Standard name
Human Ortholog
Description E2-like enzyme; involved in autophagy and cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p regulated by acetylation by NuA4 histone acetyltransferase Esa1p while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p; Atg12p-Atg5p conjugate enhances E2 activity of Atg3p by rearranging its catalytic site
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.08 0.09 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.97 0.99 0.95 0.87 0.79 0.76 0.77 0.71 0.73 0.73 1.0 0.99 0.99 0.99 0.97 0.97 0.93 0.94 0.93 0.9 0.91 0.82
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.08 0.13 0.26 0.37 0.36 0.4 0.45 0.46 0.48 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 1 0 0 1 0 1 0 0 1 0 0 0 0 1 1 1 0 0 1
Bud 0 1 1 2 3 7 4 8 12 10 9 13 1 1 0 0 1 0 1 1 0 1 0 5
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 2 0 1 1
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 10 6 3 1 1 9 3 5 9 3 9 11 6 5 14 20 14 17 0 0 0 0 0 0
Cytoplasm 439 322 265 304 405 389 454 437 316 303 238 307 282 400 474 260 177 175 438 305 262 225 254 282
Endoplasmic Reticulum 3 0 4 0 0 4 5 1 1 3 2 5 1 5 4 6 3 6 3 2 4 4 3 16
Endosome 0 0 0 0 2 0 0 0 1 0 0 0 1 0 0 1 0 0 4 3 1 2 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 0 2 0 1
Mitochondria 2 2 6 26 56 116 216 208 166 191 151 202 0 0 0 2 0 1 3 0 4 2 1 5
Nucleus 1 0 1 0 4 2 1 9 2 3 2 0 0 1 2 1 1 2 1 0 0 0 1 3
Nuclear Periphery 0 0 0 0 1 0 3 2 5 1 2 0 0 0 0 0 0 0 1 0 0 0 0 1
Nucleolus 0 0 0 0 1 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Vac/Vac Membrane 0 0 0 1 4 2 6 20 7 25 5 9 0 1 0 0 4 5 5 1 0 8 10 12
Unique Cell Count 443 322 272 308 428 446 577 575 411 424 325 419 283 406 477 263 182 181 472 323 281 251 280 343
Labelled Cell Count 456 331 280 334 477 530 692 693 522 540 419 547 291 414 494 290 200 206 472 323 281 251 280 343


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 6.6 4.7 4.7 4.9 4.7 4.5 4.9 4.8 4.4 4.8 5.1 7.4 7.5 8.2 8.9 9.1 9.5 8.1 8.2 8.6
Std Deviation (1e-4) 0.8 1.0 0.6 1.2 1.2 1.0 1.2 1.2 1.2 1.1 1.4 1.3 1.4 1.0 1.0 1.5 1.4 1.7 1.5 1.6 1.7
Intensity Change (Log2) – – – -0.01 0.05 0.01 -0.07 0.07 0.02 -0.11 0.03 0.11 0.66 0.67 0.8 0.93 0.96 1.02 0.79 0.8 0.87

