Standard name | |
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Human Ortholog | |
Description | Topoisomerase I; nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination; role in processing ribonucleoside monophosphates in genomic DNA into irreversible single-strand breaks; enzymatic activity and interaction with Nsr1p are negatively regulated by polyphosphorylation |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 4 | 1 | 3 | 2 | 2 | 3 | 6 | 6 | 4 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 2 | 2 | 0 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 3 | 5 | 2 | 7 | 2 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 2 | 1 | 1 | 3 | 4 |
Bud Site | 0 | 0 | 0 | 3 | 3 | 0 | 10 | 9 | 17 | 12 | 22 | 15 | 3 | 1 | 0 | 2 | 2 | 2 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 2 | 1 | 5 | 10 | 6 | 8 | 7 | 2 | 4 | 4 | 7 | 15 | 18 | 17 | 20 | 25 | 22 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 5 | 6 | 0 | 0 | 0 | 0 | 1 | 1 |
Endosome | 3 | 11 | 9 | 9 | 9 | 26 | 18 | 12 | 1 | 2 | 7 | 9 | 4 | 16 | 11 | 34 | 37 | 24 | 3 | 9 | 18 | 12 | 21 | 18 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 |
Mitochondria | 10 | 20 | 9 | 5 | 9 | 38 | 35 | 17 | 59 | 80 | 84 | 43 | 24 | 9 | 9 | 34 | 25 | 46 | 1 | 2 | 3 | 1 | 4 | 4 |
Nucleus | 67 | 93 | 97 | 40 | 48 | 54 | 66 | 58 | 50 | 48 | 53 | 89 | 57 | 38 | 30 | 219 | 127 | 165 | 47 | 83 | 63 | 14 | 21 | 29 |
Nuclear Periphery | 0 | 1 | 2 | 0 | 1 | 5 | 2 | 3 | 3 | 1 | 4 | 4 | 3 | 0 | 0 | 12 | 14 | 15 | 1 | 0 | 2 | 1 | 2 | 7 |
Nucleolus | 107 | 149 | 130 | 117 | 115 | 135 | 185 | 164 | 159 | 170 | 203 | 198 | 119 | 107 | 94 | 268 | 230 | 165 | 93 | 124 | 117 | 58 | 73 | 42 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 2 | 0 | 1 | 0 | 0 | 1 | 3 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 2 | 3 |
SpindlePole | 6 | 21 | 68 | 47 | 69 | 51 | 52 | 69 | 39 | 38 | 58 | 59 | 16 | 26 | 29 | 83 | 62 | 56 | 15 | 26 | 53 | 25 | 50 | 36 |
Vac/Vac Membrane | 1 | 3 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 3 | 1 | 5 | 16 | 20 | 18 | 1 | 5 | 6 | 10 | 17 | 24 |
Unique Cell Count | 159 | 230 | 233 | 161 | 189 | 217 | 271 | 238 | 214 | 244 | 286 | 299 | 185 | 167 | 146 | 522 | 405 | 371 | 167 | 259 | 273 | 133 | 208 | 189 |
Labelled Cell Count | 194 | 300 | 320 | 233 | 266 | 321 | 384 | 345 | 340 | 364 | 449 | 434 | 245 | 218 | 199 | 697 | 551 | 521 | 167 | 259 | 273 | 133 | 208 | 189 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.2 | 7.0 | 6.9 | 6.7 | 7.6 | 5.9 | 6.3 | 6.1 | 5.2 | 4.9 | 5.0 | 5.8 | 7.1 | 7.5 | 7.2 | 9.1 | 10.5 | 8.7 | 6.7 | 6.9 | 6.9 |
Std Deviation (1e-4) | 1.1 | 1.5 | 1.6 | 1.7 | 2.3 | 1.7 | 1.6 | 1.6 | 1.3 | 1.2 | 1.3 | 1.5 | 2.2 | 2.2 | 2.6 | 2.8 | 3.1 | 2.8 | 1.6 | 1.8 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.14 | -0.23 | -0.13 | -0.18 | -0.41 | -0.49 | -0.46 | -0.26 | 0.04 | 0.13 | 0.07 | 0.4 | 0.6 | 0.34 | -0.04 | 0.0 | 0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 4.4 | 3.4 | 4.3 | 3.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.1 | 4.8 | 5.0 | 2.6 | 3.5 | 3.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.8 | 0.5 | 3.2 | 1.4 | 0.6 | 0 | -2.2 | -0.9 | -0.5 | -1.0 | 2.3 | 1.6 | 1.5 | 2.5 | 1.4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -0.4 | 0.5 | 4.7 | 3.6 | 1.6 | 7.0 | 8.1 | 7.5 | 4.1 | 3.4 | 0.7 | 1.0 | 1.5 | 1.3 | 3.5 |
