Standard name
Human Ortholog
Description AAA-peroxin; heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; mutations in human PEX6 can lead to severe peroxisomal disorders and early death
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.11 0 0 0 0 0 0 – 0 0 0 0.12 0.27 0.13 – – – 0 0.06 0 0 0.06 0.11
Bud 0.07 0.14 0.07 0.11 0.11 0.12 0.12 0.16 – 0.16 0.12 0.13 0.08 0.13 0.05 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0.05 0 0 0.06 0 0.05 – 0.07 0.06 0.06 0.05 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0 0 0 0 – 0 0 0 0 0.06 0.05 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0.09 0 0.09 0.07 0.12 0.08 0.08 0.09 – 0 0 0.08 0 0.09 0.17 – – – 0.1 0.14 0.11 0.1 0.06 0.06
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.08 – – – 0 0 0 0 0 0
Mitochondria 0.55 0.88 0.42 0.35 0.32 0.5 0.46 0.37 – 0.57 0.59 0.54 0.29 0.15 0.05 – – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0.74 0.39 0.72 0.77 0.75 0.63 0.65 0.53 – 0.51 0.45 0.49 0.43 0.38 0.46 – – – 0.82 0.63 0.72 0.7 0.76 0.71
SpindlePole 0.16 0 0.22 0.21 0.31 0.22 0.26 0.33 – 0.23 0.26 0.26 0.43 0.38 0.31 – – – 0 0 0.06 0.06 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 7 17 8 2 1 1 0 10 – 4 7 10 16 59 28 – – – 4 8 9 5 26 41
Bud 20 20 29 31 44 58 96 124 – 101 56 74 11 29 10 – – – 1 0 2 3 2 3
Bud Neck 1 0 1 0 1 1 3 6 – 2 3 1 3 6 1 – – – 1 2 2 0 2 3
Bud Site 7 5 19 10 18 28 35 38 – 41 29 37 7 8 1 – – – – – – – – –
Cell Periphery 1 2 1 0 2 1 5 1 – 1 1 1 1 2 1 – – – 0 0 0 0 0 0
Cytoplasm 4 4 17 16 10 15 23 32 – 18 14 17 1 13 10 – – – 0 0 0 1 1 2
Endoplasmic Reticulum 0 3 1 0 0 0 0 1 – 1 0 1 0 4 5 – – – 0 0 2 1 3 6
Endosome 27 1 35 20 47 41 68 65 – 23 13 47 5 20 37 – – – 29 21 44 18 24 22
Golgi 2 0 0 0 1 0 0 0 – 0 0 0 1 7 18 – – – 2 6 6 2 6 8
Mitochondria 156 130 164 97 131 253 371 279 – 349 273 316 40 33 10 – – – 6 7 13 5 17 9
Nucleus 0 0 0 0 2 2 2 8 – 0 0 2 0 1 1 – – – 0 0 0 0 0 1
Nuclear Periphery 2 3 5 1 2 5 4 1 – 5 1 3 1 2 0 – – – 0 0 0 1 0 0
Nucleolus 5 3 6 2 11 11 16 19 – 9 10 13 0 1 4 – – – 0 1 0 0 0 0
Peroxisomes 211 58 282 213 304 316 522 403 – 313 210 286 59 84 101 – – – 246 98 285 132 313 281
SpindlePole 46 4 86 57 124 112 213 252 – 144 120 150 59 84 68 – – – 5 5 23 11 9 5
Vac/Vac Membrane 1 0 1 1 4 0 3 1 – 0 4 8 2 2 2 – – – 0 1 0 1 1 2
Unique Cell Count 285 148 389 278 406 503 808 764 615 464 587 137 220 220 300 156 397 190 413 394
Labelled Cell Count 490 250 655 450 702 844 1361 1240 1011 741 966 206 355 297 300 156 397 190 413 394


