Standard name
Human Ortholog
Description Putative GTPase; associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation; recruited by ribosomal proteins L17, L35, and L37 to assembling ribosomes after 27SB pre-rRNA is generated, immediately preceding removal of ITS2
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 – 0.18 0.05 0.06 0 0.07 0 0 0 0 0.06 0.1 0.11 0.06 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 – 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – 0 0 0 0 0 0.06 0 0.13 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.98 0.94 – 0.45 0.29 0.54 0.68 0.75 0.65 0.81 0.69 0.74 0.77 0.5 0.23 0.86 0.86 0.86 0.96 0.9 0.8 0.94 0.73 0.63
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0.36 0.63 0.39 0.23 0.19 0.32 0.18 0.27 0.22 0.19 0.42 0.66 0.06 0.09 0.06 0 0 0 0 0.11 0.1
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 – 0 0.06 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.23
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 1 0 1 0 0 3
Bud Neck 0 0 – 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 1 0 2 1 2 4 0 1 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
Cytoplasm 1 2 – 4 4 5 5 10 4 6 4 4 10 20 29 16 10 12 0 0 0 0 0 1
Endoplasmic Reticulum 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 2 2 0 0 0 0 0 0
Endosome 0 0 – 0 1 5 3 2 1 1 0 4 1 3 6 0 0 1 1 0 0 0 1 1
Golgi 0 0 – 0 2 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0
Mitochondria 0 1 – 0 0 4 5 1 8 4 21 6 0 4 2 5 5 6 0 1 1 0 1 0
Nucleus 361 95 – 10 24 49 113 113 80 133 109 128 131 103 61 231 232 245 358 102 35 65 162 165
Nuclear Periphery 0 0 – 0 1 3 2 4 4 4 4 3 0 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 3 1 – 8 53 35 39 28 40 30 43 38 32 85 178 15 23 17 6 2 1 1 23 25
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 – 0 1 0 1 0 0 0 1 1 0 3 6 0 0 0 0 0 0 0 1 1
Vac/Vac Membrane 4 5 – 0 5 3 10 6 5 1 3 7 4 2 5 12 11 10 3 3 1 0 28 60
Unique Cell Count 369 101 22 84 90 166 150 124 165 157 173 170 204 271 270 269 284 372 113 44 70 223 264
Labelled Cell Count 370 104 22 91 104 180 164 145 180 187 196 178 222 294 280 284 293 372 113 44 70 223 264


