Standard name
Human Ortholog
Description Membrane protein of the plasma membrane and ER; interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane; null mutant requires tryptophan due to mislocalization of tryptophan permease Tat2p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.28 0.25 0.32 0.27 0.14 0.12 0.1 0.1 0.09 0.16 0.18 0.18 0.18 0.15 0.11 0.45 0.25 0.25 0 0.09 0.09 0.08 0.07 0.05
Bud 0.11 0 0.08 0.1 0 0 0 0 0.09 0.06 0.13 0.07 0 0.05 0 0.18 0.05 0.13 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 – – – – – –
Cell Periphery 0.06 0 0.11 0.08 0.06 0.06 0.05 0.05 0.07 0.08 0.11 0.14 0.17 0.09 0.16 0.24 0.25 0.36 0 0 0 0 0 0
Cytoplasm 0.17 0.38 0.08 0.2 0.17 0.22 0.2 0.21 0.35 0.31 0.37 0.3 0.17 0.2 0.13 0.21 0.41 0.39 0 0.09 0 0 0 0
Endoplasmic Reticulum 0.67 0.5 0.67 0.51 0.72 0.6 0.61 0.49 0.3 0.39 0.22 0.35 0.64 0.69 0.75 0 0 0.07 0.79 0.64 0.74 0.72 0.8 0.77
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.12 0 0.09 0 0.13 0.11 0.14 0.21 0.19 0.28 0.22 0 0 0 0.18 0.1 0.05 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.06 0.08 0.06 0.09 0.15 0.11 0.05 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 6 140 109 63 66 71 62 35 79 69 114 64 78 48 17 50 19 2 7 49 33 52 43
Bud 2 1 34 40 11 22 18 25 35 28 50 44 15 25 14 7 11 10 0 2 8 12 12 21
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 0 2 4 17 19 18 16
Bud Site 0 0 1 2 1 2 1 4 3 6 5 6 0 1 0 2 5 0 – – – – – –
Cell Periphery 1 1 49 34 27 32 36 31 27 39 42 88 58 48 74 9 50 27 1 0 21 9 33 31
Cytoplasm 3 9 34 79 76 123 139 134 131 150 139 186 59 102 59 8 82 30 1 7 3 8 14 19
Endoplasmic Reticulum 12 12 296 206 329 337 415 316 110 188 81 217 220 354 341 1 5 5 50 50 389 289 628 626
Endosome 0 0 2 8 6 17 8 4 7 3 4 9 0 0 0 0 5 0 0 0 2 0 1 2
Golgi 0 0 1 6 2 1 1 0 0 1 0 1 0 1 0 0 3 1 0 0 1 2 0 3
Mitochondria 1 3 19 35 12 71 74 90 79 94 106 135 0 0 1 7 20 4 1 0 15 11 10 11
Nucleus 0 0 0 1 0 2 2 7 0 0 1 0 0 2 4 1 3 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 4 3 10 14 6 7 2 6 1 1 0 0 0 0 0 1 1 0 0 2
Nucleolus 0 0 0 0 0 0 3 9 4 1 3 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 3 3 0 0 0 1 0 0 1 0 0 0 0 0 0 1 0 0 2 0 0 0
SpindlePole 0 0 0 3 1 1 0 0 0 0 0 2 1 0 3 1 1 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 18 26 37 33 61 93 41 25 20 26 2 16 14 1 9 4 0 2 7 10 15 25
Unique Cell Count 18 24 443 405 457 564 682 641 370 484 374 627 346 515 455 38 202 76 64 78 523 400 790 811
Labelled Cell Count 24 32 597 553 569 710 839 790 478 621 523 836 421 629 558 54 245 102 64 78 523 400 790 811