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 2.5 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 1.4 0 0 1.6 3.7 3.6 4.2
Cytoplasm 1.1 -1.8 -4.7 -7.1 -7.7 -7.4 -8.6 -8.1 -8.2 2.2 1.0 2.2 1.2 -0.1 -0.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 10.6 0 0 0 12.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.1 0 4.1 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0066 0.0041 0.0038 0.0005 0.0203 0.0043 0.0149 0.0001 0.0209 0.1225 0.0006 0.0094 0.0002 0.0097 0.0002 0.0004 0 0.0005
Bud 0.0016 0.0089 0.0206 0.0003 0.0101 0.0038 0.0005 0.0001 0.0017 0.0144 0.0003 0.0003 0 0.0008 0.0001 0.0001 0 0
Bud Neck 0.0038 0.0015 0.0008 0.0006 0.0008 0.0014 0.0005 0.0001 0.0002 0.0023 0.0064 0.0006 0.0002 0.0002 0.0002 0.0001 0.0001 0.0002
Bud Periphery 0.002 0.0037 0.0056 0.0003 0.0102 0.0034 0.0004 0.0001 0.0021 0.0399 0.0002 0.0005 0.0001 0.0008 0.0001 0.0001 0 0.0001
Bud Site 0.0045 0.0427 0.0107 0.0005 0.0098 0.0009 0.0013 0.0006 0.0032 0.046 0.0077 0.0002 0.0003 0.0091 0.0002 0.0002 0 0.0001
Cell Periphery 0.0005 0.0007 0.0008 0.0001 0.0003 0.0002 0.0004 0.0002 0.0006 0.0053 0.0001 0.0002 0.0017 0.0005 0.0003 0.0002 0 0.0001
Cytoplasm 0.6641 0.7653 0.7533 0.7094 0.6533 0.8117 0.6793 0.8862 0.7544 0.6816 0.7223 0.7873 0.567 0.6695 0.7101 0.8732 0.9905 0.6663
Cytoplasmic Foci 0.0239 0.0097 0.0122 0.0214 0.0188 0.0135 0.012 0.0033 0.0072 0.0241 0.0304 0.0064 0.0032 0.0224 0.0092 0.007 0.0008 0.006
Eisosomes 0.0002 0.0001 0.0001 0 0.0001 0 0.0002 0 0.0001 0.001 0 0 0.0003 0.0003 0 0 0 0.0001
Endoplasmic Reticulum 0.0053 0.0034 0.0046 0.0011 0.0033 0.0042 0.0156 0.002 0.0037 0.0057 0.0055 0.004 0.0062 0.004 0.0018 0.0057 0.0004 0.0186
Endosome 0.0162 0.0053 0.0078 0.0293 0.0092 0.0044 0.0126 0.0017 0.006 0.01 0.0101 0.0026 0.0004 0.0109 0.0024 0.0054 0.0002 0.0258
Golgi 0.0031 0.0007 0.001 0.0129 0.0021 0.0005 0.0065 0.0002 0.0014 0.0054 0.0016 0.0004 0.0001 0.0146 0.0002 0.0004 0 0.0012
Lipid Particles 0.0043 0.0003 0.001 0.0065 0.0013 0.0001 0.0034 0.0001 0.0011 0.0019 0.0004 0.0003 0.0006 0.0009 0.0002 0.0002 0 0.0037
Mitochondria 0.0049 0.0004 0.0032 0.0202 0.0013 0.0009 0.002 0 0.011 0.0043 0.0004 0.0002 0.0001 0.022 0.0001 0.0001 0 0.0031
None 0.2437 0.1409 0.1558 0.1883 0.2454 0.1409 0.2239 0.1035 0.1801 0.0184 0.2 0.1845 0.4181 0.2257 0.2725 0.1017 0.0075 0.26
Nuclear Periphery 0.0014 0.001 0.0017 0.0004 0.0014 0.0008 0.0077 0.0002 0.0007 0.0014 0.0007 0.0004 0.0002 0.0005 0.0003 0.001 0.0001 0.007
Nucleolus 0.0008 0.0004 0.0006 0.0001 0.001 0.0003 0.0002 0 0.0002 0.0002 0.0007 0 0.0001 0.0001 0 0 0 0.0001
Nucleus 0.0022 0.0043 0.0064 0.0011 0.0022 0.0047 0.0087 0.0004 0.0012 0.0011 0.0015 0.0004 0.0002 0.0005 0.0004 0.0006 0.0002 0.0016
Peroxisomes 0.0032 0.001 0.0017 0.0043 0.0037 0.0002 0.0067 0 0.0004 0.0091 0.0049 0.0007 0 0.0056 0 0 0 0.0001
Punctate Nuclear 0.0036 0.0017 0.002 0.0008 0.0024 0.0021 0.0012 0.0001 0.0005 0.0025 0.0041 0.0003 0.0001 0.0002 0.0002 0.0003 0 0.0006
Vacuole 0.0036 0.0036 0.0055 0.0016 0.0026 0.0015 0.0015 0.001 0.0029 0.0024 0.0016 0.0012 0.001 0.0017 0.0016 0.0028 0.0003 0.0033
Vacuole Periphery 0.0006 0.0003 0.0006 0.0004 0.0004 0.0002 0.0007 0.0001 0.0005 0.0005 0.0002 0.0002 0 0.0003 0.0001 0.0003 0 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.2785 8.4577 3.9225 7.1719 5.3667 9.4559 10.9567 6.1324 9.9799 8.7396
Translational Efficiency 1.2022 1.2519 2.1005 1.798 1.87 1.5311 1.1999 2.5443 1.6639 1.6329

