Standard name
Human Ortholog
Description Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0.08 0.06 0.09 – 0 0 0.05 0 0.1 0.14 0.17 0 0.05 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0 0.44 0.07 – 0.31 0.22 0.3 0.29 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.96 0.94 0.96 0.91 0.89 0.86 0.86 – 0.84 0.85 0.84 0.88 0.88 0.76 0.74 0.86 0.89 0.88 0.88 0.92 0.91 0.78 0.59 0.53
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0.07 0.08 0.05 0 0.05 0 0 0 0.06 0.11 0.1
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0 0.05 0 0 0 0.12 0.21 0.27
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 0 3 0 – 2 1 1 2 0 0 0 0 0 0 2 2 0 0 2 2
Bud Neck 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 1 0 1 0 11 9 – 10 7 6 13 0 1 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 4 6 14 16 16 29 – 13 15 14 7 40 67 77 5 5 4 1 2 0 0 1 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 2 1 1 0 0 0 0 0 2
Endosome 0 0 0 1 0 0 1 – 1 2 0 1 0 2 7 0 0 0 3 0 1 2 6 9
Golgi 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 1 0 0 3 2 0 1 1 1
Mitochondria 9 18 0 8 1 125 24 – 95 74 81 85 2 1 0 1 1 1 2 2 4 1 8 8
Nucleus 364 337 233 208 182 247 287 – 260 291 223 253 357 357 345 124 85 88 350 347 222 205 278 243
Nuclear Periphery 3 3 0 6 2 0 6 – 7 6 1 5 4 7 14 1 1 1 1 0 0 0 6 11
Nucleolus 7 1 2 2 2 2 4 – 8 11 8 6 13 32 38 7 4 5 12 4 3 15 52 46
Peroxisomes 1 1 0 0 0 0 0 – 0 1 0 0 0 3 7 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 1 0 1 – 0 2 1 1 1 11 5 0 0 0 1 1 0 2 6 5
Vac/Vac Membrane 6 13 2 3 7 1 10 – 4 9 2 2 1 10 8 10 2 5 15 13 7 32 97 123
Unique Cell Count 381 358 242 228 205 287 334 310 344 267 289 408 468 465 144 95 100 396 378 244 264 470 463
Labelled Cell Count 395 378 244 243 212 405 371 401 419 337 375 419 491 501 151 99 105 396 378 244 264 470 463


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.7 6.7 6.9 6.5 7.0 4.2 5.6 – 4.8 5.1 4.9 4.9 7.4 6.8 7.3 9.8 9.8 8.9 6.3 6.8 7.1
Std Deviation (1e-4) 1.2 1.3 1.2 1.3 1.9 1.3 1.8 – 1.4 1.4 1.3 1.1 1.8 1.6 1.9 3.0 2.6 2.0 1.3 1.2 1.6
Intensity Change (Log2) – – – -0.09 0.02 -0.71 -0.3 – -0.53 -0.43 -0.49 -0.48 0.09 -0.01 0.09 0.5 0.51 0.37 -0.13 -0.02 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 3.3 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.0 2.6 1.8 3.1 – 1.1 1.2 1.6 0 3.5 4.9 5.5 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.3 – 0 7.7 9.3 9.2 0 0 0 0 0 0
Nucleus -2.3 -3.1 -4.0 -4.1 – -4.7 -4.5 -4.7 -3.6 -3.7 -6.7 -7.2 -3.7 -2.4 -2.9
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 1.9 0 0 1.9 3.6 4.0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 1.8 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0013 1.3051 1.3744 0.9617 1.3978 1.2733 2.3989 2.5227 2.4471 1.9887 1.9024 2.4979 5.1618 5.4721 5.2137 5.5347 5.6193 5.39
