Standard name
Human Ortholog
Description Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.98 0.95 0.96 0.9 0.89 0.81 0.77 0.71 0.78 0.99 0.99 0.99 0.92 0.96 0.96 0.98 0.96 0.96 0.92 0.9 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.1 0.12 0.24 0.23 0.3 0.18 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0 0.08 0 0.05 0.12 0.09 0.09 0 0 0 0.07 0.07 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 1 0 1 1 1
Bud 0 0 1 0 0 0 2 4 10 7 5 1 0 0 0 0 0 0 0 0 1 5 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 3 2 8 5 6 10 5 9 7 13 7 0 2 2 18 11 15 0 0 0 0 0 0
Cytoplasm 267 587 445 330 366 442 499 451 387 355 337 333 429 471 532 354 361 259 584 442 268 329 368
Endoplasmic Reticulum 0 1 0 1 0 0 1 1 0 5 0 2 1 2 16 10 8 0 0 2 3 4 3
Endosome 0 0 0 0 0 1 0 0 1 1 4 0 0 0 3 0 0 1 3 1 2 2 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 1 0 3
Mitochondria 1 2 3 7 9 49 66 132 113 151 78 0 0 3 3 1 3 0 3 1 1 3 1
Nucleus 1 1 0 1 0 2 4 2 7 3 8 1 2 3 16 3 13 0 3 0 1 0 0
Nuclear Periphery 0 0 0 1 0 0 1 0 2 5 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 1 0 1 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 2 0 0
Vac/Vac Membrane 0 1 11 17 14 37 18 27 60 47 39 1 1 1 40 25 16 0 6 6 3 12 11
Unique Cell Count 269 593 455 347 380 493 562 559 500 497 431 337 434 477 576 368 377 264 611 462 290 366 402
Labelled Cell Count 272 595 468 362 396 542 596 627 589 588 479 338 436 482 631 404 416 264 611 462 290 366 402


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 7.3 7.3 7.0 7.2 6.0 6.0 5.5 5.4 5.5 5.8 6.7 6.9 6.7 9.4 10.3 9.3 6.5 6.4 6.9
Std Deviation (1e-4) 0.9 1.0 0.9 1.4 1.2 1.2 1.0 1.0 1.0 1.0 1.1 0.9 1.0 0.9 1.7 1.9 2.2 1.3 1.0 1.3
Intensity Change (Log2) -0.05 -0.0 -0.26 -0.26 -0.4 -0.43 -0.41 -0.32 -0.11 -0.08 -0.11 0.38 0.5 0.36 -0.17 -0.19 -0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 2.6 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.4 -0.2 0.3 -1.2 -0.2 -0.4 0.9 -0.2 0 0 0 1.4 1.2 1.9
Cytoplasm -2.1 -1.3 -5.1 -5.5 -8.5 -9.4 -11.1 -9.1 1.1 1.2 1.1 -3.9 -1.4 -1.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 3.6 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.1 6.3 7.0 10.7 10.4 12.4 9.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 3.6 0 4.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.9 1.1 3.6 0.7 2.0 5.6 4.5 4.3 -2.4 -2.8 -3.0 3.3 3.1 1.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3492 1.4491 1.1635 1.0573 1.4351 1.5971 3.8048 3.7989 3.773 3.6053 3.4895 3.6957 2.4237 2.9285 2.9214 2.9396 2.7538 2.8657
Actin 0.0155 0.0006 0.0322 0.0005 0.0005 0.0092 0.0346 0.0018 0.008 0.0016 0.0517 0.02 0.0004 0.0014 0.0011 0.0009 0.0227 0.0007
Bud 0.0015 0.0009 0.0012 0.0004 0.0039 0.0049 0.0028 0.0016 0.0031 0.0004 0.0014 0.0018 0.0032 0.0109 0.0078 0.0045 0.0012 0.0032
