Standard name | |
---|---|
Human Ortholog | |
Description | Alpha-1,6-mannosyltransferase localized to the ER; responsible for addition of alpha-1,6 mannose to dolichol-linked Man7GlcNAc2; acts in the dolichol pathway for N-glycosylation; human homolog ALG12 complements yeast null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 8 | 6 | 7 | 5 | 2 | 3 | 9 | 7 | 8 | 8 | 7 | 12 | 6 | 4 | 2 | 4 | 4 | 3 | 3 | 8 | 3 | 20 | 10 |
Bud | 0 | 3 | 6 | 3 | 4 | 4 | 4 | 10 | 7 | 8 | 7 | 3 | 3 | 2 | 1 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 2 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 6 | 0 | 7 | 8 | 9 | 20 | 17 | 14 | 12 | 18 | 25 | 34 | 25 | 13 | 25 | 5 | 13 | 4 | 0 | 0 | 1 | 4 | 10 | 14 |
Cytoplasm | 15 | 23 | 35 | 37 | 38 | 42 | 63 | 87 | 32 | 72 | 68 | 66 | 33 | 26 | 37 | 9 | 28 | 15 | 5 | 7 | 2 | 5 | 7 | 4 |
Endoplasmic Reticulum | 125 | 86 | 117 | 83 | 89 | 92 | 166 | 126 | 104 | 113 | 86 | 128 | 365 | 137 | 168 | 2 | 0 | 2 | 162 | 116 | 247 | 300 | 378 | 427 |
Endosome | 0 | 0 | 11 | 7 | 21 | 9 | 9 | 12 | 1 | 4 | 4 | 4 | 3 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 3 | 7 |
Golgi | 1 | 0 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 2 | 2 | 6 |
Mitochondria | 2 | 11 | 69 | 109 | 67 | 150 | 211 | 223 | 181 | 256 | 176 | 264 | 0 | 0 | 0 | 4 | 15 | 5 | 5 | 8 | 7 | 21 | 30 | 20 |
Nucleus | 2 | 0 | 0 | 3 | 0 | 2 | 0 | 8 | 1 | 2 | 1 | 3 | 7 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 5 | 5 | 2 | 6 | 3 | 21 | 14 | 17 | 16 | 7 | 20 | 8 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 2 | 8 | 4 | 4 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 4 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 17 | 5 | 39 | 25 | 80 | 85 | 103 | 132 | 36 | 94 | 40 | 73 | 80 | 9 | 1 | 2 | 15 | 10 | 4 | 5 | 2 | 8 | 23 | 26 |
Unique Cell Count | 155 | 119 | 234 | 214 | 262 | 317 | 462 | 498 | 284 | 432 | 338 | 466 | 457 | 173 | 224 | 23 | 72 | 36 | 187 | 146 | 285 | 359 | 489 | 530 |
Labelled Cell Count | 174 | 141 | 298 | 286 | 321 | 411 | 599 | 640 | 398 | 595 | 424 | 603 | 537 | 195 | 240 | 26 | 81 | 41 | 187 | 146 | 285 | 359 | 489 | 530 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.1 | 6.0 | 7.0 | 7.5 | 7.6 | 6.7 | 6.3 | 6.4 | 6.1 | 5.6 | 6.3 | 5.8 | 9.1 | 8.6 | 9.5 | 7.2 | 7.8 | 9.3 | 9.1 | 9.4 | 9.7 |
Std Deviation (1e-4) | 1.0 | 0.8 | 1.4 | 1.4 | 1.5 | 1.6 | 1.2 | 1.4 | 1.3 | 1.2 | 2.6 | 1.3 | 1.1 | 1.2 | 1.4 | 1.9 | 1.6 | 3.0 | 1.6 | 1.8 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.1 | 0.12 | -0.06 | -0.15 | -0.13 | -0.21 | -0.32 | -0.16 | -0.28 | 0.38 | 0.28 | 0.44 | 0.03 | 0.16 | 0.41 | 0.37 | 0.41 | 0.47 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.4 | -0.5 | 0 | 0 | 0 | -0.1 | 0 | -0.2 | 0 | 0 | 0.5 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | -0.5 | -0.1 | 0 | -0.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.4 | 0.3 | 1.8 | 0.5 | -0.1 | 0.7 | 0.8 | 2.3 | 2.3 | 1.5 | 2.1 | 3.4 | 0 | 0 | 0 |
