Standard name
Human Ortholog
Description Uridine/cytidine kinase; component of the pyrimidine ribonucleotide salvage pathway that converts uridine into UMP and cytidine into CMP; involved in the pyrimidine deoxyribonucleotide salvage pathway, converting deoxycytidine into dCMP

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.35 0.3 0.36 0.31 0.47 0.63 0.66 0.65 0.64 0.64 0.55 0.59 0.35 0.4 0.51 0.5 0.6 0.25 0.2 0.26 0.19 0.2 0.22
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0.05 0 0.08 0.06 0.33 0.36 0.36 0.5 0.5 0.59 0.6 0 0 0 0.05 0 0 0 0 0 0 0
Nucleus 0.26 0.22 0.25 0.31 0.21 0.06 0 0.05 0.06 0 0 0 0.29 0.16 0.11 0.24 0.19 0.5 0.6 0.42 0.55 0.52 0.36
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Vac/Vac Membrane 0.44 0.7 0.48 0.41 0.37 0.15 0.16 0.11 0.05 0.07 0.06 0.06 0.47 0.6 0.51 0.35 0.35 0.12 0.12 0.17 0.1 0.1 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0
Bud 1 0 0 0 0 1 1 0 0 6 4 3 0 0 0 0 0 6 8 11 5 13 18
Bud Neck 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 1 0 0 1 5
Bud Site 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 2 1 3 0 2 2 0 0 0 1 1 0 0 0 0 0 0
Cytoplasm 46 64 66 52 103 236 321 474 229 358 217 307 81 132 201 95 71 39 48 60 22 52 67
Endoplasmic Reticulum 0 0 1 0 3 0 0 0 3 2 0 1 1 0 0 6 5 1 1 1 1 2 4
Endosome 0 0 1 3 3 3 3 2 0 0 0 0 2 2 7 3 2 4 1 5 0 6 8
Golgi 0 0 1 0 0 2 0 0 0 0 0 0 0 0 0 0 1 0 0 2 0 0 1
Mitochondria 21 10 8 14 13 123 178 258 177 279 235 310 2 2 1 10 5 0 1 1 2 1 1
Nucleus 34 48 45 51 46 24 14 34 22 22 16 15 66 53 45 46 23 78 146 96 63 138 107
Nuclear Periphery 0 0 0 0 1 0 0 6 2 5 2 2 0 0 0 0 2 0 0 1 0 0 2
Nucleolus 0 0 1 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 1 0 4 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 2 1 5 3 12 7
Vac/Vac Membrane 59 152 86 68 81 56 77 77 19 38 23 32 108 197 203 66 41 19 29 39 10 26 66
Unique Cell Count 133 216 181 167 218 373 490 725 356 559 398 517 231 331 395 189 118 157 243 232 115 265 302
Labelled Cell Count 161 274 209 188 250 445 597 854 457 710 500 673 260 386 457 228 151 157 243 232 115 265 302


