Standard name
Human Ortholog
Description Transcriptional co-repressor of MBF-regulated gene expression; Nrm1p associates stably with promoters via MCB binding factor (MBF) to repress transcription upon exit from G1 phase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.05 0.07 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.67 0.66 0.76 0.72 0.54 0.64 0.64 0.58 0.52 0.57 0.5 0.5 0.74 0.65 0.72 – 0.65 0.65 0.47 0.43 0.49 0.76 0.82 0.82
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.12 0 0 0.17 0.1 0.27 0.14 0.49 0.36 0.41 0.37 0 0 0 – 0.07 0.1 0 0.05 0.07 0 0 0
Nucleus 0.22 0.27 0.18 0.22 0.35 0.26 0.19 0.25 0.19 0.19 0.22 0.26 0.23 0.28 0.19 – 0.29 0.21 0.25 0.27 0.16 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.05 0.08 0.07 0.05 0 0
Vac/Vac Membrane 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.1 – 0 0.09 0.06 0 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 – 0 0 0 1 2 0 4 2
Bud 0 0 0 1 3 2 1 4 4 1 1 4 0 1 0 – 0 0 10 11 6 2 9 10
Bud Neck 0 0 3 2 4 7 3 14 6 6 0 7 0 3 0 – 0 0 1 1 0 0 1 2
Bud Site 0 0 0 0 0 0 2 1 2 1 1 2 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 1 0 1 1 1 0 0 0 1 0 – 2 1 0 0 1 1 2 1
Cytoplasm 123 90 134 98 59 127 106 165 195 154 132 145 192 322 308 – 89 68 97 65 95 112 292 307
Endoplasmic Reticulum 0 0 3 0 1 2 0 0 0 1 1 0 0 0 2 – 8 8 0 0 2 0 1 1
Endosome 1 3 2 1 0 1 0 1 0 0 1 0 1 4 6 – 0 1 2 0 2 3 4 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 1 0 0 0 1 1
Mitochondria 9 16 2 6 19 20 44 39 185 97 109 107 0 2 1 – 9 10 8 7 14 5 11 15
Nucleus 41 37 32 30 38 51 32 70 73 52 57 75 61 140 80 – 39 22 51 41 30 3 2 1
Nuclear Periphery 0 0 0 0 0 0 0 0 2 0 0 0 1 0 0 – 1 0 0 0 1 1 1 0
Nucleolus 2 2 0 1 1 1 0 4 3 2 4 2 0 0 0 – 3 4 7 2 3 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0
SpindlePole 1 1 1 0 0 2 2 8 8 7 8 10 1 1 0 – 1 2 9 11 14 7 7 8
Vac/Vac Membrane 13 2 4 1 3 3 0 8 1 0 2 6 7 32 45 – 3 9 12 5 9 3 2 4
Unique Cell Count 183 136 176 136 110 197 165 285 376 269 265 292 260 492 430 136 105 206 150 194 147 358 373
Labelled Cell Count 190 151 182 140 129 217 190 316 480 322 316 358 263 506 442 155 125 206 150 194 147 358 373


