ORF
Human Ortholog
Description Putative protein of unknown function; deletion confers reduced fitness in saline
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.98 0.99 0.98 0.92 0.84 0.83 0.75 0.76 0.63 0.68 1.0 0.99 1.0 0.95 0.9 0.98 0.91 0.93 0.91 0.95 0.88 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.08 0.05 0.27 0.37 0.32 0.4 0.45 0.59 0.54 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 2
Bud 1 1 1 0 2 7 27 12 8 10 11 9 0 0 0 0 0 0 0 4 4 1 6 3
Bud Neck 0 0 1 0 0 0 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 3 3 1 1 7 3 3 3 3 1 1 1 1 3 6 4 0 0 0 0 0 0
Cytoplasm 161 300 321 275 311 421 564 429 345 340 232 246 211 253 352 81 121 96 185 280 283 208 279 299
Endoplasmic Reticulum 0 2 4 1 2 1 0 0 2 0 1 2 0 0 1 6 5 1 3 0 3 0 2 0
Endosome 0 0 1 0 0 2 0 1 3 2 0 2 0 1 0 1 3 0 1 3 3 1 3 3
Golgi 0 0 1 0 0 1 0 0 0 0 0 0 0 1 0 1 1 1 0 2 2 0 1 1
Mitochondria 5 10 0 22 17 123 249 163 184 199 217 195 0 0 1 3 4 1 6 4 2 0 2 2
Nucleus 0 0 0 0 0 0 5 1 1 0 2 2 1 4 0 1 3 1 0 0 1 1 4 2
Nuclear Periphery 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 1 0
Nucleolus 0 0 1 1 0 0 1 0 0 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 2 0 1 2 2 2 3 4 0 0 0 0 0 0 0 1 1 0 3 2
Vac/Vac Membrane 0 0 0 0 0 6 20 6 18 3 14 8 0 0 1 0 6 0 0 2 2 0 3 1
Unique Cell Count 165 305 326 278 318 460 674 514 459 445 367 361 212 255 353 85 134 98 203 302 313 218 319 324
Labelled Cell Count 169 313 333 302 335 562 878 618 566 560 484 471 213 260 356 96 149 105 203 302 313 218 319 324


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 6.0 5.2 4.5 4.8 3.8 3.6 3.4 3.4 3.2 3.0 3.1 6.6 6.7 6.4 9.1 8.6 8.5 6.5 7.2 7.1
Std Deviation (1e-4) 1.0 1.4 1.5 1.2 1.6 1.5 1.4 1.0 1.2 0.9 1.0 1.4 1.4 1.4 1.3 1.7 2.0 1.7 1.5 1.7 1.5
Intensity Change (Log2) – – – -0.2 -0.13 -0.47 -0.55 -0.63 -0.6 -0.69 -0.81 -0.75 0.34 0.36 0.28 0.8 0.73 0.71 0.32 0.46 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 -0.6 -4.2 -6.9 -6.9 -9.0 -8.7 -11.5 -10.5 1.1 0.8 1.7 -1.8 -4.1 -0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.1 0 3.6 0 3.6 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.7371 4.7683 4.8485 4.2466 4.3137 4.6446 0.5249 0.9299 1.2904 1.5726 0.2897 1.6934 0.9748 1.7202 1.0565 1.9886 0.93 0.9793
Actin 0.0601 0.0046 0.018 0.0228 0.0116 0.009 0.0538 0.0044 0.0066 0.2229 0.0014 0.0227 0.0186 0.0008 0.0214 0.0167 0.0026 0.0091
Bud 0.0007 0.0017 0.0009 0.0004 0.0116 0.0003 0.0008 0.0005 0.0058 0.0492 0.0001 0.0124 0.0054 0.0153 0.0048 0.0003 0.0008 0.0027
Bud Neck 0.0136 0.0003 0.0014 0.0009 0.0005 0.0005 0.003 0.0002 0.0031 0.0014 0.0004 0.0041 0.003 0.0002 0.0007 0.0055 0.0004 0.0034
Bud Periphery 0.0016 0.0011 0.0008 0.0011 0.0277 0.001 0.0015 0.0007 0.0039 0.0152 0 0.0062 0.0019 0.0036 0.0011 0.0004 0.0006 0.0022
