Standard name
Human Ortholog
Description Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen; ARE2 has a paralog, ARE1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0.05 0 0.05 0.05
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.05 0.05 0 – 0 0 0 0 0 0 0 0 0.12 0.1 0.17 0 0 0 0 0.06 0.07
Cytoplasm 0.7 0.89 0.7 0.57 0.58 0.61 – 0.63 0.61 0.59 0.55 0.58 0.86 0.87 0.94 0.76 0.71 0.65 0.28 0.46 0.07 0.21 0.32 0.31
Endoplasmic Reticulum 0.27 0.05 0.17 0.26 0.18 0.06 – 0 0 0 0 0 0.13 0.13 0.05 0.09 0.05 0.05 0.52 0.22 0.65 0.48 0.32 0.3
Endosome 0 0 0 0 0.11 0.05 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.1 0.08 0.17 0.09 0.33 – 0.34 0.44 0.5 0.52 0.42 0 0 0 0.12 0.14 0.11 0.11 0.17 0.13 0.17 0.15 0.13
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.07 0 0.13 0.12 – 0.06 0 0 0 0.06 0 0 0 0 0.1 0.17 0 0.05 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 4 4 1 0 – 0 2 0 1 0 2 2 2 2 1 1 4 13 25 15 39 37
Bud 0 8 0 3 0 9 – 13 13 13 20 17 0 0 0 1 0 0 2 2 6 2 12 16
Bud Neck 0 0 0 0 0 0 – 1 0 0 0 0 6 6 6 0 0 0 2 3 4 6 12 20
Bud Site 0 0 0 0 0 0 – 1 0 5 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 4 12 14 16 12 – 4 4 5 2 6 13 13 6 4 2 11 3 4 10 9 42 52
Cytoplasm 115 239 268 164 193 205 – 426 379 446 295 386 402 402 342 25 15 42 57 153 35 85 238 240
Endoplasmic Reticulum 44 14 66 74 60 19 – 10 20 13 9 4 63 60 18 3 1 3 107 74 315 199 244 236
Endosome 2 0 7 7 35 18 – 36 19 16 16 28 1 1 0 0 0 0 1 1 4 1 3 9
Golgi 0 0 1 0 1 0 – 1 0 0 0 0 0 0 0 0 0 1 1 0 2 0 2 1
Mitochondria 4 26 31 50 30 110 – 231 274 377 278 279 0 0 0 4 3 7 21 56 62 72 114 104
Nucleus 0 0 1 1 0 0 – 2 1 1 0 1 2 2 1 0 0 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 1 0 – 1 0 1 1 1 1 0 0 0 0 0 0 0 3 0 0 1
Nucleolus 0 0 1 0 0 0 – 2 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 1 1 3 0 – 2 0 1 3 9 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 1 2 1 0 – 10 4 6 8 13 0 0 0 1 0 0 0 0 2 0 3 4
Vac/Vac Membrane 3 2 26 7 44 40 – 42 27 28 23 43 2 2 1 0 2 11 2 16 5 16 27 40
Unique Cell Count 165 269 383 287 331 335 679 623 754 534 666 467 463 365 33 21 65 207 334 488 416 753 780
Labelled Cell Count 170 295 419 327 385 413 782 743 912 657 789 492 488 376 40 24 77 207 334 488 416 753 780


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 4.0 5.4 6.0 6.1 4.8 – 4.1 3.8 3.4 3.6 3.6 6.4 6.4 6.2 5.1 6.7 7.5 6.3 6.3 6.4
Std Deviation (1e-4) 0.7 0.6 1.7 2.2 1.8 1.5 – 1.3 1.3 0.9 1.0 1.1 0.9 0.9 0.9 1.1 2.6 1.5 1.2 1.4 1.6
Intensity Change (Log2) – – – 0.15 0.17 -0.17 – -0.41 -0.5 -0.67 -0.59 -0.59 0.25 0.25 0.2 -0.08 0.33 0.48 0.22 0.23 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – 0
Bud Neck – – – – – – – – – – – – – – 0
Bud Site – – – – – – – – – – – – – – 0
Cell Periphery – – – – – – – – – – – – – – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – 0
Endosome – – – – – – – – – – – – – – 0
Golgi – – – – – – – – – – – – – – 0
Mitochondria – – – – – – – – – – – – – – 0
Nuclear Periphery – – – – – – – – – – – – – – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – 0
