Standard name | |
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Human Ortholog | |
Description | Low-affinity vacuolar phosphate transporter; exports phosphate from the vacuolar lumen to the cytosol; regulates phosphate and polyphosphate metabolism; acts upstream of Pho81p in regulation of the PHO pathway; localizes to sites of contact between the vacuole and mitochondria (vCLAMPs); deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 3 | 17 | 28 | 5 | 1 | 0 | 4 | – | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 3 | 2 | 7 | 4 | 5 | 4 |
Bud | 0 | 7 | 2 | 14 | 6 | 7 | 9 | 8 | – | 11 | 10 | 14 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 1 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 3 | 5 | 12 | 4 | 3 | 2 | 0 | – | 5 | 4 | 1 | 7 | 12 | 4 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 245 | 112 | 188 | 166 | 306 | 491 | 523 | 468 | – | 450 | 315 | 374 | 29 | 66 | 130 | 344 | 216 | 113 | 15 | 16 | 8 | 4 | 12 | 26 |
Endoplasmic Reticulum | 23 | 67 | 181 | 97 | 21 | 13 | 6 | 3 | – | 3 | 11 | 2 | 40 | 29 | 20 | 11 | 3 | 2 | 249 | 142 | 339 | 135 | 116 | 138 |
Endosome | 9 | 0 | 18 | 18 | 6 | 1 | 2 | 11 | – | 1 | 0 | 4 | 0 | 0 | 0 | 0 | 2 | 1 | 6 | 3 | 5 | 1 | 0 | 5 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 1 | 2 | 1 | 0 | 1 |
Mitochondria | 0 | 4 | 0 | 3 | 3 | 23 | 27 | 25 | – | 87 | 74 | 78 | 0 | 0 | 0 | 9 | 4 | 2 | 5 | 6 | 7 | 9 | 6 | 7 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 2 | – | 1 | 0 | 0 | 0 | 0 | 0 | 4 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 1 | – | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 1 | 2 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 |
Vac/Vac Membrane | 36 | 4 | 17 | 15 | 25 | 22 | 23 | 6 | – | 14 | 7 | 9 | 0 | 1 | 1 | 34 | 37 | 25 | 64 | 31 | 34 | 16 | 24 | 33 |
Unique Cell Count | 299 | 182 | 378 | 296 | 356 | 529 | 557 | 501 | 502 | 363 | 424 | 74 | 97 | 149 | 377 | 247 | 132 | 356 | 209 | 418 | 181 | 172 | 226 | |
Labelled Cell Count | 317 | 200 | 432 | 354 | 376 | 562 | 597 | 528 | 572 | 425 | 487 | 77 | 109 | 156 | 405 | 268 | 146 | 356 | 209 | 418 | 181 | 172 | 226 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.4 | 7.5 | 7.5 | 7.5 | 6.3 | 4.8 | 4.4 | 4.7 | – | 3.8 | 3.8 | 3.9 | 8.4 | 7.7 | 7.2 | 6.0 | 6.6 | 7.1 | 9.4 | 8.9 | 9.2 |
Std Deviation (1e-4) | 1.2 | 1.1 | 1.1 | 1.5 | 1.2 | 1.2 | 1.0 | 1.2 | – | 0.9 | 0.7 | 0.8 | 0.8 | 1.1 | 1.1 | 0.9 | 1.1 | 1.2 | 1.7 | 1.5 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.0 | -0.26 | -0.64 | -0.77 | -0.67 | – | -0.98 | -0.96 | -0.94 | 0.16 | 0.03 | -0.06 | -0.33 | -0.18 | -0.07 | 0.33 | 0.25 | 0.3 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 2.6 | -2.5 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -2.2 | -3.8 | -3.4 | 0 |
