Standard name
Human Ortholog
Description Cdc42p-activated signal transducing kinase; member of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p; CLA4 has a paralog, SKM1, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.15 0.19 0.16 0.27 0.21 0.12 0.12 0.07 0.06 0.08 0.06 0.09 0.13 0.12 0.08 0.12 0.15 0.14 0.11 0.08 0.07 0.13 0.13 0.1
Bud 0.16 0.19 0.17 0.21 0.17 0.18 0.17 0.16 0.14 0.18 0.15 0.17 0.08 0.07 0.08 0.09 0.08 0.06 0.3 0.31 0.28 0.32 0.29 0.22
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.18 0.2 0.19 0.2 0.1 0.11
Bud Site 0.11 0.1 0.08 0.15 0.05 0.09 0.07 0 0.05 0.06 0 0.06 0.07 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.06 0 0 0 0 0 0 0 0.06 0 0.12 0.21 0.24 0.09 0.16 0.08 0 0 0 0 0 0
Cytoplasm 0.59 0.57 0.6 0.44 0.56 0.57 0.61 0.66 0.67 0.6 0.65 0.63 0.71 0.8 0.83 0.63 0.68 0.7 0.33 0.24 0.31 0.21 0.3 0.42
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.06 0 0 0 0 0.05 0
Endosome 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.08 0.06 0.2 0.28 0.29 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.08 0.09 0.13 0.2 0.18 0.09 0.11 0.11 0.07 0.06 0 0.07 0.05 0 0 0.09 0.08 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.05 0.05 0.05 0.05 0 0 0 0 0 0 0 0 0 0.07 0.09 0.05 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 23 90 57 78 72 42 47 23 23 28 23 41 30 50 33 31 35 28 18 45 26 25 47 44
Bud 25 91 58 62 58 60 64 53 57 62 56 75 17 28 30 23 18 12 54 172 110 63 108 96
Bud Neck 0 5 7 5 6 3 8 8 5 9 9 8 0 0 0 1 10 0 31 111 76 38 38 50
Bud Site 17 49 29 42 18 30 27 7 19 21 16 25 15 6 0 9 7 4 – – – – – –
Cell Periphery 7 11 21 8 12 11 10 8 15 6 24 14 28 86 92 23 36 16 2 4 6 1 7 11
Cytoplasm 93 266 210 126 198 191 237 214 265 211 244 278 160 331 324 160 157 137 58 134 123 41 110 188
Endoplasmic Reticulum 1 7 3 8 3 3 5 0 2 3 2 3 5 3 6 13 17 11 1 5 3 4 18 12
Endosome 8 9 1 5 4 7 5 1 1 1 3 0 11 5 7 8 4 5 0 14 5 2 6 5
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 3 0 0 2 1
Mitochondria 4 20 2 3 6 15 30 20 78 99 107 69 1 1 0 8 3 7 2 10 10 5 5 11
Nucleus 2 7 7 5 9 13 14 4 6 9 4 13 3 0 0 7 3 3 1 0 1 0 1 0
Nuclear Periphery 0 2 1 0 0 0 1 0 0 1 3 0 0 0 2 0 0 0 1 1 2 0 0 1
Nucleolus 1 4 0 0 1 1 0 1 0 0 1 2 0 0 0 2 3 0 0 0 0 0 0 0
Peroxisomes 2 9 1 11 0 6 5 3 2 4 2 0 10 10 1 4 5 1 0 1 0 0 1 1
SpindlePole 13 41 47 58 63 30 43 37 26 23 10 30 11 4 2 23 19 12 1 17 10 2 1 2
Vac/Vac Membrane 1 18 19 15 18 18 7 14 4 9 9 4 1 0 2 18 22 10 1 22 11 9 11 11
Unique Cell Count 158 467 351 289 351 337 387 322 394 354 375 440 226 413 390 252 232 196 179 552 396 198 369 444
