Standard name
Human Ortholog
Description F-box protein of unknown function; predicted to be part of an SCF ubiquitin protease complex; involved in regulating protein levels of sulfur metabolism enzymes; may interact with ribosomes, based on co-purification experiments
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 – 0 0.05 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.99 0.97 0.97 0.99 0.98 0.91 0.94 0.93 0.87 – 0.82 0.82 0.99 0.98 0.99 – – – 0.96 0.9 0.85 0.93 0.93 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.14 0.07 0.26 – 0.26 0.23 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0.1 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 – 0 1 0 0 0 – – – 0 1 0 0 0 2
Bud 1 2 1 0 1 2 2 3 8 – 6 6 0 2 0 – – – 0 2 0 0 1 4
Bud Neck 0 0 0 0 0 0 0 3 0 – 1 1 0 0 0 – – – 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – – – – –
Cell Periphery 6 8 0 6 9 4 2 5 1 – 1 1 3 9 4 – – – 0 0 0 0 0 0
Cytoplasm 389 194 188 167 232 138 259 237 136 – 133 109 349 380 433 – – – 380 196 182 87 288 374
Endoplasmic Reticulum 3 2 3 0 1 0 0 0 0 – 1 2 0 0 3 – – – 0 0 1 1 6 6
Endosome 1 0 0 0 0 0 1 0 0 – 0 1 0 0 0 – – – 1 2 1 0 0 1
Golgi 0 0 0 0 0 0 0 0 0 – 1 0 0 0 0 – – – 2 5 0 0 0 0
Mitochondria 1 1 0 4 2 14 38 18 41 – 43 30 2 3 1 – – – 2 1 2 1 2 2
Nucleus 2 0 7 0 3 1 2 4 0 – 3 2 0 1 1 – – – 0 0 1 0 0 1
Nuclear Periphery 0 1 0 0 0 0 0 0 0 – 1 1 0 0 1 – – – 2 0 0 0 1 0
Nucleolus 0 1 0 0 0 0 0 0 1 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 2 0 – 1 0 0 0 0 – – – 1 0 0 0 0 2
Vac/Vac Membrane 0 0 0 2 1 2 2 1 1 – 0 0 1 5 1 – – – 2 1 22 0 5 10
Unique Cell Count 394 200 193 169 236 151 276 254 157 163 133 351 387 439 395 217 215 94 311 413
Labelled Cell Count 403 209 199 179 249 161 306 274 188 191 154 355 400 444 395 217 215 94 311 413


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.6 8.7 6.9 6.7 6.7 5.4 4.8 5.0 4.1 – 3.8 4.0 7.3 7.3 6.8 – – – 6.9 7.0 7.1
Std Deviation (1e-4) 1.2 1.4 0.9 1.1 1.2 1.8 1.0 1.3 0.8 – 0.8 0.8 1.1 1.3 1.2 – – – 0.9 1.1 1.2
Intensity Change (Log2) – – – -0.05 -0.05 -0.36 -0.54 -0.46 -0.77 – -0.88 -0.78 0.09 0.08 -0.03 – – – 0.0 0.02 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Cytoplasm 1.0 0.6 -2.5 -1.8 -2.0 -3.8 – -5.0 -4.8 2.0 0.6 1.1 – – –
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Mitochondria 0 0 4.3 0 3.8 0 – 0 0 0 0 0 – – –
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 – – –
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.1531 1.4339 0.2411 -0.6089 0.0701 0.5731 3.0089 4.0856 3.3325 3.0136 2.7837 3.46 2.3429 3.1704 2.5869 2.819 2.1122 2.8993
Actin 0.0256 0.0004 0.0099 0.0008 0.0251 0.0018 0.0363 0.0044 0.0035 0.0094 0.0346 0.0067 0.0125 0.0005 0.0333 0.0003 0.015 0.002
Bud 0.0014 0.0001 0.001 0.0002 0.0014 0.0021 0.0009 0.0004 0.0004 0.001 0.0006 0.0004 0.0012 0.0016 0.0008 0.0002 0.0015 0.0001