Cytoplasm | 0.7 | -0.1 | -0.6 | -0.5 | 0.9 | -1.2 | 0.6 | 1.6 | -0.3 | -3.2 | 0 | 0.5 | 0 | 4.4 | 3.8 |
Endoplasmic Reticulum | -2.4 | -3.6 | -5.0 | -3.6 | -6.6 | -3.1 | -6.2 | -6.0 | -5.9 | 8.1 | 6.0 | 5.5 | -3.8 | -7.6 | -5.0 |
Endosome | -0.8 | 1.5 | -1.2 | -2.1 | -1.7 | -3.3 | -3.1 | -2.6 | -3.3 | 0 | -2.4 | -3.3 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.6 | -1.0 | 4.2 | 4.1 | 3.9 | 7.8 | 7.3 | 5.4 | 6.8 | -12.2 | -7.8 | -8.8 | -1.2 | -1.4 | -2.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0.1 | 0 | 1.6 | 0.5 | 2.2 | 1.1 | 0 | 1.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.5 | 3.6 | 2.8 | 1.7 | 2.9 | -1.3 | 1.6 | -1.6 | -0.3 | 0.3 | -3.5 | -6.1 | 0 | 0.8 | 1.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.0002 | 5.207 | 4.9892 | 4.5465 | 4.1023 | 4.779 | 4.889 | 6.0446 | 5.6256 | 5.2558 | 4.6596 | 5.4625 | 5.9466 | 5.9405 | 5.4432 | 5.1365 | 5.6085 | 5.2117 |
Actin | 0.0067 | 0.0024 | 0.0022 | 0.0265 | 0.0005 | 0.0045 | 0.032 | 0.0002 | 0.0085 | 0.0003 | 0.0044 | 0.0009 | 0 | 0.0009 | 0.0088 | 0.0043 | 0.0006 | 0.0003 |
Bud | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0006 | 0 | 0 |
Bud Neck | 0.0006 | 0.0005 | 0.0002 | 0.0278 | 0.0098 | 0.0009 | 0.0002 | 0.0001 | 0.0003 | 0.0009 | 0.0046 | 0.0003 | 0 | 0.0003 | 0.0015 | 0.0011 | 0.0002 | 0.0008 |
Bud Periphery | 0.0002 | 0.0004 | 0.0001 | 0.001 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0064 | 0 | 0 |
Bud Site | 0.0002 | 0.0024 | 0.0005 | 0.0004 | 0.0003 | 0.0001 | 0.0016 | 0.0004 | 0.0011 | 0 | 0.0001 | 0 | 0 | 0.0091 | 0.0006 | 0.0008 | 0 | 0 |
Cell Periphery | 0.0011 | 0.0038 | 0.0008 | 0.0015 | 0.0006 | 0.0006 | 0.0017 | 0.0035 | 0.0013 | 0.0013 | 0.0009 | 0.0002 | 0.0007 | 0.002 | 0.0029 | 0.016 | 0.0005 | 0.0006 |
Cytoplasm | 0.0562 | 0.0989 | 0.0477 | 0.0237 | 0.0957 | 0.0661 | 0.019 | 0.0568 | 0.0341 | 0.0028 | 0.0466 | 0.0343 | 0.0074 | 0.0596 | 0.0477 | 0.029 | 0.019 | 0.0446 |
Cytoplasmic Foci | 0.0087 | 0.0065 | 0.0099 | 0.0025 | 0.0013 | 0.003 | 0.0033 | 0.0043 | 0.0038 | 0.0001 | 0.0009 | 0.002 | 0.0001 | 0.0022 | 0.0023 | 0.0213 | 0.0016 | 0.0022 |
Eisosomes | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0 | 0 | 0.0012 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0005 | 0 | 0 |
Endoplasmic Reticulum | 0.8285 | 0.7797 | 0.8774 | 0.8525 | 0.8291 | 0.8817 | 0.8472 | 0.8586 | 0.9211 | 0.9797 | 0.9273 | 0.9257 | 0.9714 | 0.8964 | 0.9106 | 0.8727 | 0.9576 | 0.9287 |
Endosome | 0.0256 | 0.0316 | 0.0215 | 0.0158 | 0.0072 | 0.0112 | 0.0188 | 0.0222 | 0.0062 | 0.0021 | 0.0057 | 0.0166 | 0.0008 | 0.0066 | 0.009 | 0.0063 | 0.0042 | 0.0071 |
Golgi | 0.0037 | 0.006 | 0.0044 | 0.0054 | 0.0004 | 0.001 | 0.0083 | 0.0013 | 0.0029 | 0.0003 | 0.0013 | 0.0018 | 0.0001 | 0.0013 | 0.0026 | 0.0042 | 0.0008 | 0.001 |