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.5 10.2 9.9 10.0 10.0 8.9 8.7 8.9 8.5 8.2 8.0 8.3 11.3 11.5 12.6 8.2 9.7 12.2 12.6 12.9 12.2
Std Deviation (1e-4) 2.0 2.3 2.0 1.9 1.9 1.7 1.7 1.7 1.7 1.6 1.6 1.6 1.8 2.0 2.4 1.8 2.3 6.1 2.2 2.3 2.2
Intensity Change (Log2) – – – 0.01 0.01 -0.15 -0.19 -0.16 -0.22 -0.28 -0.3 -0.26 0.18 0.22 0.35 -0.27 -0.03 0.3 0.34 0.38 0.3

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP700051015WT3HU80HU120HU160051015WT3rpd3Δ_1rpd3Δ_2rpd3Δ_3051015WT1AF100AF140AF180051015
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.5 -6.4 -7.8 -8.9 -9.1 -7.6 -5.5 -4.3 -5.1 -4.2 -6.1 -7.8 1.7 -1.8 -1.2
Bud 1.1 -3.6 -2.6 -3.9 -2.7 0.9 -1.2 2.7 -0.4 -1.9 -1.8 -3.1 0 -1.0 1.6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.3 -2.8 -3.1 -3.6 -3.9 -1.8 -1.6 0.1 1.4 2.3 -0.9 2.3 0 4.5 5.6
Cytoplasm 5.1 4.1 6.1 5.8 5.9 9.8 8.9 10.3 8.8 4.1 5.4 2.6 0 10.1 7.8
Endoplasmic Reticulum -4.7 1.7 -2.3 -2.0 -5.7 -10.5 -8.5 -12.9 -10.4 -0.9 0.6 2.7 -7.8 -15.2 -9.8
Endosome 0 0 3.0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.6 -1.4 4.6 3.9 5.2 7.4 7.0 9.5 7.9 -3.9 -4.7 -4.1 0 2.8 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 3.1 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.5 2.5 1.3 3.1 5.6 3.8 0.8 0.9 0.1 -3.1 -0.8 -0.8 0 0.2 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.2955 5.1644 6.0386 5.4542 5.1218 5.5069 4.9254 4.2826 5.2766 4.2914 4.8962 5.2499 3.8267 4.8739 4.6715 4.4067 3.955 5.5715
Actin 0.0734 0.0354 0.0597 0.0485 0.0172 0.0429 0.0317 0.0818 0.1042 0.1341 0.0698 0.0703 0.063 0.0295 0.057 0.1156 0.1274 0.0305
Bud 0.0098 0.0246 0.0871 0.0448 0.0167 0.009 0.0114 0.0022 0.0503 0.0108 0.0131 0.0023 0.003 0.0018 0.0298 0.0144 0.0045 0.0013
Bud Neck 0.0343 0.0113 0.0138 0.0068 0.077 0.2911 0.0184 0.0071 0.0116 0.0307 0.0037 0.283 0.019 0.0114 0.0219 0.0049 0.0043 0.4779
Bud Periphery 0.0455 0.0559 0.2865 0.2636 0.1238 0.0427 0.0363 0.0042 0.1864 0.1216 0.1449 0.0094 0.0111 0.0035 0.1329 0.1018 0.0408 0.0119
Bud Site 0.074 0.1471 0.0799 0.0296 0.0068 0.0047 0.0322 0.1219 0.046 0.0053 0.002 0.0062 0.0797 0.2011 0.0321 0.02 0.0032 0.0013
Cell Periphery 0.0723 0.033 0.0286 0.0373 0.0252 0.0266 0.0461 0.018 0.0258 0.0535 0.0396 0.0249 0.0518 0.059 0.0401 0.0536 0.036 0.028
Cytoplasm 0.2255 0.3308 0.1443 0.1889 0.1551 0.2172 0.1991 0.2876 0.1404 0.281 0.2747 0.1619 0.2603 0.2782 0.2026 0.2021 0.4024 0.1669
Cytoplasmic Foci 0.0315 0.0106 0.0075 0.0297 0.0095 0.0081 0.0423 0.0123 0.0167 0.0156 0.0128 0.0121 0.0117 0.0076 0.013 0.0042 0.0427 0.0035