Peroxisomes

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 5.0 4.9 5.0 4.7 4.1 4.0 4.1 – 3.6 3.7 3.7 5.9 5.9 6.5 3.4 3.9 4.8 6.3 6.4 7.0
Std Deviation (1e-4) 0.9 1.0 1.3 1.5 1.5 1.6 1.3 1.5 – 1.0 1.0 1.0 0.8 1.0 0.9 0.7 0.7 0.9 1.5 1.5 2.0
Intensity Change (Log2) – – – 0.04 -0.05 -0.25 -0.3 -0.26 – -0.46 -0.42 -0.39 0.26 0.25 0.4 -0.52 -0.32 -0.03 0.36 0.38 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 4.6 9.4 5.4 – – –
Bud 1.6 0 0 0 0 – 0 0 0 0.2 2.3 -1.4 – – –
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Bud Site -0.8 0 0 0 0 – 0 0 0 0.1 -0.7 -2.9 – – –
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Cytoplasm 0.8 -1.5 -1.1 -1.4 -0.1 – -1.2 -1.1 -1.2 0 0.8 0.1 – – –
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Endosome -0.8 0 0 0 0 – 0 0 0 -2.0 0 2.9 – – –
Golgi 0 0 0 0 0 – 0 0 0 0 0 5.7 – – –
Mitochondria -1.9 0 0 0 0 – 0 0 0 -2.7 -6.9 -9.9 – – –
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Peroxisomes 1.2 0 0 0 0 – 0 0 0 -6.2 -8.3 -6.5 – – –
SpindlePole -0.5 0 0 0 0 – 0 0 0 4.7 4.2 2.4 – – –
Vacuole 0 0 0 0 0 – 0 0 0 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0554 0.0291 0.0498 0.0234 0.0262 0.0189 0.0307 0.0509 0.0451 0.0444 0.0302 0.0212 0.1112 0.0079 0.1152 0.0098 0.0009 0.0066
Bud 0.001 0.001 0.001 0.0061 0.0003 0.0001 0.0003 0.0002 0.0014 0.0001 0.0005 0.0001 0.0005 0 0.0003 0.0001 0.0001 0.0001
Bud Neck 0.0018 0.0007 0.0059 0.0006 0.0008 0.0009 0.0014 0.0065 0.0063 0.0082 0.0003 0.0008 0.0008 0 0.0004 0.0775 0.0006 0.0013
Bud Periphery 0.0013 0.001 0.0015 0.0031 0.0004 0.0002 0.0003 0.0001 0.0022 0.0001 0.0005 0.0001 0.0005 0 0.0004 0.0001 0.0001 0.0001
Bud Site 0.0069 0.0198 0.0067 0.0029 0.0009 0.0006 0.0058 0.019 0.01 0.0012 0.0078 0.0005 0.0045 0.0002 0.0008 0.0035 0.0003 0.0003
Cell Periphery 0.0002 0.0001 0.0001 0.0004 0.0001 0 0.0001 0 0.0003 0.0001 0.0001 0 0.0001 0 0 0.0001 0 0
Cytoplasm 0.0105 0.0027 0.0004 0.0037 0.0007 0.0007 0.0056 0.0001 0.0003 0.0002 0.0004 0.0002 0.0009 0 0.0003 0.0001 0.0032 0.0001
Cytoplasmic Foci 0.1164 0.1594 0.1004 0.0777 0.1251 0.0994 0.1077 0.1082 0.1051 0.0974 0.1128 0.108 0.1704 0.0337 0.164 0.0775 0.4258 0.188
Eisosomes 0.0002 0.0001 0.0001 0.0003 0.0001 0 0.0002 0 0.0001 0.0002 0.0001 0.0001 0.0007 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0031 0.0002 0 0.0013 0.0011 0.0002 0.0025 0 0 0.0001 0.0002 0.0002 0.0004 0 0 0 0.0001 0
Endosome 0.0416 0.0228 0.0139 0.0223 0.0532 0.0107 0.0162 0.002 0.0076 0.0062 0.0112 0.0103 0.0298 0 0.0151 0.0014 0.1182 0.0053
Golgi 0.0285 0.0275 0.0264 0.0229 0.0656 0.0178 0.0172 0.0098 0.0121 0.0233 0.0121 0.0192 0.0489 0.0011 0.054 0.0072 0.0179 0.0119
Lipid Particles 0.0629 0.029 0.0491 0.0562 0.078 0.0764 0.0407 0.0274 0.0252 0.0743 0.0414 0.0589 0.1499 0.0028 0.011 0.0225 0.0345 0.1209
Mitochondria 0.0145 0.0112 0.008 0.0401 0.0124 0.0069 0.003 0.006 0.0102 0.0039 0.0427 0.0091 0.0073 0.0024 0.0292 0.0025 0.0071 0.0019
None 0.0036 0.0045 0.0008 0.0026 0.0003 0.0006 0.0141 0.0001 0.0002 0.0001 0.0004 0.0002 0.0008 0 0.0007 0 0.0009 0
Nuclear Periphery 0.0073 0.0002 0 0.0025 0.0017 0.0017 0.0172 0 0 0.0001 0.0002 0.0001 0.0003 0 0 0 0.0001 0
Nucleolus 0.0015 0.0003 0.0003 0.0023 0.0001 0.0025 0.0031 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0003 0.0002
Nucleus 0.0046 0.0002 0.0001 0.0027 0.0002 0.0005 0.0051 0 0.0001 0 0.0001 0 0.0001 0 0 0 0.0001 0
Peroxisomes 0.6289 0.6783 0.7333 0.7181 0.6277 0.7528 0.7241 0.7689 0.7724 0.7385 0.7369 0.7692 0.4686 0.9517 0.6077 0.7965 0.3708 0.6612
Punctate Nuclear 0.0069 0.0115 0.0019 0.0023 0.0037 0.0087 0.0031 0.0006 0.0011 0.0012 0.0016 0.0014 0.0035 0.0001 0.0006 0.0007 0.0139 0.0021
Vacuole 0.0018 0.0004 0.0003 0.0049 0.0008 0.0002 0.0011 0 0.0001 0.0001 0.0004 0.0002 0.0002 0 0.0001 0 0.0038 0.0001
Vacuole Periphery 0.0009 0.0002 0.0001 0.0036 0.0006 0.0002 0.0008 0 0.0001 0.0001 0.0003 0.0001 0.0002 0 0.0001 0 0.0011 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.4158 7.9726 15.6184 24.2139 9.2506 11.0529 14.5907 15.1252 19.4257 21.1094
Translational Efficiency 0.3534 0.4515 0.2854 0.1593 0.2593 0.3362 0.3228 0.2439 0.1893 0.1032

