Standard name
Human Ortholog
Description Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain; homologous to human FIG4, which is associated with CMT4J, a form of Charcot-Marie-Tooth disorder
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.05 0 0 0 0 0 0.05 – 0.08 0 0 0 0 0 0 0.12 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.53 0.57 0.79 0.86 0.82 0.83 0.88 0.88 0.74 – 0.69 0.86 0.68 0.69 0.73 0.55 0.78 0.25 0.13 0.15 0.22 0.1 0.1 0.09
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0
Endosome 0.17 0.05 0.05 0 0.05 0 0 0 0 – 0 0 0.12 0.14 0.12 0.15 0.06 0.38 0.33 0.2 0.19 0.2 0.19 0.15
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.2 0.14 0 0 0 0.12 0.11 0.07 0.32 – 0.47 0.19 0 0 0 0.07 0 0.06 0.12 0.09 0.16 0.07 0.07 0.06
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0.17 0.33 0.11 0.06 0.08 0.05 0 0 0.06 – 0.07 0 0.25 0.34 0.19 0.25 0.17 0.56 0.31 0.5 0.32 0.53 0.53 0.57
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 6 0 0 0 0 2 0 – 0 1 0 0 0 1 0 0 1 1 3 0 1 3
Bud 4 2 11 2 5 4 1 7 13 – 15 8 3 3 7 2 0 2 1 1 1 5 6 8
Bud Neck 0 0 10 1 1 1 7 12 3 – 4 5 0 0 0 0 0 0 0 1 1 0 2 2
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 1 1 1 1 – 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 85 101 179 138 187 240 257 356 193 – 136 250 172 286 351 30 14 4 29 32 64 22 31 30
Endoplasmic Reticulum 6 6 2 4 1 1 2 0 0 – 1 1 1 0 0 3 1 0 5 1 4 6 7 11
Endosome 27 8 12 3 11 11 1 4 1 – 2 3 30 58 57 8 1 6 77 44 54 46 59 50
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 2 0 2 0 2 3
Mitochondria 32 25 5 4 3 35 31 30 83 – 92 55 9 8 8 4 0 1 27 20 44 15 21 19
Nucleus 0 0 0 1 1 3 6 5 4 – 1 4 0 1 2 0 1 1 1 1 2 1 1 0
Nuclear Periphery 3 2 0 0 0 0 0 0 0 – 0 0 0 1 1 0 0 0 2 0 3 2 3 4
Nucleolus 2 0 1 0 0 0 0 0 0 – 0 0 0 0 2 0 0 0 1 0 0 1 1 0
Peroxisomes 0 0 1 0 0 0 0 0 0 – 0 0 1 3 3 0 0 0 1 0 0 0 0 0
SpindlePole 7 0 7 4 11 6 4 15 3 – 1 5 1 1 7 2 0 1 5 2 5 1 4 2
Vac/Vac Membrane 27 59 24 9 19 13 12 11 16 – 13 7 63 142 90 14 3 9 72 113 92 122 168 195
Unique Cell Count 161 177 227 161 227 288 293 405 262 197 292 252 414 481 55 18 16 232 225 288 232 316 340
Labelled Cell Count 195 203 258 166 239 315 322 443 317 266 341 280 503 528 65 20 24 232 225 288 232 316 340


