Standard name | |
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Human Ortholog | |
Description | Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; component of the Middle domain of mediator; required for regulation of RNA polymerase II activity; relocalizes to the cytosol in response to hypoxia |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | – | 6 | 1 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 3 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 1 | 0 | 0 | 1 | 0 | 6 | – | 11 | 9 | 11 | 15 | 16 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 1 | 3 | 5 | 15 | 6 | – | 11 | 9 | 8 | 3 | 13 | 13 | 23 | 47 | 31 | 28 | 21 | 0 | 0 | 0 | 0 | 3 | 2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 2 | 5 | 9 | 3 | 5 | 2 | 1 | 0 | 0 | 1 | 3 | 10 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 5 | 4 |
Mitochondria | 36 | 5 | 46 | 68 | 41 | 110 | – | 245 | 162 | 149 | 149 | 245 | 7 | 6 | 1 | 17 | 17 | 9 | 2 | 1 | 0 | 0 | 2 | 4 |
Nucleus | 309 | 49 | 74 | 166 | 236 | 229 | – | 402 | 202 | 270 | 220 | 354 | 191 | 178 | 259 | 260 | 272 | 153 | 300 | 62 | 81 | 66 | 189 | 228 |
Nuclear Periphery | 2 | 1 | 0 | 2 | 2 | 7 | – | 18 | 7 | 9 | 9 | 6 | 1 | 1 | 1 | 5 | 5 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 58 | 14 | 5 | 37 | 33 | 68 | – | 146 | 108 | 174 | 110 | 233 | 45 | 45 | 71 | 41 | 47 | 33 | 25 | 4 | 3 | 18 | 41 | 43 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 2 | – | 2 | 0 | 0 | 1 | 3 | 1 | 1 | 7 | 2 | 5 | 0 | 1 | 0 | 0 | 2 | 3 | 7 |
Vac/Vac Membrane | 2 | 0 | 0 | 3 | 1 | 2 | – | 3 | 2 | 2 | 3 | 7 | 3 | 3 | 3 | 4 | 14 | 6 | 6 | 1 | 1 | 3 | 17 | 24 |
Unique Cell Count | 332 | 56 | 82 | 200 | 270 | 297 | 586 | 317 | 421 | 325 | 576 | 226 | 225 | 348 | 334 | 353 | 202 | 341 | 73 | 89 | 98 | 277 | 337 | |
Labelled Cell Count | 411 | 70 | 128 | 282 | 329 | 431 | 845 | 500 | 626 | 512 | 881 | 263 | 264 | 404 | 370 | 401 | 229 | 341 | 73 | 89 | 98 | 277 | 337 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.7 | 6.7 | 4.3 | 4.9 | 5.5 | 4.7 | – | 4.7 | 4.2 | 4.8 | 4.6 | 4.7 | 7.3 | 7.6 | 7.1 | 10.2 | 10.1 | 9.2 | 6.3 | 7.5 | 7.9 |
Std Deviation (1e-4) | 1.3 | 1.1 | 1.1 | 1.2 | 1.5 | 1.1 | – | 1.3 | 1.1 | 1.4 | 1.3 | 1.4 | 2.0 | 2.6 | 2.3 | 2.6 | 2.5 | 2.2 | 1.6 | 2.1 | 2.1 |
Intensity Change (Log2) | – | – | – | 0.2 | 0.38 | 0.12 | – | 0.14 | -0.01 | 0.16 | 0.09 | 0.12 | 0.77 | 0.83 | 0.73 | 1.25 | 1.24 | 1.1 | 0.56 | 0.8 | 0.88 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1.8 | 2.5 | 1.7 | 1.4 | 1.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -3.4 | -7.5 | 0 | – | 0 | 0 | 0 | 0 | 0 | -10.9 | -11.0 | -14.6 | -11.5 | -11.9 | -10.0 |
Nucleus | -1.6 | -0.7 | -2.6 | – | -4.1 | -4.6 | -4.7 | -4.1 | -5.1 | -1.3 | -2.3 | -3.1 | -2.5 | -2.7 | -2.8 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2.7 | 1.6 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.9 | 2.9 | 3.1 | 1.6 | 1.8 | 2.3 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.6769 | 2.1845 | 2.0149 | 1.3312 | 1.9612 | 1.8685 | 1.4749 | 1.9106 | 1.6096 | 1.0432 | 1.933 | 1.2667 | 3.2508 | 3.9104 | 3.3841 | 3.3269 | 3.3459 | 3.2902 |
Actin | 0.0045 | 0 | 0.0027 | 0.0025 | 0.0016 | 0.0007 | 0.047 | 0 | 0.004 | 0.0025 | 0.0006 | 0.0008 | 0.0096 | 0 | 0.0015 | 0.0021 | 0.0009 | 0.0001 |
Bud | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0006 | 0 | 0.0005 | 0 | 0.0014 | 0.0001 | 0.0123 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0003 | 0.0001 | 0.001 | 0.0003 | 0.0016 | 0.001 | 0.0006 | 0 | 0.0006 | 0.0004 | 0.0009 | 0.0009 | 0.0002 | 0.0001 | 0.0004 | 0.0003 | 0.0012 | 0.0017 |
