Standard name
Human Ortholog
Description Palmitoyltransferase for Vac8p; required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.13 0.08 0.14 0.26 0.37 0.58 0.57 0.61 0.63 0.67 0.62 0.64 0.12 0.18 0.16 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0.06 0 0 0.07 0.06 0 0 0 0 0 0 0 0.08 0.1 0.19 – – – 0.05 0.07 0.07 0.06 0.09 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.06 0 0.32 0.21 0.2 0.27 0.33 0.23 0.26 0.22 0.33 0.3 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0.07 0.1 0 0 0 0 0 0 0 0 0 0 0.1 0 0 – – – 0.05 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.81 0.88 0.68 0.73 0.6 0.32 0.34 0.27 0.33 0.3 0.33 0.28 0.83 0.79 0.83 – – – 0.84 0.85 0.82 0.84 0.79 0.81
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 1 1 6 20 20 19 22 13 9 0 1 1 – – – 1 2 0 2 2 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 1 0 1 2 1 1 0 0 1 – – – 0 0 0 0 0 0
Cytoplasm 21 28 33 94 242 424 519 633 521 631 380 585 22 62 87 – – – 1 5 5 4 7 9
Endoplasmic Reticulum 0 0 2 1 0 7 3 2 1 0 0 5 3 1 2 – – – 1 0 1 0 1 3
Endosome 10 11 10 27 40 2 6 21 19 17 1 2 14 35 105 – – – 8 24 15 19 31 40
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 – – – 0 2 0 0 0 0
Mitochondria 9 10 74 76 131 194 298 237 217 206 202 272 7 2 1 – – – 1 2 7 6 5 4
Nucleus 11 34 8 3 14 20 16 31 13 11 12 14 17 13 15 – – – 8 12 3 7 5 5
Nuclear Periphery 3 4 4 15 1 7 13 16 2 8 0 0 0 2 2 – – – 1 2 4 4 5 3
Nucleolus 5 2 7 4 2 0 1 3 0 1 0 0 0 0 3 – – – 0 0 0 0 1 1
Peroxisomes 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 1 0
SpindlePole 1 0 0 1 2 1 0 1 1 0 0 0 1 0 3 – – – 0 0 0 1 2 3
Vac/Vac Membrane 129 299 156 263 396 234 312 277 268 286 200 258 147 271 459 – – – 143 301 187 254 267 355
Unique Cell Count 159 338 228 362 659 729 910 1032 821 939 613 910 178 341 553 170 356 228 303 337 441
Labelled Cell Count 189 388 294 486 829 896 1189 1242 1062 1184 809 1146 211 387 680 170 356 228 303 337 441