Bud Neck 0.0018 0.0001 0.002 0.0002 0.0007 0.0017 0.0093 0.0002 0.0043 0.0016 0.001 0.0011 0.0006 0.0001 0.0014 0.001 0.0023 0.0004
Bud Periphery 0.003 0.0001 0.0023 0.0001 0.0036 0.0097 0.001 0.0003 0.0003 0.001 0.0007 0.0004 0.0011 0.0008 0.0011 0.0001 0.0033 0.0002
Bud Site 0.0045 0.0007 0.0051 0.0002 0.0007 0.0015 0.0103 0.0022 0.0022 0.0028 0.0021 0.0007 0.002 0.0018 0.0034 0.0034 0.001 0.0001
Cell Periphery 0.001 0.0003 0.0003 0.0001 0.0002 0.0005 0.0007 0.0003 0.0001 0.0003 0.0001 0.0001 0.0002 0.0002 0.0003 0.0004 0.0002 0.0001
Cytoplasm 0.5384 0.8066 0.6541 0.4437 0.4786 0.7851 0.7245 0.9597 0.9255 0.8883 0.7544 0.944 0.7253 0.8308 0.7474 0.8882 0.7234 0.8633
Cytoplasmic Foci 0.0246 0.0031 0.0223 0.0153 0.0687 0.0096 0.0207 0.0036 0.016 0.022 0.0183 0.0104 0.0241 0.0118 0.0154 0.0176 0.0178 0.0052
Eisosomes 0.0006 0 0.0004 0 0.0003 0 0.001 0.0002 0 0 0.0002 0 0.0002 0 0.0003 0 0.0002 0
Endoplasmic Reticulum 0.0135 0.0039 0.0083 0.0028 0.0092 0.0061 0.01 0.0017 0.0027 0.0128 0.0023 0.0025 0.0075 0.0024 0.0063 0.0036 0.0031 0.002
Endosome 0.0394 0.0012 0.0365 0.0302 0.1389 0.0159 0.0436 0.0024 0.0102 0.0093 0.0167 0.01 0.0289 0.0057 0.0136 0.0191 0.0546 0.0134
Golgi 0.006 0.0001 0.0071 0.0003 0.0179 0.0031 0.0077 0.0012 0.0065 0.0093 0.0355 0.0027 0.0063 0.0011 0.0057 0.0033 0.0203 0.0037
Lipid Particles 0.0053 0.0002 0.0185 0.0003 0.0446 0.0051 0.0065 0.0026 0.0039 0.0051 0.0591 0.0004 0.0035 0.0005 0.0031 0.0237 0.0177 0.0008
Mitochondria 0.0044 0.0001 0.005 0.0002 0.0296 0.0027 0.0063 0.0032 0.0036 0.0012 0.037 0.0004 0.0044 0.0002 0.0027 0.0009 0.0302 0.0046
None 0.2873 0.1803 0.1948 0.4684 0.089 0.1461 0.0645 0.014 0.011 0.0271 0.0048 0.0107 0.1694 0.1399 0.1446 0.024 0.0877 0.1007
Nuclear Periphery 0.0102 0.0006 0.0076 0.0011 0.0232 0.0016 0.0155 0.0005 0.0004 0.0006 0.0004 0.0022 0.002 0.0004 0.0043 0.0008 0.0016 0.0007
Nucleolus 0.0075 0 0.0008 0.0002 0.0007 0.0002 0.0105 0.0001 0.0001 0.0001 0.0002 0.0002 0.0003 0.0001 0.0005 0.0009 0.0003 0
Nucleus 0.0071 0.0008 0.0116 0.0021 0.0023 0.0012 0.0139 0.0007 0.0006 0.0009 0.0005 0.0044 0.0013 0.0006 0.0024 0.0013 0.001 0.0005
Peroxisomes 0.0062 0 0.0019 0.0001 0.0411 0.0007 0.0036 0.0009 0.0069 0.0039 0.0291 0.0002 0.0035 0.0003 0.009 0.0014 0.0083 0.0002
Punctate Nuclear 0.0029 0.0002 0.0039 0.0008 0.0097 0.0011 0.0066 0.0002 0.0008 0.0005 0.0006 0.0018 0.0027 0.0003 0.0022 0.0015 0.0018 0.0002
Vacuole 0.0071 0.001 0.005 0.0311 0.0098 0.0038 0.0048 0.0012 0.0009 0.0026 0.0011 0.0006 0.0024 0.0006 0.0019 0.0076 0.0052 0.0012
Vacuole Periphery 0.0021 0.0001 0.0016 0.002 0.0047 0.0007 0.0019 0.0002 0.0002 0.0003 0.0006 0.0002 0.0006 0.0001 0.0006 0.0006 0.0035 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.554 8.6071 8.6223 14.0133 8.1143 8.6607 12.7276 13.3627 12.954 14.9216
Translational Efficiency 0.4384 0.5486 0.5815 0.48 0.5972 0.5809 0.4333 0.343 0.4115 0.4636

