Standard name
Human Ortholog
Description Topoisomerase I; nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination; role in processing ribonucleoside monophosphates in genomic DNA into irreversible single-strand breaks; enzymatic activity and interaction with Nsr1p are negatively regulated by polyphosphorylation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.08 0.05 0.08 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 0 0 0 0 0.08 0.11 0.12 0 0.06 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.05 0 0.06 0.05 0.12 0.07 0.05 0 0 0 0 0 0.1 0.08 0.07 0.09 0.06 0 0 0.07 0.1 0.1 0.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.09 0 0 0.05 0.18 0.13 0.07 0.28 0.33 0.29 0.14 0.13 0.05 0.06 0.07 0.06 0.12 0 0 0 0 0 0
Nucleus 0.42 0.4 0.42 0.25 0.25 0.25 0.24 0.24 0.23 0.2 0.19 0.3 0.31 0.23 0.21 0.42 0.31 0.44 0.28 0.32 0.23 0.11 0.11 0.16
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.67 0.65 0.56 0.73 0.61 0.62 0.68 0.69 0.74 0.7 0.71 0.66 0.64 0.64 0.64 0.51 0.57 0.44 0.56 0.48 0.43 0.44 0.35 0.23
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0.09 0.29 0.29 0.37 0.24 0.19 0.29 0.18 0.16 0.2 0.2 0.09 0.16 0.2 0.16 0.15 0.15 0.1 0.1 0.2 0.19 0.24 0.2
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0.08 0.08 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 4 1 3 2 2 3 6 6 4 0 0 0 1 2 1 0 2 2 0 0 2
Bud Neck 0 0 0 2 0 0 2 3 5 2 7 2 1 0 1 2 0 0 0 2 1 1 3 4
Bud Site 0 0 0 3 3 0 10 9 17 12 22 15 3 1 0 2 2 2 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2 1 5 10 6 8 7 2 4 4 7 15 18 17 20 25 22 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 1 1 1 1 0 0 0 0 0 1 0 5 5 6 0 0 0 0 1 1
Endosome 3 11 9 9 9 26 18 12 1 2 7 9 4 16 11 34 37 24 3 9 18 12 21 18
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 2
Mitochondria 10 20 9 5 9 38 35 17 59 80 84 43 24 9 9 34 25 46 1 2 3 1 4 4
Nucleus 67 93 97 40 48 54 66 58 50 48 53 89 57 38 30 219 127 165 47 83 63 14 21 29
Nuclear Periphery 0 1 2 0 1 5 2 3 3 1 4 4 3 0 0 12 14 15 1 0 2 1 2 7
Nucleolus 107 149 130 117 115 135 185 164 159 170 203 198 119 107 94 268 230 165 93 124 117 58 73 42
Peroxisomes 0 0 0 0 0 2 3 0 2 0 1 0 0 1 3 0 2 1 0 0 0 0 2 3
SpindlePole 6 21 68 47 69 51 52 69 39 38 58 59 16 26 29 83 62 56 15 26 53 25 50 36
Vac/Vac Membrane 1 3 4 1 0 0 0 0 0 1 0 4 3 1 5 16 20 18 1 5 6 10 17 24
Unique Cell Count 159 230 233 161 189 217 271 238 214 244 286 299 185 167 146 522 405 371 167 259 273 133 208 189
Labelled Cell Count 194 300 320 233 266 321 384 345 340 364 449 434 245 218 199 697 551 521 167 259 273 133 208 189


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 7.0 6.9 6.7 7.6 5.9 6.3 6.1 5.2 4.9 5.0 5.8 7.1 7.5 7.2 9.1 10.5 8.7 6.7 6.9 6.9
Std Deviation (1e-4) 1.1 1.5 1.6 1.7 2.3 1.7 1.6 1.6 1.3 1.2 1.3 1.5 2.2 2.2 2.6 2.8 3.1 2.8 1.6 1.8 1.7
Intensity Change (Log2) – – – -0.04 0.14 -0.23 -0.13 -0.18 -0.41 -0.49 -0.46 -0.26 0.04 0.13 0.07 0.4 0.6 0.34 -0.04 0.0 0.01

