Standard name
Human Ortholog
Description Topoisomerase I; nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination; role in processing ribonucleoside monophosphates in genomic DNA into irreversible single-strand breaks; enzymatic activity and interaction with Nsr1p are negatively regulated by polyphosphorylation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.08 0.05 0.08 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 0 0 0 0 0.08 0.11 0.12 0 0.06 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.05 0 0.06 0.05 0.12 0.07 0.05 0 0 0 0 0 0.1 0.08 0.07 0.09 0.06 0 0 0.07 0.1 0.1 0.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.09 0 0 0.05 0.18 0.13 0.07 0.28 0.33 0.29 0.14 0.13 0.05 0.06 0.07 0.06 0.12 0 0 0 0 0 0
Nucleus 0.42 0.4 0.42 0.25 0.25 0.25 0.24 0.24 0.23 0.2 0.19 0.3 0.31 0.23 0.21 0.42 0.31 0.44 0.28 0.32 0.23 0.11 0.11 0.16
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.67 0.65 0.56 0.73 0.61 0.62 0.68 0.69 0.74 0.7 0.71 0.66 0.64 0.64 0.64 0.51 0.57 0.44 0.56 0.48 0.43 0.44 0.35 0.23
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0.09 0.29 0.29 0.37 0.24 0.19 0.29 0.18 0.16 0.2 0.2 0.09 0.16 0.2 0.16 0.15 0.15 0.1 0.1 0.2 0.19 0.24 0.2
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0.08 0.08 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 4 1 3 2 2 3 6 6 4 0 0 0 1 2 1 0 2 2 0 0 2
Bud Neck 0 0 0 2 0 0 2 3 5 2 7 2 1 0 1 2 0 0 0 2 1 1 3 4
Bud Site 0 0 0 3 3 0 10 9 17 12 22 15 3 1 0 2 2 2 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2 1 5 10 6 8 7 2 4 4 7 15 18 17 20 25 22 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 1 1 1 1 0 0 0 0 0 1 0 5 5 6 0 0 0 0 1 1
Endosome 3 11 9 9 9 26 18 12 1 2 7 9 4 16 11 34 37 24 3 9 18 12 21 18
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 2
Mitochondria 10 20 9 5 9 38 35 17 59 80 84 43 24 9 9 34 25 46 1 2 3 1 4 4
Nucleus 67 93 97 40 48 54 66 58 50 48 53 89 57 38 30 219 127 165 47 83 63 14 21 29
Nuclear Periphery 0 1 2 0 1 5 2 3 3 1 4 4 3 0 0 12 14 15 1 0 2 1 2 7
Nucleolus 107 149 130 117 115 135 185 164 159 170 203 198 119 107 94 268 230 165 93 124 117 58 73 42
Peroxisomes 0 0 0 0 0 2 3 0 2 0 1 0 0 1 3 0 2 1 0 0 0 0 2 3
SpindlePole 6 21 68 47 69 51 52 69 39 38 58 59 16 26 29 83 62 56 15 26 53 25 50 36
Vac/Vac Membrane 1 3 4 1 0 0 0 0 0 1 0 4 3 1 5 16 20 18 1 5 6 10 17 24
Unique Cell Count 159 230 233 161 189 217 271 238 214 244 286 299 185 167 146 522 405 371 167 259 273 133 208 189
Labelled Cell Count 194 300 320 233 266 321 384 345 340 364 449 434 245 218 199 697 551 521 167 259 273 133 208 189


