Standard name
Human Ortholog
Description Guanine nucleotide exchange factor (GEF); activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER; SEC12 has a paralog, SED4, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0.25 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0.05 0 0.07 0.06 0.05 0.07 0.05 0.06 0 0.06 0.06 0.08 0.08 0.08 0.1 – 0.11 0.12 0 0 0 0 0 0
Cytoplasm 0.15 0.29 0.2 0.18 0.32 0.32 0.33 0.3 0.3 0.32 0.29 0.32 0.54 0.53 0.5 – 0.72 0.44 0 0.06 0 0 0 0
Endoplasmic Reticulum 0.82 0.7 0.73 0.5 0.27 0.3 0.24 0.26 0.26 0.14 0.12 0.14 0.25 0.25 0.44 – 0 0 0.89 0.8 0.87 0.74 0.79 0.8
Endosome 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 – 0.06 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.12 0 0 0 0 0 0
Mitochondria 0 0 0.1 0.36 0.21 0.4 0.35 0.42 0.52 0.56 0.62 0.49 0 0 0 – 0 0 0 0 0 0.1 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.09 0.29 0.15 0.23 0.16 0.08 0.15 0.08 0.15 0.17 0.19 0.05 – 0.06 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 12 14 14 2 2 2 6 5 1 2 3 11 11 8 – 1 4 3 7 15 12 19 14
Bud 0 2 0 4 0 10 5 12 9 13 15 15 2 2 0 – 0 0 0 1 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 2 1 0 3 4 5
Bud Site 0 0 0 0 0 0 0 0 0 1 2 1 0 0 0 – 0 0 – – – – – –
Cell Periphery 8 4 27 18 12 24 21 30 13 23 21 29 21 21 27 – 2 2 1 2 10 10 19 24
Cytoplasm 25 65 78 51 83 107 141 159 102 130 111 119 141 141 142 – 13 7 3 15 1 12 23 17
Endoplasmic Reticulum 140 156 278 139 70 103 100 136 87 58 47 54 66 65 123 – 0 0 181 198 368 268 462 473
Endosome 0 0 3 7 16 10 10 15 3 3 2 9 1 1 0 – 1 1 0 2 1 2 2 3
Golgi 0 1 1 2 0 0 0 0 0 0 0 0 0 0 0 – 0 2 0 1 2 1 2 2
Mitochondria 6 9 39 100 56 136 149 222 174 225 237 185 2 2 0 – 0 0 4 6 10 34 30 23
Nucleus 0 1 0 0 0 0 3 4 0 1 1 1 2 2 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 1 0 5 1 1 1 7 10 9 8 2 10 0 0 0 – 0 0 0 0 2 0 1 0
Nucleolus 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 1 1 0 0 1 0 1 0 1 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 1 0 0 2 1 1 0 – 0 0 1 0 0 0 0 0
Vac/Vac Membrane 7 9 16 24 75 52 99 87 28 61 32 55 45 50 15 – 1 1 4 7 1 12 16 20
Unique Cell Count 170 222 383 277 262 339 424 532 336 404 381 376 260 265 282 18 16 204 250 422 362 587 593
Labelled Cell Count 192 259 461 361 316 445 537 683 431 527 472 484 292 296 315 18 17 204 250 422 362 587 593


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.0 7.5 6.9 7.0 6.9 5.8 5.7 5.6 5.0 5.2 4.9 5.5 7.8 7.8 7.8 – 9.9 10.5 8.1 8.5 8.8
Std Deviation (1e-4) 0.8 1.4 1.3 1.3 1.4 1.5 1.4 1.1 1.2 1.1 1.1 0.9 1.0 1.0 0.9 – 2.6 2.4 1.2 1.4 1.3
Intensity Change (Log2) – – – 0.01 -0.0 -0.26 -0.28 -0.32 -0.46 -0.41 -0.5 -0.33 0.16 0.16 0.16 – 0.52 0.6 0.22 0.3 0.35

