Standard name
Human Ortholog
Description Endosomal protein involved in turnover of plasma membrane proteins; member of the DUP380 subfamily of conserved, often subtelomeric COS genes; required for the multivesicular vesicle body sorting pathway that internalizes plasma membrane proteins for degradation; Cos proteins provide ubiquitin in trans for nonubiquitinated cargo proteins
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.95 0.93 0.96 0.98 1.0 0.95 0.94 0.94 0.94 0.95 0.86 0.89 0.98 1.0 1.0 – – – 0.41 0.39 0.35 0.53 0.44 0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.12 0.08 0.08 0.05 0.05 0.08
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 0 0 0.07 0.06 0.11 0.08 0.23 0.11 0 0 0 – – – 0.06 0.05 0.1 0.05 0 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.27 0.35 0.33 0.32 0.4 0.38
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 0 0 0 0 1 3 1 2 0 0 0 0 0 – – – 13 5 3 1 1 1
Bud 3 0 0 3 1 8 15 10 9 10 13 13 0 0 0 – – – 17 4 5 1 5 8
Bud Neck 2 0 2 3 4 2 4 6 4 11 2 6 1 1 2 – – – 6 3 2 0 1 2
Bud Site 0 0 0 0 0 0 0 1 1 0 2 1 0 0 0 – – – – – – – – –
Cell Periphery 1 1 2 4 1 1 1 1 1 1 1 2 1 7 0 – – – 4 0 1 0 0 1
Cytoplasm 673 229 222 338 400 429 526 611 443 486 303 326 109 339 288 – – – 293 105 86 74 130 94
Endoplasmic Reticulum 10 4 0 0 1 0 1 0 0 0 0 5 1 0 0 – – – 87 21 20 6 15 18
Endosome 0 0 0 1 0 4 2 0 0 2 0 2 0 0 0 – – – 13 7 6 0 2 2
Golgi 3 1 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 6 2 0 0 1 1
Mitochondria 33 1 1 2 1 12 40 36 53 42 82 39 0 0 0 – – – 45 13 25 6 12 11
Nucleus 0 2 1 1 2 4 2 5 2 3 3 2 0 0 0 – – – 0 0 0 0 0 1
Nuclear Periphery 2 1 0 0 0 1 0 1 1 0 0 1 0 0 0 – – – 1 2 1 0 0 1
Nucleolus 0 1 0 0 0 0 1 1 0 0 1 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 – – – 2 1 0 0 0 0
SpindlePole 1 0 0 0 0 2 3 9 4 3 5 6 0 0 0 – – – 14 1 1 1 1 1
Vac/Vac Membrane 13 12 11 5 4 9 10 5 4 5 3 4 1 0 0 – – – 192 93 81 45 119 90
Unique Cell Count 711 245 231 345 400 452 560 651 472 513 351 366 111 340 288 723 270 248 142 295 238
Labelled Cell Count 741 254 239 357 414 473 606 689 523 565 415 407 113 347 290 723 270 248 142 295 238