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1452 1293 1618 969 1393 567 413 2226 2845 1860 2031 3195

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 796.53 838.29 1054.76 1090.47 769.16 845.52 1055.78 1070.81 783.13 840.49 1054.97 1076.77
Standard Deviation 100.76 130.90 143.30 167.10 118.64 127.18 131.24 155.06 110.73 129.82 140.94 159.07
Intensity Change Log 2 — 0.073721 0.405114 0.453149 — 0.136555 0.456954 0.477347 — 0.104931 0.430814 0.465087

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000139 0.000269 0.000152 0.000479 0.000236 0.001526 0.000229 0.000393 0.000186 0.000652 0.000168 0.000419
Bud Neck 0.007588 0.010035 0.001247 0.002199 0.009892 0.032208 0.001163 0.001743 0.008716 0.016794 0.001230 0.001881
Bud Site 0.009363 0.033386 0.008493 0.030391 0.011339 0.044158 0.011602 0.015815 0.010330 0.036670 0.009125 0.020236
Cell Periphery 0.000170 0.000393 0.000077 0.000101 0.000184 0.000423 0.000095 0.000047 0.000177 0.000402 0.000080 0.000063
Cytoplasm 0.600319 0.610624 0.827759 0.709550 0.570330 0.405475 0.694071 0.849202 0.585636 0.548087 0.800574 0.806847
Cytoplasmic Foci 0.201115 0.186948 0.038565 0.062078 0.220866 0.276894 0.059109 0.034711 0.210785 0.214367 0.042743 0.043011
Eisosomes 0.000111 0.000048 0.000014 0.000019 0.000126 0.000189 0.000035 0.000012 0.000118 0.000091 0.000019 0.000014
Endoplasmic Reticulum 0.001435 0.000982 0.000537 0.000185 0.001866 0.000538 0.000511 0.000065 0.001646 0.000847 0.000532 0.000101
Endosome 0.019992 0.023019 0.003281 0.003521 0.028932 0.074119 0.004210 0.002290 0.024369 0.038596 0.003470 0.002663
Golgi 0.004231 0.005414 0.000187 0.000260 0.006445 0.015011 0.000132 0.002244 0.005315 0.008340 0.000176 0.001642
Lipid Particles 0.004012 0.001464 0.000611 0.000769 0.005944 0.004324 0.000828 0.000230 0.004958 0.002336 0.000655 0.000393
Mitochondria 0.001116 0.001932 0.000048 0.000157 0.001943 0.003632 0.000031 0.000443 0.001521 0.002450 0.000044 0.000356
Mitotic Spindle 0.002025 0.002273 0.003286 0.007039 0.000916 0.005349 0.000692 0.005710 0.001482 0.003211 0.002758 0.006113
None 0.002808 0.003851 0.002394 0.000604 0.004100 0.000158 0.002937 0.000829 0.003441 0.002725 0.002504 0.000760
Nuclear Periphery 0.000167 0.000071 0.000193 0.000061 0.000333 0.000063 0.000233 0.000030 0.000248 0.000068 0.000201 0.000040
Nuclear Periphery Foci 0.000889 0.000534 0.004269 0.002136 0.000968 0.000456 0.004851 0.000462 0.000928 0.000510 0.004387 0.000970
Nucleolus 0.000448 0.000334 0.000098 0.000094 0.000714 0.000211 0.000084 0.000087 0.000578 0.000296 0.000095 0.000090
Nucleus 0.031986 0.017098 0.034766 0.010313 0.028852 0.008131 0.038803 0.010312 0.030451 0.014365 0.035587 0.010312
Peroxisomes 0.001672 0.003254 0.000392 0.001174 0.002678 0.008963 0.002085 0.000957 0.002164 0.004994 0.000736 0.001022
Vacuole 0.108707 0.095330 0.073486 0.167181 0.101244 0.114974 0.178209 0.074259 0.105053 0.101318 0.094781 0.102441
Vacuole Periphery 0.001707 0.002741 0.000146 0.001691 0.002095 0.003196 0.000089 0.000160 0.001897 0.002880 0.000134 0.000624