Eisosomes 0.0014 0.0009 0.0008 0.001 0.0013 0.0006 0.0012 0.0011 0.0008 0.0015 0.0011 0.0005 0.0025 0.0004 0.0007 0.0009 0.0014 0.0005
Endoplasmic Reticulum 0.2708 0.2017 0.1675 0.1781 0.3792 0.262 0.4192 0.3245 0.3002 0.2209 0.304 0.3622 0.297 0.2502 0.3081 0.3359 0.1531 0.2421
Endosome 0.0383 0.0259 0.0137 0.0132 0.0419 0.015 0.0184 0.0139 0.0227 0.0154 0.0217 0.0126 0.0164 0.0121 0.0181 0.0092 0.0777 0.0015
Golgi 0.0161 0.0074 0.0064 0.0076 0.0161 0.0067 0.0152 0.0086 0.0215 0.0208 0.0081 0.013 0.0191 0.0053 0.0192 0.0116 0.0265 0.0019
Lipid Particles 0.0244 0.0096 0.0042 0.013 0.0092 0.0053 0.034 0.0211 0.0233 0.0075 0.0117 0.0153 0.0204 0.0323 0.0167 0.0058 0.0315 0.0019
Mitochondria 0.0069 0.002 0.0078 0.0034 0.0017 0.002 0.0042 0.0006 0.0054 0.0042 0.0035 0.0058 0.0069 0.0007 0.0088 0.0031 0.0144 0.0002
None 0.0092 0.0681 0.054 0.0651 0.084 0.0407 0.0573 0.0682 0.018 0.0559 0.071 0.0069 0.1011 0.0731 0.0581 0.0918 0.0033 0.02
Nuclear Periphery 0.0232 0.0106 0.0111 0.0069 0.0068 0.0088 0.0078 0.0105 0.0098 0.0075 0.0075 0.0037 0.0147 0.0129 0.0121 0.0133 0.0081 0.0035
Nucleolus 0.003 0.0008 0.0009 0.0005 0.0003 0.0003 0.0009 0.0003 0.0002 0.0002 0.0001 0.0002 0.0005 0.0003 0.0004 0.0002 0.0017 0.0001
Nucleus 0.0054 0.0061 0.0044 0.0021 0.0019 0.0044 0.0018 0.0022 0.0019 0.0015 0.0016 0.0015 0.0022 0.0029 0.0038 0.0023 0.0038 0.0013
Peroxisomes 0.0034 0.0004 0.0062 0.0396 0.0002 0.0005 0.0077 0.0003 0.0008 0.0007 0.0003 0.0017 0.0004 0.0002 0.0033 0.0002 0.0021 0.0001
Punctate Nuclear 0.009 0.0013 0.0014 0.0069 0.0006 0.001 0.0019 0.0015 0.0007 0.0005 0.0013 0.0007 0.0008 0.0013 0.0009 0.0005 0.0016 0.0002
Vacuole 0.0202 0.0151 0.012 0.0116 0.0237 0.009 0.0121 0.011 0.0115 0.0092 0.0065 0.0047 0.0157 0.0155 0.0183 0.0065 0.0109 0.0049
Vacuole Periphery 0.0021 0.0013 0.002 0.0017 0.0021 0.0013 0.0011 0.0011 0.0017 0.0015 0.0009 0.0009 0.0027 0.0007 0.0021 0.0022 0.0027 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 42.5409 45.1594 37.0376 34.1983 32.749 38.0885 36.8489 41.0904 47.4133 42.7364
Translational Efficiency 1.6192 1.1649 1.3467 1.3787 1.3989 1.6747 1.5507 1.3471 1.1485 1.1677

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
86 197 311 773 1151 1333 224 1173 1237 1530 535 1946

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1218.52 1118.18 1480.31 1221.62 895.32 1075.33 1399.54 1319.71 917.79 1080.85 1446.49 1280.75
Standard Deviation 162.09 220.38 205.13 255.32 163.62 191.23 205.64 233.38 183.01 195.76 209.17 247.04
Intensity Change Log 2 — -0.123977 0.280769 0.003666 — 0.264304 0.644477 0.559746 — 0.053375 0.446127 0.265718