Bud Periphery | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0026 | 0.0001 | 0.0008 | 0 | 0.0019 | 0.0001 | 0.0272 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0003 | 0.0002 |
Bud Site | 0.0004 | 0.0001 | 0.0029 | 0.0002 | 0.0016 | 0.0001 | 0.0015 | 0 | 0.0052 | 0.0003 | 0.0063 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0006 | 0.0004 | 0.0002 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0 | 0.0006 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0008 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.0008 | 0 | 0.0007 | 0.0046 | 0.0004 | 0.0003 | 0.0126 | 0 | 0.0025 | 0.001 | 0.0019 | 0 | 0.0002 | 0 | 0.0004 | 0.0007 | 0.0012 | 0.0001 |
Cytoplasmic Foci | 0.0061 | 0 | 0.005 | 0.0071 | 0.0052 | 0.0003 | 0.0095 | 0 | 0.0021 | 0.0256 | 0.0006 | 0 | 0.0002 | 0 | 0.0002 | 0.0017 | 0.0002 | 0.0001 |
Eisosomes | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0013 | 0 | 0.0003 | 0.0002 | 0.0006 | 0.0002 | 0.0031 | 0 | 0.0004 | 0.0003 | 0.0007 | 0 | 0 | 0 | 0.0018 | 0.0019 | 0.0005 | 0.0001 |
Endosome | 0.0069 | 0 | 0.0071 | 0.0035 | 0.0151 | 0.0015 | 0.0079 | 0 | 0.0168 | 0.0018 | 0.0101 | 0.0001 | 0.0002 | 0 | 0.0016 | 0.0044 | 0.0009 | 0.0019 |
Golgi | 0.0014 | 0 | 0.0014 | 0.0072 | 0.0081 | 0.001 | 0.0037 | 0 | 0.0089 | 0.0018 | 0.0009 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0006 | 0.0001 | 0.0004 |
Lipid Particles | 0.0079 | 0 | 0.0031 | 0.01 | 0.0303 | 0.0034 | 0.0023 | 0 | 0.0004 | 0.0029 | 0.0003 | 0 | 0.0002 | 0 | 0.0005 | 0.0046 | 0.0002 | 0.0005 |
Mitochondria | 0.0046 | 0.0001 | 0.0074 | 0.0082 | 0.032 | 0.0024 | 0.0035 | 0.0001 | 0.0034 | 0.001 | 0.0036 | 0.0188 | 0.0003 | 0.0002 | 0.0007 | 0.0017 | 0.0017 | 0.0006 |
None | 0.0008 | 0 | 0.001 | 0.0103 | 0.0006 | 0.0027 | 0.0036 | 0 | 0.0002 | 0.001 | 0.0004 | 0 | 0.0001 | 0 | 0.001 | 0.0034 | 0.0006 | 0 |
Nuclear Periphery | 0.0083 | 0.0004 | 0.0029 | 0.0296 | 0.0095 | 0.0048 | 0.0282 | 0.0003 | 0.0039 | 0.0327 | 0.0021 | 0.0011 | 0.0009 | 0.0004 | 0.0029 | 0.0051 | 0.0238 | 0.0007 |
Nucleolus | 0.0364 | 0.0454 | 0.0224 | 0.0508 | 0.1753 | 0.0962 | 0.0378 | 0.0204 | 0.0318 | 0.0251 | 0.2974 | 0.0853 | 0.0365 | 0.031 | 0.0215 | 0.0232 | 0.1937 | 0.0989 |
Nucleus | 0.8938 | 0.9526 | 0.9281 | 0.832 | 0.671 | 0.875 | 0.7971 | 0.9781 | 0.9072 | 0.8539 | 0.581 | 0.8886 | 0.9487 | 0.9675 | 0.9618 | 0.9438 | 0.7185 | 0.8831 |
Peroxisomes | 0.007 | 0 | 0.0065 | 0.0241 | 0.0159 | 0.0008 | 0.0058 | 0 | 0.0006 | 0.0326 | 0.0002 | 0 | 0.0005 | 0 | 0.0001 | 0.0005 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.0165 | 0.0012 | 0.0053 | 0.0086 | 0.0185 | 0.0092 | 0.0294 | 0.0009 | 0.0071 | 0.016 | 0.0012 | 0.003 | 0.0018 | 0.0006 | 0.004 | 0.0033 | 0.0544 | 0.008 |
Vacuole | 0.0015 | 0 | 0.0008 | 0.0002 | 0.0057 | 0.0001 | 0.0022 | 0 | 0.0009 | 0.0002 | 0.0419 | 0.0001 | 0.0002 | 0 | 0.0004 | 0.001 | 0.0005 | 0.0026 |
Vacuole Periphery | 0.0011 | 0 | 0.0005 | 0.0004 | 0.0032 | 0.0002 | 0.0024 | 0 | 0.0006 | 0.0006 | 0.0097 | 0.0004 | 0.0001 | 0 | 0.0003 | 0.0006 | 0.0005 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.4861 | 8.7678 | 9.7591 | 13.5479 | 19.0748 | 4.9013 | 14.9599 | 9.7229 | 10.541 | 11.6021 |
Translational Efficiency | 1.9635 | 2.4093 | 1.9738 | 1.5385 | 1.2917 | 7.6759 | 1.459 | 2.1482 | 2.6819 | 2.3612 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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