Peroxisomes – – – – – – – – – – – – – – 0
SpindlePole – – – – – – – – – – – – – – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – 0
Cytoplasm – – – – – – – – – – – – – – 0
Nucleus – – – – – – – – – – – – – – 0
Vacuole – – – – – – – – – – – – – – 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6553 2.2269 2.3816 2.0361 1.3385 2.1716 2.0817 2.8761 2.1785 2.386 1.8955 2.4839 1.7123 2.982 2.3171 2.4506 2.2171 2.4249
Actin 0.0103 0.001 0.0107 0.0003 0.0009 0.0048 0.0309 0.0005 0.0183 0.0211 0.0076 0.001 0.0142 0.0053 0.0072 0.0035 0.003 0.0019
Bud 0.0001 0.0001 0.0002 0.0001 0.0001 0.0002 0.0002 0.0021 0.0004 0.0014 0.0003 0.0001 0.0038 0.01 0.0035 0.0061 0.0437 0.0018
Bud Neck 0.0005 0.0003 0.0016 0.0003 0.0002 0.0027 0.0016 0.0003 0.0012 0.0119 0.0007 0.0038 0.0006 0.0015 0.0018 0.0043 0.0008 0.0061
Bud Periphery 0.0001 0.0005 0.0004 0.0001 0.0006 0.0001 0.0003 0.0008 0.0017 0.0008 0.0012 0.0002 0.0011 0.002 0.0026 0.0032 0.0145 0.0012
Bud Site 0.0005 0.0033 0.0022 0.0007 0.0001 0.0001 0.0026 0.0263 0.011 0.0007 0.0004 0.0002 0.0014 0.0305 0.009 0.0013 0.0006 0.0003
Cell Periphery 0.0031 0.004 0.0023 0.0009 0.0016 0.001 0.0013 0.0047 0.0053 0.0041 0.0012 0.001 0.0037 0.0065 0.0042 0.0043 0.001 0.0011
Cytoplasm 0.243 0.4054 0.2766 0.2601 0.4113 0.3258 0.2447 0.3576 0.2803 0.2135 0.2379 0.3232 0.2169 0.3641 0.3319 0.2472 0.261 0.3178
Cytoplasmic Foci 0.0074 0.0106 0.013 0.023 0.0033 0.0079 0.013 0.0049 0.0243 0.0165 0.0132 0.0117 0.0206 0.009 0.0161 0.0095 0.0122 0.0142
Eisosomes 0.0003 0.0001 0.0002 0 0 0 0.0002 0.0001 0.0006 0.001 0.0002 0 0.0005 0.0002 0.0003 0.0002 0.0001 0
Endoplasmic Reticulum 0.5946 0.4565 0.5783 0.5967 0.5301 0.5685 0.5729 0.4722 0.4946 0.6228 0.5696 0.5789 0.5574 0.4537 0.5207 0.6118 0.5249 0.5535
Endosome 0.0145 0.0089 0.0187 0.0078 0.0067 0.0114 0.0172 0.0073 0.0237 0.0188 0.0195 0.0095 0.0137 0.0069 0.0077 0.0103 0.0216 0.0129
Golgi 0.0025 0.0015 0.0032 0.0005 0.0005 0.0014 0.0041 0.001 0.0058 0.0021 0.007 0.0008 0.0038 0.0018 0.0016 0.0026 0.0017 0.0009
Lipid Particles 0.016 0.0096 0.0114 0.008 0.0028 0.005 0.0102 0.0045 0.0218 0.0087 0.0078 0.0039 0.0267 0.0077 0.0082 0.0069 0.0127 0.0102
Mitochondria 0.0004 0.0006 0.0008 0.0008 0.0001 0.0059 0.0028 0.0004 0.0032 0.0009 0.0342 0.0003 0.0018 0.0012 0.0011 0.0011 0.0023 0.0005
None 0.0838 0.0692 0.0612 0.0826 0.0332 0.0485 0.0772 0.1057 0.0835 0.0505 0.0413 0.0535 0.104 0.0655 0.063 0.0645 0.0809 0.0556
Nuclear Periphery 0.0112 0.0077 0.0087 0.006 0.0048 0.0082 0.0096 0.0046 0.0081 0.0119 0.0139 0.0052 0.0107 0.0109 0.0075 0.009 0.0095 0.007
Nucleolus 0.0004 0.0002 0.0001 0.0002 0 0.0001 0.0004 0.0004 0.0002 0.0006 0.0001 0 0.0011 0.0009 0.0006 0.0003 0.0002 0.0003
Nucleus 0.0023 0.0125 0.0017 0.0017 0.001 0.0029 0.0012 0.0016 0.0017 0.0026 0.0022 0.0015 0.0053 0.0118 0.0025 0.0043 0.0041 0.006
Peroxisomes 0.0003 0.0001 0.0002 0.0001 0 0.0006 0.0022 0 0.0066 0.0002 0.0334 0.0003 0.0021 0.0001 0.0009 0.0003 0.0003 0.0001
Punctate Nuclear 0.0041 0.0011 0.0016 0.0054 0.0003 0.0016 0.0038 0.0005 0.0014 0.0052 0.0006 0.0005 0.0056 0.0059 0.0042 0.0035 0.0015 0.0046
