Standard name
Human Ortholog
Description Protein with role in regulation of MBF-specific transcription at Start; phosphorylated by Cln-Cdc28p kinases in vitro; unphosphorylated form binds Swi6p, which is required for Stb1p function; expression is cell-cycle regulated; STB1 has a paralog, YOL131W, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.09 0.05 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.42 0.47 0.51 0.5 0.66 0.33 0.28 0.27 0.19 0.18 0.13 0.15 0.57 0.41 0.51 0.49 0.53 0.52 0.31 0.3 0.36 0.05 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0.05 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.23 0.15 0.14 0.19 0.13 0.52 0.49 0.47 0.54 0.61 0.59 0.68 0 0 0 0.06 0 0 0 0 0 0 0 0
Nucleus 0.49 0.41 0.39 0.44 0.21 0.39 0.39 0.4 0.48 0.42 0.49 0.45 0.36 0.35 0.35 0.45 0.4 0.39 0.39 0.38 0.25 0.7 0.63 0.58
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.11 0 0 0 0 0.07 0.11 0.08 0.13 0.14 0.15 0.16 0 0 0 0 0 0.06 0.1 0.07 0.07 0.1 0.17 0.13
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.07 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0.21 0.14 0 0.05 0 0 0.06 0.06 0 0.08 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 2
Bud 3 0 2 1 3 2 5 4 8 4 5 7 2 2 2 0 0 0 18 14 7 15 4 12
Bud Neck 0 0 0 0 0 0 2 0 0 1 1 1 0 0 0 0 0 0 0 1 2 2 0 5
Bud Site 0 0 0 0 1 1 3 3 4 8 7 6 0 0 0 1 0 0
Cell Periphery 0 0 0 1 2 0 4 1 5 1 4 3 0 0 2 3 7 1 0 0 0 0 0 0
Cytoplasm 113 74 70 86 193 89 100 125 64 80 47 71 266 281 344 212 137 72 82 50 54 21 6 4
Endoplasmic Reticulum 0 2 0 0 0 0 0 1 3 0 1 1 1 2 2 20 10 6 0 1 2 1 2 2
Endosome 1 0 1 0 2 0 9 7 0 0 0 0 4 48 21 9 4 2 3 1 7 3 12 17
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 2 1 1 0 0 8 3
Mitochondria 61 23 19 32 39 138 176 217 182 272 209 325 12 17 11 25 10 5 3 0 4 2 6 8
Nucleus 130 65 53 76 62 103 139 183 164 188 174 215 169 240 234 191 104 54 104 64 38 284 345 365
Nuclear Periphery 3 0 0 1 2 1 10 6 4 9 5 10 1 0 0 1 1 0 0 0 0 0 2 4
Nucleolus 29 2 5 2 9 19 38 36 44 64 54 74 11 16 6 14 4 8 27 11 10 41 91 84
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 0 0 0 0 2 1 5 4 2 0 1 2 3 4 2 3 6 1 13 12 10 16 17 27
Vac/Vac Membrane 5 8 2 3 10 9 13 18 7 17 14 14 22 143 95 8 12 6 8 9 9 12 43 81
Unique Cell Count 266 157 136 171 292 266 359 458 339 443 356 477 468 687 672 429 260 138 270 170 152 408 549 634
Labelled Cell Count 345 174 152 202 325 363 505 605 487 644 522 730 493 753 719 487 295 157 270 170 152 408 549 634


