0.000036 0.000542 0.000250 0.000851 0.000063 0.000438
Vacuole 0.001358 0.002985 0.003393 0.016501 0.000569 0.002581 0.001078 0.013012 0.000653 0.002678 0.001288 0.014980
Vacuole Periphery 0.000070 0.001091 0.000102 0.005054 0.000138 0.000756 0.000072 0.002088 0.000131 0.000836 0.000075 0.003761

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.98 -4.69 -7.10 -3.42 -0.18 -3.82 -14.18 -7.05 -6.43 -4.70 -7.85 -13.82 -10.35 -8.35 -6.22
Bud Neck -4.57 -2.69 -13.71 -10.50 -8.03 -3.70 -13.60 -13.90 -11.89 -3.95 -5.09 -13.84 -19.53 -16.15 -5.44
Bud Site -2.89 -2.63 -9.07 -1.73 -6.12 -1.19 -1.25 -5.58 -4.60 -4.91 -2.20 -1.47 -8.60 -5.57 -6.87
Cell Periphery -2.27 0.75 -0.99 1.58 -1.66 0.11 2.19 0.47 0.45 -2.22 -0.57 2.27 0.16 0.96 -2.73
Cytoplasm 0.61 4.85 1.49 1.42 -11.29 3.91 7.59 -1.13 -4.98 -8.47 2.31 8.85 -1.50 -4.41 -12.75
Cytoplasmic Foci 1.37 2.66 2.42 2.22 -1.12 -3.30 5.82 -0.15 2.02 -2.23 -2.36 5.64 0.69 2.94 -4.45
Eisosomes 1.13 -3.48 -0.70 -4.05 3.79 -2.84 -7.04 -13.13 -11.75 -8.69 -3.20 -7.48 -14.09 -13.22 -7.81
Endoplasmic Reticulum -7.01 -5.08 -8.05 0.78 -0.81 -5.43 -13.54 -16.88 -8.04 -10.07 -8.69 -14.27 -22.28 -7.01 -11.41
Endosome -2.66 -1.02 -5.16 -2.12 -4.31 -3.47 1.36 -3.99 -1.25 -4.48 -4.14 1.16 -6.45 -2.41 -7.20
Golgi -1.84 -1.36 -2.96 -1.96 -2.91 -1.27 1.46 -1.15 0.55 -2.39 -1.63 1.43 -2.90 -1.60 -3.35
Lipid Particles -0.08 0.76 2.41 2.29 2.25 -3.55 5.12 0.66 3.74 -8.66 -3.70 5.06 1.59 4.19 -8.67
Mitochondria -2.40 -2.91 -6.74 -1.33 -5.59 -3.77 0.68 -3.95 0.52 -5.17 -4.13 0.47 -6.04 -0.64 -7.69
Mitotic Spindle -2.65 -1.68 -8.68 -7.79 -7.77 -4.32 -2.07 -5.67 -2.65 -4.52 -4.61 -2.26 -10.13 -6.97 -8.63
None 1.05 1.96 1.43 0.64 -1.21 0.88 5.77 0.06 -0.76 -4.70 0.71 5.99 -0.41 -1.09 -6.76
Nuclear Periphery -5.24 -0.88 -8.07 -5.58 -7.68 -4.54 -1.40 -11.07 -3.86 -10.60 -5.39 -1.61 -10.94 -5.77 -10.53
Nuclear Periphery Foci -2.88 -1.00 -3.43 -2.00 -2.55 -2.93 0.90 -2.10 -0.51 -2.19 -3.51 0.43 -4.06 -1.42 -4.13
Nucleolus -1.58 -1.94 2.00 5.26 2.83 -0.62 4.76 1.88 2.58 -2.68 -1.17 3.71 2.65 4.56 -1.78
Nucleus 1.13 -2.35 5.85 6.30 11.69 3.42 -0.58 13.52 10.13 14.21 4.65 -1.47 18.93 13.74 21.09
Peroxisomes -0.54 0.79 1.47 1.49 1.10 -3.24 2.51 -0.51 2.47 -1.85 -3.25 2.69 0.13 3.34 -2.65
Vacuole -2.45 -2.61 -11.40 -10.20 -6.65 -5.37 -4.47 -10.34 -9.13 -9.92 -6.65 -4.94 -16.22 -13.93 -15.17
Vacuole Periphery -2.21 -2.26 -4.38 -1.91 -4.28 -2.74 1.46 -2.68 -0.88 -2.87 -3.43 1.34 -5.01 -2.58 -5.20
Standard name
Human Ortholog
Description Putative checkpoint factor in transcription; binds to U2 snRNA and Prp11p; regulates toggling of the U2 snRNA stem II region between different structures; contains two RNA recognition motifs (RRMs)
Localization
Cell Percentages nucleus (95%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Cus2

Cus2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cus2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available