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 4.5 5.2 4.6 5.1 4.1 4.0 4.4 3.7 – 3.5 3.9 4.8 4.9 4.7 7.3 6.3 6.6 4.5 4.8 5.4
Std Deviation (1e-4) 0.8 0.6 1.2 1.1 1.6 1.4 1.1 1.1 1.1 – 1.1 1.3 1.1 1.5 1.3 2.3 0.9 1.1 1.6 2.1 3.5
Intensity Change (Log2) – – – -0.17 -0.01 -0.35 -0.37 -0.25 -0.49 – -0.57 -0.39 -0.1 -0.07 -0.13 0.49 0.29 0.35 -0.21 -0.11 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 – –
Bud Neck – – – – – – – – – – – – 0 – –
Bud Site – – – – – – – – – – – – 0 – –
Cell Periphery – – – – – – – – – – – – 0 – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 – –
Endosome – – – – – – – – – – – – 0 – –
Golgi – – – – – – – – – – – – 0 – –
Mitochondria – – – – – – – – – – – – 0 – –
Nuclear Periphery – – – – – – – – – – – – 0 – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 – –
Peroxisomes – – – – – – – – – – – – 0 – –
SpindlePole – – – – – – – – – – – – 0 – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 – –
Cytoplasm – – – – – – – – – – – – 0 – –
Nucleus – – – – – – – – – – – – 0 – –
Vacuole – – – – – – – – – – – – 2.9 – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9482 1.6698 1.5608 1.7358 1.5977 1.6034 -0.2742 -0.3506 -0.1884 -0.4524 -0.5859 -0.4463 -0.1847 0.3073 0.0304 0.2259 -0.0543 -0.063
Actin 0.0154 0.0007 0.0086 0.0004 0.0213 0.0042 0.0081 0.0003 0.0039 0.0034 0 0.0032 0.0049 0.0011 0.0027 0.0027 0.0036 0.0012
Bud 0.0007 0.0006 0.0004 0.0065 0.0008 0.0004 0.0054 0.0013 0.0072 0.0047 0.0003 0.0037 0.0174 0.0399 0.0203 0.0408 0.0086 0.0186
Bud Neck 0.0043 0.0002 0.0024 0.0114 0.001 0.0018 0.0012 0.0004 0.0013 0.0009 0.0006 0.002 0.0022 0.0028 0.0024 0.0069 0.0022 0.0042
Bud Periphery 0.0027 0.0024 0.0008 0.0213 0.0012 0.0008 0.0024 0.0009 0.0076 0.0125 0.0004 0.0036 0.0129 0.0195 0.0154 0.0527 0.0119 0.0148
Bud Site 0.0044 0.0016 0.0022 0.0076 0.0011 0.0004 0.0042 0.017 0.0111 0.0027 0.0004 0.0009 0.0222 0.1101 0.0397 0.0249 0.0026 0.0023
Cell Periphery 0.0015 0.0013 0.0008 0.0009 0.0004 0.0003 0.0003 0.0002 0.0006 0.0004 0.0001 0.0002 0.0015 0.0018 0.0013 0.0021 0.0006 0.0008
Cytoplasm 0.1113 0.2768 0.1587 0.0881 0.1263 0.2378 0.179 0.3622 0.2301 0.2374 0.2888 0.3488 0.1888 0.2249 0.1694 0.148 0.2047 0.2438
Cytoplasmic Foci 0.0441 0.0804 0.107 0.1221 0.064 0.0788 0.106 0.0808 0.1208 0.1554 0.1146 0.1162 0.1422 0.1134 0.1248 0.1121 0.1059 0.1323
Eisosomes 0.0027 0.0008 0.0041 0.0002 0.0006 0.0002 0.0001 0 0.0001 0.0001 0 0 0.0003 0.0002 0.0002 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0047 0.0047 0.0049 0.0025 0.0029 0.0034 0.0037 0.0042 0.0051 0.0032 0.0028 0.0028 0.0064 0.0031 0.0045 0.0051 0.0048 0.0029
Endosome 0.1286 0.0788 0.157 0.1831 0.0828 0.1429 0.2668 0.1733 0.2352 0.2768 0.1737 0.2012 0.1108 0.1076 0.1454 0.1387 0.1462 0.0872
Golgi 0.0062 0.0026 0.0055 0.006 0.0134 0.0059 0.0169 0.004 0.0128 0.0217 0.0017 0.0078 0.0044 0.0024 0.0055 0.0063 0.0025 0.0015
Lipid Particles 0.0121 0.0051 0.0127 0.0117 0.0252 0.0121 0.0218 0.0013 0.0125 0.0126 0.001 0.0036 0.0095 0.0037 0.0048 0.0072 0.0051 0.0043
Mitochondria 0.0096 0.0081 0.0233 0.0154 0.0239 0.0161 0.0208 0.0114 0.012 0.0261 0.0023 0.0088 0.0067 0.0033 0.01 0.0102 0.0046 0.0031
None 0.5791 0.4925 0.4291 0.4398 0.5065 0.4417 0.2459 0.2751 0.2151 0.1317 0.3198 0.2386 0.3706 0.2767 0.3544 0.3416 0.3788 0.3969
Nuclear Periphery 0.004 0.0023 0.0032 0.0016 0.0036 0.0019 0.0073 0.0028 0.0052 0.0023 0.0025 0.0024 0.0078 0.0026 0.0049 0.0051 0.0074 0.0035
Nucleolus 0.0017 0.0013 0.003 0.0045 0.0014 0.0009 0.0013 0.0006 0.0049 0.0016 0.0006 0.0006 0.0058 0.0054 0.0057 0.006 0.0036 0.003
Nucleus 0.0027 0.0032 0.0025 0.0028 0.0016 0.015 0.0033 0.0035 0.008 0.0035 0.0033 0.0046 0.012 0.0174 0.0169 0.0123 0.0139 0.0124
Peroxisomes 0.0085 0.0026 0.0132 0.009 0.0366 0.0035 0.0245 0.0026 0.0146 0.0105 0.0006 0.0077 0.0046 0.0018 0.0039 0.0026 0.0028 0.0018
Punctate Nuclear 0.002 0.0013 0.0064 0.0019 0.0356 0.0033 0.0045 0.0012 0.0097 0.0026 0.0019 0.003 0.0201 0.0193 0.027 0.0079 0.0309 0.0386
Vacuole 0.046 0.0292 0.0471 0.0548 0.0406 0.0249 0.0687 0.05 0.0699 0.0669 0.0746 0.033 0.0448 0.0375 0.0351 0.0495 0.0526 0.0229
Vacuole Periphery 0.0082 0.0034 0.0071 0.0084 0.0094 0.0037 0.0079 0.0067 0.0124 0.023 0.01 0.0072 0.0041 0.0056 0.0056 0.0172 0.0065 0.0038

