Standard name | |
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Human Ortholog | |
Description | Ser/Thr protein kinase; phosphorylates several aminophospholipid translocase family members, regulating phospholipid translocation and membrane asymmetry; phosphorylates and inhibits the protein kinase Akl1p, stimulating endocytosis; phosphorylates and inhibits upstream inhibitory kinase, Ypk1p; localizes to the cytoplasm, early endosome/TGN, the plasma membrane and the shmoo tip; redundant role with KIN82 in the mating pheromone response; activity stimulated by MIPC, a complex sphingolipid |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 4 | 2 | 5 | 0 | 2 | 1 | 1 | – | 1 | 1 | 2 | 4 | 0 | 0 | 0 | 1 | 2 | 6 | 14 | 5 | 4 | 3 | 7 | 15 |
Bud | 3 | 2 | 3 | 1 | 5 | 2 | 8 | – | 6 | 8 | 9 | 11 | 5 | 2 | 3 | 5 | 0 | 1 | 1 | 5 | 2 | 1 | 3 | 9 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 8 | 12 | 12 | 10 | 9 | 10 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 48 | 80 | 48 | 42 | 30 | 23 | 17 | – | 6 | 15 | 20 | 13 | 19 | 31 | 41 | 67 | 79 | 33 | 11 | 1 | 7 | 17 | 30 | 32 |
Cytoplasm | 233 | 351 | 264 | 218 | 251 | 208 | 250 | – | 104 | 102 | 171 | 133 | 183 | 195 | 235 | 357 | 315 | 314 | 204 | 369 | 247 | 99 | 106 | 127 |
Endoplasmic Reticulum | 8 | 17 | 2 | 0 | 2 | 4 | 4 | – | 1 | 0 | 0 | 2 | 0 | 0 | 3 | 13 | 11 | 7 | 7 | 6 | 4 | 12 | 9 | 11 |
Endosome | 1 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 4 | 2 | 2 | 0 | 1 | 0 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 6 | 2 | 0 | 0 | 1 | 0 |
Mitochondria | 4 | 2 | 0 | 15 | 8 | 27 | 49 | – | 40 | 53 | 100 | 70 | 0 | 0 | 1 | 10 | 5 | 1 | 3 | 3 | 6 | 6 | 3 | 4 |
Nucleus | 0 | 3 | 2 | 1 | 0 | 1 | 1 | – | 3 | 1 | 1 | 1 | 0 | 1 | 0 | 6 | 20 | 7 | 0 | 0 | 2 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 4 | 9 | 1 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 0 | 0 | 3 | 5 | 7 | 4 | – | 1 | 4 | 4 | 4 | 0 | 0 | 0 | 25 | 28 | 28 | 2 | 6 | 2 | 3 | 2 | 3 |
Unique Cell Count | 253 | 386 | 274 | 227 | 267 | 235 | 292 | 138 | 145 | 234 | 196 | 184 | 197 | 239 | 410 | 381 | 367 | 271 | 418 | 299 | 157 | 180 | 223 | |
Labelled Cell Count | 304 | 457 | 326 | 280 | 303 | 273 | 335 | 163 | 186 | 307 | 239 | 207 | 229 | 285 | 488 | 468 | 407 | 271 | 418 | 299 | 157 | 180 | 223 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.9 | 8.4 | 7.7 | 7.1 | 7.5 | 6.2 | 6.0 | – | 5.5 | 5.2 | 5.0 | 5.3 | 7.3 | 7.8 | 7.7 | 10.2 | 10.5 | 9.3 | 6.7 | 7.1 | 7.6 |