Nucleus | -3.4 | -3.5 | -3.8 | -4.1 | -4.0 | -4.1 | -5.2 | -5.8 | -2.8 | -2.3 | -3.9 | -4.2 | 0.1 | -2.6 | 0.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.0 | 2.3 |
Nucleolus | 3.4 | 1.0 | 1.4 | 2.9 | 2.9 | 4.1 | 3.1 | 3.6 | 2.5 | 1.8 | 1.7 | 1.7 | -1.1 | 0.2 | -2.7 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1.6 | -1.4 | -2.6 | 0 | -2.7 | -3.6 | -2.4 | -2.5 | -5.2 | -3.2 | -2.0 | -4.2 | -4.2 | -4.2 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.1 | 2.1 | 2.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.3637 | 6.2664 | 6.0713 | 4.9828 | 5.258 | 5.9918 | 9.7903 | 18.6943 | 8.5981 | 7.4412 | 6.8465 | 7.7922 | 8.3702 | 6.9471 | 7.1107 | 6.4064 | 6.4936 | 6.808 |
Actin | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0.003 | 0 | 0.002 | 0 | 0 | 0.0006 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0007 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0003 |
Bud Periphery | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0012 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0008 | 0.0001 | 0.0009 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0.0025 | 0.0145 | 0.0076 | 0 | 0 | 0.0088 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasmic Foci | 0 | 0 | 0 | 0.0001 | 0.0005 | 0.0001 | 0.006 | 0 | 0.007 | 0.0001 | 0.0003 | 0.003 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0.0003 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Lipid Particles | 0 | 0.0001 | 0.0001 | 0 | 0.0018 | 0.0002 | 0.0009 | 0 | 0.0003 | 0.0001 | 0.0043 | 0.0005 | 0.0001 | 0 | 0.0008 | 0.0001 | 0.0002 | 0.0001 |
Mitochondria | 0.0002 | 0.0001 | 0 | 0 | 0.0003 | 0.0001 | 0.0007 | 0.0001 | 0.0021 | 0.0001 | 0.0005 | 0.0006 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 |
None | 0 | 0 | 0 | 0 | 0.0001 | 0.0005 | 0.002 | 0 | 0.0049 | 0 | 0.0001 | 0.0008 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0004 | 0.0023 | 0.0008 | 0.0001 | 0.0041 | 0.0009 | 0.0008 | 0.0041 | 0.0012 | 0.0054 | 0.0076 | 0.0012 | 0.0006 | 0.0008 | 0.0009 | 0.0007 | 0.0024 | 0.0001 |
Nucleolus | 0.5292 | 0.4451 | 0.4982 | 0.504 | 0.6251 | 0.6209 | 0.6996 | 0.5304 | 0.6446 | 0.6042 | 0.7232 | 0.6907 | 0.6135 | 0.4229 | 0.5858 | 0.6517 | 0.673 | 0.6881 |
Nucleus | 0.4642 | 0.543 | 0.494 | 0.4082 | 0.2859 | 0.3631 | 0.2654 | 0.4377 | 0.3089 | 0.3797 | 0.2232 | 0.2739 | 0.3674 | 0.5489 | 0.392 | 0.3229 | 0.2642 | 0.2938 |
Peroxisomes | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0055 | 0.0057 | 0.0065 | 0.0872 | 0.0802 | 0.0135 | 0.0157 | 0.0111 | 0.0188 | 0.0099 | 0.0384 | 0.0181 | 0.0172 | 0.0273 | 0.0196 | 0.0244 | 0.0594 | 0.0175 |
Vacuole | 0.0001 | 0.0021 | 0 | 0 | 0.0002 | 0.0001 | 0.0006 | 0.001 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Vacuole Periphery | 0.0001 | 0.0009 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 36.5413 | 25.6202 | 26.9326 | 42.1844 | 47.3774 | 37.2372 | 21.803 | 21.8548 | 38.7872 | 43.0445 |
Translational Efficiency | 1.4994 | 1.3522 | 1.2528 | 1.4139 | 1.2216 | 1.3759 | 1.1397 | 0.9283 | 0.9447 | 1.1524 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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