Standard name
Human Ortholog
Description Mevalonate pyrophosphate decarboxylase; essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.05 0.07 0.09 0.07 0.08 0.06 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.98 0.99 0.98 0.95 0.88 0.81 0.83 0.86 0.85 0.83 0.99 0.99 0.99 0.99 0.99 0.99 0.87 0.96 0.95 0.94 0.95 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0.08 0.12 0.11 0.09 0 0.06 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 3 8 11 4 7 7 10 0 0 0 0 0 0 2 0 0 0 0 0
Bud 0 0 1 1 0 2 7 3 2 5 5 3 0 0 0 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 3 12 10 16 33 68 82 29 52 25 49 0 7 3 11 9 6 0 0 0 0 0 0
Cytoplasm 45 119 320 435 690 610 908 767 359 596 331 482 80 210 215 440 287 264 56 116 314 49 119 202
Endoplasmic Reticulum 0 1 2 5 11 11 87 115 46 59 17 36 1 1 0 22 15 8 0 0 5 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 3 3 3 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1 2 0 0 0 2 9 6 8 11 12 20 0 0 0 0 0 1 0 0 1 0 0 0
Nucleus 0 0 2 3 3 2 7 10 5 6 2 3 0 1 3 0 0 2 0 2 1 0 1 3
Nuclear Periphery 0 0 0 0 0 0 1 0 0 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 4 2 0 4 12 21 11 10 11 21 1 0 1 1 2 0 0 0 2 1 0 2
Unique Cell Count 46 122 327 441 704 644 1031 945 433 693 391 580 81 212 218 445 289 267 65 122 330 53 125 214
Labelled Cell Count 46 125 341 456 720 670 1111 1018 466 749 410 625 82 219 222 475 313 281 65 122 330 53 125 214


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 23.3 26.6 25.9 24.2 23.7 22.4 22.4 22.3 20.2 19.5 18.4 18.4 30.6 32.4 29.8 49.1 47.2 48.9 20.8 30.3 31.9
Std Deviation (1e-4) 6.3 5.7 4.9 5.2 4.2 4.6 4.8 4.9 4.5 4.3 4.3 4.1 7.8 6.0 5.9 14.4 14.5 17.2 7.2 8.0 9.4
Intensity Change (Log2) – – – -0.1 -0.13 -0.21 -0.21 -0.21 -0.36 -0.41 -0.5 -0.49 0.24 0.32 0.2 0.92 0.87 0.92 -0.32 0.23 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.2 -1.3 1.0 2.0 3.0 1.8 2.4 1.6 2.8 0 -0.2 -1.6 -1.0 -0.4 -1.0
Cytoplasm 0.8 0.2 -2.3 -5.2 -7.4 -6.6 -5.8 -6.1 -6.7 0.5 1.1 0.7 1.1 1.5 1.0
Endoplasmic Reticulum 0 0 0 5.0 6.2 5.6 5.0 3.1 4.0 0 0 0 3.4 3.5 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 3.2 3.4 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 1.1 1.3 0 1.5 2.1 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 14.8916 22.0211 18.2987 18.0957 17.7956 20.3868 14.2551 25.4683 22.7005 17.5598 18.7638 21.6883 17.2935 23.533 22.1682 20.4433 21.4993 21.921
Actin 0.0287 0.0002 0.0029 0.0001 0.0222 0.0004 0.0997 0.0073 0.0221 0.036 0.0023 0.0018 0.0006 0.0003 0.0036 0 0 0.0003
Bud 0.0009 0.0001 0.0003 0.0001 0.0006 0.0002 0.001 0 0.0002 0.0022 0.0005 0.0006 0.0001 0.0001 0.0006 0 0 0.0001