Std Deviation (1e-4) | 0.9 | 1.0 | 1.3 | 1.5 | 1.4 | 1.3 | 1.1 | – | 1.6 | 1.4 | 1.3 | 1.2 | 0.8 | 1.2 | 1.1 | 2.8 | 3.0 | 2.6 | 1.0 | 1.5 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.04 | -0.3 | -0.35 | – | -0.47 | -0.56 | -0.63 | -0.53 | -0.07 | 0.03 | 0.02 | 0.41 | 0.45 | 0.28 | -0.19 | -0.11 | -0.0 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 1.4 | – | 0 | 0 | 2.0 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.3 | -2.1 | -2.5 | -4.4 | – | -3.7 | -2.0 | -3.0 | -3.5 | -2.1 | -0.5 | -0.1 | -0.4 | 1.0 | -3.2 |
Cytoplasm | -0.2 | -1.3 | -3.4 | -4.4 | – | -6.5 | -7.6 | -7.5 | -8.4 | 2.1 | 1.8 | 1.4 | -4.1 | -5.4 | -4.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.1 | 2.0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.3 | 0 | 5.8 | 7.1 | – | 9.4 | 10.7 | 12.1 | 10.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.2 | 4.6 | 4.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.3645 | 6.4832 | 5.9557 | 6.0746 | 4.8705 | 6.036 | 3.9926 | 4.9726 | 4.363 | 4.1546 | 3.3758 | 4.4741 | 3.4068 | 4.7549 | 3.7669 | 3.8871 | 3.2787 | 3.8405 |
Actin | 0.0278 | 0.0087 | 0.0131 | 0.015 | 0.0303 | 0.0008 | 0.0117 | 0.0058 | 0.0225 | 0.0124 | 0.0035 | 0.0154 | 0.0452 | 0.0024 | 0.0312 | 0.0382 | 0.0011 | 0.009 |
Bud | 0.0003 | 0.0002 | 0.001 | 0.0004 | 0.0005 | 0.0002 | 0.0055 | 0.0013 | 0.0016 | 0.0157 | 0.0012 | 0.0124 | 0.0029 | 0.0029 | 0.0157 | 0.0178 | 0.003 | 0.0009 |
Bud Neck | 0.0021 | 0.0005 | 0.0025 | 0.0124 | 0.0014 | 0.0115 | 0.0091 | 0.0008 | 0.0072 | 0.0022 | 0.0314 | 0.0285 | 0.0123 | 0.0008 | 0.0033 | 0.0016 | 0.001 | 0.0136 |
Bud Periphery | 0.0007 | 0.0005 | 0.0024 | 0.001 | 0.001 | 0.0005 | 0.0047 | 0.0011 | 0.0025 | 0.0051 | 0.0014 | 0.0097 | 0.0035 | 0.0013 | 0.0175 | 0.014 | 0.0029 | 0.0017 |
Bud Site | 0.0052 | 0.0088 | 0.0085 | 0.0021 | 0.0015 | 0.0004 | 0.0151 | 0.0484 | 0.0153 | 0.0093 | 0.0119 | 0.0022 | 0.0297 | 0.0421 | 0.0363 | 0.0155 | 0.0031 | 0.001 |
Cell Periphery | 0.0174 | 0.0099 | 0.0099 | 0.0044 | 0.0032 | 0.0066 | 0.0101 | 0.0035 | 0.0069 | 0.0021 | 0.0008 | 0.0036 | 0.0073 | 0.0035 | 0.0028 | 0.0016 | 0.0019 | 0.0064 |
Cytoplasm | 0.8215 | 0.9232 | 0.8882 | 0.9291 | 0.8256 | 0.9385 | 0.8722 | 0.9204 | 0.8824 | 0.8095 | 0.6922 | 0.8995 | 0.7107 | 0.9236 | 0.7846 | 0.8184 | 0.9059 | 0.9302 |