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 4.4 3.4 4.3 3.5 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 4.1 4.8 5.0 2.6 3.5 3.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.8 0.5 3.2 1.4 0.6 0 -2.2 -0.9 -0.5 -1.0 2.3 1.6 1.5 2.5 1.4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.4 0.5 4.7 3.6 1.6 7.0 8.1 7.5 4.1 3.4 0.7 1.0 1.5 1.3 3.5
Nucleus -3.4 -3.5 -3.8 -4.1 -4.0 -4.1 -5.2 -5.8 -2.8 -2.3 -3.9 -4.2 0.1 -2.6 0.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 2.0 2.3
Nucleolus 3.4 1.0 1.4 2.9 2.9 4.1 3.1 3.6 2.5 1.8 1.7 1.7 -1.1 0.2 -2.7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1.6 -1.4 -2.6 0 -2.7 -3.6 -2.4 -2.5 -5.2 -3.2 -2.0 -4.2 -4.2 -4.2
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 1.1 2.1 2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.3637 6.2664 6.0713 4.9828 5.258 5.9918 9.7903 18.6943 8.5981 7.4412 6.8465 7.7922 8.3702 6.9471 7.1107 6.4064 6.4936 6.808
Actin 0 0 0 0 0.0001 0 0 0 0.0001 0 0.0001 0.0001 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.003 0 0.002 0 0 0.0006 0 0 0 0 0 0
Bud Neck 0.0001 0.0001 0.0001 0.0001 0.0003 0.0003 0.0001 0.0002 0.0002 0.0002 0.0007 0.0004 0.0001 0.0001 0.0001 0.0001 0.0004 0.0003
Bud Periphery 0 0 0 0 0.0001 0.0001 0.0012 0.0001 0.0004 0 0.0001 0.0002 0.0001 0 0 0 0 0
Bud Site 0.0001 0 0 0 0.0002 0.0001 0.0008 0.0001 0.0009 0 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0001 0
Cell Periphery 0 0 0 0 0 0 0.0003 0.0001 0.0002 0 0.0001 0.0001 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.0025 0.0145 0.0076 0 0 0.0088 0 0 0 0 0 0
Cytoplasmic Foci 0 0 0 0.0001 0.0005 0.0001 0.006 0 0.007 0.0001 0.0003 0.003 0.0001 0 0.0002 0 0.0001 0
Eisosomes 0 0 0 0 0 0 0.0001 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0.0001 0 0 0.0004 0 0 0 0 0 0
Endosome 0 0.0003 0 0 0.0002 0.0001 0 0.0001 0 0 0.0002 0.0001 0 0 0 0 0 0
Golgi 0 0 0 0 0.0002 0 0 0 0 0 0.0002 0 0 0 0 0 0 0
Lipid Particles 0 0.0001 0.0001 0 0.0018 0.0002 0.0009 0 0.0003 0.0001 0.0043 0.0005 0.0001 0 0.0008 0.0001 0.0002 0.0001
Mitochondria 0.0002 0.0001 0 0 0.0003 0.0001 0.0007 0.0001 0.0021 0.0001 0.0005 0.0006 0.0002 0 0.0001 0 0.0001 0
None 0 0 0 0 0.0001 0.0005 0.002 0 0.0049 0 0.0001 0.0008 0 0 0 0 0 0
Nuclear Periphery 0.0004 0.0023 0.0008 0.0001 0.0041 0.0009 0.0008 0.0041 0.0012 0.0054 0.0076 0.0012 0.0006 0.0008 0.0009 0.0007 0.0024 0.0001
Nucleolus 0.5292 0.4451 0.4982 0.504 0.6251 0.6209 0.6996 0.5304 0.6446 0.6042 0.7232 0.6907 0.6135 0.4229 0.5858 0.6517 0.673 0.6881
Nucleus 0.4642 0.543 0.494 0.4082 0.2859 0.3631 0.2654 0.4377 0.3089 0.3797 0.2232 0.2739 0.3674 0.5489 0.392 0.3229 0.2642 0.2938
Peroxisomes 0 0 0 0 0.0002 0 0.0001 0 0.0001 0 0.0002 0 0 0 0 0 0 0
Punctate Nuclear 0.0055 0.0057 0.0065 0.0872 0.0802 0.0135 0.0157 0.0111 0.0188 0.0099 0.0384 0.0181 0.0172 0.0273 0.0196 0.0244 0.0594 0.0175
Vacuole 0.0001 0.0021 0 0 0.0002 0.0001 0.0006 0.001 0.0004 0.0001 0.0002 0.0002 0.0001 0 0.0001 0 0 0
Vacuole Periphery 0.0001 0.0009 0 0 0.0002 0 0.0001 0.0005 0.0002 0.0001 0.0004 0.0001 0.0001 0 0.0001 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 36.5413 25.6202 26.9326 42.1844 47.3774 37.2372 21.803 21.8548 38.7872 43.0445
Translational Efficiency 1.4994 1.3522 1.2528 1.4139 1.2216 1.3759 1.1397 0.9283 0.9447 1.1524