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 22.3 32.0 – 21.9 19.6 11.4 10.4 9.5 7.8 8.5 7.6 8.7 26.4 22.6 20.5 31.1 31.0 33.3 25.7 23.9 24.0
Std Deviation (1e-4) 5.0 9.4 – 8.9 6.8 2.9 3.1 2.8 2.0 2.5 1.9 2.9 9.4 8.5 6.9 13.1 11.8 13.7 8.2 6.7 7.8
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 18.0345 31.5558 27.2758 29.3518 28.434 29.4184 18.0288 41.3028 36.8607 32.3179 33.1027 35.0711 19.6303 33.8297 29.7295 28.3566 26.9116 27.4401
Actin 0.0837 0.0043 0.0115 0.0014 0.0484 0.0001 0.0192 0.0001 0.0048 0 0.0002 0.0001 0.0287 0 0.0001 0 0.0191 0.0054
Bud 0.0014 0.0002 0.0004 0.0001 0.0006 0.0003 0.0032 0.0001 0.0006 0.0004 0.0012 0.0002 0.0004 0 0.0006 0 0.0001 0.0001
Bud Neck 0.001 0.0001 0.0003 0.0001 0.0003 0.0009 0.0007 0.0002 0.0041 0.0001 0.0003 0.0009 0.002 0.0001 0.0001 0.0001 0.0002 0.0009
Bud Periphery 0.0014 0.0003 0.0005 0.0004 0.0007 0.001 0.0033 0.0001 0.0009 0.0008 0.002 0.0006 0.0005 0.0001 0.0002 0.0001 0.0001 0.0004
Bud Site 0.0052 0.0012 0.0008 0.0002 0.0004 0.0003 0.0086 0.005 0.0038 0.0001 0.0001 0.0001 0.0017 0 0.0001 0 0.0001 0.0002
Cell Periphery 0.0014 0.001 0.0008 0.0001 0.0002 0.002 0.0027 0.0009 0.001 0.003 0.0012 0.002 0.0009 0.0007 0.0003 0.0003 0.0001 0.001
Cytoplasm 0.0166 0.0005 0.0029 0.0001 0.0007 0.0001 0.0121 0.0001 0.0006 0.0003 0.0014 0.0003 0.0043 0.0001 0.0033 0.0001 0.0002 0.0002
Cytoplasmic Foci 0.0367 0.0032 0.0149 0.0006 0.0021 0.0001 0.1046 0 0.0053 0.0025 0.0002 0.001 0.0174 0 0.0059 0.0002 0.0015 0.004
Eisosomes 0.0006 0 0.0001 0 0 0 0.0002 0 0 0 0 0 0.0009 0 0 0 0.0002 0
Endoplasmic Reticulum 0.0066 0.0006 0.0019 0.0001 0.0014 0 0.0054 0 0.0006 0 0.0001 0 0.0013 0 0 0 0.0004 0.0002
Endosome 0.0356 0.0009 0.0108 0.0031 0.0217 0.0001 0.0437 0 0.0032 0 0.0001 0.003 0.0051 0 0.0009 0 0.0034 0.0013
Golgi 0.0058 0.0003 0.0053 0.0024 0.006 0 0.0102 0 0.004 0 0 0.0021 0.0031 0 0.0004 0 0.0023 0.001
Lipid Particles 0.0175 0.0003 0.0065 0.0027 0.0013 0.0004 0.0643 0 0.0014 0.0014 0.0002 0.0007 0.0069 0 0.0018 0.0001 0.0074 0.0021
Mitochondria 0.0135 0.0025 0.0135 0.0391 0.014 0.0011 0.0078 0.0009 0.0041 0.0033 0.0201 0.0076 0.0138 0.0006 0.0009 0.0004 0.0011 0.0013
None 0.0173 0.0002 0.0022 0.0001 0.0003 0 0.0131 0 0.0004 0.0004 0.0006 0.0001 0.0138 0 0.0002 0.0001 0.0013 0.0002
Nuclear Periphery 0.0237 0.008 0.0057 0.0023 0.0045 0.0008 0.052 0.0061 0.0054 0.0006 0.0027 0.0026 0.0034 0.007 0.0027 0.002 0.0057 0.0062
Nucleolus 0.1627 0.0893 0.1842 0.1173 0.183 0.1706 0.0698 0.1101 0.2746 0.1363 0.2522 0.2672 0.2605 0.1039 0.2139 0.1269 0.1831 0.1947
Nucleus 0.4956 0.877 0.7201 0.8262 0.7082 0.8121 0.5086 0.8724 0.6764 0.8387 0.6962 0.7005 0.601 0.8825 0.7623 0.8511 0.7638 0.7689
Peroxisomes 0.0076 0.0002 0.0046 0.0014 0.0017 0.0001 0.0343 0 0.0017 0.0001 0.0005 0.0011 0.0124 0 0.0032 0 0.0027 0.0012
Punctate Nuclear 0.0432 0.0033 0.0041 0.0002 0.0004 0.0001 0.0177 0.0001 0.0009 0.0001 0.0001 0.0001 0.0166 0.0001 0.0011 0.0165 0.0067 0.006
Vacuole 0.0115 0.0022 0.0035 0.0007 0.0013 0.0047 0.0112 0.0019 0.0024 0.0058 0.0045 0.0047 0.0022 0.0019 0.001 0.0009 0.0002 0.0025
Vacuole Periphery 0.0115 0.0042 0.0053 0.0013 0.0026 0.0053 0.0074 0.0021 0.0039 0.006 0.016 0.0051 0.0029 0.0028 0.001 0.0013 0.0005 0.0021

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 112.153 114.0983 122.2394 84.7466 110.6497 73.9725 80.8918 54.5965 60.4851 95.0003
Translational Efficiency 1.2348 1.1728 0.9485 1.046 1.0844 1.2031 1.0711 1.2538 0.8457 1.0512

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1911 1992 145 989 1814 1783 167 338 3725 3775 312 1327