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 7.1 4.9 4.7 4.5 4.1 3.8 4.2 4.1 4.0 4.0 4.1 6.4 6.2 6.7 – – – 7.2 7.3 7.3
Std Deviation (1e-4) 1.3 1.4 1.2 1.4 1.3 1.3 1.1 1.2 1.2 0.9 1.1 1.1 1.0 1.3 1.1 – – – 1.4 1.5 1.5
Intensity Change (Log2) – – – -0.07 -0.12 -0.25 -0.37 -0.21 -0.24 -0.29 -0.28 -0.26 0.37 0.33 0.44 – – – 0.55 0.56 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cytoplasm 3.3 6.3 11.5 11.5 12.8 13.1 14.4 12.3 13.5 -0.6 1.2 0.4 – – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Endosome 0 0 0 0 0 0 0 0 0 1.5 2.5 5.2 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 0 0 0 0 0 0 0 0 0 -7.1 -11.0 -13.9 – – –
Nucleus 0 -1.2 -0.6 0 -0.4 0 0 -1.3 0 2.5 0.2 -0.6 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole 1.1 -2.2 -9.7 -9.4 -12.0 -9.7 -10.6 -9.3 -11.2 3.3 3.0 4.5 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5879 1.1512 0.9274 0.5386 0.9361 0.8234 0.7439 1.694 1.6296 1.8995 0.6784 1.5728 -1.1541 0.1791 -0.3805 -0.2115 -0.9293 -0.5473
Actin 0.0079 0.0006 0.0116 0.0013 0.0114 0.0026 0.0081 0.0002 0.0019 0.0446 0.001 0.0042 0.0027 0 0.0009 0.0007 0.0046 0.0003
Bud 0.0013 0.0024 0.0087 0.0017 0.0025 0.0026 0.0004 0.0003 0.0005 0.0006 0.0002 0.0006 0.0002 0.0004 0.0003 0.0002 0.0002 0.0001
Bud Neck 0.0032 0.0005 0.004 0.0006 0.0011 0.0017 0.0009 0.0008 0.0022 0.0005 0.0005 0.0013 0.0006 0.0005 0.0004 0.0007 0.0004 0.0004
Bud Periphery 0.0012 0.0018 0.0034 0.0048 0.0018 0.0014 0.0006 0.0005 0.0009 0.0008 0.0003 0.0019 0.0003 0.0005 0.0004 0.0003 0.0003 0.0002
Bud Site 0.0084 0.0048 0.0095 0.0006 0.0022 0.0008 0.0021 0.0017 0.0134 0.0019 0.0007 0.0013 0.0009 0.0015 0.0009 0.0005 0.0002 0.0002
Cell Periphery 0.0005 0.0004 0.0005 0.0001 0.0004 0.0003 0.0009 0.0008 0.001 0.0004 0.0004 0.0009 0.0004 0.0005 0.0004 0.0003 0.0001 0.0003
Cytoplasm 0.0444 0.0695 0.0567 0.0287 0.0275 0.0699 0.0451 0.0424 0.042 0.038 0.1051 0.0615 0.0533 0.0606 0.0685 0.033 0.0306 0.0529
Cytoplasmic Foci 0.0525 0.0436 0.0449 0.0257 0.0488 0.0465 0.0386 0.0127 0.0389 0.0345 0.0254 0.0296 0.0268 0.0257 0.021 0.0233 0.0188 0.0175
Eisosomes 0.0003 0 0.0001 0 0.0003 0 0.0004 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0 0.0003 0.0001 0
Endoplasmic Reticulum 0.006 0.004 0.004 0.0013 0.0036 0.003 0.0078 0.0026 0.0034 0.0029 0.0031 0.0048 0.0094 0.0054 0.0036 0.0021 0.0024 0.0017
Endosome 0.3397 0.2527 0.2716 0.3119 0.3685 0.2985 0.2978 0.2133 0.299 0.2259 0.2009 0.3019 0.3069 0.2311 0.2826 0.2522 0.3344 0.2308
Golgi 0.0105 0.0025 0.0072 0.0058 0.0209 0.0043 0.0093 0.0022 0.0104 0.0331 0.0098 0.0081 0.004 0.0021 0.0043 0.0019 0.0072 0.002
Lipid Particles 0.0329 0.0053 0.0155 0.0109 0.0304 0.0081 0.0209 0.0072 0.0176 0.0121 0.024 0.01 0.0137 0.0055 0.0125 0.0026 0.0054 0.0072
Mitochondria 0.0208 0.0042 0.0178 0.0064 0.0233 0.0073 0.0089 0.0029 0.0094 0.0201 0.0457 0.0089 0.0031 0.0021 0.003 0.0014 0.0029 0.0018
None 0.0563 0.0715 0.1007 0.0446 0.0472 0.0813 0.172 0.0895 0.1189 0.0845 0.262 0.0487 0.0975 0.0807 0.0684 0.0904 0.0664 0.0196
Nuclear Periphery 0.0183 0.0088 0.0127 0.0044 0.0062 0.0084 0.017 0.0065 0.0054 0.0047 0.0033 0.0079 0.023 0.0165 0.01 0.0055 0.0123 0.0049
Nucleolus 0.0062 0.005 0.006 0.0019 0.0041 0.0059 0.0034 0.0037 0.0043 0.0017 0.0011 0.0017 0.0018 0.0019 0.0074 0.0018 0.0007 0.0014
Nucleus 0.0154 0.0107 0.0155 0.0046 0.008 0.018 0.0066 0.0054 0.0071 0.0047 0.0033 0.0048 0.0143 0.0076 0.0103 0.0039 0.0026 0.0067
Peroxisomes 0.0211 0.0024 0.004 0.0129 0.0179 0.0016 0.0123 0.0004 0.0086 0.014 0.0038 0.0017 0.001 0.0006 0.0061 0.0005 0.0005 0.0083
Punctate Nuclear 0.0122 0.0098 0.0052 0.0018 0.0141 0.0056 0.0018 0.0004 0.0013 0.0013 0.0006 0.0009 0.0047 0.0011 0.0009 0.0019 0.001 0.0003
Vacuole 0.2924 0.4124 0.3231 0.4366 0.3176 0.3481 0.3003 0.4974 0.3377 0.4139 0.2691 0.4124 0.3481 0.4353 0.3875 0.4841 0.4356 0.4969
Vacuole Periphery 0.0485 0.0871 0.0775 0.0934 0.0423 0.0842 0.0447 0.1089 0.076 0.0598 0.0396 0.0869 0.0871 0.1205 0.1107 0.0922 0.0733 0.1465

