Standard name
Human Ortholog
Description Putative serine/threonine protein phosphatase; putative phosphatase of the type 2A-like phosphatase family, required for glycogen accumulation; interacts with Tap42p, which binds to and regulates other protein phosphatases
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.97 0.96 0.97 0.99 0.91 0.87 0.88 0.78 0.84 0.7 0.75 0.97 0.99 0.99 0.89 0.9 0.85 0.77 0.79 0.56 0.78 0.76 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 0 0.07 0.19 0.13 0.28 0.21 0.44 0.27 0 0 0 0 0 0 0.1 0.05 0.23 0.07 0.06 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.08 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 0 1 2 2 0 0 0 0 0 0 3 2 1 5 3 3
Bud 0 1 0 0 0 0 3 4 7 12 12 10 0 1 0 0 0 1 2 5 0 4 6 9
Bud Neck 0 0 6 3 6 5 4 9 8 9 5 13 1 1 3 1 0 1 1 1 1 0 5 3
Bud Site 0 0 0 0 0 0 1 1 0 1 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 2 0 4 7 4 2 1 4 2 1 1 1 1 2 6 6 0 0 3 3 3 3
Cytoplasm 216 182 130 198 286 288 357 327 201 324 210 227 276 405 544 252 204 186 172 155 79 195 236 314
Endoplasmic Reticulum 0 2 1 0 0 2 0 1 1 3 1 2 0 0 0 16 10 13 5 0 1 2 1 0
Endosome 0 0 0 0 0 1 2 0 0 0 1 0 1 0 0 3 5 6 6 3 2 1 3 4
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 2 0 0 1 2 2 1 1 0 0 2 3
Mitochondria 13 1 0 1 1 22 79 49 73 83 133 83 0 1 0 7 10 7 21 9 32 17 19 22
Nucleus 0 0 2 0 2 3 8 5 0 2 3 1 3 0 1 0 1 3 0 0 0 2 2 0
Nuclear Periphery 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0
SpindlePole 0 1 1 1 0 4 7 6 6 11 7 18 0 1 1 3 0 3 3 3 2 5 10 9
Vac/Vac Membrane 0 4 2 3 3 12 7 6 3 7 6 5 4 4 4 31 18 16 3 8 5 2 5 6
Unique Cell Count 223 188 135 205 289 316 409 371 258 388 300 302 285 409 548 284 227 218 225 197 141 251 310 388
Labelled Cell Count 231 192 144 209 303 345 472 411 300 457 382 364 288 414 554 318 258 244 225 197 141 251 310 388