Vacuole 0.0044 0.0064 0.0064 0.0043 0.0021 0.003 0.0033 0.0041 0.0058 0.0043 0.0053 0.004 0.0045 0.0041 0.005 0.0053 0.003 0.0036
Vacuole Periphery 0.0004 0.0004 0.0005 0.0003 0.0001 0.0004 0.0005 0.0003 0.0006 0.0006 0.0023 0.0003 0.0005 0.0003 0.0003 0.0005 0.0005 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.3104 15.8516 23.1456 27.0825 18.17 29.728 33.8646 40.8273 38.0693 28.8295
Translational Efficiency 1.3287 1.86 1.1126 1.1031 1.3221 1.4428 1.138 0.8572 1.137 1.1727

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
150 1040 1556 848 1574 147 1221 189 1724 1187 2777 1037

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1002.81 750.77 960.38 1260.20 650.97 796.96 1102.85 1443.72 681.58 756.49 1023.02 1293.65
Standard Deviation 136.76 107.28 129.17 240.56 104.51 97.07 150.40 307.27 146.40 107.16 155.87 263.73
Intensity Change Log 2 — -0.417605 -0.062371 0.329604 — 0.291916 0.760574 1.149128 — -0.095614 0.319137 0.709285

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002878 0.000452 0.001155 0.012881 0.000254 0.000650 0.003465 0.012650 0.000482 0.000476 0.002170 0.012839
Bud Neck 0.010854 0.009532 0.006821 0.008103 0.008825 0.013600 0.005323 0.012260 0.009001 0.010036 0.006162 0.008861
Bud Site 0.017744 0.009425 0.010456 0.053808 0.003779 0.006000 0.025443 0.047378 0.004994 0.009001 0.017046 0.052636
Cell Periphery 0.002040 0.000362 0.001340 0.023592 0.000348 0.001251 0.006226 0.032700 0.000495 0.000472 0.003488 0.025252
Cytoplasm 0.181176* 0.426508* 0.343368* 0.083999 0.396816* 0.268315* 0.080063 0.051879 0.378053* 0.406918* 0.227597* 0.078145
Cytoplasmic Foci 0.097310 0.224773* 0.048456 0.018496 0.221302* 0.298360* 0.014075 0.013454 0.210513* 0.233886* 0.033339 0.017577
Eisosomes 0.000671 0.000373 0.000306 0.000905 0.000525 0.001074 0.000547 0.001011 0.000538 0.000459 0.000412 0.000925
Endoplasmic Reticulum 0.079213 0.046873 0.223608* 0.617607* 0.040205 0.066569 0.776218* 0.580540* 0.043599 0.049312 0.466581* 0.610851*
Endosome 0.028882 0.016612 0.009191 0.016275 0.014121 0.038397 0.012634 0.016792 0.015405 0.019310 0.010705 0.016370
Golgi 0.014823 0.008793 0.000798 0.006952 0.008672 0.015869 0.003532 0.013385 0.009207 0.009669 0.002000 0.008125
Lipid Particles 0.025015 0.033400 0.016771 0.071326 0.069215 0.090185 0.018234 0.142352* 0.065370 0.040433 0.017414 0.084271
Mitochondria 0.004586 0.004952 0.000921 0.001361 0.008492 0.016716 0.001882 0.001080 0.008152 0.006409 0.001344 0.001310
Mitotic Spindle 0.004318 0.002062 0.001093 0.009017 0.001168 0.001367 0.000808 0.005404 0.001442 0.001976 0.000968 0.008358
None 0.000191 0.008731 0.005038 0.001936 0.010172 0.000841 0.001619 0.000183 0.009304 0.007754 0.003535 0.001616
Nuclear Periphery 0.003651 0.002310 0.014766 0.001082 0.004683 0.006297 0.001711 0.000523 0.004593 0.002804 0.009026 0.000980
Nuclear Periphery Foci 0.024527 0.006385 0.036585 0.020155 0.003336 0.003789 0.010634 0.021036 0.005180 0.006064 0.025175 0.020315