Lipid Particles | 0.0058 | 0.0198 | 0.0126 | 0.0049 | 0.0012 | 0.003 | 0.0109 | 0.0176 | 0.0044 | 0.0008 | 0.0005 | 0.0021 | 0.0026 | 0.0034 | 0.0024 | 0.0215 | 0.0089 | 0.0028 |
Mitochondria | 0.0014 | 0.0055 | 0.0006 | 0.0077 | 0.0002 | 0.0019 | 0.0013 | 0.0037 | 0.0007 | 0.0002 | 0.0003 | 0.0013 | 0 | 0.0003 | 0.0005 | 0.0009 | 0.0001 | 0.0002 |
None | 0.0063 | 0.0094 | 0.0066 | 0.0016 | 0.0399 | 0.0027 | 0.0265 | 0.0117 | 0.0048 | 0.0001 | 0.0007 | 0.0015 | 0.0001 | 0.0027 | 0.0024 | 0.0008 | 0.0002 | 0.002 |
Nuclear Periphery | 0.0312 | 0.0169 | 0.0097 | 0.0167 | 0.0071 | 0.0134 | 0.0177 | 0.0108 | 0.0043 | 0.008 | 0.0048 | 0.0089 | 0.0151 | 0.0086 | 0.0055 | 0.0029 | 0.0041 | 0.0067 |
Nucleolus | 0.0002 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 |
Nucleus | 0.0138 | 0.0031 | 0.0009 | 0.0036 | 0.0029 | 0.0017 | 0.0009 | 0.0024 | 0.0004 | 0.0005 | 0.0005 | 0.0006 | 0.0002 | 0.0006 | 0.0003 | 0.0002 | 0.0003 | 0.0005 |
Peroxisomes | 0.0005 | 0.0001 | 0.0002 | 0.0006 | 0 | 0.0035 | 0.0005 | 0 | 0.0028 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0065 | 0 | 0 |
Punctate Nuclear | 0.0045 | 0.0005 | 0.0004 | 0.0022 | 0.0003 | 0.0008 | 0.0004 | 0.0004 | 0.0009 | 0 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.002 | 0.0001 | 0.0002 |
Vacuole | 0.0035 | 0.0107 | 0.0035 | 0.0036 | 0.0027 | 0.0031 | 0.0058 | 0.0053 | 0.0019 | 0.0023 | 0.0008 | 0.0023 | 0.0013 | 0.005 | 0.002 | 0.0014 | 0.0015 | 0.002 |
Vacuole Periphery | 0.0012 | 0.0011 | 0.0006 | 0.0011 | 0.0003 | 0.0006 | 0.0014 | 0.0006 | 0.0002 | 0.0005 | 0.0002 | 0.0008 | 0.0002 | 0.0005 | 0.0003 | 0.0003 | 0.0001 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 46.9746 | 35.7383 | 34.4751 | 44.6868 | 38.4434 | 32.631 | 34.6293 | 42.4769 | 37.3433 | 50.94 |
Translational Efficiency | 0.831 | 0.7234 | 0.8698 | 0.6533 | 0.6877 | 0.9661 | 0.8647 | 0.6158 | 0.8376 | 0.6293 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
133 | 383 | 1551 | 828 | 187 | 1463 | 247 | 1507 | 320 | 1846 | 1798 | 2335 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1255.73 | 950.99 | 1256.53 | 1271.45 | 931.35 | 935.92 | 1427.33 | 1203.94 | 1066.17 | 939.05 | 1279.99 | 1227.88 |
Standard Deviation | 174.29 | 151.65 | 207.34 | 221.26 | 135.41 | 135.37 | 185.67 | 187.10 | 221.13 | 139.04 | 212.79 | 202.47 |
Intensity Change Log 2 | — | -0.401024 | 0.000919 | 0.017948 | — | 0.007062 | 0.615924 | 0.370368 | — | -0.212980 | 0.295303 | 0.178650 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001310 | 0.000621 | 0.000407 | 0.002033 | 0.003809 | 0.000455 | 0.000069 | 0.002230 | 0.002770 | 0.000489 | 0.000360 | 0.002161 |
Bud Neck | 0.005243 | 0.001969 | 0.000668 | 0.001699 | 0.000410 | 0.000603 | 0.000370 | 0.001821 | 0.002419 | 0.000886 | 0.000627 | 0.001777 |