Bud | 3.6 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 2.2 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.6 | 10.5 | 14.7 | 15.5 | 14.7 | – | 13.1 | 10.8 | 11.9 | -1.7 | 3.2 | 7.9 | 12.5 | 9.6 | 7.2 |
Endoplasmic Reticulum | -4.0 | -12.7 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1.0 | -3.2 | -7.3 | -14.2 | -12.5 | -9.6 |
Endosome | 0.8 | -2.3 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -2.7 | -4.3 | -2.7 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.3 | 1.5 | -0.2 | -0.3 | -3.0 | – | -1.4 | -2.0 | -1.9 | 0 | 0 | -2.2 | 2.5 | 4.6 | 5.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.2896 | 3.1465 | 3.4577 | 3.924 | 2.9427 | 3.0563 | 6.9683 | 6.8676 | 6.9123 | 6.4277 | 5.4687 | 5.9332 | 6.18 | 6.9158 | 6.4691 | 6.415 | 5.7654 | 6.6635 |
Actin | 0.0125 | 0.0017 | 0.0116 | 0.0399 | 0.0377 | 0.0028 | 0.0507 | 0.0011 | 0.0744 | 0.0025 | 0.0739 | 0.0013 | 0.0006 | 0.0011 | 0.0028 | 0.0011 | 0.0008 | 0.0006 |
Bud | 0.001 | 0.0002 | 0.0003 | 0.001 | 0.0004 | 0.0002 | 0.0009 | 0 | 0.0027 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0081 | 0.0004 | 0.0021 | 0.001 | 0.0024 | 0.0009 | 0.001 | 0.0003 | 0.0015 | 0.0035 | 0.0013 | 0.0008 | 0.0002 | 0.0006 | 0.0004 | 0.001 | 0.0008 | 0.0029 |
Bud Periphery | 0.0028 | 0.0002 | 0.0003 | 0.0013 | 0.0005 | 0.0003 | 0.0012 | 0.0001 | 0.0035 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Bud Site | 0.0063 | 0.0039 | 0.0045 | 0.002 | 0.0087 | 0.0002 | 0.0054 | 0.0004 | 0.0086 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0025 | 0.0005 | 0.0003 | 0.0004 | 0.0001 |
Cell Periphery | 0.0055 | 0.0014 | 0.0012 | 0.0007 | 0.0006 | 0.0006 | 0.0026 | 0.0033 | 0.0021 | 0.0041 | 0.0013 | 0.0009 | 0.0026 | 0.0022 | 0.0016 | 0.0014 | 0.0009 | 0.0008 |
Cytoplasm | 0.2687 | 0.4774 | 0.4449 | 0.4399 | 0.4463 | 0.3911 | 0.2623 | 0.3234 | 0.2862 | 0.3162 | 0.4997 | 0.3797 | 0.2748 | 0.3528 | 0.3112 | 0.3078 | 0.3663 | 0.3213 |
Cytoplasmic Foci | 0.0277 | 0.0128 | 0.02 | 0.0284 | 0.0254 | 0.0166 | 0.0229 | 0.006 | 0.03 | 0.0164 | 0.0064 | 0.0081 | 0.0066 | 0.0111 | 0.0059 | 0.0098 | 0.0111 | 0.0064 |
Eisosomes | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0008 | 0 | 0.0007 | 0 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.4343 | 0.3535 | 0.37 | 0.3773 | 0.3658 | 0.4662 | 0.1634 | 0.6252 | 0.4137 | 0.5194 | 0.3175 | 0.4777 | 0.6317 | 0.5279 | 0.5893 | 0.5913 | 0.524 | 0.6066 |
Endosome | 0.0771 | 0.0364 | 0.0545 | 0.0389 | 0.029 | 0.0318 | 0.1034 | 0.006 | 0.0472 | 0.0305 | 0.0137 | 0.0268 | 0.0131 | 0.0184 | 0.0141 | 0.0231 | 0.0174 | 0.0101 |
Golgi | 0.0082 | 0.002 | 0.0055 | 0.0065 | 0.0039 | 0.0018 | 0.0088 | 0.0009 | 0.0302 | 0.0144 | 0.0018 | 0.0059 | 0.0013 | 0.0028 | 0.0018 | 0.001 | 0.0011 | 0.0007 |
Lipid Particles | 0.0111 | 0.0088 | 0.0127 | 0.0108 | 0.0046 | 0.0037 | 0.0328 | 0.0122 | 0.0144 | 0.0361 | 0.009 | 0.0063 | 0.0114 | 0.0088 | 0.0087 | 0.0091 | 0.0185 | 0.0047 |