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 3.8 5.1 4.8 4.1 4.1 3.0 4.5 3.4 3.7 3.6 3.5 5.0 4.9 4.9 4.7 5.6 5.5 5.3 5.4 5.2
Std Deviation (1e-4) 0.9 0.8 1.0 1.4 1.2 1.2 0.8 1.4 1.1 1.1 1.1 0.7 1.1 0.9 1.2 1.7 1.6 1.4 1.8 1.7 1.4
Intensity Change (Log2) – – – -0.08 -0.3 -0.33 -0.79 -0.19 -0.59 -0.46 -0.51 -0.53 -0.03 -0.05 -0.05 -0.12 0.14 0.12 0.05 0.08 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.43 -0.1479 0.1361 0.5611 0.0484 -0.8313 3.0293 4.5499 4.9479 4.9027 4.4999 3.9822 1.5197 2.2563 2.7269 2.8745 2.183 1.4108
Actin 0.034 0.001 0.0105 0.012 0.0097 0.0107 0.0006 0.0001 0.0001 0 0.0001 0.0009 0.0841 0.0006 0.0019 0.0164 0.0001 0.0029
Bud 0.0112 0.0015 0.0071 0.0083 0.0069 0.0019 0.0081 0.0016 0.0002 0 0.0001 0.0031 0.0177 0.0113 0.0024 0.0002 0.0001 0.0028
Bud Neck 0.0054 0.001 0.0033 0.0023 0.0017 0.0048 0.0038 0.0013 0.0006 0.0002 0.0023 0.0055 0.0038 0.0016 0.0011 0.0018 0.0032 0.0066
Bud Periphery 0.0119 0.0019 0.005 0.0075 0.0096 0.0026 0.0108 0.001 0.0002 0 0.0002 0.0048 0.0209 0.0083 0.006 0.0003 0.0001 0.0047
Bud Site 0.021 0.0061 0.0028 0.0052 0.0013 0.0008 0.0128 0.0074 0.0007 0 0.0005 0.0005 0.028 0.0358 0.0137 0.0005 0.0004 0.0004
Cell Periphery 0.0017 0.0002 0.0002 0.0006 0.0003 0.0002 0.001 0.0002 0 0 0 0.0003 0.0014 0.001 0.0005 0.0001 0 0.0002
Cytoplasm 0.0781 0.0583 0.0154 0.0014 0.0099 0.1396 0.053 0.0582 0.0094 0.0001 0.0015 0.0789 0.0706 0.0494 0.0324 0.0017 0.0057 0.1278
Cytoplasmic Foci 0.0329 0.0055 0.0115 0.0019 0.0183 0.0176 0.006 0.0015 0.0005 0 0.0001 0.0057 0.0224 0.0038 0.0012 0.0095 0.0007 0.0124
Eisosomes 0.0005 0.0001 0.0001 0.0002 0.0001 0.0001 0.0004 0 0 0 0 0 0.0005 0.0001 0 0.0003 0 0.0001
Endoplasmic Reticulum 0.0087 0.0031 0.0024 0.0008 0.0027 0.0034 0.0022 0.0008 0.0002 0 0.0001 0.0013 0.0064 0.0015 0.0011 0.0013 0.0002 0.0017
Endosome 0.0319 0.0032 0.0063 0.0106 0.028 0.0092 0.0021 0.001 0.0004 0 0.0001 0.0011 0.012 0.0005 0.0015 0.014 0.0007 0.0031
Golgi 0.0129 0.0002 0.0036 0.008 0.0214 0.004 0.0001 0 0 0 0 0.0001 0.0056 0.0001 0.0004 0.007 0 0.0017
Lipid Particles 0.0217 0.001 0.008 0.0066 0.0086 0.008 0.0007 0.0001 0 0 0 0.0004 0.0043 0.0003 0.0005 0.0293 0.0002 0.002
Mitochondria 0.0259 0.0009 0.0098 0.0169 0.0309 0.0048 0.0021 0.0004 0.0003 0.0001 0.0002 0.0009 0.0049 0.001 0.004 0.007 0.0002 0.0014
None 0.3432 0.2455 0.0364 0.0008 0.0369 0.4498 0.4434 0.2501 0.0247 0 0.0032 0.5291 0.4358 0.4175 0.0268 0.0071 0.118 0.5156
Nuclear Periphery 0.0233 0.0214 0.0131 0.0104 0.0173 0.0117 0.0058 0.0027 0.0042 0.0003 0.0029 0.0039 0.0161 0.0063 0.0061 0.0049 0.0043 0.0039
Nucleolus 0.038 0.0219 0.0203 0.0223 0.0512 0.0215 0.0547 0.0205 0.0199 0.0107 0.0926 0.0182 0.0356 0.0122 0.0179 0.0117 0.035 0.0151
Nucleus 0.2121 0.58 0.8111 0.8733 0.7253 0.2709 0.3479 0.6287 0.9195 0.9879 0.8397 0.3019 0.1746 0.4146 0.8477 0.8502 0.7849 0.2646
Peroxisomes 0.0169 0.0003 0.0083 0.0015 0.0051 0.0091 0.0003 0 0 0 0 0.0002 0.0078 0.0002 0.0003 0.0295 0 0.0017
Punctate Nuclear 0.0356 0.0423 0.022 0.0043 0.0059 0.0257 0.0317 0.0197 0.0182 0.0006 0.0558 0.0396 0.0288 0.031 0.0281 0.0063 0.0457 0.0285
Vacuole 0.0281 0.0038 0.0017 0.0037 0.0051 0.0027 0.0111 0.0044 0.0006 0 0.0003 0.0029 0.0155 0.0026 0.005 0.0004 0.0004 0.0024
Vacuole Periphery 0.0053 0.0008 0.001 0.0015 0.0037 0.0009 0.0014 0.0002 0.0002 0 0.0001 0.0005 0.0034 0.0002 0.0013 0.0005 0.0001 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.0105 26.3034 7.8073 7.534 21.9361 10.2927 30.2522 13.4625 8.4328 29.0184
Translational Efficiency 2.196 2.8427 1.5292 1.3553 2.4464 4.8609 1.5524 1.81 1.7338 1.5915