Cytoplasmic Foci | 0.0117 | 0.0016 | 0.0059 | 0.0042 | 0.0028 | 0.0016 | 0.0093 | 0.0047 | 0.0102 | 0.0191 | 0.0436 | 0.003 | 0.0287 | 0.0082 | 0.019 | 0.0121 | 0.0103 | 0.004 |
Eisosomes | 0.0009 | 0.0003 | 0.0008 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0 | 0.0015 | 0.0002 | 0 | 0.0002 | 0.0004 | 0 | 0.0003 | 0.0002 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0093 | 0.0021 | 0.0024 | 0.002 | 0.0063 | 0.0025 | 0.0082 | 0.002 | 0.0053 | 0.0038 | 0.0023 | 0.0021 | 0.0134 | 0.0013 | 0.0037 | 0.0031 | 0.0013 | 0.0037 |
Endosome | 0.0141 | 0.0007 | 0.0016 | 0.0019 | 0.0185 | 0.0009 | 0.0121 | 0.0018 | 0.0118 | 0.0367 | 0.0786 | 0.0055 | 0.037 | 0.0037 | 0.0143 | 0.0043 | 0.0086 | 0.0057 |
Golgi | 0.0038 | 0.0004 | 0.0013 | 0.0024 | 0.0051 | 0.0003 | 0.004 | 0.0007 | 0.006 | 0.0101 | 0.0296 | 0.0034 | 0.0154 | 0.0015 | 0.0067 | 0.0065 | 0.0148 | 0.0025 |
Lipid Particles | 0.0041 | 0.0003 | 0.0014 | 0.0012 | 0.0028 | 0.0006 | 0.0036 | 0.0004 | 0.0021 | 0.0057 | 0.0405 | 0.0015 | 0.0126 | 0.0002 | 0.0108 | 0.0032 | 0.0091 | 0.0033 |
Mitochondria | 0.003 | 0.0039 | 0.0028 | 0.0004 | 0.0045 | 0.0001 | 0.0009 | 0.0001 | 0.0044 | 0.0064 | 0.0086 | 0.0046 | 0.0225 | 0.0001 | 0.0046 | 0.0097 | 0.0026 | 0.0024 |
None | 0.0581 | 0.0354 | 0.0455 | 0.0139 | 0.0731 | 0.0343 | 0.0059 | 0.0047 | 0.0078 | 0.0149 | 0.0081 | 0.0039 | 0.0285 | 0.0053 | 0.0296 | 0.0318 | 0.0207 | 0.0097 |
Nuclear Periphery | 0.0031 | 0.0002 | 0.0003 | 0.0002 | 0.0078 | 0.0001 | 0.002 | 0.0002 | 0.0006 | 0.0026 | 0.0036 | 0.0006 | 0.0044 | 0.0002 | 0.0037 | 0.0006 | 0.0002 | 0.0011 |
Nucleolus | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0016 | 0 | 0.0006 | 0.0001 | 0.0001 | 0.0031 | 0.0018 | 0.0001 | 0.0003 | 0 | 0.0003 | 0.0002 | 0.0002 | 0.0001 |
Nucleus | 0.0009 | 0.0003 | 0.0006 | 0.0004 | 0.0064 | 0.0002 | 0.009 | 0.0007 | 0.0012 | 0.0105 | 0.0022 | 0.0008 | 0.0011 | 0.0005 | 0.0032 | 0.0011 | 0.0009 | 0.0007 |
Peroxisomes | 0.0036 | 0.0019 | 0.0093 | 0.0022 | 0.0008 | 0 | 0.0081 | 0.0001 | 0.0069 | 0.0011 | 0.0174 | 0.0007 | 0.0147 | 0.0002 | 0.0074 | 0.015 | 0.0075 | 0.0009 |
Punctate Nuclear | 0.009 | 0.0001 | 0.0003 | 0.0002 | 0.003 | 0 | 0.0042 | 0.0002 | 0.0006 | 0.0042 | 0.0153 | 0.0004 | 0.0013 | 0.0001 | 0.0022 | 0.0007 | 0.0003 | 0.001 |