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 5.2 5.0 4.8 5.0 4.5 4.2 4.5 4.5 4.3 4.4 4.2 5.6 5.6 5.5 6.6 7.5 8.7 5.3 5.4 5.8
Std Deviation (1e-4) 0.6 1.0 1.1 1.2 1.3 1.1 1.1 1.3 1.1 1.3 1.0 1.1 0.9 1.2 1.1 1.3 1.2 1.6 1.1 1.6 1.6
Intensity Change (Log2) -0.07 0.0 -0.17 -0.27 -0.15 -0.15 -0.23 -0.2 -0.25 0.15 0.16 0.12 0.39 0.57 0.79 0.07 0.11 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.2 2.9 -3.5 -4.9 -5.3 -7.1 -7.8 -8.9 -8.9 1.1 -2.3 -0.1 -0.4 0.2 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 2.8 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -0.2 0 0 0 0 0 0 0 -5.3 -6.0 -6.9 -3.1 -3.7 -3.0
Nucleus 1.0 -3.9 0 -0.1 0.2 1.9 0.7 2.0 1.3 -0.6 -0.9 -0.9 1.1 0.2 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 1.7 5.4 4.1 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6639 1.7993 1.7942 1.6903 1.2551 1.7328 1.2113 1.4254 1.4883 1.3646 1.5575 1.2533 3.261 3.1011 3.192 3.1456 3.0615 3.0736
Actin 0.0085 0.0022 0.0052 0.0006 0.0019 0.0035 0.0417 0.0028 0.0063 0.0044 0.0181 0.0074 0.0342 0.0017 0.0161 0.0073 0.0208 0.0114
Bud 0.0013 0.0273 0.0354 0.0107 0.0169 0.0088 0.0005 0.0331 0.0311 0.0194 0.0384 0.0095 0.0011 0.043 0.0361 0.0525 0.0074 0.0117
Bud Neck 0.0025 0.0054 0.0094 0.0055 0.01 0.017 0.0013 0.0029 0.0172 0.0054 0.0055 0.0144 0.0103 0.004 0.0046 0.0083 0.0067 0.019
Bud Periphery 0.0013 0.0169 0.0274 0.0098 0.0292 0.0128 0.001 0.0242 0.0309 0.0179 0.0309 0.0123 0.0026 0.0284 0.0341 0.0654 0.0105 0.0136
Bud Site 0.0054 0.0584 0.0524 0.009 0.0091 0.0045 0.0021 0.0605 0.0373 0.0078 0.0072 0.0041 0.003 0.0998 0.0495 0.0231 0.004 0.0041
Cell Periphery 0.0002 0.0016 0.002 0.0009 0.0006 0.0004 0.0002 0.002 0.0022 0.0012 0.001 0.0004 0.0002 0.0026 0.0017 0.0021 0.0008 0.0005
Cytoplasm 0.0381 0.3042 0.4385 0.4725 0.3809 0.0623 0.0466 0.3293 0.4501 0.4712 0.3959 0.0938 0.0224 0.3945 0.5471 0.5658 0.4865 0.0842
Cytoplasmic Foci 0.0058 0.0052 0.0106 0.019 0.0109 0.0022 0.0234 0.0088 0.014 0.0151 0.0191 0.0056 0.0107 0.0111 0.0113 0.0144 0.0111 0.0038
Eisosomes 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0005 0.0001 0.0006 0.0001 0.0004 0.0001 0.0002 0.0001 0.0003 0.0001 0.0017 0.0001
Endoplasmic Reticulum 0.0014 0.0046 0.007 0.0062 0.0077 0.0009 0.0034 0.0047 0.0061 0.0069 0.0083 0.002 0.0016 0.0075 0.0065 0.0066 0.0062 0.0011
Endosome 0.0039 0.0044 0.0151 0.0266 0.0205 0.0011 0.024 0.0085 0.0085 0.0443 0.0319 0.0094 0.0124 0.0075 0.0088 0.0146 0.0091 0.0034
Golgi 0.0007 0.0004 0.0014 0.0008 0.0004 0.0003 0.0079 0.0006 0.0014 0.005 0.03 0.0057 0.0057 0.0008 0.0029 0.0009 0.0022 0.0013
Lipid Particles 0.001 0.0004 0.0048 0.0009 0.0007 0.0001 0.0143 0.0006 0.0026 0.0078 0.0134 0.0078 0.0136 0.0008 0.0058 0.0008 0.0025 0.0016
Mitochondria 0.001 0.0017 0.0024 0.0016 0.0028 0.0011 0.0064 0.0017 0.0128 0.0095 0.1004 0.0059 0.008 0.0017 0.0158 0.0025 0.0027 0.0018
None 0.0195 0.086 0.0828 0.1742 0.1739 0.0241 0.0373 0.1567 0.1782 0.1956 0.0943 0.043 0.0248 0.0663 0.0934 0.0968 0.244 0.013
Nuclear Periphery 0.0051 0.0167 0.0089 0.0048 0.0105 0.0027 0.019 0.0048 0.0062 0.0107 0.0176 0.0044 0.0054 0.0076 0.007 0.0066 0.0089 0.0062
Nucleolus 0.0216 0.0173 0.014 0.01 0.0343 0.0394 0.0135 0.009 0.0105 0.0042 0.005 0.0301 0.0129 0.0198 0.0059 0.0051 0.0095 0.0345
Nucleus 0.8306 0.3992 0.246 0.1909 0.2275 0.7876 0.715 0.3306 0.1411 0.0947 0.1458 0.7253 0.8059 0.267 0.1148 0.0933 0.125 0.7607
Peroxisomes 0.0013 0.0005 0.0009 0.0004 0.0005 0.0002 0.0244 0.0004 0.0136 0.0029 0.0128 0.0081 0.0104 0.0007 0.0068 0.0011 0.0017 0.0023
Punctate Nuclear 0.0486 0.0304 0.0077 0.0032 0.0274 0.0279 0.0108 0.0049 0.0077 0.0033 0.0025 0.0072 0.0125 0.0126 0.0129 0.004 0.0234 0.0212
Vacuole 0.0018 0.0156 0.0258 0.0486 0.0307 0.0027 0.0041 0.0123 0.019 0.0649 0.0167 0.003 0.0017 0.0208 0.0161 0.0261 0.0139 0.0041
Vacuole Periphery 0.0003 0.0014 0.0026 0.0041 0.0034 0.0003 0.0025 0.0013 0.0025 0.0077 0.0047 0.0007 0.0006 0.0018 0.0024 0.0028 0.0016 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.2887 5.4669 6.9542 16.5611 17.557 22.702 6.3172 9.3268 7.7896 17.6034
Translational Efficiency 2.9619 2.2619 2.1482 1.4755 1.8131 2.3722 2.0837 1.2829 2.7082 1.9666

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein with role in regulation of MBF-specific transcription at Start; phosphorylated by Cln-Cdc28p kinases in vitro; unphosphorylated form binds Swi6p, which is required for Stb1p function; expression is cell-cycle regulated; STB1 has a paralog, YOL131W, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (61%), cytoplasm (15%), mixed (20%)
Cell Cycle Regulation Yes
nucleus - T/G1 (8.4e-05)
Subcompartmental Group N/A

Stb1

Stb1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Stb1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available