Bud Neck 0.0025 0.0001 0.0007 0.0003 0.0012 0.0007 0.0011 0.0001 0.0005 0.001 0.0003 0.0009 0.0002 0.0001 0.0001 0.0002 0.0001 0.0004
Bud Periphery 0.0007 0 0.0003 0 0.001 0.0001 0.001 0 0.0002 0.0022 0.0002 0.0003 0 0 0.0001 0 0 0
Bud Site 0.0033 0.0001 0.0016 0 0.0031 0 0.0032 0.0004 0.0011 0.0104 0.0004 0.0001 0 0 0.0001 0 0 0
Cell Periphery 0.0003 0 0.0001 0 0.0001 0 0.0003 0 0.0001 0.0002 0 0 0 0 0 0 0 0
Cytoplasm 0.7958 0.9955 0.9319 0.99 0.9121 0.9719 0.7425 0.9894 0.9453 0.7917 0.9696 0.9674 0.983 0.9958 0.9903 0.9921 0.9754 0.992
Cytoplasmic Foci 0.0312 0.0007 0.007 0.0004 0.0038 0.0018 0.0194 0.0003 0.0033 0.0102 0.0065 0.0045 0.0006 0.0004 0.0005 0.0002 0.0003 0.0008
Eisosomes 0.0004 0 0 0 0.0002 0 0.0004 0 0.0001 0.0003 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0145 0.0003 0.0018 0.0003 0.0023 0.0015 0.0104 0.0001 0.0003 0.0008 0.0033 0.0054 0.0008 0.0003 0.0002 0.0002 0.0004 0.0004
Endosome 0.0267 0.0002 0.0069 0.0002 0.0128 0.0014 0.0209 0 0.0052 0.0233 0.005 0.0074 0.0003 0.0002 0.0002 0.0001 0.0001 0.0003
Golgi 0.0091 0 0.0025 0 0.0117 0.0001 0.0172 0.0001 0.0037 0.0171 0.0007 0.0012 0 0 0.0001 0 0 0
Lipid Particles 0.009 0 0.0041 0 0.004 0 0.009 0 0.0031 0.0168 0.0002 0.0001 0 0 0 0 0 0
Mitochondria 0.0039 0.0001 0.0133 0 0.0073 0.0014 0.013 0.0007 0.0079 0.0301 0.0018 0.0042 0.0001 0.0001 0.0005 0 0 0
None 0.0057 0.0003 0.0009 0.0003 0.0008 0.0006 0.018 0.0001 0.0004 0.0006 0.003 0.0005 0.0009 0.0002 0.0003 0.0001 0.0002 0.0003
Nuclear Periphery 0.0267 0.0003 0.0038 0.001 0.0078 0.0026 0.0101 0.0002 0.0006 0.0035 0.0011 0.001 0.0027 0.0005 0.0005 0.0011 0.0052 0.0008
Nucleolus 0.0021 0 0.0053 0 0.0002 0.0011 0.0012 0 0 0.0013 0 0 0 0 0 0 0 0
Nucleus 0.0193 0.0019 0.0096 0.0069 0.0043 0.0137 0.0157 0.0009 0.0012 0.0113 0.0034 0.0027 0.01 0.002 0.0026 0.0057 0.0176 0.0042
Peroxisomes 0.0061 0 0.0013 0 0.0011 0 0.005 0.0002 0.0031 0.0118 0.0002 0.0003 0 0 0.0001 0 0 0
Punctate Nuclear 0.0078 0.0001 0.0034 0.0001 0.0008 0.0015 0.0056 0 0.0011 0.0031 0.0004 0.0002 0.0003 0.0001 0.0001 0 0.0002 0.0002
Vacuole 0.0035 0.0002 0.0016 0.0002 0.0012 0.0006 0.0038 0 0.0003 0.0206 0.0006 0.0009 0.0004 0.0001 0.0002 0.0002 0.0003 0.0002
Vacuole Periphery 0.0016 0 0.0007 0.0001 0.0012 0.0002 0.0017 0 0.0002 0.0058 0.0003 0.0007 0.0001 0 0.0001 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 168.1511 132.5107 138.8892 143.8276 138.1363 145.3725 130.4225 138.7511 132.6106 134.0223
Translational Efficiency 1.3022 1.5022 1.4836 1.4086 1.5505 1.4918 1.4589 1.3928 1.536 1.5891

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1594 147 1686 664 1218 183 539 1188 2812 330 2225 1852