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 7.0 6.9 6.7 7.6 5.9 6.3 6.1 5.2 4.9 5.0 5.8 7.1 7.5 7.2 9.1 10.5 8.7 6.7 6.9 6.9
Std Deviation (1e-4) 1.1 1.5 1.6 1.7 2.3 1.7 1.6 1.6 1.3 1.2 1.3 1.5 2.2 2.2 2.6 2.8 3.1 2.8 1.6 1.8 1.7
Intensity Change (Log2) – – – -0.04 0.14 -0.23 -0.13 -0.18 -0.41 -0.49 -0.46 -0.26 0.04 0.13 0.07 0.4 0.6 0.34 -0.04 0.0 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 4.4 3.4 4.3 3.5 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 4.1 4.8 5.0 2.6 3.5 3.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.8 0.5 3.2 1.4 0.6 0 -2.2 -0.9 -0.5 -1.0 2.3 1.6 1.5 2.5 1.4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.4 0.5 4.7 3.6 1.6 7.0 8.1 7.5 4.1 3.4 0.7 1.0 1.5 1.3 3.5
Nucleus -3.4 -3.5 -3.8 -4.1 -4.0 -4.1 -5.2 -5.8 -2.8 -2.3 -3.9 -4.2 0.1 -2.6 0.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 2.0 2.3
Nucleolus 3.4 1.0 1.4 2.9 2.9 4.1 3.1 3.6 2.5 1.8 1.7 1.7 -1.1 0.2 -2.7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1.6 -1.4 -2.6 0 -2.7 -3.6 -2.4 -2.5 -5.2 -3.2 -2.0 -4.2 -4.2 -4.2
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 1.1 2.1 2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.3637 6.2664 6.0713 4.9828 5.258 5.9918 9.7903 18.6943 8.5981 7.4412 6.8465 7.7922 8.3702 6.9471 7.1107 6.4064 6.4936 6.808
Actin 0 0 0 0 0.0001 0 0 0 0.0001 0 0.0001 0.0001 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.003 0 0.002 0 0 0.0006 0 0 0 0 0 0
Bud Neck 0.0001 0.0001 0.0001 0.0001 0.0003 0.0003 0.0001 0.0002 0.0002 0.0002 0.0007 0.0004 0.0001 0.0001 0.0001 0.0001 0.0004 0.0003
Bud Periphery 0 0 0 0 0.0001 0.0001 0.0012 0.0001 0.0004 0 0.0001 0.0002 0.0001 0 0 0 0 0
Bud Site 0.0001 0 0 0 0.0002 0.0001 0.0008 0.0001 0.0009 0 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0001 0
Cell Periphery 0 0 0 0 0 0 0.0003 0.0001 0.0002 0 0.0001 0.0001 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.0025 0.0145 0.0076 0 0 0.0088 0 0 0 0 0 0
Cytoplasmic Foci 0 0 0 0.0001 0.0005 0.0001 0.006 0 0.007 0.0001 0.0003 0.003 0.0001 0 0.0002 0 0.0001 0
Eisosomes 0 0 0 0 0 0 0.0001 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0.0001 0 0 0.0004 0 0 0 0 0 0
Endosome 0 0.0003 0 0 0.0002 0.0001 0 0.0001 0 0 0.0002 0.0001 0 0 0 0 0 0
Golgi 0 0 0 0 0.0002 0 0 0 0 0 0.0002 0 0 0 0 0 0 0
Lipid Particles 0 0.0001 0.0001 0 0.0018 0.0002 0.0009 0 0.0003 0.0001 0.0043 0.0005 0.0001 0 0.0008 0.0001 0.0002 0.0001
Mitochondria 0.0002 0.0001 0 0 0.0003 0.0001 0.0007 0.0001 0.0021 0.0001 0.0005 0.0006 0.0002 0 0.0001 0 0.0001 0
None 0 0 0 0 0.0001 0.0005 0.002 0 0.0049 0 0.0001 0.0008 0 0 0 0 0 0
Nuclear Periphery 0.0004 0.0023 0.0008 0.0001 0.0041 0.0009 0.0008 0.0041 0.0012 0.0054 0.0076 0.0012 0.0006 0.0008 0.0009 0.0007 0.0024 0.0001
Nucleolus 0.5292 0.4451 0.4982 0.504 0.6251 0.6209 0.6996 0.5304 0.6446 0.6042 0.7232 0.6907 0.6135 0.4229 0.5858 0.6517 0.673 0.6881
Nucleus 0.4642 0.543 0.494 0.4082 0.2859 0.3631 0.2654 0.4377 0.3089 0.3797 0.2232 0.2739 0.3674 0.5489 0.392 0.3229 0.2642 0.2938
Peroxisomes 0 0 0 0 0.0002 0 0.0001 0 0.0001 0 0.0002 0 0 0 0 0 0 0
Punctate Nuclear 0.0055 0.0057 0.0065 0.0872 0.0802 0.0135 0.0157 0.0111 0.0188 0.0099 0.0384 0.0181 0.0172 0.0273 0.0196 0.0244 0.0594 0.0175
Vacuole 0.0001 0.0021 0 0 0.0002 0.0001 0.0006 0.001 0.0004 0.0001 0.0002 0.0002 0.0001 0 0.0001 0 0 0
Vacuole Periphery 0.0001 0.0009 0 0 0.0002 0 0.0001 0.0005 0.0002 0.0001 0.0004 0.0001 0.0001 0 0.0001 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 36.5413 25.6202 26.9326 42.1844 47.3774 37.2372 21.803 21.8548 38.7872 43.0445
Translational Efficiency 1.4994 1.3522 1.2528 1.4139 1.2216 1.3759 1.1397 0.9283 0.9447 1.1524