Mitochondria | 0.003 | 0.0007 | 0.002 | 0.0135 | 0.0013 | 0.0008 | 0.0078 | 0.0001 | 0.0122 | 0.0033 | 0.0003 | 0.0082 | 0.0002 | 0.0004 | 0.0003 | 0.0005 | 0.0003 | 0.0003 |
None | 0.0457 | 0.0575 | 0.0232 | 0.0113 | 0.0529 | 0.0484 | 0.0688 | 0.0036 | 0.0261 | 0.0159 | 0.0208 | 0.0516 | 0.0383 | 0.0384 | 0.0415 | 0.0259 | 0.0183 | 0.0266 |
Nuclear Periphery | 0.0313 | 0.0175 | 0.0191 | 0.0106 | 0.0061 | 0.0163 | 0.135 | 0.0059 | 0.0075 | 0.0086 | 0.0232 | 0.0135 | 0.0089 | 0.0129 | 0.0103 | 0.0105 | 0.0203 | 0.0076 |
Nucleolus | 0.0029 | 0.0004 | 0.001 | 0.0001 | 0.0003 | 0.0003 | 0.0033 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.0001 |
Nucleus | 0.0182 | 0.0081 | 0.0098 | 0.003 | 0.0029 | 0.0044 | 0.0637 | 0.001 | 0.0023 | 0.0025 | 0.005 | 0.0045 | 0.002 | 0.0037 | 0.0023 | 0.0038 | 0.0078 | 0.0034 |
Peroxisomes | 0.0022 | 0.0001 | 0.0022 | 0.0031 | 0.0021 | 0.0002 | 0.0057 | 0.0001 | 0.0265 | 0.0099 | 0.0003 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0001 |
Punctate Nuclear | 0.0145 | 0.0021 | 0.003 | 0.0014 | 0.0016 | 0.0055 | 0.0341 | 0.0005 | 0.0018 | 0.0009 | 0.0007 | 0.0017 | 0.0004 | 0.0051 | 0.0006 | 0.0011 | 0.0029 | 0.0009 |
Vacuole | 0.0158 | 0.014 | 0.0105 | 0.0083 | 0.0064 | 0.0069 | 0.0171 | 0.0098 | 0.007 | 0.0145 | 0.0231 | 0.0113 | 0.007 | 0.0096 | 0.0077 | 0.0109 | 0.0081 | 0.0061 |
Vacuole Periphery | 0.0027 | 0.001 | 0.0013 | 0.0007 | 0.0004 | 0.0009 | 0.0081 | 0.0002 | 0.0014 | 0.0007 | 0.0014 | 0.0009 | 0.0003 | 0.0005 | 0.0004 | 0.0006 | 0.0005 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 41.0951 | 40.0428 | 43.6162 | 50.9132 | 38.3628 | 37.7866 | 45.8732 | 43.4963 | 39.3243 | 45.6409 |
Translational Efficiency | 0.7794 | 0.9172 | 0.8253 | 0.553 | 0.8314 | 0.8559 | 0.786 | 0.6491 | 0.8465 | 0.7767 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
333 | 1064 | 1947 | 579 | 1903 | 1987 | 1375 | 1501 | 2236 | 3051 | 3322 | 2080 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 891.38 | 833.02 | 1020.51 | 1074.64 | 844.67 | 896.06 | 1047.90 | 1174.23 | 851.63 | 874.08 | 1031.85 | 1146.51 |
Standard Deviation | 118.16 | 136.02 | 138.01 | 208.96 | 113.98 | 125.90 | 168.07 | 167.16 | 115.81 | 132.96 | 151.78 | 185.23 |
Intensity Change Log 2 | — | -0.097689 | 0.195178 | 0.269741 | — | 0.085208 | 0.311041 | 0.475255 | — | -0.005804 | 0.252714 | 0.373392 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.005313 | 0.001371 | 0.001496 | 0.005275 | 0.000416 | 0.000821 | 0.001740 | 0.003297 | 0.001145 | 0.001013 | 0.001597 | 0.003848 |
Bud Neck | 0.005543 | 0.004371 | 0.002024 | 0.010652 | 0.003371 | 0.005624 | 0.002616 | 0.008808 | 0.003694 | 0.005187 | 0.002269 | 0.009321 |
Bud Site | 0.024996 | 0.006569 | 0.005575 | 0.050936 | 0.002207 | 0.004950 | 0.006608 | 0.022142 | 0.005601 | 0.005515 | 0.006003 | 0.030157 |
