Standard name | |
---|---|
Human Ortholog | |
Description | Guanine nucleotide exchange factor (GEF); activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER; SEC12 has a paralog, SED4, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 12 | 14 | 14 | 2 | 2 | 2 | 6 | 5 | 1 | 2 | 3 | 11 | 11 | 8 | – | 1 | 4 | 3 | 7 | 15 | 12 | 19 | 14 |
Bud | 0 | 2 | 0 | 4 | 0 | 10 | 5 | 12 | 9 | 13 | 15 | 15 | 2 | 2 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 1 | 0 | 3 | 4 | 5 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 8 | 4 | 27 | 18 | 12 | 24 | 21 | 30 | 13 | 23 | 21 | 29 | 21 | 21 | 27 | – | 2 | 2 | 1 | 2 | 10 | 10 | 19 | 24 |
Cytoplasm | 25 | 65 | 78 | 51 | 83 | 107 | 141 | 159 | 102 | 130 | 111 | 119 | 141 | 141 | 142 | – | 13 | 7 | 3 | 15 | 1 | 12 | 23 | 17 |
Endoplasmic Reticulum | 140 | 156 | 278 | 139 | 70 | 103 | 100 | 136 | 87 | 58 | 47 | 54 | 66 | 65 | 123 | – | 0 | 0 | 181 | 198 | 368 | 268 | 462 | 473 |
Endosome | 0 | 0 | 3 | 7 | 16 | 10 | 10 | 15 | 3 | 3 | 2 | 9 | 1 | 1 | 0 | – | 1 | 1 | 0 | 2 | 1 | 2 | 2 | 3 |
Golgi | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 0 | 1 | 2 | 1 | 2 | 2 |
Mitochondria | 6 | 9 | 39 | 100 | 56 | 136 | 149 | 222 | 174 | 225 | 237 | 185 | 2 | 2 | 0 | – | 0 | 0 | 4 | 6 | 10 | 34 | 30 | 23 |
Nucleus | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 4 | 0 | 1 | 1 | 1 | 2 | 2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 5 | 1 | 1 | 1 | 7 | 10 | 9 | 8 | 2 | 10 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 1 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 7 | 9 | 16 | 24 | 75 | 52 | 99 | 87 | 28 | 61 | 32 | 55 | 45 | 50 | 15 | – | 1 | 1 | 4 | 7 | 1 | 12 | 16 | 20 |
Unique Cell Count | 170 | 222 | 383 | 277 | 262 | 339 | 424 | 532 | 336 | 404 | 381 | 376 | 260 | 265 | 282 | 18 | 16 | 204 | 250 | 422 | 362 | 587 | 593 | |
Labelled Cell Count | 192 | 259 | 461 | 361 | 316 | 445 | 537 | 683 | 431 | 527 | 472 | 484 | 292 | 296 | 315 | 18 | 17 | 204 | 250 | 422 | 362 | 587 | 593 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.0 | 7.5 | 6.9 | 7.0 | 6.9 | 5.8 | 5.7 | 5.6 | 5.0 | 5.2 | 4.9 | 5.5 | 7.8 | 7.8 | 7.8 | – | 9.9 | 10.5 | 8.1 | 8.5 | 8.8 |
Std Deviation (1e-4) | 0.8 | 1.4 | 1.3 | 1.3 | 1.4 | 1.5 | 1.4 | 1.1 | 1.2 | 1.1 | 1.1 | 0.9 | 1.0 | 1.0 | 0.9 | – | 2.6 | 2.4 | 1.2 | 1.4 | 1.3 |