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.13 -2.39 -4.27 -2.16 -3.76 -1.58 -0.32 -0.58 1.52 -0.37 -1.87 -0.18 -2.66 1.31 -2.75
Bud Neck -2.48 9.97 8.98 10.52 -1.93 -7.53 11.06 10.66 10.74 -1.04 -6.97 14.68 14.06 14.47 -1.71
Bud Site -9.91 1.41 -5.56 3.74 -6.00 -9.95 0.48 -0.74 9.51 -0.89 -13.44 2.26 -4.73 10.26 -5.71
Cell Periphery -2.50 9.75 7.44 3.42 -1.07 -1.02 6.50 10.11 1.65 9.00 -2.39 10.98 14.32 3.71 6.00
Cytoplasm -1.06 -21.24 -5.97 -4.68 11.30 12.57 -3.60 -31.37 -35.51 -15.52 4.58 -22.94 -30.39 -29.27 -4.17
Cytoplasmic Foci 2.16 34.90 28.53 22.82 -6.37 -5.94 30.00 37.35 29.12 4.75 -0.62 48.22 48.69 37.34 -0.45
Eisosomes 10.74 20.01 19.51 8.11 -1.47 -3.05 9.60 12.55 9.56 10.78 3.37 19.16 20.67 12.18 7.43
Endoplasmic Reticulum 1.28 4.74 7.20 2.69 6.73 3.64 3.67 5.08 6.47 9.83 2.74 5.44 7.94 3.52 9.66
Endosome -1.67 17.22 17.81 14.62 1.49 -7.97 15.96 16.98 13.33 2.99 -6.42 22.65 23.86 18.32 4.07
Golgi -1.59 7.30 7.29 12.07 -0.17 -4.10 10.24 6.79 6.74 -2.59 -3.71 12.42 8.44 9.81 -2.51
Lipid Particles 8.32 11.48 10.43 3.84 -1.72 1.05 7.95 8.90 2.87 7.88 4.71 12.19 13.00 4.26 5.45
Mitochondria -1.37 4.28 4.07 3.47 -0.84 -1.24 4.99 4.29 2.59 -2.13 -1.56 6.53 5.56 4.11 -2.22
Mitotic Spindle -0.16 -1.54 -1.56 -1.46 -0.18 -1.88 -1.54 -2.95 0.70 -1.90 -1.68 -2.25 -2.79 -0.57 -0.17
None -0.77 1.18 4.07 2.85 11.66 4.04 1.68 3.46 -7.87 4.57 0.78 2.23 4.97 2.52 11.34
Nuclear Periphery 3.05 -2.52 2.40 -1.97 7.44 3.34 -0.35 3.60 1.28 12.07 4.21 -0.72 4.47 1.18 12.22
Nuclear Periphery Foci 4.29 -9.68 -7.78 -10.26 5.95 3.51 -8.36 5.53 -0.43 9.78 5.79 -11.37 -1.12 -6.81 11.10
Nucleolus 2.16 7.59 7.76 8.66 0.96 4.00 5.14 5.07 3.71 -1.22 4.18 7.49 7.52 9.33 0.26
Nucleus 8.58 -6.04 13.90 4.60 22.64 10.65 -5.25 8.55 -6.19 11.71 11.69 -8.24 15.27 2.32 25.89
Peroxisomes -6.02 7.57 4.00 9.80 -3.32 -5.76 1.12 4.74 8.07 0.65 -6.97 5.29 6.09 11.78 -0.86
Vacuole 2.55 -0.70 -14.63 -16.17 -13.69 -2.18 -12.79 -0.94 1.64 12.63 0.78 -7.36 -9.59 -9.50 -0.42
Vacuole Periphery -3.26 7.40 1.31 2.72 -0.97 -3.36 14.12 13.81 10.52 -1.82 -4.35 13.73 6.55 9.36 -0.95
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description E2-like enzyme; involved in autophagy and cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p regulated by acetylation by NuA4 histone acetyltransferase Esa1p while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p; Atg12p-Atg5p conjugate enhances E2 activity of Atg3p by rearranging its catalytic site
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Atg3

Atg3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atg3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available