Vacuole | 0.0029 | 0.0008 | 0.0019 | 0.0063 | 0.0022 | 0.0009 | 0.0032 | 0.0029 | 0.0026 | 0.019 | 0.0048 | 0.0017 | 0.0062 | 0.002 | 0.0024 | 0.0038 | 0.0042 | 0.0016 |
Vacuole Periphery | 0.0003 | 0 | 0.0001 | 0.0001 | 0.001 | 0 | 0.0003 | 0.0001 | 0.0004 | 0.0062 | 0.0012 | 0.0003 | 0.0016 | 0.0001 | 0.0006 | 0.0004 | 0.0002 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 25.7132 | 17.2855 | 23.197 | 31.7131 | 22.4033 | 23.8486 | 29.4698 | 34.8861 | 31.4422 | 28.172 |
Translational Efficiency | 0.6623 | 0.7159 | 0.7127 | 0.5417 | 0.537 | 0.8729 | 0.573 | 0.5488 | 0.6819 | 0.5929 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1587 | 1129 | 680 | 1846 | 1530 | 2068 | 1428 | 2290 | 3117 | 3197 | 2108 | 4136 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 757.15 | 809.31 | 1290.57 | 1079.57 | 828.72 | 896.73 | 927.84 | 1103.26 | 792.28 | 865.86 | 1044.85 | 1092.69 |
Standard Deviation | 87.48 | 195.38 | 166.11 | 138.33 | 98.48 | 116.53 | 111.13 | 169.98 | 99.68 | 154.95 | 214.52 | 157.09 |
Intensity Change Log 2 | — | 0.096113 | 0.769357 | 0.511806 | — | 0.113789 | 0.162991 | 0.412816 | — | 0.105377 | 0.484252 | 0.460925 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001236 | 0.002522 | 0.003492 | 0.002465 | 0.001499 | 0.003344 | 0.000225 | 0.008034 | 0.001365 | 0.003053 | 0.001279 | 0.005549 |
Bud Neck | 0.038450 | 0.020980 | 0.004407 | 0.005922 | 0.027465 | 0.026706 | 0.005235 | 0.004598 | 0.033058 | 0.024684 | 0.004968 | 0.005189 |
Bud Site | 0.074150 | 0.076162 | 0.083508 | 0.061668 | 0.062112 | 0.083626 | 0.015571 | 0.075259 | 0.068241 | 0.080990 | 0.037486 | 0.069193 |
Cell Periphery | 0.001508 | 0.001293 | 0.000925 | 0.000305 | 0.001823 | 0.001266 | 0.000213 | 0.000565 | 0.001662 | 0.001276 | 0.000443 | 0.000449 |
Cytoplasm | 0.615099* | 0.582742* | 0.683097* | 0.714509* | 0.647843* | 0.640884* | 0.514946* | 0.793544* | 0.631172* | 0.620352* | 0.569188* | 0.758269* |
Cytoplasmic Foci | 0.141313 | 0.147873 | 0.098058 | 0.043294 | 0.144399 | 0.131271 | 0.058635 | 0.033734 | 0.142827 | 0.137134 | 0.071352 | 0.038001 |
Eisosomes | 0.001082 | 0.000598 | 0.000053 | 0.000062 | 0.001157 | 0.001015 | 0.000122 | 0.000096 | 0.001119 | 0.000868 | 0.000099 | 0.000081 |
Endoplasmic Reticulum | 0.002010 | 0.001608 | 0.000673 | 0.000410 | 0.002637 | 0.000859 | 0.000560 | 0.000080 | 0.002318 | 0.001124 | 0.000596 | 0.000227 |
