Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; component of the Middle domain of mediator; required for regulation of RNA polymerase II activity; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0 – 0 0 0 0 0 0.06 0.1 0.14 0.09 0.08 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0.09 0.56 0.34 0.15 0.37 – 0.42 0.51 0.35 0.46 0.43 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Nucleus 0.93 0.88 0.9 0.83 0.87 0.77 – 0.69 0.64 0.64 0.68 0.61 0.85 0.79 0.74 0.78 0.77 0.76 0.88 0.85 0.92 0.68 0.69 0.68
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.17 0.25 0.06 0.18 0.12 0.23 – 0.25 0.34 0.41 0.34 0.4 0.2 0.2 0.2 0.12 0.13 0.16 0.07 0.06 0 0.19 0.15 0.13
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 – 6 1 2 2 2 0 0 0 0 0 0 1 0 0 2 3 6
Bud Neck 0 0 0 0 0 0 – 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 1 0 0 1 0 6 – 11 9 11 15 16 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 2 1 3 5 15 6 – 11 9 8 3 13 13 23 47 31 28 21 0 0 0 0 3 2
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 4 5 1 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 1 2 5 9 3 5 2 1 0 0 1 3 10
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 2 3 0 0 0 0 0 5 4
Mitochondria 36 5 46 68 41 110 – 245 162 149 149 245 7 6 1 17 17 9 2 1 0 0 2 4
Nucleus 309 49 74 166 236 229 – 402 202 270 220 354 191 178 259 260 272 153 300 62 81 66 189 228
Nuclear Periphery 2 1 0 2 2 7 – 18 7 9 9 6 1 1 1 5 5 3 0 0 0 0 0 0
Nucleolus 58 14 5 37 33 68 – 146 108 174 110 233 45 45 71 41 47 33 25 4 3 18 41 43
Peroxisomes 0 0 0 0 1 0 – 0 0 0 0 0 0 1 5 0 0 1 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 2 – 2 0 0 1 3 1 1 7 2 5 0 1 0 0 2 3 7
Vac/Vac Membrane 2 0 0 3 1 2 – 3 2 2 3 7 3 3 3 4 14 6 6 1 1 3 17 24
Unique Cell Count 332 56 82 200 270 297 586 317 421 325 576 226 225 348 334 353 202 341 73 89 98 277 337
Labelled Cell Count 411 70 128 282 329 431 845 500 626 512 881 263 264 404 370 401 229 341 73 89 98 277 337


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.7 6.7 4.3 4.9 5.5 4.7 – 4.7 4.2 4.8 4.6 4.7 7.3 7.6 7.1 10.2 10.1 9.2 6.3 7.5 7.9
Std Deviation (1e-4) 1.3 1.1 1.1 1.2 1.5 1.1 – 1.3 1.1 1.4 1.3 1.4 2.0 2.6 2.3 2.6 2.5 2.2 1.6 2.1 2.1
Intensity Change (Log2) – – – 0.2 0.38 0.12 – 0.14 -0.01 0.16 0.09 0.12 0.77 0.83 0.73 1.25 1.24 1.1 0.56 0.8 0.88


