Standard name | |
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Human Ortholog | |
Description | Transcriptional co-repressor of MBF-regulated gene expression; Nrm1p associates stably with promoters via MCB binding factor (MBF) to repress transcription upon exit from G1 phase |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 2 | 0 | 4 | 2 |
Bud | 0 | 0 | 0 | 1 | 3 | 2 | 1 | 4 | 4 | 1 | 1 | 4 | 0 | 1 | 0 | – | 0 | 0 | 10 | 11 | 6 | 2 | 9 | 10 |
Bud Neck | 0 | 0 | 3 | 2 | 4 | 7 | 3 | 14 | 6 | 6 | 0 | 7 | 0 | 3 | 0 | – | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 1 | 1 | 2 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | – | 2 | 1 | 0 | 0 | 1 | 1 | 2 | 1 |
Cytoplasm | 123 | 90 | 134 | 98 | 59 | 127 | 106 | 165 | 195 | 154 | 132 | 145 | 192 | 322 | 308 | – | 89 | 68 | 97 | 65 | 95 | 112 | 292 | 307 |
Endoplasmic Reticulum | 0 | 0 | 3 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | – | 8 | 8 | 0 | 0 | 2 | 0 | 1 | 1 |
Endosome | 1 | 3 | 2 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 4 | 6 | – | 0 | 1 | 2 | 0 | 2 | 3 | 4 | 4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 |
Mitochondria | 9 | 16 | 2 | 6 | 19 | 20 | 44 | 39 | 185 | 97 | 109 | 107 | 0 | 2 | 1 | – | 9 | 10 | 8 | 7 | 14 | 5 | 11 | 15 |
Nucleus | 41 | 37 | 32 | 30 | 38 | 51 | 32 | 70 | 73 | 52 | 57 | 75 | 61 | 140 | 80 | – | 39 | 22 | 51 | 41 | 30 | 3 | 2 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | – | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 |
Nucleolus | 2 | 2 | 0 | 1 | 1 | 1 | 0 | 4 | 3 | 2 | 4 | 2 | 0 | 0 | 0 | – | 3 | 4 | 7 | 2 | 3 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 1 | 1 | 1 | 0 | 0 | 2 | 2 | 8 | 8 | 7 | 8 | 10 | 1 | 1 | 0 | – | 1 | 2 | 9 | 11 | 14 | 7 | 7 | 8 |
Vac/Vac Membrane | 13 | 2 | 4 | 1 | 3 | 3 | 0 | 8 | 1 | 0 | 2 | 6 | 7 | 32 | 45 | – | 3 | 9 | 12 | 5 | 9 | 3 | 2 | 4 |
Unique Cell Count | 183 | 136 | 176 | 136 | 110 | 197 | 165 | 285 | 376 | 269 | 265 | 292 | 260 | 492 | 430 | 136 | 105 | 206 | 150 | 194 | 147 | 358 | 373 | |
Labelled Cell Count | 190 | 151 | 182 | 140 | 129 | 217 | 190 | 316 | 480 | 322 | 316 | 358 | 263 | 506 | 442 | 155 | 125 | 206 | 150 | 194 | 147 | 358 | 373 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.2 | 3.8 | 5.1 | 4.8 | 4.1 | 4.1 | 3.0 | 4.5 | 3.4 | 3.7 | 3.6 | 3.5 | 5.0 | 4.9 | 4.9 | 4.7 | 5.6 | 5.5 | 5.3 | 5.4 | 5.2 |
Std Deviation (1e-4) | 0.9 | 0.8 | 1.0 | 1.4 | 1.2 | 1.2 | 0.8 | 1.4 | 1.1 | 1.1 | 1.1 | 0.7 | 1.1 | 0.9 | 1.2 | 1.7 | 1.6 | 1.4 | 1.8 | 1.7 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.3 | -0.33 | -0.79 | -0.19 | -0.59 | -0.46 | -0.51 | -0.53 | -0.03 | -0.05 | -0.05 | -0.12 | 0.14 | 0.12 | 0.05 | 0.08 | 0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.43 | -0.1479 | 0.1361 | 0.5611 | 0.0484 | -0.8313 | 3.0293 | 4.5499 | 4.9479 | 4.9027 | 4.4999 | 3.9822 | 1.5197 | 2.2563 | 2.7269 | 2.8745 | 2.183 | 1.4108 |
Actin | 0.034 | 0.001 | 0.0105 | 0.012 | 0.0097 | 0.0107 | 0.0006 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0009 | 0.0841 | 0.0006 | 0.0019 | 0.0164 | 0.0001 | 0.0029 |
Bud | 0.0112 | 0.0015 | 0.0071 | 0.0083 | 0.0069 | 0.0019 | 0.0081 | 0.0016 | 0.0002 | 0 | 0.0001 | 0.0031 | 0.0177 | 0.0113 | 0.0024 | 0.0002 | 0.0001 | 0.0028 |
Bud Neck | 0.0054 | 0.001 | 0.0033 | 0.0023 | 0.0017 | 0.0048 | 0.0038 | 0.0013 | 0.0006 | 0.0002 | 0.0023 | 0.0055 | 0.0038 | 0.0016 | 0.0011 | 0.0018 | 0.0032 | 0.0066 |