Endosome | 0.012485 | 0.026981 | 0.003247 | 0.004639 | 0.010877 | 0.012492 | 0.010353 | 0.001455 | 0.011696 | 0.017609 | 0.008061 | 0.002876 |
Golgi | 0.011527 | 0.006552 | 0.000582 | 0.001540 | 0.011877 | 0.014216 | 0.001102 | 0.000441 | 0.011699 | 0.011510 | 0.000934 | 0.000931 |
Lipid Particles | 0.003678 | 0.006390 | 0.003456 | 0.000599 | 0.003544 | 0.002599 | 0.001988 | 0.000667 | 0.003612 | 0.003938 | 0.002461 | 0.000636 |
Mitochondria | 0.002307 | 0.001201 | 0.000019 | 0.001069 | 0.002912 | 0.002732 | 0.001306 | 0.000080 | 0.002604 | 0.002191 | 0.000891 | 0.000521 |
Mitotic Spindle | 0.000317 | 0.000427 | 0.000842 | 0.009798 | 0.000571 | 0.002170 | 0.003449 | 0.002424 | 0.000441 | 0.001554 | 0.002608 | 0.005715 |
None | 0.002813 | 0.005811 | 0.000649 | 0.000170 | 0.001522 | 0.000997 | 0.001753 | 0.000313 | 0.002180 | 0.002697 | 0.001397 | 0.000249 |
Nuclear Periphery | 0.000089 | 0.000547 | 0.000072 | 0.000072 | 0.000049 | 0.000028 | 0.000144 | 0.000019 | 0.000069 | 0.000211 | 0.000120 | 0.000043 |
Nuclear Periphery Foci | 0.000531 | 0.002014 | 0.008900 | 0.000786 | 0.000785 | 0.000396 | 0.000925 | 0.000494 | 0.000656 | 0.000967 | 0.003497 | 0.000625 |
Nucleolus | 0.000599 | 0.001358 | 0.000039 | 0.000111 | 0.000191 | 0.000163 | 0.000255 | 0.000062 | 0.000399 | 0.000585 | 0.000185 | 0.000084 |
Nucleus | 0.017046 | 0.024525 | 0.017474 | 0.006544 | 0.013453 | 0.009530 | 0.035292 | 0.005962 | 0.015283 | 0.014825 | 0.029544 | 0.006222 |
Peroxisomes | 0.003232 | 0.002675 | 0.000747 | 0.003707 | 0.002157 | 0.004530 | 0.001924 | 0.004385 | 0.002704 | 0.003875 | 0.001545 | 0.004082 |
Vacuole | 0.068632 | 0.085778 | 0.089634 | 0.139696 | 0.061206 | 0.059357 | 0.345070* | 0.067673 | 0.064987 | 0.068688 | 0.262672* | 0.099819 |
Vacuole Periphery | 0.001897 | 0.001964 | 0.000128 | 0.002630 | 0.001920 | 0.001818 | 0.000933 | 0.000116 | 0.001909 | 0.001869 | 0.000673 | 0.001238 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.90 | -6.40 | -2.61 | 1.13 | 2.89 | -6.09 | 11.62 | -5.43 | -3.35 | -6.83 | -7.83 | 1.13 | -5.94 | -2.69 | -6.15 |
Bud Neck | 6.84 | 15.85 | 15.43 | 10.61 | -1.31 | 0.36 | 12.59 | 13.18 | 16.91 | 1.59 | 4.95 | 20.05 | 20.30 | 19.81 | 0.44 |
Bud Site | -0.32 | 0.52 | 5.33 | 5.38 | 3.55 | -5.71 | 14.51 | -0.93 | 4.96 | -16.38 | -4.12 | 11.76 | 3.09 | 7.72 | -9.79 |
Cell Periphery | 1.63 | 6.57 | 13.53 | 9.72 | 8.99 | 4.94 | 16.59 | 11.44 | 9.05 | -4.58 | 4.64 | 18.30 | 16.86 | 13.92 | 0.15 |