Intensity Change (Log2) | – | – | – | 0.01 | -0.0 | -0.26 | -0.28 | -0.32 | -0.46 | -0.41 | -0.5 | -0.33 | 0.16 | 0.16 | 0.16 | – | 0.52 | 0.6 | 0.22 | 0.3 | 0.35 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.9 | -2.3 | -2.8 | -3.2 | -2.6 | -1.8 | -3.5 | -3.0 | -2.7 | 0.4 | 0.3 | -0.6 | – | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 3.0 | 0 | 3.5 | 3.9 | 3.9 | 0 | 0 | 0 | – | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cell Periphery | -0.3 | -1.3 | 0 | -1.3 | -0.9 | -1.9 | -0.8 | -0.9 | 0.3 | 0.5 | 0.4 | 1.2 | – | 0 | 0 |
Cytoplasm | -0.6 | 3.3 | 3.4 | 4.1 | 3.2 | 3.1 | 3.8 | 2.8 | 3.5 | 8.9 | 8.7 | 8.1 | – | 0 | 0 |
Endoplasmic Reticulum | -5.9 | -11.5 | -11.3 | -13.9 | -14.1 | -12.5 | -16.5 | -16.8 | -16.2 | -11.8 | -12.0 | -7.5 | – | -6.5 | -6.2 |
Endosome | 0 | 3.9 | 2.2 | 1.8 | 2.2 | 0 | 0 | 0 | 1.8 | 0 | 0 | 0 | – | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Mitochondria | 8.1 | 3.9 | 9.4 | 8.4 | 10.4 | 12.2 | 13.5 | 15.0 | 11.8 | -4.8 | -4.8 | -5.5 | – | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0.4 | 0.7 | 1.3 | 0.7 | 0 | 1.3 | 0 | 0 | 0 | – | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Vacuole | 2.4 | 8.8 | 5.1 | 7.8 | 5.7 | 2.3 | 5.2 | 2.4 | 4.9 | 5.6 | 6.1 | 0.7 | – | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.4842 | 4.9107 | 4.7924 | 4.184 | 4.3659 | 5.5713 | 5.484 | 6.4121 | 5.9177 | 5.4092 | 5.0585 | 5.7204 | 6.7559 | 7.0964 | 6.6965 | 6.2452 | 5.923 | 6.52 |
Actin | 0.014 | 0.0024 | 0.0053 | 0.0091 | 0.0044 | 0.0029 | 0.0316 | 0.0022 | 0.0016 | 0.0001 | 0.0018 | 0.0032 | 0.0057 | 0.002 | 0.0015 | 0.0006 | 0.0001 | 0.0012 |
Bud | 0.0003 | 0.0016 | 0.0003 | 0.0171 | 0.0005 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0 |
Bud Neck | 0.001 | 0.0007 | 0.0017 | 0.0013 | 0.0006 | 0.0025 | 0.0008 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0006 | 0.0003 | 0.0005 | 0.0008 | 0.0005 | 0.0002 | 0.0016 |
Bud Periphery | 0.0015 | 0.0007 | 0.0009 | 0.017 | 0.0009 | 0.0002 | 0.0003 | 0.0001 | 0.0005 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0 | 0 |
Bud Site | 0.0016 | 0.0044 | 0.009 | 0.0191 | 0.0062 | 0.0001 | 0.005 | 0.0025 | 0.001 | 0 | 0.0007 | 0.0001 | 0.0003 | 0.006 | 0.0007 | 0 | 0 | 0.0001 |
Cell Periphery | 0.0049 | 0.0081 | 0.0096 | 0.0017 | 0.001 | 0.0029 | 0.0026 | 0.0016 | 0.0025 | 0.0014 | 0.0017 | 0.0011 | 0.0025 | 0.0059 | 0.0021 | 0.0018 | 0.0009 | 0.0011 |