Bud Neck 0.0054 0.0002 0.0033 0.0008 0.0025 0.0073 0.0237 0.001 0.0019 0.0009 0.0008 0.0029 0.0003 0.0006 0.0007 0.0003 0.0006 0.0023
Bud Periphery 0.0018 0.0002 0.0013 0.0002 0.0018 0.0027 0.0059 0.0008 0.0025 0.0002 0.0006 0.0035 0.0018 0.0025 0.0036 0.0018 0.0042 0.0008
Bud Site 0.0124 0.0025 0.0095 0.0008 0.0114 0.0007 0.0261 0.0221 0.0068 0.0009 0.0008 0.0014 0.0016 0.0127 0.0041 0.0003 0.0032 0.0003
Cell Periphery 0.0005 0.0001 0.0003 0 0.0002 0.0003 0.0009 0.0003 0.0004 0 0.0001 0.0002 0.0001 0.0002 0.0002 0 0.0004 0.0001
Cytoplasm 0.4881 0.7327 0.6711 0.7388 0.6022 0.692 0.5613 0.8452 0.8541 0.8459 0.5477 0.8105 0.7493 0.8714 0.7859 0.7672 0.7899 0.8166
Cytoplasmic Foci 0.0291 0.0085 0.0209 0.0152 0.0208 0.0223 0.0383 0.0095 0.0142 0.039 0.0525 0.0145 0.0146 0.0125 0.0096 0.0321 0.0227 0.0152
Eisosomes 0.0003 0 0.0006 0 0 0.0001 0.0003 0 0 0 0.0001 0.0001 0 0 0 0 0.0003 0
Endoplasmic Reticulum 0.0075 0.0031 0.0028 0.0055 0.0097 0.0062 0.0086 0.0026 0.0029 0.0026 0.0027 0.0029 0.0025 0.0018 0.0021 0.0021 0.0033 0.0023
Endosome 0.0599 0.0045 0.0079 0.0137 0.0245 0.0217 0.0523 0.0032 0.0129 0.0215 0.0662 0.0117 0.004 0.0054 0.0025 0.0044 0.0118 0.0059
Golgi 0.0127 0.0003 0.0019 0.0004 0.0008 0.0058 0.0128 0.0005 0.0042 0.004 0.0185 0.0082 0.0004 0.0005 0.0005 0.0003 0.0052 0.0002
Lipid Particles 0.009 0.0002 0.0079 0.0003 0.0003 0.0043 0.0223 0.0003 0.0068 0.0035 0.0292 0.0119 0.0005 0.0001 0.0001 0.0001 0.0124 0.0001
Mitochondria 0.0082 0.0003 0.0109 0.0003 0.002 0.0069 0.0053 0.0004 0.0028 0.0007 0.0035 0.0113 0.0005 0.0004 0.0006 0.0007 0.007 0.0004
None 0.232 0.2318 0.1944 0.2084 0.2877 0.1904 0.1076 0.0921 0.0552 0.0449 0.2018 0.0673 0.1992 0.0666 0.1602 0.1574 0.0483 0.1283
Nuclear Periphery 0.0396 0.0015 0.0023 0.0027 0.0052 0.0052 0.0272 0.002 0.0019 0.0017 0.002 0.0042 0.0013 0.0012 0.0013 0.002 0.0022 0.0018
Nucleolus 0.0049 0.0004 0.0021 0.0002 0.0006 0.0004 0.0038 0.0005 0.0007 0.0002 0.0003 0.001 0.0004 0.0002 0.0003 0.0002 0.0011 0.0003
Nucleus 0.0275 0.0067 0.006 0.0049 0.0146 0.0072 0.0208 0.011 0.0057 0.0065 0.0019 0.0106 0.0083 0.0066 0.0066 0.0078 0.006 0.0116
Peroxisomes 0.0044 0.0002 0.0083 0.0002 0.0004 0.0029 0.0172 0.0005 0.0035 0.0131 0.0126 0.0077 0.0006 0.0004 0.0076 0.0109 0.045 0.0002
Punctate Nuclear 0.0201 0.0034 0.0131 0.0039 0.0024 0.0054 0.0179 0.0027 0.0026 0.0113 0.0027 0.0056 0.0098 0.003 0.0037 0.0056 0.0067 0.0084
Vacuole 0.0142 0.0017 0.0018 0.0021 0.007 0.0035 0.0076 0.0017 0.0087 0.0008 0.0024 0.0021 0.0009 0.0014 0.0011 0.0012 0.0049 0.001
Vacuole Periphery 0.0053 0.0002 0.0004 0.0005 0.0014 0.0009 0.0027 0.0003 0.0011 0.0002 0.0007 0.0007 0.0001 0.0002 0.0002 0.0003 0.0008 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.2386 19.1934 15.1712 22.728 22.3911 21.9253 30.5863 27.7622 28.2401 26.2294