Bud Site | 0.020639 | 0.002456 | 0.001547 | 0.009884 | 0.007666 | 0.001133 | 0.000111 | 0.008279 | 0.013058 | 0.001408 | 0.001349 | 0.008848 |
Cell Periphery | 0.004976 | 0.000617 | 0.000326 | 0.002994 | 0.001129 | 0.000877 | 0.000463 | 0.001868 | 0.002728 | 0.000823 | 0.000345 | 0.002267 |
Cytoplasm | 0.033575 | 0.047238 | 0.023063 | 0.016265 | 0.024520 | 0.056394 | 0.001637 | 0.028463 | 0.028284 | 0.054494 | 0.020120 | 0.024137 |
Cytoplasmic Foci | 0.033588 | 0.019431 | 0.001339 | 0.004334 | 0.006465 | 0.010510 | 0.000034 | 0.008540 | 0.017738 | 0.012361 | 0.001159 | 0.007049 |
Eisosomes | 0.000410 | 0.000125 | 0.000008 | 0.000143 | 0.000322 | 0.000390 | 0.000026 | 0.000178 | 0.000359 | 0.000335 | 0.000011 | 0.000165 |
Endoplasmic Reticulum | 0.593029* | 0.832166* | 0.826401* | 0.894616* | 0.899842* | 0.860974* | 0.981828* | 0.822129* | 0.772323* | 0.854997* | 0.847753* | 0.847833* |
Endosome | 0.025316 | 0.010522 | 0.010447 | 0.011364 | 0.003470 | 0.004200 | 0.003290 | 0.020019 | 0.012550 | 0.005512 | 0.009464 | 0.016950 |
Golgi | 0.016151 | 0.009401 | 0.000727 | 0.003507 | 0.007934 | 0.010080 | 0.000180 | 0.010114 | 0.011349 | 0.009939 | 0.000652 | 0.007771 |
Lipid Particles | 0.048105 | 0.021529 | 0.002838 | 0.015301 | 0.013844 | 0.021549 | 0.000766 | 0.018396 | 0.028084 | 0.021545 | 0.002553 | 0.017298 |
Mitochondria | 0.000536 | 0.004885 | 0.000301 | 0.000708 | 0.001932 | 0.003433 | 0.000028 | 0.005170 | 0.001352 | 0.003734 | 0.000264 | 0.003588 |
Mitotic Spindle | 0.012272 | 0.000509 | 0.001118 | 0.001531 | 0.000104 | 0.000624 | 0.000009 | 0.007743 | 0.005161 | 0.000600 | 0.000966 | 0.005540 |
None | 0.000027 | 0.003766 | 0.000513 | 0.002214 | 0.000303 | 0.002809 | 0.000117 | 0.001426 | 0.000189 | 0.003008 | 0.000459 | 0.001706 |
Nuclear Periphery | 0.005403 | 0.006568 | 0.049509 | 0.001635 | 0.007557 | 0.004114 | 0.002607 | 0.006205 | 0.006662 | 0.004623 | 0.043066 | 0.004584 |
Nuclear Periphery Foci | 0.072402 | 0.015914 | 0.072223 | 0.020902 | 0.009337 | 0.011775 | 0.007321 | 0.037210 | 0.035548 | 0.012634 | 0.063307 | 0.031427 |
Nucleolus | 0.000055 | 0.000397 | 0.000012 | 0.000015 | 0.000055 | 0.000056 | 0.000000 | 0.000061 | 0.000055 | 0.000127 | 0.000010 | 0.000045 |
Nucleus | 0.006566 | 0.005805 | 0.001970 | 0.001154 | 0.005271 | 0.003018 | 0.000036 | 0.002253 | 0.005809 | 0.003596 | 0.001704 | 0.001863 |
Peroxisomes | 0.001081 | 0.001468 | 0.000025 | 0.000189 | 0.003119 | 0.002347 | 0.000004 | 0.000496 | 0.002272 | 0.002164 | 0.000022 | 0.000387 |
Vacuole | 0.113005 | 0.012826 | 0.006234 | 0.008377 | 0.002227 | 0.003278 | 0.001054 | 0.014293 | 0.048269 | 0.005259 | 0.005522 | 0.012195 |