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.0604 4.8572 5.8222 8.8805 6.2318 7.9368 7.5555 7.7767 8.7842 10.2215
Translational Efficiency 0.6676 0.5572 0.7035 0.4822 0.5798 0.5721 0.5716 0.406 0.5179 0.483

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2019 625 201 1568 1631 1951 1458 703 3650 2576 1659 2271

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 650.06 770.51 989.97 838.95 665.52 740.82 868.74 827.08 656.97 748.02 883.43 835.28
Standard Deviation 63.66 97.36 121.03 111.34 113.46 105.73 99.23 114.38 89.74 104.54 109.52 112.43
Intensity Change Log 2 — 0.245241 0.606812 0.368012 — 0.154641 0.384442 0.313545 — 0.200120 0.498603 0.340715

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000178 0.000413 0.000979 0.000407 0.000191 0.000193 0.000182 0.000316 0.000184 0.000246 0.000279 0.000378
Bud Neck 0.054861 0.024673 0.017655 0.025986 0.031682 0.027265 0.022577 0.021752 0.044503 0.026637 0.021981 0.024675
Bud Site 0.011547 0.014065 0.031612 0.041886 0.009488 0.016566 0.014100 0.038699 0.010627 0.015959 0.016222 0.040899
Cell Periphery 0.000127 0.000164 0.000183 0.000062 0.000114 0.000220 0.000066 0.000081 0.000121 0.000206 0.000080 0.000068
Cytoplasm 0.154529* 0.108003* 0.123588* 0.243105* 0.270390* 0.108401* 0.274954* 0.215920* 0.206301* 0.108304* 0.256615* 0.234689*
Cytoplasmic Foci 0.286374* 0.245128* 0.059675 0.088705 0.265711* 0.198636* 0.035757 0.109237 0.277141* 0.209916* 0.038655 0.095061
Eisosomes 0.000241 0.000138 0.000103 0.000024 0.000168 0.000094 0.000041 0.000035 0.000208 0.000105 0.000049 0.000027
Endoplasmic Reticulum 0.002261 0.002336 0.012499 0.001317 0.002056 0.003423 0.003735 0.001673 0.002170 0.003159 0.004797 0.001428
Endosome 0.221873* 0.282836* 0.076671 0.105054 0.125559 0.260294* 0.060788 0.128233 0.178835 0.265763* 0.062713 0.112229
Golgi 0.025150 0.017771 0.001257 0.003467 0.007378 0.015924 0.000847 0.003972 0.017209 0.016372 0.000896 0.003624
Lipid Particles 0.026448 0.012087 0.011878 0.002440 0.022894 0.020926 0.003326 0.004123 0.024860 0.018781 0.004363 0.002961
Mitochondria 0.028989 0.020426 0.000995 0.002310 0.009554 0.033858 0.001669 0.002003 0.020304 0.030599 0.001587 0.002215
Mitotic Spindle 0.001855 0.002563 0.035536 0.095512 0.001547 0.009854 0.011283 0.070231 0.001717 0.008085 0.014222 0.087686
None 0.001370 0.000697 0.000849 0.000894 0.007068 0.001390 0.002534 0.001235 0.003917 0.001222 0.002329 0.001000
Nuclear Periphery 0.000346 0.000550 0.006011 0.001429 0.000837 0.001187 0.004765 0.001683 0.000565 0.001032 0.004916 0.001508
Nuclear Periphery Foci 0.000480 0.000434 0.026006 0.002504 0.000848 0.000778 0.004342 0.004658 0.000644 0.000694 0.006967 0.003170
Nucleolus 0.002133 0.000861 0.009562 0.003234 0.002669 0.003109 0.014032 0.005442 0.002372 0.002564 0.013491 0.003917
Nucleus 0.019959 0.008791 0.159160* 0.030633 0.053354 0.016350 0.207945* 0.035998 0.034881 0.014516 0.202034* 0.032294
Peroxisomes 0.016531 0.012820 0.009607 0.013487 0.010115 0.009022 0.005794 0.010116 0.013664 0.009943 0.006256 0.012443
Vacuole 0.134318 0.220258* 0.409962* 0.330016* 0.171523 0.242929* 0.326434* 0.335895* 0.150943 0.237428* 0.336554* 0.331836*
Vacuole Periphery 0.010428 0.024987 0.006211 0.007530 0.006855 0.029582 0.004828 0.008697 0.008832 0.028467 0.004995 0.007891