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2104 123 1555 703 1634 2130 1852 1108 3738 2253 3407 1811

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 761.86 958.88 1125.53 1172.98 779.34 890.18 1112.77 1155.63 769.50 893.93 1118.59 1162.36
Standard Deviation 93.99 154.39 188.52 175.45 140.68 144.48 171.84 220.02 117.04 145.87 179.75 204.05
Intensity Change Log 2 — 0.331824 0.563007 0.622581 — 0.191844 0.513831 0.568355 — 0.262738 0.538349 0.595415

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000171 0.000784 0.000424 0.000487 0.000275 0.000382 0.000350 0.000474 0.000217 0.000404 0.000384 0.000479
Bud Neck 0.004481 0.003200 0.006741 0.011703 0.002893 0.005650 0.007635 0.006822 0.003787 0.005516 0.007227 0.008717
Bud Site 0.001520 0.000730 0.000655 0.001470 0.001201 0.001188 0.000700 0.001570 0.001381 0.001163 0.000679 0.001531
Cell Periphery 0.000108 0.000049 0.000017 0.000033 0.000091 0.000088 0.000019 0.000036 0.000101 0.000086 0.000018 0.000035
Cytoplasm 0.003179 0.000303 0.000087 0.000343 0.002618 0.001675 0.000220 0.000262 0.002934 0.001600 0.000159 0.000294
Cytoplasmic Foci 0.001926 0.000601 0.000064 0.000245 0.001428 0.001647 0.000051 0.000827 0.001708 0.001590 0.000057 0.000601
Eisosomes 0.000088 0.000082 0.000044 0.000051 0.000064 0.000054 0.000045 0.000046 0.000077 0.000055 0.000045 0.000048
Endoplasmic Reticulum 0.001416 0.003298 0.000588 0.000818 0.001728 0.001801 0.000594 0.000579 0.001552 0.001883 0.000591 0.000672
Endosome 0.001624 0.000367 0.000056 0.000342 0.001354 0.002229 0.000095 0.000488 0.001506 0.002128 0.000077 0.000432
Golgi 0.002002 0.001301 0.000319 0.000394 0.001637 0.001304 0.000296 0.000453 0.001843 0.001304 0.000306 0.000430
Lipid Particles 0.000764 0.000642 0.000019 0.000045 0.000498 0.000465 0.000017 0.000062 0.000648 0.000474 0.000018 0.000056
Mitochondria 0.000984 0.000653 0.000827 0.001587 0.000869 0.003573 0.000801 0.001370 0.000934 0.003413 0.000813 0.001455
Mitotic Spindle 0.038044 0.031202 0.021864 0.033604 0.038023 0.023006 0.015120 0.010801 0.038035 0.023453 0.018198 0.019652
None 0.004819 0.000901 0.000648 0.001021 0.011703 0.002794 0.000628 0.000863 0.007828 0.002690 0.000637 0.000924
Nuclear Periphery 0.000531 0.000262 0.000157 0.000536 0.000451 0.000715 0.000312 0.000333 0.000496 0.000690 0.000241 0.000412
Nuclear Periphery Foci 0.029024 0.011218 0.000389 0.000899 0.019471 0.006075 0.000468 0.000601 0.024848 0.006356 0.000432 0.000717
Nucleolus 0.672002* 0.532717* 0.537329* 0.543799* 0.580200* 0.522815* 0.563606* 0.533023* 0.631873* 0.523355* 0.551613* 0.537206*
Nucleus 0.232473* 0.400310* 0.423822* 0.394660* 0.330977* 0.419177* 0.405176* 0.433457* 0.275532* 0.418147* 0.413686* 0.418396*
Peroxisomes 0.004203 0.010531 0.005504 0.005804 0.003857 0.002710 0.003430 0.006019 0.004052 0.003137 0.004377 0.005935
Vacuole 0.000405 0.000614 0.000214 0.001117 0.000452 0.001669 0.000205 0.001303 0.000426 0.001612 0.000209 0.001231
Vacuole Periphery 0.000237 0.000235 0.000232 0.001044 0.000209 0.000984 0.000230 0.000611 0.000225 0.000943 0.000231 0.000779