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 – 0 0 0 0 0 0 1.8 2.5 1.7 1.4 1.8
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -3.4 -7.5 0 – 0 0 0 0 0 -10.9 -11.0 -14.6 -11.5 -11.9 -10.0
Nucleus -1.6 -0.7 -2.6 – -4.1 -4.6 -4.7 -4.1 -5.1 -1.3 -2.3 -3.1 -2.5 -2.7 -2.8
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.7 1.6 0 – 0 0 0 0 0 2.9 2.9 3.1 1.6 1.8 2.3
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6769 2.1845 2.0149 1.3312 1.9612 1.8685 1.4749 1.9106 1.6096 1.0432 1.933 1.2667 3.2508 3.9104 3.3841 3.3269 3.3459 3.2902
Actin 0.0045 0 0.0027 0.0025 0.0016 0.0007 0.047 0 0.004 0.0025 0.0006 0.0008 0.0096 0 0.0015 0.0021 0.0009 0.0001
Bud 0.0001 0 0.0003 0.0001 0.0006 0 0.0005 0 0.0014 0.0001 0.0123 0.0001 0 0 0 0.0001 0.0001 0.0001
Bud Neck 0.0003 0.0001 0.001 0.0003 0.0016 0.001 0.0006 0 0.0006 0.0004 0.0009 0.0009 0.0002 0.0001 0.0004 0.0003 0.0012 0.0017
Bud Periphery 0.0003 0.0001 0.0004 0.0001 0.0026 0.0001 0.0008 0 0.0019 0.0001 0.0272 0.0002 0.0001 0 0.0001 0.0002 0.0003 0.0002
Bud Site 0.0004 0.0001 0.0029 0.0002 0.0016 0.0001 0.0015 0 0.0052 0.0003 0.0063 0.0001 0.0001 0.0001 0.0003 0.0006 0.0004 0.0002
Cell Periphery 0.0001 0 0.0001 0 0.0006 0 0.0002 0 0.0001 0 0.0008 0 0 0 0.0002 0.0001 0.0001 0.0001
Cytoplasm 0.0008 0 0.0007 0.0046 0.0004 0.0003 0.0126 0 0.0025 0.001 0.0019 0 0.0002 0 0.0004 0.0007 0.0012 0.0001
Cytoplasmic Foci 0.0061 0 0.005 0.0071 0.0052 0.0003 0.0095 0 0.0021 0.0256 0.0006 0 0.0002 0 0.0002 0.0017 0.0002 0.0001
Eisosomes 0 0 0 0 0.0001 0 0.0003 0 0 0 0 0 0 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0013 0 0.0003 0.0002 0.0006 0.0002 0.0031 0 0.0004 0.0003 0.0007 0 0 0 0.0018 0.0019 0.0005 0.0001
Endosome 0.0069 0 0.0071 0.0035 0.0151 0.0015 0.0079 0 0.0168 0.0018 0.0101 0.0001 0.0002 0 0.0016 0.0044 0.0009 0.0019
Golgi 0.0014 0 0.0014 0.0072 0.0081 0.001 0.0037 0 0.0089 0.0018 0.0009 0.0001 0.0002 0 0.0003 0.0006 0.0001 0.0004
Lipid Particles 0.0079 0 0.0031 0.01 0.0303 0.0034 0.0023 0 0.0004 0.0029 0.0003 0 0.0002 0 0.0005 0.0046 0.0002 0.0005
Mitochondria 0.0046 0.0001 0.0074 0.0082 0.032 0.0024 0.0035 0.0001 0.0034 0.001 0.0036 0.0188 0.0003 0.0002 0.0007 0.0017 0.0017 0.0006
None 0.0008 0 0.001 0.0103 0.0006 0.0027 0.0036 0 0.0002 0.001 0.0004 0 0.0001 0 0.001 0.0034 0.0006 0
Nuclear Periphery 0.0083 0.0004 0.0029 0.0296 0.0095 0.0048 0.0282 0.0003 0.0039 0.0327 0.0021 0.0011 0.0009 0.0004 0.0029 0.0051 0.0238 0.0007
Nucleolus 0.0364 0.0454 0.0224 0.0508 0.1753 0.0962 0.0378 0.0204 0.0318 0.0251 0.2974 0.0853 0.0365 0.031 0.0215 0.0232 0.1937 0.0989
Nucleus 0.8938 0.9526 0.9281 0.832 0.671 0.875 0.7971 0.9781 0.9072 0.8539 0.581 0.8886 0.9487 0.9675 0.9618 0.9438 0.7185 0.8831
Peroxisomes 0.007 0 0.0065 0.0241 0.0159 0.0008 0.0058 0 0.0006 0.0326 0.0002 0 0.0005 0 0.0001 0.0005 0.0001 0.0001
Punctate Nuclear 0.0165 0.0012 0.0053 0.0086 0.0185 0.0092 0.0294 0.0009 0.0071 0.016 0.0012 0.003 0.0018 0.0006 0.004 0.0033 0.0544 0.008
Vacuole 0.0015 0 0.0008 0.0002 0.0057 0.0001 0.0022 0 0.0009 0.0002 0.0419 0.0001 0.0002 0 0.0004 0.001 0.0005 0.0026
Vacuole Periphery 0.0011 0 0.0005 0.0004 0.0032 0.0002 0.0024 0 0.0006 0.0006 0.0097 0.0004 0.0001 0 0.0003 0.0006 0.0005 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.4861 8.7678 9.7591 13.5479 19.0748 4.9013 14.9599 9.7229 10.541 11.6021
Translational Efficiency 1.9635 2.4093 1.9738 1.5385 1.2917 7.6759 1.459 2.1482 2.6819 2.3612