Nucleolus 0.000468 0.002396 0.000819 0.000190 0.002014 0.001318 0.000503 0.000032 0.001880 0.002263 0.000680 0.000161
Nucleus 0.110035 0.120800 0.207468* 0.007875 0.155836* 0.065185 0.008647 0.002257 0.151851* 0.113912 0.120050 0.006851
Peroxisomes 0.003456 0.004300 0.001547 0.002347 0.004949 0.013601 0.001474 0.001674 0.004819 0.005452 0.001515 0.002224
Vacuole 0.382909* 0.069227 0.069156 0.041225 0.044004 0.083373 0.025577 0.041580 0.073491 0.070979 0.049995 0.041290
Vacuole Periphery 0.005254 0.001736 0.000337 0.000870 0.001284 0.007242 0.001384 0.001828 0.001629 0.002418 0.000797 0.001045

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.14 1.44 -4.89 -6.87 -6.45 -0.98 -5.42 -3.02 -2.91 -2.21 -0.06 -5.84 -7.46 -7.46 -6.37
Bud Neck 0.14 0.77 0.24 0.29 -1.78 -1.59 3.12 -1.70 0.11 -3.05 -0.85 2.61 -0.82 0.12 -3.58
Bud Site 1.89 1.60 -6.00 -9.02 -8.89 -1.30 -7.67 -5.16 -4.84 -2.49 -2.59 -7.85 -11.33 -10.07 -8.15
Cell Periphery 2.61 0.68 -8.56 -9.35 -8.86 -1.14 -8.95 -6.18 -5.93 -4.99 -0.03 -8.50 -11.04 -11.03 -9.52
Cytoplasm -14.56 -9.83 4.94 35.35 28.52 7.01 43.13 29.14 10.55 2.07 -3.25 19.77 38.18 36.87 20.66
Cytoplasmic Foci -14.18 4.65 8.33 33.48 9.85 -4.33 42.50 38.38 16.37 0.09 -3.43 38.05 40.61 37.10 6.80
Eisosomes 1.62 2.10 -1.56 -3.51 -3.93 -1.08 -0.32 -2.07 0.13 -1.87 0.84 2.06 -3.04 -3.14 -3.87
Endoplasmic Reticulum 1.18 -9.93 -28.05 -38.87* -24.28 -1.95 -76.64* -18.99 -16.32 7.22 -1.41 -49.89 -44.14 -42.37 -8.74
Endosome 1.73 3.48 1.64 -0.16 -5.52 -4.54 0.99 -1.15 3.76 -1.61 -3.40 4.32 -1.41 1.64 -4.80
Golgi 0.52 2.20 0.91 1.29 -5.00 -1.51 6.63 -0.87 0.43 -1.92 -0.60 9.86 0.67 1.03 -4.40
Lipid Particles -3.53 1.60 -9.13 -7.77 -11.33 -1.87 16.23 -4.46 -2.71 -7.54 7.88 18.59 -3.82 -8.41 -13.28
Mitochondria -0.56 1.02 0.85 5.21 -1.35 -1.31 6.07 6.35 2.53 1.30 1.23 6.84 6.78 5.21 0.23
Mitotic Spindle 0.47 0.78 -2.30 -3.81 -4.30 -0.19 0.57 -1.09 -1.03 -1.16 -0.67 0.63 -4.24 -3.89 -4.47
None -5.65 -4.92 -2.51 4.12 2.57 6.11 5.28 6.55 2.30 2.15 0.92 3.93 5.21 4.27 2.27
Nuclear Periphery 1.01 -7.38 2.42 3.04 9.75 -0.83 3.79 6.08 3.11 3.05 2.46 -4.34 5.34 4.44 9.73
Nuclear Periphery Foci 3.80 -3.47 -0.27 -6.63 4.58 -0.46 -6.88 -3.84 -3.69 -2.24 -1.32 -15.98 -8.29 -7.44 1.09
Nucleolus -2.88 -2.36 1.38 3.13 3.02 1.78 4.15 9.69 3.32 1.45 -0.63 4.71 8.03 3.39 2.66
Nucleus -1.36 -7.19 6.36 24.80 35.04 12.04 36.12 37.61 9.58 4.17 6.76 5.40 36.26 26.16 29.77
Peroxisomes -0.90 1.46 0.72 3.31 -1.65 -3.24 9.06 6.83 4.46 -0.43 -1.20 9.83 5.45 5.32 -1.81
Vacuole 14.34 14.08 14.95 3.72 4.99 -4.85 5.98 -0.46 3.54 -2.85 1.72 5.60 5.57 4.73 1.07
Vacuole Periphery 1.51 2.44 2.08 5.03 -4.41 -1.65 -0.28 -0.67 1.48 -0.47 -1.84 3.26 2.22 2.81 -1.03
Standard name
Human Ortholog
Description Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen; ARE2 has a paralog, ARE1, that arose from the whole genome duplication
Localization
Cell Percentages ER (33%), cytoplasm (4%), mixed (44%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Are2

Are2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Are2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available