Cytoplasm | 0.1341 | 0.2506 | 0.212 | 0.1791 | 0.1295 | 0.2355 | 0.0429 | 0.1083 | 0.0876 | 0.0315 | 0.0653 | 0.0724 | 0.0908 | 0.0711 | 0.0625 | 0.0628 | 0.0275 | 0.1065 |
Cytoplasmic Foci | 0.0166 | 0.0099 | 0.0206 | 0.0127 | 0.0086 | 0.0038 | 0.0097 | 0.0029 | 0.0075 | 0.0024 | 0.0121 | 0.005 | 0.0114 | 0.0026 | 0.0052 | 0.0009 | 0.0006 | 0.0044 |
Eisosomes | 0.0008 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0 | 0 | 0.0004 | 0.0006 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.6243 | 0.5944 | 0.6241 | 0.6535 | 0.726 | 0.6755 | 0.8035 | 0.8531 | 0.8654 | 0.9061 | 0.8296 | 0.8762 | 0.8182 | 0.8855 | 0.8738 | 0.9099 | 0.9617 | 0.8616 |
Endosome | 0.026 | 0.016 | 0.0219 | 0.0186 | 0.0096 | 0.0166 | 0.0214 | 0.01 | 0.0083 | 0.0092 | 0.0193 | 0.0086 | 0.0129 | 0.0055 | 0.0172 | 0.0037 | 0.0012 | 0.006 |
Golgi | 0.0094 | 0.0021 | 0.0054 | 0.0058 | 0.0019 | 0.0052 | 0.0139 | 0.0016 | 0.0012 | 0.0009 | 0.0117 | 0.0048 | 0.0034 | 0.0016 | 0.0028 | 0.0007 | 0.0003 | 0.0011 |
Lipid Particles | 0.0279 | 0.0123 | 0.0157 | 0.0036 | 0.0088 | 0.0037 | 0.0155 | 0.0036 | 0.0072 | 0.0165 | 0.0165 | 0.012 | 0.0116 | 0.01 | 0.0187 | 0.0052 | 0.0036 | 0.0038 |
Mitochondria | 0.0056 | 0.0009 | 0.0046 | 0.0127 | 0.0022 | 0.0023 | 0.0048 | 0.0004 | 0.0004 | 0.0001 | 0.0069 | 0.0012 | 0.001 | 0.0002 | 0.0006 | 0.0002 | 0 | 0.0001 |
None | 0.0642 | 0.0461 | 0.0107 | 0.0099 | 0.0029 | 0.0242 | 0.0212 | 0.0026 | 0.0054 | 0.0006 | 0.0232 | 0.003 | 0.0303 | 0.0006 | 0.0009 | 0.0047 | 0.0002 | 0.0064 |
Nuclear Periphery | 0.0374 | 0.0161 | 0.0082 | 0.011 | 0.0159 | 0.008 | 0.0071 | 0.0055 | 0.004 | 0.026 | 0.0037 | 0.0036 | 0.0041 | 0.0042 | 0.0063 | 0.0062 | 0.0024 | 0.0032 |
Nucleolus | 0.0006 | 0.0154 | 0.0209 | 0.0007 | 0.0066 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 |
Nucleus | 0.012 | 0.0089 | 0.0141 | 0.0065 | 0.0479 | 0.0016 | 0.0012 | 0.001 | 0.0006 | 0.0004 | 0.0005 | 0.0006 | 0.0004 | 0.0005 | 0.0011 | 0.0004 | 0.0002 | 0.0005 |
Peroxisomes | 0.0049 | 0.0006 | 0.0046 | 0.0008 | 0.0176 | 0.0003 | 0.0095 | 0.0001 | 0.0025 | 0.0001 | 0.002 | 0.004 | 0.0021 | 0 | 0.0002 | 0 | 0 | 0.0001 |
Punctate Nuclear | 0.0051 | 0.0021 | 0.004 | 0.0106 | 0.0047 | 0.0003 | 0.0043 | 0.0002 | 0.0002 | 0.0021 | 0.0016 | 0.0002 | 0.002 | 0.0002 | 0.0009 | 0.0001 | 0 | 0.0002 |