Labelled Cell Count 197 629 463 427 468 430 503 393 503 486 513 562 292 524 499 331 339 246 179 552 396 198 369 444


Ambiguous, Bud, Cell Periphery, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.5 6.3 7.1 6.7 6.9 5.6 5.3 5.7 4.7 4.4 4.4 4.9 6.9 7.0 7.2 8.4 8.8 8.4 6.5 6.7 7.0
Std Deviation (1e-4) 1.3 0.9 1.1 1.2 1.7 1.1 0.9 1.3 1.2 0.8 0.9 1.1 1.0 1.2 1.5 1.3 1.4 1.4 1.1 1.6 1.2
Intensity Change (Log2) – – – -0.08 -0.03 -0.34 -0.41 -0.31 -0.58 -0.68 -0.7 -0.54 -0.04 -0.01 0.03 0.25 0.32 0.24 -0.12 -0.09 -0.02

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 3.3 1.5 -1.4 -1.6 -3.6 0 0 0 0 -1.0 -1.6 -3.2 -1.4 -0.4 -0.6
Bud 1.6 0 0.4 0 0 0 0 0 0 -3.1 -4.2 -3.7 -2.6 -3.1 -3.5
Bud Neck -0.2 -0.3 0 0.1 0.4 0 0 0 0 0 0 0 0 1.6 0
Bud Site 2.5 -1.7 0.3 -0.7 -3.5 0 0 0 0 -0.7 -4.5 -5.8 -2.3 -2.6 -2.9
Cell Periphery -1.9 -1.6 -1.7 -2.3 -2.2 0 0 0 0 2.7 5.9 6.7 1.5 3.8 1.0
Cytoplasm -4.1 -0.9 -0.8 0.4 1.8 2.1 -0.1 1.5 1.0 2.7 6.2 7.0 0.9 1.9 2.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.2 4.2 3.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.3 4.8 4.1 0 0 0 0 0 0 0 0 0 0
Nucleus -0.2 0.5 1.5 1.3 -0.8 -0.5 0.5 -1.0 0.9 0 0 0 0.6 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 3.3 0 0 0 0 0 0 0 0 0 2.5 0 0 0 0
SpindlePole 2.3 1.7 -1.9 -0.9 -0.7 0 0 0 0 -3.3 -6.9 -7.0 -1.6 -1.9 -2.6
Vacuole -0.1 -0.2 0 -2.7 -0.6 -3.5 -2.0 -2.1 -3.7 -3.2 -4.8 -4.0 0.9 1.9 -0.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.2196 4.3173 4.4824 4.1026 4.0265 4.8025 3.8284 3.1607 3.438 3.3591 3.1432 3.5376 3.071 2.569 2.8838 2.6911 2.6147 3.0755
Actin 0.0557 0.02 0.0283 0.0388 0.0891 0.0232 0.0598 0.0359 0.0454 0.1314 0.2011 0.0454 0.0657 0.0165 0.0614 0.0566 0.0945 0.0439
Bud 0.0527 0.0746 0.299 0.1051 0.0522 0.0178 0.0521 0.0559 0.3035 0.0965 0.0778 0.0139 0.059 0.0532 0.2666 0.1161 0.0542 0.0101
Bud Neck 0.034 0.0086 0.0028 0.0358 0.0129 0.3249 0.0507 0.0054 0.0036 0.0047 0.0018 0.3977 0.0326 0.006 0.0067 0.0039 0.047 0.466
Bud Periphery 0.1206 0.0802 0.4404 0.4248 0.3303 0.0609 0.1104 0.0624 0.4473 0.5039 0.3318 0.0515 0.094 0.0527 0.4173 0.4361 0.3713 0.0274
Bud Site 0.2228 0.4319 0.0707 0.0191 0.0185 0.0262 0.2171 0.4622 0.0926 0.0499 0.0201 0.0142 0.205 0.3703 0.1042 0.0723 0.0156 0.0195
Cell Periphery 0.029 0.0191 0.0077 0.0211 0.02 0.0087 0.0246 0.0065 0.0059 0.0107 0.0103 0.0107 0.0191 0.0075 0.0069 0.0141 0.015 0.0093
Cytoplasm 0.2968 0.3053 0.0657 0.1322 0.2074 0.4015 0.2552 0.3052 0.0629 0.1439 0.2446 0.3533 0.2829 0.4016 0.0687 0.2827 0.2269 0.3449
Cytoplasmic Foci 0.0185 0.005 0.0087 0.012 0.0314 0.0518 0.0586 0.0137 0.0034 0.002 0.0032 0.028 0.0631 0.0094 0.008 0.0012 0.033 0.0093