Vacuole Periphery | 0.006311 | 0.001788 | 0.000323 | 0.001136 | 0.000682 | 0.001380 | 0.000049 | 0.003107 | 0.003021 | 0.001465 | 0.000286 | 0.002408 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.85 | 2.53 | -1.11 | -2.53 | -2.98 | 1.24 | 1.38 | 0.55 | -4.65 | -5.72 | 1.46 | 1.53 | 0.35 | -5.33 | -5.42 |
Bud Neck | 2.15 | 3.20 | 2.38 | 0.03 | -3.36 | -0.76 | 0.12 | -4.34 | -3.92 | -4.09 | 2.47 | 2.86 | 0.94 | -3.42 | -4.85 |
Bud Site | 2.74 | 2.89 | 1.46 | -4.00 | -4.63 | 1.35 | 1.57 | -0.21 | -6.75 | -7.86 | 2.96 | 2.96 | 0.92 | -7.89 | -7.78 |
Cell Periphery | 1.34 | 1.43 | 0.54 | -5.48 | -6.34 | 0.77 | 2.08 | -1.94 | -3.66 | -5.23 | 1.39 | 1.76 | 0.24 | -6.14 | -8.50 |
Cytoplasm | -1.04 | 1.99 | 2.88 | 5.13 | 2.24 | -5.78 | 5.10 | -0.95 | 6.99 | -12.00 | -5.07 | 2.27 | 1.22 | 9.26 | -1.91 |
Cytoplasmic Foci | 1.89 | 4.66 | 4.20 | 3.92 | -3.15 | -1.67 | 3.29 | -0.97 | 1.17 | -9.42 | 1.68 | 5.19 | 3.31 | 3.63 | -8.21 |
Eisosomes | 1.70 | 2.43 | 1.57 | -0.56 | -4.75 | -0.33 | 1.60 | 0.76 | 2.41 | -4.48 | 0.17 | 2.72 | 1.46 | 2.44 | -6.97 |
Endoplasmic Reticulum | -7.02 | -7.22 | -8.91 | -3.66 | -6.54 | 2.29 | -5.13 | 4.77 | 4.50 | 19.24 | -4.66 | -4.27 | -4.06 | 1.55 | 0.52 |
Endosome | 2.72 | 2.82 | 2.63 | -0.50 | -0.80 | -1.01 | 0.19 | -11.91 | -11.93 | -11.82 | 3.03 | 1.32 | -1.78 | -11.44 | -7.19 |
Golgi | 0.77 | 2.01 | 1.61 | 2.93 | -3.24 | -0.80 | 3.15 | -0.76 | -0.00 | -7.08 | 0.28 | 3.11 | 0.92 | 1.69 | -7.35 |
Lipid Particles | 2.67 | 4.62 | 3.25 | 1.35 | -5.82 | -1.88 | 3.50 | -1.29 | 0.98 | -10.03 | 1.45 | 5.39 | 2.16 | 1.66 | -10.63 |
Mitochondria | -2.22 | 0.62 | -0.40 | 2.11 | -1.07 | -1.33 | 1.86 | -2.11 | -1.43 | -4.44 | -2.90 | 1.74 | -2.32 | 0.12 | -4.36 |
Mitotic Spindle | 1.92 | 1.81 | 1.72 | -1.68 | -0.69 | -1.45 | 1.92 | -5.24 | -4.79 | -5.31 | 1.79 | 1.63 | 0.05 | -4.99 | -4.28 |
None | -1.41 | -4.42 | -1.64 | 0.52 | -1.28 | -4.07 | 1.16 | -2.42 | 1.85 | -2.95 | -3.86 | -2.04 | -2.72 | 1.42 | -2.25 |
Nuclear Periphery | -0.47 | -11.82 | 1.49 | 2.79 | 16.23 | 0.96 | 1.32 | 0.35 | -2.03 | -2.54 | 0.83 | -10.63 | 0.82 | -0.01 | 14.64 |
Nuclear Periphery Foci | 4.70 | 0.27 | 4.21 | -1.56 | 11.41 | -0.87 | 0.60 | -7.51 | -7.98 | -8.41 | 4.18 | -4.11 | 0.80 | -7.84 | 8.49 |
Nucleolus | -1.07 | 2.39 | 2.19 | 1.24 | -0.97 | 0.03 | 1.82 | -0.21 | -0.39 | -4.91 | -0.98 | 2.45 | 0.62 | 1.22 | -4.22 |
Nucleus | 0.52 | 2.27 | 2.58 | 2.79 | 1.52 | 0.70 | 1.57 | 0.88 | 1.34 | -6.90 | 1.15 | 1.98 | 1.88 | 3.27 | -0.67 |
Peroxisomes | -0.77 | 2.58 | 2.17 | 3.98 | -4.90 | 0.54 | 2.24 | 1.87 | 6.07 | -4.83 | 0.12 | 2.70 | 2.25 | 7.23 | -5.42 |
Vacuole | 6.54 | 6.98 | 6.83 | 1.22 | -1.80 | -1.08 | 1.28 | -7.15 | -7.23 | -8.79 | 6.12 | 6.07 | 5.13 | -5.86 | -5.84 |
Vacuole Periphery | 1.22 | 1.65 | 1.41 | 1.32 | -2.39 | -1.56 | 3.10 | -4.06 | -2.51 | -5.45 | 0.97 | 1.81 | 0.32 | -1.88 | -5.53 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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