Cytoplasm | 3.63 | -2.12 | -10.05 | -12.34 | -5.19 | 1.03 | 15.94 | -17.62 | -19.52 | -30.64 | 2.04 | 11.68 | -19.92 | -21.50 | -26.93 |
Cytoplasmic Foci | -1.27 | 7.96 | 26.35 | 23.24 | 14.16 | 2.94 | 22.86 | 29.93 | 28.72 | 7.70 | 1.63 | 23.96 | 39.88 | 36.77 | 14.08 |
Eisosomes | 6.22 | 16.89 | 16.87 | 10.89 | -0.11 | 1.60 | 14.45 | 14.84 | 17.12 | 3.12 | 4.14 | 21.73 | 22.22 | 20.22 | 3.89 |
Endoplasmic Reticulum | 0.90 | 3.83 | 4.52 | 3.81 | 2.25 | 3.28 | 4.01 | 4.86 | 5.77 | 6.98 | 3.53 | 5.56 | 6.70 | 6.05 | 4.49 |
Endosome | -7.91 | 12.43 | 11.00 | 13.18 | -1.00 | -2.00 | 1.51 | 17.30 | 17.03 | 11.27 | -6.97 | 6.35 | 19.36 | 19.71 | 9.57 |
Golgi | 6.05 | 15.86 | 14.11 | 10.50 | -2.34 | -2.82 | 17.31 | 21.27 | 22.05 | 1.79 | 0.32 | 22.04 | 24.12 | 23.74 | 0.46 |
Lipid Particles | -2.69 | -0.85 | 13.60 | 5.81 | 10.73 | 2.82 | 4.25 | 10.76 | 7.13 | 2.77 | -0.84 | 3.72 | 16.84 | 8.35 | 6.75 |
Mitochondria | 2.07 | 6.43 | 3.38 | 0.93 | -2.53 | 0.18 | 1.67 | 3.21 | 5.00 | 1.76 | 0.69 | 2.94 | 4.57 | 4.62 | 0.93 |
Mitotic Spindle | -0.40 | -2.93 | -5.01 | -4.90 | -4.64 | -2.23 | -2.20 | -3.64 | -1.34 | -0.40 | -2.41 | -2.53 | -6.21 | -4.40 | -3.06 |
None | -2.15 | 5.32 | 6.37 | 4.26 | 3.65 | 2.01 | -0.09 | 6.08 | 4.30 | 3.30 | -0.97 | 2.73 | 8.30 | 5.11 | 3.80 |
Nuclear Periphery | -2.79 | -0.57 | 0.53 | 2.92 | 2.39 | 1.64 | -5.69 | 1.34 | -0.89 | 9.22 | -2.39 | -3.52 | 1.13 | 2.78 | 8.06 |
Nuclear Periphery Foci | -4.74 | -14.85 | -1.78 | 3.85 | 14.32 | 5.66 | -0.94 | 3.17 | -2.47 | 3.66 | -2.61 | -13.48 | 0.32 | 2.62 | 13.35 |
Nucleolus | -2.32 | 2.46 | 2.19 | 5.63 | -3.86 | 1.98 | -0.95 | 9.51 | 10.70 | 3.67 | -1.26 | 1.91 | 2.71 | 6.29 | 2.81 |
Nucleus | -3.29 | -2.62 | 12.64 | 7.49 | 11.17 | 4.75 | -13.75 | 10.40 | 4.30 | 19.28 | 0.27 | -12.99 | 16.47 | 8.47 | 21.75 |
Peroxisomes | 1.63 | 7.85 | 0.33 | -0.77 | -4.90 | -6.11 | 0.77 | -3.11 | 1.18 | -2.83 | -3.95 | 3.34 | -1.95 | 0.99 | -4.12 |
Vacuole | -4.35 | -10.10 | -20.88 | -15.44 | -4.11 | 0.72 | -41.88 | -10.40 | -11.40 | 35.21* | -1.80 | -38.63 | -21.84 | -20.22 | 25.86 |
Vacuole Periphery | -0.23 | 8.10 | 0.03 | 0.14 | -3.17 | 0.24 | 2.15 | 5.65 | 5.84 | 1.76 | 0.15 | 3.96 | 3.21 | 3.04 | -0.83 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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