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.006164 0.008347 0.012390 0.011388 0.005073 0.005829 0.006688 0.010809 0.005149 0.006153 0.010002 0.011039
Bud Neck 0.009404 0.019782 0.034385 0.016051 0.016685 0.037497 0.015808 0.017035 0.016179 0.035216 0.026607 0.016644
Bud Site 0.132486 0.143416 0.085987 0.145531 0.080179 0.119702 0.056101 0.133955 0.083816 0.122756 0.073474 0.138553
Cell Periphery 0.039285 0.017431 0.040430 0.103774 0.055883 0.046671 0.035514 0.078846 0.054729 0.042906 0.038372 0.088748
Cytoplasm 0.052515 0.090491 0.014528 0.143953 0.074459 0.098719 0.020051 0.105942 0.072933 0.097660 0.016841 0.121041
Cytoplasmic Foci 0.012925 0.046270 0.007275 0.019519 0.021531 0.015455 0.006278 0.013808 0.020933 0.019423 0.006858 0.016077
Eisosomes 0.000992 0.000462 0.000424 0.001427 0.001271 0.001171 0.000292 0.000862 0.001252 0.001080 0.000369 0.001087
Endoplasmic Reticulum 0.474577 0.547900 0.681294 0.341119 0.676085 0.603700 0.767330 0.476639 0.662076 0.596515 0.717317 0.422807
Endosome 0.016247 0.027742 0.020610 0.013302 0.010024 0.012265 0.011741 0.013670 0.010456 0.014257 0.016896 0.013524
Golgi 0.012320 0.007676 0.006083 0.009277 0.006058 0.006249 0.004205 0.007190 0.006493 0.006433 0.005296 0.008019
Lipid Particles 0.013702 0.024207 0.028616 0.101828 0.011408 0.012461 0.019511 0.065984 0.011567 0.013973 0.024804 0.080222
Mitochondria 0.004604 0.004492 0.000233 0.001532 0.011074 0.005855 0.000200 0.000986 0.010624 0.005680 0.000219 0.001203
Mitotic Spindle 0.000381 0.001084 0.001015 0.001139 0.000237 0.000296 0.000301 0.000556 0.000247 0.000398 0.000716 0.000788
None 0.000013 0.000293 0.000038 0.000177 0.002382 0.000774 0.000140 0.000603 0.002217 0.000712 0.000081 0.000434
Nuclear Periphery 0.000586 0.000240 0.000593 0.000509 0.000217 0.000203 0.000347 0.000368 0.000243 0.000208 0.000490 0.000424
Nuclear Periphery Foci 0.007577 0.004469 0.011920 0.007228 0.001762 0.001618 0.008623 0.004146 0.002166 0.001985 0.010540 0.005370
Nucleolus 0.000031 0.000059 0.000029 0.000065 0.000094 0.000106 0.000034 0.000068 0.000089 0.000100 0.000031 0.000067
Nucleus 0.001614 0.004892 0.000659 0.004471 0.001676 0.002570 0.002063 0.003261 0.001672 0.002869 0.001247 0.003741
Peroxisomes 0.000815 0.002532 0.000325 0.002726 0.013808 0.007833 0.000406 0.003572 0.012905 0.007151 0.000359 0.003236
Vacuole 0.211827 0.042727 0.051005 0.070877 0.006355 0.018114 0.042819 0.058195 0.020640 0.021284 0.047578 0.063233
Vacuole Periphery 0.001934 0.005486 0.002163 0.004106 0.003739 0.002911 0.001546 0.003505 0.003614 0.003242 0.001905 0.003744