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2104 123 1555 703 1634 2130 1852 1108 3738 2253 3407 1811

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 761.86 958.88 1125.53 1172.98 779.34 890.18 1112.77 1155.63 769.50 893.93 1118.59 1162.36
Standard Deviation 93.99 154.39 188.52 175.45 140.68 144.48 171.84 220.02 117.04 145.87 179.75 204.05
Intensity Change Log 2 — 0.331824 0.563007 0.622581 — 0.191844 0.513831 0.568355 — 0.262738 0.538349 0.595415

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000171 0.000784 0.000424 0.000487 0.000275 0.000382 0.000350 0.000474 0.000217 0.000404 0.000384 0.000479
Bud Neck 0.004481 0.003200 0.006741 0.011703 0.002893 0.005650 0.007635 0.006822 0.003787 0.005516 0.007227 0.008717
Bud Site 0.001520 0.000730 0.000655 0.001470 0.001201 0.001188 0.000700 0.001570 0.001381 0.001163 0.000679 0.001531
Cell Periphery 0.000108 0.000049 0.000017 0.000033 0.000091 0.000088 0.000019 0.000036 0.000101 0.000086 0.000018 0.000035
Cytoplasm 0.003179 0.000303 0.000087 0.000343 0.002618 0.001675 0.000220 0.000262 0.002934 0.001600 0.000159 0.000294
Cytoplasmic Foci 0.001926 0.000601 0.000064 0.000245 0.001428 0.001647 0.000051 0.000827 0.001708 0.001590 0.000057 0.000601
Eisosomes 0.000088 0.000082 0.000044 0.000051 0.000064 0.000054 0.000045 0.000046 0.000077 0.000055 0.000045 0.000048
Endoplasmic Reticulum 0.001416 0.003298 0.000588 0.000818 0.001728 0.001801 0.000594 0.000579 0.001552 0.001883 0.000591 0.000672
Endosome 0.001624 0.000367 0.000056 0.000342 0.001354 0.002229 0.000095 0.000488 0.001506 0.002128 0.000077 0.000432
Golgi 0.002002 0.001301 0.000319 0.000394 0.001637 0.001304 0.000296 0.000453 0.001843 0.001304 0.000306 0.000430
Lipid Particles 0.000764 0.000642 0.000019 0.000045 0.000498 0.000465 0.000017 0.000062 0.000648 0.000474 0.000018 0.000056
Mitochondria 0.000984 0.000653 0.000827 0.001587 0.000869 0.003573 0.000801 0.001370 0.000934 0.003413 0.000813 0.001455
Mitotic Spindle 0.038044 0.031202 0.021864 0.033604 0.038023 0.023006 0.015120 0.010801 0.038035 0.023453 0.018198 0.019652
None 0.004819 0.000901 0.000648 0.001021 0.011703 0.002794 0.000628 0.000863 0.007828 0.002690 0.000637 0.000924
Nuclear Periphery 0.000531 0.000262 0.000157 0.000536 0.000451 0.000715 0.000312 0.000333 0.000496 0.000690 0.000241 0.000412
Nuclear Periphery Foci 0.029024 0.011218 0.000389 0.000899 0.019471 0.006075 0.000468 0.000601 0.024848 0.006356 0.000432 0.000717
Nucleolus 0.672002 0.532717 0.537329 0.543799 0.580200 0.522815 0.563606 0.533023 0.631873 0.523355 0.551613 0.537206
Nucleus 0.232473 0.400310 0.423822 0.394660 0.330977 0.419177 0.405176 0.433457 0.275532 0.418147 0.413686 0.418396
Peroxisomes 0.004203 0.010531 0.005504 0.005804 0.003857 0.002710 0.003430 0.006019 0.004052 0.003137 0.004377 0.005935
Vacuole 0.000405 0.000614 0.000214 0.001117 0.000452 0.001669 0.000205 0.001303 0.000426 0.001612 0.000209 0.001231
Vacuole Periphery 0.000237 0.000235 0.000232 0.001044 0.000209 0.000984 0.000230 0.000611 0.000225 0.000943 0.000231 0.000779