Bud Site 0.0057 0.0059 0.0055 0.0023 0.0006 0.0002 0.0028 0.0108 0.0049 0.0197 0 0.0046 0.0053 0.0064 0.003 0.0017 0.0004 0.0007
Cell Periphery 0.001 0.0003 0.0004 0.0006 0.0003 0.0001 0.0011 0.0002 0.0003 0.0012 0 0.0003 0.0004 0.0003 0.0002 0.0003 0 0.0005
Cytoplasm 0.3573 0.5044 0.5001 0.492 0.5551 0.6095 0.3429 0.6876 0.6474 0.3979 0.4438 0.7259 0.511 0.6263 0.5051 0.5332 0.6698 0.674
Cytoplasmic Foci 0.0264 0.0111 0.0163 0.0088 0.0082 0.0044 0.0485 0.0111 0.0198 0.0245 0.0906 0.0176 0.0177 0.0103 0.0115 0.0157 0.0488 0.0124
Eisosomes 0.0006 0.0001 0.0001 0.0012 0.0001 0.0001 0.0088 0.0001 0.0001 0.0007 0 0.0004 0.0001 0.0001 0.0002 0.0003 0 0
Endoplasmic Reticulum 0.006 0.0025 0.0023 0.0035 0.0021 0.0034 0.0075 0.0024 0.0019 0.0162 0.0023 0.0114 0.0037 0.0019 0.0035 0.0016 0.0022 0.0013
Endosome 0.0246 0.0044 0.0155 0.004 0.0019 0.0044 0.0431 0.0088 0.0167 0.085 0.0067 0.0205 0.0097 0.0009 0.0113 0.0091 0.0207 0.0013
Golgi 0.0051 0.0004 0.011 0.0039 0.0005 0.0012 0.0111 0.0037 0.0053 0.042 0.002 0.007 0.0028 0.0001 0.0038 0.0101 0.0113 0.0006
Lipid Particles 0.0061 0.0002 0.0054 0.0023 0.0002 0.0007 0.0116 0.0005 0.0006 0.0115 0.0274 0.0042 0.0045 0.0003 0.0039 0.0167 0.0064 0.0013
Mitochondria 0.0245 0.0004 0.0162 0.0351 0.0008 0.0036 0.0035 0.0047 0.0125 0.0351 0.0003 0.0161 0.0015 0.0009 0.0014 0.0108 0.0053 0.0091
None 0.4171 0.4496 0.3789 0.4091 0.3747 0.357 0.4183 0.2581 0.2483 0.0283 0.4103 0.1215 0.387 0.3268 0.3936 0.3457 0.2075 0.2702
Nuclear Periphery 0.0208 0.0019 0.001 0.0009 0.0007 0.0009 0.0036 0.0006 0.0012 0.0191 0.0005 0.0029 0.0065 0.0005 0.0099 0.0007 0.0014 0.0005
Nucleolus 0.0008 0.0001 0.0016 0.0002 0.0001 0.0001 0.0004 0.0002 0.0009 0.0013 0 0.0007 0.0004 0.0007 0.0004 0.0005 0.0004 0.0001
Nucleus 0.0042 0.0017 0.0015 0.0012 0.0016 0.0016 0.0017 0.0009 0.0029 0.0067 0.0011 0.003 0.0043 0.0021 0.0112 0.0011 0.0023 0.0017
Peroxisomes 0.0122 0.0019 0.0151 0.0078 0.0004 0.0005 0.0168 0.0008 0.0011 0.011 0.0119 0.0074 0.0063 0.0001 0.0023 0.0263 0.0017 0.0069
Punctate Nuclear 0.0053 0.0053 0.0025 0.0005 0.0007 0.0006 0.0164 0.0007 0.0037 0.0039 0.0008 0.0097 0.0077 0.0012 0.0062 0.0014 0.0157 0.0014
Vacuole 0.0045 0.0016 0.0045 0.0012 0.0006 0.0008 0.002 0.0018 0.0099 0.0037 0.0005 0.0011 0.0015 0.001 0.0038 0.0013 0.0008 0.0005
Vacuole Periphery 0.0017 0.0004 0.0008 0.0003 0.0002 0.0002 0.0008 0.0013 0.0031 0.0033 0.0001 0.0004 0.0009 0.0001 0.0007 0.0007 0.0006 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.2517 9.9402 11.9152 19.4036 20.5165 22.2268 17.0086 18.0022 17.1598 17.144
Translational Efficiency 1.7391 2.9504 2.1555 1.5679 1.4402 1.1822 1.4796 1.3067 1.2738 1.3742