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 5.6 5.5 5.0 5.4 4.5 4.0 4.5 3.6 4.0 3.3 3.9 4.9 5.2 5.0 – – – 5.4 5.6 6.4
Std Deviation (1e-4) 1.0 1.9 1.7 1.6 1.7 1.8 1.3 1.4 1.0 1.4 1.1 0.9 1.2 1.0 1.2 – – – 1.4 1.3 2.2
Intensity Change (Log2) – – – -0.15 -0.03 -0.29 -0.47 -0.3 -0.62 -0.48 -0.73 -0.49 -0.17 -0.1 -0.13 – – – -0.03 0.02 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cytoplasm 1.3 4.0 -0.7 -1.2 -1.3 -1.2 -0.8 -3.9 -3.0 1.0 3.2 3.4 – – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole -2.4 -3.0 -2.0 -2.4 0 0 0 -3.0 -2.8 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.5092 -0.1884 -0.3847 -0.7586 -0.9506 -0.3939 -0.8434 0.2295 -0.2327 -0.3981 -0.515 -0.0666 -0.8346 -0.2543 -0.5713 -0.7912 -0.644 -0.4933
Actin 0.0194 0.0005 0.0004 0.0001 0.0001 0.0025 0.0046 0.0001 0.0001 0.0009 0.0005 0.0006 0.0033 0.0003 0.0008 0.0002 0.0005 0.0005
Bud 0.0004 0.0019 0.0005 0.0005 0 0.0028 0.0009 0.0019 0.0014 0.0002 0.0046 0.0017 0.0089 0.0042 0.001 0.0019 0.0013 0.0001
Bud Neck 0.0071 0.0003 0.0004 0.0002 0.0003 0.0026 0.0006 0.0002 0.0013 0.0005 0.001 0.0094 0.0012 0.003 0.0076 0.0022 0.0004 0.0006
Bud Periphery 0.0009 0.0014 0.0008 0.0008 0 0.0027 0.0014 0.0009 0.0005 0.0004 0.01 0.003 0.0037 0.002 0.0007 0.0011 0.0007 0.0001
Bud Site 0.0029 0.0136 0.0023 0.0003 0.0001 0.0007 0.0038 0.0059 0.0095 0.0002 0.0019 0.002 0.0132 0.0295 0.0064 0.0033 0.0008 0.0001
Cell Periphery 0.0008 0.0003 0.0002 0.0001 0 0.0002 0.0002 0.001 0.0002 0.0001 0.0003 0.0003 0.0005 0.0003 0.0001 0.0003 0.0001 0
Cytoplasm 0.0692 0.0683 0.0621 0.0574 0.0626 0.1244 0.0596 0.0749 0.1116 0.0652 0.077 0.1424 0.1053 0.1552 0.1501 0.2319 0.2085 0.2199
Cytoplasmic Foci 0.0144 0.0098 0.0065 0.0189 0.0066 0.0263 0.01 0.0071 0.0161 0.0234 0.0072 0.0144 0.022 0.0235 0.0154 0.0574 0.0108 0.0155
Eisosomes 0.0006 0.0002 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001 0.0001 0.0002 0
Endoplasmic Reticulum 0.0035 0.0044 0.0043 0.0008 0.0012 0.0011 0.0031 0.0015 0.0053 0.0012 0.001 0.0072 0.0037 0.0019 0.004 0.0023 0.0043 0.0046
Endosome 0.0144 0.0075 0.0122 0.0108 0.0167 0.0089 0.0022 0.0065 0.004 0.0035 0.0008 0.0059 0.0057 0.0072 0.006 0.0378 0.0043 0.0056
Golgi 0.0034 0.0003 0.0005 0.0003 0.0001 0.0011 0.0007 0.0002 0.0001 0.0004 0.0001 0.0002 0.0003 0.0002 0.0003 0.0009 0.0002 0.0001
Lipid Particles 0.003 0.0008 0.0011 0.0018 0.0003 0.0032 0.0012 0.0004 0.0008 0.0023 0.0002 0.0009 0.0011 0.0008 0.0017 0.0023 0.0004 0.0004
Mitochondria 0.0012 0.0008 0.0014 0.0013 0.0002 0.0011 0.0004 0.0003 0.0004 0.0007 0.0005 0.0005 0.0057 0.0007 0.0005 0.0009 0.0011 0.0003
None 0.8267 0.8721 0.8805 0.8845 0.8885 0.7982 0.9026 0.8883 0.8296 0.865 0.862 0.7971 0.8016 0.7453 0.7893 0.604 0.7505 0.7388
Nuclear Periphery 0.0042 0.0025 0.0024 0.0013 0.0013 0.0018 0.001 0.0011 0.0017 0.0017 0.0013 0.0025 0.0017 0.0014 0.0015 0.0015 0.0018 0.0014
Nucleolus 0.0012 0.0012 0.0012 0.0026 0.0002 0.0007 0.0005 0.0009 0.0012 0.0055 0.0019 0.0004 0.0043 0.0034 0.0007 0.0017 0.0011 0.0003
Nucleus 0.0033 0.0054 0.0041 0.0035 0.0071 0.0029 0.0009 0.0031 0.0036 0.0062 0.0064 0.003 0.0066 0.0084 0.0038 0.0028 0.0047 0.0027
Peroxisomes 0.0017 0.0003 0.0004 0.0014 0.0001 0.0013 0.0009 0.0001 0.0004 0.0028 0.0002 0.0005 0.0011 0.0007 0.0006 0.0045 0.0003 0.0004
Punctate Nuclear 0.0066 0.004 0.0067 0.009 0.0006 0.0127 0.0038 0.0017 0.009 0.0183 0.0191 0.0036 0.0039 0.009 0.0069 0.0072 0.0056 0.0048
Vacuole 0.0143 0.0039 0.0111 0.0037 0.0136 0.004 0.001 0.0035 0.0028 0.0013 0.0035 0.0038 0.0054 0.0028 0.0023 0.0352 0.0022 0.0034
Vacuole Periphery 0.0008 0.0004 0.0007 0.0006 0.0004 0.0005 0.0001 0.0002 0.0002 0.0002 0.0002 0.0006 0.0004 0.0003 0.0003 0.0007 0.0003 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.2984 8.7678 7.1567 7.7983 3.815 5.8816 7.757 6.5817 9.3698 10.6896
Translational Efficiency 0.6077 0.2512 0.4609 0.4031 1.1726 1.4166 0.6723 0.7193 0.4772 0.4227