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.9 -2.3 -2.8 -3.2 -2.6 -1.8 -3.5 -3.0 -2.7 0.4 0.3 -0.6 – 0 0
Bud 0 0 0 0 3.0 0 3.5 3.9 3.9 0 0 0 – 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Cell Periphery -0.3 -1.3 0 -1.3 -0.9 -1.9 -0.8 -0.9 0.3 0.5 0.4 1.2 – 0 0
Cytoplasm -0.6 3.3 3.4 4.1 3.2 3.1 3.8 2.8 3.5 8.9 8.7 8.1 – 0 0
Endoplasmic Reticulum -5.9 -11.5 -11.3 -13.9 -14.1 -12.5 -16.5 -16.8 -16.2 -11.8 -12.0 -7.5 – -6.5 -6.2
Endosome 0 3.9 2.2 1.8 2.2 0 0 0 1.8 0 0 0 – 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Mitochondria 8.1 3.9 9.4 8.4 10.4 12.2 13.5 15.0 11.8 -4.8 -4.8 -5.5 – 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Nuclear Periphery 0 0 0 0.4 0.7 1.3 0.7 0 1.3 0 0 0 – 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Vacuole 2.4 8.8 5.1 7.8 5.7 2.3 5.2 2.4 4.9 5.6 6.1 0.7 – 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.4842 4.9107 4.7924 4.184 4.3659 5.5713 5.484 6.4121 5.9177 5.4092 5.0585 5.7204 6.7559 7.0964 6.6965 6.2452 5.923 6.52
Actin 0.014 0.0024 0.0053 0.0091 0.0044 0.0029 0.0316 0.0022 0.0016 0.0001 0.0018 0.0032 0.0057 0.002 0.0015 0.0006 0.0001 0.0012
Bud 0.0003 0.0016 0.0003 0.0171 0.0005 0.0001 0.0002 0 0.0002 0 0.0001 0 0 0.0002 0.0001 0 0 0
Bud Neck 0.001 0.0007 0.0017 0.0013 0.0006 0.0025 0.0008 0.0002 0.0003 0.0001 0.0002 0.0006 0.0003 0.0005 0.0008 0.0005 0.0002 0.0016
Bud Periphery 0.0015 0.0007 0.0009 0.017 0.0009 0.0002 0.0003 0.0001 0.0005 0 0.0002 0.0002 0.0001 0.0006 0.0001 0.0001 0 0
Bud Site 0.0016 0.0044 0.009 0.0191 0.0062 0.0001 0.005 0.0025 0.001 0 0.0007 0.0001 0.0003 0.006 0.0007 0 0 0.0001
Cell Periphery 0.0049 0.0081 0.0096 0.0017 0.001 0.0029 0.0026 0.0016 0.0025 0.0014 0.0017 0.0011 0.0025 0.0059 0.0021 0.0018 0.0009 0.0011
Cytoplasm 0.1341 0.2506 0.212 0.1791 0.1295 0.2355 0.0429 0.1083 0.0876 0.0315 0.0653 0.0724 0.0908 0.0711 0.0625 0.0628 0.0275 0.1065
Cytoplasmic Foci 0.0166 0.0099 0.0206 0.0127 0.0086 0.0038 0.0097 0.0029 0.0075 0.0024 0.0121 0.005 0.0114 0.0026 0.0052 0.0009 0.0006 0.0044
Eisosomes 0.0008 0.0002 0.0002 0.0002 0.0001 0.0001 0.0004 0.0001 0 0 0.0004 0.0006 0.0003 0.0001 0.0001 0 0 0
Endoplasmic Reticulum 0.6243 0.5944 0.6241 0.6535 0.726 0.6755 0.8035 0.8531 0.8654 0.9061 0.8296 0.8762 0.8182 0.8855 0.8738 0.9099 0.9617 0.8616
Endosome 0.026 0.016 0.0219 0.0186 0.0096 0.0166 0.0214 0.01 0.0083 0.0092 0.0193 0.0086 0.0129 0.0055 0.0172 0.0037 0.0012 0.006
Golgi 0.0094 0.0021 0.0054 0.0058 0.0019 0.0052 0.0139 0.0016 0.0012 0.0009 0.0117 0.0048 0.0034 0.0016 0.0028 0.0007 0.0003 0.0011
Lipid Particles 0.0279 0.0123 0.0157 0.0036 0.0088 0.0037 0.0155 0.0036 0.0072 0.0165 0.0165 0.012 0.0116 0.01 0.0187 0.0052 0.0036 0.0038
Mitochondria 0.0056 0.0009 0.0046 0.0127 0.0022 0.0023 0.0048 0.0004 0.0004 0.0001 0.0069 0.0012 0.001 0.0002 0.0006 0.0002 0 0.0001
None 0.0642 0.0461 0.0107 0.0099 0.0029 0.0242 0.0212 0.0026 0.0054 0.0006 0.0232 0.003 0.0303 0.0006 0.0009 0.0047 0.0002 0.0064
Nuclear Periphery 0.0374 0.0161 0.0082 0.011 0.0159 0.008 0.0071 0.0055 0.004 0.026 0.0037 0.0036 0.0041 0.0042 0.0063 0.0062 0.0024 0.0032
Nucleolus 0.0006 0.0154 0.0209 0.0007 0.0066 0 0.0002 0 0.0001 0 0.0001 0 0.0001 0 0.0002 0 0 0
Nucleus 0.012 0.0089 0.0141 0.0065 0.0479 0.0016 0.0012 0.001 0.0006 0.0004 0.0005 0.0006 0.0004 0.0005 0.0011 0.0004 0.0002 0.0005
Peroxisomes 0.0049 0.0006 0.0046 0.0008 0.0176 0.0003 0.0095 0.0001 0.0025 0.0001 0.002 0.004 0.0021 0 0.0002 0 0 0.0001
Punctate Nuclear 0.0051 0.0021 0.004 0.0106 0.0047 0.0003 0.0043 0.0002 0.0002 0.0021 0.0016 0.0002 0.002 0.0002 0.0009 0.0001 0 0.0002
Vacuole 0.0057 0.006 0.0056 0.007 0.0035 0.0102 0.0031 0.0036 0.0033 0.002 0.0017 0.0023 0.0021 0.0025 0.0036 0.0019 0.001 0.0019
Vacuole Periphery 0.0019 0.0006 0.0008 0.0023 0.0005 0.0042 0.0006 0.0003 0.0002 0.0005 0.0006 0.0003 0.0003 0.0001 0.0006 0.0003 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.8187 18.808 20.1592 22.0175 17.1754 16.355 18.6646 20.9164 20.8438 26.6798
Translational Efficiency 2.1175 1.4837 1.3366 1.1948 1.8674 2.7758 1.6556 1.27 1.5338 1.6304