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1496.15 1825.34 2470.01 2385.19 1771.26 1968.08 2595.56 2580.54 1630.12 1892.76 2537.21 2434.95
Standard Deviation 341.97 594.59 492.69 719.87 393.68 475.93 398.96 620.65 392.91 546.46 449.37 701.12
Intensity Change Log 2 — 0.286910 0.723262 0.672849 — 0.152013 0.551270 0.542897 — 0.215352 0.632577 0.603858

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000625 0.000663 0.000995 0.001162 0.000809 0.000755 0.000861 0.000848 0.000715 0.000706 0.000923 0.001082
Bud Neck 0.001612 0.001687 0.007730 0.016720 0.002619 0.001958 0.008364 0.016343 0.002103 0.001815 0.008070 0.016624
Bud Site 0.000397 0.000600 0.000708 0.003778 0.000270 0.000517 0.000616 0.001551 0.000335 0.000561 0.000659 0.003211
Cell Periphery 0.000377 0.000634 0.000027 0.000277 0.000136 0.000599 0.000022 0.000087 0.000260 0.000617 0.000025 0.000229
Cytoplasm 0.000793 0.001319 0.000020 0.001238 0.000027 0.000467 0.000042 0.000326 0.000420 0.000917 0.000032 0.001006
Cytoplasmic Foci 0.000292 0.000609 0.000283 0.002912 0.000163 0.000380 0.000301 0.004366 0.000229 0.000501 0.000293 0.003282
Eisosomes 0.000593 0.000307 0.000242 0.000110 0.000447 0.000528 0.000169 0.000090 0.000522 0.000411 0.000203 0.000105
Endoplasmic Reticulum 0.001132 0.000865 0.000347 0.000601 0.000872 0.001014 0.000312 0.000253 0.001005 0.000935 0.000328 0.000512
Endosome 0.000021 0.000328 0.000008 0.001993 0.000035 0.000119 0.000010 0.001925 0.000027 0.000229 0.000009 0.001975
Golgi 0.000018 0.000151 0.000037 0.001162 0.000053 0.000064 0.000050 0.001726 0.000035 0.000110 0.000044 0.001306
Lipid Particles 0.000005 0.000154 0.000013 0.000101 0.000039 0.000046 0.000007 0.002036 0.000022 0.000103 0.000010 0.000593
Mitochondria 0.000725 0.001117 0.002392 0.004721 0.001076 0.001303 0.001998 0.002908 0.000896 0.001204 0.002181 0.004259
Mitotic Spindle 0.000011 0.000385 0.000015 0.004893 0.000250 0.000200 0.000065 0.000772 0.000128 0.000297 0.000042 0.003843
None 0.002833 0.004048 0.000388 0.002075 0.000676 0.000798 0.000462 0.001945 0.001782 0.002513 0.000428 0.002042
Nuclear Periphery 0.000034 0.000060 0.000035 0.000521 0.000050 0.000080 0.000054 0.000233 0.000042 0.000070 0.000045 0.000448
Nuclear Periphery Foci 0.000038 0.000077 0.000002 0.000264 0.000068 0.000292 0.000005 0.000122 0.000053 0.000178 0.000004 0.000228
Nucleolus 0.023008 0.016063 0.063890 0.191947 0.104667 0.024454 0.166622 0.206405 0.062774 0.020026 0.118878 0.195630
Nucleus 0.962428* 0.965217* 0.911549* 0.753231* 0.882807* 0.962751* 0.810064* 0.748317* 0.923654* 0.964052* 0.857229* 0.751979*
Peroxisomes 0.000740 0.000837 0.010677 0.004784 0.002315 0.001098 0.009026 0.005954 0.001507 0.000960 0.009793 0.005082
Vacuole 0.003637 0.003990 0.000464 0.004181 0.001605 0.002110 0.000526 0.001592 0.002647 0.003102 0.000497 0.003522
Vacuole Periphery 0.000683 0.000890 0.000178 0.003328 0.001014 0.000469 0.000422 0.002201 0.000844 0.000691 0.000309 0.003041