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
40 1126 2596 1400 1464 2514 235 1491 1504 3640 2831 2891

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 892.61 734.89 771.50 758.46 686.31 724.81 909.07 1037.03 691.80 727.93 782.92 902.13
Standard Deviation 222.09 100.79 128.83 150.49 88.92 96.07 101.20 441.18 100.55 97.67 132.33 361.57
Intensity Change Log 2 — -0.280502 -0.210364 -0.234957 — 0.078742 0.405531 0.595525 — -0.113266 0.090013 0.185440

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001306 0.000387 0.000143 0.000526 0.000125 0.000282 0.000281 0.001092 0.000156 0.000314 0.000155 0.000818
Bud Neck 0.018830 0.026244 0.006540 0.007607 0.014542 0.031727 0.003676 0.035807 0.014656 0.030031 0.006303 0.022151
Bud Site 0.081299 0.017129 0.007584 0.024284 0.004518 0.016974 0.007411 0.046990 0.006560 0.017022 0.007570 0.035994
Cell Periphery 0.000725 0.000158 0.000153 0.000236 0.000269 0.000167 0.000295 0.000683 0.000281 0.000164 0.000165 0.000466
Cytoplasm 0.218369* 0.358918* 0.410620* 0.501639* 0.399948* 0.432214* 0.223614* 0.402504* 0.395119* 0.409540* 0.395097* 0.450511*
Cytoplasmic Foci 0.093473 0.235031* 0.008427 0.034642 0.171117 0.179148 0.053528 0.030418 0.169052 0.196435* 0.012171 0.032464
Eisosomes 0.000076 0.000142 0.000021 0.000057 0.000221 0.000139 0.000162 0.000061 0.000217 0.000140 0.000033 0.000059
Endoplasmic Reticulum 0.023119 0.009789 0.004737 0.013905 0.022527 0.007767 0.009890 0.012362 0.022542 0.008393 0.005164 0.013109
Endosome 0.101059 0.038755 0.001215 0.011059 0.027526 0.027210 0.011186 0.011750 0.029482 0.030781 0.002042 0.011415
Golgi 0.005230 0.004197 0.000056 0.000497 0.002159 0.003755 0.000271 0.005004 0.002240 0.003892 0.000074 0.002822
Lipid Particles 0.008999 0.011029 0.001077 0.005143 0.019102 0.010629 0.008736 0.003454 0.018833 0.010753 0.001713 0.004272
Mitochondria 0.020966 0.006974 0.000610 0.000673 0.003631 0.008076 0.000327 0.002176 0.004092 0.007735 0.000587 0.001448
Mitotic Spindle 0.001573 0.006829 0.002179 0.008150 0.002057 0.009643 0.004596 0.037616 0.002044 0.008773 0.002380 0.023347
None 0.000375 0.002666 0.007190 0.006512 0.002793 0.002701 0.003136 0.006187 0.002728 0.002690 0.006853 0.006345
Nuclear Periphery 0.001402 0.001370 0.004188 0.002029 0.001722 0.000991 0.008417 0.002017 0.001713 0.001109 0.004539 0.002023
Nuclear Periphery Foci 0.004906 0.001587 0.002155 0.008395 0.001196 0.000798 0.009526 0.003715 0.001295 0.001042 0.002767 0.005981
Nucleolus 0.016792 0.001888 0.000697 0.000671 0.001128 0.001910 0.001029 0.003022 0.001544 0.001903 0.000724 0.001884
Nucleus 0.062210 0.085510 0.513477* 0.167330* 0.111394 0.109931 0.475609* 0.179700* 0.110086 0.102377 0.510334* 0.173710*
Peroxisomes 0.006483 0.011472 0.000169 0.001815 0.003208 0.007176 0.002202 0.001208 0.003295 0.008505 0.000338 0.001502
Vacuole 0.323116* 0.175043 0.028633 0.203675* 0.208611* 0.145585 0.175803 0.208685* 0.211656* 0.154698 0.040849 0.206259*
Vacuole Periphery 0.009695 0.004880 0.000128 0.001157 0.002208 0.003176 0.000306 0.005549 0.002407 0.003703 0.000142 0.003422