Bud Periphery | 0.0119 | 0.0019 | 0.005 | 0.0075 | 0.0096 | 0.0026 | 0.0108 | 0.001 | 0.0002 | 0 | 0.0002 | 0.0048 | 0.0209 | 0.0083 | 0.006 | 0.0003 | 0.0001 | 0.0047 |
Bud Site | 0.021 | 0.0061 | 0.0028 | 0.0052 | 0.0013 | 0.0008 | 0.0128 | 0.0074 | 0.0007 | 0 | 0.0005 | 0.0005 | 0.028 | 0.0358 | 0.0137 | 0.0005 | 0.0004 | 0.0004 |
Cell Periphery | 0.0017 | 0.0002 | 0.0002 | 0.0006 | 0.0003 | 0.0002 | 0.001 | 0.0002 | 0 | 0 | 0 | 0.0003 | 0.0014 | 0.001 | 0.0005 | 0.0001 | 0 | 0.0002 |
Cytoplasm | 0.0781 | 0.0583 | 0.0154 | 0.0014 | 0.0099 | 0.1396 | 0.053 | 0.0582 | 0.0094 | 0.0001 | 0.0015 | 0.0789 | 0.0706 | 0.0494 | 0.0324 | 0.0017 | 0.0057 | 0.1278 |
Cytoplasmic Foci | 0.0329 | 0.0055 | 0.0115 | 0.0019 | 0.0183 | 0.0176 | 0.006 | 0.0015 | 0.0005 | 0 | 0.0001 | 0.0057 | 0.0224 | 0.0038 | 0.0012 | 0.0095 | 0.0007 | 0.0124 |
Eisosomes | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0005 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0087 | 0.0031 | 0.0024 | 0.0008 | 0.0027 | 0.0034 | 0.0022 | 0.0008 | 0.0002 | 0 | 0.0001 | 0.0013 | 0.0064 | 0.0015 | 0.0011 | 0.0013 | 0.0002 | 0.0017 |
Endosome | 0.0319 | 0.0032 | 0.0063 | 0.0106 | 0.028 | 0.0092 | 0.0021 | 0.001 | 0.0004 | 0 | 0.0001 | 0.0011 | 0.012 | 0.0005 | 0.0015 | 0.014 | 0.0007 | 0.0031 |
Golgi | 0.0129 | 0.0002 | 0.0036 | 0.008 | 0.0214 | 0.004 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0056 | 0.0001 | 0.0004 | 0.007 | 0 | 0.0017 |
Lipid Particles | 0.0217 | 0.001 | 0.008 | 0.0066 | 0.0086 | 0.008 | 0.0007 | 0.0001 | 0 | 0 | 0 | 0.0004 | 0.0043 | 0.0003 | 0.0005 | 0.0293 | 0.0002 | 0.002 |
Mitochondria | 0.0259 | 0.0009 | 0.0098 | 0.0169 | 0.0309 | 0.0048 | 0.0021 | 0.0004 | 0.0003 | 0.0001 | 0.0002 | 0.0009 | 0.0049 | 0.001 | 0.004 | 0.007 | 0.0002 | 0.0014 |
None | 0.3432 | 0.2455 | 0.0364 | 0.0008 | 0.0369 | 0.4498 | 0.4434 | 0.2501 | 0.0247 | 0 | 0.0032 | 0.5291 | 0.4358 | 0.4175 | 0.0268 | 0.0071 | 0.118 | 0.5156 |
Nuclear Periphery | 0.0233 | 0.0214 | 0.0131 | 0.0104 | 0.0173 | 0.0117 | 0.0058 | 0.0027 | 0.0042 | 0.0003 | 0.0029 | 0.0039 | 0.0161 | 0.0063 | 0.0061 | 0.0049 | 0.0043 | 0.0039 |
Nucleolus | 0.038 | 0.0219 | 0.0203 | 0.0223 | 0.0512 | 0.0215 | 0.0547 | 0.0205 | 0.0199 | 0.0107 | 0.0926 | 0.0182 | 0.0356 | 0.0122 | 0.0179 | 0.0117 | 0.035 | 0.0151 |
Nucleus | 0.2121 | 0.58 | 0.8111 | 0.8733 | 0.7253 | 0.2709 | 0.3479 | 0.6287 | 0.9195 | 0.9879 | 0.8397 | 0.3019 | 0.1746 | 0.4146 | 0.8477 | 0.8502 | 0.7849 | 0.2646 |
Peroxisomes | 0.0169 | 0.0003 | 0.0083 | 0.0015 | 0.0051 | 0.0091 | 0.0003 | 0 | 0 | 0 | 0 | 0.0002 | 0.0078 | 0.0002 | 0.0003 | 0.0295 | 0 | 0.0017 |
Punctate Nuclear | 0.0356 | 0.0423 | 0.022 | 0.0043 | 0.0059 | 0.0257 | 0.0317 | 0.0197 | 0.0182 | 0.0006 | 0.0558 | 0.0396 | 0.0288 | 0.031 | 0.0281 | 0.0063 | 0.0457 | 0.0285 |
Vacuole | 0.0281 | 0.0038 | 0.0017 | 0.0037 | 0.0051 | 0.0027 | 0.0111 | 0.0044 | 0.0006 | 0 | 0.0003 | 0.0029 | 0.0155 | 0.0026 | 0.005 | 0.0004 | 0.0004 | 0.0024 |
Vacuole Periphery | 0.0053 | 0.0008 | 0.001 | 0.0015 | 0.0037 | 0.0009 | 0.0014 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0005 | 0.0034 | 0.0002 | 0.0013 | 0.0005 | 0.0001 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 25.0105 | 26.3034 | 7.8073 | 7.534 | 21.9361 | 10.2927 | 30.2522 | 13.4625 | 8.4328 | 29.0184 |
Translational Efficiency | 2.196 | 2.8427 | 1.5292 | 1.3553 | 2.4464 | 4.8609 | 1.5524 | 1.81 | 1.7338 | 1.5915 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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