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.33 -6.99 -3.92 0.34 5.41 -0.01 -0.63 -2.85 -3.07 -2.90 -1.16 -4.27 -4.57 -1.83 -0.77
Bud Neck 8.80 9.37 10.09 0.01 -1.76 1.65 2.91 4.93 3.76 2.24 8.20 9.25 10.59 2.25 0.30
Bud Site -1.24 -2.36 -8.73 -6.00 -0.70 -4.54 -2.51 -7.14 -4.87 -5.49 -4.49 -2.98 -11.34 -7.73 -7.03
Cell Periphery -1.45 -3.15 8.36 4.63 8.04 -1.78 5.54 3.61 2.42 -1.17 -1.87 6.05 9.22 3.23 3.86
Cytoplasm 5.85 2.32 -12.26 -15.67 -9.25 21.40 -0.42 4.12 -10.88 4.38 18.70 -6.77 -5.59 -20.84 1.46
Cytoplasmic Foci 4.52 30.87 38.44 20.45 -3.80 10.38 45.14 23.22 14.66 -13.00 13.33 64.25 45.73 28.14 -16.16
Eisosomes 3.68 5.27 8.95 7.66 9.22 5.69 10.11 10.51 11.05 1.97 6.85 10.87 12.40 14.62 9.83
Endoplasmic Reticulum -0.19 -10.19 3.35 3.12 11.31 -3.42 -8.15 1.44 4.32 10.58 -3.02 -11.17 3.92 5.67 16.43
Endosome -5.59 18.30 24.46 20.83 -0.77 -19.73 14.50 3.68 20.88 -7.79 -14.86 28.74 21.60 33.77 -7.12
Golgi 3.27 18.45 17.48 8.21 -2.14 -8.28 12.06 4.57 10.76 -2.45 0.75 21.49 17.79 15.91 -4.10
Lipid Particles 7.88 8.78 18.01 6.87 6.01 0.94 14.02 12.99 11.92 -3.17 4.28 21.23 22.39 14.17 2.77
Mitochondria 2.69 16.88 16.28 6.75 -2.97 -11.65 10.17 9.90 16.59 0.05 -5.41 18.83 18.48 17.81 -1.02
Mitotic Spindle -0.71 -4.41 -19.12 -18.82 -7.91 -5.69 -6.81 -11.42 -10.37 -9.67 -5.72 -8.10 -22.26 -20.65 -17.88
None 1.42 1.87 0.06 -1.58 -2.88 3.89 3.27 3.88 -0.00 2.36 3.79 2.52 3.80 -0.39 3.32
Nuclear Periphery -1.30 -8.07 -15.30 -10.15 5.10 -1.35 -14.89 -6.87 -4.00 8.33 -2.46 -18.12 -15.59 -7.32 9.94
Nuclear Periphery Foci 0.54 -6.08 -9.67 -9.83 5.09 0.22 -6.53 -6.68 -6.67 -2.50 -0.38 -8.95 -11.12 -10.64 2.15
Nucleolus 3.66 -2.92 -6.11 -8.93 1.53 -0.72 -8.02 -3.40 -2.89 4.19 -0.43 -8.75 -6.83 -5.70 5.43
Nucleus 7.78 -8.97 -9.70 -14.91 7.32 13.69 -23.58 3.13 -7.08 24.28 12.85 -28.78 -3.63 -13.95 26.71
Peroxisomes 2.12 3.42 4.83 1.74 -0.47 1.29 4.62 0.77 -0.10 -2.78 4.53 8.44 4.10 0.46 -3.48
Vacuole -9.04 -14.57 -24.88 -10.21 4.35 -9.86 -18.80 -13.92 -7.54 0.01 -15.59 -26.02 -27.74 -12.92 1.05
Vacuole Periphery -6.76 4.71 8.01 9.45 0.24 -18.59 5.87 1.31 17.99 -2.79 -18.61 11.59 7.12 21.31 -1.58
Standard name
Human Ortholog
Description Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain; homologous to human FIG4, which is associated with CMT4J, a form of Charcot-Marie-Tooth disorder
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Fig4

Fig4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fig4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available