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1667 1450 2617 1113 2242 1906 169 94 3909 3356 2786 1207

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 700.89 828.48 1008.86 908.93 748.80 780.44 1187.77 1160.77 728.37 801.20 1019.71 928.54
Standard Deviation 87.23 121.59 116.91 210.34 97.38 110.08 148.32 154.96 96.15 117.62 126.48 217.31
Intensity Change Log 2 — 0.241279 0.525466 0.374981 — 0.059707 0.665603 0.632430 — 0.150348 0.599547 0.513677

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000298 0.000207 0.000091 0.001688 0.000144 0.000198 0.000684 0.001157 0.000210 0.000202 0.000127 0.001647
Bud Neck 0.013181 0.007049 0.003212 0.004004 0.007958 0.013131 0.003554 0.070793 0.010185 0.010503 0.003232 0.009206
Bud Site 0.007163 0.007124 0.005261 0.043623 0.003743 0.008548 0.016915 0.074110 0.005201 0.007933 0.005968 0.045997
Cell Periphery 0.000166 0.000077 0.000053 0.000755 0.000097 0.000149 0.000205 0.000156 0.000126 0.000118 0.000062 0.000708
Cytoplasm 0.529699* 0.655940* 0.607814* 0.655886* 0.626248* 0.600977* 0.529224* 0.221838* 0.585075* 0.624724* 0.603046* 0.622082*
Cytoplasmic Foci 0.250925* 0.148647 0.005714 0.018503 0.132899 0.157344 0.025548 0.020166 0.183231* 0.153587 0.006917 0.018633
Eisosomes 0.000238 0.000050 0.000013 0.000034 0.000071 0.000073 0.000059 0.000049 0.000142 0.000063 0.000016 0.000035
Endoplasmic Reticulum 0.000805 0.000694 0.001576 0.001607 0.001540 0.000720 0.004059 0.002169 0.001227 0.000709 0.001726 0.001650
Endosome 0.009132 0.010103 0.000978 0.005950 0.004168 0.012447 0.006855 0.069173 0.006285 0.011434 0.001335 0.010874
Golgi 0.003497 0.001625 0.000065 0.005019 0.001348 0.002701 0.000206 0.005917 0.002264 0.002236 0.000074 0.005089
Lipid Particles 0.005832 0.001869 0.000456 0.002030 0.003597 0.002971 0.002334 0.001827 0.004550 0.002495 0.000570 0.002014
Mitochondria 0.006490 0.001762 0.000400 0.003518 0.003442 0.005484 0.000184 0.003124 0.004742 0.003876 0.000387 0.003487
Mitotic Spindle 0.002676 0.003768 0.005337 0.018674 0.002792 0.009273 0.002895 0.042585 0.002743 0.006894 0.005189 0.020536
None 0.003034 0.003523 0.005310 0.006394 0.003224 0.003991 0.007774 0.001014 0.003143 0.003789 0.005460 0.005975
Nuclear Periphery 0.000233 0.000269 0.001236 0.000595 0.000343 0.000277 0.001248 0.000663 0.000296 0.000274 0.001236 0.000600
Nuclear Periphery Foci 0.000349 0.000803 0.000762 0.004370 0.000353 0.000353 0.009227 0.002613 0.000351 0.000548 0.001276 0.004233
Nucleolus 0.001245 0.000724 0.000149 0.000703 0.000448 0.000892 0.000178 0.000887 0.000788 0.000819 0.000151 0.000717
Nucleus 0.114948 0.087249 0.311769* 0.097678 0.167460* 0.098836 0.192224* 0.027252 0.145066* 0.093830 0.304518* 0.092193
Peroxisomes 0.006742 0.003557 0.000250 0.001523 0.001312 0.004237 0.000851 0.004505 0.003628 0.003943 0.000287 0.001755