Vacuole | 0.0057 | 0.006 | 0.0056 | 0.007 | 0.0035 | 0.0102 | 0.0031 | 0.0036 | 0.0033 | 0.002 | 0.0017 | 0.0023 | 0.0021 | 0.0025 | 0.0036 | 0.0019 | 0.001 | 0.0019 |
Vacuole Periphery | 0.0019 | 0.0006 | 0.0008 | 0.0023 | 0.0005 | 0.0042 | 0.0006 | 0.0003 | 0.0002 | 0.0005 | 0.0006 | 0.0003 | 0.0003 | 0.0001 | 0.0006 | 0.0003 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 21.8187 | 18.808 | 20.1592 | 22.0175 | 17.1754 | 16.355 | 18.6646 | 20.9164 | 20.8438 | 26.6798 |
Translational Efficiency | 2.1175 | 1.4837 | 1.3366 | 1.1948 | 1.8674 | 2.7758 | 1.6556 | 1.27 | 1.5338 | 1.6304 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
185 | 1211 | 220 | 275 | 1706 | 1955 | 2031 | 1898 | 1891 | 3166 | 2251 | 2173 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1048.10 | 954.52 | 1229.16 | 1233.97 | 803.78 | 932.09 | 1159.43 | 1119.66 | 827.68 | 940.67 | 1166.25 | 1134.13 |
Standard Deviation | 125.70 | 162.20 | 135.33 | 165.41 | 124.78 | 124.42 | 150.27 | 156.62 | 144.44 | 140.50 | 150.31 | 162.28 |
Intensity Change Log 2 | — | -0.134929 | 0.229896 | 0.235531 | — | 0.213669 | 0.528543 | 0.478188 | — | 0.026806 | 0.367169 | 0.345897 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001310 | 0.001354 | 0.003745 | 0.006936 | 0.000842 | 0.000973 | 0.001776 | 0.003020 | 0.000888 | 0.001119 | 0.001968 | 0.003516 |
Bud Neck | 0.001504 | 0.001413 | 0.004059 | 0.004813 | 0.001915 | 0.002144 | 0.001198 | 0.002706 | 0.001875 | 0.001864 | 0.001477 | 0.002973 |
Bud Site | 0.007720 | 0.005256 | 0.012624 | 0.017087 | 0.007555 | 0.004402 | 0.005100 | 0.013942 | 0.007571 | 0.004729 | 0.005835 | 0.014340 |
Cell Periphery | 0.005584 | 0.002571 | 0.003744 | 0.010327 | 0.006004 | 0.001609 | 0.002444 | 0.005493 | 0.005963 | 0.001977 | 0.002571 | 0.006105 |
Cytoplasm | 0.059090 | 0.130657* | 0.053328 | 0.053362 | 0.141843* | 0.154220* | 0.051411 | 0.073831 | 0.133747* | 0.145207* | 0.051599 | 0.071241 |
Cytoplasmic Foci | 0.015663 | 0.033893 | 0.018183 | 0.009695 | 0.043623 | 0.027633 | 0.001883 | 0.006407 | 0.040888 | 0.030027 | 0.003476 | 0.006823 |
Eisosomes | 0.000945 | 0.000765 | 0.000246 | 0.000909 | 0.001441 | 0.000890 | 0.000238 | 0.000518 | 0.001393 | 0.000842 | 0.000239 | 0.000567 |
Endoplasmic Reticulum | 0.773214* | 0.718912* | 0.686531* | 0.760237* | 0.664223* | 0.693094* | 0.904163* | 0.844469* | 0.674886* | 0.702969* | 0.882893* | 0.833809* |