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.0054 9.7564 12.3075 9.4621 10.6128 9.8172 12.5775 13.3161 11.1214 13.2875
Translational Efficiency 1.0683 0.801 0.5863 0.6181 0.6528 1.4747 0.3898 0.4273 0.6115 0.5737

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1296 1372 1540 1279 1312 935 2378 1464 2608 2307 3918 2743

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 759.64 823.93 972.57 904.39 703.40 798.18 882.49 943.35 731.35 813.49 917.90 925.18
Standard Deviation 118.70 131.33 128.45 141.47 131.74 110.63 114.00 139.37 128.54 124.01 127.70 141.69
Intensity Change Log 2 — 0.117206 0.356486 0.251629 — 0.182369 0.327235 0.423448 — 0.148902 0.342497 0.336793

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000469 0.000033 0.000107 0.000730 0.000030 0.000108 0.000045 0.000431 0.000248 0.000064 0.000069 0.000570
Bud Neck 0.005981 0.004171 0.007965 0.013122 0.008728 0.005636 0.009063 0.011591 0.007363 0.004765 0.008631 0.012305
Bud Site 0.021047 0.008809 0.005638 0.025028 0.006775 0.006181 0.010074 0.026648 0.013867 0.007744 0.008330 0.025893
Cell Periphery 0.000495 0.000078 0.000046 0.000129 0.000147 0.000171 0.000039 0.000098 0.000320 0.000116 0.000042 0.000112
Cytoplasm 0.058201 0.028813 0.046042 0.101839* 0.072879 0.019812 0.060091 0.081360 0.065585 0.025165 0.054569 0.090908
Cytoplasmic Foci 0.019358 0.033201 0.016338 0.017190 0.048289 0.057919 0.012543 0.026971 0.033912 0.043219 0.014034 0.022410
Eisosomes 0.000017 0.000009 0.000016 0.000016 0.000033 0.000025 0.000006 0.000014 0.000025 0.000016 0.000010 0.000015
Endoplasmic Reticulum 0.003662 0.002081 0.003800 0.002898 0.003311 0.003844 0.001285 0.001291 0.003486 0.002795 0.002274 0.002040
Endosome 0.066545 0.101744 0.056045 0.046406 0.096448 0.168960 0.050533 0.083265 0.081588 0.128986 0.052699 0.066079
Golgi 0.001361 0.004265 0.004642 0.008043 0.002719 0.010022 0.002431 0.007924 0.002044 0.006598 0.003300 0.007980
Lipid Particles 0.001806 0.001407 0.001125 0.001444 0.005047 0.005225 0.000494 0.001031 0.003436 0.002955 0.000742 0.001224
Mitochondria 0.003439 0.006087 0.002393 0.005512 0.003860 0.015450 0.002847 0.003883 0.003651 0.009882 0.002669 0.004642
Mitotic Spindle 0.000133 0.002547 0.021257 0.016200 0.000192 0.005846 0.003566 0.006745 0.000163 0.003884 0.010520 0.011154
None 0.000624 0.000480 0.000807 0.001531 0.005380 0.000671 0.000346 0.000350 0.003016 0.000557 0.000527 0.000901
Nuclear Periphery 0.000447 0.000695 0.010444 0.002487 0.001160 0.001127 0.001496 0.000902 0.000806 0.000870 0.005013 0.001641
Nuclear Periphery Foci 0.000134 0.000919 0.009822 0.001865 0.000632 0.001512 0.000771 0.000955 0.000385 0.001160 0.004329 0.001380
Nucleolus 0.000859 0.001370 0.022444 0.003846 0.005589 0.002431 0.003406 0.001602 0.003238 0.001800 0.010889 0.002649
Nucleus 0.008597 0.003942 0.045753 0.023994 0.028293 0.005354 0.017539 0.010052 0.018505 0.004515 0.028628 0.016553
Peroxisomes 0.000664 0.001447 0.001416 0.001399 0.001572 0.003182 0.000591 0.001997 0.001121 0.002150 0.000915 0.001718
Vacuole 0.655193* 0.540702* 0.463845* 0.656508* 0.553640* 0.414894* 0.675114* 0.637267* 0.604105* 0.489713* 0.592073* 0.646239*
Vacuole Periphery 0.150968* 0.257200* 0.280056* 0.069812 0.155276* 0.271626* 0.147722* 0.095622 0.153135* 0.263047* 0.199737* 0.083588