Vacuole 0.041805 0.064138 0.049457 0.125284 0.038171 0.076100 0.195570 0.403514* 0.039721 0.070932 0.058320 0.146953
Vacuole Periphery 0.001542 0.000823 0.000097 0.002164 0.000640 0.001297 0.000206 0.046489 0.001024 0.001092 0.000103 0.005616

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.87 1.83 -3.68 -4.58 -5.13 -0.73 -4.46 -2.60 -2.10 1.72 0.02 0.67 -4.61 -4.72 -5.03
Bud Neck 5.86 9.91 11.37 6.53 1.78 -3.72 4.93 0.35 1.42 -0.66 -0.23 9.60 10.02 9.07 1.04
Bud Site 0.02 3.29 -5.16 -5.23 -6.37 -5.16 -1.85 -2.17 -0.93 -0.33 -4.26 1.04 -6.21 -5.01 -6.39
Cell Periphery 7.42 9.34 -1.36 -2.24 -2.43 -3.73 -6.11 0.48 3.52 5.63 0.54 10.44 -1.72 -1.78 -2.37
Cytoplasm -12.64 2.76 0.19 11.16 -2.09 3.57 13.63 12.46 11.26 1.82 -5.66 13.74 8.21 11.65 -1.22
Cytoplasmic Foci 14.52 46.67 43.97 27.94 -12.97 -4.54 28.79 27.65 29.24 0.38 6.86 57.01 52.13 43.22 -12.49
Eisosomes 4.90 5.72 5.35 5.50 -6.77 -0.37 0.66 3.43 3.99 2.39 4.77 7.29 6.50 10.56 -6.36
Endoplasmic Reticulum 0.59 -4.88 -4.18 -4.66 2.41 2.17 -6.41 -0.63 -2.51 5.62 2.25 -3.90 -1.51 -3.85 3.18
Endosome -1.00 13.12 9.15 9.25 -3.44 -9.77 -1.40 -0.70 1.75 -0.42 -8.09 15.80 6.52 12.16 -3.23
Golgi 4.16 8.46 2.74 0.26 -2.09 -2.73 4.45 1.93 4.02 -0.94 0.20 9.61 1.45 1.32 -2.14
Lipid Particles 10.32 15.42 10.56 0.73 -5.06 1.35 2.90 5.08 4.73 2.94 6.82 15.05 9.12 3.34 -4.85
Mitochondria 4.54 6.06 4.79 0.94 -1.20 -1.51 4.01 3.86 4.92 -0.20 1.12 6.91 4.69 3.56 -1.20
Mitotic Spindle -0.77 -2.37 -4.17 -3.53 -2.75 -3.67 -0.45 -2.06 -1.43 -1.98 -3.54 -2.62 -4.75 -2.45 -3.29
None -0.28 -2.98 -0.71 -0.55 3.97 -0.96 -1.49 2.09 2.11 2.55 -0.95 -5.49 -0.88 0.27 4.46
Nuclear Periphery -1.11 -32.66 -8.45 -7.03 15.01 1.89 -12.15 -8.57 -9.26 4.21 0.67 -32.54 -8.55 -8.75 15.44
Nuclear Periphery Foci -4.53 -8.27 -11.50 -9.33 -8.58 -0.16 -8.00 -8.18 -8.14 5.19 -4.13 -11.72 -12.40 -11.43 -7.11
Nucleolus 1.60 3.27 2.87 3.72 -1.25 -3.66 6.71 4.83 5.55 0.54 -0.40 4.26 3.19 5.05 -1.22
Nucleus 5.92 -44.45 -1.23 -6.85 41.76 15.06 -5.09 10.28 2.98 9.62 15.51 -42.25 6.53 -5.48 42.90
Peroxisomes 3.72 8.64 8.13 7.51 -1.91 -5.37 0.73 2.91 6.50 0.58 -0.75 9.62 8.62 9.38 -1.73
Vacuole -8.29 -14.60 -28.46 -24.47 -21.66 -13.19 -13.55 -16.67 -15.29 -6.02 -15.48 -19.35 -31.62 -26.90 -22.20
Vacuole Periphery 2.45 5.07 2.32 0.58 -2.18 -3.39 5.56 -0.84 -0.63 -1.00 -0.28 7.08 0.51 0.66 -2.26
ORF
Human Ortholog
Description Putative protein of unknown function; deletion confers reduced fitness in saline
Localization
Cell Percentages cytoplasm (76%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YNR029C

YNR029C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ynr029c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available