Cell Periphery | 0.001144 | 0.000687 | 0.001076 | 0.002345 | 0.001599 | 0.000856 | 0.000965 | 0.003884 | 0.001531 | 0.000797 | 0.001030 | 0.003456 |
Cytoplasm | 0.153873* | 0.221733* | 0.109569* | 0.112967* | 0.212322* | 0.166472* | 0.135579* | 0.131807* | 0.203617* | 0.185744* | 0.120335* | 0.126563* |
Cytoplasmic Foci | 0.065921 | 0.104611 | 0.007733 | 0.044328 | 0.032943 | 0.053400 | 0.018935 | 0.022247 | 0.037854 | 0.071259 | 0.012369 | 0.028393 |
Eisosomes | 0.000383 | 0.000440 | 0.000217 | 0.000296 | 0.001541 | 0.000844 | 0.000312 | 0.000857 | 0.001368 | 0.000703 | 0.000256 | 0.000701 |
Endoplasmic Reticulum | 0.221347* | 0.397103* | 0.767962* | 0.395523* | 0.599751* | 0.549558* | 0.625973* | 0.562347* | 0.543397* | 0.496391* | 0.709192* | 0.515909* |
Endosome | 0.029748 | 0.028510 | 0.007290 | 0.044926 | 0.010265 | 0.025925 | 0.009108 | 0.026364 | 0.013167 | 0.026826 | 0.008043 | 0.031531 |
Golgi | 0.012278 | 0.013656 | 0.001419 | 0.015907 | 0.024745 | 0.019943 | 0.002192 | 0.008045 | 0.022888 | 0.017750 | 0.001739 | 0.010233 |
Lipid Particles | 0.023950 | 0.058921 | 0.009092 | 0.062155 | 0.049118 | 0.051517 | 0.031212 | 0.055266 | 0.045370 | 0.054099 | 0.018248 | 0.057183 |
Mitochondria | 0.007785 | 0.003578 | 0.000638 | 0.001440 | 0.004938 | 0.007694 | 0.001096 | 0.002127 | 0.005362 | 0.006259 | 0.000828 | 0.001936 |
Mitotic Spindle | 0.005360 | 0.001869 | 0.000393 | 0.005808 | 0.000041 | 0.000624 | 0.000624 | 0.003183 | 0.000833 | 0.001058 | 0.000488 | 0.003913 |
None | 0.000109 | 0.003308 | 0.001538 | 0.000813 | 0.004916 | 0.002126 | 0.004946 | 0.002471 | 0.004200 | 0.002538 | 0.002948 | 0.002010 |
Nuclear Periphery | 0.002881 | 0.007089 | 0.008629 | 0.001990 | 0.001404 | 0.002717 | 0.023396 | 0.001531 | 0.001624 | 0.004242 | 0.014741 | 0.001659 |
Nuclear Periphery Foci | 0.019151 | 0.018458 | 0.032068 | 0.046468 | 0.002815 | 0.003755 | 0.048241 | 0.022619 | 0.005248 | 0.008882 | 0.038762 | 0.029257 |
Nucleolus | 0.000153 | 0.000984 | 0.000064 | 0.000162 | 0.000236 | 0.000279 | 0.000389 | 0.000221 | 0.000223 | 0.000525 | 0.000199 | 0.000204 |
Nucleus | 0.022151 | 0.028319 | 0.021878 | 0.008871 | 0.016609 | 0.014339 | 0.042582 | 0.014266 | 0.017434 | 0.019214 | 0.030448 | 0.012764 |
Peroxisomes | 0.001357 | 0.003112 | 0.000320 | 0.001446 | 0.003322 | 0.003301 | 0.000788 | 0.001213 | 0.003030 | 0.003235 | 0.000514 | 0.001277 |
Vacuole | 0.392973* | 0.091508 | 0.020708 | 0.183359 | 0.026034 | 0.080784 | 0.042228 | 0.105122 | 0.080681 | 0.084524 | 0.029615 | 0.126901 |
Vacuole Periphery | 0.003584 | 0.003804 | 0.000312 | 0.004334 | 0.001408 | 0.004470 | 0.000470 | 0.002184 | 0.001732 | 0.004238 | 0.000377 | 0.002782 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.69 | 1.58 | -0.26 | -3.06 | -2.83 | -2.04 | -2.74 | -5.93 | -4.82 | -2.31 | 0.08 | -1.30 | -4.97 | -5.54 | -3.78 |