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 5.4 4.3 4.5 4.8 3.9 3.6 4.0 3.3 3.6 3.2 3.6 4.7 4.9 5.1 6.2 6.4 5.9 4.5 4.6 5.3
Std Deviation (1e-4) 1.2 1.8 1.1 1.9 1.8 1.7 1.6 1.6 1.4 1.5 1.4 1.6 1.7 1.5 1.8 2.2 2.5 2.4 1.6 1.7 2.1
Intensity Change (Log2) – – – 0.05 0.13 -0.15 -0.29 -0.11 -0.4 -0.28 -0.44 -0.28 0.12 0.16 0.22 0.5 0.56 0.45 0.06 0.1 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 1.9 -1.9 -3.0 -2.7 -4.7 -3.8 -6.1 -5.3 0.3 2.2 2.7 -2.6 -2.2 -3.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.4 0 2.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 3.4 2.6 2.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1197 0.3834 0.1793 0.2844 -0.1522 0.2375 -1.168 -0.9055 -0.8147 -0.6616 -0.8735 -0.9037 -0.6331 -0.6572 -0.4292 -0.6722 -0.5921 -0.6233
Actin 0.005 0.0001 0.0004 0.0008 0.0006 0.0005 0.0056 0.0001 0.0086 0.0002 0.0009 0.0029 0.0235 0.0018 0.016 0.0029 0.0032 0.0043
Bud 0.0011 0.0001 0.0002 0 0.0002 0.0002 0.0029 0.0105 0.0017 0.0001 0.0081 0.0014 0.0017 0.0134 0.0092 0.0034 0.0003 0.0031
Bud Neck 0.0005 0.0003 0.0002 0.0004 0.0002 0.0033 0.0015 0.0002 0.0019 0.0001 0.0002 0.0009 0.001 0.0003 0.0054 0.0004 0.0003 0.001
Bud Periphery 0.0005 0.0001 0.0004 0.0001 0.0003 0.0007 0.0016 0.0013 0.0034 0.0001 0.0011 0.0024 0.0013 0.009 0.0078 0.0063 0.0005 0.0017
Bud Site 0.0038 0.0189 0.0006 0.0001 0.0001 0.0001 0.0088 0.0184 0.0043 0.0003 0.0009 0.0003 0.0088 0.0136 0.0196 0.0006 0.0007 0.0002
Cell Periphery 0.0001 0.0002 0.0001 0.0001 0 0 0.0003 0.0002 0.0002 0.0001 0.0001 0.0001 0.0004 0.0007 0.0012 0.0002 0.0001 0.0001
Cytoplasm 0.1051 0.0909 0.1075 0.0974 0.1049 0.1389 0.1172 0.0966 0.1174 0.1174 0.0879 0.1011 0.1975 0.2364 0.2961 0.2622 0.2077 0.3037
Cytoplasmic Foci 0.0089 0.0012 0.0052 0.0021 0.0063 0.0063 0.0198 0.0022 0.0117 0.0071 0.01 0.0123 0.0274 0.0091 0.0239 0.0088 0.0147 0.0152
Eisosomes 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0005 0.0001 0.0001 0.0001 0.0004 0.0003 0.0005 0.0001 0.0002 0.0001
Endoplasmic Reticulum 0.0024 0.0007 0.0009 0.0008 0.0016 0.0005 0.0019 0.0006 0.002 0.0084 0.0009 0.0006 0.0056 0.0034 0.003 0.0051 0.0021 0.0032
Endosome 0.0022 0.0002 0.0022 0.0004 0.0011 0.0014 0.0091 0.0016 0.0148 0.0357 0.0244 0.001 0.0317 0.0018 0.0111 0.0044 0.023 0.0095
Golgi 0.0007 0 0.0007 0.0001 0.0001 0.0001 0.0022 0 0.0076 0.0015 0.0055 0.0004 0.0076 0.0005 0.0058 0.0012 0.0187 0.0027
Lipid Particles 0.0017 0.0001 0.0012 0 0.0003 0.0001 0.0157 0 0.009 0.0026 0.0453 0.0006 0.0127 0.0005 0.0046 0.0101 0.0133 0.0021
Mitochondria 0.0026 0.0001 0.0008 0.0002 0.0003 0.0007 0.003 0.0003 0.0091 0.0019 0.006 0.0004 0.0054 0.0008 0.0096 0.0014 0.0168 0.0028
None 0.8533 0.8739 0.8642 0.8957 0.8752 0.8371 0.7575 0.8557 0.7862 0.8092 0.7776 0.8626 0.626 0.6723 0.5528 0.6515 0.6338 0.6158
Nuclear Periphery 0.001 0.0017 0.001 0.0002 0.0015 0.0005 0.0107 0.0009 0.0019 0.0043 0.0035 0.0005 0.0136 0.0032 0.0024 0.0103 0.0038 0.0065
Nucleolus 0.0006 0.0001 0.0003 0.0001 0.0003 0.0001 0.0024 0.0003 0.001 0.0009 0.0009 0.0003 0.0059 0.0005 0.0009 0.0006 0.0004 0.0006
Nucleus 0.0041 0.0105 0.0106 0.0009 0.002 0.0062 0.0115 0.0092 0.0049 0.0032 0.0038 0.0054 0.0103 0.0279 0.0102 0.011 0.037 0.0135
Peroxisomes 0.0034 0 0.0004 0.0001 0.0006 0.0006 0.0096 0 0.0076 0.0004 0.0172 0.0026 0.0028 0.0012 0.0116 0.007 0.0142 0.0047
Punctate Nuclear 0.0019 0.0003 0.0022 0.0003 0.0041 0.0022 0.0145 0.0007 0.0037 0.002 0.0028 0.0033 0.006 0.002 0.0036 0.0064 0.0065 0.0077
Vacuole 0.0006 0.0003 0.0005 0.0001 0.0002 0.0003 0.0022 0.0008 0.0022 0.0034 0.0015 0.0006 0.0073 0.0009 0.0037 0.0043 0.0014 0.0011
Vacuole Periphery 0.0002 0 0.0001 0 0.0001 0.0001 0.0015 0.0002 0.0004 0.0011 0.0011 0.0001 0.0031 0.0002 0.0009 0.0016 0.0012 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.9578 16.0386 17.1909 17.686 16.8003 14.943 18.9194 17.9379 20.79 20.72
Translational Efficiency 1.1753 0.6433 0.593 0.8143 0.7141 1.011 0.7154 0.6467 0.6314 0.7167

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative serine/threonine protein phosphatase; putative phosphatase of the type 2A-like phosphatase family, required for glycogen accumulation; interacts with Tap42p, which binds to and regulates other protein phosphatases
Localization
Cell Percentages cytoplasm (10%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ppg1

Ppg1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ppg1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available