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.40 -14.13 -13.11 0.71 -4.57 -1.57 -1.77 -4.04 -3.59 -4.53 -5.27 -8.78 -12.11 -4.52 -6.08
Bud Neck 1.80 -6.94 -9.79 -10.10 -6.66 -5.83 -10.19 -9.19 -4.02 1.12 -4.25 -11.88 -12.77 -8.51 -4.10
Bud Site 2.61 2.64 -3.10 -6.09 -6.36 0.53 1.59 -0.62 -0.89 -1.31 1.52 3.06 -1.60 -2.52 -3.25
Cell Periphery 2.30 4.98 3.41 0.51 -5.87 0.19 5.38 3.44 2.86 -1.96 0.74 7.02 4.69 2.94 -3.68
Cytoplasm 4.41 4.84 4.11 -1.13 -3.33 1.70 4.12 3.06 2.07 -0.94 2.46 6.28 4.75 2.65 -1.57
Cytoplasmic Foci 4.14 5.93 4.50 0.24 -3.85 0.66 3.47 1.80 1.41 -1.95 1.58 6.61 3.85 1.82 -2.72
Eisosomes 0.89 19.42 12.53 3.22 -4.04 5.01 10.34 9.21 4.32 -0.21 11.82 21.86 17.04 3.55 -2.92
Endoplasmic Reticulum -2.75 11.92 2.78 3.20 -3.71 0.04 11.61 11.26 10.64 0.76 -2.30 16.82 10.83 9.23 -2.49
Endosome 5.31 7.23 5.09 -0.12 -2.84 -1.40 4.81 2.78 3.50 -1.74 -0.95 8.57 5.21 3.72 -2.63
Golgi 2.26 8.76 7.71 2.71 -6.42 1.36 4.07 3.43 3.39 -0.90 3.28 8.75 7.26 3.87 -1.83
Lipid Particles 0.06 3.43 3.16 1.95 -3.92 0.49 5.62 4.89 3.76 -1.48 1.21 4.97 4.55 4.06 -2.59
Mitochondria 2.09 0.17 -4.29 -5.56 -4.77 -3.50 -0.47 -3.69 2.69 -3.91 -3.45 -0.19 -5.55 2.44 -6.11
Mitotic Spindle 0.35 5.29 -0.08 -0.37 -3.72 4.18 6.41 5.54 2.19 -0.24 6.21 8.61 4.40 -1.06 -2.75
None 6.60 7.27 6.42 -0.76 -2.80 5.24 6.68 6.50 4.10 -1.95 5.92 9.11 8.64 3.99 -3.19
Nuclear Periphery 4.39 7.41 -1.34 -3.67 -4.70 -2.31 1.37 0.87 2.51 -0.40 -1.93 4.09 0.09 1.72 -2.74
Nuclear Periphery Foci 7.07 17.31 16.75 4.10 -2.69 7.74 11.48 11.32 8.65 -0.68 14.77 20.70 20.26 9.09 -2.21
Nucleolus 6.51 14.74 11.24 -0.04 1.11 6.55 1.76 4.74 -0.19 3.27 15.34 12.29 12.45 -0.27 3.14
Nucleus -8.32 -25.21 -14.03 0.29 2.40 -11.84 -9.75 -10.26 -1.55 -2.53 -23.82 -25.46 -19.10 0.16 -0.51
Peroxisomes -2.39 -2.05 -3.88 1.20 -2.84 3.40 2.17 -3.40 -8.15 -7.21 3.67 0.38 -5.01 -8.46 -6.27
Vacuole -1.67 0.87 -3.57 -2.72 -3.83 -3.63 2.38 -1.55 0.28 -1.90 -3.87 2.34 -2.64 0.19 -2.99
Vacuole Periphery -0.46 -1.07 -2.16 -2.10 -2.08 -3.90 -1.87 -3.16 1.56 -2.86 -3.87 -1.92 -3.48 0.85 -3.27
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Topoisomerase I; nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination; role in processing ribonucleoside monophosphates in genomic DNA into irreversible single-strand breaks; enzymatic activity and interaction with Nsr1p are negatively regulated by polyphosphorylation
Localization
Cell Percentages nucleolus (56%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-1

Top1

Top1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Top1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available