Standard name
Human Ortholog
Description Component of both the Rpd3S and Rpd3L histone deacetylase complexes; involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.96 0.95 0.98 0.97 0.94 0.92 0.87 0.84 0.86 0.73 0.74 1.0 0.99 0.98 – 0.91 0.92 0.74 0.81 0.61 0.87 0.81 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.1 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0.08 0.08 0 0.15 0.16 0.2 0.28 0.32 0.41 0.43 0 0 0 – 0 0 0.14 0.08 0.21 0 0.07 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 2 3 4 0 2 1
Bud 0 0 3 0 0 2 5 10 5 9 11 7 0 0 3 – 1 0 0 1 2 0 4 4
Bud Neck 1 0 2 1 5 7 7 8 1 4 4 3 0 0 0 – 1 1 1 0 0 0 1 1
Bud Site 0 0 0 0 0 0 2 1 1 0 1 3 0 0 0 – 0 0 – – – – – –
Cell Periphery 9 3 1 0 1 1 3 4 2 2 0 3 0 0 3 – 2 1 2 1 4 0 1 0
Cytoplasm 223 147 152 191 307 297 364 448 197 261 183 227 128 271 310 – 74 103 221 123 92 97 166 194
Endoplasmic Reticulum 0 1 0 0 1 2 0 0 0 0 0 0 0 0 1 – 2 11 4 0 0 1 0 1
Endosome 1 0 0 0 0 0 2 0 0 0 0 0 0 0 0 – 5 3 2 1 1 2 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 1 1 1 0 0 0 1 0
Mitochondria 2 8 13 15 11 49 64 101 65 98 102 130 0 1 6 – 1 1 42 11 32 3 13 8
Nucleus 1 0 1 0 0 0 2 3 1 0 3 0 0 1 0 – 0 0 0 0 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 – 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 1 0 1 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 1 0 1 0 0 0
SpindlePole 2 0 0 1 1 0 5 8 9 9 6 11 0 0 0 – 3 1 3 2 1 0 2 2
Vac/Vac Membrane 1 0 0 0 0 0 2 2 0 1 1 2 0 0 1 – 3 3 7 2 2 0 4 3
Unique Cell Count 232 153 160 194 315 317 396 513 235 305 251 305 128 273 315 81 112 299 152 152 112 206 226
Labelled Cell Count 240 159 172 208 326 358 457 586 281 384 311 388 128 274 324 93 125 299 152 152 112 206 226