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1210 730 2714 1689 1942 2294 1623 1510 3152 3024 4337 3199

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 550.92 755.12 819.50 793.62 653.95 795.70 838.41 820.47 614.40 785.90 826.58 806.29
Standard Deviation 65.88 133.54 101.94 124.38 78.43 98.22 102.22 117.86 89.26 109.20 102.45 122.09
Intensity Change Log 2 — 0.454863 0.572901 0.526606 — 0.283044 0.358476 0.327270 — 0.364154 0.460488 0.421844

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000148 0.001241 0.000251 0.001878 0.000100 0.000192 0.000545 0.001018 0.000118 0.000445 0.000361 0.001472
Bud Neck 0.013480 0.031387 0.017145 0.012888 0.005775 0.032223 0.010894 0.015132 0.008733 0.032021 0.014805 0.013947
Bud Site 0.004849 0.014020 0.004710 0.027266 0.002360 0.009059 0.004003 0.021528 0.003315 0.010257 0.004445 0.024558
Cell Periphery 0.000653 0.000395 0.000317 0.000370 0.000265 0.000377 0.000160 0.000321 0.000414 0.000382 0.000259 0.000347
Cytoplasm 0.260333* 0.245291* 0.179529* 0.257266* 0.368518* 0.328025* 0.123034* 0.152242* 0.326988* 0.308053* 0.158387* 0.207692*
Cytoplasmic Foci 0.142170 0.106267 0.003187 0.013567 0.061917 0.092138 0.002975 0.002792 0.092725 0.095549 0.003107 0.008481
Eisosomes 0.000191 0.000122 0.000055 0.000050 0.000052 0.000105 0.000030 0.000037 0.000106 0.000109 0.000046 0.000044
Endoplasmic Reticulum 0.001821 0.006381 0.001727 0.002803 0.000853 0.000990 0.004195 0.005312 0.001224 0.002292 0.002651 0.003987
Endosome 0.004801 0.012901 0.000897 0.013264 0.002148 0.007012 0.001189 0.001246 0.003167 0.008434 0.001006 0.007591
Golgi 0.002582 0.002282 0.000094 0.005986 0.000334 0.002068 0.000105 0.000171 0.001197 0.002120 0.000098 0.003241
Lipid Particles 0.020740 0.009071 0.000516 0.003133 0.003500 0.006544 0.000716 0.000800 0.010118 0.007154 0.000590 0.002032
Mitochondria 0.019123 0.011209 0.001497 0.006264 0.001234 0.010087 0.001302 0.002017 0.008101 0.010358 0.001424 0.004259
Mitotic Spindle 0.000893 0.016284 0.009037 0.051727 0.000977 0.005847 0.008892 0.010450 0.000944 0.008367 0.008983 0.032243
None 0.071757 0.024006 0.014923 0.018432 0.032461 0.021249 0.008879 0.016199 0.047546 0.021915 0.012661 0.017378
Nuclear Periphery 0.001120 0.002279 0.001653 0.003043 0.000661 0.000908 0.001667 0.001766 0.000837 0.001239 0.001658 0.002440
Nuclear Periphery Foci 0.000935 0.002238 0.000202 0.001138 0.000793 0.000512 0.000623 0.001430 0.000848 0.000928 0.000360 0.001276
Nucleolus 0.016426 0.016674 0.008080 0.003993 0.012415 0.018768 0.006777 0.008247 0.013954 0.018262 0.007592 0.006001
Nucleus 0.420065* 0.445997* 0.749505* 0.516168* 0.496081* 0.438270* 0.813419* 0.740683* 0.466900* 0.440136* 0.773423* 0.622144*
Peroxisomes 0.007512 0.014527 0.000193 0.002383 0.000909 0.006438 0.000233 0.000477 0.003444 0.008391 0.000208 0.001483
Vacuole 0.008980 0.034835 0.006279 0.051183 0.008316 0.017823 0.010156 0.017234 0.008571 0.021930 0.007730 0.035158
Vacuole Periphery 0.001420 0.002593 0.000203 0.007198 0.000332 0.001362 0.000205 0.000897 0.000750 0.001659 0.000204 0.004224