Translational Efficiency 0.9409 0.8576 0.8139 0.6841 0.6796 1.1616 0.7663 0.6544 0.6927 0.7536

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2136 1001 1410 1217 1485 1778 1070 375 3621 2779 2480 1592

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 753.43 913.68 1062.08 1085.29 811.96 904.00 1015.65 1381.15 777.43 907.49 1042.05 1154.98
Standard Deviation 87.60 133.27 131.48 167.06 97.99 118.16 133.16 216.70 96.40 123.91 134.19 219.45
Intensity Change Log 2 0.278215 0.495347 0.526535 0.154914 0.322923 0.766389 0.215576 0.408486 0.655908

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000063 0.000169 0.000333 0.007763 0.000208 0.000894 0.000221 0.000063 0.000123 0.000633 0.000285 0.005949
Bud Neck 0.004531 0.003355 0.003760 0.011388 0.003108 0.006456 0.003467 0.003387 0.003947 0.005339 0.003633 0.009504
Bud Site 0.004539 0.008620 0.007661 0.043877 0.005120 0.014239 0.006374 0.011387 0.004777 0.012215 0.007106 0.036224
Cell Periphery 0.000090 0.000071 0.000108 0.000195 0.000099 0.000065 0.000099 0.000029 0.000094 0.000067 0.000104 0.000156
Cytoplasm 0.701301* 0.711000* 0.444199* 0.512937* 0.690414* 0.708167* 0.631488* 0.806404* 0.696836* 0.709187* 0.525005* 0.582064*
Cytoplasmic Foci 0.089910 0.091851 0.004694 0.017766 0.123564 0.104920 0.011130 0.001782 0.103712 0.100212 0.007471 0.014001
Eisosomes 0.000046 0.000029 0.000019 0.000030 0.000045 0.000034 0.000026 0.000002 0.000046 0.000032 0.000022 0.000024
Endoplasmic Reticulum 0.000501 0.001031 0.002967 0.002141 0.001608 0.000642 0.001868 0.000046 0.000955 0.000782 0.002493 0.001647
Endosome 0.003272 0.010683 0.001467 0.010879 0.005098 0.008696 0.002152 0.000208 0.004021 0.009412 0.001762 0.008366
Golgi 0.000790 0.001639 0.000076 0.009096 0.000819 0.004296 0.000086 0.001127 0.000802 0.003339 0.000080 0.007219
Lipid Particles 0.001565 0.001461 0.000791 0.004579 0.001813 0.001006 0.000906 0.000055 0.001667 0.001170 0.000841 0.003513
Mitochondria 0.001532 0.001458 0.001019 0.010799 0.001051 0.003108 0.000283 0.001109 0.001334 0.002514 0.000701 0.008516
Mitotic Spindle 0.002580 0.007749 0.008386 0.099856 0.001516 0.012073 0.006222 0.010956 0.002144 0.010515 0.007452 0.078915
None 0.007957 0.003574 0.006008 0.010801 0.002292 0.001695 0.005068 0.012176 0.005634 0.002372 0.005603 0.011125
Nuclear Periphery 0.000175 0.000341 0.002112 0.001009 0.000290 0.000167 0.001142 0.000170 0.000222 0.000230 0.001694 0.000811
Nuclear Periphery Foci 0.000530 0.001539 0.001400 0.002302 0.001262 0.000661 0.002457 0.000031 0.000830 0.000977 0.001856 0.001767
Nucleolus 0.000433 0.001048 0.000227 0.000320 0.000375 0.000393 0.000205 0.000154 0.000409 0.000629 0.000218 0.000281
Nucleus 0.132474* 0.060078 0.448250* 0.152577* 0.103062 0.048725 0.210307* 0.138285* 0.120412 0.052814 0.345589* 0.149211*
