Standard name
Human Ortholog
Description Non-canonical poly(A) polymerase; involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0.07 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0.06 0.25 0.51 0.49 0.54 0.32 0.36 0.24 0.33 – 0.22 0.3 0.15 0.05 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.05 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.16 0 0 0 0 0 0 0 – 0 0.09 0.05 0.05 0.05 0.07 0.05 0.05 0 0.08 0.11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.4 0 0.08 0.1 0 0.17 0.3 0.26 0.59 0.46 0.74 0.54 – 0 0 0.2 0 0.08 0 0 0 0.06 0 0
Nucleus 0.63 0.73 0.96 0.63 0.27 0.14 0.12 0.13 0.17 0.12 0.1 0.12 – 0.38 0.35 0.42 0.53 0.44 0.31 0.44 0.43 0.49 0.31 0.19
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0
Nucleolus 0.27 0.14 0 0.27 0 0 0 0 0 0 0 0 – 0.28 0.14 0.31 0.32 0.22 0.41 0.3 0.22 0.31 0.26 0.24
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.26 0.22 0.08 0.09 0.13 0 0.08 0.07 0.14 – 0.25 0.25 0 0 0 0.1 0.07 0.08 0 0.07 0.13
Vac/Vac Membrane 0 0.08 0 0 0.17 0.17 0.05 0 0.05 0.06 0 0.06 – 0 0.05 0.09 0.32 0.23 0 0.09 0.13 0 0.17 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0
Bud 2 0 0 1 0 1 6 10 3 11 12 13 – 0 0 0 0 0 0 3 1 0 1 1
Bud Neck 0 0 0 0 2 3 5 18 5 10 4 5 – 1 0 0 0 0 1 1 0 0 1 2
Bud Site 0 0 0 0 0 0 2 3 3 5 5 6 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 12 0 6 44 44 95 140 51 83 59 94 – 15 31 8 1 10 0 3 1 0 2 2
Endoplasmic Reticulum 2 0 0 0 0 0 1 0 0 1 1 1 – 1 0 3 1 4 0 0 0 0 0 0
Endosome 1 11 0 4 28 2 3 5 1 7 0 5 – 2 9 3 1 6 14 14 3 0 7 15
Golgi 0 1 0 1 1 1 0 0 0 0 0 0 – 0 0 1 0 0 1 1 0 0 0 0
Mitochondria 74 10 2 10 4 15 58 67 93 105 179 153 – 2 1 11 0 9 4 4 2 1 2 5
Nucleus 116 194 23 61 49 12 23 33 26 28 23 33 – 26 36 23 10 49 64 139 30 14 27 25
Nuclear Periphery 2 0 0 0 0 0 0 0 0 0 1 1 – 0 0 0 1 1 2 0 0 1 0 1
Nucleolus 50 36 0 26 2 0 0 1 0 0 1 3 – 19 14 17 6 24 85 96 15 8 22 32
Peroxisomes 0 0 0 0 0 0 2 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 3 11 0 25 39 7 18 33 5 19 18 39 – 17 26 2 0 4 20 21 5 0 5 16
Vac/Vac Membrane 2 20 1 0 31 15 9 10 8 14 6 16 – 1 5 5 6 25 8 27 9 0 15 26
Unique Cell Count 185 264 24 97 179 87 192 261 157 229 242 285 69 102 55 19 111 208 317 71 29 89 135
Labelled Cell Count 256 295 26 134 200 100 222 322 195 284 309 369 84 122 73 26 132 208 317 71 29 89 135