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.22 1.47 1.12 -1.15 -4.82 -4.05 -3.56 -4.23 -3.57 -3.03 -3.64 -0.38 -5.00 -3.89 -4.99
Bud Neck -0.92 1.57 1.58 8.43 0.03 -8.72 7.24 -10.17 -5.30 -13.40 -8.84 5.97 -7.03 -0.09 -11.85
Bud Site 2.34 2.71 2.31 -0.33 -4.55 -9.03 -0.61 -12.80 -7.35 -9.60 -7.24 -0.19 -10.88 -5.59 -10.94
Cell Periphery 1.85 1.87 1.71 -2.39 -2.71 5.55 -1.04 -5.90 -8.15 -5.20 6.32 5.92 -3.44 -8.31 -8.04
Cytoplasm -3.24 -3.91 -5.08 -7.18 -5.53 -3.62 13.13 4.72 8.37 -9.66 -1.72 2.77 -0.00 1.91 -3.06
Cytoplasmic Foci -7.31 4.67 3.48 32.66 -13.07 -1.32 19.50 31.75 38.93 4.75 -4.86 35.64 31.82 50.11 -11.56
Eisosomes -3.01 3.49 1.21 6.51 -6.82 4.42 2.03 8.54 7.12 8.75 4.42 10.88 8.98 9.76 -7.48
Endoplasmic Reticulum 1.36 1.85 1.26 -0.50 -3.91 5.77 4.54 5.64 0.06 1.30 5.74 7.19 5.38 -0.66 -3.76
Endosome 2.56 4.10 3.82 15.43 -9.99 0.25 10.59 14.60 15.78 2.36 -0.63 19.34 14.76 21.67 -12.09
Golgi 0.63 2.63 2.49 6.08 -5.62 -3.32 6.67 -0.45 1.43 -3.01 -3.73 7.85 1.62 4.66 -3.56
Lipid Particles -0.82 3.76 2.21 7.38 -7.50 6.55 7.36 13.84 13.08 5.95 6.61 15.01 13.25 14.04 -6.75
Mitochondria 0.79 1.14 1.15 5.69 0.63 -4.32 4.64 2.11 7.72 -6.53 -3.48 4.15 3.25 10.12 -5.07
Mitotic Spindle -2.83 -0.66 -3.35 -0.20 -3.14 -4.78 -0.50 -9.73 -7.09 -8.26 -5.02 -0.43 -9.59 -5.90 -9.94
None -2.94 -8.75 -6.51 -2.37 1.59 0.11 -0.01 -3.14 -4.27 -3.64 0.07 -3.57 -3.05 -4.60 1.13
Nuclear Periphery 0.02 -3.12 -0.98 -3.42 4.25 3.37 -2.05 -3.15 -7.89 1.74 2.94 -5.65 -3.04 -8.24 4.56
Nuclear Periphery Foci 1.67 1.43 -0.45 -5.40 -4.81 1.82 -4.72 -4.87 -6.00 3.57 1.29 -4.02 -6.82 -7.42 -3.78
Nucleolus 1.45 1.57 1.58 6.50 1.10 -2.72 1.04 -5.54 -4.71 -5.69 -1.04 2.66 -3.86 -3.51 -5.73
Nucleus -0.87 -17.00 -5.04 -15.88 39.49 0.27 -17.66 -15.61 -16.58 11.36 1.65 -62.53 -18.35 -22.39 43.39
Peroxisomes -1.57 2.75 2.31 9.11 -3.03 -7.33 2.03 4.41 10.56 0.66 -9.50 9.84 5.46 14.13 -3.67
Vacuole 3.27 6.43 1.54 -8.17 -28.91 8.95 -1.02 -2.07 -9.96 -0.15 8.36 25.35 -3.76 -14.00 -33.34
Vacuole Periphery 2.08 4.20 3.91 5.92 -4.92 -1.84 4.54 -0.88 0.29 -3.78 -2.45 5.42 0.93 3.79 -4.76
Standard name
Human Ortholog
Description Endosomal protein involved in turnover of plasma membrane proteins; member of the DUP380 subfamily of conserved, often subtelomeric COS genes; required for the multivesicular vesicle body sorting pathway that internalizes plasma membrane proteins for degradation; Cos proteins provide ubiquitin in trans for nonubiquitinated cargo proteins
Localization
Cell Percentages vacuole (25%), cytoplasm (7%), mixed (35%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Cos10

Cos10


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cos10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available