Eisosomes 0.0009 0.0003 0.0004 0.003 0.0005 0.0007 0.0006 0.0002 0.0001 0.0007 0.0004 0.0002 0.0008 0.0003 0.0002 0.0002 0.0003 0.0002
Endoplasmic Reticulum 0.0066 0.0019 0.0041 0.0103 0.0057 0.0015 0.0094 0.0015 0.0018 0.0061 0.0056 0.0065 0.0104 0.0026 0.0015 0.0016 0.0238 0.0068
Endosome 0.0178 0.0025 0.0107 0.0312 0.0811 0.0186 0.0384 0.0025 0.0051 0.0089 0.0201 0.0207 0.0212 0.0015 0.0139 0.001 0.0177 0.0112
Golgi 0.0062 0.0025 0.0052 0.018 0.0272 0.0046 0.0145 0.0016 0.0016 0.0056 0.005 0.0123 0.0056 0.0005 0.0079 0.0003 0.0061 0.0121
Lipid Particles 0.0067 0.0003 0.0023 0.0073 0.0174 0.0042 0.0226 0.0007 0.0003 0.0021 0.0021 0.0049 0.0088 0.0003 0.0026 0.0002 0.0189 0.0047
Mitochondria 0.0101 0.0081 0.0062 0.0284 0.0143 0.0023 0.0053 0.0021 0.002 0.0029 0.0081 0.0048 0.0078 0.0003 0.0054 0.0003 0.0135 0.0015
None 0.08 0.0312 0.0328 0.0917 0.0262 0.037 0.0208 0.0305 0.0101 0.0029 0.0521 0.0154 0.0548 0.069 0.0133 0.0022 0.0051 0.0207
Nuclear Periphery 0.0094 0.0004 0.0015 0.0014 0.0051 0.0004 0.0114 0.0004 0.0053 0.0092 0.0065 0.0023 0.0083 0.0004 0.0005 0.0005 0.0211 0.0042
Nucleolus 0.0016 0.0003 0.0011 0.0006 0.0008 0.0004 0.003 0.0008 0.0002 0.0003 0.0002 0.0004 0.0017 0.0003 0.0003 0.0002 0.0017 0.0003
Nucleus 0.0034 0.0017 0.0013 0.0014 0.0017 0.0012 0.0162 0.0025 0.004 0.0016 0.002 0.003 0.0067 0.0015 0.0006 0.0011 0.0041 0.0009
Peroxisomes 0.0078 0.0006 0.0026 0.0055 0.0411 0.0078 0.011 0.0038 0.0014 0.0063 0.0004 0.0051 0.019 0.0003 0.0079 0.0001 0.0065 0.0016
Punctate Nuclear 0.0059 0.0005 0.0021 0.0005 0.003 0.0018 0.007 0.0009 0.0007 0.0005 0.0006 0.001 0.02 0.0007 0.0006 0.0001 0.006 0.0011
Vacuole 0.0122 0.0046 0.0052 0.0104 0.0101 0.0038 0.0095 0.0048 0.0022 0.0069 0.0048 0.0077 0.0121 0.0047 0.004 0.0088 0.0147 0.0037
Vacuole Periphery 0.0015 0.0002 0.001 0.0014 0.0039 0.0005 0.0017 0.0004 0.0005 0.0031 0.0014 0.001 0.0012 0.0002 0.0016 0.0004 0.003 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.1257 12.8709 18.8121 23.9694 12.7279 11.7736 17.9432 21.6923 19.6593 17.6057
Translational Efficiency 0.7314 0.9132 0.8881 0.6846 0.6892 1.0805 0.7809 0.6645 0.9169 0.8185

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1836 1134 1114 1377 1838 1388 1860 2957 3674 2522 2974 4334

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 761.46 909.22 978.08 883.21 801.62 890.37 892.86 954.98 781.55 898.85 924.78 932.18
Standard Deviation 90.36 133.76 136.08 117.56 102.53 117.40 106.72 125.99 98.70 125.37 125.54 127.82
Intensity Change Log 2 — 0.255861 0.361184 0.213988 — 0.151486 0.155515 0.252552 — 0.203277 0.259372 0.233894