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
185 1211 220 275 1706 1955 2031 1898 1891 3166 2251 2173

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1048.10 954.52 1229.16 1233.97 803.78 932.09 1159.43 1119.66 827.68 940.67 1166.25 1134.13
Standard Deviation 125.70 162.20 135.33 165.41 124.78 124.42 150.27 156.62 144.44 140.50 150.31 162.28
Intensity Change Log 2 — -0.134929 0.229896 0.235531 — 0.213669 0.528543 0.478188 — 0.026806 0.367169 0.345897

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001310 0.001354 0.003745 0.006936 0.000842 0.000973 0.001776 0.003020 0.000888 0.001119 0.001968 0.003516
Bud Neck 0.001504 0.001413 0.004059 0.004813 0.001915 0.002144 0.001198 0.002706 0.001875 0.001864 0.001477 0.002973
Bud Site 0.007720 0.005256 0.012624 0.017087 0.007555 0.004402 0.005100 0.013942 0.007571 0.004729 0.005835 0.014340
Cell Periphery 0.005584 0.002571 0.003744 0.010327 0.006004 0.001609 0.002444 0.005493 0.005963 0.001977 0.002571 0.006105
Cytoplasm 0.059090 0.130657 0.053328 0.053362 0.141843 0.154220 0.051411 0.073831 0.133747 0.145207 0.051599 0.071241
Cytoplasmic Foci 0.015663 0.033893 0.018183 0.009695 0.043623 0.027633 0.001883 0.006407 0.040888 0.030027 0.003476 0.006823
Eisosomes 0.000945 0.000765 0.000246 0.000909 0.001441 0.000890 0.000238 0.000518 0.001393 0.000842 0.000239 0.000567
Endoplasmic Reticulum 0.773214 0.718912 0.686531 0.760237 0.664223 0.693094 0.904163 0.844469 0.674886 0.702969 0.882893 0.833809
Endosome 0.010446 0.007709 0.018240 0.009798 0.007443 0.006945 0.002765 0.006036 0.007737 0.007237 0.004278 0.006512
Golgi 0.003916 0.018439 0.001517 0.004488 0.024508 0.019389 0.001046 0.004367 0.022494 0.019026 0.001092 0.004382
Lipid Particles 0.018640 0.035523 0.022398 0.049486 0.040628 0.040082 0.004629 0.018779 0.038477 0.038338 0.006365 0.022665
Mitochondria 0.000236 0.003891 0.000100 0.000444 0.017317 0.007792 0.000505 0.001357 0.015646 0.006300 0.000465 0.001241
Mitotic Spindle 0.000334 0.000656 0.000756 0.006577 0.000139 0.000289 0.000117 0.000197 0.000158 0.000429 0.000180 0.001005
None 0.000702 0.003106 0.000071 0.003888 0.007308 0.004650 0.002153 0.001515 0.006661 0.004059 0.001950 0.001816
Nuclear Periphery 0.005600 0.003423 0.014505 0.000462 0.003619 0.001851 0.000902 0.000272 0.003813 0.002452 0.002231 0.000296
Nuclear Periphery Foci 0.026144 0.007399 0.096561 0.019609 0.005020 0.003580 0.008428 0.004074 0.007087 0.005041 0.017042 0.006040
Nucleolus 0.000055 0.000134 0.000074 0.000073 0.000410 0.000141 0.000020 0.000037 0.000375 0.000139 0.000026 0.000041
Nucleus 0.009071 0.010366 0.010633 0.005972 0.007029 0.014115 0.004594 0.002623 0.007229 0.012681 0.005185 0.003047
Peroxisomes 0.000629 0.002546 0.000325 0.000597 0.009564 0.003151 0.000164 0.001301 0.008690 0.002919 0.000180 0.001212
Vacuole 0.056415 0.010316 0.052022 0.034861 0.006155 0.010401 0.006296 0.008370 0.011072 0.010369 0.010765 0.011722
Vacuole Periphery 0.002782 0.001670 0.000338 0.000378 0.003413 0.002648 0.000167 0.000686 0.003351 0.002274 0.000183 0.000647