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
968 879 493 1099 1271 1825 2020 1941 2239 2704 2513 3040

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 843.35 732.83 942.11 965.54 637.04 780.98 794.25 855.95 726.24 765.33 823.26 895.57
Standard Deviation 110.52 118.61 106.10 235.41 82.82 99.49 99.05 118.02 140.07 108.46 116.37 178.04
Intensity Change Log 2 — -0.202653 0.159764 0.195205 — 0.293902 0.318209 0.426143 — 0.032207 0.230089 0.299142

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.051815 0.019265 0.060658 0.327482* 0.020968 0.006974 0.046098 0.057977 0.034304 0.010970 0.048955 0.155406*
Bud Neck 0.002448 0.002900 0.005327 0.001900 0.003476 0.002792 0.001140 0.001616 0.003031 0.002827 0.001961 0.001719
Bud Site 0.014445 0.008214 0.028843 0.033195 0.007013 0.004474 0.008693 0.007977 0.010226 0.005690 0.012646 0.017094
Cell Periphery 0.000174 0.000089 0.000132 0.000362 0.000230 0.000056 0.000054 0.000074 0.000205 0.000067 0.000069 0.000178
Cytoplasm 0.003528 0.008180 0.007448 0.009411 0.003523 0.002352 0.003181 0.003197 0.003525 0.004247 0.004018 0.005443
Cytoplasmic Foci 0.094113 0.119990 0.115693 0.058604 0.103051 0.119169 0.055022 0.121274 0.099187 0.119436 0.066925 0.098618
Eisosomes 0.000112 0.000080 0.000094 0.000191 0.000315 0.000057 0.000080 0.000087 0.000227 0.000064 0.000083 0.000125
Endoplasmic Reticulum 0.000549 0.000064 0.001085 0.000496 0.000252 0.000043 0.000063 0.000099 0.000380 0.000050 0.000263 0.000242
Endosome 0.052010 0.048827 0.017353 0.023946 0.024602 0.058520 0.014056 0.081572 0.036451 0.055369 0.014703 0.060739
Golgi 0.027459 0.047271 0.013958 0.019574 0.042695 0.073294 0.010885 0.034081 0.036108 0.064835 0.011487 0.028837
Lipid Particles 0.012467 0.033045 0.021033 0.005393 0.046660 0.025519 0.014625 0.033934 0.031877 0.027965 0.015882 0.023616
Mitochondria 0.006966 0.005036 0.000955 0.004608 0.014491 0.015730 0.003212 0.006567 0.011238 0.012254 0.002769 0.005858
Mitotic Spindle 0.012465 0.007846 0.094479 0.018881 0.002087 0.007686 0.012585 0.016758 0.006574 0.007738 0.028651 0.017525
None 0.000018 0.000273 0.000124 0.000047 0.000690 0.000044 0.000023 0.000028 0.000400 0.000119 0.000043 0.000035
Nuclear Periphery 0.000086 0.000011 0.000080 0.000089 0.000071 0.000010 0.000009 0.000027 0.000077 0.000011 0.000023 0.000049
Nuclear Periphery Foci 0.000868 0.000293 0.002634 0.000909 0.000848 0.000120 0.000139 0.000403 0.000857 0.000176 0.000629 0.000586
Nucleolus 0.000048 0.000121 0.000589 0.000013 0.000495 0.000182 0.000012 0.000155 0.000302 0.000162 0.000125 0.000103
Nucleus 0.000223 0.000917 0.003288 0.000143 0.003744 0.000147 0.000027 0.000269 0.002222 0.000397 0.000667 0.000224
Peroxisomes 0.716821* 0.696447* 0.618856* 0.489275* 0.724258* 0.682116* 0.829826* 0.631389* 0.721043* 0.686775* 0.788438* 0.580013*
Vacuole 0.002471 0.001045 0.007296 0.004917 0.000457 0.000536 0.000255 0.002220 0.001328 0.000702 0.001636 0.003195
Vacuole Periphery 0.000913 0.000086 0.000075 0.000563 0.000076 0.000176 0.000015 0.000296 0.000438 0.000147 0.000027 0.000393