Actin 0.061 0 0.0032 0.0116 0.0313 0.0057 0.0031 0 0.0023 0 0.0056 0.0002 0.0088 0 0.0095 0.0223 0.0166 0
Bud 0.0017 0 0.0004 0.001 0.0014 0.0002 0.0003 0 0.0001 0 0 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0
Bud Neck 0.0054 0.0002 0.0004 0.0031 0.014 0.002 0.0031 0.0002 0.0004 0.0002 0.0005 0.0009 0.0016 0.0001 0.0004 0.0048 0.0006 0.0009
Bud Periphery 0.0041 0.0001 0.0023 0.0024 0.0019 0.0008 0.0006 0 0.0002 0 0.0001 0.0003 0.0005 0.0001 0.0004 0.0002 0.0001 0.0001
Bud Site 0.0159 0.0001 0.0009 0.004 0.0347 0.0006 0.0029 0.0001 0.0006 0 0 0.0001 0.0003 0.0002 0.0006 0.0011 0.0001 0.0001
Cell Periphery 0.0008 0.0001 0.0009 0.0007 0.0007 0.0003 0.0002 0 0.0001 0 0 0 0.0002 0 0.0001 0.0001 0 0
Cytoplasm 0.006 0.0001 0.0034 0.0012 0.0013 0.002 0.0007 0.0008 0.0004 0.0002 0.0001 0.0001 0.0021 0.0001 0.0004 0.0058 0.0007 0.0007
Cytoplasmic Foci 0.0255 0 0.0045 0.0035 0.0132 0.004 0.0033 0 0.0002 0 0.0001 0.0015 0.0006 0 0.0015 0.0083 0.0011 0.0007
Eisosomes 0.0003 0 0 0.0002 0.0005 0 0.0001 0 0 0 0.0001 0 0.0023 0 0.0001 0.0004 0.0001 0
Endoplasmic Reticulum 0.0044 0 0.0016 0.0022 0.0017 0.001 0.0016 0 0.0005 0 0.0008 0.0001 0.0039 0 0.0001 0.0016 0.0031 0.0004
Endosome 0.0219 0 0.004 0.0102 0.0419 0.0081 0.0048 0.0002 0.0008 0 0.0036 0.0003 0.0058 0 0.0011 0.0054 0.0181 0.0015
Golgi 0.0097 0 0.0041 0.0124 0.027 0.0027 0.001 0 0.0003 0 0.0009 0.0002 0.0012 0 0.0009 0.0027 0.0045 0.0001
Lipid Particles 0.0205 0 0.0118 0.0146 0.0596 0.0021 0.002 0 0.0003 0 0.0004 0.0016 0.0015 0 0.002 0.0135 0.0027 0.0007
Mitochondria 0.0164 0.0003 0.0121 0.0054 0.0201 0.0028 0.002 0.0001 0.0014 0.0001 0.0016 0.0015 0.0015 0.0004 0.007 0.001 0.0017 0.0001
None 0.0032 0 0.0004 0.0004 0.0007 0.0005 0.0009 0.0002 0.0001 0.0003 0.0003 0.0001 0.0137 0 0.0022 0.0444 0.0012 0.001
Nuclear Periphery 0.0265 0.0029 0.0125 0.0056 0.0073 0.0156 0.0113 0.0075 0.0061 0.0023 0.0159 0.0013 0.0162 0.0007 0.0014 0.0177 0.0163 0.0027
Nucleolus 0.0273 0.0495 0.0747 0.039 0.0129 0.062 0.04 0.0489 0.0668 0.0497 0.0298 0.0706 0.0392 0.0538 0.0663 0.055 0.0372 0.0601
Nucleus 0.709 0.9441 0.8531 0.8751 0.7091 0.8791 0.913 0.9379 0.9146 0.9454 0.937 0.911 0.8839 0.9437 0.8931 0.7796 0.8892 0.9142
Peroxisomes 0.0093 0 0.0033 0.0024 0.0095 0.0011 0.002 0 0.0002 0 0.0001 0.0021 0.0003 0 0.0043 0.0117 0.0003 0
Punctate Nuclear 0.0145 0.0019 0.0016 0.0019 0.0078 0.0065 0.0055 0.0032 0.0041 0.0016 0.0017 0.0075 0.0108 0.0003 0.0071 0.0227 0.0049 0.0163
Vacuole 0.0112 0.0004 0.0033 0.0024 0.0019 0.0019 0.001 0.0003 0.0003 0 0.0003 0.0003 0.004 0.0003 0.0008 0.001 0.0004 0.0004
Vacuole Periphery 0.0054 0.0002 0.0017 0.0008 0.0016 0.001 0.0007 0.0002 0.0002 0 0.0008 0.0003 0.0014 0.0002 0.0005 0.0005 0.0009 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.8508 12.3077 14.3516 9.4537 10.2004 11.7946 6.6666 8.3991 14.4054 12.339