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2180 1282 676 1454 1282 1482 887 1291 3462 2764 1563 2745

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 781.58 999.82 1006.10 924.20 779.03 993.47 952.14 894.34 780.64 996.42 975.48 910.16
Standard Deviation 90.32 148.79 172.01 122.76 136.27 134.14 123.74 117.80 109.61 141.16 149.00 121.37
Intensity Change Log 2 — 0.355275 0.364308 0.241812 — 0.350798 0.289495 0.199145 — 0.353042 0.327448 0.220671

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000133 0.001090 0.000689 0.002415 0.000181 0.000748 0.000151 0.000636 0.000151 0.000907 0.000384 0.001578
Bud Neck 0.005534 0.004063 0.003668 0.004648 0.002688 0.005755 0.001920 0.005943 0.004480 0.004970 0.002676 0.005257
Bud Site 0.008732 0.017614 0.009556 0.067581 0.010034 0.020085 0.006947 0.030690 0.009214 0.018939 0.008075 0.050231
Cell Periphery 0.000149 0.000089 0.000181 0.000135 0.000099 0.000097 0.000150 0.000080 0.000131 0.000093 0.000164 0.000110
Cytoplasm 0.722019* 0.675748* 0.510589* 0.571688* 0.722615* 0.627653* 0.547942* 0.636717* 0.722240* 0.649961* 0.531786* 0.602271*
Cytoplasmic Foci 0.132474 0.165259 0.012220 0.029203 0.124238 0.177338 0.041567 0.032228 0.129424 0.171736 0.028874 0.030626
Eisosomes 0.000075 0.000036 0.000029 0.000031 0.000028 0.000047 0.000066 0.000029 0.000057 0.000042 0.000050 0.000030
Endoplasmic Reticulum 0.000776 0.001224 0.003807 0.001210 0.001349 0.000625 0.001426 0.000644 0.000988 0.000903 0.002456 0.000944
Endosome 0.004692 0.010345 0.002542 0.007343 0.008136 0.017840 0.005915 0.005280 0.005967 0.014364 0.004456 0.006373
Golgi 0.003538 0.004360 0.000197 0.009920 0.002017 0.004527 0.000626 0.000951 0.002975 0.004449 0.000440 0.005702
Lipid Particles 0.001545 0.001048 0.001265 0.000693 0.001667 0.001390 0.001741 0.000821 0.001590 0.001232 0.001535 0.000753
Mitochondria 0.001016 0.001081 0.000430 0.002485 0.001559 0.001354 0.001349 0.001252 0.001217 0.001228 0.000951 0.001905
Mitotic Spindle 0.001482 0.005136 0.006995 0.063319 0.001137 0.004218 0.010003 0.047157 0.001354 0.004644 0.008702 0.055718
None 0.003761 0.000757 0.006767 0.003405 0.005886 0.000681 0.005704 0.003693 0.004548 0.000716 0.006164 0.003541
Nuclear Periphery 0.000069 0.000076 0.000940 0.001086 0.000191 0.000058 0.000857 0.000448 0.000114 0.000066 0.000893 0.000786
Nuclear Periphery Foci 0.000393 0.000873 0.004734 0.002560 0.001328 0.000521 0.002273 0.001759 0.000739 0.000684 0.003338 0.002183
Nucleolus 0.000280 0.000257 0.000301 0.000175 0.000620 0.000462 0.000454 0.000247 0.000406 0.000367 0.000388 0.000209
Nucleus 0.059877 0.019917 0.351374* 0.068548 0.038897 0.013118 0.164250* 0.065251 0.052108 0.016272 0.245181* 0.066998
Peroxisomes 0.000967 0.002515 0.001160 0.001682 0.000897 0.003163 0.000262 0.002616 0.000941 0.002862 0.000650 0.002121