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001785 0.003060 0.004499 0.007811 0.002655 0.004238 0.002227 0.004438 0.002221 0.003708 0.003078 0.005509
Bud Neck 0.090589 0.059368 0.029912 0.040575 0.097698 0.072515 0.059328 0.056440 0.094146 0.066603 0.048309 0.051399
Bud Site 0.475605 0.458607 0.207589 0.431086 0.412840 0.468190 0.340760 0.303876 0.444206 0.463881 0.290877 0.344293
Cell Periphery 0.002479 0.001144 0.001043 0.001073 0.001999 0.001670 0.000880 0.000832 0.002238 0.001434 0.000941 0.000908
Cytoplasm 0.329825 0.323736 0.398522 0.355249 0.353938 0.333151 0.353887 0.546131 0.341888 0.328918 0.370606 0.485484
Cytoplasmic Foci 0.041281 0.066298 0.038031 0.015256 0.057797 0.047586 0.023278 0.005846 0.049544 0.056000 0.028804 0.008836
Eisosomes 0.000349 0.000214 0.000188 0.000205 0.000546 0.000222 0.000159 0.000147 0.000448 0.000218 0.000170 0.000166
Endoplasmic Reticulum 0.000900 0.000980 0.004904 0.001445 0.001179 0.000330 0.001064 0.000331 0.001040 0.000622 0.002502 0.000685
Endosome 0.007279 0.012562 0.008662 0.002611 0.008939 0.016045 0.003382 0.001249 0.008109 0.014479 0.005360 0.001682
Golgi 0.003499 0.003466 0.001099 0.002380 0.004040 0.005793 0.000383 0.001021 0.003769 0.004747 0.000651 0.001453
Lipid Particles 0.000992 0.001420 0.003694 0.000729 0.001753 0.001414 0.001276 0.000229 0.001372 0.001417 0.002182 0.000388
Mitochondria 0.002238 0.001713 0.000413 0.002001 0.001277 0.003775 0.000432 0.001961 0.001758 0.002848 0.000425 0.001974
Mitotic Spindle 0.001239 0.001657 0.004035 0.007170 0.001243 0.002384 0.004171 0.008412 0.001241 0.002057 0.004120 0.008018
None 0.001184 0.000449 0.001792 0.000403 0.001041 0.000106 0.002662 0.000720 0.001113 0.000260 0.002336 0.000620
Nuclear Periphery 0.000022 0.000028 0.000811 0.000083 0.000029 0.000016 0.000207 0.000050 0.000025 0.000022 0.000433 0.000061
Nuclear Periphery Foci 0.000181 0.000294 0.005426 0.000577 0.000270 0.000116 0.000568 0.000061 0.000225 0.000196 0.002388 0.000225
Nucleolus 0.000160 0.000111 0.000612 0.000168 0.000210 0.000053 0.000257 0.000103 0.000185 0.000079 0.000390 0.000124
Nucleus 0.005047 0.003774 0.082494 0.026802 0.007823 0.001858 0.052921 0.020267 0.006436 0.002720 0.063999 0.022344
Peroxisomes 0.002336 0.003645 0.002665 0.002856 0.002814 0.005238 0.003024 0.002449 0.002575 0.004522 0.002889 0.002578
Vacuole 0.032021 0.055767 0.203255 0.100831 0.040990 0.033705 0.148750 0.045077 0.036508 0.043625 0.169167 0.062791
Vacuole Periphery 0.000989 0.001709 0.000356 0.000688 0.000920 0.001593 0.000382 0.000359 0.000954 0.001645 0.000372 0.000463