Translational Efficiency 4.8763 2.7942 1.981 3.8011 3.5724 2.6882 3.5235 2.712 1.7443 2.5463

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1236 405 497 20 470 1019 2082 1254 1706 1424 2579 1274

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 707.46 874.05 1020.68 1066.13 779.00 926.50 1004.60 1018.72 727.17 911.58 1007.70 1019.46
Standard Deviation 124.05 176.89 133.72 122.57 118.92 133.09 131.99 162.30 126.76 148.78 132.47 161.86
Intensity Change Log 2 — 0.305067 0.528810 0.591663 — 0.250168 0.366926 0.387062 — 0.276557 0.446241 0.488063

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000223 0.000984 0.000848 0.001249 0.000403 0.000324 0.000493 0.000926 0.000273 0.000512 0.000562 0.000931
Bud Neck 0.002384 0.021969 0.017622 0.008635 0.002980 0.010878 0.030115 0.024843 0.002548 0.014032 0.027708 0.024588
Bud Site 0.000465 0.002212 0.001177 0.001422 0.000329 0.001639 0.001071 0.003505 0.000427 0.001802 0.001091 0.003472
Cell Periphery 0.000171 0.000358 0.000044 0.000062 0.000074 0.000195 0.000065 0.000118 0.000144 0.000241 0.000061 0.000117
Cytoplasm 0.007685 0.006416 0.000497 0.001537 0.002036 0.004119 0.001346 0.004447 0.006129 0.004772 0.001182 0.004402
Cytoplasmic Foci 0.000928 0.000808 0.000023 0.000024 0.000381 0.001075 0.000105 0.001959 0.000777 0.000999 0.000089 0.001929
Eisosomes 0.000016 0.000032 0.000032 0.000046 0.000023 0.000029 0.000028 0.000027 0.000018 0.000030 0.000029 0.000027
Endoplasmic Reticulum 0.004700 0.008722 0.004798 0.005947 0.004882 0.012467 0.004884 0.005834 0.004750 0.011402 0.004868 0.005836
Endosome 0.000410 0.001222 0.000147 0.000361 0.000244 0.001242 0.000644 0.001604 0.000364 0.001236 0.000548 0.001584
Golgi 0.000333 0.000416 0.000051 0.000109 0.000061 0.000546 0.000140 0.002135 0.000258 0.000509 0.000123 0.002103
Lipid Particles 0.000235 0.000187 0.000307 0.000185 0.000343 0.000158 0.000061 0.000269 0.000265 0.000166 0.000108 0.000268
Mitochondria 0.000889 0.001794 0.001151 0.001251 0.002375 0.002249 0.001500 0.002769 0.001298 0.002120 0.001433 0.002745
Mitotic Spindle 0.000532 0.002731 0.000149 0.000663 0.000244 0.002268 0.003409 0.013460 0.000453 0.002400 0.002781 0.013259
None 0.013689 0.011106 0.000790 0.002292 0.004170 0.003796 0.001727 0.003753 0.011066 0.005875 0.001547 0.003730
Nuclear Periphery 0.000517 0.001019 0.000313 0.001260 0.000519 0.001284 0.000901 0.002285 0.000517 0.001208 0.000788 0.002269
Nuclear Periphery Foci 0.000307 0.000573 0.000068 0.000234 0.000422 0.000204 0.000174 0.001339 0.000339 0.000309 0.000154 0.001322
Nucleolus 0.016624 0.034100 0.008036 0.046377 0.031121 0.026417 0.014544 0.031432 0.020618 0.028602 0.013290 0.031667