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 6.29 -0.91 -24.24 -28.69* -22.28* 7.23 -7.58 -9.61 -14.58 -2.65 9.64 -4.24 -22.31 -28.64* -19.14
Bud Neck -0.41 -2.31 0.70 0.90 2.84 0.58 2.34 1.81 1.68 -1.43 0.22 1.41 1.97 1.87 0.79
Bud Site 2.26 -4.29 -5.22 -7.64 -0.38 1.51 -0.96 -0.77 -2.84 0.26 3.04 -1.80 -4.13 -7.95 -2.52
Cell Periphery 1.71 0.37 -0.92 -1.36 -1.03 5.52 5.58 4.34 -0.99 -1.08 5.13 4.94 0.36 -1.52 -1.43
Cytoplasm -2.74 -2.62 -4.25 -0.80 -0.12 1.04 0.35 0.03 -1.38 -0.43 -1.22 -1.23 -3.04 -1.64 -1.68
Cytoplasmic Foci -3.81 -2.79 6.25 9.55 7.71 -2.79 8.84 -3.15 -0.51 -15.76 -4.70 7.88 -0.02 5.31 -9.20
Eisosomes 2.19 0.46 -4.86 -9.07 -6.82 1.81 1.64 1.59 -3.58 -0.76 2.01 1.75 1.25 -8.65 -5.19
Endoplasmic Reticulum 2.53 -3.22 -0.10 -4.01 3.56 1.90 1.71 1.34 -3.74 -2.49 3.18 0.26 0.96 -4.75 1.18
Endosome 0.62 8.02 6.33 6.38 -2.29 -10.88 5.21 -15.44 -5.44 -19.33 -6.70 9.93 -8.18 -1.78 -18.47
Golgi -5.80 5.15 2.85 8.55 -2.42 -8.63 13.98 3.34 12.66 -15.15 -11.07 14.95 3.84 15.31 -13.81
Lipid Particles -4.62 -2.46 3.16 6.91 4.86 4.68 7.87 2.82 -2.39 -6.69 1.22 5.76 2.89 1.69 -3.53
Mitochondria 0.93 3.59 1.15 0.27 -2.88 -0.50 5.81 3.78 4.81 -2.92 -0.59 6.24 3.63 4.57 -3.40
Mitotic Spindle 2.02 -9.03 -1.77 -3.91 8.31 -5.04 -6.96 -8.59 -4.79 -1.95 -0.56 -9.12 -6.23 -6.19 4.35
None -2.00 -2.12 -1.34 1.78 1.64 1.64 1.69 1.67 1.44 -0.80 1.21 1.55 1.62 1.95 0.98
Nuclear Periphery 2.36 -0.88 -0.16 -1.47 0.41 1.50 1.52 1.06 -3.44 -3.65 2.50 1.68 0.73 -1.98 -0.95
Nuclear Periphery Foci 1.24 -2.72 0.05 -1.05 2.69 1.90 1.67 1.06 -2.82 -1.72 2.29 -0.18 0.78 -1.95 1.18
Nucleolus -0.68 -1.39 1.48 2.91 1.48 1.08 1.70 1.15 0.20 -1.83 0.91 0.29 1.24 0.75 0.86
Nucleus -1.19 -3.52 0.26 1.28 3.56 2.49 2.57 2.37 -0.76 -1.50 2.18 1.41 2.38 0.64 2.55
Peroxisomes 1.42 6.86 15.83 14.33 6.04 4.02 -11.41 8.90 4.97 22.10* 4.03 -7.74 16.41 12.59 25.04*
Vacuole 1.61 -3.68 -2.92 -4.00 1.94 -0.44 1.23 -4.48 -4.42 -5.11 1.73 -2.17 -4.41 -5.79 -1.92
Vacuole Periphery 2.51 2.48 0.83 -1.88 -1.84 -2.71 3.09 -2.99 -1.56 -3.98 2.02 2.86 0.25 -2.36 -3.55
Standard name
Human Ortholog
Description AAA-peroxin; heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; mutations in human PEX6 can lead to severe peroxisomal disorders and early death
Localization
Cell Percentages peroxisome (68%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pex6

Pex6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pex6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available