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.01 -1.64 -2.09 -2.09 -1.04 -0.66 -1.85 -5.00 -4.45 -1.87 -1.23 -2.25 -5.19 -4.59 -2.21
Bud Neck 0.39 -2.87 -3.30 -3.47 -0.63 -0.53 1.82 -1.70 -1.12 -2.85 0.04 0.94 -2.56 -2.53 -3.07
Bud Site 1.00 -0.95 -2.06 -3.31 -0.91 3.00 1.86 -3.75 -5.67 -4.87 3.03 1.32 -4.26 -6.34 -5.01
Cell Periphery 1.19 0.66 -1.51 -3.77 -2.85 4.56 3.36 0.46 -7.71 -4.61 4.32 3.41 -0.15 -7.57 -5.44
Cytoplasm -6.79 0.17 0.41 8.42 0.25 -2.09 19.29 12.92 14.84 -5.88 -2.28 18.44 12.71 16.56 -5.41
Cytoplasmic Foci -4.23 -0.68 1.37 7.86 2.29 5.67 17.84 15.34 12.21 -5.95 4.31 17.16 15.24 15.62 -4.23
Eisosomes 0.38 1.53 0.09 -0.56 -2.96 2.10 4.83 3.65 3.29 -3.79 2.32 5.04 3.54 2.91 -4.62
Endoplasmic Reticulum 1.96 3.06 0.73 -1.21 -2.54 -2.58 -25.96 -17.66 -15.74 8.48 -2.89 -22.83 -16.08 -15.59 6.84
Endosome 1.50 -3.11 0.16 -1.78 3.83 0.69 8.03 1.96 1.24 -6.85 0.81 5.87 1.71 1.05 -4.67
Golgi -7.31 1.79 -0.36 8.02 -3.21 2.83 17.01 13.86 11.76 -6.71 2.23 17.00 13.67 14.30 -7.34
Lipid Particles -3.72 -0.87 -3.97 -1.95 -3.64 0.19 15.92 8.02 8.29 -8.34 0.04 15.28 5.98 6.77 -9.25
Mitochondria -6.70 1.74 -1.16 6.07 -2.04 5.87 12.37 11.57 7.00 -3.41 6.86 12.32 11.53 8.11 -3.53
Mitotic Spindle -0.81 -1.48 -2.02 -1.95 -1.92 -1.35 0.28 -1.12 0.69 -1.48 -1.80 -0.60 -2.11 -1.64 -2.06
None -2.37 1.06 -0.91 -0.20 -1.11 1.89 4.27 5.12 3.26 1.00 2.22 4.31 4.40 2.74 0.20
Nuclear Periphery 0.83 -2.45 2.14 2.92 5.07 2.33 3.69 4.59 7.14 6.89 1.69 2.09 5.03 5.31 6.61
Nuclear Periphery Foci 4.35 -7.58 0.79 -3.79 8.27 2.28 -3.50 1.12 -1.15 4.59 2.63 -7.13 0.74 -1.84 7.77
Nucleolus -3.56 -0.93 -0.75 1.42 -0.13 3.09 4.53 4.31 6.81 -2.97 3.02 4.49 4.25 7.65 -2.56
Nucleus -0.36 -0.66 0.63 1.05 1.22 -6.97 3.08 7.23 12.28 3.85 -6.61 2.41 5.62 11.83 3.35
Peroxisomes -7.33 2.05 0.24 7.73 -1.96 8.57 13.13 11.35 7.23 -8.21 8.64 13.12 11.35 8.60 -8.44
Vacuole 5.19 -0.07 1.84 -4.47 2.14 -4.40 -0.90 -2.70 1.55 -1.44 0.79 -0.55 -0.71 -1.70 -0.13
Vacuole Periphery 0.53 1.19 1.17 3.63 -0.36 1.15 10.05 7.52 3.20 -2.92 2.07 8.98 7.09 3.92 -2.96
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Guanine nucleotide exchange factor (GEF); activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER; SEC12 has a paralog, SED4, that arose from the whole genome duplication
Localization
Cell Percentages ER (90%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Sec12

Sec12


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sec12-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available