Nucleus 0.948430* 0.901123* 0.961991* 0.924919* 0.948198* 0.921349* 0.936561* 0.892737* 0.948366* 0.915596* 0.941462* 0.893242*
Peroxisomes 0.000609 0.000580 0.000556 0.000528 0.000474 0.000357 0.000114 0.000279 0.000572 0.000421 0.000199 0.000283
Vacuole 0.000584 0.003049 0.001282 0.002622 0.000535 0.006894 0.001743 0.003980 0.000571 0.005800 0.001654 0.003959
Vacuole Periphery 0.000268 0.000599 0.000117 0.000279 0.000187 0.002512 0.000375 0.002301 0.000246 0.001968 0.000326 0.002269

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -11.21 -5.45 -3.73 -1.40 -1.63 1.26 -3.27 -6.51 -8.22 -5.15 -6.60 -10.58 -9.48 -6.20 -4.51
Bud Neck -8.19 -10.17 -2.33 1.60 1.10 -7.94 -24.63 -18.20 -11.60 3.76 -11.95 -27.49 -19.27 -9.10 2.35
Bud Site -5.79 -3.69 -2.25 0.23 -1.06 -2.73 -10.00 -5.33 -2.59 -3.46 -3.99 -9.58 -5.21 -2.53 -3.52
Cell Periphery -1.39 6.48 3.61 2.18 -0.95 -4.04 0.74 -1.70 2.94 -3.11 -2.17 5.79 1.96 3.06 -3.40
Cytoplasm 0.73 6.06 4.21 2.63 -1.11 -1.57 0.59 -2.64 -0.44 -3.04 1.01 5.26 0.97 -0.06 -3.29
Cytoplasmic Foci 0.47 3.00 2.95 1.91 -0.76 -1.27 2.13 -1.64 -0.47 -2.21 -0.27 3.15 -0.83 -0.60 -2.23
Eisosomes -6.65 -6.64 -2.73 -1.62 -1.60 -2.18 -2.95 -2.93 -0.13 -0.37 -6.31 -9.17 -7.25 0.20 -0.07
Endoplasmic Reticulum -6.55 -0.86 -1.45 1.28 -1.23 -7.61 -0.45 -1.95 6.82 -2.16 -9.45 -1.31 -3.04 7.54 -2.33
Endosome -2.34 3.51 -0.68 1.69 -2.08 -3.95 -1.99 -3.87 -0.33 -2.39 -4.10 -0.82 -3.38 -0.32 -2.75
Golgi -0.18 1.50 0.76 1.01 -2.32 -1.71 -1.78 -2.35 -1.49 -2.23 -0.87 1.05 -1.96 -1.59 -2.26
Lipid Particles 1.02 -0.20 -0.25 -0.61 0.04 2.07 2.99 0.56 -1.22 -2.06 2.49 2.71 -0.03 -1.13 -1.53
Mitochondria -2.69 -1.09 -1.18 0.94 -0.49 0.06 0.36 -0.23 -0.82 -2.45 -1.34 -0.66 -2.26 -1.17 -2.74
Mitotic Spindle -1.00 1.18 -1.18 -0.11 -1.37 -2.07 -3.88 -5.70 -4.74 -4.04 -2.04 -3.53 -5.63 -4.72 -4.38
None 0.88 7.74 6.29 2.88 -1.69 0.31 2.25 0.50 0.22 -3.00 3.40 7.51 5.62 2.04 -3.47
Nuclear Periphery -3.13 2.19 -1.14 -0.60 -1.29 -4.91 -4.15 -4.43 -2.56 -3.00 -5.86 -4.02 -4.50 -2.76 -3.38
Nuclear Periphery Foci -1.71 3.30 -0.97 -0.11 -1.84 1.16 1.36 -2.46 -3.24 -3.35 0.43 2.39 -2.86 -2.98 -3.43
Nucleolus -4.78 4.11 -1.89 -1.52 -2.05 1.33 4.14 -1.51 -3.17 -6.56 -3.41 2.89 -5.80 -3.05 -7.50
Nucleus 6.16 -2.04 1.79 0.87 1.96 4.08 4.87 9.77 5.95 7.18 6.79 5.19 11.20 5.53 8.54
Peroxisomes 0.56 0.53 0.34 -0.38 -0.33 0.87 4.24 2.10 0.48 -5.52 0.96 1.66 1.36 1.21 -1.49
Vacuole -2.53 -3.37 -1.40 -0.44 -1.09 -4.64 -4.54 -5.48 0.74 -3.13 -5.17 -4.91 -5.50 0.20 -3.43
Vacuole Periphery -1.86 1.13 -0.92 1.02 -2.59 -4.11 -1.99 -2.87 0.87 -2.57 -4.13 -0.52 -2.74 0.26 -2.66
Standard name
Human Ortholog
Description Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Mpp6

Mpp6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mpp6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available