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.85 -4.10 -5.97 -5.61 -0.96 1.17 -1.09 -1.81 -2.51 -0.47 0.30 -4.02 -5.68 -5.77 -1.71
Bud Neck -0.21 -7.62 -11.76 -11.50 -6.31 2.60 -6.65 -7.52 -7.78 -4.28 1.27 -9.96 -13.74 -13.86 -7.40
Bud Site -1.10 -1.48 -3.99 -3.64 -3.63 -1.69 -3.36 -2.55 -2.21 -2.15 -1.90 -2.96 -4.76 -4.27 -4.22
Cell Periphery -1.39 5.76 1.42 2.08 -4.52 -1.50 2.21 0.31 1.57 -2.06 -2.03 5.89 0.59 2.22 -4.94
Cytoplasm -0.96 2.15 -1.03 -0.34 -2.02 -2.02 -0.64 -2.60 1.11 -2.45 -1.96 2.09 -1.49 -0.28 -2.16
Cytoplasmic Foci -1.07 0.24 -3.65 -3.25 -3.78 -1.15 -1.21 -2.45 -2.28 -2.30 -1.58 -0.77 -4.46 -4.02 -4.31
Eisosomes 3.13 3.95 5.65 6.03 6.37 -1.23 5.17 6.80 10.62 4.23 1.97 6.08 8.20 11.64 6.91
Endoplasmic Reticulum 2.26 7.89 3.24 1.74 -1.46 -1.14 6.82 7.39 6.58 0.28 0.80 10.44 4.05 3.40 -1.56
Endosome -1.73 2.22 -2.46 -1.94 -2.48 -1.12 0.94 -1.55 -1.46 -1.57 -2.01 1.52 -2.88 -2.48 -2.92
Golgi -1.60 -1.03 -2.88 -2.48 -2.84 -0.24 -0.21 -1.76 -1.75 -1.75 -1.50 -0.56 -3.34 -3.11 -3.31
Lipid Particles -1.36 -1.27 -3.09 0.45 -2.93 -0.18 0.88 -1.05 -1.04 -1.07 -1.32 0.72 -1.21 -0.99 -1.24
Mitochondria -3.16 -6.70 -9.74 -8.43 -4.45 -0.77 -4.71 -7.67 -4.46 -3.15 -1.95 -8.23 -10.88 -8.83 -5.70
Mitotic Spindle -1.49 -0.68 -3.04 -2.76 -3.03 0.23 0.65 -2.17 -2.76 -3.08 -0.98 0.58 -3.14 -2.99 -3.21
None -1.30 4.34 1.21 2.49 -4.55 -1.13 1.47 -1.64 -1.40 -1.92 -1.53 4.52 -0.46 1.08 -4.75
Nuclear Periphery -2.10 0.04 -5.07 -4.84 -5.09 -1.60 -0.66 -4.28 -3.45 -4.09 -2.45 -0.59 -5.72 -5.32 -5.65
Nuclear Periphery Foci -1.08 1.89 -3.34 -1.41 -4.30 -1.11 1.09 -0.77 0.62 -1.49 -1.47 1.79 -3.09 -0.39 -4.49
Nucleolus 3.30 -3.94 -19.41* -20.32* -9.50 16.93* -3.55 -6.83 -11.92 -1.75 16.25* -5.06 -17.19 -23.29* -5.45
Nucleus -0.75 4.25 21.71 21.79 10.34 -15.41 3.99 8.51 13.14 2.60 -12.90 5.64 20.46 25.50 7.00
Peroxisomes -0.54 -4.76 -6.92 -6.45 2.80 5.84 -3.65 -4.47 -5.62 1.35 3.72 -5.93 -7.47 -8.42 3.27
Vacuole -0.56 5.03 -0.53 -0.03 -4.67 -1.68 4.30 -0.60 -0.01 -1.79 -1.10 6.17 -1.42 -0.64 -4.95
Vacuole Periphery -0.89 3.42 -4.50 -4.07 -5.64 3.96 4.45 -1.34 -2.07 -2.12 1.11 5.31 -4.52 -4.85 -5.82
Standard name
Human Ortholog
Description Putative GTPase; associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation; recruited by ribosomal proteins L17, L35, and L37 to assembling ribosomes after 27SB pre-rRNA is generated, immediately preceding removal of ITS2
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Nog2

Nog2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nog2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available