Endosome | 0.010446 | 0.007709 | 0.018240 | 0.009798 | 0.007443 | 0.006945 | 0.002765 | 0.006036 | 0.007737 | 0.007237 | 0.004278 | 0.006512 |
Golgi | 0.003916 | 0.018439 | 0.001517 | 0.004488 | 0.024508 | 0.019389 | 0.001046 | 0.004367 | 0.022494 | 0.019026 | 0.001092 | 0.004382 |
Lipid Particles | 0.018640 | 0.035523 | 0.022398 | 0.049486 | 0.040628 | 0.040082 | 0.004629 | 0.018779 | 0.038477 | 0.038338 | 0.006365 | 0.022665 |
Mitochondria | 0.000236 | 0.003891 | 0.000100 | 0.000444 | 0.017317 | 0.007792 | 0.000505 | 0.001357 | 0.015646 | 0.006300 | 0.000465 | 0.001241 |
Mitotic Spindle | 0.000334 | 0.000656 | 0.000756 | 0.006577 | 0.000139 | 0.000289 | 0.000117 | 0.000197 | 0.000158 | 0.000429 | 0.000180 | 0.001005 |
None | 0.000702 | 0.003106 | 0.000071 | 0.003888 | 0.007308 | 0.004650 | 0.002153 | 0.001515 | 0.006661 | 0.004059 | 0.001950 | 0.001816 |
Nuclear Periphery | 0.005600 | 0.003423 | 0.014505 | 0.000462 | 0.003619 | 0.001851 | 0.000902 | 0.000272 | 0.003813 | 0.002452 | 0.002231 | 0.000296 |
Nuclear Periphery Foci | 0.026144 | 0.007399 | 0.096561 | 0.019609 | 0.005020 | 0.003580 | 0.008428 | 0.004074 | 0.007087 | 0.005041 | 0.017042 | 0.006040 |
Nucleolus | 0.000055 | 0.000134 | 0.000074 | 0.000073 | 0.000410 | 0.000141 | 0.000020 | 0.000037 | 0.000375 | 0.000139 | 0.000026 | 0.000041 |
Nucleus | 0.009071 | 0.010366 | 0.010633 | 0.005972 | 0.007029 | 0.014115 | 0.004594 | 0.002623 | 0.007229 | 0.012681 | 0.005185 | 0.003047 |
Peroxisomes | 0.000629 | 0.002546 | 0.000325 | 0.000597 | 0.009564 | 0.003151 | 0.000164 | 0.001301 | 0.008690 | 0.002919 | 0.000180 | 0.001212 |
Vacuole | 0.056415 | 0.010316 | 0.052022 | 0.034861 | 0.006155 | 0.010401 | 0.006296 | 0.008370 | 0.011072 | 0.010369 | 0.010765 | 0.011722 |
Vacuole Periphery | 0.002782 | 0.001670 | 0.000338 | 0.000378 | 0.003413 | 0.002648 | 0.000167 | 0.000686 | 0.003351 | 0.002274 | 0.000183 | 0.000647 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.01 | -1.64 | -2.09 | -2.09 | -1.04 | -0.66 | -1.85 | -5.00 | -4.45 | -1.87 | -1.23 | -2.25 | -5.19 | -4.59 | -2.21 |
Bud Neck | 0.39 | -2.87 | -3.30 | -3.47 | -0.63 | -0.53 | 1.82 | -1.70 | -1.12 | -2.85 | 0.04 | 0.94 | -2.56 | -2.53 | -3.07 |
Bud Site | 1.00 | -0.95 | -2.06 | -3.31 | -0.91 | 3.00 | 1.86 | -3.75 | -5.67 | -4.87 | 3.03 | 1.32 | -4.26 | -6.34 | -5.01 |