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 3.2 2.9 2.8 3.2 2.9 3.0 2.9 2.7 2.3 2.9 2.2 3.9 4.1 3.5 4.9 5.3 6.2 3.7 3.7 4.3
Std Deviation (1e-4) 0.8 1.2 1.1 1.2 1.4 1.4 2.1 1.3 1.9 0.8 2.2 1.2 1.5 1.2 1.1 1.4 1.7 3.0 1.3 1.5 1.8
Intensity Change (Log2) – – – -0.06 0.14 -0.02 0.05 -0.01 -0.1 -0.33 0.02 -0.38 0.42 0.5 0.3 0.75 0.88 1.09 0.34 0.38 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.1855 0.1569 -0.0332 -0.0331 -0.4548 0.5301 0.0377 0.0619 0.0041 -0.2709 0.2222 0.3585 0.0693 0.1232 -0.4755 -0.0139 -1.2523 -0.2179
Actin 0.0112 0.0001 0.0036 0.0002 0.0001 0.0174 0.0277 0.0002 0.0073 0.001 0.0054 0.0135 0.0489 0.0042 0.0001 0.0001 0.0002 0.0001
Bud 0.0004 0 0.0009 0 0 0.0003 0.0054 0.0002 0.0049 0.0004 0.0004 0.0002 0.0006 0.0002 0 0 0 0
Bud Neck 0.0006 0 0.0003 0 0 0.0006 0.0038 0 0.0021 0 0.0011 0.0005 0.0005 0.0173 0 0 0 0.0001
Bud Periphery 0.0011 0 0.0017 0 0 0.0007 0.0025 0.0004 0.0024 0.0004 0.0004 0.0002 0.0016 0.0005 0 0.0002 0.0001 0
Bud Site 0.0053 0.0001 0.0031 0 0 0.0004 0.0219 0.0007 0.0112 0.0002 0.0012 0.0011 0.0049 0.0104 0 0 0 0
Cell Periphery 0.0007 0.0001 0.0003 0 0 0.0002 0.0004 0.0002 0.0002 0 0.0001 0 0.0004 0.0017 0 0 0.0003 0
Cytoplasm 0.0683 0.0833 0.073 0.0966 0.0444 0.1291 0.0551 0.1178 0.0932 0.0598 0.094 0.1422 0.0019 0.0098 0.0072 0.0095 0.0093 0.0322
Cytoplasmic Foci 0.0116 0.003 0.0082 0.003 0.0018 0.0097 0.03 0.0026 0.0083 0.0309 0.0582 0.0144 0.0027 0.0021 0.0005 0.0012 0.0005 0.001
Eisosomes 0.0006 0.0002 0.0002 0.0001 0.0001 0.0002 0.0005 0.0001 0.0002 0 0.0001 0.0001 0.0043 0.0039 0.0002 0.0001 0.001 0.0002
Endoplasmic Reticulum 0.0027 0.0008 0.0017 0.0007 0.0005 0.0015 0.0032 0.0008 0.0006 0.0012 0.0007 0.0005 0.0004 0.0003 0.0001 0.0001 0.0002 0.0001
Endosome 0.0175 0.0017 0.0047 0.0006 0.0002 0.0088 0.0331 0.0003 0.0072 0.0204 0.0152 0.0036 0.0021 0.0001 0 0 0 0.0001
Golgi 0.0064 0.0001 0.0021 0 0 0.0085 0.0136 0.0001 0.0032 0.0056 0.0075 0.0046 0.0033 0.0002 0 0 0 0
Lipid Particles 0.0172 0.0002 0.0054 0.0003 0.0001 0.0076 0.0372 0.0002 0.0039 0.0019 0.0279 0.0021 0.0167 0.0002 0.0001 0.0001 0.0001 0
Mitochondria 0.0108 0.0001 0.0101 0.0002 0 0.0069 0.0299 0.0002 0.0008 0.0071 0.0232 0.0008 0.2047 0.0004 0 0.0001 0.0001 0
None 0.796 0.9092 0.8667 0.897 0.9523 0.7733 0.6901 0.8747 0.8443 0.8616 0.7081 0.8105 0.6648 0.9473 0.9914 0.9877 0.9878 0.9658
Nuclear Periphery 0.0153 0.0001 0.0072 0.0002 0.0001 0.0193 0.0081 0.0002 0.0003 0.0011 0.0006 0.0003 0.0003 0.0001 0 0.0001 0 0
Nucleolus 0.0008 0.0001 0.0015 0 0 0.0004 0.001 0.0001 0.0003 0.0014 0.0013 0.0002 0.0004 0.0003 0 0.0001 0.0001 0
Nucleus 0.0191 0.0003 0.0014 0.0002 0.0001 0.0044 0.0009 0.0003 0.0004 0.0005 0.0008 0.0005 0.0002 0.0003 0 0.0001 0 0.0001
Peroxisomes 0.008 0 0.0041 0.0003 0 0.0057 0.0157 0.0001 0.0053 0.0004 0.0482 0.0014 0.0397 0.0004 0 0.0002 0.0001 0
Punctate Nuclear 0.0021 0.0002 0.0025 0.0002 0.0002 0.0014 0.0098 0.0003 0.0015 0.004 0.0031 0.0028 0.0003 0.0002 0.0001 0.0002 0 0
Vacuole 0.0026 0.0003 0.0008 0.0001 0.0001 0.0017 0.0082 0.0004 0.0022 0.0008 0.0021 0.0003 0.0006 0.0001 0 0.0001 0.0001 0.0001
Vacuole Periphery 0.0017 0 0.0008 0 0 0.0019 0.0017 0 0.0003 0.0011 0.0005 0.0001 0.0006 0 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 67.0035 48.1316 53.018 68.936 58.0188 56.4431 64.5633 65.2543 61.7235 72.3898
Translational Efficiency 1.0435 1.2087 0.9666 0.7529 0.972 1.1207 0.7378 0.655 0.7916 0.7387

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Component of both the Rpd3S and Rpd3L histone deacetylase complexes; involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Sin3

Sin3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sin3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available