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
494 1521 272 917 1809 241 1980 1328 2303 1762 2252 2245

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 736.19 848.41 1121.86 1051.65 740.76 958.57 992.37 982.21 739.78 863.48 1008.01 1010.57
Standard Deviation 106.84 162.74 152.82 251.10 140.84 149.18 119.51 140.02 134.29 165.34 130.99 196.25
Intensity Change Log 2 — 0.204683 0.607743 0.514505 — 0.371878 0.421872 0.407025 — 0.290960 0.517511 0.461599

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000404 0.000803 0.001854 0.002427 0.000511 0.001008 0.000972 0.001348 0.000488 0.000831 0.001078 0.001789
Bud Neck 0.002399 0.010708 0.012849 0.041452 0.004144 0.008624 0.033155 0.034974 0.003770 0.010423 0.030703 0.037620
Bud Site 0.000208 0.001256 0.001610 0.020780 0.000618 0.001610 0.001952 0.010458 0.000530 0.001304 0.001911 0.014674
Cell Periphery 0.000037 0.000092 0.000049 0.000491 0.000081 0.000078 0.000048 0.000208 0.000071 0.000090 0.000048 0.000324
Cytoplasm 0.000796 0.003206 0.000349 0.006191 0.001978 0.002259 0.000501 0.010427 0.001725 0.003077 0.000483 0.008696
Cytoplasmic Foci 0.000107 0.001079 0.000088 0.002612 0.000251 0.002884 0.000028 0.003369 0.000220 0.001326 0.000035 0.003060
Eisosomes 0.000022 0.000033 0.000062 0.000125 0.000022 0.000035 0.000040 0.000041 0.000022 0.000033 0.000043 0.000076
Endoplasmic Reticulum 0.001960 0.003166 0.004722 0.005184 0.002262 0.004197 0.003441 0.005652 0.002197 0.003307 0.003596 0.005461
Endosome 0.000071 0.000594 0.000168 0.003188 0.000209 0.003165 0.000149 0.005135 0.000179 0.000945 0.000152 0.004340
Golgi 0.000108 0.000365 0.000203 0.002002 0.000249 0.002137 0.000131 0.006383 0.000218 0.000607 0.000140 0.004593
Lipid Particles 0.000247 0.000234 0.000204 0.000458 0.000196 0.000594 0.000072 0.001020 0.000207 0.000284 0.000088 0.000790
Mitochondria 0.000845 0.002186 0.001867 0.096003 0.001570 0.007116 0.002556 0.007424 0.001414 0.002860 0.002473 0.043605
Mitotic Spindle 0.000587 0.005261 0.000502 0.042415 0.000862 0.029890 0.004161 0.029856 0.000803 0.008630 0.003719 0.034986
None 0.005980 0.007502 0.001185 0.009312 0.009462 0.002952 0.001358 0.008066 0.008715 0.006880 0.001337 0.008575
Nuclear Periphery 0.000194 0.000571 0.000257 0.002478 0.000236 0.004122 0.000793 0.002325 0.000227 0.001057 0.000728 0.002387
Nuclear Periphery Foci 0.000227 0.000897 0.000190 0.001152 0.000634 0.003304 0.000191 0.001185 0.000547 0.001227 0.000191 0.001171
Nucleolus 0.096541 0.100622 0.070347 0.031797 0.098207 0.100093 0.044837 0.029475 0.097850 0.100549 0.047918 0.030423
Nucleus 0.888366* 0.859185* 0.899645* 0.714441* 0.877643* 0.821578* 0.903864* 0.829473* 0.879943* 0.854041* 0.903354* 0.782487*
Peroxisomes 0.000717 0.000943 0.002119 0.002911 0.000545 0.001868 0.000428 0.000724 0.000582 0.001070 0.000633 0.001617
Vacuole 0.000094 0.000717 0.001507 0.010216 0.000170 0.001269 0.001013 0.008842 0.000153 0.000792 0.001072 0.009404
Vacuole Periphery 0.000090 0.000579 0.000226 0.004364 0.000152 0.001218 0.000310 0.003617 0.000139 0.000666 0.000300 0.003922