Peroxisomes 0.000813 0.001322 0.000115 0.002958 0.000852 0.004331 0.000158 0.000174 0.000829 0.003247 0.000134 0.002302
Vacuole 0.046265 0.090301 0.066266 0.090827 0.056716 0.077478 0.116214 0.009998 0.050551 0.082097 0.087816 0.071788
Vacuole Periphery 0.000635 0.002681 0.000142 0.007900 0.000688 0.001954 0.000124 0.002459 0.000657 0.002216 0.000134 0.006618

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.75 -4.83 -2.73 -2.56 -1.93 -1.95 -1.84 2.80 2.50 5.65 -2.35 -5.21 -2.60 -1.58 -1.89
Bud Neck 1.91 -1.07 -1.49 -2.74 -0.71 -5.30 0.09 -0.16 3.59 -0.20 -2.85 -0.72 -1.91 0.17 -1.34
Bud Site -2.71 -2.09 -6.99 -4.06 -4.87 -6.41 -0.45 -0.87 2.77 -0.61 -7.32 -1.97 -6.76 -1.77 -5.18
Cell Periphery 3.47 -4.50 -3.88 -5.99 1.06 3.59 -0.68 7.63 9.23 15.20 5.29 -4.02 -0.80 -5.09 2.82
Cytoplasm 0.23 43.76 29.40 23.13 -8.94 -1.33 19.66 -3.17 -2.22 -17.57 -0.54 44.18 23.62 22.08 -11.28
Cytoplasmic Foci -0.11 31.90 28.94 21.02 -7.37 3.74 29.21 31.30 31.30 10.89 1.24 42.81 41.20 35.78 -2.63
Eisosomes 6.34 6.33 8.52 1.57 2.54 3.03 1.98 12.26 13.32 19.14 5.81 5.63 12.13 6.20 8.93
Endoplasmic Reticulum -4.10 -20.04 -5.74 -1.32 13.47 2.56 -3.09 4.26 4.10 18.79 1.01 -13.70 -0.14 -1.51 17.03
Endosome -8.20 5.56 0.92 7.88 -1.93 -4.62 8.49 14.85 13.26 13.23 -9.51 9.78 4.37 11.12 -0.59
Golgi -1.54 6.26 -1.31 -0.17 -2.26 -4.47 7.27 0.47 3.94 -0.91 -4.72 9.15 -1.17 2.20 -2.40
Lipid Particles 0.49 1.28 -0.87 -1.14 -1.47 4.44 3.05 10.26 9.98 19.83 3.14 2.56 0.12 -1.10 -0.81
Mitochondria 0.24 1.03 -1.27 -1.38 -2.16 -2.33 1.29 0.52 3.18 -1.17 -1.85 1.66 -1.28 0.31 -2.56
Mitotic Spindle -2.37 -4.53 -9.12 -7.32 -7.10 -5.49 -4.30 -2.00 0.84 0.23 -5.57 -6.18 -9.40 -6.64 -6.88
None 5.17 3.29 -0.57 -6.09 -4.33 1.37 -4.62 -9.06 -10.28 -6.73 6.64 0.89 -5.15 -11.83 -6.96
Nuclear Periphery -3.05 -29.89 -16.68 -12.61 12.79 2.56 -19.65 -2.48 -6.09 18.22 -0.64 -35.22 -16.20 -16.02 14.72
Nuclear Periphery Foci -5.33 -8.61 -6.83 -0.39 1.37 2.53 -4.09 4.98 7.48 15.88 -0.95 -8.55 -2.66 -2.20 6.50
Nucleolus -2.03 4.74 0.25 2.04 -1.13 -0.37 1.94 1.83 2.25 -0.27 -2.00 3.80 0.59 2.13 -1.34
Nucleus 18.86 -44.01 -13.82 -24.79 26.24 15.57 -21.25 -10.44 -16.85 5.30 26.59 -44.67 -17.92 -31.23 21.64
Peroxisomes -2.04 5.12 0.65 2.32 -2.79 -6.15 8.09 6.95 7.58 0.46 -6.41 8.01 1.74 6.87 -2.72
Vacuole -10.83 -15.12 -24.24 -14.51 -11.36 -7.56 -19.41 4.14 9.17 19.78 -14.03 -24.22 -21.25 -13.77 -2.76
Vacuole Periphery -2.15 2.55 -1.44 0.91 -2.30 -2.40 3.41 -0.46 0.53 -0.98 -3.23 3.95 -1.48 0.89 -2.49
Standard name
Human Ortholog
Description Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (90%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Abz1

Abz1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Abz1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available