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.29 -9.09 -2.49 0.42 -1.80 -4.21 -16.84 -8.30 -7.52 -3.04 -7.13 -18.41 -4.79 -3.12 -2.78
Bud Neck -5.81 -6.13 -0.45 5.97 7.36 -15.13 -9.26 -10.86 5.83 -2.46 -14.82 -12.68 -8.44 9.29 4.03
Bud Site -4.99 -0.54 -6.32 -1.34 -6.25 -7.12 -3.77 -6.73 -3.50 -5.47 -8.19 -3.41 -9.53 -4.40 -8.26
Cell Periphery 3.54 5.72 5.65 3.47 0.48 -4.34 5.71 0.21 3.94 -4.14 1.00 6.50 4.95 5.30 -1.42
Cytoplasm 2.01 13.23 4.95 2.01 -8.58 4.24 31.07 25.90 23.28 -4.04 2.79 31.58 22.22 19.13 -8.22
Cytoplasmic Foci 4.90 24.74 23.32 14.14 -7.53 -6.87 21.42 21.61 26.45 -0.08 -0.21 31.77 30.21 29.09 -6.74
Eisosomes 4.45 9.68 10.65 7.82 -0.98 -6.02 4.68 2.18 6.83 -2.58 -0.45 8.52 10.20 8.51 -1.24
Endoplasmic Reticulum -8.24 -3.82 -6.92 4.32 -5.11 -1.44 -18.87 -18.19 -17.75 -2.21 -6.55 -17.29 -18.61 -11.85 -6.23
Endosome -5.02 5.95 -2.09 3.21 -6.67 -9.94 3.86 4.08 12.17 -0.11 -9.35 7.49 -2.21 5.75 -6.68
Golgi 0.86 7.45 -0.31 -0.89 -4.30 -7.08 6.13 4.47 7.78 -3.23 -3.64 8.25 -1.03 1.34 -4.36
Lipid Particles 6.19 12.08 10.65 6.04 -2.91 -4.78 9.95 9.34 9.79 -1.52 3.60 14.02 11.74 9.85 -3.23
Mitochondria 3.25 8.03 6.74 4.06 -4.06 -9.54 -2.12 -5.15 8.46 -3.79 -1.91 7.60 5.49 8.34 -4.80
Mitotic Spindle -5.35 -9.28 -10.97 -3.79 -6.53 -4.55 -5.68 -6.68 -4.25 -1.63 -7.16 -10.52 -12.71 -7.84 -6.20
None 9.03 12.58 12.35 3.09 0.22 5.53 13.85 9.21 4.99 -3.90 11.04 17.04 15.32 6.28 -1.79
Nuclear Periphery -3.42 -6.83 -5.92 -2.34 -3.71 -2.45 -14.70 -13.33 -11.05 -2.07 -3.55 -16.02 -9.65 -7.41 -4.18
Nuclear Periphery Foci -3.60 3.34 -0.32 3.40 -3.61 1.73 -0.17 -1.93 -4.07 -1.90 0.38 1.54 -1.64 -2.52 -3.71
Nucleolus 0.12 2.46 8.88 6.84 7.99 -3.11 3.38 2.90 6.45 -0.57 -2.56 3.34 8.21 10.93 5.38
Nucleus -2.34 -30.99 -12.88 -8.47 17.97 5.32 -32.03 -22.21 -28.60 7.75 2.59 -43.23 -22.45 -24.91 17.66
Peroxisomes -3.38 11.32 9.23 7.14 -4.52 -10.67 7.38 2.95 11.32 -2.02 -7.59 12.36 8.86 12.43 -4.97
Vacuole -8.34 -0.16 -17.34 -7.45 -17.46 -6.95 -5.96 -9.35 -5.52 -4.35 -10.63 -4.65 -19.50 -12.52 -17.20
Vacuole Periphery -1.79 4.51 -2.76 -1.19 -4.66 -6.89 5.55 -1.18 2.45 -1.65 -4.48 5.17 -3.44 -1.20 -4.93
Standard name
Human Ortholog
Description Transcriptional co-repressor of MBF-regulated gene expression; Nrm1p associates stably with promoters via MCB binding factor (MBF) to repress transcription upon exit from G1 phase
Localization
Cell Percentages nucleus (49%), cytoplasm (12%), mixed (28%)
Cell Cycle Regulation Yes
cytoplasm - T/G1 (1.5e-07)
nucleus - S/G2 (1.2e-05)
nucleus - MA (2.4e-05)
Subcompartmental Group N/A

Nrm1

Nrm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nrm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available