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 6.1 5.4 5.8 5.4 4.5 3.8 4.0 3.7 3.6 3.6 3.6 7.1 6.5 6.1 6.7 7.6 6.8 6.8 7.2
Std Deviation (1e-4) 0.8 0.9 0.8 1.2 1.1 1.0 0.7 0.8 0.6 1.0 0.6 1.0 1.3 1.2 1.2 1.1 1.5 1.3 1.4 1.4
Intensity Change (Log2) 0.11 -0.0 -0.28 -0.51 -0.42 -0.54 -0.61 -0.61 -0.61 0.4 0.27 0.18 0.31 0.48 0.33 0.33 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.0 2.2 5.9 6.8 7.1 6.1 6.5 4.0 5.3 -0.3 0.8 3.2 2.7 4.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.5 0.7 0 0 0 0 0 0 0 0 0 0 0.4 -0.1
Nucleus 1.2 -0.9 -6.2 -8.9 -8.6 -6.2 -8.5 -7.6 -9.1 0.8 -2.4 -4.1 -0.1 -1.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.3 -2.1 -8.2 -8.5 -11.6 -11.6 -12.7 -11.9 -12.8 -0.2 2.6 0.9 -2.5 -2.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.571 1.6187 1.3748 1.0985 1.1314 1.454 0.1857 0.7058 0.4014 0.3696 0.5679 0.5285 1.2566 1.703 1.1667 1.6098 0.7501 1.2967
Actin 0.0259 0.0005 0.0125 0.0104 0.0026 0.0006 0.0342 0.0011 0.0032 0.0152 0.0139 0.0023 0.0194 0.0003 0.0296 0.0015 0.0032 0.0003
Bud 0.0056 0.0026 0.0006 0.0003 0.0008 0.0002 0.0023 0.0011 0.0003 0.0009 0.0003 0.0003 0.002 0.0001 0.0061 0.0003 0.0002 0.0007
Bud Neck 0.0024 0.0007 0.0007 0.0041 0.0009 0.0036 0.0036 0.0006 0.0004 0.0073 0.0023 0.0026 0.0259 0.0002 0.0007 0.0011 0.0007 0.0011
Bud Periphery 0.0033 0.0008 0.0023 0.0004 0.0021 0.0002 0.0036 0.0008 0.0004 0.0013 0.0003 0.0009 0.0039 0 0.0065 0.0002 0.0003 0.0002
Bud Site 0.0142 0.0148 0.0018 0.0017 0.0015 0.0003 0.0131 0.0244 0.001 0.0077 0.0024 0.0004 0.052 0.0008 0.0073 0.0103 0.0003 0.0002
Cell Periphery 0.0005 0.0002 0.0002 0.0002 0.0003 0.0001 0.0007 0.0001 0.0001 0.0012 0.0001 0.0001 0.0024 0.0001 0.0002 0.0001 0.0001 0.0001
Cytoplasm 0.2505 0.4485 0.3025 0.3568 0.3286 0.3238 0.2388 0.356 0.27 0.1511 0.3643 0.2979 0.392 0.3193 0.3114 0.2971 0.3607 0.44
Cytoplasmic Foci 0.033 0.0125 0.0105 0.0099 0.018 0.0066 0.0351 0.0094 0.0068 0.0225 0.0413 0.0093 0.0103 0.0025 0.0145 0.0297 0.0114 0.0119
Eisosomes 0.0005 0.0002 0.0002 0.0002 0.0003 0 0.0009 0.0001 0.0001 0.0001 0.0001 0.0001 0.0019 0.0001 0.0002 0 0.0001 0
Endoplasmic Reticulum 0.009 0.0043 0.0056 0.0042 0.0049 0.0056 0.0117 0.0056 0.0045 0.0032 0.0088 0.003 0.009 0.007 0.0041 0.0024 0.0071 0.0037
Endosome 0.0333 0.0028 0.0121 0.0053 0.0089 0.0087 0.027 0.0028 0.0041 0.0385 0.0231 0.004 0.0137 0.0032 0.0049 0.004 0.0256 0.0094
Golgi 0.0132 0.0003 0.0033 0.0054 0.0006 0.0002 0.0109 0.0003 0.0014 0.0208 0.0084 0.0008 0.0044 0.0001 0.0067 0.004 0.0274 0.0011
Lipid Particles 0.0397 0.0008 0.0042 0.002 0.0003 0.0004 0.0273 0.0003 0.0003 0.009 0.0131 0.0035 0.0045 0.0001 0.0025 0.0075 0.0011 0.0046
Mitochondria 0.0084 0.0007 0.007 0.0113 0.0536 0.0012 0.0078 0.0005 0.0109 0.0057 0.0012 0.0064 0.026 0.0003 0.0033 0.0034 0.0367 0.0011
None 0.2516 0.2666 0.3393 0.2115 0.2873 0.2288 0.3591 0.3601 0.3688 0.4473 0.1401 0.4044 0.2938 0.2671 0.2773 0.3034 0.3049 0.2535
Nuclear Periphery 0.016 0.0056 0.0105 0.0116 0.0101 0.0111 0.0164 0.012 0.0123 0.0052 0.0122 0.0045 0.0113 0.0228 0.0124 0.0054 0.0135 0.0049
Nucleolus 0.0041 0.0013 0.0021 0.0021 0.0012 0.0024 0.0028 0.001 0.0011 0.0021 0.0013 0.0011 0.0015 0.0009 0.0013 0.0007 0.0005 0.0005
Nucleus 0.2203 0.2172 0.2601 0.3427 0.2654 0.3794 0.1423 0.2015 0.3025 0.2096 0.3264 0.2451 0.1075 0.366 0.2809 0.293 0.1657 0.2532
Peroxisomes 0.0319 0.001 0.0043 0.0037 0.0005 0.0002 0.0279 0.0008 0.0004 0.0116 0.025 0.0029 0.002 0.0001 0.0194 0.0316 0.0007 0.0002
Punctate Nuclear 0.0288 0.0165 0.0139 0.0141 0.0087 0.0227 0.0273 0.0191 0.0095 0.0232 0.0115 0.0087 0.0075 0.0067 0.0086 0.0034 0.0368 0.0113
Vacuole 0.0059 0.0019 0.0055 0.0016 0.0029 0.0033 0.0059 0.0022 0.0015 0.0151 0.0035 0.0014 0.0079 0.0019 0.0016 0.0007 0.0022 0.0015
Vacuole Periphery 0.0021 0.0002 0.0009 0.0004 0.0005 0.0006 0.0014 0.0003 0.0004 0.0013 0.0006 0.0003 0.0011 0.0003 0.0005 0.0002 0.001 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.8812 15.7191 16.5244 15.8636 16.624 16.8421 17.3836 10.7271 10.2657 19.5931
Translational Efficiency 1.1628 1.2474 0.9329 0.726 1.0431 0.8626 0.8024 0.8479 0.8846 0.7519

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Uridine/cytidine kinase; component of the pyrimidine ribonucleotide salvage pathway that converts uridine into UMP and cytidine into CMP; involved in the pyrimidine deoxyribonucleotide salvage pathway, converting deoxycytidine into dCMP
Localization
Cell Percentages cytoplasm (31%), nucleus (16%), mixed (52%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Urk1

Urk1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Urk1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available