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1719.56 2164.87 2219.78 3254.28 1774.26 2015.89 2770.05 3254.22 1743.25 2082.25 2353.08 3254.24
Standard Deviation 270.90 308.03 494.37 774.30 287.75 312.97 519.66 770.06 279.64 319.48 553.35 771.58
Intensity Change Log 2 — 0.332241 0.368377 0.920299 — 0.184199 0.642695 0.875094 — 0.258960 0.514185 0.897520

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000106 0.000250 0.000124 0.000426 0.000113 0.000221 0.000176 0.000304 0.000109 0.000234 0.000137 0.000348
Bud Neck 0.000216 0.000765 0.000700 0.001865 0.000186 0.000690 0.001619 0.002300 0.000203 0.000723 0.000922 0.002144
Bud Site 0.002125 0.002892 0.002074 0.009807 0.001232 0.004912 0.003426 0.008512 0.001738 0.004012 0.002401 0.008976
Cell Periphery 0.000127 0.000373 0.000189 0.002552 0.000140 0.000209 0.001035 0.001236 0.000133 0.000282 0.000394 0.001708
Cytoplasm 0.952819* 0.877438* 0.857190* 0.857387* 0.938537* 0.902105* 0.859266* 0.854352* 0.946633* 0.891117* 0.857693* 0.855440*
Cytoplasmic Foci 0.007975 0.018574 0.004997 0.005983 0.005553 0.021281 0.010457 0.007077 0.006926 0.020075 0.006320 0.006685
Eisosomes 0.000001 0.000002 0.000001 0.000004 0.000001 0.000001 0.000002 0.000004 0.000001 0.000001 0.000001 0.000004
Endoplasmic Reticulum 0.000236 0.000183 0.000276 0.000046 0.000515 0.000091 0.000063 0.000302 0.000357 0.000132 0.000224 0.000210
Endosome 0.000249 0.000669 0.000322 0.000670 0.000255 0.000637 0.000381 0.004147 0.000251 0.000651 0.000337 0.002901
Golgi 0.001727 0.001894 0.000593 0.005316 0.001581 0.001929 0.001807 0.004319 0.001664 0.001914 0.000887 0.004676
Lipid Particles 0.000691 0.001465 0.000745 0.004254 0.000379 0.000858 0.003134 0.003266 0.000556 0.001128 0.001324 0.003620
Mitochondria 0.000341 0.000041 0.000080 0.000531 0.000040 0.000040 0.000075 0.001980 0.000211 0.000040 0.000079 0.001460
Mitotic Spindle 0.000580 0.000577 0.001728 0.001357 0.000526 0.005506 0.001059 0.005750 0.000557 0.003310 0.001566 0.004175
None 0.003437 0.007490 0.006523 0.002278 0.006271 0.006547 0.003738 0.002359 0.004664 0.006967 0.005848 0.002330
Nuclear Periphery 0.000118 0.000076 0.000137 0.000100 0.000034 0.000044 0.000147 0.000223 0.000082 0.000058 0.000139 0.000179
Nuclear Periphery Foci 0.002270 0.004534 0.001826 0.002388 0.003509 0.002779 0.003634 0.002520 0.002807 0.003561 0.002264 0.002473
Nucleolus 0.000045 0.000181 0.000093 0.000115 0.000033 0.000067 0.000093 0.000188 0.000040 0.000118 0.000093 0.000162
Nucleus 0.020955 0.064585 0.102827 0.054388 0.037296 0.041735 0.073299 0.051220 0.028033 0.051913 0.095674 0.052356
Peroxisomes 0.000042 0.000036 0.000019 0.000061 0.000010 0.000075 0.000030 0.000090 0.000028 0.000058 0.000022 0.000080