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.06 -10.74 -12.97 -10.75 -2.00 -2.36 -7.33 -11.14 -2.26 -6.05 -4.55 -10.41 -15.52 -13.41 -8.31
Bud Neck -12.70 -7.12 -19.25 -15.44 -11.05 -4.68 -22.93 -20.39 -15.28 -1.37 -12.11 -23.83 -27.74 -21.74 -4.88
Bud Site -4.77 -8.08 -7.53 -6.94 -6.37 -2.46 -6.10 -6.49 -4.99 -4.80 -2.92 -7.70 -9.74 -8.94 -7.91
Cell Periphery -3.17 -3.10 -5.20 -3.92 -4.54 -0.06 3.06 -2.88 -1.58 -4.69 -1.22 2.28 -5.50 -4.13 -6.46
Cytoplasm -2.90 0.96 -7.65 -2.61 -8.41 -0.44 2.81 -4.88 -3.87 -6.22 -1.75 2.90 -6.54 -4.14 -8.55
Cytoplasmic Foci -2.29 0.57 -3.66 0.10 -3.87 -1.35 2.18 -3.05 0.69 -3.76 -2.46 2.32 -4.12 0.09 -5.12
Eisosomes -5.18 -11.26 -7.68 -6.48 -1.71 -3.95 -18.12 -15.36 -2.27 -2.34 -9.09 -20.81 -12.05 -8.36 -4.53
Endoplasmic Reticulum -6.29 -7.69 -11.08 -7.93 -1.91 -2.72 -5.59 -14.68 -2.28 -7.91 -6.46 -7.34 -18.08 -11.12 -8.36
Endosome -2.52 -5.84 -4.76 -2.46 -4.28 -1.35 0.57 -3.99 0.33 -4.04 -2.26 -0.41 -5.44 -2.18 -5.42
Golgi -3.43 -5.38 -3.24 -2.11 -2.70 -1.66 0.63 -2.33 -0.31 -2.41 -2.23 0.31 -2.85 -2.10 -2.92
Lipid Particles 0.08 0.06 -0.96 -2.26 -2.49 -2.23 1.78 -1.46 0.35 -1.92 -1.12 1.70 -1.88 -1.27 -2.68
Mitochondria -3.55 -3.43 -6.91 -6.65 -6.68 -1.67 -1.92 -3.10 0.57 -2.38 -2.17 -2.58 -7.32 -6.64 -6.92
Mitotic Spindle -3.35 -1.09 -9.29 -7.98 -9.05 -3.30 -3.91 -8.76 -0.09 -7.28 -4.62 -4.07 -12.71 -8.49 -10.92
None -0.64 3.46 -0.30 0.43 -5.71 4.54 7.27 2.92 -2.37 -6.20 1.44 7.98 2.45 0.60 -8.24
Nuclear Periphery -2.55 -1.96 -10.80 -8.50 -10.33 -1.51 -2.31 -7.70 0.62 -4.70 -2.27 -2.41 -12.42 -4.16 -7.40
Nuclear Periphery Foci -3.27 -0.83 -5.14 -0.17 -4.51 -1.69 2.99 -1.46 1.41 -3.31 -2.36 2.85 -2.50 0.72 -4.88
Nucleolus -0.61 2.70 8.51 13.08 4.09 -0.54 12.03 17.86 7.69 6.05 -0.79 12.42 19.44 17.99 6.22
Nucleus 4.03 -0.09 11.53 9.56 10.97 3.92 -2.11 6.04 -1.18 7.51 5.30 -1.88 12.36 7.02 13.60
Peroxisomes -0.93 -4.85 -2.24 -1.89 2.17 -2.22 0.39 -0.50 2.16 -1.14 -3.37 -1.51 -2.75 0.88 -2.02
Vacuole -2.41 -6.18 -6.28 -5.85 -5.00 -2.76 -5.08 -7.42 -5.90 -6.44 -2.84 -6.38 -9.66 -8.75 -8.34
Vacuole Periphery -2.93 -7.17 -3.76 -2.53 -3.38 -1.60 -3.13 -2.48 -0.42 -2.25 -3.09 -3.91 -3.98 -2.38 -3.61
Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; component of the Middle domain of mediator; required for regulation of RNA polymerase II activity; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Cse2

Cse2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cse2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available