Bud Neck | 0.68 | 2.54 | -3.11 | -4.42 | -6.06 | -2.61 | 1.16 | -5.63 | -2.87 | -6.35 | -2.36 | 2.58 | -6.86 | -4.68 | -8.69 |
Bud Site | 4.03 | 4.22 | -3.87 | -7.84 | -7.98 | -3.77 | -3.64 | -9.93 | -8.32 | -6.67 | -0.17 | -0.83 | -11.12 | -11.31 | -10.70 |
Cell Periphery | 1.46 | -0.23 | -3.38 | -5.11 | -3.51 | 4.47 | 3.25 | -5.79 | -8.43 | -7.78 | 4.99 | 2.77 | -6.27 | -9.70 | -8.27 |
Cytoplasm | -5.59 | 4.15 | 2.59 | 9.52 | -1.27 | 7.40 | 10.34 | 11.39 | 4.62 | 0.06 | 3.17 | 15.45 | 12.11 | 9.66 | -2.14 |
Cytoplasmic Foci | -6.54 | 12.34 | 3.61 | 11.07 | -10.27 | -7.74 | 6.25 | 4.35 | 11.36 | -1.83 | -13.08 | 14.76 | 4.03 | 16.44 | -9.84 |
Eisosomes | -0.81 | 1.77 | 0.86 | 1.46 | -1.21 | 2.94 | 5.40 | 2.83 | 0.12 | -3.35 | 3.34 | 5.89 | 3.31 | 0.23 | -3.98 |
Endoplasmic Reticulum | -9.90 | -30.44 | -7.13 | 2.02 | 24.08 | 4.54 | -1.26 | 5.31 | 1.23 | 5.72 | 4.24 | -16.93 | 4.75 | 1.04 | 20.94 |
Endosome | -0.61 | 5.75 | -3.43 | -3.78 | -10.14 | -12.51 | 1.19 | -10.58 | -0.17 | -10.80 | -12.75 | 5.62 | -11.67 | -2.35 | -15.31 |
Golgi | -0.94 | 2.59 | -0.84 | -0.16 | -4.17 | 3.01 | 18.04 | 12.69 | 9.69 | -6.92 | 3.54 | 18.28 | 8.84 | 6.61 | -7.78 |
Lipid Particles | -10.44 | 5.08 | -7.75 | -0.57 | -10.88 | -0.82 | 5.53 | -1.80 | -1.16 | -6.34 | -3.68 | 12.42 | -4.00 | -1.12 | -14.18 |
Mitochondria | 0.98 | 1.97 | 1.74 | 3.68 | -2.20 | -2.76 | 7.19 | 5.17 | 6.28 | -3.20 | -1.26 | 7.00 | 5.14 | 7.05 | -4.91 |
Mitotic Spindle | 1.26 | 2.13 | -1.53 | -3.38 | -4.57 | -2.43 | -2.88 | -3.81 | -3.14 | -3.11 | -0.67 | 0.74 | -4.81 | -4.37 | -5.32 |
None | -3.09 | -3.65 | -1.60 | 2.35 | 1.47 | 2.54 | -0.06 | 2.01 | -0.21 | 1.73 | 1.76 | 1.25 | 2.25 | 0.77 | 1.25 |
Nuclear Periphery | -3.71 | -6.08 | 0.67 | 4.29 | 7.03 | -2.83 | -10.12 | -0.62 | 2.58 | 10.06 | -5.10 | -12.41 | -0.57 | 5.07 | 12.42 |
Nuclear Periphery Foci | -0.57 | -6.24 | -7.21 | -7.11 | -3.70 | -2.46 | -15.10 | -11.75 | -11.15 | 7.21 | -6.10 | -20.67 | -14.74 | -12.12 | 3.70 |
Nucleolus | -2.33 | 3.30 | -0.12 | 2.32 | -3.90 | -1.50 | -2.36 | 0.38 | 1.91 | 2.52 | -2.41 | 0.59 | 0.66 | 2.54 | -0.12 |
Nucleus | -1.03 | 0.44 | 3.31 | 7.60 | 5.64 | 1.91 | -8.91 | 0.70 | -0.86 | 9.01 | -1.26 | -7.62 | 2.54 | 4.09 | 9.68 |
Peroxisomes | -3.93 | 3.94 | 0.27 | 4.61 | -4.88 | 0.00 | 7.30 | 5.97 | 5.08 | -2.62 | -0.53 | 8.68 | 5.90 | 6.65 | -6.23 |
Vacuole | 18.55 | 22.91 | 10.77 | -10.19 | -17.38 | -13.93 | -5.79 | -18.07 | -6.79 | -12.69 | 0.52 | 12.02 | -9.26 | -11.23 | -22.64 |
Vacuole Periphery | -0.78 | 3.39 | -0.73 | -0.14 | -4.70 | -7.15 | 7.04 | -1.68 | 4.12 | -3.98 | -7.71 | 8.09 | -2.43 | 3.50 | -6.14 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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