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 5.7 5.2 6.1 5.7 4.6 4.4 4.5 3.6 3.9 3.4 3.7 – 5.9 5.4 8.0 9.2 8.3 – 6.4 6.3
Std Deviation (1e-4) 0.7 1.0 0.5 1.3 1.3 1.2 1.0 1.1 0.7 1.4 1.2 0.7 – 1.3 1.0 2.1 3.0 2.1 – 1.2 1.5
Intensity Change (Log2) – – – 0.24 0.15 -0.17 -0.25 -0.2 -0.51 -0.4 -0.59 -0.49 – 0.18 0.05 0.62 0.82 0.67 – 0.3 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cytoplasm 0 2.7 4.5 4.6 5.0 3.3 3.6 2.7 3.4 – 0 3.1 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleus -3.1 -6.6 -7.7 -9.5 0 -8.1 0 0 0 – -4.9 -5.3 -4.5 -3.3 -4.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleolus 2.9 0 0 0 0 0 0 0 0 – 0 0 3.1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.173 0.3626 0.3053 -0.3344 0.0552 0.2593 0.5787 1.1717 0.8377 0.8912 1.0225 1.0669 -0.5831 -0.2065 -0.5192 -0.3872 0.8711 -0.4243
Actin 0.0335 0 0.0085 0.0001 0.0021 0.0007 0.0116 0 0.0067 0.0111 0.0168 0.0011 0.0118 0 0.0186 0.0135 0.0278 0.0033
Bud 0.0006 0.0001 0.0004 0.0002 0.0007 0.0003 0.0004 0.0001 0.0003 0.0002 0.0011 0.0002 0.0005 0.0002 0.0008 0.0007 0.0016 0.0003
Bud Neck 0.0051 0.002 0.0028 0.0036 0.0055 0.0071 0.0047 0.0046 0.0024 0.0028 0.0062 0.0052 0.0045 0.0021 0.0022 0.0031 0.0025 0.0059
Bud Periphery 0.0015 0.0002 0.001 0.0005 0.0012 0.001 0.0012 0.0002 0.0008 0.0003 0.0048 0.0007 0.0013 0.0005 0.0011 0.0025 0.0017 0.0007
Bud Site 0.0042 0.0025 0.0031 0.0021 0.0035 0.0013 0.0015 0.002 0.0057 0.0015 0.0026 0.002 0.0067 0.006 0.0094 0.0024 0.0036 0.0014
Cell Periphery 0.0008 0.0003 0.0007 0.0005 0.0005 0.0003 0.0004 0.0006 0.0005 0.0003 0.0007 0.0004 0.0009 0.0006 0.0006 0.0008 0.0004 0.0007
Cytoplasm 0.0068 0.0013 0.0012 0.0052 0.0039 0.0024 0.0054 0.0031 0.0005 0.0006 0.0025 0.0005 0.0209 0.0011 0.0016 0.0004 0.0149 0.001
Cytoplasmic Foci 0.0202 0.0012 0.0058 0.0039 0.013 0.0053 0.0112 0.0074 0.0067 0.026 0.0057 0.0066 0.0227 0.0061 0.0072 0.002 0.0477 0.013
Eisosomes 0.0002 0 0.0001 0.0001 0.0001 0.0001 0.0002 0 0.0001 0.0001 0.0016 0 0.0004 0 0.0004 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0023 0.0004 0.0008 0.0011 0.0013 0.0015 0.0048 0.0001 0.0002 0.0003 0.0026 0.0002 0.0037 0.0004 0.001 0.0011 0.0033 0.0004
Endosome 0.0217 0.0025 0.0084 0.0142 0.037 0.0119 0.0263 0.0031 0.0108 0.0106 0.04 0.0107 0.0194 0.0059 0.0139 0.0083 0.0856 0.0061
Golgi 0.0092 0.0003 0.0019 0.0008 0.0115 0.0015 0.0041 0.0005 0.0057 0.0139 0.0265 0.0099 0.0064 0.0006 0.009 0.0057 0.0229 0.0036
Lipid Particles 0.0431 0.0043 0.0128 0.0179 0.0449 0.0184 0.0377 0.0047 0.0124 0.0402 0.0165 0.0116 0.0338 0.0147 0.0226 0.0127 0.0602 0.0297
Mitochondria 0.007 0.0002 0.0152 0.0007 0.0252 0.0181 0.0041 0.0002 0.0086 0.0043 0.0597 0.0042 0.0067 0.0006 0.0203 0.0027 0.0088 0.0123
None 0.0106 0.0129 0.0041 0.0042 0.0022 0.0192 0.0184 0.0007 0.0006 0.0007 0.0259 0.0006 0.0028 0.0009 0.0132 0.0004 0.0024 0.0097
Nuclear Periphery 0.0223 0.0081 0.0112 0.0073 0.0799 0.015 0.0283 0.0014 0.0012 0.0018 0.0031 0.0041 0.0105 0.0172 0.0067 0.0091 0.044 0.0037
Nucleolus 0.4285 0.5728 0.5911 0.668 0.3771 0.5129 0.5035 0.6708 0.6581 0.63 0.4757 0.568 0.5054 0.5865 0.6293 0.5359 0.3368 0.5553
Nucleus 0.2613 0.3296 0.2698 0.1941 0.2959 0.32 0.2614 0.2667 0.2525 0.204 0.2628 0.2788 0.3013 0.3012 0.2021 0.371 0.2302 0.2737
Peroxisomes 0.0096 0.0002 0.0018 0.001 0.0042 0.0027 0.0085 0.0005 0.0072 0.019 0.0029 0.0101 0.0065 0.0015 0.0115 0.0013 0.0185 0.0162
Punctate Nuclear 0.099 0.0573 0.0483 0.0473 0.0736 0.0512 0.0539 0.0264 0.0112 0.0296 0.0376 0.0781 0.0209 0.0485 0.0227 0.0078 0.0818 0.058
Vacuole 0.0095 0.0032 0.009 0.0256 0.0118 0.007 0.0088 0.0064 0.0064 0.0022 0.0032 0.0061 0.0097 0.0044 0.0043 0.0156 0.0031 0.0043
Vacuole Periphery 0.0029 0.0005 0.0022 0.0018 0.0047 0.0022 0.0037 0.0003 0.0013 0.0005 0.0015 0.0009 0.0034 0.0008 0.0014 0.0031 0.0021 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.7343 14.8289 12.142 11.6398 15.2035 13.1489 13.3132 8.7238 10.8379 13.2591
Translational Efficiency 0.4167 0.9758 0.99 0.7883 0.5472 0.403 0.6836 0.7943 0.6275 0.626

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✔
Standard name
Human Ortholog
Description Non-canonical poly(A) polymerase; involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p
Localization
Cell Percentages nucleus (41%), nucleolus (30%), mixed (25%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Trf5

Trf5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Trf5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available