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.90 -3.24 -5.41 -3.19 -2.90 -2.30 1.86 -1.93 0.67 -3.02 -3.07 -1.22 -5.07 -1.28 -3.94
Bud Neck 5.89 15.61 14.14 6.90 -2.26 4.61 9.73 11.90 6.41 1.59 7.22 16.05 17.26 8.54 0.05
Bud Site 1.38 28.43 13.27 10.22 -13.21 -4.42 10.91 18.10 21.53 5.22 -1.98 24.89 24.45 23.74 -3.14
Cell Periphery 4.31 5.36 5.36 2.12 -0.23 0.58 6.76 6.44 1.82 1.00 2.33 8.16 8.04 2.27 0.79
Cytoplasm 0.43 -3.25 -4.02 -4.04 -0.57 1.88 -0.56 -22.41 -22.50 -21.86 1.54 -2.72 -22.39 -21.90 -18.74
Cytoplasmic Foci -6.14 1.73 10.41 13.06 8.56 2.61 11.89 19.61 13.93 13.32 -2.38 10.69 23.11 19.75 16.02
Eisosomes 3.67 5.49 4.46 1.02 -0.46 6.36 8.68 8.45 2.76 1.07 7.24 10.32 9.57 3.05 0.83
Endoplasmic Reticulum -0.21 -9.79 -2.37 -2.12 8.83 3.21 0.00 2.84 -0.54 4.95 1.94 -7.27 1.24 -1.14 10.33
Endosome -4.44 -0.68 7.17 9.65 7.61 -4.22 7.21 10.67 9.74 7.49 -5.96 5.38 13.37 13.43 10.77
Golgi 0.08 4.45 2.59 2.54 -1.04 -1.95 9.00 7.27 6.23 -2.22 -1.78 9.84 7.50 7.12 -1.76
Lipid Particles -1.43 -10.34 -0.31 1.23 9.90 0.66 1.21 6.37 2.30 5.78 -0.15 -4.96 6.11 2.95 11.21
Mitochondria 0.65 3.04 0.96 0.35 -1.58 -2.79 4.29 -1.11 2.39 -4.98 -1.73 4.25 0.52 2.16 -4.41
Mitotic Spindle -0.88 -3.91 -4.18 -3.74 -1.06 -1.48 -3.32 -5.39 -4.35 -2.58 -1.76 -5.07 -6.94 -5.83 -2.73
None 3.12 -1.59 2.64 -1.04 4.37 3.46 -1.88 0.54 -11.85 2.25 4.81 -2.21 1.77 -7.01 3.09
Nuclear Periphery -0.95 -12.34 -11.47 -11.07 10.08 1.41 -8.87 -9.24 -12.76 4.64 0.59 -14.77 -14.81 -16.53 10.99
Nuclear Periphery Foci -2.32 -11.75 -3.15 -2.71 8.79 3.06 -5.48 3.52 0.68 9.55 0.87 -12.51 -1.88 -2.27 10.75
Nucleolus 1.30 -2.51 -0.77 -1.04 1.14 3.67 -0.66 2.00 -2.54 2.52 3.79 -2.57 0.20 -1.59 2.24
Nucleus 2.79 -23.93 -17.12 -17.66 11.66 10.37 -20.27 -20.32 -25.43 7.00 10.26 -30.97 -27.21 -30.65 13.28
Peroxisomes -2.48 0.48 0.23 2.51 -0.24 -3.28 0.20 2.10 4.26 1.22 -4.14 0.22 1.75 4.93 1.05
Vacuole -6.26 -30.42 -22.78 -17.78 8.15 2.59 -25.53 -14.60 -16.27 15.90 -3.27 -38.86 -26.45 -22.18 18.15
Vacuole Periphery -1.76 6.52 2.55 2.83 -1.21 -2.10 3.09 5.07 4.14 0.24 -2.71 5.09 5.84 5.05 -0.28
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Cdc42p-activated signal transducing kinase; member of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p; CLA4 has a paralog, SKM1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (51%), bud neck (21%), mixed (24%)
Cell Cycle Regulation Yes
cytoplasm - T/G1 (7.5e-05)
bud neck - S/G2 (1.2e-06)
Subcompartmental Group N/A

Cla4

Cla4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cla4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available