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.40 -14.13 -13.11 0.71 -4.57 -1.57 -1.77 -4.04 -3.59 -4.53 -5.27 -8.78 -12.11 -4.52 -6.08
Bud Neck 1.80 -6.94 -9.79 -10.10 -6.66 -5.83 -10.19 -9.19 -4.02 1.12 -4.25 -11.88 -12.77 -8.51 -4.10
Bud Site 2.61 2.64 -3.10 -6.09 -6.36 0.53 1.59 -0.62 -0.89 -1.31 1.52 3.06 -1.60 -2.52 -3.25
Cell Periphery 2.30 4.98 3.41 0.51 -5.87 0.19 5.38 3.44 2.86 -1.96 0.74 7.02 4.69 2.94 -3.68
Cytoplasm 4.41 4.84 4.11 -1.13 -3.33 1.70 4.12 3.06 2.07 -0.94 2.46 6.28 4.75 2.65 -1.57
Cytoplasmic Foci 4.14 5.93 4.50 0.24 -3.85 0.66 3.47 1.80 1.41 -1.95 1.58 6.61 3.85 1.82 -2.72
Eisosomes 0.89 19.42 12.53 3.22 -4.04 5.01 10.34 9.21 4.32 -0.21 11.82 21.86 17.04 3.55 -2.92
Endoplasmic Reticulum -2.75 11.92 2.78 3.20 -3.71 0.04 11.61 11.26 10.64 0.76 -2.30 16.82 10.83 9.23 -2.49
Endosome 5.31 7.23 5.09 -0.12 -2.84 -1.40 4.81 2.78 3.50 -1.74 -0.95 8.57 5.21 3.72 -2.63
Golgi 2.26 8.76 7.71 2.71 -6.42 1.36 4.07 3.43 3.39 -0.90 3.28 8.75 7.26 3.87 -1.83
Lipid Particles 0.06 3.43 3.16 1.95 -3.92 0.49 5.62 4.89 3.76 -1.48 1.21 4.97 4.55 4.06 -2.59
Mitochondria 2.09 0.17 -4.29 -5.56 -4.77 -3.50 -0.47 -3.69 2.69 -3.91 -3.45 -0.19 -5.55 2.44 -6.11
Mitotic Spindle 0.35 5.29 -0.08 -0.37 -3.72 4.18 6.41 5.54 2.19 -0.24 6.21 8.61 4.40 -1.06 -2.75
None 6.60 7.27 6.42 -0.76 -2.80 5.24 6.68 6.50 4.10 -1.95 5.92 9.11 8.64 3.99 -3.19
Nuclear Periphery 4.39 7.41 -1.34 -3.67 -4.70 -2.31 1.37 0.87 2.51 -0.40 -1.93 4.09 0.09 1.72 -2.74
Nuclear Periphery Foci 7.07 17.31 16.75 4.10 -2.69 7.74 11.48 11.32 8.65 -0.68 14.77 20.70 20.26 9.09 -2.21
Nucleolus 6.51 14.74 11.24 -0.04 1.11 6.55 1.76 4.74 -0.19 3.27 15.34* 12.29 12.45 -0.27 3.14
Nucleus -8.32 -25.21 -14.03 0.29 2.40 -11.84 -9.75 -10.26 -1.55 -2.53 -23.82 -25.46 -19.10 0.16 -0.51
Peroxisomes -2.39 -2.05 -3.88 1.20 -2.84 3.40 2.17 -3.40 -8.15 -7.21 3.67 0.38 -5.01 -8.46 -6.27
Vacuole -1.67 0.87 -3.57 -2.72 -3.83 -3.63 2.38 -1.55 0.28 -1.90 -3.87 2.34 -2.64 0.19 -2.99
Vacuole Periphery -0.46 -1.07 -2.16 -2.10 -2.08 -3.90 -1.87 -3.16 1.56 -2.86 -3.87 -1.92 -3.48 0.85 -3.27
Standard name
Human Ortholog
Description Topoisomerase I; nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination; role in processing ribonucleoside monophosphates in genomic DNA into irreversible single-strand breaks; enzymatic activity and interaction with Nsr1p are negatively regulated by polyphosphorylation
Localization
Cell Percentages nucleolus (56%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-1

Top1

Top1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Top1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available