Vacuole 0.051402 0.087118 0.082358 0.152062 0.074948 0.118537 0.205619* 0.162980 0.060121 0.103965 0.152308 0.157197
Vacuole Periphery 0.001086 0.001394 0.000198 0.009811 0.001485 0.001783 0.000778 0.000577 0.001234 0.001602 0.000527 0.005468

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.77 -12.11 -3.31 0.24 0.03 -1.29 -2.27 -1.16 1.00 -0.49 -2.20 -11.65 -3.54 0.71 -0.61
Bud Neck 2.66 0.93 2.62 0.06 1.17 -4.25 -1.11 -0.93 2.78 -0.30 -0.83 1.43 1.80 2.53 0.43
Bud Site -6.62 -0.39 -6.45 -3.16 -5.68 -6.75 2.51 -1.35 3.19 -2.78 -9.62 1.41 -6.22 -0.71 -6.44
Cell Periphery 5.58 -5.06 5.26 -0.54 11.34 0.19 -3.58 4.92 3.73 9.92 4.35 -4.63 7.26 2.53 14.18
Cytoplasm 5.96 25.74 27.49 18.28 -5.09 9.84 22.67 18.55 9.13 -5.91 11.90 34.69 32.91 19.28 -7.28
Cytoplasmic Foci -5.40 39.95 34.98 28.14 -8.02 -8.41 21.55 23.86 32.09 2.85 -9.88 41.54 42.34 42.67 -1.17
Eisosomes 6.74 8.28 10.46 -0.32 1.23 -3.95 -12.44 -3.09 2.77 11.46 4.14 0.58 9.17 1.72 9.72
Endoplasmic Reticulum -1.18 -12.50 -3.04 0.20 11.56 3.51 -4.40 2.64 -2.35 9.82 0.51 -13.29 0.01 -0.56 14.52
Endosome -6.91 5.39 3.92 8.83 -0.61 -7.84 4.38 7.11 13.62 3.46 -11.46 5.27 7.88 16.20 2.79
Golgi -0.76 6.71 1.01 1.50 -2.89 -4.00 5.86 7.43 6.90 0.12 -2.45 7.84 2.67 4.46 -2.71
Lipid Particles 3.81 -1.29 3.29 -1.29 4.73 1.09 -0.87 4.29 3.04 8.82 2.67 -1.76 5.61 2.02 9.46
Mitochondria -0.08 3.88 0.62 0.44 -1.77 0.24 0.94 1.93 1.69 0.59 -0.02 1.33 2.15 1.58 0.03
Mitotic Spindle -2.61 -3.72 -7.79 -6.09 -5.05 -2.36 -4.81 -6.51 -5.11 -0.89 -3.39 -5.82 -10.11 -8.01 -3.65
None 6.95 -2.31 1.30 -8.11 3.89 4.31 0.91 1.66 -8.85 0.61 7.34 -0.33 1.98 -11.92 2.16
Nuclear Periphery -2.26 -19.30 -10.83 -10.65 2.70 2.40 -15.27 -11.11 -20.07 7.29 2.05 -25.41 -15.61 -17.13 6.12
Nuclear Periphery Foci -6.12 -11.08 -7.75 -4.86 7.22 3.52 -4.12 -0.70 -6.81 4.16 0.47 -12.09 -6.61 -7.96 7.97
Nucleolus 0.19 -0.29 0.18 -0.11 0.42 0.65 1.46 2.84 1.55 2.95 0.18 0.67 2.68 1.33 2.74
Nucleus 19.37 -28.10 -12.42 -25.33 23.51 11.67 -21.53 -15.92 -25.33 11.04 23.36 -32.11 -19.11 -35.97 23.54
Peroxisomes -4.96 0.59 1.93 5.72 0.04 -5.71 10.25 0.25 4.44 -1.64 -7.41 2.54 1.06 6.88 -1.19
Vacuole -9.99 -11.36 -34.13 -26.25 -15.83 -9.03 -21.52 -26.05 -20.06 -0.61 -15.13 -24.48 -43.42 -32.56 -9.56
Vacuole Periphery -1.05 5.02 -1.65 -1.36 -2.70 -0.96 3.35 7.96 5.65 0.75 -1.65 4.54 -0.49 0.20 -2.31
Standard name
Human Ortholog
Description F-box protein of unknown function; predicted to be part of an SCF ubiquitin protease complex; involved in regulating protein levels of sulfur metabolism enzymes; may interact with ribosomes, based on co-purification experiments
Localization
Cell Percentages cytoplasm (94%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Skp2

Skp2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Skp2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available