Vacuole 0.005207 0.013679 0.019367 0.045906 0.003384 0.009991 0.035548 0.046201 0.004417 0.011634 0.023287 0.046095
Vacuole Periphery 0.000733 0.004298 0.000190 0.004567 0.000406 0.000283 0.001011 0.003650 0.000592 0.002072 0.000389 0.003979

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.61 -2.71 -4.66 -0.95 -3.90 -2.47 -4.63 -3.22 0.19 0.20 -4.20 -4.83 -5.48 -0.27 -3.35
Bud Neck -3.71 -10.90 -6.72 -3.11 -3.27 -4.61 -9.99 -10.93 -6.40 0.84 -5.82 -14.57 -12.79 -6.76 -4.25
Bud Site -2.29 -1.49 -4.31 -3.13 -3.92 -3.91 -5.50 -7.29 -0.71 -1.50 -4.37 -4.58 -7.75 -2.72 -5.43
Cell Periphery -4.59 -5.63 -8.18 -6.79 -7.71 -2.59 -7.45 -7.49 -6.62 0.10 -5.26 -8.97 -11.03 -9.17 -8.20
Cytoplasm 6.85 30.16 19.24 3.56 0.50 5.91 17.60 22.53 7.22 -0.98 9.26 34.45 30.25 7.68 1.01
Cytoplasmic Foci -3.25 -0.47 -1.78 2.77 -1.47 -4.82 -7.45 -4.85 3.80 4.69 -5.68 -5.26 -4.03 4.72 1.23
Eisosomes -3.06 -6.66 -6.39 -3.24 -4.66 -4.07 -9.27 -7.93 -3.43 -0.61 -4.79 -11.36 -9.90 -4.54 -5.11
Endoplasmic Reticulum 0.28 -0.14 2.38 2.20 3.44 1.43 1.53 1.27 -0.71 -1.19 1.52 1.05 1.84 0.87 1.77
Endosome -2.36 -3.69 -1.10 1.50 0.37 -2.40 -2.77 -3.10 -2.09 -2.63 -3.37 -4.61 -3.23 -1.58 -2.59
Golgi -0.54 6.56 -0.77 -0.63 -1.49 -0.45 0.24 -0.85 -0.50 -0.90 -0.62 5.47 -1.09 -0.79 -2.29
Lipid Particles -2.54 -3.60 -8.37 -5.50 -7.38 -3.76 -7.82 -10.31 -7.46 0.42 -3.83 -8.68 -12.94 -8.73 -7.67
Mitochondria 1.04 0.86 0.14 -1.21 -0.96 0.27 -1.67 -1.87 -1.88 -1.81 1.05 0.76 -1.33 -2.12 -1.96
Mitotic Spindle -0.13 -4.43 -0.78 -0.75 2.67 -1.66 -1.66 -2.31 0.57 -1.39 -1.68 -4.64 -2.50 0.29 0.31
None -1.88 -2.87 3.67 2.72 5.49 0.32 4.12 6.14 2.30 2.99 -1.22 -0.30 7.07 3.51 5.99
Nuclear Periphery 0.14 -1.13 -0.34 -1.85 4.29 -3.27 -10.64 -5.77 -4.79 0.47 0.00 -2.66 -1.99 -4.86 1.31
Nuclear Periphery Foci -3.63 -0.53 -2.22 2.65 -2.39 0.38 -2.87 0.87 0.25 3.85 -2.97 -0.86 -1.24 2.34 -0.56
Nucleolus -1.11 -8.48 -3.06 0.53 1.10 -5.25 -11.69 -7.72 -4.81 -2.30 -1.54 -11.61 -7.79 -0.51 -0.82
Nucleus -5.70 -31.12 -13.27 0.03 11.75 -1.73 -10.94 -10.08 -4.07 4.65 -6.04 -31.11 -17.96 -2.39 14.15
Peroxisomes -0.35 1.08 -1.07 -0.61 -2.61 -3.65 -5.93 -6.73 1.10 -2.49 -3.14 -1.31 -4.54 0.71 -4.16
Vacuole -3.50 -14.31 -15.52 -8.39 -7.48 -4.20 -13.02 -21.05 -10.22 -2.48 -5.39 -19.30 -25.90 -13.40 -10.20
Vacuole Periphery -0.83 2.48 -1.25 0.37 -1.69 0.75 -0.44 -2.54 -2.97 -1.77 -0.76 1.74 -2.34 -0.05 -2.93
Standard name
Human Ortholog
Description Mevalonate pyrophosphate decarboxylase; essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Mvd1

Mvd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mvd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available