Cell Periphery | 1.19 | 0.66 | -1.51 | -3.77 | -2.85 | 4.56 | 3.36 | 0.46 | -7.71 | -4.61 | 4.32 | 3.41 | -0.15 | -7.57 | -5.44 |
Cytoplasm | -6.79 | 0.17 | 0.41 | 8.42 | 0.25 | -2.09 | 19.29 | 12.92 | 14.84 | -5.88 | -2.28 | 18.44 | 12.71 | 16.56 | -5.41 |
Cytoplasmic Foci | -4.23 | -0.68 | 1.37 | 7.86 | 2.29 | 5.67 | 17.84 | 15.34 | 12.21 | -5.95 | 4.31 | 17.16 | 15.24 | 15.62 | -4.23 |
Eisosomes | 0.38 | 1.53 | 0.09 | -0.56 | -2.96 | 2.10 | 4.83 | 3.65 | 3.29 | -3.79 | 2.32 | 5.04 | 3.54 | 2.91 | -4.62 |
Endoplasmic Reticulum | 1.96 | 3.06 | 0.73 | -1.21 | -2.54 | -2.58 | -25.96 | -17.66 | -15.74 | 8.48 | -2.89 | -22.83 | -16.08 | -15.59 | 6.84 |
Endosome | 1.50 | -3.11 | 0.16 | -1.78 | 3.83 | 0.69 | 8.03 | 1.96 | 1.24 | -6.85 | 0.81 | 5.87 | 1.71 | 1.05 | -4.67 |
Golgi | -7.31 | 1.79 | -0.36 | 8.02 | -3.21 | 2.83 | 17.01 | 13.86 | 11.76 | -6.71 | 2.23 | 17.00 | 13.67 | 14.30 | -7.34 |
Lipid Particles | -3.72 | -0.87 | -3.97 | -1.95 | -3.64 | 0.19 | 15.92 | 8.02 | 8.29 | -8.34 | 0.04 | 15.28 | 5.98 | 6.77 | -9.25 |
Mitochondria | -6.70 | 1.74 | -1.16 | 6.07 | -2.04 | 5.87 | 12.37 | 11.57 | 7.00 | -3.41 | 6.86 | 12.32 | 11.53 | 8.11 | -3.53 |
Mitotic Spindle | -0.81 | -1.48 | -2.02 | -1.95 | -1.92 | -1.35 | 0.28 | -1.12 | 0.69 | -1.48 | -1.80 | -0.60 | -2.11 | -1.64 | -2.06 |
None | -2.37 | 1.06 | -0.91 | -0.20 | -1.11 | 1.89 | 4.27 | 5.12 | 3.26 | 1.00 | 2.22 | 4.31 | 4.40 | 2.74 | 0.20 |
Nuclear Periphery | 0.83 | -2.45 | 2.14 | 2.92 | 5.07 | 2.33 | 3.69 | 4.59 | 7.14 | 6.89 | 1.69 | 2.09 | 5.03 | 5.31 | 6.61 |
Nuclear Periphery Foci | 4.35 | -7.58 | 0.79 | -3.79 | 8.27 | 2.28 | -3.50 | 1.12 | -1.15 | 4.59 | 2.63 | -7.13 | 0.74 | -1.84 | 7.77 |
Nucleolus | -3.56 | -0.93 | -0.75 | 1.42 | -0.13 | 3.09 | 4.53 | 4.31 | 6.81 | -2.97 | 3.02 | 4.49 | 4.25 | 7.65 | -2.56 |
Nucleus | -0.36 | -0.66 | 0.63 | 1.05 | 1.22 | -6.97 | 3.08 | 7.23 | 12.28 | 3.85 | -6.61 | 2.41 | 5.62 | 11.83 | 3.35 |
Peroxisomes | -7.33 | 2.05 | 0.24 | 7.73 | -1.96 | 8.57 | 13.13 | 11.35 | 7.23 | -8.21 | 8.64 | 13.12 | 11.35 | 8.60 | -8.44 |
Vacuole | 5.19 | -0.07 | 1.84 | -4.47 | 2.14 | -4.40 | -0.90 | -2.70 | 1.55 | -1.44 | 0.79 | -0.55 | -0.71 | -1.70 | -0.13 |
Vacuole Periphery | 0.53 | 1.19 | 1.17 | 3.63 | -0.36 | 1.15 | 10.05 | 7.52 | 3.20 | -2.92 | 2.07 | 8.98 | 7.09 | 3.92 | -2.96 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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