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.40 1.12 -0.33 -2.08 -1.77 -2.43 -1.62 -2.63 -2.03 -2.46 1.18 1.09 -1.17 -2.90 -2.81
Bud Neck 2.14 -3.63 -5.73 -7.28 -3.64 2.16 -1.29 -2.45 -5.04 -1.75 3.20 -3.17 -5.54 -8.99 -3.57
Bud Site 4.56 5.81 -0.59 -5.73 -7.17 0.48 -3.63 -8.65 -8.36 -6.84 4.20 3.75 -5.23 -9.86 -9.62
Cell Periphery 3.07 3.30 2.82 -1.68 -3.37 -0.63 8.02 3.07 2.10 -3.81 2.94 4.09 3.22 0.89 -5.08
Cytoplasm 5.73 0.85 -10.21 -16.30 -11.60 11.32 0.39 -4.98 -15.29 -5.70 11.55 0.42 -10.81 -21.92 -11.95
Cytoplasmic Foci -5.85 1.59 1.46 7.42 0.05 -2.35 15.12 8.11 8.41 -8.39 -4.13 13.31 7.14 10.44 -6.72
Eisosomes 4.17 -0.01 0.29 -4.82 0.40 1.58 6.37 4.49 3.03 -6.21 3.54 6.33 4.24 0.33 -4.81
Endoplasmic Reticulum 3.93 -0.49 2.24 -1.94 3.62 -0.85 6.87 7.20 4.46 0.92 2.03 4.47 5.99 3.19 2.72
Endosome -6.75 2.87 7.52 13.48 5.62 -9.06 11.61 4.49 12.16 -7.34 -10.37 10.77 7.61 16.41 -2.78
Golgi -6.05 -7.72 -3.84 -1.55 -1.35 -6.67 0.79 -3.06 1.91 -3.55 -8.43 -3.36 -4.85 -0.01 -3.77
Lipid Particles 0.85 1.72 0.96 -0.09 -1.33 -0.21 8.25 6.90 4.86 -2.45 0.92 8.07 6.33 4.21 -3.11
Mitochondria -2.47 1.42 -1.09 1.37 -2.64 -5.97 1.66 0.43 6.07 -0.96 -6.19 2.05 -0.52 5.70 -2.45
Mitotic Spindle -2.99 -10.15 -7.44 -5.85 2.29 -4.42 -6.29 -5.51 -0.65 -2.15 -5.24 -11.66 -9.23 -5.21 0.22
None 0.84 -1.25 -1.64 -2.05 -1.16 3.61 3.87 3.85 1.63 -0.23 3.71 3.74 3.23 -1.24 -1.37
Nuclear Periphery -1.84 -13.13 -5.75 -4.95 9.10 0.11 -2.45 0.50 0.41 2.86 -0.47 -12.94 -4.20 -3.90 8.77
Nuclear Periphery Foci -1.96 -10.66 -4.67 -1.56 8.32 -1.85 -1.88 -1.45 1.00 -0.12 -2.52 -10.75 -4.64 -0.51 7.31
Nucleolus -1.94 -13.95 -4.34 -3.21 11.59 3.53 2.25 4.67 1.34 4.71 3.06 -10.27 1.36 -1.95 11.87
Nucleus 3.71 -12.89 -10.01 -13.67 5.17 8.21 2.27 5.14 -6.16 5.02 9.12 -7.02 -1.91 -14.95 6.10
Peroxisomes -2.06 -3.15 -1.73 0.59 0.65 -2.92 4.31 -0.82 2.40 -4.99 -3.17 1.39 -1.86 1.75 -2.96
Vacuole 10.99 19.48 1.01 -9.66 -17.86 11.78 -12.84 -7.49 -19.18 4.60 14.46 2.43 -4.28 -19.09 -7.45
Vacuole Periphery -13.39 -14.32 16.74 29.03* 29.75* -12.52 2.78 11.80 21.91 11.40 -18.29 -7.31 19.77 35.52* 28.76*
Standard name
Human Ortholog
Description Palmitoyltransferase for Vac8p; required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions
Localization
Cell Percentages vacuole (28%), vacuolar membrane (18%), mixed (38%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pfa3

Pfa3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pfa3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available