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.89 -1.89 -2.62 -1.48 0.24 -0.72 -0.75 -4.25 -4.10 -1.38 -1.02 -2.37 -5.35 -5.07 -0.48
Bud Neck -1.92 -4.63 -2.53 0.72 3.62 -5.26 0.16 -0.12 5.60 -0.24 -5.32 -2.76 -0.19 5.88 2.94
Bud Site -0.29 1.45 -0.46 -0.20 -4.50 -4.94 2.07 -6.21 -1.59 -6.32 -4.96 1.05 -7.41 -2.12 -7.11
Cell Periphery 1.72 -0.14 -4.80 -11.79 -7.56 2.14 3.28 -4.06 -6.34 -6.36 2.92 3.26 -6.77 -10.58 -9.48
Cytoplasm -2.65 4.07 -8.41 -3.94 -15.88 -3.86 9.24 -4.95 -1.36 -12.95 -4.23 11.68 -8.63 -4.25 -20.51
Cytoplasmic Foci -3.78 1.26 -1.36 3.38 -4.23 2.75 5.11 3.42 0.88 -2.70 0.57 6.03 2.35 1.80 -4.69
Eisosomes 1.67 1.79 -1.20 -4.18 -4.36 0.45 4.96 1.94 2.00 -5.01 0.83 4.83 0.83 -0.01 -6.22
Endoplasmic Reticulum -1.45 -4.33 2.89 6.49 14.10 4.99 -3.57 13.50 8.67 11.89 4.68 -2.77 18.45 13.73 16.97
Endosome -2.65 -1.50 1.04 3.97 3.89 -2.13 -1.22 -3.51 -1.29 -1.19 -3.55 -4.96 -3.42 0.71 2.71
Golgi 0.53 0.72 0.35 -0.84 -1.77 -0.19 1.99 -1.05 -0.81 -2.83 0.04 1.07 -1.34 -1.55 -2.61
Lipid Particles -1.55 -2.38 -11.11 -9.37 -9.39 -0.82 -2.26 -13.87 -13.47 -8.90 -1.78 -4.40 -19.37 -18.31 -12.14
Mitochondria 0.01 0.99 0.68 1.18 -2.19 3.64 9.16 8.47 5.98 -6.44 3.58 9.08 8.05 5.57 -4.01
Mitotic Spindle -1.42 -0.99 -2.15 -0.12 -0.13 -0.85 -0.91 -3.54 -2.61 -2.44 -1.74 -1.27 -3.91 -2.55 -0.14
None -2.46 -1.99 -6.28 0.99 -5.00 1.81 2.56 2.00 0.61 -2.12 1.83 2.63 2.18 1.34 -2.85
Nuclear Periphery 2.00 -0.12 0.47 -3.80 0.71 0.77 -3.37 -5.15 -5.85 -0.31 1.63 -3.04 -5.81 -7.65 0.93
Nuclear Periphery Foci 1.45 -1.78 0.19 -1.79 2.35 0.44 -3.41 -5.96 -9.60 2.22 0.49 -5.83 -7.08 -9.09 3.62
Nucleolus -1.85 0.13 -2.92 -0.33 -4.43 -1.04 3.91 2.36 4.19 -2.54 -1.00 5.52 2.29 4.27 -4.98
Nucleus -1.91 2.22 -4.52 -0.31 -6.07 -2.76 -0.97 -2.96 -1.38 -1.28 -3.33 0.82 -5.36 -2.51 -5.32
Peroxisomes -3.02 2.44 -3.76 -0.15 -5.11 4.11 10.52 7.51 4.92 -5.95 4.30 10.58 7.85 5.52 -7.80
Vacuole 5.28 5.00 4.46 -3.91 -2.60 -7.89 -5.27 -15.89 -11.84 -1.94 -0.10 -5.11 -11.66 -15.12 -2.84
Vacuole Periphery -1.82 -0.28 -1.65 0.69 -1.73 1.77 4.70 0.36 -0.86 -2.86 0.78 3.95 -0.16 -0.74 -2.97
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Membrane protein of the plasma membrane and ER; interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane; null mutant requires tryptophan due to mislocalization of tryptophan permease Tat2p
Localization
Cell Percentages ER (93%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Lem3

Lem3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lem3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available